Erasure: A challenge to Feminist and Queer research
- Marx, Jacqueline, Macleod, Catriona I
- Authors: Marx, Jacqueline , Macleod, Catriona I
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434210 , vital:73039 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_20
- Description: Anonymity and confidentiality feature prominently in research ethics guidelines. In this chapter we draw on examples from a research ethics application for a project involving women who had extricated themselves from relationships in which they had experienced intimate partner violence, and an ethnographic study of cross-dressing and drag, to illustrate the multiple ways in which identity masking can be put to work, both promoting and undermining what it means to do ethical research. We argue that the requirement for anonymity and confidentiality cannot be assessed without taking into account historicity and the sociopolitical contexts in which a study and its participants are located. The chapter concludes by giving consideration to the potential of a situated ethics approach and the implications for ethics review processes.
- Full Text:
- Date Issued: 2018
- Authors: Marx, Jacqueline , Macleod, Catriona I
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434210 , vital:73039 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_20
- Description: Anonymity and confidentiality feature prominently in research ethics guidelines. In this chapter we draw on examples from a research ethics application for a project involving women who had extricated themselves from relationships in which they had experienced intimate partner violence, and an ethnographic study of cross-dressing and drag, to illustrate the multiple ways in which identity masking can be put to work, both promoting and undermining what it means to do ethical research. We argue that the requirement for anonymity and confidentiality cannot be assessed without taking into account historicity and the sociopolitical contexts in which a study and its participants are located. The chapter concludes by giving consideration to the potential of a situated ethics approach and the implications for ethics review processes.
- Full Text:
- Date Issued: 2018
Escalating debt in municipalities: a case study of financial sustainability in the Amahlathi Municipality
- Authors: Mashiyi, Buntu
- Date: 2018
- Subjects: Municipal finance -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape -- Finance Debt
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22651 , vital:30045
- Description: Municipalities have recently been in the media for the wrong reasons with certain allegations being levelled against these institutions. The allegations range from lack of service delivery, corruption and lack of good governance. This has resulted in communities showing frustrations through protests that result in the destruction of the existing infrastructure and, in some areas, boycotts towards payments of rates and services. The study seeks to establish the reasons that lead to ratepayers and communities not paying for rates and services. In the process of establishing the above, it seeks to come up with suggestions and solutions that can be implemented by municipalities to ensure that the ‘going concern’ is addressed. To achieve the above purpose a literature review was conducted on available sources of information looking at financial sustainability, the role of municipalities, powers and functions of municipalities and how these institutions are funded. The study also looked at literature regarding the strategies to increase revenue and improve debt collection. The study used Amahlathi Local Municipality as a case study where a sample was selected from the population. Questionnaires were developed and distributed to the sample and responses were received to the tune of 203 respondents. Questionnaires were both qualitative and quantitative as they had both open-ended and closed questions. Quantitative questionnaires were distributed to the community and the qualitative questionnaires were distributed for expert opinion. Analysis was done against the research questions to establish if there were any links to the literature and the findings of the study. The study found that communities are fed up with unsustainable or a lack of service delivery that the municipality is providing. This has been linked to some information gaps between the councillors and the community in terms of municipal programmes. More needs to be done in terms of ensuring that ward meetings are held on a regular basis so as to improve communication. There is also an observation of a ‘culture of non-payment’ emanating from political canvassing during voting periods which negatively affects the cash flows of the municipality and that public representatives need to champion debt collection endeavours.
- Full Text:
- Date Issued: 2018
- Authors: Mashiyi, Buntu
- Date: 2018
- Subjects: Municipal finance -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape -- Finance Debt
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22651 , vital:30045
- Description: Municipalities have recently been in the media for the wrong reasons with certain allegations being levelled against these institutions. The allegations range from lack of service delivery, corruption and lack of good governance. This has resulted in communities showing frustrations through protests that result in the destruction of the existing infrastructure and, in some areas, boycotts towards payments of rates and services. The study seeks to establish the reasons that lead to ratepayers and communities not paying for rates and services. In the process of establishing the above, it seeks to come up with suggestions and solutions that can be implemented by municipalities to ensure that the ‘going concern’ is addressed. To achieve the above purpose a literature review was conducted on available sources of information looking at financial sustainability, the role of municipalities, powers and functions of municipalities and how these institutions are funded. The study also looked at literature regarding the strategies to increase revenue and improve debt collection. The study used Amahlathi Local Municipality as a case study where a sample was selected from the population. Questionnaires were developed and distributed to the sample and responses were received to the tune of 203 respondents. Questionnaires were both qualitative and quantitative as they had both open-ended and closed questions. Quantitative questionnaires were distributed to the community and the qualitative questionnaires were distributed for expert opinion. Analysis was done against the research questions to establish if there were any links to the literature and the findings of the study. The study found that communities are fed up with unsustainable or a lack of service delivery that the municipality is providing. This has been linked to some information gaps between the councillors and the community in terms of municipal programmes. More needs to be done in terms of ensuring that ward meetings are held on a regular basis so as to improve communication. There is also an observation of a ‘culture of non-payment’ emanating from political canvassing during voting periods which negatively affects the cash flows of the municipality and that public representatives need to champion debt collection endeavours.
