Effects of BT Maize (MON810) crop and its residues on selected soil biological properties and N and P release in a sandy loam soil from Alice, Eastern Cape, South Africa
- Authors: Landzela, Besule
- Date: 2013
- Subjects: Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11870 , http://hdl.handle.net/10353/d1007542 , Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Description: There are apprehensions that genetic modification of maize with Bacillus thuringiensis (Bt) may have negative effects on soil biodiversity, ecosystem processes and functions. This study aimed at determining the effect of Bt maize crop, Bt maize residues and its genetic modification on microbial biomass carbon (MBC), selected enzyme activities, vesicular arbuscular mycorrhizal (VAM) fungi and N and P release patterns. The study was conducted under field, glasshouse and laboratory conditions. In 2010/2011 season, four maize cultivars; DKC 61-25B (Bt), DKC 61-24 (non-Bt), PAN 6Q-321B (Bt) and PAN6777 (non-Bt) were planted. Determination of MBC, enzyme activities and fungal spore count was done at 42, 70, and 105 days after planting (DAP). A loam soil amended with Bt or non-Bt maize leaf residues from a study of 2009/2010 season was incubated to investigate effects of Bt maize residues on MBC and soil enzyme activities. Leaf residues of Bt and non-Bt maize cultivars (DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777) were used and soil without residues was used as a control. Samples were collected at 7, 28 and 56 days of incubation (DOI). An incubation study was also carried out in the laboratory to determine the effect of Bt maize residues (i.e. leaf, stem and root) and its genetic modification on N and P release patterns. Residues of DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777and soil without residues as a control were incubated in the laboratory. After destructive sampling at 0, 7, 14, 28, and 56 DOI, N in the form of NH4-N and NO3-N and P mineralisation were determined. Amendment of soil with residues enhanced MBC (p < 0.05) at all the sampling dates. For example MBC increased from 95 in the control to 146.3 mg/kg in the DKC 61-25B treatment at the end of the glasshouse trial. In the field DKC 61-25B had 9.1 mg/kg greater MBC than DKC 61-24, while PAN 6Q-321B had 23.9 mg/kg more MBC than PAN6777 at the end of the trial. However, no differences (p < 0.05) were observed in enzyme activities under field and glasshouse conditions except for dehydrogenase that had greater activity where DKC 61-25B and PAN 6777 were grown. There were no differences between the type of residues (Bt and non-Bt) on enzyme activities tested. However, differences were observed among the sampling dates. No effects of Bt maize crop on fungal spore count were observed. Similarly no differences were observed in leaf, stem and root tissues composition between Bt and non-Bt maize cultivars. Net N and P mineralisation from Bt maize cultivars did not differ from that of non-Bt maize cultivars. However, differences were observed among the cultivars. The results of this study suggested that Bt maize with Bt MON810 event can be grown in the central region of the Eastern Cape (EC), South Africa without affecting MBC, soil enzyme activities, VAM, and release of N and P nutrients from its residues.
