A critical appraisal of Saule’s portrayal of women as Amadelakufa (resilient)
- Authors: Molubo, Rendani
- Date: 2020
- Subjects: Women in literature , Feminism -- Africa , Saule, N -- Vuleka Mhlaba , Saule, N -- Inkululeko Isentabeni , Saule, N -- Libambe Lingatshoni , Xhosa fiction -- History and criticism
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/143982 , vital:38300
- Description: This study seeks to explore how Professor Ncedile Saule’s works Vuleka Mhlaba (2006), Inkululeko Isentabeni (2010) and Libambe Lingatshoni (2017), depict female characters to reveal the inner resilience women embody. It is important to portray females as bold, intelligent and courageous beings as African women are subjected to major patriarchal myths. South Africa prides itself with a progressive Constitution and Bill of Rights that speaks of gender equality and the liberation of all persons. However, in practice women still live under patriarchal bondages and their efforts are side-lined, thus, the essentiality of writings that contributes towards the empowerment of women through displaying their resilience. Women in literature are mostly depicted as subordinate, submissive and emotional. At times they are portrayed as victims. They are very seldom portrayed as heroes or independent beings. They are constantly placed behind a man instead of in front of a man. This research aims to display women as heroes in their own capacity, without having to be dimmed by a man. The researcher decided to utilise writings of a male author so as to provide a different perspective from the norm that most male authors are known to write about in issues pertaining female characters. This is also deliberately done to encourage more male writers to change the mythical narration about women and start admiring their contributions without having them shadow any man. The projection of female resilience through the gaze of a male author gives hope that there are men who see and admire women’s efforts. It also indicates that men realise now that African feminisms are not against men and instead advocate for equality between men and women and recognise the greatness that could come out of a partnership between the two genders. Women’s behaviour and characteristics are mostly socially constructed, hence the need to exhibit the boldness that black women possess, instead of them only being utilised as nurturing sources and household caretakers. One of the purposes of this research study is to illustrate the resilience that women possess and analyse the forms of torment that women in Saule’s chosen novels have experienced and how their experience engenders resilience and a determination to succeed. The women in Saule’s three novels selected for this study break the traditional status quo. The approach that this paper will utilize is that of African Feminisms, focusing on Walker’s Womanism, Ogunyemi’s Africana Womanism, and Stiwanism as the theoretical approaches. This is because African Feminisms concentrates on the issues related to gender, race and class that African women across spectrums face.
- Full Text:
- Date Issued: 2020
- Authors: Molubo, Rendani
- Date: 2020
- Subjects: Women in literature , Feminism -- Africa , Saule, N -- Vuleka Mhlaba , Saule, N -- Inkululeko Isentabeni , Saule, N -- Libambe Lingatshoni , Xhosa fiction -- History and criticism
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/143982 , vital:38300
- Description: This study seeks to explore how Professor Ncedile Saule’s works Vuleka Mhlaba (2006), Inkululeko Isentabeni (2010) and Libambe Lingatshoni (2017), depict female characters to reveal the inner resilience women embody. It is important to portray females as bold, intelligent and courageous beings as African women are subjected to major patriarchal myths. South Africa prides itself with a progressive Constitution and Bill of Rights that speaks of gender equality and the liberation of all persons. However, in practice women still live under patriarchal bondages and their efforts are side-lined, thus, the essentiality of writings that contributes towards the empowerment of women through displaying their resilience. Women in literature are mostly depicted as subordinate, submissive and emotional. At times they are portrayed as victims. They are very seldom portrayed as heroes or independent beings. They are constantly placed behind a man instead of in front of a man. This research aims to display women as heroes in their own capacity, without having to be dimmed by a man. The researcher decided to utilise writings of a male author so as to provide a different perspective from the norm that most male authors are known to write about in issues pertaining female characters. This is also deliberately done to encourage more male writers to change the mythical narration about women and start admiring their contributions without having them shadow any man. The projection of female resilience through the gaze of a male author gives hope that there are men who see and admire women’s efforts. It also indicates that men realise now that African feminisms are not against men and instead advocate for equality between men and women and recognise the greatness that could come out of a partnership between the two genders. Women’s behaviour and characteristics are mostly socially constructed, hence the need to exhibit the boldness that black women possess, instead of them only being utilised as nurturing sources and household caretakers. One of the purposes of this research study is to illustrate the resilience that women possess and analyse the forms of torment that women in Saule’s chosen novels have experienced and how their experience engenders resilience and a determination to succeed. The women in Saule’s three novels selected for this study break the traditional status quo. The approach that this paper will utilize is that of African Feminisms, focusing on Walker’s Womanism, Ogunyemi’s Africana Womanism, and Stiwanism as the theoretical approaches. This is because African Feminisms concentrates on the issues related to gender, race and class that African women across spectrums face.
- Full Text:
- Date Issued: 2020
A Critical Evaluation of Validation Practices in the Forensic Acquisition of Digital Evidence in South Africa
- Jordaan, Jason, Bradshaw, Karen L
- Authors: Jordaan, Jason , Bradshaw, Karen L
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/440174 , vital:73754 , ISBN 9783030660390 , https://doi.org/10.1007/978-3-030-66039-0_9
- Description: Accepted digital forensics practice requires the tools used in the forensic acquisition of digital evidence to be validated, meaning that the tools perform as intended. In terms of Sect. 15 of the Electronic Communications and Transactions Act 25 of 2002 in South Africa, validation would contribute to the reliability of the digital evidence. A sample of digital forensic practitioners from South Africa was studied to determine to what extent they make use of validated forensic tools during the acquisition process, and how these tools are proven to be validated. The research identified significant concerns, with no validation done, or no proof of validation done, bringing into question the reliability of the digital evidence in court. It is concerning that the justice system itself is not picking this up, meaning that potentially unreliable digital evidence is used in court.
- Full Text:
- Date Issued: 2020
- Authors: Jordaan, Jason , Bradshaw, Karen L
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/440174 , vital:73754 , ISBN 9783030660390 , https://doi.org/10.1007/978-3-030-66039-0_9
- Description: Accepted digital forensics practice requires the tools used in the forensic acquisition of digital evidence to be validated, meaning that the tools perform as intended. In terms of Sect. 15 of the Electronic Communications and Transactions Act 25 of 2002 in South Africa, validation would contribute to the reliability of the digital evidence. A sample of digital forensic practitioners from South Africa was studied to determine to what extent they make use of validated forensic tools during the acquisition process, and how these tools are proven to be validated. The research identified significant concerns, with no validation done, or no proof of validation done, bringing into question the reliability of the digital evidence in court. It is concerning that the justice system itself is not picking this up, meaning that potentially unreliable digital evidence is used in court.
- Full Text:
- Date Issued: 2020
A critical investigation of leadership in a Technical, Vocational Education and Training college in the Eastern Cape
- Authors: Chagi, Nonkonzo
- Date: 2020
- Subjects: Technical education -- South Africa , Vocational education -- South Africa , Educational leadership -- South Africa , Postsecondary education -- South Africa -- Administration
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/140045 , vital:37827
- Description: Far-reaching reforms of the TVET college system – including a merger and frequent revisioning of the colleges’ role in the educational landscape of South Africa – have focused renewed attention on this sector. The fact that the sector has been plagued by poor performance – even to the extent that several colleges have been placed under administration – suggests problems at the level of leadership. This study sought to explore key role-players’ understanding of the leadership and management challenges faced by a TVET college and, by examining responses to these challenges, develop a sense of what leadership means in the sector. The study drew on three leadership theories – distributed leadership, transactional leadership as depicted in political models of management, and critical leadership – to help make sense of the findings. A qualitative case study design was used to explore key respondents’ views and lived experiences. The respondents were the principal, two deputy principals, a council member, three campus managers and a programme head. Interviews, questionnaires and document analysis were the chief data collection tools. The study found that critical leadership was the dominant approach at the college. This was revealed in the college leadership’s awareness of broader societal needs and its own role in operating in a socially just manner. College leadership also revealed signs of rejecting the status quo and opposing state control and bureaucracy, in favour of reactionary initiatives. There was limited evidence of distributed and transactional leadership. In fact, ‘leadership’ as such, seemed not to be part of the college discourse, suggesting that the concept and habit of leadership was not broadly discussed, shared and promoted. This sense was strengthened by the fact that at the time of the study, the college was headed by a charismatic and visionary leader. Indeed, the problem at the college seemed to be the Department of Higher Education and Training, which has failed the college in a number of ways.
- Full Text:
- Date Issued: 2020
- Authors: Chagi, Nonkonzo
- Date: 2020
- Subjects: Technical education -- South Africa , Vocational education -- South Africa , Educational leadership -- South Africa , Postsecondary education -- South Africa -- Administration
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/140045 , vital:37827
- Description: Far-reaching reforms of the TVET college system – including a merger and frequent revisioning of the colleges’ role in the educational landscape of South Africa – have focused renewed attention on this sector. The fact that the sector has been plagued by poor performance – even to the extent that several colleges have been placed under administration – suggests problems at the level of leadership. This study sought to explore key role-players’ understanding of the leadership and management challenges faced by a TVET college and, by examining responses to these challenges, develop a sense of what leadership means in the sector. The study drew on three leadership theories – distributed leadership, transactional leadership as depicted in political models of management, and critical leadership – to help make sense of the findings. A qualitative case study design was used to explore key respondents’ views and lived experiences. The respondents were the principal, two deputy principals, a council member, three campus managers and a programme head. Interviews, questionnaires and document analysis were the chief data collection tools. The study found that critical leadership was the dominant approach at the college. This was revealed in the college leadership’s awareness of broader societal needs and its own role in operating in a socially just manner. College leadership also revealed signs of rejecting the status quo and opposing state control and bureaucracy, in favour of reactionary initiatives. There was limited evidence of distributed and transactional leadership. In fact, ‘leadership’ as such, seemed not to be part of the college discourse, suggesting that the concept and habit of leadership was not broadly discussed, shared and promoted. This sense was strengthened by the fact that at the time of the study, the college was headed by a charismatic and visionary leader. Indeed, the problem at the college seemed to be the Department of Higher Education and Training, which has failed the college in a number of ways.