- Full Text:
- Date Issued: 2018
Espoused and actual employee value proposition (EVP) at the SPAR Group - Eastern Cape (SPAR EC)
- Authors: Prins, Alveno
- Date: 2018
- Subjects: Employee retention -- South Africa -- Eastern Cape , Employee motivation -- South Africa -- Eastern Cape Personnel management -- South Africa -- Eastern Cape Supermarkets -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/23001 , vital:30291
- Description: Globally there is a shortage of talent with organisations facing the challenge of attracting, engaging and retaining top talent. It has therefore become a priority for any organisation to ensure, that the offer made to attract talent, extends beyond a salary. The Employee Value Proposition (EVP) is a strategic tool that could be used to ensure that the offer made to employees is indeed an offer of value, considering both extrinsic and intrinsic attributes; therefore, ensuring the attraction, engagement and retention of talent. All organisations have an EVP, whether documented or not; however, the effectiveness thereof should be questioned. This was the aim of this study, namely, to assess whether a gap exists between the espoused and actual employee value proposition at the SPAR Group – Eastern Cape (SPAR EC). The study used an existing questionnaire to collect data from a sample of 150 respondents. The questionnaire was adapted to align to the company and industry studied and aimed to answer the following questions: To what extent did employees expect to find, both intrinsic and extrinsic EVP characteristics in the company? How important were these intrinsic and extrinsic EVP characteristics to attract employees to the organisation? In addition, to what extent did employees experience the expected intrinsic and extrinsic EVP characteristics in the organisation? These questions formed the essence of the study, using expectation, importance and experience as categories to distinguish the perceptions of respondents. The data was analysed using descriptive and inferential statistics. The results indicated that a gap does indeed exist between the espoused and actual EVP at SPAR EC. EVP attributes associated with people and their advancement, such as personal and career development opportunities, recognition provided to employees for exceptional performance, work-life balance and the level of involvement and empowerment of employees, were experienced to a lower extent in the organisation, when compared to the respondents’ level of expectation and how important they deem these attributes. It was therefore recommended that SPAR EC focus on these attributes, as employees determine for themselves the extent to which they experience “value” in organisations. Employees who view their organisations' EVP to be of less value are likely to withdraw, by either not contributing or by resigning from the organisation altogether (Heger, 2007).
- Full Text:
- Date Issued: 2018
- Authors: Prins, Alveno
- Date: 2018
- Subjects: Employee retention -- South Africa -- Eastern Cape , Employee motivation -- South Africa -- Eastern Cape Personnel management -- South Africa -- Eastern Cape Supermarkets -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/23001 , vital:30291
- Description: Globally there is a shortage of talent with organisations facing the challenge of attracting, engaging and retaining top talent. It has therefore become a priority for any organisation to ensure, that the offer made to attract talent, extends beyond a salary. The Employee Value Proposition (EVP) is a strategic tool that could be used to ensure that the offer made to employees is indeed an offer of value, considering both extrinsic and intrinsic attributes; therefore, ensuring the attraction, engagement and retention of talent. All organisations have an EVP, whether documented or not; however, the effectiveness thereof should be questioned. This was the aim of this study, namely, to assess whether a gap exists between the espoused and actual employee value proposition at the SPAR Group – Eastern Cape (SPAR EC). The study used an existing questionnaire to collect data from a sample of 150 respondents. The questionnaire was adapted to align to the company and industry studied and aimed to answer the following questions: To what extent did employees expect to find, both intrinsic and extrinsic EVP characteristics in the company? How important were these intrinsic and extrinsic EVP characteristics to attract employees to the organisation? In addition, to what extent did employees experience the expected intrinsic and extrinsic EVP characteristics in the organisation? These questions formed the essence of the study, using expectation, importance and experience as categories to distinguish the perceptions of respondents. The data was analysed using descriptive and inferential statistics. The results indicated that a gap does indeed exist between the espoused and actual EVP at SPAR EC. EVP attributes associated with people and their advancement, such as personal and career development opportunities, recognition provided to employees for exceptional performance, work-life balance and the level of involvement and empowerment of employees, were experienced to a lower extent in the organisation, when compared to the respondents’ level of expectation and how important they deem these attributes. It was therefore recommended that SPAR EC focus on these attributes, as employees determine for themselves the extent to which they experience “value” in organisations. Employees who view their organisations' EVP to be of less value are likely to withdraw, by either not contributing or by resigning from the organisation altogether (Heger, 2007).
- Full Text:
- Date Issued: 2018
Essence of home: relevance of home and the assertion of place amongst Centane migrants, South Africa
- Authors: Njwambe, Avela Thandisiwe
- Date: 2018
- Subjects: Rural-urban migration -- South Africa , Migrant labor -- South Africa , Immigrants -- South Africa -- Social conditions , Immigrants -- South Africa -- Eastern Cape , Immigrants -- South Africa -- Homelands , Homelands (South Africa) , Home -- Psychological aspects
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/51866 , vital:26136
- Description: South Africa is currently experiencing ever-increasing rural-urban migration with many citizens from the former homeland areas migrating to cities to seek employment. Despite long-term residence in urban areas, many township dwellers do not consider these places to be home. Research into circular migration patterns reveal the lifelong relationships that migrants (amagoduka) have with their family home (ekhayeni). This study aimed to explore this relationship, looking in particular at the meanings imbued in the locality of home. In addition, the role of natural landscapes and social components in constructing meanings and attachments to ekhayeni for Xhosa-speaking migrants in Cape Town townships, who have family linkages to rural villages in the Transkei, was also explored. The study found that the landscape of home remains central to migrants’ cultural identity, belonging and well-being. Childhood experiences in nature, and cultural and recreational activities that continue to take rural inhabitants into these landscapes, remain key to this relationship. The rural area, as a geographical entity embodied with social and cultural/spiritual components continued to supply and satisfy many human needs for migrants, which were seen as crucial for psychological, mental and spiritual well-being.
- Full Text:
- Date Issued: 2018
Essence of home: relevance of home and the assertion of place amongst Centane migrants, South Africa
- Authors: Njwambe, Avela Thandisiwe
- Date: 2018
- Subjects: Rural-urban migration -- South Africa , Migrant labor -- South Africa , Immigrants -- South Africa -- Social conditions , Immigrants -- South Africa -- Eastern Cape , Immigrants -- South Africa -- Homelands , Homelands (South Africa) , Home -- Psychological aspects
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/51866 , vital:26136
- Description: South Africa is currently experiencing ever-increasing rural-urban migration with many citizens from the former homeland areas migrating to cities to seek employment. Despite long-term residence in urban areas, many township dwellers do not consider these places to be home. Research into circular migration patterns reveal the lifelong relationships that migrants (amagoduka) have with their family home (ekhayeni). This study aimed to explore this relationship, looking in particular at the meanings imbued in the locality of home. In addition, the role of natural landscapes and social components in constructing meanings and attachments to ekhayeni for Xhosa-speaking migrants in Cape Town townships, who have family linkages to rural villages in the Transkei, was also explored. The study found that the landscape of home remains central to migrants’ cultural identity, belonging and well-being. Childhood experiences in nature, and cultural and recreational activities that continue to take rural inhabitants into these landscapes, remain key to this relationship. The rural area, as a geographical entity embodied with social and cultural/spiritual components continued to supply and satisfy many human needs for migrants, which were seen as crucial for psychological, mental and spiritual well-being.
