Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa
- Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
Conference eQoboqobo
- Lubabalo, Ntshoko and Dr Mitchell Cox, Student Researcher
- Authors: Lubabalo, Ntshoko and Dr Mitchell Cox , Student Researcher
- Date: 27-02-2014
- Subjects: Oral history Africa , Healers South Africa Interviews
- Language: Xhosa , English
- Type: Oral histories , Sound recordings , Interviews , audio recording
- Identifier: http://hdl.handle.net/10962/458416 , vital:75741 , KHA-008
- Description: uLubabalo Ntshoko nongumphandi uthetha ngokubaluleka komhlaba kunye nemdlela ekutyetywe ngayo eQoboqobo. UDr Michell Cox waseRhodes obhale incwadi ethi Voices for the Forest incwadi le ibhalwe ngemithi yonke esiyibilisayo nokubaluleka kwendalo empilweni yethu , Lubabalo Ntshoko and Dr Mitchell Cox speaks about the importance of nature and natural resources in Qoboqobo at a conference and she references her book titled Voices Of the Forest
- Full Text: false
- Date Issued: 27-02-2014
- Authors: Lubabalo, Ntshoko and Dr Mitchell Cox , Student Researcher
- Date: 27-02-2014
- Subjects: Oral history Africa , Healers South Africa Interviews
- Language: Xhosa , English
- Type: Oral histories , Sound recordings , Interviews , audio recording
- Identifier: http://hdl.handle.net/10962/458416 , vital:75741 , KHA-008
- Description: uLubabalo Ntshoko nongumphandi uthetha ngokubaluleka komhlaba kunye nemdlela ekutyetywe ngayo eQoboqobo. UDr Michell Cox waseRhodes obhale incwadi ethi Voices for the Forest incwadi le ibhalwe ngemithi yonke esiyibilisayo nokubaluleka kwendalo empilweni yethu , Lubabalo Ntshoko and Dr Mitchell Cox speaks about the importance of nature and natural resources in Qoboqobo at a conference and she references her book titled Voices Of the Forest
- Full Text: false
- Date Issued: 27-02-2014
VIVA Comrade Moss
- NUMSA
- Authors: NUMSA
- Date: 25 April
- Subjects: NUMSA
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/109640 , vital:33163 , SALHA 14b : NUMSA
- Description: The last 'Trial Update" reported on Comrade Moss’s case until mid-March. This report covers the period from that time up until 25th April.STADLER - "ANC EXPERT" Brigadier Stadler testified that the ANC had made certain calls to organisations within the country which, according to his "research", had been followed by organisations in the country. As examples he gave the ANC’s call for ungovernability, the formation of organs of people’s power and the destruction of town councils. During cross-examination Stadler conceded that he was not able to give examples of any direct link between the ANC and organisations inside the coutry but he said an inference could be drawn by looking at the ANC’s call and the behaviour of organisations inside the country.
- Full Text:
- Date Issued: 25 April
- Authors: NUMSA
- Date: 25 April
- Subjects: NUMSA
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/109640 , vital:33163 , SALHA 14b : NUMSA
- Description: The last 'Trial Update" reported on Comrade Moss’s case until mid-March. This report covers the period from that time up until 25th April.STADLER - "ANC EXPERT" Brigadier Stadler testified that the ANC had made certain calls to organisations within the country which, according to his "research", had been followed by organisations in the country. As examples he gave the ANC’s call for ungovernability, the formation of organs of people’s power and the destruction of town councils. During cross-examination Stadler conceded that he was not able to give examples of any direct link between the ANC and organisations inside the coutry but he said an inference could be drawn by looking at the ANC’s call and the behaviour of organisations inside the country.
- Full Text:
- Date Issued: 25 April
Morphology, histology and histochemistry of the digestive tract of the Banded tilapia, Tilapia sparrmanii (Perciformes: Cichlidae)
- Grace E. Okuthe, Bongile Bhomela
- Authors: Grace E. Okuthe , Bongile Bhomela
- Date: December 23, 2020
- Subjects: Fish, gastrointestinal tract, histo-architecture
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2425 , vital:41881
- Description: ABSTRACT. This study described anatomical, histological and histochemical features of the mucosal layer of the digestive tract of Tilapia sparrmanii Smith, 1840, an omnivorous freshwater fish endemic to Southern Africa. This species exhibited a short thick oesophagus with long deep longitudinal folds (466.68 ± 16.91 µm), and a thick (173.50 ± 10.92 µm) muscular layer that allow the passage of large food items. The mucosa was lined with stratified secretory epithelium rich in goblet cells that secreted neutral and acid mucins. The stomach was a sac-like structure with simple tubular glands surrounded by connective tissue. The mucosa was lined with simple columnar epithelium and the lamina propria exhibited a well-developed layer of gastric glands that occupied the entire length of the cardio-fundic region. The stomach mucosa consisted of epithelial cells with intense neutral mucin secretion which protects against gastric juice. Neck cells of gastric glands synthesized neutral and acid mucins. The intestine was highly coiled and presented a complex pattern of transversal folds internally (villi). Villi length decreased progressively from the anterior to the posterior intestine (p < 0.0001). Tunica muscularis of the mid-intestine had the thinnest thickness among all parts of the intestine (p < 0.0001). Goblet cells whose numbers increased towards the rectum secreted both acid and neutral mucins. The results indicate structural similarities of T. sparrmanii GIT with other tilapia species and will be useful for understanding the physiology of the digestive systems as well as functional components of the GIT.
- Full Text:
- Authors: Grace E. Okuthe , Bongile Bhomela
- Date: December 23, 2020
- Subjects: Fish, gastrointestinal tract, histo-architecture
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2425 , vital:41881
- Description: ABSTRACT. This study described anatomical, histological and histochemical features of the mucosal layer of the digestive tract of Tilapia sparrmanii Smith, 1840, an omnivorous freshwater fish endemic to Southern Africa. This species exhibited a short thick oesophagus with long deep longitudinal folds (466.68 ± 16.91 µm), and a thick (173.50 ± 10.92 µm) muscular layer that allow the passage of large food items. The mucosa was lined with stratified secretory epithelium rich in goblet cells that secreted neutral and acid mucins. The stomach was a sac-like structure with simple tubular glands surrounded by connective tissue. The mucosa was lined with simple columnar epithelium and the lamina propria exhibited a well-developed layer of gastric glands that occupied the entire length of the cardio-fundic region. The stomach mucosa consisted of epithelial cells with intense neutral mucin secretion which protects against gastric juice. Neck cells of gastric glands synthesized neutral and acid mucins. The intestine was highly coiled and presented a complex pattern of transversal folds internally (villi). Villi length decreased progressively from the anterior to the posterior intestine (p < 0.0001). Tunica muscularis of the mid-intestine had the thinnest thickness among all parts of the intestine (p < 0.0001). Goblet cells whose numbers increased towards the rectum secreted both acid and neutral mucins. The results indicate structural similarities of T. sparrmanii GIT with other tilapia species and will be useful for understanding the physiology of the digestive systems as well as functional components of the GIT.
- Full Text:
Erythrina caffra - kaffirboom
- Authors: Skead, C J (Cuthbert John)
- Date: 23-08-1959
- Subjects: Erythrina caffra - South Africa -- Photographs , Trees -- South Africa -- Photographs
- Language: English
- Type: mixed material , photographs , landscape photographs
- Identifier: http://hdl.handle.net/10962/119690 , vital:34772
- Description: Caption "In Woodlands forest between King Williams Town and Stutterheim. 23-09-1959.”
- Full Text: false
- Date Issued: 23-08-1959
- Authors: Skead, C J (Cuthbert John)
- Date: 23-08-1959
- Subjects: Erythrina caffra - South Africa -- Photographs , Trees -- South Africa -- Photographs
- Language: English
- Type: mixed material , photographs , landscape photographs
- Identifier: http://hdl.handle.net/10962/119690 , vital:34772
- Description: Caption "In Woodlands forest between King Williams Town and Stutterheim. 23-09-1959.”
