Cultivation, nutritional and pharmacological evaluation of Celosia argentea (L) Kuntze: an endangered wild vegetable in the Eastern Cape Province of South Africa
- Adegbaju, Oluwafunmilayo Dorcas
- Authors: Adegbaju, Oluwafunmilayo Dorcas
- Date: 2019-09
- Subjects: Celosia , Edible greens
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19806 , vital:43250
- Description: Green leafy vegetables are important in human nutrition, but their cultivation is limited to a few staple ones. These vegetables possess high nutritious and therapeutic properties that could help in achieving nutritional security and alleviating the burden of some diseases. A typical example is Celosia argentea, which in South Africa, is little known and grossly under-utilized despite its numerous nutritional and pharmacological values. The present study investigated the cultivation, nutritional and pharmacological potentials of C. argentea at three stages of maturity of two trials. Evaluation of cultivation in the greenhouse towards possible domestication of C. argentea included seed viability test, seed germination under varying temperatures (5, 10, 15, 20 25 30, 35, 40°C), light conditions (continuous light, continuous darkness, alternating light and dark (12h light/12 dark photoperiod) and sowing depths (0, 1, 2, 3, 4 and 5cm). The nutritional evaluation assessed the proximal, mineral, vitamins and antinutrient content of the plant; while pharmacological potentials of C. argentea were evaluated by investigating the ultramorphology, phytochemical content, anti-oxidant, antimicrobial, toxicity and cell-based anti-inflammatory properties of the plant at different stages of growth. Germination experiment revealed that optimum seed germination requirements for C. argentea were 25°C, alternating light and dark regime and a sowing depth of 1cm. Microrphological assessment of C. argentea revealed that the leaves were amphistomatic; with the abaxial surface having higher stomata density. Characteristic, distinguishing protuberances were observed at the polar ends of the stomata on the adaxial surface of the plant epidermis, and the major elements on the foliar epidermis were beryllium, carbon, oxygen and potassium; while pigmentation and colouration, showing localization of bioactive compounds were mostly at the site where trichomes were abundant. Growth parameters and yield potential (plant height, stem girth, leaf area, number of leaves, number of branches, number of days to flowering, number of flowers as well as dry and fresh weight) of C. argentea showed that for agricultural practices on the field, increase in growth parameters should be expected around 4-5 weeks after transplanting while higher yield should be expected around 7-9 weeks after transplanting. The results encouraged cultivation during summer or late spring for best farm management. Proximal, mineral, vitamins and anti-nutrients content of C. argentea at three different maturity stages of two trials were performed using AOAC, ALASA and inductively coupled plasma-optical emission spectrometer techniques. Results revealed that the pre-flowering stage of growth had the highest ash (28.15± 0.10 percent) and crude protein (25.80 ± 0.20 percent) contents. While post-flowering stage had the highest carbohydrate (28.51± 0.20 percent and 36.16± 0.22 percent), crude fibre (33.41± 0.87 percent) and energy (435.28± 27.6 percent) with low fat and moisture (8.43± 0.15 percent and 6.35±0.09 percent) contents respectively; while no marked demarcation in most mineral contents in all the growth stages was observed. Zinc was highest at the flowering stage of growth, while vitamin contents decreased as the plant approached maturation. Antinutrients content of the plant was not dependent on growth stage. Total phenols, flavonoids and proanthocyanidin content of C. argentea were evaluated colourimetrically, while the antioxidant potency of the aqueous, acetone and methanolic extracts was measured using 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) models. All the solvent extracts of the flowering stage had the highest total phenolic (80.75±4.21GAE/g), flavonoids (946.19±7.87QE/mg) and proanthocyanidin contents (100.90±1.29 CE/g); with the acetone extracts showing significantly higher phytochemical content. The flowering stage exhibited the best radical inhibitory activity, with the methanol extract having the highest scavenging power for ABTS and DPPH radicals, while acetone extract showed the highest inhibition against FRAP and highest total antioxidant capacity. The antimicrobial potential of the aqueous acetone and methanolic extracts of C. argentea evaluated using agar dilution method against six bacteria (Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus) and four fungal strains (Candida glabarata, Candida albicans and Penicillium aurantiogriseum and Penicillium chrysogenum) showed that Streptococcus pyrogenes and P. aeruginosa were susceptible to the methanol extracts of all the growing phases of both trials at 10 mg/mL; while Klebsiella pneumoniae was susceptible at 10 mg/mL to only the methanol post-flowering extracts of both trials. C. albicans and P. aurantiogriseum were highly susceptible to all the extracts. Possible toxicity evaluated using brine shrimp lethality assay and calculation of LC50 against brine shrimp nauplii revealed that C. argentea was not toxic at any stage of maturity at all concentrations evaluated. Anti-inflammatory activities and cytotoxicity of C. argentea extracts evaluated using RAW 264.7 macrophages and 3T3-L1 cell lines revealed that the acetone extract of the flowering stage had moderate anti-inflammatory activity with no significant toxicity against activated macrophages. Findings from this study indicate that C. argentea is a safe functional leafy vegetable of high nutritional and pharmacological importance which can easily be cultivated and domesticated in South Africa. This could significantly alleviate the burden of micronutrient deficiencies among pre-school children in rural communities. , Thesis (PhD) (Botany) -- University of Fort Hare, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Adegbaju, Oluwafunmilayo Dorcas
- Date: 2019-09
- Subjects: Celosia , Edible greens
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19806 , vital:43250
