Antibacterial activity of liposome encapsulated cyclo(TYR-PRO)
- Authors: Tshanga, Siphokazi Sisanda
- Date: 2011
- Subjects: Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10132 , http://hdl.handle.net/10948/1450 , Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Description: Cyclic dipeptides (CDPs) are amino acid-based compounds, some of which possess antibacterial activity. The encapsulation of certain drugs into liposomes has been found to improve their activity in terms of bioavailability and duration of action. Liposomes are small vesicles that are under investigation as drug carriers for the delivery of therapeutic agents. A number of liposome formulations are currently under clinical trial review, whilst some have already been approved for clinical use. The aim of this study was to optimize a liposomal cyclo(Tyr-Pro) formulation and to assess its antibacterial activity against various Gram-positive and Gram-negative bacteria. Response surface methodology (RSM) using the central composite design (CCD) model was used to optimize liposomal formulations of cyclo(Tyr-Pro) for each of the four bacteria, namely Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Percent drug encapsulated and bacterial inhibition were investigated with respect to two independent variables, i.e. lipid composition and cholesterol content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each bacterium. The model selected by the software failed to adequately correlate the predicted models to the experimental data. The in vitro experiments showed that the antibacterial activity of liposome-encapsulated cyclo(Tyr-Pro) was superior to that of its free counterpart. Binding maximum or Bmax for the encapsulated compound at concentrations as low as 0.412 mg/ml, was significantly higher than that obtained for free cyclo(Tyr-Pro) which was tested at a concentration of 20 mg/ml. Furthermore, encapsulation of cyclo(Tyr-Pro) into a liposome formulation enhanced its potency. This was evident in the lower IC50 values for the liposomal compound when compared to free cyclo(Tyr-Pro).
- Full Text:
- Date Issued: 2011
- Authors: Tshanga, Siphokazi Sisanda
- Date: 2011
- Subjects: Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10132 , http://hdl.handle.net/10948/1450 , Peptide antibiotics , Antibacterial agents -- Therapeutic use -- Testing
- Description: Cyclic dipeptides (CDPs) are amino acid-based compounds, some of which possess antibacterial activity. The encapsulation of certain drugs into liposomes has been found to improve their activity in terms of bioavailability and duration of action. Liposomes are small vesicles that are under investigation as drug carriers for the delivery of therapeutic agents. A number of liposome formulations are currently under clinical trial review, whilst some have already been approved for clinical use. The aim of this study was to optimize a liposomal cyclo(Tyr-Pro) formulation and to assess its antibacterial activity against various Gram-positive and Gram-negative bacteria. Response surface methodology (RSM) using the central composite design (CCD) model was used to optimize liposomal formulations of cyclo(Tyr-Pro) for each of the four bacteria, namely Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Percent drug encapsulated and bacterial inhibition were investigated with respect to two independent variables, i.e. lipid composition and cholesterol content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each bacterium. The model selected by the software failed to adequately correlate the predicted models to the experimental data. The in vitro experiments showed that the antibacterial activity of liposome-encapsulated cyclo(Tyr-Pro) was superior to that of its free counterpart. Binding maximum or Bmax for the encapsulated compound at concentrations as low as 0.412 mg/ml, was significantly higher than that obtained for free cyclo(Tyr-Pro) which was tested at a concentration of 20 mg/ml. Furthermore, encapsulation of cyclo(Tyr-Pro) into a liposome formulation enhanced its potency. This was evident in the lower IC50 values for the liposomal compound when compared to free cyclo(Tyr-Pro).
- Full Text:
- Date Issued: 2011
Antioxidant activity and the quality of meat from goats and broilers supplemented with Moringa (Moringa Oleifera) leaves
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
Applicability of public-private partnership in provision of water infrastructure to central coastal Namibia mines
- Kavela, Lukas Tangeni Taukondjo
- Authors: Kavela, Lukas Tangeni Taukondjo
- Date: 2011
- Subjects: Water-supply -- Namibia , Public-private sector cooperation -- Namibia -- Water-supply
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9701 , http://hdl.handle.net/10948/d1015641
- Description: This study investigated the potential application of Public-Private Partnerships (PPPs) in water supply, taking cognisance of the water demand and supply in the Central Coastal Namibian (CCN) mines. NamWater does not meet demand for the existing and planned mines in the CCN. Primary objective was to investigate and analyse water demand and supply as well as the effectiveness of PPP as a solution by exploring its potential, successes and challenges. In order to obtain relevant information, literature review was used to collect evidence on water demand and supply in the north-central Namibia as well as the application of PPP in provision of water supply. Multi-case studies were carried out on three operational mines, namely; Rio Tinto Rössing Uranium Mine, Langer Heinrich Uranium Mine and Areva Trekkopje Uranium Mine to attain empirical data on water supply requirements. A self-administered questionnaire was used to collect the data supplemented by interviews with NamWater, operational mines within the study area, government institutions, and knowledgeable consultants on water and the mining environment. The key findings included: institutional weaknesses and lack of legislation frameworks to support PPP projects; skills shortage; and financing problems were identified as major challenges. Results further show that these problems could be resolved by effectively implementing a PPP approach, whereby the Special Purpose Vehicle (SPV) enters into a contract with the public authority. The study concludes that there is a need to increase water output to meet current and future supply requirements to these mines. This situation should be addressed by all stakeholders, from government to private sectors. The study recommends that a sea water desalination plant should be developed to increase water capacity supply to the existing operational and prospective mines in the area. The PPP approach is proposed as a possible solution to water supply infrastructure service delivery problem to ensure long-term water security in the CCN mines.
- Full Text:
- Date Issued: 2011
- Authors: Kavela, Lukas Tangeni Taukondjo
- Date: 2011
- Subjects: Water-supply -- Namibia , Public-private sector cooperation -- Namibia -- Water-supply
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9701 , http://hdl.handle.net/10948/d1015641
- Description: This study investigated the potential application of Public-Private Partnerships (PPPs) in water supply, taking cognisance of the water demand and supply in the Central Coastal Namibian (CCN) mines. NamWater does not meet demand for the existing and planned mines in the CCN. Primary objective was to investigate and analyse water demand and supply as well as the effectiveness of PPP as a solution by exploring its potential, successes and challenges. In order to obtain relevant information, literature review was used to collect evidence on water demand and supply in the north-central Namibia as well as the application of PPP in provision of water supply. Multi-case studies were carried out on three operational mines, namely; Rio Tinto Rössing Uranium Mine, Langer Heinrich Uranium Mine and Areva Trekkopje Uranium Mine to attain empirical data on water supply requirements. A self-administered questionnaire was used to collect the data supplemented by interviews with NamWater, operational mines within the study area, government institutions, and knowledgeable consultants on water and the mining environment. The key findings included: institutional weaknesses and lack of legislation frameworks to support PPP projects; skills shortage; and financing problems were identified as major challenges. Results further show that these problems could be resolved by effectively implementing a PPP approach, whereby the Special Purpose Vehicle (SPV) enters into a contract with the public authority. The study concludes that there is a need to increase water output to meet current and future supply requirements to these mines. This situation should be addressed by all stakeholders, from government to private sectors. The study recommends that a sea water desalination plant should be developed to increase water capacity supply to the existing operational and prospective mines in the area. The PPP approach is proposed as a possible solution to water supply infrastructure service delivery problem to ensure long-term water security in the CCN mines.
- Full Text:
- Date Issued: 2011
Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A
- Authors: Flanagan, Shane Patrick
- Date: 2011 , 2010-12-06
- Subjects: Ochratoxins , Filamentous fungi , Electrochemical sensors , Nanostructured materials , Catalysts , Food contamination
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4121 , http://hdl.handle.net/10962/d1013328
- Description: Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine. Produced as a secondary fungal metabolite by several species of Aspergillus and Penicillium, ochratoxin A has been shown to readily contaminate a large variety of commodities including cereals, groundnuts, dried fruit, spices and coffee. This has led to widespread contamination of ochratoxin in wine, beer, milk and meat products. As ochratoxin A is a potent nephrotoxin exhibiting teratogenic and carcinogenic properties, the development of a rapid screening platform for the cost effective control of ochratoxin A content in foodstuffs is therefore required. The evaluation of metallophthalocyanine and carbon nanotube electrode modification toward the development of a nanostructured biosensor capable of enhancing the electrochemical detection of ochratoxin A in complex media is presented. Cyclic voltammetry at a glassy carbon electrode allowed for the optimization of detection parameters including pH and type of supporting electrolyte. Britton-Robinson buffer was found to be the most suitable supporting electrolyte in terms of sensitivity and reproducibility obtaining a LOD of 0.28 μM as determined by differential pulse voltammetry. Subsequent analysis determined the dependence of OTA oxidation on pH in acidic media which proceeds with the transfer of two electrons to form a quinone/hydroquinone couple shown to adsorb to the electrode surface. Passivation of the electrode through adsorption of oxidation products was shown to severely limit the detection of OTA upon successive detection cycles. Comparison of various metallophthalocyanine modifiers showed an increase in sensitivity toward the detection of OTA at phthalocyanine complexes with metal based redox processes. However with the exception of NiPc and CoTCPc complexes, phthalocyanine modification was limited by the increase in deviation of current response and extent of fouling. NiPc modification showed an increase in sensitivity by two fold with fouling characteristics comparable to an unmodified electrode while low improvements in fouling was observed at CoTCPc modified electrodes with sensitivity in detection comparable to an unmodified electrode.Modification of the electrode with multi- and single walled carbon nanotubes produced a significant increase in sensitivity toward the detection of ochratoxin A. The electrocatalytic activity of nanotube modifiers was attributed to the increase in surface area and to the addition of oxygenated functional groups upon acid treatment as confirmed by Raman spectroscopy. Acid functionalization of the carbon nanotubes for a period of two hours produced the greatest increase in sensitivity obtaining a respective LOD of 0.09 μM and 0.03 μM for analysis of ochratoxin A at multi- and single walled carbon nanotube modified electrodes. Centrifugal purification of carbon nanotubes was deemed necessary to improve the electrocatalytic activity of the nanotube modifiers through the removal of carbonaceous impurities as visualized by atomic force microscopy. Furthermore, a crude lipase preparation, lipase A, was investigated as a potential biological recognition element for selective detection of ochratoxin A in complex media. Lipase A enabled the hydrolysis of ochratoxin A to the electroactive species ochratoxin α as confirmed by thin layer chromatography and voltammetric analysis. Additional isolation of a pure hydrolase from the lipase A preparation is required prior to utilization within a nanostructured biosensor platform capable of detecting ochratoxin A in complex media.