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- Date Issued: 2013
Cattle manure, scalping and soil wetness effects on some physical properties of a hardsetting soil and associated early maize growth
- Authors: Nciizah, Adornis Dakarai
- Date: 2011
- Subjects: Soil formation , Crops and soils , Manures , Soil mechanics , Soil moisture , Soil stabilization , Soil penetration test
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11861 , http://hdl.handle.net/10353/349 , Soil formation , Crops and soils , Manures , Soil mechanics , Soil moisture , Soil stabilization , Soil penetration test
- Description: Most soils in the Eastern Cape Province, South Africa are shallow and are low in organic matter. Therefore these soils are structurally fragile and highly susceptible to inherent degradative processes like hardsetting. The objective of this study was to determine the effect of cattle manure, scalping and soil wetness on aggregate stability, penetration resistance and early maize growth in hardsetting soils. Glasshouse and field studies were conducted to determine the effect of cattle manure on aggregate stability and penetration resistance of freshly exposed topsoils by scalping at 0, 10 and 20 cm depths. In the glasshouse cattle manure was applied at 0 and 20 Mg/ha and matric suction was kept at ~ 30 and ~ 400 kPa; contrasting high and low soil wetness. Three soils were put in pots and arranged in a randomized complete block 3 2 2 factorial design. The field study was done at the University of Fort Hare research farm and the treatments were arranged in a split-plot complete randomized design with three replications. Scalping treatment was the main plot whilst the quantity of the cattle manure applied was the sub plot. Cattle manure increased mean weight diameter (MWD) by between 48% and 71% under glasshouse and between 18% and 33% under field conditions, depending on the soil wetting rate. Cattle manure reduced MWD when the soil under field condition was subjected to mechanical shaking. Soil penetration resistance decreased linearly, with increasing soil wetness but it rapidly increased with increase in matric suction up to ~200 kPa and thereafter the rate of increase reduced. In the glasshouse, all treatments had no significant effects on shoot dry weight but low matric suction increased root dry weight by 133%. Interaction of cattle manure and low matric suction reduced shoot length by 6%, shoot fresh weight by 25%, root surface area by 36%, root length by 5% and root fresh weight by 29% compared to the control. In contrast, application of cattle manure and high matric suction increased shoot length by 37%, shoot fresh weight by 136%, root surface area by 159%, root length by 94% and root fresh weight by 119%. In the field, cattle manure application increased root length density and shoot dry matter by 26% and 30% respectively. Cattle manure improved the stability of aggregates of the hardsetting soil under rapid or slow water intake conditions experienced during rainfall or irrigation. However, under field conditions cattle manure acted as a deflocculant and decreased the stability of aggregates when mechanical stress was applied. The effectiveness of cattle manure in improving maize growth in hardsetting soils was determined by matric suction.
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- Date Issued: 2011
Effect of BT maize on earthworm activity, silage quality and residue decomposition in the central Eastern Cape
- Authors: Kamota, Agathar
- Date: 2011
- Subjects: Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11863 , http://hdl.handle.net/10353/413 , Corn -- South Africa -- Eastern Cape , Corn -- Silage , Bacillus thuringiensis , Earthworm culture , Biomass , Plant proteins
- Description: There are concerns that genetic modification of maize with Bacillus thuringiensis may influence its structural and chemical composition which, together with the Cry1Ab proteins, may affect agro-ecosystem processes and feed quality. This study was aimed at investigating the expression of Cry1Ab protein in leaves, stems and roots of Bt maize and the effect of genetic modification (MON810) on activity of earthworms, silage quality and decomposition of residues in the field. In 2009/10 four maize cultivars; DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777 were planted. Expression of Cry1Ab in leaves, stems and roots was analyzed at 6, 8, 10 and 12 weeks after planting (WAP). Earthworms were also sampled from the same treatments at 6, 9 and 18 WAP. Two silage experiments were conducted using maize cultivars, DKC80-12B and DKC80-10 produced in the 2008/09 season and DKC61-25B, DKC61-24, PAN6Q-321B and PAN6777, produced in 2009/10. The silage was sampled at 0, 2, 4, 8, 15 and 42 days in 2008/09 and 0, 8 and 42 days in 2009/10 and analyzed for Ash Free Dry Matter, Crude Fiber, Neutral Detergent Fiber, Acid Detergent Fiber, Acid Detergent Lignin, Crude Protein and Total Digestible Nutrients. Two litter-bag decomposition studies were also carried out (i) in 2008 (surface applied) using maize cultivars DKC80-12B, DKC80-10 and DKC6-125 residues and (ii) in 2009 (soil incorporated) using DKC75-15B, CRN3505, PAN6Q-321B v and PAN6Q-121. Ash-free dry matter and Cry1Ab protein were measured throughout the incubation time. There were no differences between DKC61-25B and PAN6Q-321B in terms of expression of Cry1Ab in leaves, stems and roots over time. The Cry1Ab expression levels were in decreasing order: leaves > stems > roots. No effects of Bt maize on earthworm numbers and biomass were observed. There were no differences in all silages parameters except NDF and ADF, which were higher in the Bt maize silage than that of the non-Bt maize from the 2008/09 season. The Cry1Ab levels were essentially not reduced during ensiling. The maize residues (both Bt and non-Bt maize) degraded to similar levels, either when surface-applied or incorporated into soil but soil-incorporated residues decomposed faster than surface-applied ones. Cry1Ab degraded as the plant matrix decomposed. The findings suggested that maize genetically modified with the Bt MON810 event can be grown in the Central Eastern Cape without affecting earthworm numbers and biomass, silage quality and decomposition of maize residues.