- Full Text: false
- Date Issued: 23-08-1959
The prevalence of insufficient iodine intake in pregnancy in Africa: Protocol for a systematic review and meta-analysis
- Charles Bitamazire Businge, Benjamin Longo-Mbenza, Andre Pascal Kengne
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
- Date Issued: 22-8-2019
- Authors: Charles Bitamazire Businge , Benjamin Longo-Mbenza , Andre Pascal Kengne
- Date: 22-8-2019
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/3387 , vital:43338 , https://systematicreviewsjournal.biomedcentral.com/articles/10.1186/s13643-019-1092-7#citeas
- Description: Background: Insufficient iodine intake in pregnancy is associated with many adverse pregnancy outcomes. About 90% of African countries are at risk of iodine deficiency due to poor soils and dietary goitrogens. Pregnancy predisposes to insufficient iodine nutrition secondary to increased physiological demand and increased renal loss. Iodine deficiency is re-emerging in countries thought to be replete with pregnant women being the most affected. This review seeks to identify the degree of iodine nutrition in pregnancy on the entire African continent before and after the implementation of national iodization programmes. Methods: A systematic search of published literature will be conducted for observational studies that directly determined the prevalence of insufficient iodine intake among pregnant women in Africa. Electronic databases and grey literature will be searched for baseline data before the implementation of population-based iodine supplementation and for follow-up data up to December 2018. Screening of identified articles and data extraction will be conducted independently by two investigators. Risk of bias and methodological quality of the included studies will be assessed using a risk of bias tool. Appropriate meta-analytic techniques will be used to pool prevalence estimates from studies with similar features, overall and by major characteristics including the region of the study, time period (before and after implementation of iodization programmes), sample size and age. Heterogeneity of the estimates across studies will be quantified and publication bias investigated. This protocol is reported according to Preferred Reporting Items for Systematic reviews and Meta-Analysis protocols (PRISMA-P) 2015 guidelines. Discussion This review will help ascertain the impact of national iodization programmes on the iodine nutrition status in pregnancy in Africa and advise policy on the necessity for monitoring and mitigating iodine deficiency in pregnancy in Africa. This review is part of a thesis that will be submitted to the Faculty of Health Sciences, University of Cape Town, for the award of a PhD in Medicine whose protocol has been granted ethics approval (UCT HREC 135/2018). In addition, the results will be published in a peer-reviewed journal.
- Full Text:
- Date Issued: 22-8-2019
The heterogeneous effects of macroeconomic and financial factors on financial deepening in Africa: evidence from a method of moments quantile regression analysis
- Sanga, Bahati, Aziakpono, Meshach J
- Authors: Sanga, Bahati , Aziakpono, Meshach J
- Date: 2025
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/469930 , vital:77308 , https://www.emerald.com/insight/content/doi/10.1108/jfep-07-2023-0199/full/html
- Description: This paper aims to investigate the heterogeneous effects of macroeconomic and financial factors across various distributions of financial deepening in 22 African countries over the past two decades (2000–2019). The paper uses a recent method of moments quantile regression, which accounts for the often overlooked heterogeneity effects. The analysis focuses on the banking sector, which is predominant in Africa, using a broad range of macroeconomic and financial indicators.
- Full Text:
- Date Issued: 2025
- Authors: Sanga, Bahati , Aziakpono, Meshach J
- Date: 2025
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/469930 , vital:77308 , https://www.emerald.com/insight/content/doi/10.1108/jfep-07-2023-0199/full/html
- Description: This paper aims to investigate the heterogeneous effects of macroeconomic and financial factors across various distributions of financial deepening in 22 African countries over the past two decades (2000–2019). The paper uses a recent method of moments quantile regression, which accounts for the often overlooked heterogeneity effects. The analysis focuses on the banking sector, which is predominant in Africa, using a broad range of macroeconomic and financial indicators.
- Full Text:
- Date Issued: 2025
Honorary Citation for the Conferment of Doctor of Laws honoris causa - Tandabantu Tennyson August
- Rhodes University, Martin, Tom
- Authors: Rhodes University , Martin, Tom
- Date: 2024-14-05
- Subjects: Uncatalogued
- Language: English
- Type: Speeches , Transcripts, speech , text
- Identifier: http://hdl.handle.net/10962/432591 , vital:72885
- Description: Rhodes University Honorary Doctorate Citation (5 April 2024) Tandabantu Tennyson August: 1980s. South Africa. In the Bantustans of the time, like the Transkei, the youth have little reason for hope. They have much reason for despair. Their choices seem limited to the politics of struggle or to crime, an attempt to gain some kind of advantage from the meagre and unacceptable options on offer. In those bleak times, what was needed was visionaries. People who could see beyond the current circumstances to a different future and imagine how best to prepare for it. Not as idealists, but as pragmatists. What would happen once circumstances change, once the struggle had succeeded? Would we be ready for it? What qualities would the youth need to have in order to both benefit from and participate in a new dispensation? One such visionary was Tandabantu Tennyson August, and it is my distinct pleasure to have the opportunity to tell you more about him. Born in 1948, Mr. August matriculated from Healdtown High School, before pursuing higher education at the University of Transkei, where obtained a Bachelor of Arts degree majoring in English and Geography. This was followed by Comparative Curriculum Studies at the University of South Africa, alongside obtaining teaching certificates from Lovedale and Cicira teacher training colleges, with majors in Physical Science, Mathematics, Science, and Mathematics. This solid educational foundation laid the groundwork for his impactful career in education. He taught at Wongalethu High, St Johns College and was deputy principal at Norwood, before taking up his first principalship, at Thanga High School in 1980. From 1984 to 1990 he was principal of Ndamase High School. During his tenure there, the matric pass rate never fell below 90%. From 1991 to 1995 he was principal Isivivane High School during which time the school was promoted from a primary to a high school. Following his distinguished service at schools, he worked at the Eastern Cape Department of Basic Education, first as a deputy chief education specialist (1996 – 2006) and then as chief education specialist (2006-2010). During that time, he played a pivotal role in establishing (in conjunction with Rhodes University) and being the provincial coordinator the Advanced Certificate in Education in English, followed by ACEs in mathematical literacy and life orientation. In 2016 he received a provincial teaching award for excellence. In 2017 he was awarded the Kade Asmal award for excellence in teaching. To this day, he remains an Executive member of the eastern cape Education advisory council. Quite a career. But there’s something I haven’t mentioned. He was also an accomplished rugby player of international standing. While at Lovedale College, he was selected to represent Border and then the South African African Rugby Board, playing England in Gqeberha in 1972, in a team that would later be known as the black springboks. He eventually received his green and gold springbok blazer as just recognition of his achievement. He incorporated his love of sport into his vision for the youth under his care, as part of his overriding mission of educating the whole person. He got everyone involved in sport, if not as a player, then as a supporter. Especially during his time as principal of Ndamase High School, I am led to believe, it was extremely intimidating for opposing teams to play them. Not only at Ndamase home games, but especially at their away games. There would be one bus for the team players, and six buses for the supporters. From 1986 – 1990: he was president of the Transkei schools` sports union (TSSU). But there’s yet another thing I haven’t mentioned. In 1981, just as his career as an educational leader was really taking off, he was involved in a terrible motor vehicle accident. It left him permanently disabled and, for much of his life, a wheelchair user. Rather than stopping him, it simply added another string to his bow, now as an advocate for the disabled. From 2012 – 2022: Chairperson Eastern Cape South African National Association For Special Education (SANASE): this organization cater for the education and wellbeing of learners with disabilities and special needs. In 2023, he was the national deputy president of the same organisation. From 2013 – to date: he is a Board Member on the National Council of & for persons with disabilities (NCPD). I had the pleasure of an extensive conversation with one of Mr August’s former pupils at Ndamase High, Professor Deyi Mbelani, the current director of Rhodes University’s Institute for the Study of Englishes in Africa. He told me many stories about Mr August’s leadership, that I only wish I had time to share with you now. I put it to Professor Mbelani that, having read about Mr August, the word that stood out for me was “dedication.” He agreed that “dedication” was the word, but that I must not leave out another: “vision”. It is Mr August’s status as a visionary that best defines him, he said. Going back over the above, I think he is right. Mr Chancellor I have the honour to request you to confer the degree of Doctor of Laws honoris causa on the Mr Tandabantu Tennyson August. *The above citation draws heavily on documents submitted as part of the honorary doctorate nomination process and others compiled by the Division of Communications and Advancement. - Tom Martin.