- Description: Green leafy vegetables are important in human nutrition, but their cultivation is limited to a few staple ones. These vegetables possess high nutritious and therapeutic properties that could help in achieving nutritional security and alleviating the burden of some diseases. A typical example is Celosia argentea, which in South Africa, is little known and grossly under-utilized despite its numerous nutritional and pharmacological values. The present study investigated the cultivation, nutritional and pharmacological potentials of C. argentea at three stages of maturity of two trials. Evaluation of cultivation in the greenhouse towards possible domestication of C. argentea included seed viability test, seed germination under varying temperatures (5, 10, 15, 20 25 30, 35, 40°C), light conditions (continuous light, continuous darkness, alternating light and dark (12h light/12 dark photoperiod) and sowing depths (0, 1, 2, 3, 4 and 5cm). The nutritional evaluation assessed the proximal, mineral, vitamins and antinutrient content of the plant; while pharmacological potentials of C. argentea were evaluated by investigating the ultramorphology, phytochemical content, anti-oxidant, antimicrobial, toxicity and cell-based anti-inflammatory properties of the plant at different stages of growth. Germination experiment revealed that optimum seed germination requirements for C. argentea were 25°C, alternating light and dark regime and a sowing depth of 1cm. Microrphological assessment of C. argentea revealed that the leaves were amphistomatic; with the abaxial surface having higher stomata density. Characteristic, distinguishing protuberances were observed at the polar ends of the stomata on the adaxial surface of the plant epidermis, and the major elements on the foliar epidermis were beryllium, carbon, oxygen and potassium; while pigmentation and colouration, showing localization of bioactive compounds were mostly at the site where trichomes were abundant. Growth parameters and yield potential (plant height, stem girth, leaf area, number of leaves, number of branches, number of days to flowering, number of flowers as well as dry and fresh weight) of C. argentea showed that for agricultural practices on the field, increase in growth parameters should be expected around 4-5 weeks after transplanting while higher yield should be expected around 7-9 weeks after transplanting. The results encouraged cultivation during summer or late spring for best farm management. Proximal, mineral, vitamins and anti-nutrients content of C. argentea at three different maturity stages of two trials were performed using AOAC, ALASA and inductively coupled plasma-optical emission spectrometer techniques. Results revealed that the pre-flowering stage of growth had the highest ash (28.15± 0.10 percent) and crude protein (25.80 ± 0.20 percent) contents. While post-flowering stage had the highest carbohydrate (28.51± 0.20 percent and 36.16± 0.22 percent), crude fibre (33.41± 0.87 percent) and energy (435.28± 27.6 percent) with low fat and moisture (8.43± 0.15 percent and 6.35±0.09 percent) contents respectively; while no marked demarcation in most mineral contents in all the growth stages was observed. Zinc was highest at the flowering stage of growth, while vitamin contents decreased as the plant approached maturation. Antinutrients content of the plant was not dependent on growth stage. Total phenols, flavonoids and proanthocyanidin content of C. argentea were evaluated colourimetrically, while the antioxidant potency of the aqueous, acetone and methanolic extracts was measured using 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) models. All the solvent extracts of the flowering stage had the highest total phenolic (80.75±4.21GAE/g), flavonoids (946.19±7.87QE/mg) and proanthocyanidin contents (100.90±1.29 CE/g); with the acetone extracts showing significantly higher phytochemical content. The flowering stage exhibited the best radical inhibitory activity, with the methanol extract having the highest scavenging power for ABTS and DPPH radicals, while acetone extract showed the highest inhibition against FRAP and highest total antioxidant capacity. The antimicrobial potential of the aqueous acetone and methanolic extracts of C. argentea evaluated using agar dilution method against six bacteria (Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus) and four fungal strains (Candida glabarata, Candida albicans and Penicillium aurantiogriseum and Penicillium chrysogenum) showed that Streptococcus pyrogenes and P. aeruginosa were susceptible to the methanol extracts of all the growing phases of both trials at 10 mg/mL; while Klebsiella pneumoniae was susceptible at 10 mg/mL to only the methanol post-flowering extracts of both trials. C. albicans and P. aurantiogriseum were highly susceptible to all the extracts. Possible toxicity evaluated using brine shrimp lethality assay and calculation of LC50 against brine shrimp nauplii revealed that C. argentea was not toxic at any stage of maturity at all concentrations evaluated. Anti-inflammatory activities and cytotoxicity of C. argentea extracts evaluated using RAW 264.7 macrophages and 3T3-L1 cell lines revealed that the acetone extract of the flowering stage had moderate anti-inflammatory activity with no significant toxicity against activated macrophages. Findings from this study indicate that C. argentea is a safe functional leafy vegetable of high nutritional and pharmacological importance which can easily be cultivated and domesticated in South Africa. This could significantly alleviate the burden of micronutrient deficiencies among pre-school children in rural communities. , Thesis (PhD) (Botany) -- University of Fort Hare, 2019
- Full Text:
- Date Issued: 2019-09
Parents’ and teachers’ perspectives towards teaching and learning of intellectually impaired learners at the Foundation Phase in Mthatha Education Sub-District
- Authors: Magwenshu, Tulile Angela
- Date: 2019-09
- Subjects: Inclusive education
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/6604 , vital:47157
- Description: The purpose of this study was to investigate parents’ and teachers’ perspectives towards the teaching and learning of intellectually impaired learners at Foundation Phase in Mthatha Education Sub-District. This study explored the lived experiences of parents and teachers of intellectually impaired learners in this special school. It determined parents’ and teachers’ understanding of intellectual disability, the challenges they face in working with such children and strategies they use to deal with intellectually challenged learners. The research was limited to one special school in Mthatha, which is the only special school in this sub-district. The study used the qualitative research approach in the form of case study research design. The sample consisted of twelve participants, four parents of learners who are intellectually impaired and eight Foundation Phase teachers in this special school. These are some of the findings: The Education White Paper 6 on building an inclusive education and training is effective even though it is moving slowly. There has been no preparation on how it should be implemented in terms of human and other resources, knowledge and skills on special needs education. The challenges teachers face in teaching learners who are intellectually impaired include: learner-teacher ratio that is not followed, hence overcrowded classes; classroom management; lack of learner support materials; no special curriculum designed for intellectually impaired learners; confining subject matter and teachers not participating in skills development. The mechanism that were suggested to assist learners who are intellectually impaired included: equipping teachers with skills necessary for learning and teaching of intellectually impaired learners, establishing institutional level support teams, making more learning and teaching resources available, providing qualified human resources, mobilizing public support, government understanding impairment and therapists being employed (psychologists, physicians, nurses, doctors and social workers). Some of the recommendations are: conducting workshops on effective coping strategies for teachers, public awareness campaigns in communities about intellectual disability, school governing bodies working with the government to supply the school with adequate learner support materials, proper infrastructure, School Based Support Teams support service, the District taking responsibility for establishing networks that promote effective communication between learners, teachers and parents as well as with non-governmental organizations and welfare, health and justice departments and Supporting teachers on site. , Thesis (M.Ed) -- Faculty of Education Sciences, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Magwenshu, Tulile Angela