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- Date Issued: 2011
Effects of genetically modified maize (MON810) and its residues on the functional diversity of microorganisms in two South African soils
- Authors: Puta, Usanda
- Date: 2011
- Subjects: Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11250 , http://hdl.handle.net/10353/419 , Genetically modified foods -- South Africa , Transgenic plants -- South Africa , Crops -- Genetic engineering -- South Africa , Soil microbiology -- South Africa , Microorganisms , Microbial ecology , Rhizosphere -- Microbiology , Vesicular-arbuscular mycorrhizas , Corn -- South Africa
- Description: Genetically modified (GM) crops are commercially cultivated worldwide but there are concerns on their possible negative impacts on soil biodiversity. A glasshouse study was conducted to determine effects of Bt maize residues on soil microbial diversity. Residues of Bt maize (PAN 6Q-308B) and non-Bt maize (PAN 6Q-121) were incorporated into the soil and corresponding maize seeds planted. The treatments were replicated three times. Fertilizer and water application were similar for both treatments. Rhizosphere and bulk soil was destructively sampled from each treatment and analyzed for microbial community level physiological profiles using Biolog plates with 31 different carbon substrates. Absorbance in the Biolog plates was recorded after 72 h of incubation at 20oC. Arbuscular mycorrhizal fungi spore counts were also determined. Field studies were conducted at the University of Free State and University of Fort Hare Research Farms to determine the effects of growing Bt maize on soil microbial diversity. One Bt maize cultivar (PAN6Q-308B) and non-Bt maize (PAN6Q-121) were grown in a paired experiment at University of Free State farm, while two Bt maize (DKC61-25B and PAN6Q-321B) and their near-isogenic non-Bt maize lines (DKC61-24 and PAN6777) were grown in a randomized complete block design with three replicates. Fertilization, weed control and water application, were similar for both Bt maize cultivars and their non-Bt maize counterparts. Rhizosphere soil samples were collected by uprooting whole plants and collecting the soil attached to the roots. The samples were analysed for microbial diversity and for arbuscular mycorrhizae fungal spore counts. Principal component analysis showed that soil microbial diversity was affected more by sampling time whereas genetic modification had minimal effects. Presence of residues also increased the diversity of microorganisms. Mycorrhizal fungal spores were not affected by the presence of Bt maize residues. Growing Bt maize had no effect on the soil microbial diversity in the rhizosphere.
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- Date Issued: 2011
Evaluating summer cover crop species and management strategies for rainfed maize based cropping systems in the central region of the Eastern Cape Province of South Africa
- Authors: Ganyani, Lloyd Munashe
- Date: 2011
- Subjects: No-tillage , Sustainable agriculture -- South Africa -- Eastern Cape , Rain and rainfall -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crops and climate -- South Africa -- Eastern Cape , Agricultural systems -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11865 , http://hdl.handle.net/10353/373 , No-tillage , Sustainable agriculture -- South Africa -- Eastern Cape , Rain and rainfall -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crops and climate -- South Africa -- Eastern Cape , Agricultural systems -- South Africa -- Eastern Cape
- Description: The overall objective of the whole study was to assess whether conservation agriculture (CA) systems can work in the Eastern Cape Province (EC). The CA systems were engaged through cover cropping to address land degradation problems by emphasizing high biomass production in order to realize short term benefits such as moisture conservation, weed suppression and soil fertility benefits under rainfed conditions in the central region of the Eastern Cape province. Since rainfall is the most limiting factor to crop production in the EC, a within season rainfall distribution analysis was conducted to expose the quality of the season (onset, end and duration) and hence the feasibility of CA systems to guide agronomic decisions by farmers in EC. To assess season parameters, thirty four years of daily rainfall was collected from the University of Fort Hare Research station and used to conduct the rainy pentad (5 day rainfall totals) analysis and the daily rainfall analysis using INSTAT software programme. Based on the pentad