- Full Text:
- Date Issued: 2024-14-05
- Authors: Rhodes University , Martin, Tom
- Date: 2024-14-05
- Subjects: Uncatalogued
- Language: English
- Type: Speeches , Transcripts, speech , text
- Identifier: http://hdl.handle.net/10962/432591 , vital:72885
- Description: Rhodes University Honorary Doctorate Citation (5 April 2024) Tandabantu Tennyson August: 1980s. South Africa. In the Bantustans of the time, like the Transkei, the youth have little reason for hope. They have much reason for despair. Their choices seem limited to the politics of struggle or to crime, an attempt to gain some kind of advantage from the meagre and unacceptable options on offer. In those bleak times, what was needed was visionaries. People who could see beyond the current circumstances to a different future and imagine how best to prepare for it. Not as idealists, but as pragmatists. What would happen once circumstances change, once the struggle had succeeded? Would we be ready for it? What qualities would the youth need to have in order to both benefit from and participate in a new dispensation? One such visionary was Tandabantu Tennyson August, and it is my distinct pleasure to have the opportunity to tell you more about him. Born in 1948, Mr. August matriculated from Healdtown High School, before pursuing higher education at the University of Transkei, where obtained a Bachelor of Arts degree majoring in English and Geography. This was followed by Comparative Curriculum Studies at the University of South Africa, alongside obtaining teaching certificates from Lovedale and Cicira teacher training colleges, with majors in Physical Science, Mathematics, Science, and Mathematics. This solid educational foundation laid the groundwork for his impactful career in education. He taught at Wongalethu High, St Johns College and was deputy principal at Norwood, before taking up his first principalship, at Thanga High School in 1980. From 1984 to 1990 he was principal of Ndamase High School. During his tenure there, the matric pass rate never fell below 90%. From 1991 to 1995 he was principal Isivivane High School during which time the school was promoted from a primary to a high school. Following his distinguished service at schools, he worked at the Eastern Cape Department of Basic Education, first as a deputy chief education specialist (1996 – 2006) and then as chief education specialist (2006-2010). During that time, he played a pivotal role in establishing (in conjunction with Rhodes University) and being the provincial coordinator the Advanced Certificate in Education in English, followed by ACEs in mathematical literacy and life orientation. In 2016 he received a provincial teaching award for excellence. In 2017 he was awarded the Kade Asmal award for excellence in teaching. To this day, he remains an Executive member of the eastern cape Education advisory council. Quite a career. But there’s something I haven’t mentioned. He was also an accomplished rugby player of international standing. While at Lovedale College, he was selected to represent Border and then the South African African Rugby Board, playing England in Gqeberha in 1972, in a team that would later be known as the black springboks. He eventually received his green and gold springbok blazer as just recognition of his achievement. He incorporated his love of sport into his vision for the youth under his care, as part of his overriding mission of educating the whole person. He got everyone involved in sport, if not as a player, then as a supporter. Especially during his time as principal of Ndamase High School, I am led to believe, it was extremely intimidating for opposing teams to play them. Not only at Ndamase home games, but especially at their away games. There would be one bus for the team players, and six buses for the supporters. From 1986 – 1990: he was president of the Transkei schools` sports union (TSSU). But there’s yet another thing I haven’t mentioned. In 1981, just as his career as an educational leader was really taking off, he was involved in a terrible motor vehicle accident. It left him permanently disabled and, for much of his life, a wheelchair user. Rather than stopping him, it simply added another string to his bow, now as an advocate for the disabled. From 2012 – 2022: Chairperson Eastern Cape South African National Association For Special Education (SANASE): this organization cater for the education and wellbeing of learners with disabilities and special needs. In 2023, he was the national deputy president of the same organisation. From 2013 – to date: he is a Board Member on the National Council of & for persons with disabilities (NCPD). I had the pleasure of an extensive conversation with one of Mr August’s former pupils at Ndamase High, Professor Deyi Mbelani, the current director of Rhodes University’s Institute for the Study of Englishes in Africa. He told me many stories about Mr August’s leadership, that I only wish I had time to share with you now. I put it to Professor Mbelani that, having read about Mr August, the word that stood out for me was “dedication.” He agreed that “dedication” was the word, but that I must not leave out another: “vision”. It is Mr August’s status as a visionary that best defines him, he said. Going back over the above, I think he is right. Mr Chancellor I have the honour to request you to confer the degree of Doctor of Laws honoris causa on the Mr Tandabantu Tennyson August. *The above citation draws heavily on documents submitted as part of the honorary doctorate nomination process and others compiled by the Division of Communications and Advancement. - Tom Martin.
- Full Text:
- Date Issued: 2024-14-05
A framework for designing a gamified system that promotes knowledge of water sustainability
- Authors: Kunjuzwa, Dumani Tau
- Date: 2024-12
- Subjects: Computer science , User interfaces (Computer systems) , Water-supply -- Management
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69349 , vital:77216
- Description: Water shortages are becoming increasingly common and raising awareness thereof has been proposed as a key strategy for empowering citizens with knowledge relevant to promoting water sustainability. This knowledge should incorporate indigenous knowledge, which is unique and confined to a particular culture. Indigenous knowledge has been recognised as the key to sustainable social and economic development. Knowledge incorporated within appropriate technologies can be a transformative solution to promote sustainable consumption of natural resources. This study aimed to develop a framework with strong theoretical connections that can be used as a guide for designing and developing technological solutions for promoting knowledge, attitude, and practices (KAP) of water sustainability. Gamification, serious games, and community social networks were identified as appropriate technologies for implementing these solutions. Design Science Research (DSR) was an appropriate research methodology for this research. As the requirement of the DSR methodology, a framework was designed that considered relevant theories. The two theories (Self-Determination Theory and Social Capital Theory) were incorporated into the framework. The framework can guide other researchers in developing gamified systems that promote the KAPs of water sustainability. This framework was the theoretical artefact of the study. The research context was the Eastern Cape Province of South Africa where the data was collected from two municipalities namely, the Nelson Mandela Bay and Buffalo City Metro, which have both experienced water shortages in recent years. Quantitative and qualitative data collection methods were used. Qualitative data was collected from brainstorming sessions and expert reviews that were conducted with various stakeholders that assisted with designing and evaluating the prototype designs of the gamified solution. For the design of the study’s artefacts, a participatory research approach was adopted. Participants were recruited to complete an online survey, which ran for three months. The findings revealed that citizens’ knowledge is closely correlated to the attitudes and practices of citizens regarding water sustainability. In addition, the findings showed that the knowledge of the citizens in the Eastern Cape Province was relatively low on water practices such as conservation and filtering methods. These results were considered in the design of the practical artefact and the instantiation findings. One limitation of this study was the limited number of indigenous participants in the survey. Other limitations were the lack of experts with indigenous knowledge and the lack of indigenous knowledge documentation. The promotion of water sustainability knowledge in communities would be more effective with the use of gamification and social community networks. In principle, the enhancement in water sustainability knowledge would promote the citizens’ good practices and attitudes (behavioural) related to water sustainability. Since 2015, a significant portion of South Africa's Eastern Cape Province has been suffering from a severe drought. This province is one of the regions of the country where weather patterns have changed dramatically, and as a result, most households were left vulnerable to the water shortage problem. While the broad topic of water sustainability is widely discussed in the literature, the role of appropriate technologies in promoting knowledge of water sustainability is still under-researched. This gap provided a significant potential for investigation by sustainability to design a technological and theoretical solution for promoting knowledge of water sustainability. This study contributes to the body of knowledge by delivering three DSR artefacts namely, a Framework for Designing a Gamified System that Promotes Knowledge of Water Sustainability; the design guidelines that supplement the framework, and a practical instantiation of the framework. , Thesis (DPhil) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Kunjuzwa, Dumani Tau
- Date: 2024-12
- Subjects: Computer science , User interfaces (Computer systems) , Water-supply -- Management
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69349 , vital:77216
- Description: Water shortages are becoming increasingly common and raising awareness thereof has been proposed as a key strategy for empowering citizens with knowledge relevant to promoting water sustainability. This knowledge should incorporate indigenous knowledge, which is unique and confined to a particular culture. Indigenous knowledge has been recognised as the key to sustainable social and economic development. Knowledge incorporated within appropriate technologies can be a transformative solution to promote sustainable consumption of natural resources. This study aimed to develop a framework with strong theoretical connections that can be used as a guide for designing and developing technological solutions for promoting knowledge, attitude, and practices (KAP) of water sustainability. Gamification, serious games, and community social networks were identified as appropriate technologies for implementing these solutions. Design Science Research (DSR) was an appropriate research methodology for this research. As the requirement of the DSR methodology, a framework was designed that considered relevant theories. The two theories (Self-Determination Theory and Social Capital Theory) were incorporated into the framework. The framework can guide other researchers in developing gamified systems that promote the KAPs of water sustainability. This framework was the theoretical artefact of the study. The research context was the Eastern Cape Province of South Africa where the data was collected from two municipalities namely, the Nelson Mandela Bay and Buffalo City Metro, which have both experienced water shortages in recent years. Quantitative and qualitative data collection methods were used. Qualitative data was collected from brainstorming sessions and expert reviews that were conducted with various stakeholders that assisted with designing and evaluating the prototype designs of the