- Full Text:
- Date Issued: 2020
A critique of the language of record in South African courts in relation to selected university language policies
- Authors: Docrat, Zakeera
- Date: 2020
- Subjects: Language policy -- South Africa , Forensic linguistics -- South Africa , Communication in law -- South Africa , Language planning -- South Africa , Linguistic rights -- South Africa , Court interpreting and translating -- South Africa , Multilingual education -- South Africa , Language and education -- South Africa , Education, Higher -- Social aspects -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10962/141394 , vital:37968
- Description: This interdisciplinary research located in the research area of forensic linguistics seeks to provide a critique of the monolingual language of record directive for courts in South Africa, while investigating how university language policies contribute the formulation of a monolingual language of record policy for courts, by graduating monolingual LLB students. The research commences with identifying the research problem, goals and objectives and how the language of record policy for courts is linked to university language planning. The research proceeds to an overview of scholarly literature concerning the historical developments of language planning in both the legal system and higher education in South Africa. The theoretical principles concerning the enacting of language legislation and policies is advanced in relation to the constitutional framework. This research furthermore provides a thorough critique of the constitutional framework where the language rights the other related language provisions are discussed in relation to the theory and the application thereof in case law. The research explicates that the language rights of African language speaking litigants is unfairly limited and that access to justice for these litigants is either unattainable or achieved to a lesser extent. The disparities between language, law and power are brought to the fore, where the relevant legislation and language policies fail to determine the language of record in courts as well as legislate the African language requirements for legal practitioners in giving meaning to the constitutional language rights. The language policies of six selected universities is discussed in relation to the legal system’s legislative and policy frameworks, outlining the need to transform the language of learning and teaching and develop the curriculum to support the legal system. In doing so, the shortcomings of the interpretation profession in South Africa are highlighted and the effects thereof on the language rights of litigants. This thesis advances seven African and international case studies, comprising Kenya, Morocco, Nigeria, Australia, Belgium, Canada and India. Each of the case studies provides an in-depth analysis of the language of record/proceedings in courts and the language competencies of legal practitioners and judicial officers in relation to their university education. The African case studies are illustrative that English on the African continent in courts and higher education is dominate and the resultant loss of indigenous languages marginalises people from mainstream society. The international case studies provide two models, Belgium and Canada, which South Africa can emulate, in enacting new legislation and policies and the amendment of current legislation, to ensure bilingual/ multilingual language policies are drafted for courts per province, where the language demographics present majority spoken languages alongside English. Furthermore, where courts interpret language rights and legislative and policy provisions in a purposive manner, where African language speakers are able to, fully realise their rights. Australia and India as multilingual models serve as important case studies where South Africa can learn from what to avoid, how to subvert challenges or adequately address these. These case studies highlight the dangers of a political elite who pursue an English only agenda at the expense of the indigenous languages and the speakers thereof. This thesis in conclusion provides interdisciplinary recommendations that need to be implemented in order to address the language question in South African courts and higher education.
- Full Text:
- Date Issued: 2020
- Authors: Docrat, Zakeera
- Date: 2020
- Subjects: Language policy -- South Africa , Forensic linguistics -- South Africa , Communication in law -- South Africa , Language planning -- South Africa , Linguistic rights -- South Africa , Court interpreting and translating -- South Africa , Multilingual education -- South Africa , Language and education -- South Africa , Education, Higher -- Social aspects -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PHD
- Identifier: http://hdl.handle.net/10962/141394 , vital:37968
- Description: This interdisciplinary research located in the research area of forensic linguistics seeks to provide a critique of the monolingual language of record directive for courts in South Africa, while investigating how university language policies contribute the formulation of a monolingual language of record policy for courts, by graduating monolingual LLB students. The research commences with identifying the research problem, goals and objectives and how the language of record policy for courts is linked to university language planning. The research proceeds to an overview of scholarly literature concerning the historical developments of language planning in both the legal system and higher education in South Africa. The theoretical principles concerning the enacting of language legislation and policies is advanced in relation to the constitutional framework. This research furthermore provides a thorough critique of the constitutional framework where the language rights the other related language provisions are discussed in relation to the theory and the application thereof in case law. The research explicates that the language rights of African language speaking litigants is unfairly limited and that access to justice for these litigants is either unattainable or achieved to a lesser extent. The disparities between language, law and power are brought to the fore, where the relevant legislation and language policies fail to determine the language of record in courts as well as legislate the African language requirements for legal practitioners in giving meaning to the constitutional language rights. The language policies of six selected universities is discussed in relation to the legal system’s legislative and policy frameworks, outlining the need to transform the language of learning and teaching and develop the curriculum to support the legal system. In doing so, the shortcomings of the interpretation profession in South Africa are highlighted and the effects thereof on the language rights of litigants. This thesis advances seven African and international case studies, comprising Kenya, Morocco, Nigeria, Australia, Belgium, Canada and India. Each of the case studies provides an in-depth analysis of the language of record/proceedings in courts and the language competencies of legal practitioners and judicial officers in relation to their university education. The African case studies are illustrative that English on the African continent in courts and higher education is dominate and the resultant loss of indigenous languages marginalises people from mainstream society. The international case studies provide two models, Belgium and Canada, which South Africa can emulate, in enacting new legislation and policies and the amendment of current legislation, to ensure bilingual/ multilingual language policies are drafted for courts per province, where the language demographics present majority spoken languages alongside English. Furthermore, where courts interpret language rights and legislative and policy provisions in a purposive manner, where African language speakers are able to, fully realise their rights. Australia and India as multilingual models serve as important case studies where South Africa can learn from what to avoid, how to subvert challenges or adequately address these. These case studies highlight the dangers of a political elite who pursue an English only agenda at the expense of the indigenous languages and the speakers thereof. This thesis in conclusion provides interdisciplinary recommendations that need to be implemented in order to address the language question in South African courts and higher education.
- Full Text:
- Date Issued: 2020
A critique of translated (isiXhosa to English) sworn statements by the South African Police Service
- Authors: Mvabaza, Aviwe
- Date: 2020
- Subjects: Forensic linguistics , Translators -- South Africa , Xhosa language -- Translating , Police administration -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/122869 , vital:35361
- Description: The thesis provides a critique of sworn statement translations in the South African Police Service. The study is a mixed method study, which is rooted in Translation and Forensic Linguistics. It focuses on translations of two languages, IsiXhosa and English, an activity that was observed by the researcher at the Ndevana Police Station under the Buffalo City Municipality near King William’s Town in the Eastern Cape Province. In addition to the data collection strategies, among others, the study also collected data through the use of questionnaires responded to by the Ndevana Location’s community members. Furthermore, it used a method of consented interviews with the police officers. The main aim of these interviews was to unearth the police officers’ views on whether they require Translators and Forensic Linguists to assist them. Moreover, the other data collection strategy used was the consented recordings of verbal statements made by complainants, facilitated by the capturing of translated written dockets after the reporting of an incident. The police officers involved and who were available in the precinct were Constables, Sergeants, Detectives, Warrant officers and the Captain. The study period was 90 days, and the data presented in the form of graphs is contained in the relevant upcoming chapters. The findings of the study were that there was indeed a need for Translators and/or Forensic Linguists in the precinct, as the statistics revealed, by both the police officers and the community members. An analysis of the transcribed material, the interviews with the police and the verbal statements made by the complainants written by the police were evidence of the need for Translators and Forensic Linguists by the South African Police Services.
- Full Text:
- Date Issued: 2020
- Authors: Mvabaza, Aviwe
- Date: 2020
- Subjects: Forensic linguistics , Translators -- South Africa , Xhosa language -- Translating , Police administration -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/122869 , vital:35361
- Description: The thesis provides a critique of sworn statement translations in the South African Police Service. The study is a mixed method study, which is rooted in Translation and Forensic Linguistics. It focuses on translations of two languages, IsiXhosa and English, an activity that was observed by the researcher at the Ndevana Police Station under the Buffalo City Municipality near King William’s Town in the Eastern Cape Province. In addition to the data collection strategies, among others, the study also collected data through the use of questionnaires responded to by the Ndevana Location’s community members. Furthermore, it used a method of consented interviews with the police officers. The main aim of these interviews was to unearth the police officers’ views on whether they require Translators and Forensic Linguists to assist them. Moreover, the other data collection strategy used was the consented recordings of verbal statements made by complainants, facilitated by the capturing of translated written dockets after the reporting of an incident. The police officers involved and who were available in the precinct were Constables, Sergeants, Detectives, Warrant officers and the Captain. The study period was 90 days, and the data presented in the form of graphs is contained in the relevant upcoming chapters. The findings of the study were that there was indeed a need for Translators and/or Forensic Linguists in the precinct, as the statistics revealed, by both the police officers and the community members. An analysis of the transcribed material, the interviews with the police and the verbal statements made by the complainants written by the police were evidence of the need for Translators and Forensic Linguists by the South African Police Services.