analysis, results showed that Alice does not have a rainy season in 1 out of 2 years (50% probability) but has one in 1 out of 4 years (25% probability level). This criterion proved to be harsher and conservative when compared to the daily rainfall approach which is more precise in measuring trends on season parameters. The daily rainfall analysis indicated a 65% feasibility for the dry land cropping systems in the EC. The pentad analysis however was effective in illustrating seasonality and it showed that the wet season begins on the 1st of November, ending on the 22nd of March lasting for 140 days. Though the season duration appeared too long, the existence of dry spells during critical growth stages adversely affects the quality of the season. The daily rainfall analysis also managed to derive a signal which can guide planting decisions. For planting to be successful, this analysis determined that 20 mm of rain should be received in two consecutive days after the 1st of November. A screening trial for cover crop biomass production and weed suppression was conducted on-station Fort Hare Research Farm (32°46' S and 26° 50' E), and Msobombvu village (MSBV) (32°44' S, and 26° 55' E) over two seasons (2007/08 and 2008/09). Six summer cover crops i.e. cowpea (Vigna unguiculata), dolichos lablab (Dolichos argenteus), sunnhemp (Crotalaria juncea), buckwheat (Fagopyrum sagittatum), forage sorghum (Sorghum bicolor) and sunflower (Helianthus annus) were evaluated for biomass yield, and weed suppression. Decomposition rates, moisture conservation and residual effects of these cover crops on the succeeding main crop were also evaluated under dryland conditions. The screening trial was laid in randomized complete block design replicated three times. Forage sorghum (Sorghum bicolor) and sunflower (Helianthus annus) were identified as high biomass producers and their dry matter yields ranged from 8 -12 t ha-1. These cover crops can be useful in generating high biomass in rainfed cropping systems in the EC. Other cover crops produced 3 - 4 t ha-1 of biomass which fell short of the 6 t ha-1 expected benchmark. However, these biomass yields were important in weed management since all cover crop species showed a similar degree of weed suppression which surpassed the weed fallow treatment. As dead mulches, the cover crops failed to show residual moisture conservation and weed control benefits for the succeeding maize crop mainly because of poor residue persistence, and low harvestable fallow rainfall. Buckwheat (Fagopyrum esculentum), was selected for further investigations in a follow up trial on station in 2008/09 season because of its weed smothering qualities, suitability to short cycle rotations, and possible allelopathic properties. The trial aimed at finding weed and cost effective management options of buckwheat that are none detrimental to the succeeding maize crop. Results showed that cropping systems where buckwheat is followed by a main crop may not work as they are unprofitable with respect to R100 rand invested. Though perceived to have allelopathic properties, buckwheat failed to demonstrate the possibilities of allelopathic action against weeds. Intercropping trial was conducted on-station in 2007/8-2008/09 seasons to try and find better ways of fitting legume cover crops into maize based cropping systems without compromising production of staple cereals on limited landholdings. The trials evaluated three factors in factorial combination, cover crop planting date, intercropping strategy, and cover crop species. The trial was laid as 2 x 2 x 3 factorial arranged in a split-split plot design. The main plot factor was cover crop planting date, cover crops simultaneously planted with maize and cover crop planted two weeks after planting maize (DKC 61-25). The sub-plot factor was intercropping strategy, strip intercropping and betweenrow intercropping. The sub-sub-plot factor was cover crop species, Dolichos lablab (Dolichos argenteus (Highworth), and Cowpea Vigna ungiculata (Agrinawa) plus control plots of sole maize. Results showed that same time planting of leguminous cover crops with maize using the in-between row intercropping patterns can derive appreciable system biomass (maize/cover crop) yields, utilize land efficiently whilst getting favourable maize grain yield. Based on the rainfall analysis, results showed that the probability of success when relay seeding cover crops after two weeks into standing maize is low (15% chances of success). This suggests that relay intercropping strategies would not work due to the unavailability of a good quality season.