gamified solution. For the design of the study’s artefacts, a participatory research approach was adopted. Participants were recruited to complete an online survey, which ran for three months. The findings revealed that citizens’ knowledge is closely correlated to the attitudes and practices of citizens regarding water sustainability. In addition, the findings showed that the knowledge of the citizens in the Eastern Cape Province was relatively low on water practices such as conservation and filtering methods. These results were considered in the design of the practical artefact and the instantiation findings. One limitation of this study was the limited number of indigenous participants in the survey. Other limitations were the lack of experts with indigenous knowledge and the lack of indigenous knowledge documentation. The promotion of water sustainability knowledge in communities would be more effective with the use of gamification and social community networks. In principle, the enhancement in water sustainability knowledge would promote the citizens’ good practices and attitudes (behavioural) related to water sustainability. Since 2015, a significant portion of South Africa's Eastern Cape Province has been suffering from a severe drought. This province is one of the regions of the country where weather patterns have changed dramatically, and as a result, most households were left vulnerable to the water shortage problem. While the broad topic of water sustainability is widely discussed in the literature, the role of appropriate technologies in promoting knowledge of water sustainability is still under-researched. This gap provided a significant potential for investigation by sustainability to design a technological and theoretical solution for promoting knowledge of water sustainability. This study contributes to the body of knowledge by delivering three DSR artefacts namely, a Framework for Designing a Gamified System that Promotes Knowledge of Water Sustainability; the design guidelines that supplement the framework, and a practical instantiation of the framework. , Thesis (DPhil) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 2024
- Full Text:
- Date Issued: 2024-12
A multistep synthesis of zidovudine in continuous flow systems
- Authors: Kwakhanya, Mkwakwi
- Date: 2024-12
- Subjects: HIV -- South Africa , HIV infections -- Diagnosis , Antiretroviral agents -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69393 , vital:77251
- Description: Universal access to antiretroviral drugs is still a challenge in developing nations. This promoted an investigation into an efficient process towards the synthesis of zidovudine (AZT), a nucleoside reverse transcriptase inhibitor (NRTI) that is used to treat HIV/AIDS. It was the first antiretroviral drug approved by the FDA and it is still widely used today. AZT works by blocking the enzyme reverse transcriptase, which is used by HIV to copy its genetic material. This prevents HIV from replicating and spreading throughout the body. AZT is usually taken in combination with other antiretroviral drugs. AZT was originally used as a single-drug therapy, but it is no longer recommended for this purpose as HIV can become resistant to AZT if it is used alone. Consequently, AZT is most commonly used as part of a combination therapy; this means that it is taken with other antiretroviral drugs. This helps to prevent HIV from becoming resistant to AZT. AZT can be used to prevent mother-to-child transmission of HIV; it is usually given to HIV-positive women during pregnancy and childbirth. AZT can also be used to treat post-exposure prophylaxis (PEP); this is a course of antiretroviral drugs that is taken after a possible exposure to HIV. The application of continuous flow microreactors to reaction chemistries, such as this, could provide a potentially practical solution that could be used to meet the rising needs for antiretroviral drugs in Africa and to give new opportunities for efficient local drug manufacture. Consequently, the focus of this research was to develop safe and efficient processes towards AZT by taking advantage of micro reactor technology. Furthermore, since there is no literature on continuous flow synthesis of this drug, this research extensively unearthed interesting continuous flow processes towards zidovudine. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Kwakhanya, Mkwakwi
- Date: 2024-12
- Subjects: HIV -- South Africa , HIV infections -- Diagnosis , Antiretroviral agents -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69393 , vital:77251
- Description: Universal access to antiretroviral drugs is still a challenge in developing nations. This promoted an investigation into an efficient process towards the synthesis of zidovudine (AZT), a nucleoside reverse transcriptase inhibitor (NRTI) that is used to treat HIV/AIDS. It was the first antiretroviral drug approved by the FDA and it is still widely used today. AZT works by blocking the enzyme reverse transcriptase, which is used by HIV to copy its genetic material. This prevents HIV from replicating and spreading throughout the body. AZT is usually taken in combination with other antiretroviral drugs. AZT was originally used as a single-drug therapy, but it is no longer recommended for this purpose as HIV can become resistant to AZT if it is used alone. Consequently, AZT is most commonly used as part of a combination therapy; this means that it is taken with other antiretroviral drugs. This helps to prevent HIV from becoming resistant to AZT. AZT can be used to prevent mother-to-child transmission of HIV; it is usually given to HIV-positive women during pregnancy and childbirth. AZT can also be used to treat post-exposure prophylaxis (PEP); this is a course of antiretroviral drugs that is taken after a possible exposure to HIV. The application of continuous flow microreactors to reaction chemistries, such as this, could provide a potentially practical solution that could be used to meet the rising needs for antiretroviral drugs in Africa and to give new opportunities for efficient local drug manufacture. Consequently, the focus of this research was to develop safe and efficient processes towards AZT by taking advantage of micro reactor technology. Furthermore, since there is no literature on continuous flow synthesis of this drug, this research extensively unearthed interesting continuous flow processes towards zidovudine. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
A strategy towards an it governance-focused implementation of Egovernment initiatives in the Eastern Cape provincial government
- Authors: Magade, Siyabonga
- Date: 2024-12
- Subjects: Internet in public administration , Government communication systems , Public administration -- Data processing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68725 , vital:77085
- Description: ICT plays a very important role in transforming organisations as well as governments around the world through eGovernment. A good implementation of eGovernment promises to transform and enhance the provision of services to citizens. The benefits of eGovernment will enable the transparency of government and engender the trust of its citizens therein. Citizens will have access to online government services that will minimise the long queues that citizens need to endure to access government services. eGovernment implementation requires ICT technologies, however, dependency on the implementation of ICT technologies to drive eGovernment initiatives will not alone succeed in transforming the delivery of services in government. Therefore, the implementation of ICT technologies in government must be focused on good IT governance principles. Such principles enable the executive council and management to direct and control the application both now and in the future of use of ICT in government. Therefore, this study develops a strategy artefact that is focused on good IT governance principles to assist in the execution of eGovernment programs in the Eastern Cape Provincial Government. , Thesis (MPhil) -- Faculty of Engineering, the Built Environment, and Technology, School of Information Technology, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Magade, Siyabonga
- Date: 2024-12
- Subjects: Internet in public administration , Government communication systems , Public administration -- Data processing
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68725 , vital:77085
- Description: ICT plays a very important role in transforming organisations as well as governments around the world through eGovernment. A good implementation of eGovernment promises to transform and enhance the provision of services to citizens. The benefits of eGovernment will enable the transparency of government and engender the trust of its citizens therein. Citizens will have access to online government services that will minimise the long queues that citizens need to endure to access government services. eGovernment implementation requires ICT technologies, however, dependency on the implementation of ICT technologies to drive eGovernment initiatives will not alone succeed in transforming the delivery of services in government. Therefore, the implementation of ICT technologies in government must be focused on good IT governance principles. Such principles enable the executive council and management to direct and control the application both now and in the future of use of ICT in government. Therefore, this study develops a strategy artefact that is focused on good IT governance principles to assist in the execution of eGovernment programs in the Eastern Cape Provincial Government. , Thesis (MPhil) -- Faculty of Engineering, the Built Environment, and Technology, School of Information Technology, 2024
- Full Text:
- Date Issued: 2024-12
Achievement in mathematics through self-directed learning facilitation: an impact evaluation of an offline technological approach
- Authors: Zinn, Paméla Olive
- Date: 2024-12
- Subjects: Mathematics -- Study and teaching (Secondary) -- South Africa , Mathematical ability , Academic achievement -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68385 , vital:76992
- Description: International assessments indicate that mathematics education in South Africa is still in a crisis of significant proportions. This is reflected in the low pass rate of Grade 12 mathematics learners in South Africa in general, and in the Eastern Cape Province in particular. In response, the Govan Mbeki Mathematics Development Center (GMMDC) of the Nelson Mandela University (NMU) in the Eastern Cape developed an intervention strategy. A Techno-Blended support Model for teaching and learning mathematics (TBM), used as an intervention strategy, provides learners with potential from disadvantaged communities and under-resourced schools with 21st-century education technology. The defining features of the strategy which distinguishes it from others are that it is technology-enhanced, providing the learner access to mathematics teaching and learning without the need for Internet connectivity, that is, offline, incorporating blended learning into the approach. This ex-post study, using secondary analysis, evaluates the impact of the defining features of the strategy on learners' self-directed learning skills acquisition or enhancement for mathematics learning. Firstly, statistical analysis of secondary qualitative and quantitative data in a mixed methods approach, with primary qualitative data integrated into the secondary qualitative data employing quantification, is undertaken. The quantified data is then compared with the quantitative data (methodologic triangulation technique). Secondly, the theory-based Contribution Analysis is undertaken to increase the validity and credibility of the findings of the statistical analysis, providing an additional layer of triangulation. The study revealed that providing 21st-century digital education technology for use offline, within a blended learning environment, leads to a significant improvement in learner mathematics knowledge; conceptual understanding; positive self-perceptions; and finally, to the required intrinsic motivation to learn. Analysed data, obtained from the original study, indicates a positive correlation between learners who participated in the intervention strategy and enrolment in STEM-related courses at tertiary institutes. , Thesis (DPhil) -- Faculty of Education, School of Postgraduate education, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Zinn, Paméla Olive