- Full Text:
- Date Issued: 2018
Establishing opportunities for using big data analysis at the Herald
- Authors: Joshua, Nadeem
- Date: 2018
- Subjects: Big data , Business intelligence -- Data processing Data mining
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/30529 , vital:30957
- Description: A few years ago, merely mentioning the term ‘big data’ within industry circles, would more than likely have received a quirky and confused look; however, the term big data has gained huge popularity in recent years among IT professionals and academics. The big data phenomenon has exploded in popularity worldwide, and continues to grow exponentially with each passing day. It has been good news for many industries, as industries are going ablaze with the huge volume, variety and velocity of data. As technology advances it is lifting and removing so many boundaries, and answering questions that are not currently being asked. Therefore, it is that big data is taking the world by storm, and it is safe to say that big data has gone mainstream with countless benefits being developed within industries. The opportunity for employing big data strategies are many, according to McKinsey and Company, and the growth in big data will spark a new wave of ‘innovation, competition and productivity’ within businesses (McKinsey & Company, 2011). Taking advantage of these opportunities will be challenging for companies, creating the need for new skills, tools and ways of thinking. Implementing big data would help in creating new innovative business models, as executives are challenged to make their organisations resilient and agile in today’s challenging business environment. This research paper aimed to unpack the understanding of big data, the challenges, and the value to an organisation and provide a guideline or framework to implement a big data strategy. Furthermore, this research examines the opportunities and the potential value that organisations would obtain from implementing big data, as well as the challenges that could hinder implementation. Due to the rapid growth and size of data, decision-makers need to be able to gain valuable insights from such varied and rapidly changing data that will help organisations make far better, intelligent and data-driven decisions which may help in improving operations.
- Full Text:
- Date Issued: 2018
- Authors: Joshua, Nadeem
- Date: 2018
- Subjects: Big data , Business intelligence -- Data processing Data mining
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/30529 , vital:30957
- Description: A few years ago, merely mentioning the term ‘big data’ within industry circles, would more than likely have received a quirky and confused look; however, the term big data has gained huge popularity in recent years among IT professionals and academics. The big data phenomenon has exploded in popularity worldwide, and continues to grow exponentially with each passing day. It has been good news for many industries, as industries are going ablaze with the huge volume, variety and velocity of data. As technology advances it is lifting and removing so many boundaries, and answering questions that are not currently being asked. Therefore, it is that big data is taking the world by storm, and it is safe to say that big data has gone mainstream with countless benefits being developed within industries. The opportunity for employing big data strategies are many, according to McKinsey and Company, and the growth in big data will spark a new wave of ‘innovation, competition and productivity’ within businesses (McKinsey & Company, 2011). Taking advantage of these opportunities will be challenging for companies, creating the need for new skills, tools and ways of thinking. Implementing big data would help in creating new innovative business models, as executives are challenged to make their organisations resilient and agile in today’s challenging business environment. This research paper aimed to unpack the understanding of big data, the challenges, and the value to an organisation and provide a guideline or framework to implement a big data strategy. Furthermore, this research examines the opportunities and the potential value that organisations would obtain from implementing big data, as well as the challenges that could hinder implementation. Due to the rapid growth and size of data, decision-makers need to be able to gain valuable insights from such varied and rapidly changing data that will help organisations make far better, intelligent and data-driven decisions which may help in improving operations.
- Full Text:
- Date Issued: 2018
Establishment of “The South African Bioinformatics Student Council” and activity highlights:
- Rafael, Candice Nancy, Ambler, Jon, Niehaus, Antoinette, Ross, James, Tastan Bishop, Özlem
- Authors: Rafael, Candice Nancy , Ambler, Jon , Niehaus, Antoinette , Ross, James , Tastan Bishop, Özlem
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148250 , vital:38723 , https://doi.org/10.14806/ej.23.0.903
- Description: The South African Society for Bioinformatics1 (SASBi) was officially formed in September 2012 during a joint Congress with the South African Genetics Society (SAGS). Prior to this there was no official body to represent bioinformatic researchers and students in the country. The establishment of SASBi also led to the establishment of the Student Society as a platform for students to meet and discuss their research activities, but also to socialise and broaden their network of knowledge and friendships. A small group of students joined as volunteers to pioneer and set up a SASBi Student Council (SASBiSC). As a first step, one representative, selected from the attendees present at the first Joint Congress of SASBi and SAGS, was elected to the main SASBi Council.
- Full Text:
- Date Issued: 2018
- Authors: Rafael, Candice Nancy , Ambler, Jon , Niehaus, Antoinette , Ross, James , Tastan Bishop, Özlem
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148250 , vital:38723 , https://doi.org/10.14806/ej.23.0.903
- Description: The South African Society for Bioinformatics1 (SASBi) was officially formed in September 2012 during a joint Congress with the South African Genetics Society (SAGS). Prior to this there was no official body to represent bioinformatic researchers and students in the country. The establishment of SASBi also led to the establishment of the Student Society as a platform for students to meet and discuss their research activities, but also to socialise and broaden their network of knowledge and friendships. A small group of students joined as volunteers to pioneer and set up a SASBi Student Council (SASBiSC). As a first step, one representative, selected from the attendees present at the first Joint Congress of SASBi and SAGS, was elected to the main SASBi Council.