- Full Text:
- Date Issued: 2011
- Authors: Flanagan, Shane Patrick
- Date: 2011 , 2010-12-06
- Subjects: Ochratoxins , Filamentous fungi , Electrochemical sensors , Nanostructured materials , Catalysts , Food contamination
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4121 , http://hdl.handle.net/10962/d1013328
- Description: Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine. Produced as a secondary fungal metabolite by several species of Aspergillus and Penicillium, ochratoxin A has been shown to readily contaminate a large variety of commodities including cereals, groundnuts, dried fruit, spices and coffee. This has led to widespread contamination of ochratoxin in wine, beer, milk and meat products. As ochratoxin A is a potent nephrotoxin exhibiting teratogenic and carcinogenic properties, the development of a rapid screening platform for the cost effective control of ochratoxin A content in foodstuffs is therefore required. The evaluation of metallophthalocyanine and carbon nanotube electrode modification toward the development of a nanostructured biosensor capable of enhancing the electrochemical detection of ochratoxin A in complex media is presented. Cyclic voltammetry at a glassy carbon electrode allowed for the optimization of detection parameters including pH and type of supporting electrolyte. Britton-Robinson buffer was found to be the most suitable supporting electrolyte in terms of sensitivity and reproducibility obtaining a LOD of 0.28 μM as determined by differential pulse voltammetry. Subsequent analysis determined the dependence of OTA oxidation on pH in acidic media which proceeds with the transfer of two electrons to form a quinone/hydroquinone couple shown to adsorb to the electrode surface. Passivation of the electrode through adsorption of oxidation products was shown to severely limit the detection of OTA upon successive detection cycles. Comparison of various metallophthalocyanine modifiers showed an increase in sensitivity toward the detection of OTA at phthalocyanine complexes with metal based redox processes. However with the exception of NiPc and CoTCPc complexes, phthalocyanine modification was limited by the increase in deviation of current response and extent of fouling. NiPc modification showed an increase in sensitivity by two fold with fouling characteristics comparable to an unmodified electrode while low improvements in fouling was observed at CoTCPc modified electrodes with sensitivity in detection comparable to an unmodified electrode.Modification of the electrode with multi- and single walled carbon nanotubes produced a significant increase in sensitivity toward the detection of ochratoxin A. The electrocatalytic activity of nanotube modifiers was attributed to the increase in surface area and to the addition of oxygenated functional groups upon acid treatment as confirmed by Raman spectroscopy. Acid functionalization of the carbon nanotubes for a period of two hours produced the greatest increase in sensitivity obtaining a respective LOD of 0.09 μM and 0.03 μM for analysis of ochratoxin A at multi- and single walled carbon nanotube modified electrodes. Centrifugal purification of carbon nanotubes was deemed necessary to improve the electrocatalytic activity of the nanotube modifiers through the removal of carbonaceous impurities as visualized by atomic force microscopy. Furthermore, a crude lipase preparation, lipase A, was investigated as a potential biological recognition element for selective detection of ochratoxin A in complex media. Lipase A enabled the hydrolysis of ochratoxin A to the electroactive species ochratoxin α as confirmed by thin layer chromatography and voltammetric analysis. Additional isolation of a pure hydrolase from the lipase A preparation is required prior to utilization within a nanostructured biosensor platform capable of detecting ochratoxin A in complex media.
- Full Text:
- Date Issued: 2011
Application of lean manufacturing tools in cash centres to improve operational efficiency
- Authors: Smith, Ryan Erich
- Date: 2011
- Subjects: Lean manufacturing
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8647 , http://hdl.handle.net/10948/1391 , Lean manufacturing
- Description: Financial institutes, typically banks, that derive funding from the collection of cash deposits, could derive benefit from the research. In addition, companies who act as outsourced suppliers to such institutes, and therefore process cash deposits on behalf of the banks should derive a similar benefit. Notably, some financial institutes outsource their cash management services. The advantages of outsourcing, such as freeing up staff, and getting the organisation to focus on its core activities, has to be weighed against the challenges that it provides. Hines et al. (2000: 17) note that outsourcing often means a heavy reliance on third party for service, the reliance on third parties 7 for the managing of funds, as well as the risk of securing customer confidentiality. Referring to a survey done by Ernst and Young in 1999, Hines et al. (2000: 15) revealed that revenue from cash management had grown from $7 billion in 1993, to $10 billion in 1999. It would thus be hard to overstate the significance of this industry, or the importance of gaining a competitive advantage. This research aims to provide some guideline on how the banks could become more competitive through pricing and service without cross-subsidising their bulk cash processing divisions. Competitiveness, in turn, would be derived by improving productivity through the application and transfer of lean tools from manufacturing and the service industry. Although the lean approach is well established in the manufacturing sector and certain product-service sectors, evidence of lean in pure service environments is very limited (Piercy & Rich, 2009: 59). Cash centres have a unique combination of service and product environments in the sense that: (1) cash is the commodity and (2) the commodity received from customers is in a sense used as raw material to create products like floats, payrolls, and cash for ATMs. This research could therefore contribute to the way management views the suitability of lean production methodologies in the context of a part product and part service environment such as the cash centre.
- Full Text:
- Date Issued: 2011
- Authors: Smith, Ryan Erich
- Date: 2011
- Subjects: Lean manufacturing
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8647 , http://hdl.handle.net/10948/1391 , Lean manufacturing
- Description: Financial institutes, typically banks, that derive funding from the collection of cash deposits, could derive benefit from the research. In addition, companies who act as outsourced suppliers to such institutes, and therefore process cash deposits on behalf of the banks should derive a similar benefit. Notably, some financial institutes outsource their cash management services. The advantages of outsourcing, such as freeing up staff, and getting the organisation to focus on its core activities, has to be weighed against the challenges that it provides. Hines et al. (2000: 17) note that outsourcing often means a heavy reliance on third party for service, the reliance on third parties 7 for the managing of funds, as well as the risk of securing customer confidentiality. Referring to a survey done by Ernst and Young in 1999, Hines et al. (2000: 15) revealed that revenue from cash management had grown from $7 billion in 1993, to $10 billion in 1999. It would thus be hard to overstate the significance of this industry, or the importance of gaining a competitive advantage. This research aims to provide some guideline on how the banks could become more competitive through pricing and service without cross-subsidising their bulk cash processing divisions. Competitiveness, in turn, would be derived by improving productivity through the application and transfer of lean tools from manufacturing and the service industry. Although the lean approach is well established in the manufacturing sector and certain product-service sectors, evidence of lean in pure service environments is very limited (Piercy & Rich, 2009: 59). Cash centres have a unique combination of service and product environments in the sense that: (1) cash is the commodity and (2) the commodity received from customers is in a sense used as raw material to create products like floats, payrolls, and cash for ATMs. This research could therefore contribute to the way management views the suitability of lean production methodologies in the context of a part product and part service environment such as the cash centre.