- Full Text:
- Date Issued: 2020
A five year comparative analysis of annual baseline neurocognitive test scores for South African high school athletes
- Reichling, Marcelle Antoinette
- Authors: Reichling, Marcelle Antoinette
- Date: 2020
- Subjects: High school athletes -- Intelligence testing , Sports -- Psychological aspects , Neuropsychological tests , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Brain -- Concussion , Head -- Wounds and injuries
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/145863 , vital:38473
- Description: The primary objective of this study was to assess the pattern of change in neurocognitive performance for adolescent athletes on baseline measures of the Immediate Post Concussion Assessment and Cognitive Testing (ImPACT) test, over five consecutive years, with a view to providing an indication of the optimal interval for repeat baseline testing of high school athletes. Participants were non-clinical, predominantly South African high school athletes in the overall age range 13 to 18 years (N = 108), divided into five groups (Grades 8,9, 10, 11 and 12), and tested at five test intervals. Repeated-measures ANOVA analyses examined differences in score performance across the test intervals for each of the five composite scores of the ImPACT test (Verbal Memory, Visual Memory, Visual Motor Speed, Reaction Time, Impulse Control). For the Verbal Memory, Visual Memory, Visual Motor Speed and Reaction Time composites there were significant neurocognitive score changes between several test intervals. Taking these results into account, in conjunction with substantial variability in performance, it is concluded that there is a need for annual baseline testing throughout the high school years. The secondary objective was to generate normative tables (Means and Standard Deviations) on the ImPACT test for the five participant groups at each of the five test intervals, including data for: the five composite scores (Verbal Memory, Visual Memory, Visual Motor Speed, Reaction Time, Impulse Control); for the twelve subtest scores test that go to make up the composite scores; and for four additional memory subcomponent scores (Word Memory immediate recall, Word Memory delayed recall, Design Memory immediate recall, Design Memory delayed recall). The results provide a clinical and heuristic normative platform for future use with brain injured individuals, which can be used to facilitate clinical interpretations of postconcussion assessments.
- Full Text:
- Date Issued: 2020
- Authors: Reichling, Marcelle Antoinette
- Date: 2020
- Subjects: High school athletes -- Intelligence testing , Sports -- Psychological aspects , Neuropsychological tests , Brain -- Wounds and injuries -- Psychology , Sports injuries -- Psychological aspects , Brain -- Concussion , Head -- Wounds and injuries
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/145863 , vital:38473
- Description: The primary objective of this study was to assess the pattern of change in neurocognitive performance for adolescent athletes on baseline measures of the Immediate Post Concussion Assessment and Cognitive Testing (ImPACT) test, over five consecutive years, with a view to providing an indication of the optimal interval for repeat baseline testing of high school athletes. Participants were non-clinical, predominantly South African high school athletes in the overall age range 13 to 18 years (N = 108), divided into five groups (Grades 8,9, 10, 11 and 12), and tested at five test intervals. Repeated-measures ANOVA analyses examined differences in score performance across the test intervals for each of the five composite scores of the ImPACT test (Verbal Memory, Visual Memory, Visual Motor Speed, Reaction Time, Impulse Control). For the Verbal Memory, Visual Memory, Visual Motor Speed and Reaction Time composites there were significant neurocognitive score changes between several test intervals. Taking these results into account, in conjunction with substantial variability in performance, it is concluded that there is a need for annual baseline testing throughout the high school years. The secondary objective was to generate normative tables (Means and Standard Deviations) on the ImPACT test for the five participant groups at each of the five test intervals, including data for: the five composite scores (Verbal Memory, Visual Memory, Visual Motor Speed, Reaction Time, Impulse Control); for the twelve subtest scores test that go to make up the composite scores; and for four additional memory subcomponent scores (Word Memory immediate recall, Word Memory delayed recall, Design Memory immediate recall, Design Memory delayed recall). The results provide a clinical and heuristic normative platform for future use with brain injured individuals, which can be used to facilitate clinical interpretations of postconcussion assessments.
- Full Text:
- Date Issued: 2020
A formative intervention study of how learner voice and leadership can be developed within a Learner Representative Council (LRC) in an urban combined school, Namibia
- Authors: Shipopyeni, Salomo S M
- Date: 2020
- Subjects: Education, Secondary -- Namibia , Student government -- Namibia -- Case studies , Student participation in administration -- Namibia -- Case studies
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/144934 , vital:38393
- Description: The Namibian Education system, after the country gained independence, introduced various reforms to ensure the attainment of the educational goals of access, quality, equity and democracy in schools. One of the policies introduced to promote democracy in our schools was The Educational Act 16 of 2001, which gave birth to the establishment of Learners Representatives Councils (LRC) in schools. The LRC body is the legal learner leadership body established to ensure learners are represented in school leadership. However, various studies have revealed that this legal body of learners in many schools has been merely ‘rubber-stamping’ decisions made by teachers; learners have had very little input in decisions that affect them as learners. Thus, I was prompted to conduct this formative intervention study on learner leadership at an urban combined school in Namibia. Informed by distributed and transformative leadership theories, the study aimed to develop leadership within the LRC members and the needed expansive transformation regarding leadership practices in school. The intention was for learners to be enabled to practice their democratic right in decision-making processes in matters that concerned their schooling and learning. This study was theoretically and analytically framed by second generation CulturalHistorical Activity Theory. The participants included 12 LRC members, the LRC liaison teacher, the class register teacher, three school management team members and the principal. The research method was a case study, underpinned by the critical paradigm to bring about the fundamental expansive transformation in learner leadership practices at the case study school. This qualitative study was divided into two phases, a contextual profiling phase and an intervention phase. Data were generated through document analysis, observation, questionnaires, focus group interviews and Change Laboratory Workshops. The data were generated to answer the over-arching question: How learner voice and leadership can be developed within a Learner Representative Council (LRC) in an urban combined school, Namibia. The data were analysed inductively and abductively. The key findings were: first, there were a variety of understandings of the concept learner leadership; second, the involvement of LRC members in decision-making processes was limited to involvement in organising extracurricular activities and controlling of other learners at school; third, leadership development opportunities for learners at the case study school were only provided through training at the beginning of the year and the LRC carrying out various activities and roles at the case study school. Several challenges that constrained the LRC voice and leadership development were surfaced and, through Change Laboratory Workshops, the participants of the activity system together with me (the researcher-interventionist), identified the expansive learning opportunities to develop leadership amongst Learner Representative Council (LRC) members. In the final analysis, this study will contribute to the production of knowledge on the concept of learner leadership in the context of Namibia. Fellow scholars, professionals, colleagues and policy makers in education are requested to engage with this thesis to contribute to our understanding of this important aspect of our field and speak back to policy.
- Full Text:
- Date Issued: 2020
- Authors: Shipopyeni, Salomo S M
- Date: 2020
- Subjects: Education, Secondary -- Namibia , Student government -- Namibia -- Case studies , Student participation in administration -- Namibia -- Case studies
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/144934 , vital:38393
- Description: The Namibian Education system, after the country gained independence, introduced various reforms to ensure the attainment of the educational goals of access, quality, equity and democracy in schools. One of the policies introduced to promote democracy in our schools was The Educational Act 16 of 2001, which gave birth to the establishment of Learners Representatives Councils (LRC) in schools. The LRC body is the legal learner leadership body established to ensure learners are represented in school leadership. However, various studies have revealed that this legal body of learners in many schools has been merely ‘rubber-stamping’ decisions made by teachers; learners have had very little input in decisions that affect them as learners. Thus, I was prompted to conduct this formative intervention study on learner leadership at an urban combined school in Namibia. Informed by distributed and transformative leadership theories, the study aimed to develop leadership within the LRC members and the needed expansive transformation regarding leadership practices in school. The intention was for learners to be enabled to practice their democratic right in decision-making processes in matters that concerned their schooling and learning. This study was theoretically and analytically framed by second generation CulturalHistorical Activity Theory. The participants included 12 LRC members, the LRC liaison teacher, the class register teacher, three school management team members and the principal. The research method was a case study, underpinned by the critical paradigm to bring about the fundamental expansive transformation in learner leadership practices at the case study school. This qualitative study was divided into two phases, a contextual profiling phase and an intervention phase. Data were generated through document analysis, observation, questionnaires, focus group interviews and Change Laboratory Workshops. The data were generated to answer the over-arching question: How learner voice and leadership can be developed within a Learner Representative Council (LRC) in an urban combined school, Namibia. The data were analysed inductively and abductively. The key findings were: first, there were a variety of understandings of the concept learner leadership; second, the involvement of LRC members in decision-making processes was limited to involvement in organising extracurricular activities and controlling of other learners at school; third, leadership development opportunities for learners at the case study school were only provided through training at the beginning of the year and the LRC carrying out various activities and roles at the case study school. Several challenges that constrained the LRC voice and leadership development were surfaced and, through Change Laboratory Workshops, the participants of the activity system together with me (the researcher-interventionist), identified the expansive learning opportunities to develop leadership amongst Learner Representative Council (LRC) members. In the final analysis, this study will contribute to the production of knowledge on the concept of learner leadership in the context of Namibia. Fellow scholars, professionals, colleagues and policy makers in education are requested to engage with this thesis to contribute to our understanding of this important aspect of our field and speak back to policy.
- Full Text:
- Date Issued: 2020
A gendered analysis of conditional cash based transfers: a case study of Puntland Technical Vocational Skills Training Programme, Somalia
- Authors: Chitombi, Rumbidzai
- Date: 2020
- Subjects: Puntland Technical Vocational Skills Training Programme , Transfer payments -- Somalia -- Case studies , Economic assistance, Domestic -- Somalia , Economic development -- Social aspects -- Somalia , Women -- Somalia -- Social conditions
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167335 , vital:41469
- Description: As part of the worldwide development system, conditional cash transfer (CCT) programmes have become an increasingly popular policy and development approach in seeking to address poverty, especially in developing countries. Under the CCT programmes, beneficiaries are given assistance in the form of either cash or cash vouchers after fulfilling certain obligations of the development programme, such as attending training, enacting proper health care, or ensuring regular school attendance of children. The programmes have been described as a ‘double-edged sword’ since they aim to address poverty and, at the same time, reduce reliance on government largesse. In this regard, they are seen as potentially effective, and more empowering, alternatives to more traditional social assistance programmes whereby poor people receive welfare assistance in the form of ‘in kind’ and ‘unconditional’ assistance, receiving this as either food or shelter commodities, and without having to meet any conditions in doing so. This ‘traditional’ way of assisting poor people has largely been criticised for creating a dependency syndrome amongst the beneficiaries. In certain cases, CCT programmes focus specifically on women, either in receiving the cash transfer or in meeting the conditions attached to the programme, or both. In this context, considerable debate exists in the scholarly literature about the effects of such CCT programmes on the situation and status of women, specifically in terms of possibly empowering women. While some scholars claim that these programmes enhance the human and financial assets of women, others argue that focusing specifically on women, and as care-givers within households, tends to reproduce gender-based inequalities and subordination. Since gender equality and female empowerment are now key issues in global development spheres, and at national levels, this thesis aims to contribute to literature on the effects of CCTs on gender and women’s empowerment. This is pursued by way of a gendered perspective on CCTs as a development methodology for empowering women with reference to Somalia, using the Puntland Technical Vocational Skills Training programme as a case study. This programme focused, in the main, on internally-displaced people in Somalia, with a particular emphasis on women in meeting the programme conditions (i.e. participating in a training programme) and in being the cash recipients. The study used both quantitative and qualitative approaches for data collection and analysis, focusing on sixty selected beneficiaries who participated in the Puntland Technical Vocational skills training programme in Somalia from 2013. The thesis examines the prevailing structures (including cultural dynamics and socio-economic factors) in Somalia which lead to women’s subordination, notably in the light of significant internal displacement because of war and conflict and the emergence of internally-displaced camps. On this basis, from a gendered perspective, there is a critical appraisal of the manner in which the Puntland CCT programme affected women’s subordinate status, including how it may have led to the restructuring of gendered relations at both household and community levels. In offering this appraisal with reference to the Puntland programme, the thesis argues that women’s subordination and, by extension, women’s empowerment, is multi-faceted, and that continuity and change along the dimensions of subordination is often uneven and contradictory. Further, as also demonstrated in the Puntland case study, women’s subordination (as a social totality) is not a totalising system, such that women regularly make use of gaps in the system as opportunities to enhance their well-being without confronting the totality of the system.