- Full Text:
- Date Issued: 2018
Estuarine microalgal bloom dynamics at multiple temporal scales
- Authors: Lemley, Daniel Alan
- Date: 2018
- Subjects: Ecological integrity -- South Africa , Phytoplankton populations -- South Africa , Estuarine ecology -- South Africa , Freshwater algae -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21044 , vital:29431
- Description: Microalgal communities sustain the ecological functioning of estuarine ecosystems through the provision of numerous ecosystem services. As such, gaining an understanding of the processes that govern spatio-temporal patterns of phytoplankton communities is key to the effective management of these ecosystems. However, detailed investigations of estuarine phytoplankton dynamics, and particularly harmful algal blooms (HAB), are largely absent in South Africa. Therefore, geared at addressing these knowledge gaps, this study was initiated to elucidate the processes shaping estuarine phytoplankton bloom dynamics at multiple temporal scales. Initially, five permanently open estuaries, each with varying types and levels of anthropogenic pressures, were selected to test the efficacy of an estuarine eutrophic condition index to flow variability (‘Annual’ study). Results from this study highlighted the sensitivity of the proposed index to environmental perturbations (e.g. flood events), whilst also identifying freshwater inflow variability and anthropogenic disturbance as the key processes shaping microalgal responses at an annual scale. Next, the agriculturally-influenced Gamtoos and Sundays estuaries were further investigated to elucidate the role of natural and anthropogenic drivers in defining microalgal responses (‘Seasonal’ study). Despite similarities in nutrient input and temperature patterns, phytoplankton bloom conditions (> 20 μg Chl-a l-1) were episodic and flow-dependent in the Gamtoos Estuary, whilst those in the Sundays Estuary were persistent and seasonal. Related to its reduced hydrodynamic variability – i.e. consistent nutrient-rich baseflows and reduced propensity for flushing events – persistent undesirable disturbances were highlighted for the Sundays Estuary, including: summer bottom-water hypoxia (< 2 mg l-1) and exceptional proliferations (> 550 μg Chl-a l-1) of two HAB species (Heterosigma akashiwo and Heterocapsa rotundata). Finally, fine-scale ecological research was undertaken in the Sundays Estuary to identify the processes – abiotic and biotic – that facilitate HABs (‘Daily’ and ‘Hourly’ studies). Findings from the ‘Daily’ study, identified inorganic nutrient availability (i.e. nitrate and phosphate) and mesohaline conditions (ca. 10) as the key bottom-up controls influencing the magnitude and duration of spring/summer phytoplankton blooms. Additionally, bottom-water hypoxia was explicitly linked to the decay of a single HAB species. During the ‘Hourly’ investigation, four known HAB-forming species were recorded at bloom concentrations. Model results indicated that variability in temperature, salinity profiles and nitrate concentrations were significant in facilitating the occurrence of HAB species. Finally, local biotic interactions (e.g. interspecies competition, diel vertical migration and mixotrophy) were recognized as key mechanisms shaping phytoplankton communities. The persistent occurrence of HABs is a new feature in South African estuaries and continued research is needed to recommend management responses. Ultimately, this research highlights the multitude of processes at work shaping phytoplankton variability in estuaries. From a broad perspective (i.e. seasonal and annual), processes such as freshwater inflow regimes, degree of anthropogenic disturbance, as well as seasonal temperature and nutrient supply patterns are the key processes. At a more refined scale (i.e. hourly and daily), local processes including salinity preferences, nutrient availability, diel light cycles and internal biotic interactions are the key drivers organising phytoplankton dynamics. Given the potentially severe ecological consequences of disrupting natural phytoplankton dynamics (e.g. HABs), an element of ‘unpredictability’ should be restored to the hydrological and chemical makeup of highly-regulated estuaries to prevent the continued exacerbation of eutrophic symptoms.
- Full Text:
- Date Issued: 2018
- Authors: Lemley, Daniel Alan
- Date: 2018
- Subjects: Ecological integrity -- South Africa , Phytoplankton populations -- South Africa , Estuarine ecology -- South Africa , Freshwater algae -- Ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/21044 , vital:29431
- Description: Microalgal communities sustain the ecological functioning of estuarine ecosystems through the provision of numerous ecosystem services. As such, gaining an understanding of the processes that govern spatio-temporal patterns of phytoplankton communities is key to the effective management of these ecosystems. However, detailed investigations of estuarine phytoplankton dynamics, and particularly harmful algal blooms (HAB), are largely absent in South Africa. Therefore, geared at addressing these knowledge gaps, this study was initiated to elucidate the processes shaping estuarine phytoplankton bloom dynamics at multiple temporal scales. Initially, five permanently open estuaries, each with varying types and levels of anthropogenic pressures, were selected to test the efficacy of an estuarine eutrophic condition index to flow variability (‘Annual’ study). Results from this study highlighted the sensitivity of the proposed index to environmental perturbations (e.g. flood events), whilst also identifying freshwater inflow variability and anthropogenic disturbance as the key processes shaping microalgal responses at an annual scale. Next, the agriculturally-influenced Gamtoos and Sundays estuaries were further investigated to elucidate the role of natural and anthropogenic drivers in defining microalgal responses (‘Seasonal’ study). Despite similarities in nutrient input and temperature patterns, phytoplankton bloom conditions (> 20 μg Chl-a l-1) were episodic and flow-dependent in the Gamtoos Estuary, whilst those in the Sundays Estuary were persistent and seasonal. Related to its reduced hydrodynamic variability – i.e. consistent nutrient-rich baseflows and reduced propensity for flushing events – persistent undesirable disturbances were highlighted for the Sundays Estuary, including: summer bottom-water hypoxia (< 2 mg l-1) and exceptional proliferations (> 550 μg Chl-a l-1) of two HAB species (Heterosigma akashiwo and Heterocapsa rotundata). Finally, fine-scale ecological research was undertaken in the Sundays Estuary to identify the processes – abiotic and biotic – that facilitate HABs (‘Daily’ and ‘Hourly’ studies). Findings from the ‘Daily’ study, identified inorganic nutrient availability (i.e. nitrate and phosphate) and mesohaline conditions (ca. 10) as the key bottom-up controls influencing the magnitude and duration of spring/summer phytoplankton blooms. Additionally, bottom-water hypoxia was explicitly linked to the decay of a single HAB species. During the ‘Hourly’ investigation, four known HAB-forming species were recorded at bloom concentrations. Model results indicated that variability in temperature, salinity profiles and nitrate concentrations were significant in facilitating the occurrence of HAB species. Finally, local biotic interactions (e.g. interspecies competition, diel vertical migration and mixotrophy) were recognized as key mechanisms shaping phytoplankton communities. The persistent occurrence of HABs is a new feature in South African estuaries and continued research is needed to recommend management responses. Ultimately, this research highlights the multitude of processes at work shaping phytoplankton variability in estuaries. From a broad perspective (i.e. seasonal and annual), processes such as freshwater inflow regimes, degree of anthropogenic disturbance, as well as seasonal temperature and nutrient supply patterns are the key processes. At a more refined scale (i.e. hourly and daily), local processes including salinity preferences, nutrient availability, diel light cycles and internal biotic interactions are the key drivers organising phytoplankton dynamics. Given the potentially severe ecological consequences of disrupting natural phytoplankton dynamics (e.g. HABs), an element of ‘unpredictability’ should be restored to the hydrological and chemical makeup of highly-regulated estuaries to prevent the continued exacerbation of eutrophic symptoms.