- Date: 2024-12
- Subjects: Mathematics -- Study and teaching (Secondary) -- South Africa , Mathematical ability , Academic achievement -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68385 , vital:76992
- Description: International assessments indicate that mathematics education in South Africa is still in a crisis of significant proportions. This is reflected in the low pass rate of Grade 12 mathematics learners in South Africa in general, and in the Eastern Cape Province in particular. In response, the Govan Mbeki Mathematics Development Center (GMMDC) of the Nelson Mandela University (NMU) in the Eastern Cape developed an intervention strategy. A Techno-Blended support Model for teaching and learning mathematics (TBM), used as an intervention strategy, provides learners with potential from disadvantaged communities and under-resourced schools with 21st-century education technology. The defining features of the strategy which distinguishes it from others are that it is technology-enhanced, providing the learner access to mathematics teaching and learning without the need for Internet connectivity, that is, offline, incorporating blended learning into the approach. This ex-post study, using secondary analysis, evaluates the impact of the defining features of the strategy on learners' self-directed learning skills acquisition or enhancement for mathematics learning. Firstly, statistical analysis of secondary qualitative and quantitative data in a mixed methods approach, with primary qualitative data integrated into the secondary qualitative data employing quantification, is undertaken. The quantified data is then compared with the quantitative data (methodologic triangulation technique). Secondly, the theory-based Contribution Analysis is undertaken to increase the validity and credibility of the findings of the statistical analysis, providing an additional layer of triangulation. The study revealed that providing 21st-century digital education technology for use offline, within a blended learning environment, leads to a significant improvement in learner mathematics knowledge; conceptual understanding; positive self-perceptions; and finally, to the required intrinsic motivation to learn. Analysed data, obtained from the original study, indicates a positive correlation between learners who participated in the intervention strategy and enrolment in STEM-related courses at tertiary institutes. , Thesis (DPhil) -- Faculty of Education, School of Postgraduate education, 2024
- Full Text:
- Date Issued: 2024-12
Ammendments to the labour law relations act 66 of 1995 to addressing violent and intractable strikes
- Authors: Gavu, Siphelele
- Date: 2024-12
- Subjects: Labor laws and legislation -- South Africa , Strikes and lockouts -- Law and legislation -- South Africa , Labor disputes -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68692 , vital:77082
- Description: The main research question for this treatise is to determine whether the South African legal framework provides effective regulations in curbing violent and intractable strikes. To answer the research question, the research explored the evolution of the right to strike in South Africa, analysing its socio-economic impact and the transition from a repressive regime to a democratic one. The study assessed international, regional, and national legal frameworks, focusing on the right to strike and its regulation. Amendments to the LRA, including introducing rules for picketing, mandatory secret ballots for strikes, and advisory arbitration for dysfunctional strikes, were evaluated. The findings indicate that while these amendments represent a significant step in addressing the issues of violent strikes in an employment context, their effectiveness is limited. Persistent challenges include the frequent disregard by trade unions and their members for legal measures. This reflects the deeper socio- economic and political issues. The study suggests that the effectiveness of these legislative changes is hindered by ongoing social and economic inequalities, highlighting the need for broader socio-political solutions beyond legislative amendments. This comprehensive analysis explains the complexities surrounding strike actions in South Africa and the balance between upholding labour rights and maintaining workplace discipline and order. , Thesis (LLM) -- Faculty of Law, School of Mercantile Law, 2024
- Full Text:
- Date Issued: 2024-12
Ammendments to the labour law relations act 66 of 1995 to addressing violent and intractable strikes
- Authors: Gavu, Siphelele
- Date: 2024-12
- Subjects: Labor laws and legislation -- South Africa , Strikes and lockouts -- Law and legislation -- South Africa , Labor disputes -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68692 , vital:77082
- Description: The main research question for this treatise is to determine whether the South African legal framework provides effective regulations in curbing violent and intractable strikes. To answer the research question, the research explored the evolution of the right to strike in South Africa, analysing its socio-economic impact and the transition from a repressive regime to a democratic one. The study assessed international, regional, and national legal frameworks, focusing on the right to strike and its regulation. Amendments to the LRA, including introducing rules for picketing, mandatory secret ballots for strikes, and advisory arbitration for dysfunctional strikes, were evaluated. The findings indicate that while these amendments represent a significant step in addressing the issues of violent strikes in an employment context, their effectiveness is limited. Persistent challenges include the frequent disregard by trade unions and their members for legal measures. This reflects the deeper socio- economic and political issues. The study suggests that the effectiveness of these legislative changes is hindered by ongoing social and economic inequalities, highlighting the need for broader socio-political solutions beyond legislative amendments. This comprehensive analysis explains the complexities surrounding strike actions in South Africa and the balance between upholding labour rights and maintaining workplace discipline and order. , Thesis (LLM) -- Faculty of Law, School of Mercantile Law, 2024
- Full Text:
- Date Issued: 2024-12
Associations between patterns of wild ungulate patch use, soil carbon and albedo in montane grasslands
- Authors: Mc Gregor, Steven
- Date: 2024-12
- Subjects: Grassland ecology , Global environmental change , Climatic changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69382 , vital:77250
- Description: Grassy ecosystems are essential for human survival, providing key services including food production, water provisioning and moderating climate. Yet, grassy ecosystems remain undervalued due to misconceptions that they are remnants of degraded states of forest – a view that continues to influence climate change policies. This thesis explores the links between wild, free-roaming ungulate grazers and climate drivers, including carbon storage and surface albedo (i.e., reflectance of solar radiation), through their impacts on vegetation and soils. Using black wildebeest (Connochaetes gnou) as a model wild species, I compared their grazing patterns in an Afromontane grassland in the eastern Karoo, South Africa, to those of short-duration cattle grazing systems in similar nearby grasslands (Chapter 2). While short-duration grazing aims to mimic spatiotemporal wild ungulate grazing patterns to supposedly enhance ecosystem functioning and soil carbon stocks, I found that wildebeest had ~50% shorter grazing durations and much shorter rest intervals (1-5 days versus 60-365 days), revealing key differences in grazing patterns that may affect vegetation and climate feedbacks. Next, I examined spatial variations in soil organic carbon (SOC) stocks between grass growth forms that differ in grazing tolerance (Chapter 3). Red grass (Themeda triandra) tussock patches, sensitive to frequent grazing, had higher SOC to a soil depth of 20 cm (61.45 ± 1.59 Mg C·ha-1)than intensively grazed, prostrate-growing Cynodon dactylon grazing lawns (55.43 ± 3.40Mg C·ha-1), likely due to greater shading and soil moisture beneath tussocks which drives microbial decomposition. Seasonal albedo variations were then assessed across distinct grassland patch types among seasons to determine whether albedo varies seasonally at fine patch-scales between grass patches, between shrub and grass patches, and with grazing (Chapter 4). Albedo was lower during the growing season compared to dormancy, and was consistently lower in dwarf shrub (Pentzia incana) encroached patches compared to grass patches. No albedo differences between grazed and less-grazed tussock grass patches of the same species were found, although intensively grazed grazing lawns had consistently higher albedo than most patch types. Finally, I evaluated trade-offs between plant carbon, albedo, and their impacts on radiative forcing (i.e., atmospheric warming/cooling) resulting from patch type changes commonly found in grassy ecosystems (Chapter 5). The loss of perennial grass cover resulted in the highest net positive (warming) effect, mostly due to reduced root biomass. Additionally, shrub encroachment into all patches lowered albedo, but led to negative (cooling) effects from shrub encroachment into bare ground patches due to biomass gains. This thesis challenges current views of grassy landscapes and short-duration grazing systems, emphasizing the need to rethink climate change mitigation strategies to prioritize maintaining heterogeneity, while enhancing carbon sequestration and albedo in grassy ecosystems. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Mc Gregor, Steven
- Date: 2024-12
- Subjects: Grassland ecology , Global environmental change , Climatic changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/69382 , vital:77250
- Description: Grassy ecosystems are essential for human survival, providing key services including food production, water provisioning and moderating climate. Yet, grassy ecosystems remain undervalued due to misconceptions that they are remnants of degraded states of forest – a view that continues to influence climate change policies. This thesis explores the links between wild, free-roaming ungulate grazers and climate drivers, including carbon storage and surface albedo (i.e., reflectance of solar radiation), through their impacts on vegetation and soils. Using black wildebeest (Connochaetes gnou) as a model wild species, I compared their grazing patterns in an Afromontane grassland in the eastern Karoo, South Africa, to those of short-duration cattle grazing systems in similar nearby grasslands (Chapter 2). While short-duration grazing aims to mimic spatiotemporal wild ungulate grazing patterns to supposedly enhance ecosystem functioning and soil carbon stocks, I found that wildebeest had ~50% shorter grazing durations and much shorter rest intervals (1-5 days versus 60-365 days), revealing key differences in grazing patterns that may affect vegetation and climate feedbacks. Next, I examined spatial variations in soil organic carbon (SOC) stocks between grass growth forms that differ in grazing tolerance (Chapter 3). Red grass (Themeda triandra) tussock patches, sensitive to frequent grazing, had higher SOC to a soil depth of 20 cm (61.45 ± 1.59 Mg C·ha-1)than intensively grazed, prostrate-growing Cynodon dactylon grazing lawns (55.43 ± 3.40Mg C·ha-1), likely due to greater shading and soil moisture beneath tussocks which drives microbial decomposition. Seasonal albedo variations were then assessed across distinct grassland patch types among seasons to determine whether albedo varies seasonally at fine patch-scales between grass patches, between shrub and grass patches, and with grazing (Chapter 4). Albedo was lower during the growing season compared to dormancy, and was consistently lower in dwarf shrub (Pentzia incana) encroached patches compared to grass patches. No albedo differences between grazed and less-grazed tussock grass patches of the same species were found, although intensively grazed grazing lawns had consistently higher albedo than most patch types. Finally, I evaluated trade-offs between plant carbon, albedo, and their impacts on radiative forcing (i.e., atmospheric warming/cooling) resulting from patch type changes commonly found in grassy ecosystems (Chapter 5). The loss of perennial grass cover resulted in the highest net positive (warming) effect, mostly due to reduced root biomass. Additionally, shrub encroachment into all patches lowered albedo, but led to negative (cooling) effects from shrub encroachment into bare ground patches due to biomass gains. This thesis challenges current views of grassy landscapes and short-duration grazing systems, emphasizing the need to rethink climate change mitigation strategies to prioritize maintaining heterogeneity, while enhancing carbon sequestration and albedo in grassy ecosystems. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2024