- Full Text:
- Date Issued: 2020
- Authors: Chitombi, Rumbidzai
- Date: 2020
- Subjects: Puntland Technical Vocational Skills Training Programme , Transfer payments -- Somalia -- Case studies , Economic assistance, Domestic -- Somalia , Economic development -- Social aspects -- Somalia , Women -- Somalia -- Social conditions
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167335 , vital:41469
- Description: As part of the worldwide development system, conditional cash transfer (CCT) programmes have become an increasingly popular policy and development approach in seeking to address poverty, especially in developing countries. Under the CCT programmes, beneficiaries are given assistance in the form of either cash or cash vouchers after fulfilling certain obligations of the development programme, such as attending training, enacting proper health care, or ensuring regular school attendance of children. The programmes have been described as a ‘double-edged sword’ since they aim to address poverty and, at the same time, reduce reliance on government largesse. In this regard, they are seen as potentially effective, and more empowering, alternatives to more traditional social assistance programmes whereby poor people receive welfare assistance in the form of ‘in kind’ and ‘unconditional’ assistance, receiving this as either food or shelter commodities, and without having to meet any conditions in doing so. This ‘traditional’ way of assisting poor people has largely been criticised for creating a dependency syndrome amongst the beneficiaries. In certain cases, CCT programmes focus specifically on women, either in receiving the cash transfer or in meeting the conditions attached to the programme, or both. In this context, considerable debate exists in the scholarly literature about the effects of such CCT programmes on the situation and status of women, specifically in terms of possibly empowering women. While some scholars claim that these programmes enhance the human and financial assets of women, others argue that focusing specifically on women, and as care-givers within households, tends to reproduce gender-based inequalities and subordination. Since gender equality and female empowerment are now key issues in global development spheres, and at national levels, this thesis aims to contribute to literature on the effects of CCTs on gender and women’s empowerment. This is pursued by way of a gendered perspective on CCTs as a development methodology for empowering women with reference to Somalia, using the Puntland Technical Vocational Skills Training programme as a case study. This programme focused, in the main, on internally-displaced people in Somalia, with a particular emphasis on women in meeting the programme conditions (i.e. participating in a training programme) and in being the cash recipients. The study used both quantitative and qualitative approaches for data collection and analysis, focusing on sixty selected beneficiaries who participated in the Puntland Technical Vocational skills training programme in Somalia from 2013. The thesis examines the prevailing structures (including cultural dynamics and socio-economic factors) in Somalia which lead to women’s subordination, notably in the light of significant internal displacement because of war and conflict and the emergence of internally-displaced camps. On this basis, from a gendered perspective, there is a critical appraisal of the manner in which the Puntland CCT programme affected women’s subordinate status, including how it may have led to the restructuring of gendered relations at both household and community levels. In offering this appraisal with reference to the Puntland programme, the thesis argues that women’s subordination and, by extension, women’s empowerment, is multi-faceted, and that continuity and change along the dimensions of subordination is often uneven and contradictory. Further, as also demonstrated in the Puntland case study, women’s subordination (as a social totality) is not a totalising system, such that women regularly make use of gaps in the system as opportunities to enhance their well-being without confronting the totality of the system.
- Full Text:
- Date Issued: 2020
A genetic analysis of the species and intraspecific lineages of Dactylopius Costa (Hemiptera: Dactylopiidae)
- Van Steenderen, Clarke Julian Mignon
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2020
- Subjects: Dactylopius
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/151491 , vital:39135
- Description: The Cactaceae family comprises 15 genera and nearly 2000 species. With one exception, these are all native to the Americas. Numerous cactaceous species are invasive in other parts of the world, resulting in considerable damage to ecosystem functioning and agricultural practices. The most successful biological control agents used to combat invasive Cactaceae belong to the Dactylopius genus (Hemiptera: Dactylopiidae), comprising eleven species. The Dactylopiidae are exclusively cactophagous and are usually host-specific. Some intraspecific lineages of dactylopiids, often referred to as `biotypes', also display host-specificity, and are used to control particular species of invasive Cactaceae. To date, two lineages within Dactylopius opuntiae (`ficus' and `stricta'), and two within D. tomentosus (`cholla' and `imbricata') have been released in South Africa to control Opuntia ficus-indica and O. stricta, and Cylindropuntia fulgida and C. imbricata, respectively. The `californica var. parkeri' lineage is currently under consideration for release in South Africa for the control of C. pallida. Australia has already released these five lineages, and approved the release of an additional three in 2017; namely D. tomentosus `bigelovii', `cylindropuntia sp.', and `acanthocarpa x echinocarpa'. Many of the Dactylopius species are so morphologically similar, and in the case of lineages, identical, that numerous misidentifications have been made in the past. These errors have had serious implications, such as failed attempts at the biological control of cactus weeds. This thesis aimed to generate a multi-locus genetic database to enable the identification of the species and lineages in the Dactylopiidae family, and to test its accuracy. Seven species were included in the analysis, including two lineages within D. opuntiae and six within D. tomentosus. Genetic characterisation was achieved through the DNA sequencing of three gene regions; namely mitochondrial 12S rRNA and cytochrome c oxidase I (COI), nuclear 18S rRNA, and fragment analysis using two inter-simple sequence repeats (ISSRs). Nucleotide sequences were very effective for species-level identification, where the 12S, 18S, and COI regions showed 100%, 94.59%, and 100% identification accuracy rates, respectively. Additionally, the 12S and COI markers distinguished between half of the D. tomentosus lineages (`californica', `cholla', and `imbricata'), with identification accuracies of 100%. The `echinocarpa x acanthocarpa', `bigelovii', and `cylindropuntia sp.' lineages formed one clade. None of the DNA genetic markers showed a separation between the `ficus' and `stricta' lineages within D. opuntiae. Fragment analysis through the use of ISSRs provided higher-resolution results, and addressed this gap by showing a well-supported separation between the two lineages, and between wild populations collected in the Eastern Cape Province in South Africa. The identification accuracy of the `ficus' and `stricta' lineages was 81.82%. This is the first time that a method has been developed that can distinguish between these lineages. An additional component of this thesis was the creation of three user-friendly R-based programs to assist with: 1. ISSR data processing. 2. The identification of query Dactylopius nucleotide sequences relative to the gene databases created here. 3. A graphical user interface (GUI) version of the R package `SPIDER', which is useful for the assessment of the accuracy of genetic barcode data. A successful biological control programme relies on the correct identification of the agent in question, and so it is imperative that cactus biological control practitioners are able to distinguish between Dactylopius species and lineages in order to release the most effective ones onto target Cactaceae. The laboratory protocols reported, and data processing tools created here, have largely addressed this need and offer valuable practical applications. These include: 1. The flagging of potential new species, cryptic species, and lineages of dactylopiid species released as new biocontrol agents. 2. Validating the identifications made by taxonomists based on morphology. 3. Confirming to which species, and, where applicable, to which lineage, a field-collected sample belongs. 4. Identifying hybrids resulting from lineage crosses. Ensuring that the correct Dactylopius species are utilised for biological control will improve the control of invasive Cactaceae and protect biodiversity and agricultural productivity.