- Full Text:
- Date Issued: 2018
Ethics in critical research: Stories from the field
- Macleod, Catriona I, Marx, Jacqueline, Mnyaka, Phindezwa, Treharne, Gareth J
- Authors: Macleod, Catriona I , Marx, Jacqueline , Mnyaka, Phindezwa , Treharne, Gareth J
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434223 , vital:73040 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_1
- Description: In this chapter we introduce the approaches to ethics in critical research applied throughout this handbook. Critical research questions who benefits from research and offers critiques rooted in postmodern and liberatory theories, including feminism, Marxism, and postcolonialism. Authors of chapters in the handbook explore ethical issues faced when conducting critical research through stories from the field across a range of methodologies, disciplines, and locations. The chapter overviews the four sections of the handbook and the ethical challenges associated with conducting critical research within the bureaucracy of ethics committees and other systems of governance, blurring the boundaries between researchers and participants/co-researchers, giving voice through research whilst applying anonymity or naming participants/co-researchers, and conducting research with various configurations of power between researchers and participants/co-researchers.
- Full Text:
- Date Issued: 2018
- Authors: Macleod, Catriona I , Marx, Jacqueline , Mnyaka, Phindezwa , Treharne, Gareth J
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/434223 , vital:73040 , ISBN 978-3-319-74720-0 , https://link.springer.com/chapter/10.1007/978-3-319-74721-7_1
- Description: In this chapter we introduce the approaches to ethics in critical research applied throughout this handbook. Critical research questions who benefits from research and offers critiques rooted in postmodern and liberatory theories, including feminism, Marxism, and postcolonialism. Authors of chapters in the handbook explore ethical issues faced when conducting critical research through stories from the field across a range of methodologies, disciplines, and locations. The chapter overviews the four sections of the handbook and the ethical challenges associated with conducting critical research within the bureaucracy of ethics committees and other systems of governance, blurring the boundaries between researchers and participants/co-researchers, giving voice through research whilst applying anonymity or naming participants/co-researchers, and conducting research with various configurations of power between researchers and participants/co-researchers.
- Full Text:
- Date Issued: 2018
Ethics in transdisciplinary research: reflections on the implications of ‘Science with Society’
- Cockburn, Jessica J, Cundill, Georgina
- Authors: Cockburn, Jessica J , Cundill, Georgina
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/436187 , vital:73236 , ISBN 978-3-319-74721-7 , https://doi.org/10.1007/978-3-319-74721-7_6
- Description: Transdisciplinarity is a reflexive, participatory research approach that addresses societal problems by transcending the boundaries between science and society through knowledge co-production. Research ethics clearance procedures are of-ten out of step with such forms of engaged research. Through a case study of a transdisciplinary PhD, we share our experiences of the ethical challenges involved in research that takes place beyond the bounds of procedural ethics. Our research aims to co-produce knowledge on environmental stewardship. In this chapter we consider what strategies transdisciplinary scholars can employ to ensure they fulfil the requirements of ethical research in the absence of suitably aligned institutional processes, and what changes need to be made to research ethics clearance procedures to ensure they are able to account for transdisciplinary research.
- Full Text:
- Date Issued: 2018
- Authors: Cockburn, Jessica J , Cundill, Georgina
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , book chapter
- Identifier: http://hdl.handle.net/10962/436187 , vital:73236 , ISBN 978-3-319-74721-7 , https://doi.org/10.1007/978-3-319-74721-7_6
- Description: Transdisciplinarity is a reflexive, participatory research approach that addresses societal problems by transcending the boundaries between science and society through knowledge co-production. Research ethics clearance procedures are of-ten out of step with such forms of engaged research. Through a case study of a transdisciplinary PhD, we share our experiences of the ethical challenges involved in research that takes place beyond the bounds of procedural ethics. Our research aims to co-produce knowledge on environmental stewardship. In this chapter we consider what strategies transdisciplinary scholars can employ to ensure they fulfil the requirements of ethical research in the absence of suitably aligned institutional processes, and what changes need to be made to research ethics clearance procedures to ensure they are able to account for transdisciplinary research.
- Full Text:
- Date Issued: 2018
Ethnicity, conflict and the developmental state in Rwanda
- Authors: Muleya, Emmison
- Date: 2018
- Subjects: Rwanda -- Politics and government -- 1994- Rwanda -- Politics and government -- 1962-1994 Rwanda -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD (Development Studies)
- Identifier: http://hdl.handle.net/10353/10083 , vital:35334
- Description: Socioeconomic development is a crucial means of improving standards of living and increasing the incomes of developing countries. This study is premised upon the assumption that the concepts of the developmental state and socioeconomic development are inextricably interrelated. The concept of a developmental state has gained a significant amount of traction in both academic and non-academic circles in recent times. This study took the form of a case study of Rwanda and its developmental trajectory since the country gained independence in 1962. By adopting a historical approach, this study determined that the factors which continue to retard socioeconomic development in post-colonial and post-genocide Rwanda are rooted in a lingering colonial legacy. Although Rwanda has achieved a significant degree of socioeconomic development since 1994, the sustainability of the nascent developmental state which appears to be emerging could still be undermined by ethnic divisions. Accordingly, it was recommended on the basis of the findings of this study that the Rwandan state and its development partners should pursue transformative socioeconomic policies which ensure an equitable distribution of socioeconomic resources, in order to facilitate the creation of an enabling environment for local entrepreneurs and a reduction of dependence upon foreign aid through the effective mobilisation of domestic resources.
