Towards the provision of relevant, Secondary schools building types through the design of a high schools STEM Centre in Zwide, Nelson Mandela Bay
- Authors: Burger, Sean
- Date: 2020-09
- Subjects: Landscape architecture -- Nelson Mandela Bay
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59194 , vital:60279
- Description: The topic of education in South Africa has always been an area of much debate and contention. The quality of education environments provided in townships in generally always lacking as these facilities are composed of a standardized set of functions. As a result of a budget constraints and poor resources management, most schools lack the required specialised facilities to provide for a full learning experience. With a focus on the development specialised skills in the field of science, mathematics technology and engineering, learners attending these schools are unable to complete with those ore more affluent schools , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2020
- Full Text: false
- Date Issued: 2020-09
- Authors: Burger, Sean
- Date: 2020-09
- Subjects: Landscape architecture -- Nelson Mandela Bay
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59194 , vital:60279
- Description: The topic of education in South Africa has always been an area of much debate and contention. The quality of education environments provided in townships in generally always lacking as these facilities are composed of a standardized set of functions. As a result of a budget constraints and poor resources management, most schools lack the required specialised facilities to provide for a full learning experience. With a focus on the development specialised skills in the field of science, mathematics technology and engineering, learners attending these schools are unable to complete with those ore more affluent schools , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2020
- Full Text: false
- Date Issued: 2020-09
Translating subjugated narratives in post- colonial city texts: The design of a memorial literary resource Centre near Sophiatown, Johannesburg
- Authors: Mazibuko, Nibonge
- Date: 2020-09
- Subjects: Postcolonialism -- Sophia town -- Johannesburg , Apartheid and architecture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59072 , vital:60261
- Description: The reality of a singular narrative is that it at its core it is exclusionary. A singular narrative is undemocratic as it asserts for dominance rather than forbearance. Through the key writings of theorists Henri Lefebvre and Edward Soja the built environment can be seen as a communicative text expressing the higher-level concerns and ideologies of that particular society. In the context of contemporary post-apartheid South African the cities previously entrenched colonial ideologies and systems continue to dominate and to exclude other narratives and expressions, particularly those of previously marginalised voices, from the greater expression of the democratic city text promoting a singular widely accepted narrative. This disparity has been a concern for many architects and built environment professionals who have had to grapple with the question of what is a democratic, authentic and cosmopolitan African identity within the registers of public architecture. The realities are that to ignore this question would be to allow the continual silencing and perpetuation of injustice against those who are marginalised by the residual effects of the apartheid regime. This exclusion from participation within the development of urban environments is dealt with in this treatise from the perspective of physical expression and representation in the exploration of elevating various narratives, stories, typologies for example within the same city text to reflect a more cosmopolitan, democratic narrative. Another area the treatise deals with is the spatial dimension in challenging local level colonial entrenchments of spatial injustice that continue to work to the disadvantage of the urban poor through an understanding of how these are distilled from higher levels. The Sophiatown/Westbury precinct in the western areas of Johannesburg is seen as one such area which continues to suffer from firstly the residual effects of apartheid segregationist planning as well as an embedded physically un-commemorated history which was erased from the physical realm of the city text through demolition during the apartheid regime and replaced with a newbuilt fabric and a new community of people as a stratospheric layer covering what used to be a vibrant, multicultural and hence highly anti-apartheid precinct. The treatise deals with the challenging and un-layering of this entrenched spatial injustice which is a concept defined and qualified in the writing of theorists Henri Lefebvre and Edward Soja. Ideas and themes expressed by Johnathan Alfred Noble on discovering and expressing cosmopolitan identities and narratives within the South African city text are explored to formulate a non-conclusive modus operandi in the scope expressing suppressed and embedded narratives and liberating them into the story and fabric of the built environment as apart of the wider ongoing conversation of redressing the wrongs of the past in rescripting post-colonial urban spaces. The architectural design dovetails from Nobles ideas regarding expression and representation and becomes like a perforated canvas over the site area which allows the emergent spatial and programmatic conditions of the site to interweave with the characteristics and attributes of the old Sophiatown/Westbury precinct to become a dynamic urban catalyst which liberates the legacy of the area into the physical realm of the city. The study was undertaken through desktop research, literature reviews and first-hand observations and analysis within the qualitative research paradigm. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2020