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- Date Issued: 2011
Evaluation of cover crop species for biomass production, weed suppression and maize yields under irrigation in the Eastern Cape Province, South Africa
- Authors: Musunda, Bothwell Zvidzai
- Date: 2010
- Subjects: Cover crops , Biomass energy -- South Africa -- Eastern Cape , No-tillage , Conservation of natural resources -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape , Agricultural systems -- South Africa -- Eastern Cape , Weeds
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11867 , http://hdl.handle.net/10353/347 , Cover crops , Biomass energy -- South Africa -- Eastern Cape , No-tillage , Conservation of natural resources -- South Africa -- Eastern Cape , Agriculture -- South Africa -- Eastern Cape , Agricultural systems -- South Africa -- Eastern Cape , Weeds
- Description: Achieving high biomass yields of cover crops has been a challenge to the success of Conservation Agriculture (CA) practices in the Eastern Cape (EC). A study was conducted to evaluate strategies for optimizing cover crop biomass production. Trials were carried out to screen summer and winter cover crops, as well as evaluate intercropping patterns and planting dates for biomass, weed suppression and subsequent maize yield under irrigation. Four summer legume cover crop species were evaluated under a Randomised Complete Block Design (RCBD) design. The cover crops were fertilized with 13.34 kg ha-1 of N, 20 kg ha-1 P and 26.66 kg ha-1 K. In the 2008/09 summer season a maize crop was superimposed on the 2007/08 screening trial under no-till. The crop was fertilized with 60 kg ha-1 of N. An intercropping trial was conducted over two seasons as a way of investigating the best way of incorporating cover crops into farmers cropping systems. This was done bearing in mind the limitation of resources such as land. The trial evaluated 3 factors laid as a 2 x 2 x 3 factorial arranged in a split-plot design. The main factor was cover crop planting date (planting at maize planting or 2 weeks after maize planting). The sub plot factor was intercropping pattern (strip intercropping and between row intercropping). A trial was also conducted to evaluate the effect of planting date (End of April and mid May) and four winter legume cover crop species on cover crop biomass, weed suppression and maize grain yield. The experiment was laid out as a Randomised Complete Block Design (RCBD) replicated 3 times. In the subsequent summer season a maize crop was superimposed on the winter trial to test the residual effects of the cover crop species. Another study was conducted to evaluate winter cereal cover crop species for biomass accumulation, weed suppression and subsequent maize grain yield. The cover crops as well as a weedy fallow control plot treatments were laid out as a Randomised Complete Block Design replicated 3 times. In the subsequent summer season a maize crop was superimposed on the site under no-till to evaluate the residual effect of the cover crops on maize. The results showed sunhemp, cowpea and lablab as the best cover crops with high biomass and weed suppression whilst mucuna was the least. Sunhemp consistently yielded higher cover biomass averaging 11200 kg ha-1 over the two seasons whilst mucuna had a consistently lowest average biomass yield of 4050 kg ha-1. These cover crops were above the critical 6 t ha-1 for effective weed suppression. There was a significant (p<0.01) relationship of cover crop dry weight and weed dry weight in both seasons. Subsequent maize grain yield was significantly higher in the sunhemp plots (64.2 %) than the weedy fallow plot. Mucuna, lablab and cowpea had maize grain yield increases of 16.6%, 33% and 43.2% respectively. Intercropping cover crops at maize planting yielded higher cover crop dry weights than a delay in intercropping cover crops. A delay in intercropping resulted in significantly higher average maize grain yield of 4700 kg ha-1 compared to intercropping at maize planting (3800 kg ha-1) and sole maize (4300 kg ha-1) over the two seasons. Strip intercropping also yielded higher (5000 kg ha- 1) average maize grain yield compared to row intercropping (3600 kg ha-1) and sole maize (4300 kg ha-1). There was a significant (p<0.05) relationship between cover crop dry weight in the 2007/08 season and maize grain yield in the 2008/09 season. Early planting grazing vetch gave the highest biomass yield of 8100 kg ha-1 whilst early planted red clover had the lowest biomass of 635 kg ha-1. Low weed dry weights were also obtained from the early planted grazing vetch as opposed to the other