- Date: 2019-09
- Subjects: Inclusive education
- Language: English
- Type: Master's/Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/6604 , vital:47157
- Description: The purpose of this study was to investigate parents’ and teachers’ perspectives towards the teaching and learning of intellectually impaired learners at Foundation Phase in Mthatha Education Sub-District. This study explored the lived experiences of parents and teachers of intellectually impaired learners in this special school. It determined parents’ and teachers’ understanding of intellectual disability, the challenges they face in working with such children and strategies they use to deal with intellectually challenged learners. The research was limited to one special school in Mthatha, which is the only special school in this sub-district. The study used the qualitative research approach in the form of case study research design. The sample consisted of twelve participants, four parents of learners who are intellectually impaired and eight Foundation Phase teachers in this special school. These are some of the findings: The Education White Paper 6 on building an inclusive education and training is effective even though it is moving slowly. There has been no preparation on how it should be implemented in terms of human and other resources, knowledge and skills on special needs education. The challenges teachers face in teaching learners who are intellectually impaired include: learner-teacher ratio that is not followed, hence overcrowded classes; classroom management; lack of learner support materials; no special curriculum designed for intellectually impaired learners; confining subject matter and teachers not participating in skills development. The mechanism that were suggested to assist learners who are intellectually impaired included: equipping teachers with skills necessary for learning and teaching of intellectually impaired learners, establishing institutional level support teams, making more learning and teaching resources available, providing qualified human resources, mobilizing public support, government understanding impairment and therapists being employed (psychologists, physicians, nurses, doctors and social workers). Some of the recommendations are: conducting workshops on effective coping strategies for teachers, public awareness campaigns in communities about intellectual disability, school governing bodies working with the government to supply the school with adequate learner support materials, proper infrastructure, School Based Support Teams support service, the District taking responsibility for establishing networks that promote effective communication between learners, teachers and parents as well as with non-governmental organizations and welfare, health and justice departments and Supporting teachers on site. , Thesis (M.Ed) -- Faculty of Education Sciences, 2019
- Full Text:
- Date Issued: 2019-09
Studies on vine storage methods, planting techniques, paclobutrazol treatment, planting density and weevil management on sweetpotato (ipomoea batatas l.lam) cultivars promoted in South Africa
- Hlerema, Igenicious Nicholas https://orcid.org/0000-0002-6111-404X
- Authors: Hlerema, Igenicious Nicholas https://orcid.org/0000-0002-6111-404X
- Date: 2019-09
- Subjects: Sweet potatoes , Seed treatment
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21580 , vital:49336
- Description: Sweetpotato (Ipomoea batatas L. LAM) is a major world staple food. It is a versatile, nutrient-rich crop well suited for production by emerging farmers, and thus a popular crop in subtropical parts of South Africa. The crop has several advantages such as producing more bioweight and nutrients per unit land area and per unit time than most other staple crops such as maize; adaptation to a broad range of agro-ecological conditions; and ability to compete and crowd out weeds, and affected by a relatively small number of pests and diseases. Root and vine yields achieved by emerging farmers are low and cultivation systems need to be optimized. The general objective of the research was to develop nursery practices that ensure a sustainable supply of sweetpotato planting materials and cultural practices that maximize yield, eating and nutritional quality of sweetpotato roots. The specific objectives were (i) to evaluate the effect of polyethylene and hessian bags on vine quality and vine survival period; (ii) to compare planting techniques and vine length; (iii) to determine the effect of paclobutrazol on storage root yield; and (iv) to evaluate the effect of Beauveria bassiana as biological control for sweetpotato weevil. The vine storage experiment aimed at identifying the best type of storage bags that keep the vines of “Bophelo”, an orange-fleshed sweetpotato cultivar in good quality for a longer period of time. The study involved two factors, viz storage bag type and storage period. The experiment tried to address (1) a sustainable seed system in sweetpotato and (2) production stimulated by cultural practises. Vines were stored in polyethylene bag without holes, polyethylene bag with 12 holes and dry hessian bag over 3, 6, 9, 12 and 15 days at an ambient temperature of 250C. The incremental cost-effectiveness ratio (ICER) was calculated to determine the cost-effectiveness of using the polyethylene bags with holes or without and the hessian bags. The results of the study showed that hessian bags store vines over a longer period (12 days) compared to the polyethylene bags without holes (6 days); however, hessian bags are more expensive. Polyethylene bags can still be used for transporting and storing vines if the farmer is within a short distance from the seed source and plants the vines within the 6 day period. Polyethylene bags with 12 holes are recommended since they allow maximum air circulation and extend storage period (9 days). Optimum planting techniques can help sweetpotato farmers to achieve optimal yields. Newly developed cultivars from the Agricultural Research Council have not been tested to determine the best planting techniques. A trial was conducted to investigate the effect of two planting orientations (horizontal and vertical) and different vine length (2, 3 and 4 subterranean nodes) of 3 released varieties, Blesbock, Bophelo and Ndou on storage root yield of sweetpotato. A factorial experiment was laid out in a randomised complete block design with three replications during two cropping seasons (2014/15 and 2015/16). The data collected in both experiments included total storage root yield, marketable storage