- Full Text:
- Date Issued: 2020
- Authors: Van Steenderen, Clarke Julian Mignon
- Date: 2020
- Subjects: Dactylopius
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/151491 , vital:39135
- Description: The Cactaceae family comprises 15 genera and nearly 2000 species. With one exception, these are all native to the Americas. Numerous cactaceous species are invasive in other parts of the world, resulting in considerable damage to ecosystem functioning and agricultural practices. The most successful biological control agents used to combat invasive Cactaceae belong to the Dactylopius genus (Hemiptera: Dactylopiidae), comprising eleven species. The Dactylopiidae are exclusively cactophagous and are usually host-specific. Some intraspecific lineages of dactylopiids, often referred to as `biotypes', also display host-specificity, and are used to control particular species of invasive Cactaceae. To date, two lineages within Dactylopius opuntiae (`ficus' and `stricta'), and two within D. tomentosus (`cholla' and `imbricata') have been released in South Africa to control Opuntia ficus-indica and O. stricta, and Cylindropuntia fulgida and C. imbricata, respectively. The `californica var. parkeri' lineage is currently under consideration for release in South Africa for the control of C. pallida. Australia has already released these five lineages, and approved the release of an additional three in 2017; namely D. tomentosus `bigelovii', `cylindropuntia sp.', and `acanthocarpa x echinocarpa'. Many of the Dactylopius species are so morphologically similar, and in the case of lineages, identical, that numerous misidentifications have been made in the past. These errors have had serious implications, such as failed attempts at the biological control of cactus weeds. This thesis aimed to generate a multi-locus genetic database to enable the identification of the species and lineages in the Dactylopiidae family, and to test its accuracy. Seven species were included in the analysis, including two lineages within D. opuntiae and six within D. tomentosus. Genetic characterisation was achieved through the DNA sequencing of three gene regions; namely mitochondrial 12S rRNA and cytochrome c oxidase I (COI), nuclear 18S rRNA, and fragment analysis using two inter-simple sequence repeats (ISSRs). Nucleotide sequences were very effective for species-level identification, where the 12S, 18S, and COI regions showed 100%, 94.59%, and 100% identification accuracy rates, respectively. Additionally, the 12S and COI markers distinguished between half of the D. tomentosus lineages (`californica', `cholla', and `imbricata'), with identification accuracies of 100%. The `echinocarpa x acanthocarpa', `bigelovii', and `cylindropuntia sp.' lineages formed one clade. None of the DNA genetic markers showed a separation between the `ficus' and `stricta' lineages within D. opuntiae. Fragment analysis through the use of ISSRs provided higher-resolution results, and addressed this gap by showing a well-supported separation between the two lineages, and between wild populations collected in the Eastern Cape Province in South Africa. The identification accuracy of the `ficus' and `stricta' lineages was 81.82%. This is the first time that a method has been developed that can distinguish between these lineages. An additional component of this thesis was the creation of three user-friendly R-based programs to assist with: 1. ISSR data processing. 2. The identification of query Dactylopius nucleotide sequences relative to the gene databases created here. 3. A graphical user interface (GUI) version of the R package `SPIDER', which is useful for the assessment of the accuracy of genetic barcode data. A successful biological control programme relies on the correct identification of the agent in question, and so it is imperative that cactus biological control practitioners are able to distinguish between Dactylopius species and lineages in order to release the most effective ones onto target Cactaceae. The laboratory protocols reported, and data processing tools created here, have largely addressed this need and offer valuable practical applications. These include: 1. The flagging of potential new species, cryptic species, and lineages of dactylopiid species released as new biocontrol agents. 2. Validating the identifications made by taxonomists based on morphology. 3. Confirming to which species, and, where applicable, to which lineage, a field-collected sample belongs. 4. Identifying hybrids resulting from lineage crosses. Ensuring that the correct Dactylopius species are utilised for biological control will improve the control of invasive Cactaceae and protect biodiversity and agricultural productivity.
- Full Text:
- Date Issued: 2020
A holistic approach in understanding the effects of dietary protein sources on the growth and reproductive development of farmed abalone, Haliotis midae
- Wu, Yu
- Authors: Wu, Yu
- Date: 2020
- Subjects: Abalones -- Nutrition , Abalones -- Reproduction , Abalones -- Growth , Abalone culture , Haliotis midae -- Nutrition , Haliotis midae -- Reproduction , Haliotis midae -- Growth , Haliotis midae fisheries
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167719 , vital:41506
- Description: The combination of fishmeal and soya in the formulated feed of farmed South African abalone, Haliotis midae, not only improved abalone growth, but also the sustainability of the compound diets by reducing the reliance on fishmeal. However, the presence of soya produced larger gonads compared to those of abalone fed kelp or single-source protein diets. There is an increasing drive to control sexual maturation and reduce undesirable spawning events in farmed abalone. However, the reasons for the reported effects of soya inclusion on the reproductive development of farmed H. midae remain unresolved. The aim of this research was to use a combination of techniques to contribute to the understanding of the relationship between growth, reproductive and nutritional physiology of farmed H. midae fed diets of varying protein sources. These techniques included stable isotope analysis (SIA), fatty acid analysis, gonad histological assessments and haemolymph sexsteroid hormone analysis. The objectives were to examine the allocation of protein and nutritional components to somatic and reproductive tissues. This study also included the first attempt to investigate the role that sex steroid hormones play during gametogenesis. Abalone (40 – 50 g abalone-1) were fed one of four diets: either a single-source protein-based diet, i.e., (1) fishmeal-only (FM) or (2) soya-only (S), or a combination diet of (3) fishmeal-soya (FM S) or (4) fishmeal-sunflower meal (FM SM). Sampling occurred in 45-day intervals over one year. Dietary protein source had an effect on the growth of abalone, with enhancements in growth linked to the combination of fishmeal and a plant-protein source. The fishmeal ingredient was the most utilised protein source throughout the temporal changes in protein allocation into somatic and reproductive tissue, followed by soya and then sunflower meal. The mean whole- body mass of abalone was significantly influenced by an interaction between time and dietary treatment, with average whole-body mass changing differently over time between treatments (RM-ANOVA, F(24, 96) = 2.13, p = 0.005). Overall, abalone that were fed FM S had higher mean whole-body mass values than abalone that were fed the single-protein based diets, while abalone that were fed FM SM were similar to animals from all dietary treatments (RMANOVA, F(3, 12) = 5.75, p = 0.01). Male abalone had significantly higher gonad bulk index (GBI) values compared to females (RM-ANOVA, F(1, 24) = 49.03, p < 0.001) and this was independent of dietary treatment. Within each sex, female abalone fed the FM S diet (15.92 ± 1.88 mm3 g-1) had significantly higher GBI values than abalone fed S (9.76 ± 1.08 mm3 g-1), while abalone fed FM (11.96 ± 1.71 mm3 g-1) and FM SM (11.90 ± 0.80 mm3 g-1) were similar to abalone from all other dietary treatments (Tukey’s HSD, p < 0.05). Male abalone fed the FM S (21.59 ± 2.10 mm3 g-1) and FM SM diet (19.30 ± 2.63 mm3 g-1) had similar GBI values and they were significantly higher than in abalone fed the S diet (14.74 ± 1.27 mm3 g-1), while abalone fed FM S had significantly higher GBI values than abalone that consumed the FM diet (15.08 ±1.63mm3 g-1) ( Tukey’s HSD, p < 0.05). Although sunflower meal was poorly utilised, it produced similar overall growth to abalone that were fed the FM S diets. Yet, feed conversion ratio values were significantly lower for abalone fed FM S (1.30 ± 0.13) compared to those in the other three treatments, with ratios ranging from 1.65 – 1.72 over the one-year. The fatty acid compositions of the somatic and gonadal tissues were similar between treatments (PERMANOVA, p = 0.21), while fatty acid composition was influenced by sampling day, tissue type and abalone sex (PERMANOVA, p < 0.05). The essential fatty acids (EFAs) eicosadienoic acid and a-linolenic acid were present in abalone tissue, but they were not detected in the diets, suggesting the important role that an alternate food source (e.g. farmed abalone also had access to diatoms) may have played and the ability that H. midae may have in converting long-chain polyunsaturated fatty acids from C18 precursors. A change in the abundance of EFAs in the gonad tissue during highest and lowest GBI values suggested that arachidonic, eicosapentaenoic, g-linolenic and linoleic acid were important in females, while eicosapentaenoic, eicosadienoic and a-linolenic acid were important for male reproductive development. Dietary protein sources had an effect on the frequency distribution of maturity stages, where females that were fed FM S produced more ripe gonads and more males that were fed FM SM contained testes that showed signs of ripeness over the one-year study. Although dietary protein influenced the sex steroid concentrations in females and males, exhibiting fluctuations throughout the one-year period, no distinct pattern linked to gametogenesis were observed. The results from this study illustrate: (1) the importance of conducting laboratory studies when implementing SIA and mixing models in aquaculture nutrition; (2) conducting nutritional studies on mature, grow-out abalone; and (3) assessing the importance of naturally occurring diatoms in their diet and their contribution to growth and reproduction. The novel contribution of this research towards abalone nutritional physiology, the implications of these findings to industry as well as potential considerations for future studies were addressed.