- Full Text:
- Date Issued: 2011
Application of macroinvertebrate based biomonitoring approaches to assess anthropogenic impacts in the Swartkops River, South Africa
- Authors: Odume, Oghenekaro Nelson
- Date: 2011
- Subjects: Water -- Pollution -- South Africa -- Swartkops River Water quality biological assessment -- South Africa -- Swartkops River Environmental monitoring -- South Africa -- Swartkops River Aquatic invertebrates -- Effect of water pollution on -- South Africa -- Swartkops River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6040 , http://hdl.handle.net/10962/d1006199
- Description: A growing human population accompanied by urbanisation and industrialisation have led to over exploitation and pollution of freshwater resources and have consequently impacted on aquatic ecosystem health. The Swartkops River in the Eastern Cape of South Africa is no exception. It drains a heavily industrialised catchment which has led to deterioration of its water quality due to pollution. Integrated water resources management (IWRM) requires the concurrent sustainable use of water resources and the protection of aquatic ecosystem health. Macroinvertebrates are well known for their ability to reflect the health of the environment in which they live, thus they were used to assess anthropogenic impacts in the Swartkops River for this study. Macroinvertebrate based biomonitoring approaches, including the South African Scoring System version 5 (SASS5); a multimetric approach involving 19 metrics; Chironomidae community assessments and screening of morphological deformities in Chironomidae larvae, were applied at four selected sampling sites to assess environmental water quality in the Swartkops River. Macroinvertebrates were sampled us ing the SASS5 protocols. Chironomidae were mounted and identified as far as practically possible using available keys. Mentum, ligula, mandible, paraligula and antenna in Chironomidae larvae were screened for deformities. Physical and chemical water quality variables were measured at each of the selected sampling sites. All data were subjected to relevant statistical analyses. Of the four sites sampled during the study period, results revealed that water quality at site 1 was the least impacted with highest SASS5 scores, average score per taxa (ASPT) values, richness, diversity, equitability and Ephemeroptera –Plecoptera-Trichoptera (EPT) richness, as well as least incidences of chironomid deformities. Water quality at site 2 was considered the next least impacted with higher SASS5 scores, A SPT values, richness, diversity and equitability, and lower incidences of deformities compared to sites 3 and 4. SASS5 scores and ASPT values revealed that both sites 3 and 4 were critically modified but the multimetric analysis, Chironomidae community assessment and incidences of deformities in Chironomidae larvae indicated that site 3 is the most impacted of the four sampling sites, with least species diversity, richness, equitability and highest incidences of deformities. The study revealed the importance of multicriteria approach to environmental biomonitoring as an integrated water resources management tool, and based on the results, site 3, as the most impacted, could be prioritised for restoration intervention.
- Full Text:
- Date Issued: 2011
- Authors: Odume, Oghenekaro Nelson
- Date: 2011
- Subjects: Water -- Pollution -- South Africa -- Swartkops River Water quality biological assessment -- South Africa -- Swartkops River Environmental monitoring -- South Africa -- Swartkops River Aquatic invertebrates -- Effect of water pollution on -- South Africa -- Swartkops River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:6040 , http://hdl.handle.net/10962/d1006199
- Description: A growing human population accompanied by urbanisation and industrialisation have led to over exploitation and pollution of freshwater resources and have consequently impacted on aquatic ecosystem health. The Swartkops River in the Eastern Cape of South Africa is no exception. It drains a heavily industrialised catchment which has led to deterioration of its water quality due to pollution. Integrated water resources management (IWRM) requires the concurrent sustainable use of water resources and the protection of aquatic ecosystem health. Macroinvertebrates are well known for their ability to reflect the health of the environment in which they live, thus they were used to assess anthropogenic impacts in the Swartkops River for this study. Macroinvertebrate based biomonitoring approaches, including the South African Scoring System version 5 (SASS5); a multimetric approach involving 19 metrics; Chironomidae community assessments and screening of morphological deformities in Chironomidae larvae, were applied at four selected sampling sites to assess environmental water quality in the Swartkops River. Macroinvertebrates were sampled us ing the SASS5 protocols. Chironomidae were mounted and identified as far as practically possible using available keys. Mentum, ligula, mandible, paraligula and antenna in Chironomidae larvae were screened for deformities. Physical and chemical water quality variables were measured at each of the selected sampling sites. All data were subjected to relevant statistical analyses. Of the four sites sampled during the study period, results revealed that water quality at site 1 was the least impacted with highest SASS5 scores, average score per taxa (ASPT) values, richness, diversity, equitability and Ephemeroptera –Plecoptera-Trichoptera (EPT) richness, as well as least incidences of chironomid deformities. Water quality at site 2 was considered the next least impacted with higher SASS5 scores, A SPT values, richness, diversity and equitability, and lower incidences of deformities compared to sites 3 and 4. SASS5 scores and ASPT values revealed that both sites 3 and 4 were critically modified but the multimetric analysis, Chironomidae community assessment and incidences of deformities in Chironomidae larvae indicated that site 3 is the most impacted of the four sampling sites, with least species diversity, richness, equitability and highest incidences of deformities. The study revealed the importance of multicriteria approach to environmental biomonitoring as an integrated water resources management tool, and based on the results, site 3, as the most impacted, could be prioritised for restoration intervention.
- Full Text:
- Date Issued: 2011
Assessing BEE policy's viability in advancing establishment and survival of SMMes in South Africa
- Authors: Waithaka, Stephen Kiumi
- Date: 2011
- Subjects: Small business -- South Africa , Economic development -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/4376 , vital:20591
- Description: Black Economic Empowerment is an ideology and policy that was effected so as to streamline the inequalities that were and have been left to stand after the apartheid era in South Africa. Despite the mixed reactions that would surface as a result of the underpinnings of the policy, the main focus of the policy was and still is to provide for equality and build better business among the economic minority who in this case are the “black” people of South Africa. This report focuses on the policy and the different guidelines that have been set in place to counter the inequalities in businesses and economy with focus being on SMMEs and looking at ways in which various sections, especially the score card, with regard to exemptions to small businesses, how this aids them in achieving BEE compliance. The research accomplished this by looking at the requirements of the score card, focusing mainly on the stipulations placed on SMMEs and by the use of literary reports gathered information that would show whether the exemptions give the said enterprises added competitive advantage. The research produced a couple of findings; that although theoretically the necessity of boosting economic growth and business of one of the most productive enterprise sectors in an economy and the fact that the score card has focused infinitely on the need for SMMEs not to be fully focused on a specific cluster of criterion in the score card but more on which criterion would work best for them and which ones are easy to maneuver around to ensure maximum growth and contribution, there is the loop hole that is experienced of how this criterion is met and passed with disregard to actualization of the need to adhere to the strictness of the rules of how compliance is attained. There was also the main issue of fronting which is the falsification of ownership of a company by providing misleading information about the directors and owners of the business. The main conclusions drawn from this research are that it is important not to lose the foresight that the policy is meant to enhance and grow the economic ability of a sector and build business through which the policy makes very heavy recommendations but there are shortfalls experienced with regard to how compliance is approached putting into consideration that SMMEs have been given exemptions in the number of pillars of the score card they can apply to their business. It is also important to note that the influence and bulldozing that is expected when it comes to dealing with policies of this nature is not to be sidelined. This research aims to look at the discrepancies that are set out within the structures that hold the policy together so as to attain maximum results.
- Full Text:
- Date Issued: 2011
- Authors: Waithaka, Stephen Kiumi
- Date: 2011
- Subjects: Small business -- South Africa , Economic development -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/4376 , vital:20591
- Description: Black Economic Empowerment is an ideology and policy that was effected so as to streamline the inequalities that were and have been left to stand after the apartheid era in South Africa. Despite the mixed reactions that would surface as a result of the underpinnings of the policy, the main focus of the policy was and still is to provide for equality and build better business among the economic minority who in this case are the “black” people of South Africa. This report focuses on the policy and the different guidelines that have been set in place to counter the inequalities in businesses and economy with focus being on SMMEs and looking at ways in which various sections, especially the score card, with regard to exemptions to small businesses, how this aids them in achieving BEE compliance. The research accomplished this by looking at the requirements of the score card, focusing mainly on the stipulations placed on SMMEs and by the use of literary reports gathered information that would show whether the exemptions give the said enterprises added competitive advantage. The research produced a couple of findings; that although theoretically the necessity of boosting economic growth and business of one of the most productive enterprise sectors in an economy and the fact that the score card has focused infinitely on the need for SMMEs not to be fully focused on a specific cluster of criterion in the score card but more on which criterion would work best for them and which ones are easy to maneuver around to ensure maximum growth and contribution, there is the loop hole that is experienced of how this criterion is met and passed with disregard to actualization of the need to adhere to the strictness of the rules of how compliance is attained. There was also the main issue of fronting which is the falsification of ownership of a company by providing misleading information about the directors and owners of the business. The main conclusions drawn from this research are that it is important not to lose the foresight that the policy is meant to enhance and grow the economic ability of a sector and build business through which the policy makes very heavy recommendations but there are shortfalls experienced with regard to how compliance is approached putting into consideration that SMMEs have been given exemptions in the number of pillars of the score card they can apply to their business. It is also important to note that the influence and bulldozing that is expected when it comes to dealing with policies of this nature is not to be sidelined. This research aims to look at the discrepancies that are set out within the structures that hold the policy together so as to attain maximum results.