root yield, and number of storage roots per size class. Significant differences were detected for the interaction of all factors on the number roots per size class, total storage root yield, and total average root weight. Vertical and horizontal planting per se showed no significance differences for all the sweetpotato root growth parameters such as total yield. However, the more the number of subterainian node the more yield we got for the commercial cultivar Blesbok. Paclobutrazol (PBZ) is a plant growth retardant hormone. This hormone might, therefore, be useful for sweetpotato growers to achieve higher plant populations per hectare because it would reduce the plant canopy. However, it should also be checked if the PBZ application does not affect nutrient content of roots and leaves, especially the beta-carotene content of the orange-fleshed cultivars. The objective of this study was, therefore, to determine the effect of PBZ on sweetpotato shoot growth, storage root yield and beta-carotene content of sweetpotato leaves and storage roots. The field trial was repeated over two seasons, namely 2014/15 and 2015/16 crop seasons. These field experiments were arranged as a split plot experiment. The main plots were allocated to four PBZ levels, laid-out as randomised complete block design, and treatment combinations of two cultivars (Bophelo and Blesbok) and two plants spacing (20 cm and 30 cm) were randomly allocated into the subplots. The PBZ levels applied were 0, 100, 200 and 300 mg of active ingredient per litre of water, sprayed 4 weeks after planting. Root yield and bioweight accumulation were recorded. In addition, a pot experiment was set up using cultivar Bophelo and 0 mg, 100 mg and 200 mg/l active ingredient of PBZ sprayed 4 weeks after planting. Leaf and root samples (150g and 400g of a composite sample respectively) were collected and analysed for total phenols, total flavonoids, vitamin C and beta-carotene. The field trial results revealed significant differences (P<0.05) in leaf and stem weight of the plants treated with different PBZ levels applied. The control (0 mg PBZ rate) had the highest leaf and stem weight. There were no significant differences in root yield among the PBZ levels. The results of leaf and root analysis for the Bophelo cultivar showed significant differences (P<0.05) in total phenols, total flavonoids, vitamin C and beta-carotene in leaf samples of the three levels of PBZ applied. The beta-carotene for the leaf increased at the 100 mg/l PBZ level. As the paclobutrazol level increased there was a reduction in the beta-carotene content for the roots from 18,91 to 13,97 to 10,77 mg/100g dry weight for 0 mg/l, 100 mg/l and 200mg/l, respectively. Application of PBZ to sweetpotato reduces the bioweight yield but does not have an effect on the root yield. Thus a higher plant density can be used to achieve higher yield and returns on investment. The leaves can be used as a relish and can be sold to supplement income. However, PBZ further reduces the beta-carotene content of the roots and is not recommended for use with orange-fleshed cultivars, where the objective is to enrich food with vitamin A. Sweetpotato weevils (Cylas puncticollis and C. formicarius) are notorious pests of sweetpotato. It causes severe losses in marketable root yield. The study tested the use of Beauveria bassiana (Balsamo Vuillemin) (Deuteromycota: Hyphomycetes) for controlling sweetpotato weevils. Experiments were conducted over two cropping seasons using four treatments: 1) dipping sweetpotato cuttings in solution of 1 g/litre B. bassiana before planting, 2) spraying with a registered chemical (Deltametrin) at 50 ml/100 litre of water starting 2 weeks after panting at biweekly basis for four months after planting, 3) spraying the leaves with B. bassiana (10 g/100 litre) starting 2 weeks on biweekly basis for four months after planting; and 4) control (sprayed with water). The research was arranged as split plot experiment, where the pest management treatments assigned to the main plots as per Latin square design and the three sweetpotato cultivars (Bophelo, Ndou and Blesbok) were randomly put in the subplot in each main plot. Spraying with B. bassiana significantly reduced the percentage of insect-damaged roots (9.95percent) compared to the control (22.48percent). Dipping sweetpotato cuttings in solution of 1 g/litre B. bassiana before planting did not significantly reduce the number of insect damaged roots (18.63percent insect damage). There was no significant difference between the chemical spray (8.2percent insect damage) and spraying with B. bassiana (9.95percent insect damage). The investigation indicated that B. bassiana can be considered as an alternative control method for the sweetpotato weevil. Use of biological control is less expensive and beneficial to reduce adverse effects on the environment and human health. , Thesis (PhD) -- Faculty of Science and Agriculture, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Hlerema, Igenicious Nicholas https://orcid.org/0000-0002-6111-404X
- Date: 2019-09
- Subjects: Sweet potatoes , Seed treatment
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21580 , vital:49336
- Description: Sweetpotato (Ipomoea batatas L. LAM) is a major world staple food. It is a versatile, nutrient-rich crop well suited for production by emerging farmers, and thus a popular crop in subtropical parts of South Africa. The crop has several advantages such as producing more bioweight and nutrients per unit land area and per unit time than most other staple crops such as maize; adaptation to a broad range of agro-ecological conditions; and ability to compete and crowd out weeds, and affected by a relatively small number of pests and diseases. Root and vine yields achieved by emerging farmers are low and cultivation systems need to be optimized. The general objective of the research was to develop nursery practices that ensure a sustainable supply of sweetpotato planting materials and cultural practices that maximize yield, eating and nutritional quality of sweetpotato roots. The specific objectives were (i) to evaluate the effect of polyethylene and hessian bags on vine quality and vine survival period; (ii) to compare planting techniques and vine length; (iii) to determine the effect of paclobutrazol on storage root yield; and (iv) to evaluate the effect of Beauveria bassiana as biological control for sweetpotato weevil. The vine storage experiment aimed at identifying the best type of storage bags that keep the vines of “Bophelo”, an orange-fleshed sweetpotato cultivar in good quality for a longer period of time. The study involved two factors, viz storage bag type and storage period. The experiment tried to address (1) a sustainable seed system in sweetpotato and (2) production stimulated by cultural practises. Vines were stored in polyethylene bag without holes, polyethylene bag with 12 holes and dry hessian bag over 3, 6, 9, 12 and 15 days at an ambient temperature of 250C. The incremental cost-effectiveness ratio (ICER) was calculated to determine the cost-effectiveness of using the polyethylene bags with holes or without and the hessian bags. The results of the study showed that hessian bags store vines over a longer period (12 