treatments. There was a significant (p<0.001) relationship of cover crop dry weight and weed dry weight. In the subsequent 2008/09 summer season early planted grazing vetch had the highest maize yield of 7500 kg ha-1 which was 56.3 % more than the weedy fallow plot had 4800 kg ha-1. The weedy fallow plot also had high weed infestation than the cover crop plots. There were significant (p<0.01) relationships between cover crop dry weight and maize grain yield, winter weed dry weight and maize grain yield and summer weed dry weight and maize grain yield. The results also showed triticale (13900 kg ha-1) as the best winter cover crop for biomass production. Italian ryegrass (6500 kg ha-1) produced the least amount of biomass. In The subsequent maize crop white oats gave highest maize grain yield (6369 kg ha-1) which was 33 % more than the weedy fallow plot (4784 kg ha- 1). There were also significant (p< 0.01) relationships of maize grain yield and winter weed dry weight, maize grain yield and summer growing weeds. The various studies demonstrated that there is opportunity for high biomass production under small scale farmers irrigated conditions using cover crops both in winter and summer. Best bet cover crops were sunhemp, cowpea and lablab for summer and triticale, white oats, barley, Italian ryegrass and grazing vetch for winter. Cover crops can also be incorporated into farmers cropping systems as sole crops or intercrops within the maize based cropping systems. Strip intercropping can be used by farmers as a way of introducing cover crops. Critical to achievement of high biomass is the time of planting cover crops with high biomass when planting is done early. A 2 week delay in strip intercropping cover crop into maize can be used as a way of incorporating cover crops into farmers cropping systems with minimal maize yield reduction.
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- Date Issued: 2010
Manure production and nutrient management in pasture-based dairy production systems
- Authors: Svinurai, Walter
- Date: 2010
- Subjects: Manures , Nitrogen -- Metabolism , Aloe -- South Africa , Pine bark , Pastures , Dairy cattle -- Nutrition , Dairy cattle -- Management , Dairy cattle -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11812 , http://hdl.handle.net/10353/475 , Manures , Nitrogen -- Metabolism , Aloe -- South Africa , Pine bark , Pastures , Dairy cattle -- Nutrition , Dairy cattle -- Management , Dairy cattle -- Feeding and feeds
- Description: Manure production and nutrient management in pasture-based dairy production systems by Walter Svinurai The effect of dietary treatments on nutrient excretion was determined and an attempt to improving the retention of excreted nitrogen in stored manure using Aloe ferox leaf gel (AFLG) and pine bark was conducted at a pasture-based dairy farm. The animal mass-balance method was used to determine nutrient excretion by cows fed on three dietary treatments, Lolium perrene-based treatment (LP), Lolium multiforium-based treatment (LM) and Lolium perenne- Trifolium repens -based treatment (LTF). In separate experiments, slurry from dairy cows fed LM was amended with AFLG and pine bark at concentrations of 25, 50, 75 and 100 g/l and stored under anaerobic conditions for 16 days. The highest and lowest excretions of N, P and K were observed in LM and LP treatments, respectively. Increasing dietary N improved K and N retention in milk and, consequently increased milk urea nitrogen (MUN) and N excretion (P < 0.05). The concentrations of AFLG affected N and P retention in manure (P < 0.05). The highest retention in total Kjeldahl nitrogen (TKN) of the initial was 42% AFLG at 25g/l inclusion rate. Ammonium nitrogen (AMN) concentration increased significantly due to the interactive effect of AFLG inclusion rate and time. Pine bark powder significantly improved N and P retention in manure at all concentrations. The retention in TKN was higher (P < 0.05) at 25g/l pine bark powder than other concentrations. The interaction effect of inclusion rate and time increased iii AMN (P < 0.05). Dietary treatments significantly affected nutrient excretion, and AFLG and pine bark considerably improved N retention in stored slurry. Findings from the filed trial suggest the need for more attention on managing dietary nutrients in the post-rainy and cool-dry season when growth of pasture influenced choice of dietary treatments that led to high nutrient excretion. Field simulation of the additives to determine their efficacy and environmental hazards was recommended.
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- Date Issued: 2010