- Full Text:
- Date Issued: 2018
- Authors: Muleya, Emmison
- Date: 2018
- Subjects: Rwanda -- Politics and government -- 1994- Rwanda -- Politics and government -- 1962-1994 Rwanda -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , PhD (Development Studies)
- Identifier: http://hdl.handle.net/10353/10083 , vital:35334
- Description: Socioeconomic development is a crucial means of improving standards of living and increasing the incomes of developing countries. This study is premised upon the assumption that the concepts of the developmental state and socioeconomic development are inextricably interrelated. The concept of a developmental state has gained a significant amount of traction in both academic and non-academic circles in recent times. This study took the form of a case study of Rwanda and its developmental trajectory since the country gained independence in 1962. By adopting a historical approach, this study determined that the factors which continue to retard socioeconomic development in post-colonial and post-genocide Rwanda are rooted in a lingering colonial legacy. Although Rwanda has achieved a significant degree of socioeconomic development since 1994, the sustainability of the nascent developmental state which appears to be emerging could still be undermined by ethnic divisions. Accordingly, it was recommended on the basis of the findings of this study that the Rwandan state and its development partners should pursue transformative socioeconomic policies which ensure an equitable distribution of socioeconomic resources, in order to facilitate the creation of an enabling environment for local entrepreneurs and a reduction of dependence upon foreign aid through the effective mobilisation of domestic resources.
- Full Text:
- Date Issued: 2018
Eti zōsa phlox
- Authors: Van Schoor, David J
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/468457 , vital:77062 , https://www.jstor.org/stable/26945033?seq=1
- Description: Myths of the god typically recount a divine presence undiscerned or misdiagnosed by mortals, with tragic consequences. The vengeful Dionysus of Euripides’ Bacchae comes amongst humans disguised, ironically, to make them learn and recognise his divine identity and that they are in the presence of the truly, if not at first apparently, immortal. The kind of inference that it seems mortals are expected to rely upon in the vicinity of Dionysus in order to secure that all-important knowledge and recognition, is explored in this paper, which is offered as a contribution towards the development of a contemporary anthropology of Greek tragedy and its god. The anthropology of art and agency of Alfred Gell is mobilised in order to explore how different modes of inference are implicitly compared and evaluated in the drama and to suggest what this has to do with the nature of Dionysiac experience as represented by the tragic poet.
- Full Text:
- Date Issued: 2018
- Authors: Van Schoor, David J
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/468457 , vital:77062 , https://www.jstor.org/stable/26945033?seq=1
- Description: Myths of the god typically recount a divine presence undiscerned or misdiagnosed by mortals, with tragic consequences. The vengeful Dionysus of Euripides’ Bacchae comes amongst humans disguised, ironically, to make them learn and recognise his divine identity and that they are in the presence of the truly, if not at first apparently, immortal. The kind of inference that it seems mortals are expected to rely upon in the vicinity of Dionysus in order to secure that all-important knowledge and recognition, is explored in this paper, which is offered as a contribution towards the development of a contemporary anthropology of Greek tragedy and its god. The anthropology of art and agency of Alfred Gell is mobilised in order to explore how different modes of inference are implicitly compared and evaluated in the drama and to suggest what this has to do with the nature of Dionysiac experience as represented by the tragic poet.
- Full Text:
- Date Issued: 2018
Evaluating farmers' perceptions on climate variability and the impact of management practices on rangeland condition at Tukulu Farm, South Africa
- Authors: Huza, Siphamandla
- Date: 2018
- Subjects: Rangelands -- South Africa -- Eastern Cape Range management -- South Africa -- Eastern Cape Livestock -- Breeding -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/10586 , vital:35611
- Description: Rangelands are ecological systems largely used for extensive livestock production, which continually play a vital role in developing the economy of rural communities worldwide. These ecosystems also have the potential to provide vital secondary resources like medicinal plants, firewood and wild foods. This study assessed the impacts of management practices on rangeland condition at Tukulu communal farm in the Eastern Cape province of South Africa. Communal farmers’ perceptions were also investigated on vegetation change and awareness to climate variability. A structured questionnaire with open-ended and closed questions was used to interview communal farmers about several components of their rangeland, their awareness to climate variability and their household demographics were recorded. A rangeland condition assessment with the use of a three-tier system was used to determine vegetation condition and assessment of vegetal change over the past ten to twenty years. Botanical species composition, biomass yield, basal cover, woody plant density, browsing units and soil chemical composition were measured. Tukulu communal rangeland was demarcated into three homogenous vegetation units (HVU) namely, open grassland, scattered bushland and dense bushland. Sample sites of (100×50 m)2 with four replicates were constructed in each HVU. Herbaceous vegetation and basal cover were determined along 100 m transects in each sample site using a step-point method. Aboveground biomass production was estimated using a quadrat system following a systematic sampling by cutting all material within 0.25 m2 quadrats located along 100 m transects. Woody plant density (plants/ha), tree equivalents (TE/ha) and browsing unit (BU/ha) were estimated from the number of woody plants, total woody heights and the number of acceptable and available (<1.5 m) woody species. Soil sample analysis was conducted to determine OC, P, K, N, Ca, Mg, Na, Zn, Cu, and Mn and soil pH in all three HVUs. The respondents consisted of 65 percent males and 35 percent females with an average of about five persons per household. The study revealed that communal farmers at Tukulu were fairly knowledgeable about the changes that have occurred in their rangeland. All respondents 100 percent perceived that their rangeland had changed from good to poor condition. This was characterized by the shift from grass dominance to woody plant encroachment. These changes were linked to the perceived changes in climate. Farmers at Tukulu communal area were generally aware of the changes in climate, 95 percent of the respondents’ perceived changes in weather patterns such as changes in rain seasons, hot weather conditions, unpredictable rainfalls, drought, heavy winds and increased temperatures. The results revealed that Tukulu communal rangeland comprised of high species diversity. Twenty-one herbaceous species and 24 woody species were recorded at Tukulu rangeland. The grass species composition was characterised by 71 percent Increaser II species, 24 percent Decreaser and 5 percent Increaser I species. A highly palatable Decreaser species Themeda triandra decreased in abundance from open grassland to scattered bushland. There were no significant differences (p>0.05) in biomass yield in all three HVUs. However, scattered bushland 761.5 kg/ha and dense bushland 735.8 kg/ha had higher biomass yield when compared to open grassland 688.5 kg/ha. Bush encroachment was observed in dense bushland as the woody plant density 4487.5 trees/ha and tree equivalents 3451.3 trees/ha were significantly (P<0.05) higher than the recommended threshold standards of 2400 trees/ha and 2500 TE/ha. Scattered bushland had tree density of 1900 trees/ha and tree-equivalents of 1534.3 TE/ha. Soil macro and micro nutrients differed significantly between the different HVUs. The levels of Na, Ca, Mg, Cu, Mn differed significantly (p<0.05) between HVUs, increasing from open grassland to dense bushland. Organic carbon concentrations were higher under tree canopies in dense bushland. However, the levels of phosphorus and potassium were higher in open grassland than in the wooded areas. Soil pH in scattered bushland (5.3) and dense bushland (5.18) were significantly higher (p<0.05) than in open grassland (4.89). This study shows complications in management policies governing the Tukulu communal area. There is a need to merge both scientific and indigenous ecological knowledge to ensure the desired outcomes, as this could indeed offer some great insights for communal areas. Therefore, a rangeland rehabilitation programme should be implemented at Tukulu communal rangeland, which will address the issue of bush encroachment and implement a rotational grazing system by fencing camps that will be based on acceptable stocking rates.