- Full Text:
- Date Issued: 2024-12
Causative factors for poor contractor performance in schools infrastructure projects
- Authors: Baba, Vuyiseka
- Date: 2024-12
- Subjects: Project management , Project management -- Evaluation , School facilities -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68703 , vital:77083
- Description: The public sector infrastructure departments in South Africa rely on construction companies as the major stakeholders in the implementation and maintenance of infrastructure facilities including schools. The Limpopo Province is faced with a serious challenge of underperforming contractors. This in turn affects the Provincial Government’s effective delivery of the much-needed quality infrastructure and facilities. A significant number of schools in the province are faced with infrastructure challenges including sanitation facilities, deteriorating school buildings, storm damage, inadequate infrastructure for special schools, water provision, and general maintenance. This study sought to understand the challenges faced by contractors and further explored whether the effective application of project management methodologies can have a positive impact on improving the current underperformance. Due to the nature of the study, a qualitative research method was employed to collect data. An interpretivist paradigm was adopted as the research philosophy, as it allowed for a flexible and in-depth investigation approach. The study utilized an exploratory research design. The targeted population for the study was carefully selected to obtain relevant and reliable data, and a purposive sampling technique was chosen. Semi-structured interviews were conducted as the primary data collection method, enabling the collection of first-hand information. To ensure accuracy and prevent loss of information, the research process involved recording and transcribing the interviews, using an inductive analysis approach. Eighteen out of the twenty targeted participants were successfully interviewed, resulting in a response rate of 90%. The findings of the study revealed that poor planning, inadequate financial management, inexperienced staff, interference from stakeholders, and a misunderstanding of project scope were among the many factors contributing to poor contractor performance. Furthermore, contractors, particularly Small and Medium Enterprises (SMEs), faced serious challenges during project implementation, including a lack of proper training, budget constraints, and delayed payments from clients. Based on the study's findings, it is recommended that contractors invest in planning and cost management software, including project management methodologies such as Agile, Waterfall, and MS Project. Further, contractors are encouraged to employ adequately skilled and qualified site personnel. Clients should also consider supporting underperforming contractors by pairing them with proven-performing ones to encourage mentorship and other forms of capacity building. , Thesis (MSc) -- Faculty of Engineering, the Built Environment, and Technology, School of Building and Human Settlements Management, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Baba, Vuyiseka
- Date: 2024-12
- Subjects: Project management , Project management -- Evaluation , School facilities -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68703 , vital:77083
- Description: The public sector infrastructure departments in South Africa rely on construction companies as the major stakeholders in the implementation and maintenance of infrastructure facilities including schools. The Limpopo Province is faced with a serious challenge of underperforming contractors. This in turn affects the Provincial Government’s effective delivery of the much-needed quality infrastructure and facilities. A significant number of schools in the province are faced with infrastructure challenges including sanitation facilities, deteriorating school buildings, storm damage, inadequate infrastructure for special schools, water provision, and general maintenance. This study sought to understand the challenges faced by contractors and further explored whether the effective application of project management methodologies can have a positive impact on improving the current underperformance. Due to the nature of the study, a qualitative research method was employed to collect data. An interpretivist paradigm was adopted as the research philosophy, as it allowed for a flexible and in-depth investigation approach. The study utilized an exploratory research design. The targeted population for the study was carefully selected to obtain relevant and reliable data, and a purposive sampling technique was chosen. Semi-structured interviews were conducted as the primary data collection method, enabling the collection of first-hand information. To ensure accuracy and prevent loss of information, the research process involved recording and transcribing the interviews, using an inductive analysis approach. Eighteen out of the twenty targeted participants were successfully interviewed, resulting in a response rate of 90%. The findings of the study revealed that poor planning, inadequate financial management, inexperienced staff, interference from stakeholders, and a misunderstanding of project scope were among the many factors contributing to poor contractor performance. Furthermore, contractors, particularly Small and Medium Enterprises (SMEs), faced serious challenges during project implementation, including a lack of proper training, budget constraints, and delayed payments from clients. Based on the study's findings, it is recommended that contractors invest in planning and cost management software, including project management methodologies such as Agile, Waterfall, and MS Project. Further, contractors are encouraged to employ adequately skilled and qualified site personnel. Clients should also consider supporting underperforming contractors by pairing them with proven-performing ones to encourage mentorship and other forms of capacity building. , Thesis (MSc) -- Faculty of Engineering, the Built Environment, and Technology, School of Building and Human Settlements Management, 2024
- Full Text:
- Date Issued: 2024-12
Characterization of the molecular mechanism(s) of cannabinoid-induced paraptosis in breast cancer cells
- Authors: de la Harpe, Amy
- Date: 2024-12
- Subjects: Cannabinoids , Cannabinoids -- Therapeutic use , Cancer cells
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68818 , vital:77120
- Description: Natural products have been extensively studied for their anticancer potential, and several widely used anticancer drugs have natural origins. Many studies have demonstrated that natural compounds can induce paraptosis in various tumour cell lines. Paraptosis is a caspase-independent cell death mechanism characterised by cytoplasmic vacuolation arising from the endoplasmic reticulum (ER) and the mitochondria. The molecular mechanism of paraptosis is unclear; however, the literature indicates that dysregulation of calcium signalling plays an important role in paraptosis induction, particularly calcium-mediated signalling between the ER and mitochondria. This study aimed to identify and characterise the mechanism of cell death induced by a phytocannabinoid ratio which induced significant cytoplasmic vacuolation in the MCF7 breast cancer cell line. Several techniques were employed to address the aim of the study. The crystal violet assay was used to detect changes in viability. Several pathway inhibitors, as well as fluorescent staining and analysis using high-content screening (HCS), were used to measure the induction of various cell death mechanisms. Morphological changes were investigated using light and transmission electron microscopy. The phytocannabinoid ratio induced significant cell death and cytoplasmic vacuolation in MCF7 cells; however, the same trend was not observed in the MCF10A non-tumourigenic breast cell line. No cell cycle arrest, apoptosis, necrosis, autophagy, or ferroptosis induction was detected in MCF7 cells, suggesting that an alternative mechanism of cell death was induced. Vacuolation and cell death induced by the phytocannabinoid ratio were inhibited by cycloheximide, suggesting a dependence on protein synthesis, which is characteristic of paraptosis induction. The mechanism of paraptosis induction by the phytocannabinoid ratio was investigated, and it was found that treatment 1) induced ER dilation and mitochondrial swelling; 2) induced significant ER stress, mitochondrial calcium overload, and mitochondrial dysfunction which appeared to be mediated by the voltage-dependent anion channel (VDAC); and 3) significantly impaired all mitochondrial metabolic pathways. Overall, the data demonstrated that paraptosis induced by the cannabinoid ratio is mediated by calcium flux from the ER to the mitochondria. This highlighted a novel mechanism of phytocannabinoid-induced cell death and emphasised the anti-cancer potential of the use of phytocannabinoid ratios as opposed to individual phytocannabinoids, expanding their potential for use as anticancer agents. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: de la Harpe, Amy
- Date: 2024-12
- Subjects: Cannabinoids , Cannabinoids -- Therapeutic use , Cancer cells
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68818 , vital:77120
- Description: Natural products have been extensively studied for their anticancer potential, and several widely used anticancer drugs have natural origins. Many studies have demonstrated that natural compounds can induce paraptosis in various tumour cell lines. Paraptosis is a caspase-independent cell death mechanism characterised by cytoplasmic vacuolation arising from the endoplasmic reticulum (ER) and the mitochondria. The molecular mechanism of paraptosis is unclear; however, the literature indicates that dysregulation of calcium signalling plays an important role in paraptosis induction, particularly calcium-mediated signalling between the ER and mitochondria. This study aimed to identify and characterise the mechanism of cell death induced by a phytocannabinoid ratio which induced significant cytoplasmic vacuolation in the MCF7 breast cancer cell line. Several techniques were employed to address the aim of the study. The crystal violet assay was used to detect changes in viability. Several pathway inhibitors, as well as fluorescent staining and analysis using high-content screening (HCS), were used to measure the induction of various cell death mechanisms. Morphological changes were investigated using light and transmission electron microscopy. The phytocannabinoid ratio induced significant cell death and cytoplasmic vacuolation in MCF7 cells; however, the same trend was not observed in the MCF10A non-tumourigenic breast cell line. No cell cycle arrest, apoptosis, necrosis, autophagy, or ferroptosis induction was detected in MCF7 cells, suggesting that an alternative mechanism of cell death was induced. Vacuolation and cell death induced by the phytocannabinoid ratio were inhibited by cycloheximide, suggesting a dependence on protein synthesis, which is characteristic of paraptosis induction. The mechanism of paraptosis induction by the phytocannabinoid ratio was investigated, and it was found that treatment 1) induced ER dilation and mitochondrial swelling; 2) induced significant ER stress, mitochondrial calcium overload, and mitochondrial dysfunction which appeared to be mediated by the voltage-dependent anion channel (VDAC); and 3) significantly impaired all mitochondrial metabolic pathways. Overall, the data demonstrated that paraptosis induced by the cannabinoid ratio is mediated by calcium flux from the ER to the mitochondria. This highlighted a novel mechanism of phytocannabinoid-induced cell death and emphasised the anti-cancer potential of the use of phytocannabinoid ratios as opposed to individual phytocannabinoids, expanding their potential for use as anticancer agents. , Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