days) compared to the polyethylene bags without holes (6 days); however, hessian bags are more expensive. Polyethylene bags can still be used for transporting and storing vines if the farmer is within a short distance from the seed source and plants the vines within the 6 day period. Polyethylene bags with 12 holes are recommended since they allow maximum air circulation and extend storage period (9 days). Optimum planting techniques can help sweetpotato farmers to achieve optimal yields. Newly developed cultivars from the Agricultural Research Council have not been tested to determine the best planting techniques. A trial was conducted to investigate the effect of two planting orientations (horizontal and vertical) and different vine length (2, 3 and 4 subterranean nodes) of 3 released varieties, Blesbock, Bophelo and Ndou on storage root yield of sweetpotato. A factorial experiment was laid out in a randomised complete block design with three replications during two cropping seasons (2014/15 and 2015/16). The data collected in both experiments included total storage root yield, marketable storage root yield, and number of storage roots per size class. Significant differences were detected for the interaction of all factors on the number roots per size class, total storage root yield, and total average root weight. Vertical and horizontal planting per se showed no significance differences for all the sweetpotato root growth parameters such as total yield. However, the more the number of subterainian node the more yield we got for the commercial cultivar Blesbok. Paclobutrazol (PBZ) is a plant growth retardant hormone. This hormone might, therefore, be useful for sweetpotato growers to achieve higher plant populations per hectare because it would reduce the plant canopy. However, it should also be checked if the PBZ application does not affect nutrient content of roots and leaves, especially the beta-carotene content of the orange-fleshed cultivars. The objective of this study was, therefore, to determine the effect of PBZ on sweetpotato shoot growth, storage root yield and beta-carotene content of sweetpotato leaves and storage roots. The field trial was repeated over two seasons, namely 2014/15 and 2015/16 crop seasons. These field experiments were arranged as a split plot experiment. The main plots were allocated to four PBZ levels, laid-out as randomised complete block design, and treatment combinations of two cultivars (Bophelo and Blesbok) and two plants spacing (20 cm and 30 cm) were randomly allocated into the subplots. The PBZ levels applied were 0, 100, 200 and 300 mg of active ingredient per litre of water, sprayed 4 weeks after planting. Root yield and bioweight accumulation were recorded. In addition, a pot experiment was set up using cultivar Bophelo and 0 mg, 100 mg and 200 mg/l active ingredient of PBZ sprayed 4 weeks after planting. Leaf and root samples (150g and 400g of a composite sample respectively) were collected and analysed for total phenols, total flavonoids, vitamin C and beta-carotene. The field trial results revealed significant differences (P<0.05) in leaf and stem weight of the plants treated with different PBZ levels applied. The control (0 mg PBZ rate) had the highest leaf and stem weight. There were no significant differences in root yield among the PBZ levels. The results of leaf and root analysis for the Bophelo cultivar showed significant differences (P<0.05) in total phenols, total flavonoids, vitamin C and beta-carotene in leaf samples of the three levels of PBZ applied. The beta-carotene for the leaf increased at the 100 mg/l PBZ level. As the paclobutrazol level increased there was a reduction in the beta-carotene content for the roots from 18,91 to 13,97 to 10,77 mg/100g dry weight for 0 mg/l, 100 mg/l and 200mg/l, respectively. Application of PBZ to sweetpotato reduces the bioweight yield but does not have an effect on the root yield. Thus a higher plant density can be used to achieve higher yield and returns on investment. The leaves can be used as a relish and can be sold to supplement income. However, PBZ further reduces the beta-carotene content of the roots and is not recommended for use with orange-fleshed cultivars, where the objective is to enrich food with vitamin A. Sweetpotato weevils (Cylas puncticollis and C. formicarius) are notorious pests of sweetpotato. It causes severe losses in marketable root yield. The study tested the use of Beauveria bassiana (Balsamo Vuillemin) (Deuteromycota: Hyphomycetes) for controlling sweetpotato weevils. Experiments were conducted over two cropping seasons using four treatments: 1) dipping sweetpotato cuttings in solution of 1 g/litre B. bassiana before planting, 2) spraying with a registered chemical (Deltametrin) at 50 ml/100 litre of water starting 2 weeks after panting at biweekly basis for four months after planting, 3) spraying the leaves with B. bassiana (10 g/100 litre) starting 2 weeks on biweekly basis for four months after planting; and 4) control (sprayed with water). The research was arranged as split plot experiment, where the pest management treatments assigned to the main plots as per Latin square design and the three sweetpotato cultivars (Bophelo, Ndou and Blesbok) were randomly put in the subplot in each main plot. Spraying with B. bassiana significantly reduced the percentage of insect-damaged roots (9.95percent) compared to the control (22.48percent). Dipping sweetpotato cuttings in solution of 1 g/litre B. bassiana before planting did not significantly reduce the number of insect damaged roots (18.63percent insect damage). There was no significant difference between the chemical spray (8.2percent insect damage) and spraying with B. bassiana (9.95percent insect damage). The investigation indicated that B. bassiana can be considered as an alternative control method for the sweetpotato weevil. Use of biological control is less expensive and beneficial to reduce adverse effects on the environment and human health. , Thesis (PhD) -- Faculty of Science and Agriculture, 2019
- Full Text:
- Date Issued: 2019-09
Surveillance study on pathogenic Acinetobacter species in freshwater environment of the Amathole and Chris Hani District Municipalities, Eastern Cape, South Africa
- Authors: Adewoyin, Mary Ayobami
- Date: 2019-09
- Subjects: Acinetobacter infections , Acinetobacter , Nosocomial infections
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19836 , vital:43257