- Full Text:
- Date Issued: 2020
- Authors: Wu, Yu
- Date: 2020
- Subjects: Abalones -- Nutrition , Abalones -- Reproduction , Abalones -- Growth , Abalone culture , Haliotis midae -- Nutrition , Haliotis midae -- Reproduction , Haliotis midae -- Growth , Haliotis midae fisheries
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/167719 , vital:41506
- Description: The combination of fishmeal and soya in the formulated feed of farmed South African abalone, Haliotis midae, not only improved abalone growth, but also the sustainability of the compound diets by reducing the reliance on fishmeal. However, the presence of soya produced larger gonads compared to those of abalone fed kelp or single-source protein diets. There is an increasing drive to control sexual maturation and reduce undesirable spawning events in farmed abalone. However, the reasons for the reported effects of soya inclusion on the reproductive development of farmed H. midae remain unresolved. The aim of this research was to use a combination of techniques to contribute to the understanding of the relationship between growth, reproductive and nutritional physiology of farmed H. midae fed diets of varying protein sources. These techniques included stable isotope analysis (SIA), fatty acid analysis, gonad histological assessments and haemolymph sexsteroid hormone analysis. The objectives were to examine the allocation of protein and nutritional components to somatic and reproductive tissues. This study also included the first attempt to investigate the role that sex steroid hormones play during gametogenesis. Abalone (40 – 50 g abalone-1) were fed one of four diets: either a single-source protein-based diet, i.e., (1) fishmeal-only (FM) or (2) soya-only (S), or a combination diet of (3) fishmeal-soya (FM S) or (4) fishmeal-sunflower meal (FM SM). Sampling occurred in 45-day intervals over one year. Dietary protein source had an effect on the growth of abalone, with enhancements in growth linked to the combination of fishmeal and a plant-protein source. The fishmeal ingredient was the most utilised protein source throughout the temporal changes in protein allocation into somatic and reproductive tissue, followed by soya and then sunflower meal. The mean whole- body mass of abalone was significantly influenced by an interaction between time and dietary treatment, with average whole-body mass changing differently over time between treatments (RM-ANOVA, F(24, 96) = 2.13, p = 0.005). Overall, abalone that were fed FM S had higher mean whole-body mass values than abalone that were fed the single-protein based diets, while abalone that were fed FM SM were similar to animals from all dietary treatments (RMANOVA, F(3, 12) = 5.75, p = 0.01). Male abalone had significantly higher gonad bulk index (GBI) values compared to females (RM-ANOVA, F(1, 24) = 49.03, p < 0.001) and this was independent of dietary treatment. Within each sex, female abalone fed the FM S diet (15.92 ± 1.88 mm3 g-1) had significantly higher GBI values than abalone fed S (9.76 ± 1.08 mm3 g-1), while abalone fed FM (11.96 ± 1.71 mm3 g-1) and FM SM (11.90 ± 0.80 mm3 g-1) were similar to abalone from all other dietary treatments (Tukey’s HSD, p < 0.05). Male abalone fed the FM S (21.59 ± 2.10 mm3 g-1) and FM SM diet (19.30 ± 2.63 mm3 g-1) had similar GBI values and they were significantly higher than in abalone fed the S diet (14.74 ± 1.27 mm3 g-1), while abalone fed FM S had significantly higher GBI values than abalone that consumed the FM diet (15.08 ±1.63mm3 g-1) ( Tukey’s HSD, p < 0.05). Although sunflower meal was poorly utilised, it produced similar overall growth to abalone that were fed the FM S diets. Yet, feed conversion ratio values were significantly lower for abalone fed FM S (1.30 ± 0.13) compared to those in the other three treatments, with ratios ranging from 1.65 – 1.72 over the one-year. The fatty acid compositions of the somatic and gonadal tissues were similar between treatments (PERMANOVA, p = 0.21), while fatty acid composition was influenced by sampling day, tissue type and abalone sex (PERMANOVA, p < 0.05). The essential fatty acids (EFAs) eicosadienoic acid and a-linolenic acid were present in abalone tissue, but they were not detected in the diets, suggesting the important role that an alternate food source (e.g. farmed abalone also had access to diatoms) may have played and the ability that H. midae may have in converting long-chain polyunsaturated fatty acids from C18 precursors. A change in the abundance of EFAs in the gonad tissue during highest and lowest GBI values suggested that arachidonic, eicosapentaenoic, g-linolenic and linoleic acid were important in females, while eicosapentaenoic, eicosadienoic and a-linolenic acid were important for male reproductive development. Dietary protein sources had an effect on the frequency distribution of maturity stages, where females that were fed FM S produced more ripe gonads and more males that were fed FM SM contained testes that showed signs of ripeness over the one-year study. Although dietary protein influenced the sex steroid concentrations in females and males, exhibiting fluctuations throughout the one-year period, no distinct pattern linked to gametogenesis were observed. The results from this study illustrate: (1) the importance of conducting laboratory studies when implementing SIA and mixing models in aquaculture nutrition; (2) conducting nutritional studies on mature, grow-out abalone; and (3) assessing the importance of naturally occurring diatoms in their diet and their contribution to growth and reproduction. The novel contribution of this research towards abalone nutritional physiology, the implications of these findings to industry as well as potential considerations for future studies were addressed.
- Full Text:
- Date Issued: 2020
A leopard's favourite spots: habitat preference and population density of leopards in a semi-arid biodiversity hotspot
- Mann, Gareth K H, O'Riain, M Justin, Parker, Daniel M
- Authors: Mann, Gareth K H , O'Riain, M Justin , Parker, Daniel M
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148584 , vital:38752 , https://doi.org/10.1016/j.jaridenv.2020.104218
- Description: Leopards (Panthera pardus) are the only free-ranging large predators to still occur naturally throughout much of Africa, but are vulnerable to habitat loss, ecosystem degradation and persecution. We used a systematic camera trap survey covering an area of ∼3100 km2 in the Little Karoo, a semi-arid biodiversity hotspot in South Africa, to assess the impacts of land use and habitat type on leopard density. Camera data were supplemented by opportunistic scat collection to produce a habitat suitability model. We used a habitat suitability model to inform spatially explicit capture-recapture models used to estimate population density. We recorded 152 independent photographs of 27 individually recognizable leopards at 54 camera stations and collected scats from a further 76 locations. Our habitat suitability model showed that primary productivity and vegetation type were the best predictors of leopard habitat suitability. Our best performing population density model allowed for detection and movement of individuals to vary according to sex, and estimated population density at 1.26 (SE ± 0.25) leopards/100 km2.
- Full Text:
- Date Issued: 2020
- Authors: Mann, Gareth K H , O'Riain, M Justin , Parker, Daniel M
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148584 , vital:38752 , https://doi.org/10.1016/j.jaridenv.2020.104218
- Description: Leopards (Panthera pardus) are the only free-ranging large predators to still occur naturally throughout much of Africa, but are vulnerable to habitat loss, ecosystem degradation and persecution. We used a systematic camera trap survey covering an area of ∼3100 km2 in the Little Karoo, a semi-arid biodiversity hotspot in South Africa, to assess the impacts of land use and habitat type on leopard density. Camera data were supplemented by opportunistic scat collection to produce a habitat suitability model. We used a habitat suitability model to inform spatially explicit capture-recapture models used to estimate population density. We recorded 152 independent photographs of 27 individually recognizable leopards at 54 camera stations and collected scats from a further 76 locations. Our habitat suitability model showed that primary productivity and vegetation type were the best predictors of leopard habitat suitability. Our best performing population density model allowed for detection and movement of individuals to vary according to sex, and estimated population density at 1.26 (SE ± 0.25) leopards/100 km2.
- Full Text:
- Date Issued: 2020
A mathematics teacher's response to a dilemma: 'I'm supposed to teach them in English but they don't understand'
- Robertson, Sally-Ann, Graven, Mellony
- Authors: Robertson, Sally-Ann , Graven, Mellony
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148889 , vital:38783 , https://doi.org/10.4102/sajce.v10i1.800
- Description: English is the dominant language in South African schools although it is the home language for less than 10% of the population. Many schools have yet to embrace the Language in Education Policy’s advocacy of additive bilingualism. This has led to a majority of the country’s children learning and being assessed through a language in which they lack proficiency. This article draws on second language teaching and learning theory to make a case for more systematic support for learners’ second language development and for legitimation of use of home language in mathematics classrooms where a different language is the official medium. The article shares empirical data from a South African Grade 4 mathematics teacher’s classroom to illuminate arguments in favour of additive bilingualism.
- Full Text:
- Date Issued: 2020
- Authors: Robertson, Sally-Ann , Graven, Mellony
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148889 , vital:38783 , https://doi.org/10.4102/sajce.v10i1.800
- Description: English is the dominant language in South African schools although it is the home language for less than 10% of the population. Many schools have yet to embrace the Language in Education Policy’s advocacy of additive bilingualism. This has led to a majority of the country’s children learning and being assessed through a language in which they lack proficiency. This article draws on second language teaching and learning theory to make a case for more systematic support for learners’ second language development and for legitimation of use of home language in mathematics classrooms where a different language is the official medium. The article shares empirical data from a South African Grade 4 mathematics teacher’s classroom to illuminate arguments in favour of additive bilingualism.
- Full Text:
- Date Issued: 2020
A mixed methods investigation of students’ attitudes towards statistics and quantitative research methods: a focus on postgraduate psychology students at a South African university
- Authors: Ngantweni, Xolelwa
- Date: 2020
- Subjects: Fetal alcohol spectrum disorders -- South Africa , Statistics , Psychology -- Research , College students -- South Africa -- Attitudes , Psychology -- Research -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/140490 , vital:37894
- Description: Many University programs offer a course in either basic or intermediate statistics as part of the degree requirements prior to graduation (McGrath, Ferns, Greiner, Wanamaker and Brown, 2015). These statistics or quantitative research methods courses are integral in helping students gain vital skills in analysing quantitative data. Research (Schau, Stevens, Dauphinee, and Del Vecchio, 1995) does however indicate that most students have a perfunctory disposition towards these courses. My study sought to particularly investigate attitudes towards statistics and quantitative research methods amongst a sample of 61 postgraduate Psychology students at Rhodes University undertaking a ‘Quantitative Research Methods’ course as part of their degree offering. A mixed methods approach was used to investigate students’ attitudes towards statistics and quantitative research methods. The Survey of Attitudes Toward Statistics (SATS-36) (Schau, 2003) captured student’s attitudes towards statistics using a Likert Scale instrument; whereas detailed qualitative interviews accentuated findings from the SATS-36. Key quantitative findings from the SATS-36 including students’ perceptions of statistics being a difficult course as well as students having a low affect towards statistics are detailed. Key qualitative findings related to why students experience statistics anxiety such as students’ (1) fear of failing statistics, (2) The late introduction of statistics in the Psychology curriculum, and (3) The role of educator/s in alleviating or promoting feelings of statistics anxiety are noted. The significance of these findings as well as the contributions of the study to the teaching and learning of statistics and quantitative research methods courses at Rhodes University are explored, in light of other studies on the topic of statistics anxiety and attitudes towards statistics/ quantitative research methods.
- Full Text:
- Date Issued: 2020
- Authors: Ngantweni, Xolelwa
- Date: 2020
- Subjects: Fetal alcohol spectrum disorders -- South Africa , Statistics , Psychology -- Research , College students -- South Africa -- Attitudes , Psychology -- Research -- South Africa
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/140490 , vital:37894
- Description: Many University programs offer a course in either basic or intermediate statistics as part of the degree requirements prior to graduation (McGrath, Ferns, Greiner, Wanamaker and Brown, 2015). These statistics or quantitative research methods courses are integral in helping students gain vital skills in analysing quantitative data. Research (Schau, Stevens, Dauphinee, and Del Vecchio, 1995) does however indicate that most students have a perfunctory disposition towards these courses. My study sought to particularly investigate attitudes towards statistics and quantitative research methods amongst a sample of 61 postgraduate Psychology students at Rhodes University undertaking a ‘Quantitative Research Methods’ course as part of their degree offering. A mixed methods approach was used to investigate students’ attitudes towards statistics and quantitative research methods. The Survey of Attitudes Toward Statistics (SATS-36) (Schau, 2003) captured student’s attitudes towards statistics using a Likert Scale instrument; whereas detailed qualitative interviews accentuated findings from the SATS-36. Key quantitative findings from the SATS-36 including students’ perceptions of statistics being a difficult course as well as students having a low affect towards statistics are detailed. Key qualitative findings related to why students experience statistics anxiety such as students’ (1) fear of failing statistics, (2) The late introduction of statistics in the Psychology curriculum, and (3) The role of educator/s in alleviating or promoting feelings of statistics anxiety are noted. The significance of these findings as well as the contributions of the study to the teaching and learning of statistics and quantitative research methods courses at Rhodes University are explored, in light of other studies on the topic of statistics anxiety and attitudes towards statistics/ quantitative research methods.