Co-generated learnings from hopes-an NRF grant funded university engagement project
- Authors: Quvile, Xola Bulumko
- Date: 2024-12
- Subjects: National Research Foundation (South Africa) , Research grants -- South Africa , Education, Higher -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68366 , vital:76990
- Description: The study had sought to understand the systemic and individual factors that facilitated or inhibited the NRF grant-funded HOPES Project. HOPES collaborated with the Centre for the Community School in the Faculty of Education, and various communities of practice (COPs) in existing school communities in the Eastern Cape Province of South Africa. The project applied a critical participatory action research work to develop a framework for school improvement and in the process contribute to making schools beacons of hope. This research provides insights into how an NRF community engagement grant project team (HOPES) navigated external and internal factors such as COVID-19, rules regulating the grant, and multiple realities of the stakeholders at play. The study was qualitative and used the critical participatory action research design. The study purposively and conveniently sampled seven HOPES Project team members to collect data via focus groups and individual interviews and collected secondary data from NRF HOPES annual project reports. The study findings improved the participants' understanding of their journey, and the co-generated knowledge shed light on how NFR and community engagement grant funders could promote enabling conditions towards achieving individual scholars and collective project outcomes. The study findings provide learnings for the Faculty of Education and the Nelson Mandela University on how extrinsic and intrinsic enablers and barriers can be addressed to optimise a project of this nature. , Thesis (MEd) -- Faculty of Education, School of Postgraduate Education, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Quvile, Xola Bulumko
- Date: 2024-12
- Subjects: National Research Foundation (South Africa) , Research grants -- South Africa , Education, Higher -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68366 , vital:76990
- Description: The study had sought to understand the systemic and individual factors that facilitated or inhibited the NRF grant-funded HOPES Project. HOPES collaborated with the Centre for the Community School in the Faculty of Education, and various communities of practice (COPs) in existing school communities in the Eastern Cape Province of South Africa. The project applied a critical participatory action research work to develop a framework for school improvement and in the process contribute to making schools beacons of hope. This research provides insights into how an NRF community engagement grant project team (HOPES) navigated external and internal factors such as COVID-19, rules regulating the grant, and multiple realities of the stakeholders at play. The study was qualitative and used the critical participatory action research design. The study purposively and conveniently sampled seven HOPES Project team members to collect data via focus groups and individual interviews and collected secondary data from NRF HOPES annual project reports. The study findings improved the participants' understanding of their journey, and the co-generated knowledge shed light on how NFR and community engagement grant funders could promote enabling conditions towards achieving individual scholars and collective project outcomes. The study findings provide learnings for the Faculty of Education and the Nelson Mandela University on how extrinsic and intrinsic enablers and barriers can be addressed to optimise a project of this nature. , Thesis (MEd) -- Faculty of Education, School of Postgraduate Education, 2024
- Full Text:
- Date Issued: 2024-12
Collaborative conservation governance in multifunctional landscapes: a contextual analysis of two South African biosphere reserves in the Boland
- Authors: Klaver, Michael
- Date: 2024-12
- Subjects: Unesco -- Programme on Man and the Biosphere , Nature conservation -- South Africa , Biosphere
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68924 , vital:77164
- Description: The Man and the Biosphere Programme (MAB) has been earmarked as one of the most promising ways of tackling the grand challenges of our time and achieving the Sustainable Development Goals. Furthermore, it may have the potential to make positive contributions to one of the most troubling conservation challenges of our time – winning spaces for large carnivores in human-dominated landscapes. This study aims to assess the interpretation and implementation of MAB in two South African Biosphere Reserves (BRs) and explore how BRs as landscape level collaborative initiatives can support Cape leopard conservation in the Boland region of the Western Cape, South Africa. This study is informed by two objectives. First, to understand how the chosen governance strategy and structure of two South African BRs have helped to achieve a context appropriate interpretation and actioning of MAB in the landscape. Second, to understand how landscape-level collaborative conservation initiatives support leopard conservation across a multifunctional landscape. This study takes on a mixed methods approach in which qualitative data takes priority and quantitative data is used for support in an embedded multiple-case study design. The sampling techniques used include purposive sampling of strategically targeted participants, i.e. those governing BRs and those leading the collaborative initiative and snowballing thereon. Data collection included a combination of semi-structure interviews (n=20) (the principal collection method), a review of archival, policy and other documents (n=15), and supported by a mixed methods survey questionnaire (n=5). Data were analysed through thematic and content analysis. Findings suggest that organisations working at a landscape-level utilise forms of adaptive co-management and/or governance as a means to being both adaptive and collaborative. Important themes which emerge include cross-sectoral approaches, networks, leadership and trust for building partnerships, shared visions and ‘filling gaps’ left by, or collaborating with the state to fulfil mutually beneficial objectives. This research provides empirical studies on MAB, important for sharing knowledge across the World Network of BRs, meeting the need for further research on collaborative initiatives in social-ecological systems and conservation programmes for large free-roaming carnivores in multifunctional landscapes. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Science & Management, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Klaver, Michael
- Date: 2024-12
- Subjects: Unesco -- Programme on Man and the Biosphere , Nature conservation -- South Africa , Biosphere
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68924 , vital:77164
- Description: The Man and the Biosphere Programme (MAB) has been earmarked as one of the most promising ways of tackling the grand challenges of our time and achieving the Sustainable Development Goals. Furthermore, it may have the potential to make positive contributions to one of the most troubling conservation challenges of our time – winning spaces for large carnivores in human-dominated landscapes. This study aims to assess the interpretation and implementation of MAB in two South African Biosphere Reserves (BRs) and explore how BRs as landscape level collaborative initiatives can support Cape leopard conservation in the Boland region of the Western Cape, South Africa. This study is informed by two objectives. First, to understand how the chosen governance strategy and structure of two South African BRs have helped to achieve a context appropriate interpretation and actioning of MAB in the landscape. Second, to understand how landscape-level collaborative conservation initiatives support leopard conservation across a multifunctional landscape. This study takes on a mixed methods approach in which qualitative data takes priority and quantitative data is used for support in an embedded multiple-case study design. The sampling techniques used include purposive sampling of strategically targeted participants, i.e. those governing BRs and those leading the collaborative initiative and snowballing thereon. Data collection included a combination of semi-structure interviews (n=20) (the principal collection method), a review of archival, policy and other documents (n=15), and supported by a mixed methods survey questionnaire (n=5). Data were analysed through thematic and content analysis. Findings suggest that organisations working at a landscape-level utilise forms of adaptive co-management and/or governance as a means to being both adaptive and collaborative. Important themes which emerge include cross-sectoral approaches, networks, leadership and trust for building partnerships, shared visions and ‘filling gaps’ left by, or collaborating with the state to fulfil mutually beneficial objectives. This research provides empirical studies on MAB, important for sharing knowledge across the World Network of BRs, meeting the need for further research on collaborative initiatives in social-ecological systems and conservation programmes for large free-roaming carnivores in multifunctional landscapes. , Thesis (MSc) -- Faculty of Science, School of Natural Resource Science & Management, 2024
- Full Text:
- Date Issued: 2024-12
Comparative study of the effect of iloprost on neuroinflammatory changes in c8-b4 microglial cells and murine model of trypanosomiasis
- Authors: Jacobs, Ashleigh
- Date: 2024-12
- Subjects: Neuroimmunology , DNA -- Methylation , Nervous system -- Degeneration
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68895 , vital:77160