- Description: This study evaluates the occurrence of medically relevant Acinetobacter species in three rivers, namely; Keiskamma, Tyhume and Great Fish in the Eastern Cape Province, South Africa in one year sampling regime (April 2017 - March 2018). The physicochemical parameters (pH, temperature (TEM), electrical conductivity (EC), total dissolved solids (TDS), salinity (SAL), total suspended solids (TSS), turbidity (TBS), dissolved oxygen (DO) and biological oxygen demand (BOD)) of the water bodies were measured. The presumptive Acinetobacter species recovered from the freshwater resources were recorded and further confirmed using molecular techniques. Similarly, confirmed isolates were subjected to speciation using species-specific primer sets for A. baumannii and A. nosocomialis. Also, virulence genes namely; afa/draBC, epsA, fimH, OmpA, PAI, sfa/focDE, and traT in the two Acinetobacter species were also determined using molecular method. In addition, the antibiogram characteristics of A. baumannii and A. nosocomialis isolated from the water samples were determined using standard methods. The antibiotic susceptibility test was performed using a panel of 12 antibiotics belonging to the aminoglycosides (amikacin, AK and gentamicin, GM), β-lactam/β-lactamase-inhibitor combinations (piperacillin-tazobactam, PTZ), cephems (ceftazidime, CAZ, cefotaxime, CTX, and cefepime, CPM), carbapenems (imipenem, IMI and meropenem, MEM), fluoroquinolones (ciprofloxacin, CIP), folate pathway inhibitors (Trimethoprim/sulfamethoxazole, TS), lipopeptides (Polymyxin B, PB) and tetracyclines (tetracycline, TET). Similarly, antibiotic resistance genes (ARGs) present in the Acinetobacter isolates were investigated including aminoglycoside resistance genes (aacC2, aphA1 and aphA2), β-lactamases resistance genes (blaTEM, blaSHV, blaOXA-1-like, blaCTX-M(GROUP 1), blaCTX-M(GROUP 2), blaCTX-M(GROUP 9), blaVEB, blaGES, blaPER, blaCTX-M-8/-25, blaOXA-48-like, blaVIM, blaIMP and blaKPC), fluoroquinolones resistance genes (qnrA, qnrB, qnrC, qnrD and qnrS), sulfonamide resistance genes (sul1 and sul2), and tetracycline resistance genes (tetA, tetB, tetC, tetM, tetL and tetO). The pH, EC, TDS, SAL, TEMP, TSS, TBS, DO, and BOD for Tyhume River ranged as follows: 7.2-7.7, 125-141 μS/cm, 62-71mg/L, 0.06- 0.07 PSU, 11.3-20.2oC, 30.0-89.6 mg/L, 35.0-96.0 NTU, 8.2-9.8 mg/L, 2.0-4.2 mg/L, while for Great Fish River, the parameters were 8.0-8.2, 274-369 μS/cm, 137-184mg/L, 0.13-0.18PSU, 12.7-22.3oC, 44.3-99.4 mg/L, 48.0-214.0 NTU, 7.8-9.9 mg/L, 3.1- 4.9 mg/L, and at Keiskamma River they were 7.5-7.9, 153.2-285.0 μS/cm, 86-143 mg/L, 0.07-0.14 PSU, 11.0-21.4oC, 27.0- 55.6 mg/L, 31-61 NTU, 8.3-9.8 mg/L, 3.0-6.0 mg/L. A total of 1107 presumptive Acinetobacter spp. were recovered from the rivers sampled of which 428, 370 and 309 isolates were recovered from Tyhume, Great Fish and Keiskamma rivers respectively. However, only 844 was confirmed positive for the genus Acinetobacter and are recovered in the proportions 285 (77 percent), 219 (70.9 percent) and 340 (79 percent) from Great Fish, Keiskemma and Tyhume rivers respectively. Our finding revealed that 410 (48.58 percent) and 23 (2.7 percent) of the isolates were confirmed to be A. baumannii and A. nosocomalis respectively. Also, 308 (75.12 percent percent) A. baumannii and 3 (13.04 percent) A. nosocomialis isolates exhibited one or more virulence genes out of the seven tested, whereas 102 (24.88 percent) and 20 (86.95 percent) of the A. baumannii and A. nosocomialis isolates did not harbour any virulence gene. Additionally, OmpA was the most prevalent (p<0.05) virulence gene found in A. baumannii with 69 (45.10 percent), 52 (50.98 percent) and 77 (49.68 percent) isolates from Great Fish, Keiskamma and Tyhume rivers respectively. The rates of susceptibilities of A. baumannii and A. nosocomialis to the antibiotics followed the order; Piperacillin-tazobactam (72.8 percent ; 73.9 percent), Ceftazidime (70.5 percent ; 91.3 percent), Cefotaxime (16.8 percent ; 17.4 percent), Cefepime (88.5 percent ; 95.7 percent), Imipenem (95.9 percent ; 100 percent ), Meropenem (92.7 percent ; 91.3 percent), Amikacin (97.6 percent ; 91.3 percent), Gentamicin (89.8 percent ; 87 percent), Polymyxin B (84.4 percent ; 91.3 percent), Tetracycline (74.7 percent ; 78.3 percent), Ciprofloxacin (75.9 percent ; 78.3 percent) and Trimethoprim/sulfamethoxazole (74.0 percent ; 73.9 percent) respectively. Both A. baumannii and A. nosocomialis were highly susceptible to all the antimicrobials tested except cefotaxime where 64 percent and 78 percent intermediate responses were observed in the species. At least 10 isolates of A. baumannii were resistant against each of the antibiotics used. The modal multiple antibiotics resistance phenotypes (MARPs) for Acinetobacter spp. was MARP 3 (29.87 percent) and the least was MARP 10 and 11 (2.6 percent each). The antimicrobial resistance index (ARI) was higher at two sampling sites KE2 (0.33) and TY1 (0.22). Similarly, MARI showed that sampling sites KE2 was a hotspot for multidrug-resistant Acinetobacter spp. Of the five classes of ARGs studied, there was a widespread of β-lactamases (blaTEM) in the two Acinetobacter species, followed by sul2, which were detected in 67 (63.2 percent) and 44 (49.4 percent) isolates respectively, across the rivers studied. We conclude that aquatic resources of the study community are important reservoirs of pathogenic Acinetobacter species and antibiotic resistance determinants. The occurrence of clinically-important Acinetobacter species suggests possible contamination of these selected rivers which are consumed by humans and livestock, as well as being used for irrigation system, and this constitutes a risk to public health. It also shows that A. baumannii and A. nosocomialis can thrive in the aquatic environment. This study suggests that direct utilization of water from these sources for domestic and other purposes without any form of pre-treatment should be avoided. It is, therefore, necessary for regulatory authorities to monitor the release of domestic and industrial wastewater into these water bodies in order to prevent outbreaks of epidemics. , Thesis (PhD) (Microbiology) -- University of Fort Hare, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Adewoyin, Mary Ayobami
- Date: 2019-09
- Subjects: Acinetobacter infections , Acinetobacter , Nosocomial infections
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19836 , vital:43257
- Description: This study evaluates the occurrence of medically relevant Acinetobacter species in three rivers, namely; Keiskamma, Tyhume and Great Fish in the Eastern Cape Province, South Africa in one year sampling regime (April 2017 - March 2018). The physicochemical parameters (pH, temperature (TEM), electrical conductivity (EC), total dissolved solids (TDS), salinity (SAL), total suspended solids (TSS), turbidity (TBS), dissolved oxygen (DO) and biological oxygen demand (BOD)) of the water bodies were measured. The presumptive Acinetobacter species recovered from the freshwater resources were recorded and further confirmed using molecular techniques. Similarly, confirmed isolates were subjected to speciation using species-specific primer sets for A. baumannii and A. nosocomialis. Also, virulence genes namely; afa/draBC, epsA, fimH, OmpA, PAI, sfa/focDE, and traT in the two Acinetobacter species were also determined using molecular method. In addition, the antibiogram characteristics of A. baumannii and A. nosocomialis isolated from the water samples were determined using standard methods. The antibiotic susceptibility test was performed using a panel of 12 antibiotics belonging to the aminoglycosides (amikacin, AK and gentamicin, GM), β-lactam/β-lactamase-inhibitor combinations (piperacillin-tazobactam, PTZ), cephems (ceftazidime, CAZ, cefotaxime, CTX, and cefepime, CPM), carbapenems (imipenem, IMI and meropenem, MEM), fluoroquinolones (ciprofloxacin, CIP), folate pathway inhibitors (Trimethoprim/sulfamethoxazole, TS), lipopeptides (Polymyxin B, PB) and tetracyclines (tetracycline, TET). Similarly, antibiotic resistance genes (ARGs) present in the Acinetobacter isolates were investigated including aminoglycoside resistance genes (aacC2, aphA1 and aphA2), β-lactamases resistance genes (blaTEM, blaSHV, blaOXA-1-like, blaCTX-M(GROUP 1), blaCTX-M(GROUP 2), blaCTX-M(GROUP 9), blaVEB, blaGES, blaPER, blaCTX-M-8/-25, blaOXA-48-like, blaVIM, blaIMP and blaKPC), fluoroquinolones resistance genes (qnrA, qnrB, qnrC, qnrD and qnrS), sulfonamide resistance genes (sul1 and sul2), and tetracycline resistance genes (tetA, tetB, tetC, tetM, tetL and tetO). The pH, EC, TDS, SAL, TEMP, TSS, TBS, DO, and BOD for Tyhume River ranged as follows: 7.2-7.7, 125-141 μS/cm, 62-71mg/L, 0.06- 0.07 PSU, 11.3-20.2oC, 30.0-89.6 mg/L, 35.0-96.0 NTU, 8.2-9.8 mg/L, 2.0-4.2 mg/L, while for Great Fish River, the parameters were 8.0-8.2, 274-369 μS/cm, 137-184mg/L, 0.13-0.18PSU, 12.7-22.3oC, 44.3-99.4 mg/L, 48.0-214.0 NTU, 7.8-9.9 mg/L, 3.1- 4.9 mg/L, and at Keiskamma River they were 7.5-7.9, 153.2-285.0 μS/cm, 86-143 mg/L, 0.07-0.14 PSU, 11.0-21.4oC, 27.0- 55.6 mg/L, 31-61 NTU, 8.3-9.8 mg/L, 3.0-6.0 mg/L. A total of 1107 presumptive Acinetobacter spp. were recovered from the rivers sampled of which 428, 370 and 309 isolates were recovered from Tyhume, Great Fish and Keiskamma rivers respectively. However, only 844 was confirmed positive for the genus Acinetobacter and are recovered in the proportions 285 (77 percent), 219 (70.9 percent) and 340 (79 percent) from Great Fish, Keiskemma and Tyhume rivers respectively. Our finding revealed that 410 (48.58 percent) and 23 (2.7 percent) of the isolates were confirmed to be A. baumannii and A. nosocomalis respectively. Also, 308 (75.12 percent percent) A. baumannii and 3 (13.04 percent) A. nosocomialis isolates exhibited one or more virulence genes out of the seven tested, whereas 102 (24.88 percent) and 20 (86.95 percent) of the A. baumannii and A. nosocomialis isolates did not harbour any virulence gene. Additionally, OmpA was the most prevalent (p<0.05) virulence gene found in A. baumannii with 69 (45.10 percent), 52 (50.98 percent) and 77 (49.68 percent) isolates from Great Fish, Keiskamma and Tyhume rivers respectively. The rates of susceptibilities of A. baumannii and A. nosocomialis to the antibiotics followed the order; Piperacillin-tazobactam (72.8 percent ; 73.9 percent), Ceftazidime (70.5 percent ; 91.3 percent), Cefotaxime (16.8 percent ; 17.4 percent), Cefepime (88.5 percent ; 95.7 percent), Imipenem (95.9 percent ; 100 percent ), Meropenem (92.7 percent ; 91.3 percent), Amikacin (97.6 percent ; 91.3 percent), Gentamicin (89.8 percent ; 87 percent), Polymyxin B (84.4 percent ; 91.3 percent), Tetracycline (74.7 percent ; 78.3 percent), Ciprofloxacin (75.9 percent ; 78.3 percent) and Trimethoprim/sulfamethoxazole (74.0 percent ; 73.9 percent) respectively. Both A. baumannii and A. nosocomialis were highly susceptible to all the antimicrobials tested except cefotaxime where 64 percent and 78 percent intermediate responses were observed in the species. At least 10 isolates of A. baumannii were resistant against each of the antibiotics used. The modal multiple antibiotics resistance phenotypes (MARPs) for Acinetobacter spp. was MARP 3 (29.87 percent) and the least was MARP 10 and 11 (2.6 percent each). The antimicrobial resistance index (ARI) was higher at two sampling sites KE2 (0.33) and TY1 (0.22). Similarly, MARI showed that sampling sites KE2 was a hotspot for multidrug-resistant Acinetobacter spp. Of the five classes of ARGs studied, there was a widespread of β-lactamases (blaTEM) in the two Acinetobacter species, followed by sul2, which were detected in 67 (63.2 percent) and 44 (49.4 percent) isolates respectively, across the rivers studied. We conclude that aquatic resources of the study community are important reservoirs of pathogenic Acinetobacter species and antibiotic resistance determinants. The occurrence of clinically-important Acinetobacter species suggests possible contamination of these selected rivers which are consumed by humans and livestock, as well as being used for irrigation system, and this constitutes a risk to public health. It also shows that A. baumannii and A. nosocomialis can thrive in the aquatic environment. This study suggests that direct utilization of water from these sources for domestic and other purposes without any form of pre-treatment should be avoided. It is, therefore, necessary for regulatory authorities to monitor the release of domestic and industrial wastewater into these water bodies in order to prevent outbreaks of epidemics. , Thesis (PhD) (Microbiology) -- University of Fort Hare, 2019
- Full Text:
- Date Issued: 2019-09
The Effect of training and development on employee performance in the Buffalo City Municipality in the Eastern Cape of South Africa
- Authors: Adom, Richard Kwame
- Date: 2019-09
- Subjects: Employees -- Training of , Performance -- Management , Employees -- Rating of
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20153 , vital:45377
- Description: Training and development are regarded as significant tools for any organisation to achieve the target goals and objectives. These goals and objectives are heavily and highly dependent on its workforce. In the literature, it is well documented that many organisations invest in employees’ training and development to enhance their performance and the growth of the organisation. Many organisations, however, including the Buffalo City Municipality, regard training and staff development as needless expenditure, and always do little, or make no effort to improve employees’ technical know-how. Such actions incapacitate staff to adapt to the ever-changing working environment and rapid technological innovation. This study employed purposive sampling technique to draw a sample of 70 employees from a total of 150, through structured interviews and a self-administered questionnaire, to examine the effect of training and development on employees’ performance in the Buffalo City Municipality, situated in the Eastern Cape of South Africa. The findings of the study generally revealed that training and development does not only increase employees’ performance, but assists in developing the overall personality of employees by making them more productive through building their senses of teamwork, boosting the attitude of staff which is the most critical component in attaining profit, and developing a cordial relationship and connectivity between management and the workforce. The study, therefore, recommended that the municipality, as a matter of urgency, earmarks a certain percentage of its resources for employees training and development, based on identified skills gaps to sharpen employees’ skills, competencies, capabilities and technical know-how, to capacitate them to cope with the ever-changing working environment and innovations, and to enhance their motivation, satisfaction and performance. , Thesis (MPA) -- Faculty of Management and Commerce, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Adom, Richard Kwame
- Date: 2019-09
- Subjects: Employees -- Training of , Performance -- Management , Employees -- Rating of
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20153 , vital:45377