- Full Text:
- Date Issued: 2020
A morphogenetic study of ESD inclusion in Namibia’s Senior Primary English curriculum: a case study of the Khomas Region
- Authors: Malua, Anelly Ndapewa
- Date: 2020
- Subjects: Sustanable development -- Study and teaching -- Namibia , Environmental education -- Namibia , English language -- Study and teaching (Elementary) -- Namibia , Schools -- Namibia -- Curricula , Social change
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/142865 , vital:38124
- Description: Education policies are designed to structure and direct the content and process of the education that citizens receive. This includes the advancement of the Education for Sustainable Development (ESD) policy in formal school curricula. If effectively implemented, ESD can be a significant educational intervention in southern African countries’ development trajectories. ESD incorporates the environmental, social and economic pillars of Sustainable Development and it has the potential to curb issues such as poverty reduction, climate change, biodiversity loss and unsustainable patterns of consumption and production. This qualitative case study investigated factors that have historically shaped, and currently shape, the emergence of ESD implementation in the Senior Primary English curriculum in the Khomas Region of Namibia. As a critical realist enquiry, the study sought to go beyond interpretations of the empirical to identify causal mechanisms in the domains of ‘the actual’ and ‘the real’. The study was guided by Margaret Archer’s theory of Morphogenesis / Morphostasis and her methodological tool of ‘analytical dualism’. The case record consisted of educational documents, teacher questionnaires and semistructured interviews. The morphogenetic approach highlighted how the implementation of ESD through the Senior Primary English curriculum from 1990 to 2018 was conditioned by the interplay of social and cultural structures and mechanisms and human agency, particularly teachers’ agency. The study revealed that although ESD implementation has emerged in the Senior Primary English curriculum, its emergence is not synchronous with the structural and agential entities. The findings point to a policy-structure mismatch which has relevance for policy makers, practitioners and other ESD stakeholders. The significance of this study is that it stands to fill a research gap regarding ESD implementation in Namibia’s Senior Primary English curriculum. The study makes recommendations for tangible ways to strengthen ESD practice in Senior Primary English teaching in Namibia such as increasing professional development opportunities to orientate teachers to ESD, strengthening networks that can build teacher agency in relation to ESD, and promoting a theme-based approach to ESD practice in English Language teaching.
- Full Text:
- Date Issued: 2020
- Authors: Malua, Anelly Ndapewa
- Date: 2020
- Subjects: Sustanable development -- Study and teaching -- Namibia , Environmental education -- Namibia , English language -- Study and teaching (Elementary) -- Namibia , Schools -- Namibia -- Curricula , Social change
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/142865 , vital:38124
- Description: Education policies are designed to structure and direct the content and process of the education that citizens receive. This includes the advancement of the Education for Sustainable Development (ESD) policy in formal school curricula. If effectively implemented, ESD can be a significant educational intervention in southern African countries’ development trajectories. ESD incorporates the environmental, social and economic pillars of Sustainable Development and it has the potential to curb issues such as poverty reduction, climate change, biodiversity loss and unsustainable patterns of consumption and production. This qualitative case study investigated factors that have historically shaped, and currently shape, the emergence of ESD implementation in the Senior Primary English curriculum in the Khomas Region of Namibia. As a critical realist enquiry, the study sought to go beyond interpretations of the empirical to identify causal mechanisms in the domains of ‘the actual’ and ‘the real’. The study was guided by Margaret Archer’s theory of Morphogenesis / Morphostasis and her methodological tool of ‘analytical dualism’. The case record consisted of educational documents, teacher questionnaires and semistructured interviews. The morphogenetic approach highlighted how the implementation of ESD through the Senior Primary English curriculum from 1990 to 2018 was conditioned by the interplay of social and cultural structures and mechanisms and human agency, particularly teachers’ agency. The study revealed that although ESD implementation has emerged in the Senior Primary English curriculum, its emergence is not synchronous with the structural and agential entities. The findings point to a policy-structure mismatch which has relevance for policy makers, practitioners and other ESD stakeholders. The significance of this study is that it stands to fill a research gap regarding ESD implementation in Namibia’s Senior Primary English curriculum. The study makes recommendations for tangible ways to strengthen ESD practice in Senior Primary English teaching in Namibia such as increasing professional development opportunities to orientate teachers to ESD, strengthening networks that can build teacher agency in relation to ESD, and promoting a theme-based approach to ESD practice in English Language teaching.
- Full Text:
- Date Issued: 2020
A multinational study of authors’ perceptions of and practical approaches to writing geography textbooks:
- Lee, Jongwon, Catling, Simon, Kidman, Gillian, Bednarz, Robert, Krause, Uwe, Martija, Andoni A, Ohnishi, Koji, Wilmot, P Dianne, Zecha, Stefane
- Authors: Lee, Jongwon , Catling, Simon , Kidman, Gillian , Bednarz, Robert , Krause, Uwe , Martija, Andoni A , Ohnishi, Koji , Wilmot, P Dianne , Zecha, Stefane
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158269 , vital:40168 , https://doi.org/10.1080/10382046.2020.1743931
- Description: Like all school textbooks, geography textbooks are authored. Almost all textbook research in and beyond geography has neglected their authors. This questionnaire-based multinational research project investigated the perceptions of 71 primary and secondary school geography textbook authors from seven countries in five continents. Though not intended as a definitive study, the findings identified several values and areas of expertise which many textbook authors in different countries held to be important for their work.
- Full Text:
- Date Issued: 2020
- Authors: Lee, Jongwon , Catling, Simon , Kidman, Gillian , Bednarz, Robert , Krause, Uwe , Martija, Andoni A , Ohnishi, Koji , Wilmot, P Dianne , Zecha, Stefane
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158269 , vital:40168 , https://doi.org/10.1080/10382046.2020.1743931
- Description: Like all school textbooks, geography textbooks are authored. Almost all textbook research in and beyond geography has neglected their authors. This questionnaire-based multinational research project investigated the perceptions of 71 primary and secondary school geography textbook authors from seven countries in five continents. Though not intended as a definitive study, the findings identified several values and areas of expertise which many textbook authors in different countries held to be important for their work.
- Full Text:
- Date Issued: 2020
A new approach to the biological monitoring of freshwater systems: Mapping nutrient loading in two South African rivers, a case study
- Motitsoe, Samuel N, Hill, Martin P, Avery, Trevor S, Hill, Jaclyn M
- Authors: Motitsoe, Samuel N , Hill, Martin P , Avery, Trevor S , Hill, Jaclyn M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444480 , vital:74244 , https://doi.org/10.1016/j.watres.2019.115391
- Description: Excessive addition of nitrogen (N) has threatened aquatic ecosystems for decades. Traditional water quality and biological monitoring assessment tools are widely used for monitoring nutrient loads and ecosystem health, but most of these methods cannot distinguish between different types and sources of pollution. This is a challenge, particularly when dealing with non-point sources of anthropogenic nitrogen inputs into freshwater systems. Recent laboratory studies using stable isotopic ratios (δ15N and C/N) of aquatic macrophytes (duckweed: Spirodela spp.) have shown successful differentiation and mapping between different N-sources and further, showed abilities to act as early warning indicators for environmental N-loading. Therefore, the aim of this study was to field test the potential of stable isotopic values of transplanted Spirodela spp. to map temporal and spatial N-loading variation and determine the main sources of N-loading in two river systems in the Eastern Cape Province of South Africa, using previously grown, isotopically calibrated and transplanted Spirodela plants, collected over a 13-month sampling period.
- Full Text:
- Date Issued: 2020
- Authors: Motitsoe, Samuel N , Hill, Martin P , Avery, Trevor S , Hill, Jaclyn M
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444480 , vital:74244 , https://doi.org/10.1016/j.watres.2019.115391
- Description: Excessive addition of nitrogen (N) has threatened aquatic ecosystems for decades. Traditional water quality and biological monitoring assessment tools are widely used for monitoring nutrient loads and ecosystem health, but most of these methods cannot distinguish between different types and sources of pollution. This is a challenge, particularly when dealing with non-point sources of anthropogenic nitrogen inputs into freshwater systems. Recent laboratory studies using stable isotopic ratios (δ15N and C/N) of aquatic macrophytes (duckweed: Spirodela spp.) have shown successful differentiation and mapping between different N-sources and further, showed abilities to act as early warning indicators for environmental N-loading. Therefore, the aim of this study was to field test the potential of stable isotopic values of transplanted Spirodela spp. to map temporal and spatial N-loading variation and determine the main sources of N-loading in two river systems in the Eastern Cape Province of South Africa, using previously grown, isotopically calibrated and transplanted Spirodela plants, collected over a 13-month sampling period.
- Full Text:
- Date Issued: 2020
A new genus and three new species of South African Cicadettini (Hemiptera Cicadidae Cicadettinae).
- Sanborn, Allen F, Villet, Martin H
- Authors: Sanborn, Allen F , Villet, Martin H
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441261 , vital:73871 , https://www.mapress.com/zt/article/view/zootaxa.4885.4.7
- Description: Ingcainyenzane irhiniensis n. gen., n. sp. and Ingcainyenzane nolukhanyoensis n. gen., n. sp. are described from Eastern Cape and Ingcainyenzane umgeniensis n. gen., n. sp. is described from KwaZulu-Natal, South Africa. Notes on its biology of the species and a key to species of the genus are also provided.