- Full Text:
- Date Issued: 2011
Assessing challenges in public appointments and recruitment processes in Chris Hani District Municipality: a case study of human resource department in Lukhanji Local Municipality
- Authors: Gijana, Andile Patrick
- Date: 2011
- Subjects: Civil service -- South Africa -- Eastern Cape -- Case studies , Human capital -- South Africa -- Eastern Cape -- Case studies
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11617 , http://hdl.handle.net/10353/325 , Civil service -- South Africa -- Eastern Cape -- Case studies , Human capital -- South Africa -- Eastern Cape -- Case studies
- Description: The aim of this study was to assess challenges in Public Appointments and Recruitment Processes in Chris Hani District Municipality: A case study of Human Resource Department in Lukhanji Local Municipality (2008-2010) of the Eastern Cape Province. There has never been a detailed research study conducted in the Chris Hani Human Resource Department, regarding the subject in question. The Constitution of the Republic of South Africa in Section 195(i) directs that Public Administration must be broadly representative of the South African people, with employment and personnel management practices based on ability, objectivity, fairness and the need to redress the imbalances of the past to achieve broad representation. The literature review explored extensively the models used in implementing recruitment and appointment processes globally. From those tested models elsewhere, it was evident that South Africa has a great Constitution and good policies regarding Local Government Human Resource Department recruitment and appointment processes not withstanding some challenges cited in the study. This assessment of challenges in the public appointments and recruitment processes in Chris Hani District Municipality assisted in the establishment of the real facts about effective and fair recruitment and appointment processes in the Local Government Human Resource Management Department to deserving communities. It became clear from the study that providing efficient and fair recruitment and appointments processes to the Local Government Human Resource Department in South Africa requires a broader advocacy agenda encompassing the development of Human Resource systems and the generation of numerous skills and expertise to deliver services to the poor people of our country.
- Full Text:
- Date Issued: 2011
- Authors: Gijana, Andile Patrick
- Date: 2011
- Subjects: Civil service -- South Africa -- Eastern Cape -- Case studies , Human capital -- South Africa -- Eastern Cape -- Case studies
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11617 , http://hdl.handle.net/10353/325 , Civil service -- South Africa -- Eastern Cape -- Case studies , Human capital -- South Africa -- Eastern Cape -- Case studies
- Description: The aim of this study was to assess challenges in Public Appointments and Recruitment Processes in Chris Hani District Municipality: A case study of Human Resource Department in Lukhanji Local Municipality (2008-2010) of the Eastern Cape Province. There has never been a detailed research study conducted in the Chris Hani Human Resource Department, regarding the subject in question. The Constitution of the Republic of South Africa in Section 195(i) directs that Public Administration must be broadly representative of the South African people, with employment and personnel management practices based on ability, objectivity, fairness and the need to redress the imbalances of the past to achieve broad representation. The literature review explored extensively the models used in implementing recruitment and appointment processes globally. From those tested models elsewhere, it was evident that South Africa has a great Constitution and good policies regarding Local Government Human Resource Department recruitment and appointment processes not withstanding some challenges cited in the study. This assessment of challenges in the public appointments and recruitment processes in Chris Hani District Municipality assisted in the establishment of the real facts about effective and fair recruitment and appointment processes in the Local Government Human Resource Management Department to deserving communities. It became clear from the study that providing efficient and fair recruitment and appointments processes to the Local Government Human Resource Department in South Africa requires a broader advocacy agenda encompassing the development of Human Resource systems and the generation of numerous skills and expertise to deliver services to the poor people of our country.
- Full Text:
- Date Issued: 2011
Assessing challenges in public appointments and recruitment processes in Chris Hani District Municipality: a case study of human resource department in Lukhanji Local Municipality
- Authors: Gijana, Andile Patrick
- Date: 2011
- Subjects: Civil service Human capital -- South Africa -- Eastern Cape South Africa -- Officials and employees -- Selection and appointment
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/5752 , vital:29379
- Description: The aim of this study was to assess challenges in Public Appointments and Recruitment Processes in Chris Hani District Municipality: A case study of Human Resource Department in Lukhanji Local Municipality (2008-2010) of the Eastern Cape Province. There has never been a detailed research study conducted in the Chris Hani Human Resource Department, regarding the subject in question. The Constitution of the Republic of South Africa in Section 195(i) directs that Public Administration must be broadly representative of the South African people, with employment and personnel management practices based on ability, objectivity, fairness and the need to redress the imbalances of the past to achieve broad representation. The literature review explored extensively the models used in implementing recruitment and appointment processes globally. From those tested models elsewhere, it was evident that South Africa has a great Constitution and good policies regarding Local Government Human Resource Department recruitment and appointment processes not withstanding some challenges cited in the study. This assessment of challenges in the public appointments and recruitment processes in Chris Hani District Municipality assisted in the establishment of the real facts about effective and fair recruitment and appointment processes in the Local Government Human Resource Management Department to deserving communities. It became clear from the study that providing efficient and fair recruitment and appointments processes to the Local Government Human Resource Department in South Africa requires a broader advocacy agenda encompassing the development of Human Resource systems and the generation of numerous skills and expertise to deliver services to the poor people of our country.
- Full Text:
- Date Issued: 2011
- Authors: Gijana, Andile Patrick
- Date: 2011
- Subjects: Civil service Human capital -- South Africa -- Eastern Cape South Africa -- Officials and employees -- Selection and appointment
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/5752 , vital:29379
- Description: The aim of this study was to assess challenges in Public Appointments and Recruitment Processes in Chris Hani District Municipality: A case study of Human Resource Department in Lukhanji Local Municipality (2008-2010) of the Eastern Cape Province. There has never been a detailed research study conducted in the Chris Hani Human Resource Department, regarding the subject in question. The Constitution of the Republic of South Africa in Section 195(i) directs that Public Administration must be broadly representative of the South African people, with employment and personnel management practices based on ability, objectivity, fairness and the need to redress the imbalances of the past to achieve broad representation. The literature review explored extensively the models used in implementing recruitment and appointment processes globally. From those tested models elsewhere, it was evident that South Africa has a great Constitution and good policies regarding Local Government Human Resource Department recruitment and appointment processes not withstanding some challenges cited in the study. This assessment of challenges in the public appointments and recruitment processes in Chris Hani District Municipality assisted in the establishment of the real facts about effective and fair recruitment and appointment processes in the Local Government Human Resource Management Department to deserving communities. It became clear from the study that providing efficient and fair recruitment and appointments processes to the Local Government Human Resource Department in South Africa requires a broader advocacy agenda encompassing the development of Human Resource systems and the generation of numerous skills and expertise to deliver services to the poor people of our country.
- Full Text:
- Date Issued: 2011
Assessing communication challenges between clerks and deaf patients at Nelson Mandela Academic Hospital in Mthatha
- Authors: Stemela, Unati
- Date: 2011
- Subjects: Clerks and deaf patients -- Communication -- Hospital
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: vital:18417 , http://hdl.handle.net/11260/d1006597
- Description: A study on assessing communication challenges between clerks and deaf patients at Nelson Mandela Academic Hospital was conducted in 2010. The aim was to assess the existence of communication challenges, possible reasons and solutions to these. The literature reviewed identified a gap in the knowledge of communication between staff and deaf patients. A health systems’ study was conducted using a descriptive, cross sectional survey. The study population was made up of 33 clerks who worked at the registration and records area and deaf patients who stayed at Efata and received health services from the hospital. All clerks were interviewed and a random sample of 106 deaf patients was estimated using Epidat statistical software. Self administered questionnaires were used for data collection. Results confirmed the existence of communication challenges between the two groups. A majority of clerks were not trained in Sign Language. They used a combination of methods to communicate with deaf patients, and few clerks could use Sign Language. The patients also used a combination of methods due to the frustration of not having a common method of communication with clerks. The findings clearly showed that there are communication challenges between the two groups and Sign Language needed to be introduced to clerks in order to accommodate deaf patients. Further research may be done on all healthcare providers and to all deaf patients in the region served by Nelson Mandela Academic Hospital, and this could have a positive impact on the quality of service offered by the hospital to the deaf community.
- Full Text:
- Date Issued: 2011
- Authors: Stemela, Unati
- Date: 2011
- Subjects: Clerks and deaf patients -- Communication -- Hospital
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: vital:18417 , http://hdl.handle.net/11260/d1006597
- Description: A study on assessing communication challenges between clerks and deaf patients at Nelson Mandela Academic Hospital was conducted in 2010. The aim was to assess the existence of communication challenges, possible reasons and solutions to these. The literature reviewed identified a gap in the knowledge of communication between staff and deaf patients. A health systems’ study was conducted using a descriptive, cross sectional survey. The study population was made up of 33 clerks who worked at the registration and records area and deaf patients who stayed at Efata and received health services from the hospital. All clerks were interviewed and a random sample of 106 deaf patients was estimated using Epidat statistical software. Self administered questionnaires were used for data collection. Results confirmed the existence of communication challenges between the two groups. A majority of clerks were not trained in Sign Language. They used a combination of methods to communicate with deaf patients, and few clerks could use Sign Language. The patients also used a combination of methods due to the frustration of not having a common method of communication with clerks. The findings clearly showed that there are communication challenges between the two groups and Sign Language needed to be introduced to clerks in order to accommodate deaf patients. Further research may be done on all healthcare providers and to all deaf patients in the region served by Nelson Mandela Academic Hospital, and this could have a positive impact on the quality of service offered by the hospital to the deaf community.