- Full Text:
- Date Issued: 2018
- Authors: Huza, Siphamandla
- Date: 2018
- Subjects: Rangelands -- South Africa -- Eastern Cape Range management -- South Africa -- Eastern Cape Livestock -- Breeding -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/10586 , vital:35611
- Description: Rangelands are ecological systems largely used for extensive livestock production, which continually play a vital role in developing the economy of rural communities worldwide. These ecosystems also have the potential to provide vital secondary resources like medicinal plants, firewood and wild foods. This study assessed the impacts of management practices on rangeland condition at Tukulu communal farm in the Eastern Cape province of South Africa. Communal farmers’ perceptions were also investigated on vegetation change and awareness to climate variability. A structured questionnaire with open-ended and closed questions was used to interview communal farmers about several components of their rangeland, their awareness to climate variability and their household demographics were recorded. A rangeland condition assessment with the use of a three-tier system was used to determine vegetation condition and assessment of vegetal change over the past ten to twenty years. Botanical species composition, biomass yield, basal cover, woody plant density, browsing units and soil chemical composition were measured. Tukulu communal rangeland was demarcated into three homogenous vegetation units (HVU) namely, open grassland, scattered bushland and dense bushland. Sample sites of (100×50 m)2 with four replicates were constructed in each HVU. Herbaceous vegetation and basal cover were determined along 100 m transects in each sample site using a step-point method. Aboveground biomass production was estimated using a quadrat system following a systematic sampling by cutting all material within 0.25 m2 quadrats located along 100 m transects. Woody plant density (plants/ha), tree equivalents (TE/ha) and browsing unit (BU/ha) were estimated from the number of woody plants, total woody heights and the number of acceptable and available (<1.5 m) woody species. Soil sample analysis was conducted to determine OC, P, K, N, Ca, Mg, Na, Zn, Cu, and Mn and soil pH in all three HVUs. The respondents consisted of 65 percent males and 35 percent females with an average of about five persons per household. The study revealed that communal farmers at Tukulu were fairly knowledgeable about the changes that have occurred in their rangeland. All respondents 100 percent perceived that their rangeland had changed from good to poor condition. This was characterized by the shift from grass dominance to woody plant encroachment. These changes were linked to the perceived changes in climate. Farmers at Tukulu communal area were generally aware of the changes in climate, 95 percent of the respondents’ perceived changes in weather patterns such as changes in rain seasons, hot weather conditions, unpredictable rainfalls, drought, heavy winds and increased temperatures. The results revealed that Tukulu communal rangeland comprised of high species diversity. Twenty-one herbaceous species and 24 woody species were recorded at Tukulu rangeland. The grass species composition was characterised by 71 percent Increaser II species, 24 percent Decreaser and 5 percent Increaser I species. A highly palatable Decreaser species Themeda triandra decreased in abundance from open grassland to scattered bushland. There were no significant differences (p>0.05) in biomass yield in all three HVUs. However, scattered bushland 761.5 kg/ha and dense bushland 735.8 kg/ha had higher biomass yield when compared to open grassland 688.5 kg/ha. Bush encroachment was observed in dense bushland as the woody plant density 4487.5 trees/ha and tree equivalents 3451.3 trees/ha were significantly (P<0.05) higher than the recommended threshold standards of 2400 trees/ha and 2500 TE/ha. Scattered bushland had tree density of 1900 trees/ha and tree-equivalents of 1534.3 TE/ha. Soil macro and micro nutrients differed significantly between the different HVUs. The levels of Na, Ca, Mg, Cu, Mn differed significantly (p<0.05) between HVUs, increasing from open grassland to dense bushland. Organic carbon concentrations were higher under tree canopies in dense bushland. However, the levels of phosphorus and potassium were higher in open grassland than in the wooded areas. Soil pH in scattered bushland (5.3) and dense bushland (5.18) were significantly higher (p<0.05) than in open grassland (4.89). This study shows complications in management policies governing the Tukulu communal area. There is a need to merge both scientific and indigenous ecological knowledge to ensure the desired outcomes, as this could indeed offer some great insights for communal areas. Therefore, a rangeland rehabilitation programme should be implemented at Tukulu communal rangeland, which will address the issue of bush encroachment and implement a rotational grazing system by fencing camps that will be based on acceptable stocking rates.
