Effects of dietary inclusion of Moringa oleifera leaf meal on growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
Effects of feeding Moringa Oleifera leaf meal as an additive on growth performance of chicken, physico-chemical shelf-life indicators, fatty acid profiles and lipid oxidation of broiler meat
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
Antioxidant activity and the quality of meat from goats and broilers supplemented with Moringa (Moringa Oleifera) leaves
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
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