- Full Text:
- Date Issued: 2020-09
- Authors: Mazibuko, Nibonge
- Date: 2020-09
- Subjects: Postcolonialism -- Sophia town -- Johannesburg , Apartheid and architecture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/59072 , vital:60261
- Description: The reality of a singular narrative is that it at its core it is exclusionary. A singular narrative is undemocratic as it asserts for dominance rather than forbearance. Through the key writings of theorists Henri Lefebvre and Edward Soja the built environment can be seen as a communicative text expressing the higher-level concerns and ideologies of that particular society. In the context of contemporary post-apartheid South African the cities previously entrenched colonial ideologies and systems continue to dominate and to exclude other narratives and expressions, particularly those of previously marginalised voices, from the greater expression of the democratic city text promoting a singular widely accepted narrative. This disparity has been a concern for many architects and built environment professionals who have had to grapple with the question of what is a democratic, authentic and cosmopolitan African identity within the registers of public architecture. The realities are that to ignore this question would be to allow the continual silencing and perpetuation of injustice against those who are marginalised by the residual effects of the apartheid regime. This exclusion from participation within the development of urban environments is dealt with in this treatise from the perspective of physical expression and representation in the exploration of elevating various narratives, stories, typologies for example within the same city text to reflect a more cosmopolitan, democratic narrative. Another area the treatise deals with is the spatial dimension in challenging local level colonial entrenchments of spatial injustice that continue to work to the disadvantage of the urban poor through an understanding of how these are distilled from higher levels. The Sophiatown/Westbury precinct in the western areas of Johannesburg is seen as one such area which continues to suffer from firstly the residual effects of apartheid segregationist planning as well as an embedded physically un-commemorated history which was erased from the physical realm of the city text through demolition during the apartheid regime and replaced with a newbuilt fabric and a new community of people as a stratospheric layer covering what used to be a vibrant, multicultural and hence highly anti-apartheid precinct. The treatise deals with the challenging and un-layering of this entrenched spatial injustice which is a concept defined and qualified in the writing of theorists Henri Lefebvre and Edward Soja. Ideas and themes expressed by Johnathan Alfred Noble on discovering and expressing cosmopolitan identities and narratives within the South African city text are explored to formulate a non-conclusive modus operandi in the scope expressing suppressed and embedded narratives and liberating them into the story and fabric of the built environment as apart of the wider ongoing conversation of redressing the wrongs of the past in rescripting post-colonial urban spaces. The architectural design dovetails from Nobles ideas regarding expression and representation and becomes like a perforated canvas over the site area which allows the emergent spatial and programmatic conditions of the site to interweave with the characteristics and attributes of the old Sophiatown/Westbury precinct to become a dynamic urban catalyst which liberates the legacy of the area into the physical realm of the city. The study was undertaken through desktop research, literature reviews and first-hand observations and analysis within the qualitative research paradigm. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2020
- Full Text:
- Date Issued: 2020-09
Investigating cannabinoids and endocannabinoid receptors as drug targets for pain and inflammation
- Authors: Marwarwa, Sinobomi Zamachi
- Date: 2020
- Subjects: Cannabinoids , Cannabinoids Receptors , Inflammation Alternative treatment , Pain Alternative treatment , Drug targeting
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/164468 , vital:41121
- Description: Cannabinoids and the endocannabinoid system have been studied in the past decades but have yet to be fully understood. An insight into interactions that occur between cannabinoid compounds and their receptors is important for understanding the cannabinoids and the endocannabinoid system. Cannabinoids are natural products found in some cannabis plants, and they have similar effects to endocannabinoids, which are chemicals in the body that are involved many aspects of health from appetite, memory, and movement to pain, inflammation and response to cancer. Cannabinoids have a high impact on the treatment of pain and inflammation, they show different antinociceptive mechanisms to existing drugs like opioids, also, they have antimigraine properties better than those achieved by aspirin. The CB1 and CB2 human receptors have been the most studied cannabinoid receptors. In this project, we used a combination of mass-spectrometry to generate plausible chemical fragments and computational techniques to assess the binding of these fragments to these two main CB receptors. CB1 was adapted from the protein data bank (PBD), file 5U09 and the CB2 model was predicted using the hierarchical protocol I-TASSER, starting from the amino acid sequence in UniProt (P34972 CNR2_HUMAN). The proposed active site for CB1 was reported in a publication accompanying the 5U09 PDB model, which was originally generated with a pre-existing ligand in the active site. However, CB2 had to be built from a homology model and the active site determined using a combination of I-TASSER, Maestro, and CASTp