- Full Text:
- Date Issued: 2011
Assessing conflict and management interventions
- Authors: Plaatjes, Carlton Henry
- Date: 2011
- Subjects: Conflict management -- South Africa , Cognitive styles
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8818 , http://hdl.handle.net/10948/d1018930
- Description: Human resource management, or people management, is concerned with the philosophies, policies, programmes, practices and decisions that affect the people who work for an organisation. The various people management functions are aimed at helping the organisation achieve its strategic goals and as such are an integral part of the management process. People management consists of several aspects and sub-divisions of which pro-active conflict handling and management is one and which is also the subject of this study. The objective of this study was to assess causes of conflict and interventions and styles of conflict management in the workplace. Workplace politics, change management, diversity, cultures and religious views are but a few major sources for the emergence of conflict. We are currently in the era of fast change or more aptly put “hyperchange” and conflict is inevitable and management styles can also create and/or escalate conflict situations including, the composition of diversity in the workplace. This adds to the new challenges of management. Organisations in this decade need to acknowledge that their management styles of days gone by are not relevant anymore and one must understand to recognise conflict and resolve it in an appropriate manner. This study assessed the major causes of conflict in the workplace and whether the managerial style of managers and management interventions impacts on the overall conflict situations experienced by staff members in organisations in Cape Town and Windhoek. It also gave an indication if interventions and conflict management training/programmes are in place or used, if at all. Sometimes conflict is resolved successfully or unsuccessfully and what impact it has on the managers, his/her staff and colleagues and the organisation as a whole. This can have a detriment impact on the business and the culture within the organisation and which could result in or give rise to high labour turnover, underlying unhappy staff and disempowerment of managers and staff and poor production and service levels.
- Full Text:
- Date Issued: 2011
- Authors: Plaatjes, Carlton Henry
- Date: 2011
- Subjects: Conflict management -- South Africa , Cognitive styles
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8818 , http://hdl.handle.net/10948/d1018930
- Description: Human resource management, or people management, is concerned with the philosophies, policies, programmes, practices and decisions that affect the people who work for an organisation. The various people management functions are aimed at helping the organisation achieve its strategic goals and as such are an integral part of the management process. People management consists of several aspects and sub-divisions of which pro-active conflict handling and management is one and which is also the subject of this study. The objective of this study was to assess causes of conflict and interventions and styles of conflict management in the workplace. Workplace politics, change management, diversity, cultures and religious views are but a few major sources for the emergence of conflict. We are currently in the era of fast change or more aptly put “hyperchange” and conflict is inevitable and management styles can also create and/or escalate conflict situations including, the composition of diversity in the workplace. This adds to the new challenges of management. Organisations in this decade need to acknowledge that their management styles of days gone by are not relevant anymore and one must understand to recognise conflict and resolve it in an appropriate manner. This study assessed the major causes of conflict in the workplace and whether the managerial style of managers and management interventions impacts on the overall conflict situations experienced by staff members in organisations in Cape Town and Windhoek. It also gave an indication if interventions and conflict management training/programmes are in place or used, if at all. Sometimes conflict is resolved successfully or unsuccessfully and what impact it has on the managers, his/her staff and colleagues and the organisation as a whole. This can have a detriment impact on the business and the culture within the organisation and which could result in or give rise to high labour turnover, underlying unhappy staff and disempowerment of managers and staff and poor production and service levels.
- Full Text:
- Date Issued: 2011
Assessing education's relevance to industry needs and the impact on graduate employability: a case study of UFH communication graduates
- Authors: Siwela, Ntandokazulu
- Date: 2011
- Subjects: Graduate students -- South Africa , Curriculum-based assessment -- South Africa , Educational evaluation , Employability -- South Africa
- Language: English
- Type: Thesis , Masters , M Soc Sc (Dev)
- Identifier: vital:11425 , http://hdl.handle.net/10353/571 , Graduate students -- South Africa , Curriculum-based assessment -- South Africa , Educational evaluation , Employability -- South Africa
- Description: The 21st century has seen nations becoming more competitive yet ironically more interdependent as their future becomes even more dependent on the knowledge, skills, and resourcefulness of its people, creating new opportunities and difficulties for education, (Power, 2000). The education-work-society interdependency has therefore become more important now than ever. It is also because of this triad-interdependency that tertiary education‟s relevance to industry needs has become a crucial issue in societal development. The ever changing needs of South Africa, the unemployment rate, introduction of new technologies and many other ripple effects of globalization have seen more and more pressure mounting on the South African education system as more and more people try to ensure that the education system answers the crucial challenges of the country today and tomorrow.The discussion below provides a detailed account of the context, in which this discourse on curriculum relevance takes place, highlighting some relevant cornerstones of the Reconstruction and Development Programme, (RDP, 1994). Furthermore, this chapter exposes the key research questions and significance of the study. The subsequent chapter 2 gives an account of what other scholars have said on issues of curriculum relevance, development and the South African Higher Education system. Chapter 3 explains the research methodology as a research process used to investigate the problem in question. In this research study the researcher uses both quantitative and qualitative design (online surveys of three groups, these are the graduates, prospective employers and the lecturers). The analysis is based on quantitative data and further substantiated through qualitative data analysis. The subsequent chapter tabulates and lays out the results according to the surveys. The final chapter discusses the findings and makes future recommendations.
- Full Text:
- Date Issued: 2011
- Authors: Siwela, Ntandokazulu
- Date: 2011
- Subjects: Graduate students -- South Africa , Curriculum-based assessment -- South Africa , Educational evaluation , Employability -- South Africa
- Language: English
- Type: Thesis , Masters , M Soc Sc (Dev)
- Identifier: vital:11425 , http://hdl.handle.net/10353/571 , Graduate students -- South Africa , Curriculum-based assessment -- South Africa , Educational evaluation , Employability -- South Africa
- Description: The 21st century has seen nations becoming more competitive yet ironically more interdependent as their future becomes even more dependent on the knowledge, skills, and resourcefulness of its people, creating new opportunities and difficulties for education, (Power, 2000). The education-work-society interdependency has therefore become more important now than ever. It is also because of this triad-interdependency that tertiary education‟s relevance to industry needs has become a crucial issue in societal development. The ever changing needs of South Africa, the unemployment rate, introduction of new technologies and many other ripple effects of globalization have seen more and more pressure mounting on the South African education system as more and more people try to ensure that the education system answers the crucial challenges of the country today and tomorrow.The discussion below provides a detailed account of the context, in which this discourse on curriculum relevance takes place, highlighting some relevant cornerstones of the Reconstruction and Development Programme, (RDP, 1994). Furthermore, this chapter exposes the key research questions and significance of the study. The subsequent chapter 2 gives an account of what other scholars have said on issues of curriculum relevance, development and the South African Higher Education system. Chapter 3 explains the research methodology as a research process used to investigate the problem in question. In this research study the researcher uses both quantitative and qualitative design (online surveys of three groups, these are the graduates, prospective employers and the lecturers). The analysis is based on quantitative data and further substantiated through qualitative data analysis. The subsequent chapter tabulates and lays out the results according to the surveys. The final chapter discusses the findings and makes future recommendations.
- Full Text:
- Date Issued: 2011
Assessing the 'breaking new ground in housing' policy of South Africa in meeting the objectives of community participation
- Authors: April, Tandeka
- Date: 2011
- Subjects: Housing policy -- Citizen participation , Political participation -- South Africa -- Buffalo City Metropolitan Municipality -- Evaluation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9070 , http://hdl.handle.net/10948/d1008400 , Housing policy -- Citizen participation , Political participation -- South Africa -- Buffalo City Metropolitan Municipality -- Evaluation
- Description: Community participation is an important aspect of South African government policies that deal with integrated development planning and sustainable development. This report presents aspects of community participation in infrastructure delivery and in particular housing in Reeston which is part of the Duncan Village Redevelopment Initiative in East London area of the Buffalo City Metropolitan Municipality. The importance of community participation is acknowledged as it enhances the ability of the community members to demonstrate and use their own views to deal with specific issues and to address the needs and problems that emerge and prevail in their societies. The two emerging paradigms of community participation suggest that the participating communities should be involved in the ‘selection, design, planning and implementation’ of projects that will have an effect on them and from which they are going to benefit ; and that continuous feedback to communities forms a fundamental part of any development activity. This report explores how the “Breaking New Ground in Housing” (BNG) policy of the South African Government meets its objectives in the context of community participation.
- Full Text:
- Date Issued: 2011
- Authors: April, Tandeka
- Date: 2011
- Subjects: Housing policy -- Citizen participation , Political participation -- South Africa -- Buffalo City Metropolitan Municipality -- Evaluation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9070 , http://hdl.handle.net/10948/d1008400 , Housing policy -- Citizen participation , Political participation -- South Africa -- Buffalo City Metropolitan Municipality -- Evaluation
- Description: Community participation is an important aspect of South African government policies that deal with integrated development planning and sustainable development. This report presents aspects of community participation in infrastructure delivery and in particular housing in Reeston which is part of the Duncan Village Redevelopment Initiative in East London area of the Buffalo City Metropolitan Municipality. The importance of community participation is acknowledged as it enhances the ability of the community members to demonstrate and use their own views to deal with specific issues and to address the needs and problems that emerge and prevail in their societies. The two emerging paradigms of community participation suggest that the participating communities should be involved in the ‘selection, design, planning and implementation’ of projects that will have an effect on them and from which they are going to benefit ; and that continuous feedback to communities forms a fundamental part of any development activity. This report explores how the “Breaking New Ground in Housing” (BNG) policy of the South African Government meets its objectives in the context of community participation.