- Full Text:
- Date Issued: 2020
- Authors: Sanborn, Allen F , Villet, Martin H
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441261 , vital:73871 , https://www.mapress.com/zt/article/view/zootaxa.4885.4.7
- Description: Ingcainyenzane irhiniensis n. gen., n. sp. and Ingcainyenzane nolukhanyoensis n. gen., n. sp. are described from Eastern Cape and Ingcainyenzane umgeniensis n. gen., n. sp. is described from KwaZulu-Natal, South Africa. Notes on its biology of the species and a key to species of the genus are also provided.
- Full Text:
- Date Issued: 2020
A new indole alkaloid and other constituents from Monodora minor and Uvaria tanzaniae: their antitrypanosomal and antiplasmodial evaluation
- Christopher, Robert, Mgani, Quintino A, Nyandoro, Stephen S, Rousseau, Amanda L, Isaacs, Michelle, Hoppe, Heinrich C
- Authors: Christopher, Robert , Mgani, Quintino A , Nyandoro, Stephen S , Rousseau, Amanda L , Isaacs, Michelle , Hoppe, Heinrich C
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429347 , vital:72603 , xlink:href="https://doi.org/10.1080/14786419.2019.1710705"
- Description: Phytochemical investigation of the methanolic extract of Monodora minor Engl. and Diels (Annonaceae) stem bark yielded a new indole (E)-4-(1H-indol-5-yl)-but-3-en-2-one (1), a known indole 5-formyl-1H-indole (2) and an ubiquitous steroid sitosterol (3). The investigations of the methanolic extract of Uvaria tanzaniae Verdc. (Annonaceae) root bark yielded two previously reported C-benzylated dihydrochalcones namely uvaretin (4) and diuvaretin (5). Structures of the isolated compounds were elucidated based on NMR spectroscopy and high resolution electron ionization mass spectrometry (HR-EI-MS) data. All compounds were tested against Trypanosoma brucei brucei and Plasmodium falciparum. At a single concentration (20 μM) in the antitrypanosomal and antiplasmodial assays, compound 4 exhibited remarkable activities against T. brucei brucei and P. falciparum with percentage inhibition of 97.3% and 83.0% respectively, whereas compounds 1, 2, 3 and 5 were inactive. In a dose response antiplasmodial assay compound 4 exhibited moderate activity against P. falciparum with an IC50 value of 7.20 μM.
- Full Text:
- Date Issued: 2020
- Authors: Christopher, Robert , Mgani, Quintino A , Nyandoro, Stephen S , Rousseau, Amanda L , Isaacs, Michelle , Hoppe, Heinrich C
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429347 , vital:72603 , xlink:href="https://doi.org/10.1080/14786419.2019.1710705"
- Description: Phytochemical investigation of the methanolic extract of Monodora minor Engl. and Diels (Annonaceae) stem bark yielded a new indole (E)-4-(1H-indol-5-yl)-but-3-en-2-one (1), a known indole 5-formyl-1H-indole (2) and an ubiquitous steroid sitosterol (3). The investigations of the methanolic extract of Uvaria tanzaniae Verdc. (Annonaceae) root bark yielded two previously reported C-benzylated dihydrochalcones namely uvaretin (4) and diuvaretin (5). Structures of the isolated compounds were elucidated based on NMR spectroscopy and high resolution electron ionization mass spectrometry (HR-EI-MS) data. All compounds were tested against Trypanosoma brucei brucei and Plasmodium falciparum. At a single concentration (20 μM) in the antitrypanosomal and antiplasmodial assays, compound 4 exhibited remarkable activities against T. brucei brucei and P. falciparum with percentage inhibition of 97.3% and 83.0% respectively, whereas compounds 1, 2, 3 and 5 were inactive. In a dose response antiplasmodial assay compound 4 exhibited moderate activity against P. falciparum with an IC50 value of 7.20 μM.
- Full Text:
- Date Issued: 2020
A novel dimeric exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis
- Mafa, Mpho S, Dirr, Heinrich, Malgas, Samkelo, Krause, Rui W M, Pletschke, Brett I
- Authors: Mafa, Mpho S , Dirr, Heinrich , Malgas, Samkelo , Krause, Rui W M , Pletschke, Brett I
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193976 , vital:45412 , xlink:href="https://doi.org/10.3390/molecules25030746"
- Description: An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis. The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively). They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s−1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s−1. The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils. Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides. These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography–mass spectrometry (LC-MS). We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.
- Full Text:
- Date Issued: 2020
- Authors: Mafa, Mpho S , Dirr, Heinrich , Malgas, Samkelo , Krause, Rui W M , Pletschke, Brett I
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193976 , vital:45412 , xlink:href="https://doi.org/10.3390/molecules25030746"
- Description: An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis. The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively). They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s−1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s−1. The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils. Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides. These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography–mass spectrometry (LC-MS). We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.
- Full Text:
- Date Issued: 2020
A novel, improved throughput bioassay for determining the delative speed of antimalarial drug action using fluorescent vitality probes
- Authors: Laming, Dustin
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Treatment -- Africa , Antimalarials , Malaria vaccine
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/139902 , vital:37810
- Description: Malaria is one of the most prevalent diseases in Africa and Plasmodium falciparum is widely accepted as the most virulent of the malaria parasite species, with a fatality rate of 15 – 20 % of reported cases of infection. While various treatments have been accepted into early stage clinical trials, there has been little progress towards a proven vaccine. Pending a long-term solution, endemic countries rely heavily on the development of innovative drugs that are not only efficacious but are also quick acting. Traditional methods of evaluating antimalarial killing speeds via morphological assessments are inherently flawed by tedious, subjective interpretations of the heterogenous parasite morphology encountered in routine parasite culture conditions. This has led to the introduction of alternative assay formats to determine how rapidly compounds act on parasites in vitro: a parasite reduction ratio (PRR) assay that measures the recovery of parasite cultures from drug exposure; determining the shift in IC50 values of compounds when dose-response assays are carried out for different time periods; a bioluminescence relative rate of kill (BRRoK) assay that compares the extent to which compounds reduce firefly luciferase activity in transgenic parasites. Recent whole cell in vitro screening efforts have resulted in the generation of chemically diverse compound libraries such as the Medicines for Malaria Venture’s Pathogen Box, which houses 125 novel compounds with in vitro antiplasmodial activity. Assessing the relative killing speeds of these compounds would aid prioritizing fast-acting compounds that can be exploited as starting points for further development. This study aimed to develop a bioassay using the calcein-acetoxymethyl and propidium iodide fluorescent vitality probes, which would allow the relative speed of drug action on Plasmodium falciparum malaria parasites to be assessed and ranked in relation to each other using a quantitative, improved throughput approach. Initially applied to human (HeLa) cells, the assay was used to compare the relative speeds of action of 3 potential anti-cancer compounds by fluorescence microscopy. Subsequently adapted to P. falciparum, the assay was able to rank the relative speeds of action of standard antimalarials by fluorescence microscopy and two flow cytometry formats. Application of a multiwell flow cytometer increased throughput and enabled the assessment of experimental compounds, which included a set of artemisinin analogs and 125 antimalarial compounds in the MMV Pathogen Box. The latter culminated in the identification of five rapidly parasiticidal compounds in relation to the other compounds in the library, which may act as benchmark references for future studies and form the basis of the next generation of fast acting antimalarials that could be used to combat modern, resistant malaria.
- Full Text:
- Date Issued: 2020
- Authors: Laming, Dustin
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Treatment -- Africa , Antimalarials , Malaria vaccine
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/139902 , vital:37810
- Description: Malaria is one of the most prevalent diseases in Africa and Plasmodium falciparum is widely accepted as the most virulent of the malaria parasite species, with a fatality rate of 15 – 20 % of reported cases of infection. While various treatments have been accepted into early stage clinical trials, there has been little progress towards a proven vaccine. Pending a long-term solution, endemic countries rely heavily on the development of innovative drugs that are not only efficacious but are also quick acting. Traditional methods of evaluating antimalarial killing speeds via morphological assessments are inherently flawed by tedious, subjective interpretations of the heterogenous parasite morphology encountered in routine parasite culture conditions. This has led to the introduction of alternative assay formats to determine how rapidly compounds act on parasites in vitro: a parasite reduction ratio (PRR) assay that measures the recovery of parasite cultures from drug exposure; determining the shift in IC50 values of compounds when dose-response assays are carried out for different time periods; a bioluminescence relative rate of kill (BRRoK) assay that compares the extent to which compounds reduce firefly luciferase activity in transgenic parasites. Recent whole cell in vitro screening efforts have resulted in the generation of chemically diverse compound libraries such as the Medicines for Malaria Venture’s Pathogen Box, which houses 125 novel compounds with in vitro antiplasmodial activity. Assessing the relative killing speeds of these compounds would aid prioritizing fast-acting compounds that can be exploited as starting points for further development. This study aimed to develop a bioassay using the calcein-acetoxymethyl and propidium iodide fluorescent vitality probes, which would allow the relative speed of drug action on Plasmodium falciparum malaria parasites to be assessed and ranked in relation to each other using a quantitative, improved throughput approach. Initially applied to human (HeLa) cells, the assay was used to compare the relative speeds of action of 3 potential anti-cancer compounds by fluorescence microscopy. Subsequently adapted to P. falciparum, the assay was able to rank the relative speeds of action of standard antimalarials by fluorescence microscopy and two flow cytometry formats. Application of a multiwell flow cytometer increased throughput and enabled the assessment of experimental compounds, which included a set of artemisinin analogs and 125 antimalarial compounds in the MMV Pathogen Box. The latter culminated in the identification of five rapidly parasiticidal compounds in relation to the other compounds in the library, which may act as benchmark references for future studies and form the basis of the next generation of fast acting antimalarials that could be used to combat modern, resistant malaria.
- Full Text:
- Date Issued: 2020