the more favourable binding energies were determined by CASTp, leading to the use of the CASTp coordinates as default for docking in the CB2 human receptor. The molecular docking of cannabinoids THC, CBD, CBDV, CBG and CBN on both the CB1 and CB2 proteins was performed to identify the amino acids that interact with these compounds at their active sites. This would provide a guide to a future fragment-based drug discovery (FBDD) synthesis project. The docking in this work showed adequate accuracy with binding energies between -8.23 kcal/mol and -9.97 kcal/mol for CB1 and between -6.78 kcal/mol and -7.74 kcal/mol for CB2. An observation made was that binding energies of the CB1 human receptor docking were higher than those of the CB2 human receptor, which could support the widely held belief that CB1 is more important in cannabinoid interactions. The cannabinoids were then subjected to collision-induced dissociation to produce fragment structures predicted in chapter 2. These hypothetical fragments were docked in the CB1 and CB2 human receptor, the general trend again being the binding energies for the CB1 receptor was again around 10% higher than those of the CB2 receptor. As expected, larger fragments tended to have better binding, with the fragment proposed from m/z 259 with binding energies -9.62 kcal/mol in CB1 and -6.26 kcal/mol. Those fragments with significant lipophilic side chains or some aromatic moiety also showed good binding or around -6.00 kcal/mol, similar to the intact cannabinoids. In our case, this fragment was proposed from m/z 223 with binding energies -7.71 kcal/mol in CB1 and -6.5 kcal/mol in CB2. The results from the fragment dockings were favourable in that they have binding affinities lower than -6.0 kcal/mol which is good enough for the structures to be leads in the creation of fragment libraries. The docking was performed with Autodock 1.5.6 and data visualization with a discovery studio. , Thesis (MSc) -- Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020
- Authors: Marwarwa, Sinobomi Zamachi
- Date: 2020
- Subjects: Cannabinoids , Cannabinoids Receptors , Inflammation Alternative treatment , Pain Alternative treatment , Drug targeting
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/164468 , vital:41121
- Description: Cannabinoids and the endocannabinoid system have been studied in the past decades but have yet to be fully understood. An insight into interactions that occur between cannabinoid compounds and their receptors is important for understanding the cannabinoids and the endocannabinoid system. Cannabinoids are natural products found in some cannabis plants, and they have similar effects to endocannabinoids, which are chemicals in the body that are involved many aspects of health from appetite, memory, and movement to pain, inflammation and response to cancer. Cannabinoids have a high impact on the treatment of pain and inflammation, they show different antinociceptive mechanisms to existing drugs like opioids, also, they have antimigraine properties better than those achieved by aspirin. The CB1 and CB2 human receptors have been the most studied cannabinoid receptors. In this project, we used a combination of mass-spectrometry to generate plausible chemical fragments and computational techniques to assess the binding of these fragments to these two main CB receptors. CB1 was adapted from the protein data bank (PBD), file 5U09 and the CB2 model was predicted using the hierarchical protocol I-TASSER, starting from the amino acid sequence in UniProt (P34972 CNR2_HUMAN). The proposed active site for CB1 was reported in a publication accompanying the 5U09 PDB model, which was originally generated with a pre-existing ligand in the active site. However, CB2 had to be built from a homology model and the active site determined using a combination of I-TASSER, Maestro, and CASTp the more favourable binding energies were determined by CASTp, leading to the use of the CASTp coordinates as default for docking in the CB2 human receptor. The molecular docking of cannabinoids THC, CBD, CBDV, CBG and CBN on both the CB1 and CB2 proteins was performed to identify the amino acids that interact with these compounds at their active sites. This would provide a guide to a future fragment-based drug discovery (FBDD) synthesis project. The docking in this work showed adequate accuracy with binding energies between -8.23 kcal/mol and -9.97 kcal/mol for CB1 and between -6.78 kcal/mol and -7.74 kcal/mol for CB2. An observation made was that binding energies of the CB1 human receptor docking were higher than those of the CB2 human receptor, which could support the widely held belief that CB1 is more important in cannabinoid interactions. The cannabinoids were then subjected to collision-induced dissociation to produce fragment structures predicted in chapter 2. These hypothetical fragments were docked in the CB1 and CB2 human receptor, the general trend again being the binding energies for the CB1 receptor was again around 10% higher than those of the CB2 receptor. As expected, larger fragments tended to have better binding, with the fragment proposed from m/z 259 with binding energies -9.62 kcal/mol in CB1 and -6.26 kcal/mol. Those fragments with significant lipophilic side chains or some aromatic moiety also showed good binding or around -6.00 kcal/mol, similar to the intact cannabinoids. In our case, this fragment was proposed from m/z 223 with binding energies -7.71 kcal/mol in CB1 and -6.5 kcal/mol in CB2. The results from the fragment dockings were favourable in that they have binding affinities lower than -6.0 kcal/mol which is good enough for the structures to be leads in the creation of fragment libraries. The docking was performed with Autodock 1.5.6 and data visualization with a discovery studio. , Thesis (MSc) -- Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020