- Full Text:
- Date Issued: 2011
Assessing the impact of integrated development planning (IDP) on gender equality and women's empowerment in South Africa: a case study of the Nelson Mandela Bay Metro Municipality
- Authors: Mpofu, Sibongile
- Date: 2011
- Subjects: Gender mainstreaming -- South Africa -- Nelson Mandela Bay Municipality , Women in development -- South Africa -- Nelson Mandela Bay Municipality , Equality -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4751 , vital:20672
- Description: The South African local government is expected to conform to all national policy directives including the principle of gender equality. Local government provides an immense opportunity for redressing imbalances and empowerment of women as they are the most negatively affected by poverty as a result of inequality. The South African Government has developed a wide range of legislation that ensures that women are empowered and participate in their own development. With all the legislation in place, women‟s empowerment as envisaged in the South African Constitution has, however, not yet been yielded the desired results as evidenced by the fact that women continue to remain disadvantaged in relation to men. This study therefore seeks to investigate whether and how the Nelson Mandela Bay Metropolitan Municipality (NMBMM) has mainstreamed gender in support to the principles of gender equality and women‟s empowerment in the implementation of Integrated Development Planning (IDP) with particular reference to wards 15 and 17. The researcher used a mixed methods research approach, this means that the study followed both the qualitative and quantitative approaches for in-depth understanding followed in the design and implementation of the IDP process. Data was collected from women participants by conducting surveys, making use of questionnaires. Structured interviews were conducted with municipality officials in order to give more insight more insight into how the IDP process is initiated in the Nelson Mandela Bay Metro Municipality. The findings of the study revealed that there is a lack of a strategic understanding of gender mainstreaming particularly by the leadership of the municipality. This as a result, has made operationalisation and engendering the IDP difficult. Due to this strategic vacuum, gender is generally not taken seriously when compared to other developmental challenges such as infrastructure provision. Moreover, the municipality does not have a coordination mechanism nor does it have a proper consultation mechanism to ensure that the nature of development projects is appropriate, effective and actually targets women‟s developmental needs. The leadership of the municipality must commit to the process of gender mainstreaming if the IDP is to be an effective tool in addressing the gender imbalances.
- Full Text:
- Date Issued: 2011
- Authors: Mpofu, Sibongile
- Date: 2011
- Subjects: Gender mainstreaming -- South Africa -- Nelson Mandela Bay Municipality , Women in development -- South Africa -- Nelson Mandela Bay Municipality , Equality -- South Africa -- Nelson Mandela Bay Municipality
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4751 , vital:20672
- Description: The South African local government is expected to conform to all national policy directives including the principle of gender equality. Local government provides an immense opportunity for redressing imbalances and empowerment of women as they are the most negatively affected by poverty as a result of inequality. The South African Government has developed a wide range of legislation that ensures that women are empowered and participate in their own development. With all the legislation in place, women‟s empowerment as envisaged in the South African Constitution has, however, not yet been yielded the desired results as evidenced by the fact that women continue to remain disadvantaged in relation to men. This study therefore seeks to investigate whether and how the Nelson Mandela Bay Metropolitan Municipality (NMBMM) has mainstreamed gender in support to the principles of gender equality and women‟s empowerment in the implementation of Integrated Development Planning (IDP) with particular reference to wards 15 and 17. The researcher used a mixed methods research approach, this means that the study followed both the qualitative and quantitative approaches for in-depth understanding followed in the design and implementation of the IDP process. Data was collected from women participants by conducting surveys, making use of questionnaires. Structured interviews were conducted with municipality officials in order to give more insight more insight into how the IDP process is initiated in the Nelson Mandela Bay Metro Municipality. The findings of the study revealed that there is a lack of a strategic understanding of gender mainstreaming particularly by the leadership of the municipality. This as a result, has made operationalisation and engendering the IDP difficult. Due to this strategic vacuum, gender is generally not taken seriously when compared to other developmental challenges such as infrastructure provision. Moreover, the municipality does not have a coordination mechanism nor does it have a proper consultation mechanism to ensure that the nature of development projects is appropriate, effective and actually targets women‟s developmental needs. The leadership of the municipality must commit to the process of gender mainstreaming if the IDP is to be an effective tool in addressing the gender imbalances.
- Full Text:
- Date Issued: 2011
Assessing the impact of school governance in the Limpopo Department of Education with specific reference to Mankweng and Polokwane circuits
- Authors: Mothapo, Sentshuhleng Jacob
- Date: 2011
- Subjects: School management and organization -- South Africa -- Limpopo , Leadership -- South Africa -- Limpopo , Responsibility -- South Africa -- Limpopo , Teacher effectiveness -- South Africa -- Limpopo , School boards -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Doctoral , PhD (in Public Administration)
- Identifier: vital:11663 , http://hdl.handle.net/10353/d1007096 , School management and organization -- South Africa -- Limpopo , Leadership -- South Africa -- Limpopo , Responsibility -- South Africa -- Limpopo , Teacher effectiveness -- South Africa -- Limpopo , School boards -- South Africa -- Limpopo
- Description: Education has been identified as a priority area by the South African government, in particular by the African National Congress as the ruling party. To this end, huge amounts of money are being spent on education as a service that has been approved by the legislators. Rules and regulations have been promulgated, and among others, the South African Schools Act, Act No. 84 of 1996, has been enacted. Rich research has also been funded with the sole intention of providing quality education to the people. Education of unacceptably poor quality has, however, been the result, as postulated by Peterson and Hassel (1998:55). The above are attested to by the findings that the political tensions emanating from the conduct of the South African Democratic Teachers’ Union and the Professional Educators’ Union, leading to class disruptions and general instability, erode the ethos of accountability on the part of educators and therefore impact negatively on service delivery. Furthermore, the Limpopo Department of Education is not immune to the challenges ranging from the elements of corrupt activities that often surface, teacher attrition which in the main is caused by lack of discipline. Winkler, Modise and Dawber (1998) indicate that teaching has never been easy, and many teachers are leaving their jobs because of the many problems with children in classrooms. Some of the problems cited are children who do not want to learn and learning that is becoming too difficult for the students because they do not want to listen. This study adopted sequential mixed methods namely, quantitative and qualitative research methods which are viewed as complementary rather than opposing approaches. Information was amassed from the subjects through interviews, observation, documentary survey and observation and the information has since been triangulated to validate the facts. All the methodologies employed proved to be useful in this study. The study sought to test the hypothesis “Good governance is informed by strong accountability and future-oriented organisation, continuously steering it towards its mission and vision, and thereby ensuring that the day-to-day management and administration are always linked with the organisation’s values and goals and thus eventually bringing about effectual and accelerated service delivery” to the South African populace without compromise. After empirically testing the hypothesis, showing mixed reaction informed by the findings of the study, five recommendations were made, based on the conclusions arrived at.
- Full Text:
- Date Issued: 2011
- Authors: Mothapo, Sentshuhleng Jacob
- Date: 2011
- Subjects: School management and organization -- South Africa -- Limpopo , Leadership -- South Africa -- Limpopo , Responsibility -- South Africa -- Limpopo , Teacher effectiveness -- South Africa -- Limpopo , School boards -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Doctoral , PhD (in Public Administration)
- Identifier: vital:11663 , http://hdl.handle.net/10353/d1007096 , School management and organization -- South Africa -- Limpopo , Leadership -- South Africa -- Limpopo , Responsibility -- South Africa -- Limpopo , Teacher effectiveness -- South Africa -- Limpopo , School boards -- South Africa -- Limpopo
- Description: Education has been identified as a priority area by the South African government, in particular by the African National Congress as the ruling party. To this end, huge amounts of money are being spent on education as a service that has been approved by the legislators. Rules and regulations have been promulgated, and among others, the South African Schools Act, Act No. 84 of 1996, has been enacted. Rich research has also been funded with the sole intention of providing quality education to the people. Education of unacceptably poor quality has, however, been the result, as postulated by Peterson and Hassel (1998:55). The above are attested to by the findings that the political tensions emanating from the conduct of the South African Democratic Teachers’ Union and the Professional Educators’ Union, leading to class disruptions and general instability, erode the ethos of accountability on the part of educators and therefore impact negatively on service delivery. Furthermore, the Limpopo Department of Education is not immune to the challenges ranging from the elements of corrupt activities that often surface, teacher attrition which in the main is caused by lack of discipline. Winkler, Modise and Dawber (1998) indicate that teaching has never been easy, and many teachers are leaving their jobs because of the many problems with children in classrooms. Some of the problems cited are children who do not want to learn and learning that is becoming too difficult for the students because they do not want to listen. This study adopted sequential mixed methods namely, quantitative and qualitative research methods which are viewed as complementary rather than opposing approaches. Information was amassed from the subjects through interviews, observation, documentary survey and observation and the information has since been triangulated to validate the facts. All the methodologies employed proved to be useful in this study. The study sought to test the hypothesis “Good governance is informed by strong accountability and future-oriented organisation, continuously steering it towards its mission and vision, and thereby ensuring that the day-to-day management and administration are always linked with the organisation’s values and goals and thus eventually bringing about effectual and accelerated service delivery” to the South African populace without compromise. After empirically testing the hypothesis, showing mixed reaction informed by the findings of the study, five recommendations were made, based on the conclusions arrived at.