- Description: Training and development are regarded as significant tools for any organisation to achieve the target goals and objectives. These goals and objectives are heavily and highly dependent on its workforce. In the literature, it is well documented that many organisations invest in employees’ training and development to enhance their performance and the growth of the organisation. Many organisations, however, including the Buffalo City Municipality, regard training and staff development as needless expenditure, and always do little, or make no effort to improve employees’ technical know-how. Such actions incapacitate staff to adapt to the ever-changing working environment and rapid technological innovation. This study employed purposive sampling technique to draw a sample of 70 employees from a total of 150, through structured interviews and a self-administered questionnaire, to examine the effect of training and development on employees’ performance in the Buffalo City Municipality, situated in the Eastern Cape of South Africa. The findings of the study generally revealed that training and development does not only increase employees’ performance, but assists in developing the overall personality of employees by making them more productive through building their senses of teamwork, boosting the attitude of staff which is the most critical component in attaining profit, and developing a cordial relationship and connectivity between management and the workforce. The study, therefore, recommended that the municipality, as a matter of urgency, earmarks a certain percentage of its resources for employees training and development, based on identified skills gaps to sharpen employees’ skills, competencies, capabilities and technical know-how, to capacitate them to cope with the ever-changing working environment and innovations, and to enhance their motivation, satisfaction and performance. , Thesis (MPA) -- Faculty of Management and Commerce, 2019
- Full Text:
- Date Issued: 2019-09
Treatment adherence among tuberculosis patients in Nelson Mandela District, Eastern Cape Province
- Authors: Sohuma, Ntombifikile
- Date: 2019-09
- Subjects: Patient compliance , Tuberculosis -- Patients , Tuberculosis -- Mortality
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/22957 , vital:53254
- Description: Tuberculosis (TB) morbidity and mortality rates remain high globally and are even higher in Africa. The key factor that continues to drive the burden of disease is poor compliance / adherence to treatment regimens. This study aimed to identify determinants that continue to drive poor treatment adherence among low-income communities in South Africa. Methods A cross-sectional survey was conducted, drawing on TB patients who were defaulting on treatment. An assessment tool with several socio-economic and disease-related determinants was administered to participants. Data was entered into MS Excel and analysed in SPSS version 24. Results Regarding health services factors generally, all participants agreed that health service delivery was conducted in a conducive environment and that facilities were reasonably close to their places of residence. Participants demonstrated that health workers do not provide adequate information regarding medications that patients receive. Of patients cited, 11percent indicated that they stopped medications owing to health services. When questioned, participants reported that health workers shouted at them for coming late (e.g. after lunch) and that waiting times were very long. Approximately 85percent of the study participants were more likely to adhere poorly to TB medication owing to various factors such as comorbidities and personal perceptions of wellbeing / health status. Comorbidities are high among patients, with 58percent taking other medication while on TB treatment. Self-prognosis on wellbeing is significantly high, and 44percent reported a tendency to stop medication when they were seemingly in recovery. The unemployment rate among respondents was 78percent, with limited education: 69percent had below matric. Of the respondents, 40percent showed an unsatisfactory perception of their social and economic life, 55percent were somewhat satisfied and only 5percent were very satisfied. These indicators constitute a highly vulnerable community that depends heavily on subsidised healthcare from the state. Adherence to TB treatment is mainly influenced by poverty, the health service provider–patient relationship and perceived stigma. Limited family support was noted towards the treatment of patients. , Thesis (MPH) -- Faculty of Health Sciences, 2019
- Full Text:
- Date Issued: 2019-09
- Authors: Sohuma, Ntombifikile
- Date: 2019-09
- Subjects: Patient compliance , Tuberculosis -- Patients , Tuberculosis -- Mortality
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/22957 , vital:53254
- Description: Tuberculosis (TB) morbidity and mortality rates remain high globally and are even higher in Africa. The key factor that continues to drive the burden of disease is poor compliance / adherence to treatment regimens. This study aimed to identify determinants that continue to drive poor treatment adherence among low-income communities in South Africa. Methods A cross-sectional survey was conducted, drawing on TB patients who were defaulting on treatment. An assessment tool with several socio-economic and disease-related determinants was administered to participants. Data was entered into MS Excel and analysed in SPSS version 24. Results Regarding health services factors generally, all participants agreed that health service delivery was conducted in a conducive environment and that facilities were reasonably close to their places of residence. Participants demonstrated that health workers do not provide adequate information regarding medications that patients receive. Of patients cited, 11percent indicated that they stopped medications owing to health services. When questioned, participants reported that health workers shouted at them for coming late (e.g. after lunch) and that waiting times were very long. Approximately 85percent of the study participants were more likely to adhere poorly to TB medication owing to various factors such as comorbidities and personal perceptions of wellbeing / health status. Comorbidities are high among patients, with 58percent taking other medication while on TB treatment. Self-prognosis on wellbeing is significantly high, and 44percent reported a tendency to stop medication when they were seemingly in recovery. The unemployment rate among respondents was 78percent, with limited education: 69percent had below matric. Of the respondents, 40percent showed an unsatisfactory perception of their social and economic life, 55percent were somewhat satisfied and only 5percent were very satisfied. These indicators constitute a highly vulnerable community that depends heavily on subsidised healthcare from the state. Adherence to TB treatment is mainly influenced by poverty, the health service provider–patient relationship and perceived stigma. Limited family support was noted towards the treatment of patients. , Thesis (MPH) -- Faculty of Health Sciences, 2019
- Full Text:
- Date Issued: 2019-09
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