- Description: Neurodegenerative conditions significantly impact well-being and quality of life in individuals with major symptoms including mood disorders, cognitive decline, and psychiatric disturbances, often resulting from neuroinflammation triggered by immune responses to bacterial or parasitic infections such as gram-negative bacteria or Human African Trypanosomiasis. Microglia play a crucial role in both neurotoxicity and cellular processes involved in restoring the neural health. Exploring the therapeutic potential of prostacyclin and its analogues in regulating microglia responses to inflammatory insult and treating Trypanosoma brucei (T.b) infection remains an unexplored area. The aim of this study was to assess the potential neuroprotective effects of Iloprost through comparative analysis of neuroinflammatory responses in both microglial cells exposed to lipopolysaccharide (LPS) and mouse brains infected with T.b brucei. In phase I of this study both resting and LPS treated C8-B4 microglial cells were exposed to varying concentrations of Iloprost. The effects of Iloprost on LPS-induced inflammation were analysed using immunofluorescence to detect microglial activation and differentiate between pro and anti-inflammatory phenotypes. Furthermore, pro and anti-inflammatory cytokine secretion was determined using an ELISA, in addition gene expression analysis was carried out using quantitative polymerase chain reaction (qPCR). Also, DNA methylation status of C8-B4 cells exposed to LPS challenge alone or in combination with various concentrations of Iloprost were determined using bisulfite sequencing technique followed by qPCR. In phase II of the study, a total of twenty-four Albino Swiss male mice (8-10 weeks old) were divided into four treatment groups with 6 mice in each group. All treatment groups except the non-infected control were inoculated with the T.b brucei parasite. One group received a single intraperitoneal injection of Diminazene aceturate (4 mg kg-1)while the remaining group received repeated intraperitoneal injections of Iloprost (200 μg kg-1). On day ten of the study, mouse brains were removed on ice using forceps. The hippocampal tissues were dissected out and processed for quantification of gene expression changes in pro and anti-inflammatory cytokines. Overall, the findings of this study indicate that LPS-induced pro-inflammatory cytokine, TNF-α and IL-1β, secretion and gene expression is down-regulated in C8-B4 microglial cells treated with Iloprost. Furthermore, there was a significant up-regulation in the expression of anti-inflammatory genes, particularly ARG-1, CD206, BDNF and CREB in response to Iloprost treatment following LPS-induced inflammation. This study is also the first to confirm M2 microglial polarization with Iloprost treatment in both resting and LPS treated cells. However, hypermethylation at CREB and BDNF promoter regions was observed 24 hours after Iloprost treatment. Additionally, Iloprost reversed hypomethylation at the BDNF promoter region that had been induced by LPS treatment. The rodent model also indicated a downregulation in the pro-inflammatory cytokine, IL-1β, expression and upregulation of BDNF transcription in T.b brucei infected mice treated with repeated doses of Iloprost. In conclusion, determining the immunomodulatory roles of Iloprost in both in vitro and in vivo models of neuroinflammation could assist in the development of alternative therapy for neurodegenerative disease. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Jacobs, Ashleigh
- Date: 2024-12
- Subjects: Neuroimmunology , DNA -- Methylation , Nervous system -- Degeneration
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/68895 , vital:77160
- Description: Neurodegenerative conditions significantly impact well-being and quality of life in individuals with major symptoms including mood disorders, cognitive decline, and psychiatric disturbances, often resulting from neuroinflammation triggered by immune responses to bacterial or parasitic infections such as gram-negative bacteria or Human African Trypanosomiasis. Microglia play a crucial role in both neurotoxicity and cellular processes involved in restoring the neural health. Exploring the therapeutic potential of prostacyclin and its analogues in regulating microglia responses to inflammatory insult and treating Trypanosoma brucei (T.b) infection remains an unexplored area. The aim of this study was to assess the potential neuroprotective effects of Iloprost through comparative analysis of neuroinflammatory responses in both microglial cells exposed to lipopolysaccharide (LPS) and mouse brains infected with T.b brucei. In phase I of this study both resting and LPS treated C8-B4 microglial cells were exposed to varying concentrations of Iloprost. The effects of Iloprost on LPS-induced inflammation were analysed using immunofluorescence to detect microglial activation and differentiate between pro and anti-inflammatory phenotypes. Furthermore, pro and anti-inflammatory cytokine secretion was determined using an ELISA, in addition gene expression analysis was carried out using quantitative polymerase chain reaction (qPCR). Also, DNA methylation status of C8-B4 cells exposed to LPS challenge alone or in combination with various concentrations of Iloprost were determined using bisulfite sequencing technique followed by qPCR. In phase II of the study, a total of twenty-four Albino Swiss male mice (8-10 weeks old) were divided into four treatment groups with 6 mice in each group. All treatment groups except the non-infected control were inoculated with the T.b brucei parasite. One group received a single intraperitoneal injection of Diminazene aceturate (4 mg kg-1)while the remaining group received repeated intraperitoneal injections of Iloprost (200 μg kg-1). On day ten of the study, mouse brains were removed on ice using forceps. The hippocampal tissues were dissected out and processed for quantification of gene expression changes in pro and anti-inflammatory cytokines. Overall, the findings of this study indicate that LPS-induced pro-inflammatory cytokine, TNF-α and IL-1β, secretion and gene expression is down-regulated in C8-B4 microglial cells treated with Iloprost. Furthermore, there was a significant up-regulation in the expression of anti-inflammatory genes, particularly ARG-1, CD206, BDNF and CREB in response to Iloprost treatment following LPS-induced inflammation. This study is also the first to confirm M2 microglial polarization with Iloprost treatment in both resting and LPS treated cells. However, hypermethylation at CREB and BDNF promoter regions was observed 24 hours after Iloprost treatment. Additionally, Iloprost reversed hypomethylation at the BDNF promoter region that had been induced by LPS treatment. The rodent model also indicated a downregulation in the pro-inflammatory cytokine, IL-1β, expression and upregulation of BDNF transcription in T.b brucei infected mice treated with repeated doses of Iloprost. In conclusion, determining the immunomodulatory roles of Iloprost in both in vitro and in vivo models of neuroinflammation could assist in the development of alternative therapy for neurodegenerative disease. , Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 2024
- Full Text:
- Date Issued: 2024-12
Construction material waste control success model for the construction industry
- Authors: Musah, Abdul Razak
- Date: 2024-12
- Subjects: Construction industry -- Materials management , Construction industry -- Management , Construction industry -- Environmental aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68796 , vital:77114
- Description: Economic sustainability and reconstruction in many economies rely heavily on the construction sector and remain strategic tools for governments and industries worldwide. Excessive construction costs and materials waste among some construction companies are rampant in the construction sector. Most of this waste is poorly managed, thus triggering significant project failure problems, leading to the project stakeholders losing the value of their investment. This concern advocate for effective control of construction material to avoid wastage. The primary objective of this study was to develop a Construction Waste Material Control (CWMC) model to be used by construction project professionals throughout the Project Management (PM) phases: initiation, planning, execution, monitoring, control and closure. Significant literature on construction materials waste, Project Management (PM) processes, and project performance regarding poor waste management and project failure was reviewed. This literature review was conducted to identify factors affecting Construction Waste Material Control (CWMC). These factors were identified and defined together with their respective contributions to the model. A quantitative survey instrument (questionnaire) was used to obtain information from construction sector professionals. A total of 213 questionnaire were received and administered for the study. Structural Equation Model (SEM) SEM was used to analyse the questionnaire data, and thereby assisting the research to clearly define latent constructs statistically significant to the (CWMC) model. The finding was that, the factors identified to have impact on CWMC are Project Initiation Phase Tools (PIPTs), Project Planning Phase Tools (PPPTs), project failure/success factors (PFFs), Project Execution Phase Tools (PEPTs), Project Monitoring and Control Phase Tools (PMCPTs), Construction waste material Control (CWMC) policy and Project Management (PM) competence. The supported hypotheses also authenticated these factors. Introducing the model into the construction sector provides another dimension to CWMC. By establishing the aforementioned factors, the model gives construction professionals precise CWMC predictive accuracy. As the model reliability test of 0.734 to 0.972 satisfied the theoretical threshold and goodness of fit of 0.912 of Normed fit index respectively. The model is the first of its kind and contributes significantly to the construction sector’s existing body of knowledge. , Thesis (PhD) -- Faculty of Engineering, the Built Environment, and Technology, School of Built Environment and Civil Engineering, 2024
- Full Text:
- Date Issued: 2024-12
- Authors: Musah, Abdul Razak
- Date: 2024-12
- Subjects: Construction industry -- Materials management , Construction industry -- Management , Construction industry -- Environmental aspects
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/68796 , vital:77114
- Description: Economic sustainability and reconstruction in many economies rely heavily on the construction sector and remain strategic tools for governments and industries worldwide. Excessive construction costs and materials waste among some construction companies are rampant in the construction sector. Most of this waste is poorly managed, thus triggering significant project failure problems, leading to the project stakeholders losing the value of their investment. This concern advocate for effective control of construction material to avoid wastage. The primary objective of this study was to develop a Construction Waste Material Control (CWMC) model to be used by construction project professionals throughout the Project Management (PM) phases: initiation, planning, execution, monitoring, control and closure. Significant literature on construction materials waste, Project Management (PM) processes, and project performance regarding poor waste management and project failure was reviewed. This literature review was conducted to identify factors affecting Construction Waste Material Control (CWMC). These factors were identified and defined together with their respective contributions to the model. A quantitative survey instrument (questionnaire) was used to obtain information from construction sector professionals. A total of 213 questionnaire were received and administered for the study. Structural Equation Model (SEM) SEM was used to analyse the questionnaire data, and thereby assisting the research to clearly define latent constructs statistically significant to the (CWMC) model. The finding was that, the factors identified to have impact on CWMC are Project Initiation Phase Tools (PIPTs), Project Planning Phase Tools (PPPTs), project failure/success factors (PFFs), Project Execution Phase Tools (PEPTs), Project Monitoring and Control Phase Tools (PMCPTs), Construction waste material Control (CWMC) policy and Project Management (PM) competence. The supported hypotheses also authenticated these factors. Introducing the model into the construction sector provides another dimension to CWMC. By establishing the aforementioned factors, the model gives construction professionals precise CWMC predictive accuracy. As the model reliability test of 0.734 to 0.972 satisfied the theoretical threshold and goodness of fit of 0.912 of Normed fit index respectively. The model is the first of its kind and contributes significantly to the construction sector’s existing body of knowledge. , Thesis (PhD) -- Faculty of Engineering, the Built Environment, and Technology, School of Built Environment and Civil Engineering, 2024
- Full Text:
- Date Issued: 2024-12