- Full Text:
- Date Issued: 2011
Assessing the impact of unions and related barriers in lean manufacturing implementation within Eastern Cape automotive component suppliers
- Authors: Ebrahim, Zahier
- Date: 2011
- Subjects: Lean manufacturing , Motor industry -- South Africa -- Eastern Cape , Labor unions -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8791 , http://hdl.handle.net/10948/d1015690
- Description: Lean manufacturing provides a fundamental basis for competitiveness in the auto component sector. The implementation and sustainability of the lean philosophy are important aspects when deciding on the adoption of the lean concept within organisations. Various barriers to implementation present themselves during and after lean implementation. These barriers are often related and in some instances they may be converted from an impediment into an enabler of lean within companies. The impact of unions as a negative or positive influence forms a central focus of this research. The influences on the perception of unions and their constituents are studied in relation to other barriers in order to determine their relevance in the Eastern Cape auto sector. A research questionnaire was constructed in line with the selected research design. The application of triangulation in the research design allows for greater depth in the responses from the participants in this research. The quantitative analysis combined with the qualitative thematic analysis provides an excellent means to explore and understand these selected barriers to lean in great detail. It can be concluded that automotive trade unions in the Eastern Cape are a barrier to implementation; however the factor that influences lean the most is the incorrect perception of lean due to ineffective leadership practices among organisational and union leaders, among other barriers. The impact of unions on the acceptance of a lean culture has far-reaching implications for the auto sector that requires a specific implementation approach in order to change perceptions and gain the requisite interest alignment among unions and their constituents.
- Full Text:
- Date Issued: 2011
- Authors: Ebrahim, Zahier
- Date: 2011
- Subjects: Lean manufacturing , Motor industry -- South Africa -- Eastern Cape , Labor unions -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8791 , http://hdl.handle.net/10948/d1015690
- Description: Lean manufacturing provides a fundamental basis for competitiveness in the auto component sector. The implementation and sustainability of the lean philosophy are important aspects when deciding on the adoption of the lean concept within organisations. Various barriers to implementation present themselves during and after lean implementation. These barriers are often related and in some instances they may be converted from an impediment into an enabler of lean within companies. The impact of unions as a negative or positive influence forms a central focus of this research. The influences on the perception of unions and their constituents are studied in relation to other barriers in order to determine their relevance in the Eastern Cape auto sector. A research questionnaire was constructed in line with the selected research design. The application of triangulation in the research design allows for greater depth in the responses from the participants in this research. The quantitative analysis combined with the qualitative thematic analysis provides an excellent means to explore and understand these selected barriers to lean in great detail. It can be concluded that automotive trade unions in the Eastern Cape are a barrier to implementation; however the factor that influences lean the most is the incorrect perception of lean due to ineffective leadership practices among organisational and union leaders, among other barriers. The impact of unions on the acceptance of a lean culture has far-reaching implications for the auto sector that requires a specific implementation approach in order to change perceptions and gain the requisite interest alignment among unions and their constituents.
- Full Text:
- Date Issued: 2011
Assessing the statistical methodologies of business research in the South African context
- Authors: Ndou, Aifheli Amos
- Date: 2011
- Subjects: Statistics -- South Africa , Commercial statistics -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:8631 , http://hdl.handle.net/10948/1484 , Statistics -- South Africa , Commercial statistics -- South Africa
- Description: The aim of the study is to establish an acceptable classification scheme for the statistical methods used in business research. The approach compares the statistical component of the research and evaluates how it has changed over time and across different journals. If, as expected, the statistical expertise has changed, the change would be identified with the view to recommending curriculum changes for Statistics Departments of South African tertiary institutions.
- Full Text:
- Date Issued: 2011
- Authors: Ndou, Aifheli Amos
- Date: 2011
- Subjects: Statistics -- South Africa , Commercial statistics -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:8631 , http://hdl.handle.net/10948/1484 , Statistics -- South Africa , Commercial statistics -- South Africa
- Description: The aim of the study is to establish an acceptable classification scheme for the statistical methods used in business research. The approach compares the statistical component of the research and evaluates how it has changed over time and across different journals. If, as expected, the statistical expertise has changed, the change would be identified with the view to recommending curriculum changes for Statistics Departments of South African tertiary institutions.
- Full Text:
- Date Issued: 2011
Assessment of capacity development through public-private partnership: a case study of the Department of Economic Development and Environmental Affairs in the Eastern Cape
- Authors: Sogoni, Mbulelo
- Date: 2011
- Subjects: Human capital -- South Africa -- Eastern Cape , Public-private sector cooperation -- South Africa -- Eastern Cape , Economic development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11619 , http://hdl.handle.net/10353/327 , Human capital -- South Africa -- Eastern Cape , Public-private sector cooperation -- South Africa -- Eastern Cape , Economic development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape
- Description: The aim of this study was to examine the effectiveness of a capacity development programme initiated by the Department of Economic Development and Environmental Affairs (DEDEA) as a mechanism to enhance the performance of the employees in order to improve service delivery to the people of the Eastern Cape. The public service is enjoined by the Constitution to provide services that citizens need, and for this reason it is in need of educated, trained and professional public servants, especially in the technical, professional and managerial categories. The department also recognized the potential of public–private partnerships (PPPs) to improve performance in the areas of transformation and service delivery, and that it required investments aimed at developing and strengthening public management capacity. However, the effectiveness of the PPP as a mechanism for capacity development, remained unknown. The literature reviewed overwhelmingly supported the assertion that human capital development is critical for performance in the public service. The review also highlighted the importance of partnerships, especially the role of the private sector in the area of capacity development, advancing the argument that PPPs should be pursued in order to leverage ideas, resources, and capabilities to achieve public service goals. Various research tools, namely interviews, questionnaires and document analysis were used to conduct the study. Data collected and analysed provided empirical evidence of improved performance of both the individual managers and the department in the functional areas of financial governance and management; programme and project management; as well as ethics and professionalism. It suggested a strong correlation between training, capacity development and service delivery. This research found the DEDEA/National Business Initiative (NBI) PPP initiative, as a capacity development mechanism to enhance the performance of the department‟s employees, effective. On strength of the overwhelming evidence gathered through this research, it strongly recommends that the programme be rolled out to other employees.
- Full Text:
- Date Issued: 2011
- Authors: Sogoni, Mbulelo
- Date: 2011
- Subjects: Human capital -- South Africa -- Eastern Cape , Public-private sector cooperation -- South Africa -- Eastern Cape , Economic development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: vital:11619 , http://hdl.handle.net/10353/327 , Human capital -- South Africa -- Eastern Cape , Public-private sector cooperation -- South Africa -- Eastern Cape , Economic development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape
- Description: The aim of this study was to examine the effectiveness of a capacity development programme initiated by the Department of Economic Development and Environmental Affairs (DEDEA) as a mechanism to enhance the performance of the employees in order to improve service delivery to the people of the Eastern Cape. The public service is enjoined by the Constitution to provide services that citizens need, and for this reason it is in need of educated, trained and professional public servants, especially in the technical, professional and managerial categories. The department also recognized the potential of public–private partnerships (PPPs) to improve performance in the areas of transformation and service delivery, and that it required investments aimed at developing and strengthening public management capacity. However, the effectiveness of the PPP as a mechanism for capacity development, remained unknown. The literature reviewed overwhelmingly supported the assertion that human capital development is critical for performance in the public service. The review also highlighted the importance of partnerships, especially the role of the private sector in the area of capacity development, advancing the argument that PPPs should be pursued in order to leverage ideas, resources, and capabilities to achieve public service goals. Various research tools, namely interviews, questionnaires and document analysis were used to conduct the study. Data collected and analysed provided empirical evidence of improved performance of both the individual managers and the department in the functional areas of financial governance and management; programme and project management; as well as ethics and professionalism. It suggested a strong correlation between training, capacity development and service delivery. This research found the DEDEA/National Business Initiative (NBI) PPP initiative, as a capacity development mechanism to enhance the performance of the department‟s employees, effective. On strength of the overwhelming evidence gathered through this research, it strongly recommends that the programme be rolled out to other employees.
- Full Text:
- Date Issued: 2011
Assessment of organochlorine pesticide residues in fish samples from the Okavango Delta, Botswana
- Authors: Mpofu, Christopher
- Date: 2011 , 2011-02-28
- Subjects: Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4404 , http://hdl.handle.net/10962/d1006707 , Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Description: This thesis presents an evaluation of the dispersive solid-phase extraction (d-SPE) method referred to as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for the determination of four organochlorine pesticide residues in fish samples. The pesticides investigated in this study were o, p′-DDT, p, p′-DDE, aldrin and dieldrin. The combined use of Gas Chromatography with an Electron Capture Detector (GC-ECD) and sensitive Time of Flight (TOF) mass detector facilitated the identification of the target analytes. In the absence of certified reference material, the overall analytical procedure was validated by systematic recovery experiments on spiked samples at three levels of 2, 5 and 10 ng/g. The targeted compounds were successfully extracted and their recovery ranged from 76 to 96% with relative standard deviations of less than 13%. The optimum QuEChERS conditions were 2g of fish powder, 10 ml acetonitrile and 1 min shaking time. The optimal conditions were applied to assess the levels of chlorinated pesticides in blunt-tooth catfish (Clarias ngamensis), tigerfish (Hydrocynus vittatus), Oreochromis andersonii and red-breasted tilapia (Tilapia rendalli) from the Okavango Delta, Botswana. Dieldrin, p, p′-DDE and aldrin were detected in all the analysed samples with a concentration range of 0.04 – 0.29, 0.07 – 0.33, 0.04 – 0.28 and 0.03 – 0.24 ng/g per dry weight in O. andersonii, C. ngamensis, T. rendalli and H. vittatus respectively. These concentrations were below the US-EPA 0.1 μg/g allowable limit in edible fish and the Australian Maximum Residue Limit (MRL) of 50 - 1000 ng/g in fresh fish. DDT was not detected in all the fish species investigated. The mean lipid content recorded in the fish samples were 1.24, 2.16, 2.18 and 4.21% for H. vittatus, T. rendalli, O. andersonni and C. ngamensis respectively. No systematic trend was observed between fish age and pesticide levels in fish. Acetylcholinesterase (AChE) activity assays were performed to assess the effects of organochlorine pesticides in Clarias ngamensis. The enzyme activity recorded in Clarias ngamensis from the Okavango Delta and the reference site was 12.31 μmol of acetylcholine iodide hydrolysed/min/g brain tissue. The enzyme activity remained the same indicating no enzyme inhibition. The conclusions drawn from this study are that the QuEChERS method is applicable for the determination of organochlorine pesticide residues in fish matrices. The fish from the Okavango Delta are safe for human consumption.
- Full Text:
- Date Issued: 2011
- Authors: Mpofu, Christopher
- Date: 2011 , 2011-02-28
- Subjects: Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4404 , http://hdl.handle.net/10962/d1006707 , Pesticides , Organochlorine compounds , Freshwater fishes -- Effect of pesticides on -- Botswana -- Okavango River Delta , Water -- Pesticide content -- Botswana -- Okavango River Delta , Catfishes -- Botswana -- Okavango River Delta , Tigerfish -- Botswana -- Okavango River Delta , Redbreast tilapia -- Botswana -- Okavango River Delta
- Description: This thesis presents an evaluation of the dispersive solid-phase extraction (d-SPE) method referred to as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for the determination of four organochlorine pesticide residues in fish samples. The pesticides investigated in this study were o, p′-DDT, p, p′-DDE, aldrin and dieldrin. The combined use of Gas Chromatography with an Electron Capture Detector (GC-ECD) and sensitive Time of Flight (TOF) mass detector facilitated the identification of the target analytes. In the absence of certified reference material, the overall analytical procedure was validated by systematic recovery experiments on spiked samples at three levels of 2, 5 and 10 ng/g. The targeted compounds were successfully extracted and their recovery ranged from 76 to 96% with relative standard deviations of less than 13%. The optimum QuEChERS conditions were 2g of fish powder, 10 ml acetonitrile and 1 min shaking time. The optimal conditions were applied to assess the levels of chlorinated pesticides in blunt-tooth catfish (Clarias ngamensis), tigerfish (Hydrocynus vittatus), Oreochromis andersonii and red-breasted tilapia (Tilapia rendalli) from the Okavango Delta, Botswana. Dieldrin, p, p′-DDE and aldrin were detected in all the analysed samples with a concentration range of 0.04 – 0.29, 0.07 – 0.33, 0.04 – 0.28 and 0.03 – 0.24 ng/g per dry weight in O. andersonii, C. ngamensis, T. rendalli and H. vittatus respectively. These concentrations were below the US-EPA 0.1 μg/g allowable limit in edible fish and the Australian Maximum Residue Limit (MRL) of 50 - 1000 ng/g in fresh fish. DDT was not detected in all the fish species investigated. The mean lipid content recorded in the fish samples were 1.24, 2.16, 2.18 and 4.21% for H. vittatus, T. rendalli, O. andersonni and C. ngamensis respectively. No systematic trend was observed between fish age and pesticide levels in fish. Acetylcholinesterase (AChE) activity assays were performed to assess the effects of organochlorine pesticides in Clarias ngamensis. The enzyme activity recorded in Clarias ngamensis from the Okavango Delta and the reference site was 12.31 μmol of acetylcholine iodide hydrolysed/min/g brain tissue. The enzyme activity remained the same indicating no enzyme inhibition. The conclusions drawn from this study are that the QuEChERS method is applicable for the determination of organochlorine pesticide residues in fish matrices. The fish from the Okavango Delta are safe for human consumption.
- Full Text:
- Date Issued: 2011
Assessment of the antibacterial properties of n-Hexane extract of Cocos Nucifera and its interactions with some conventional antibiotics
- Authors: Akinyele, Taiwo Adesola
- Date: 2011
- Subjects: Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11245 , http://hdl.handle.net/10353/416 , Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Description: Cocos nucifera belong to the family Aracaceae (palm Family). The English name is coconut and it is used extensively as medicinal remedies against infections such as urinary tract infections, gastro intestinal infections, skin and wound infections. The in vitro antibacterial (including anti-listerial and anti-vibrio) properties as well as the evaluation of the combination potentials of the plant extract with six front-line antibiotics were evaluated in this study using standard procedures. The in vitro anti-listerial properties of the crude aqueous and n-Hexane extract of the husk of Cocos nucifera were carried out against 37 Listeria isolates. Twenty-nine of the test organisms were susceptible to the aqueous extract while thirty were susceptible to the n-Hexane extract both at the screening concentration of 25 mg/ml. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.6 - 5.0 mg/ml. For the aqueous extract, average log reduction in viable cell count ranged between 0.32 Log10 and 4.8 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 2.4 Log10 and 6.2 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. The time-kill characteristics of the two extracts suggest that at higher concentration (2 × MIC) and longer duration of interaction (8 hr), more bacteria were killed. In vitro anti-vibrio and antibacterial properties experiment revealed that of all the 45 vibrio and 25 bacteria strains that was tested, 37 were susceptible to the aqueous extract and 38 to the n-Hexane extract, while 17 were susceptible to the aqueous extract and 21 to the n-Hexane extract. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.3 - 5.0 mg/ml. viii The time kill studies revealed that for the aqueous extract, average log reduction in viable cell count in time kill assay ranged between 0.12 Log10 and 4.2 Log10 CFU/ml after 8 hr interaction at 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 0.56 Log10 and 6.4 Log10 CFU/ml after 8 hr interaction in 1 × MIC and 2 × MIC. In the test for the combination interactions, the checkerboard method revealed synergy of 67% and indifferent of 33%, while the time kill assay detected synergy in 72% and indifferent in 28% of the combinations tested. The synergy detected was not specific to any of the antibiotics or the Gram reaction of the bacteria, and no antagonism was detected. We conclude that the aqueous and n-Hexane extract of the husk of C. nucifera contains potential broad spectrum antibiotics resistance modulating compounds that could be relevant in the treatment of infections caused by these pathogens. In addition, the husk which is being discarded as agro waste will opens up a vista of opportunities for utilization for therapeutic purposes
- Full Text:
- Date Issued: 2011
- Authors: Akinyele, Taiwo Adesola
- Date: 2011
- Subjects: Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11245 , http://hdl.handle.net/10353/416 , Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Description: Cocos nucifera belong to the family Aracaceae (palm Family). The English name is coconut and it is used extensively as medicinal remedies against infections such as urinary tract infections, gastro intestinal infections, skin and wound infections. The in vitro antibacterial (including anti-listerial and anti-vibrio) properties as well as the evaluation of the combination potentials of the plant extract with six front-line antibiotics were evaluated in this study using standard procedures. The in vitro anti-listerial properties of the crude aqueous and n-Hexane extract of the husk of Cocos nucifera were carried out against 37 Listeria isolates. Twenty-nine of the test organisms were susceptible to the aqueous extract while thirty were susceptible to the n-Hexane extract both at the screening concentration of 25 mg/ml. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.6 - 5.0 mg/ml. For the aqueous extract, average log reduction in viable cell count ranged between 0.32 Log10 and 4.8 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 2.4 Log10 and 6.2 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. The time-kill characteristics of the two extracts suggest that at higher concentration (2 × MIC) and longer duration of interaction (8 hr), more bacteria were killed. In vitro anti-vibrio and antibacterial properties experiment revealed that of all the 45 vibrio and 25 bacteria strains that was tested, 37 were susceptible to the aqueous extract and 38 to the n-Hexane extract, while 17 were susceptible to the aqueous extract and 21 to the n-Hexane extract. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.3 - 5.0 mg/ml. viii The time kill studies revealed that for the aqueous extract, average log reduction in viable cell count in time kill assay ranged between 0.12 Log10 and 4.2 Log10 CFU/ml after 8 hr interaction at 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 0.56 Log10 and 6.4 Log10 CFU/ml after 8 hr interaction in 1 × MIC and 2 × MIC. In the test for the combination interactions, the checkerboard method revealed synergy of 67% and indifferent of 33%, while the time kill assay detected synergy in 72% and indifferent in 28% of the combinations tested. The synergy detected was not specific to any of the antibiotics or the Gram reaction of the bacteria, and no antagonism was detected. We conclude that the aqueous and n-Hexane extract of the husk of C. nucifera contains potential broad spectrum antibiotics resistance modulating compounds that could be relevant in the treatment of infections caused by these pathogens. In addition, the husk which is being discarded as agro waste will opens up a vista of opportunities for utilization for therapeutic purposes
- Full Text:
- Date Issued: 2011