- Full Text:
- Date Issued: 2018
Evaluating metabolism-induced toxicity using a non-hepatic cell line
- Authors: Weyers, Carli
- Date: 2018
- Subjects: Cytochrome P-450 , Drugs Metabolism , Drugs Design
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/61950 , vital:28087
- Description: The drug discovery pipeline is a complicated process taking roughly 15 years to complete, costing in excess of $1 billion per new chemical entity. It has been estimated that for every 100, 000 promising hit or lead compounds, only one will make it onto the market due to numerous drug candidates being discarded because of many complications. One such complication is metabolism-induced toxicity. Accordingly, an early understanding of the metabolism of any new chemical entity is becoming an integral part of the pipeline. In order to explore this, various methods have been developed including in silico and in vitro techniques. One such method involves performing cell viability assays on human liver cancer cell lines, which overexpress specific metabolic cytochrome P450 enzymes. If a toxic metabolite is produced it would result in reduced cell viability of the transformed cell line in comparison to a control. Since the liver is the primary site of metabolism in the human body, we were curious as to the extent to which background metabolism may play a role in the degree to which toxic metabolites would be produced in these cell lines. The aim of this project, therefore, was to establish if a non-hepatic cell-based system which overexpresses CYP3A4 could be used to detect the metabolism and any subsequent toxicity of compounds which have been reported to be substrates of the CYP450 enzyme. The HEK293 cell line was stably transfected with a plasmid vector for human CYP3A4 to create a model overexpression system for our metabolism studies. The activity of the enzyme was confirmed using the substrate, 7-benzyloxy-4-trifluoromethyl-coumarin. Subsequently, cytotoxicity testing was done on four known pharmaceuticals reported to generate toxic metabolites in hepatic cell-based assays. In silico metabolic predictions on the four known compounds were performed and compared to the results of published literature. Finally, the metabolism of one compound was studied using a combination of high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) in order to detect predicted metabolites. We observed no change in cellular toxicity nor did we detect the formation of metabolites, even though the overexpressed CYP3A4 enzyme was active. The results suggest that caution should be taken when interpreting the results of cell-based metabolism studies, and background metabolism may play a significant role in the data. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2018
- Full Text:
- Date Issued: 2018
- Authors: Weyers, Carli
- Date: 2018
- Subjects: Cytochrome P-450 , Drugs Metabolism , Drugs Design
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/61950 , vital:28087
- Description: The drug discovery pipeline is a complicated process taking roughly 15 years to complete, costing in excess of $1 billion per new chemical entity. It has been estimated that for every 100, 000 promising hit or lead compounds, only one will make it onto the market due to numerous drug candidates being discarded because of many complications. One such complication is metabolism-induced toxicity. Accordingly, an early understanding of the metabolism of any new chemical entity is becoming an integral part of the pipeline. In order to explore this, various methods have been developed including in silico and in vitro techniques. One such method involves performing cell viability assays on human liver cancer cell lines, which overexpress specific metabolic cytochrome P450 enzymes. If a toxic metabolite is produced it would result in reduced cell viability of the transformed cell line in comparison to a control. Since the liver is the primary site of metabolism in the human body, we were curious as to the extent to which background metabolism may play a role in the degree to which toxic metabolites would be produced in these cell lines. The aim of this project, therefore, was to establish if a non-hepatic cell-based system which overexpresses CYP3A4 could be used to detect the metabolism and any subsequent toxicity of compounds which have been reported to be substrates of the CYP450 enzyme. The HEK293 cell line was stably transfected with a plasmid vector for human CYP3A4 to create a model overexpression system for our metabolism studies. The activity of the enzyme was confirmed using the substrate, 7-benzyloxy-4-trifluoromethyl-coumarin. Subsequently, cytotoxicity testing was done on four known pharmaceuticals reported to generate toxic metabolites in hepatic cell-based assays. In silico metabolic predictions on the four known compounds were performed and compared to the results of published literature. Finally, the metabolism of one compound was studied using a combination of high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) in order to detect predicted metabolites. We observed no change in cellular toxicity nor did we detect the formation of metabolites, even though the overexpressed CYP3A4 enzyme was active. The results suggest that caution should be taken when interpreting the results of cell-based metabolism studies, and background metabolism may play a significant role in the data. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2018
- Full Text:
- Date Issued: 2018
Evaluating selected enterprises for sustainable social development in South Africa :|ba case study of Buffalo City Municipality, Eastern Cape
- Authors: Kajiita, Robert Mutemi
- Date: 2018
- Subjects: Social entrepreneurship Social responsibility of business Nonprofit organizations -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/10829 , vital:35825
- Description: Globally, countries, institutions, and organizations are investing time, resources, and human capital on finding best solutions to address poverty, inequality and unemployment; and importantly on achieving sustainable development. This study sought to investigate how social enterprises in Buffalo City Metropolitan Municipality (BCMM) contribute to sustainable social development within the municipality and the country at large. The study endeavoured to answer the following research questions: what is the knowledge level among the selected stakeholders on social enterprise in BCMM? What is the scope, application, and sustainability of selected social enterprises in BCMM? What is the contribution of selected social enterprises in social development in BCMM? What are the bottlenecks associated with the application of social entrepreneurship in BCMM? These questions were answered through mixed methods approaches and techniques, where both qualitative and quantitative data was collected and analysed. The data was collected through in-depth interviews (key informant technique) and a min-survey which was administered to users of the social enterprises. Qualitative data was analysed thematically, while quantitative data was statistically analysed with help of SPSS software. The interpretation of the findings was premised on pragmatic and transformative paradigms. The study further used theoretical lenses such as empowerment theory, capability approach and positive theory of social enterprise to support and explain the findings. The main findings showed that: the selected stakeholders conceptualized social enterprises through the African lens of communal living; the social enterprises widely provided their services across a range of sectors and geographical locations; the social enterprises had direct and indirect contribution to socio-economic development in BCMM. This was specifically through provision of employment, training and skill development among others; the future of social enterprises in the country is bright as many sectors presented opportunities for social investment; and social enterprises face financial constraints, lack of skills in the sector, and competition from the mainstream market. The recommendations from this study address government entities, social services providers and practitioners, social enterprises, and researchers on pertinent endeavours to improve the growth and development of social enterprises. The study concludes that social enterprises are: vital vehicles of socio-economic development, more especially for the marginalized segment of the populations; tools for reducing inequality; platforms for innovations, community participation and conduits of empowerment interventions. Thus, social enterprises play dynamic role and presents potential for capable sustainable social development in the local communities and the South African community at large.
- Full Text:
- Date Issued: 2018
- Authors: Kajiita, Robert Mutemi
- Date: 2018
- Subjects: Social entrepreneurship Social responsibility of business Nonprofit organizations -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/10829 , vital:35825
- Description: Globally, countries, institutions, and organizations are investing time, resources, and human capital on finding best solutions to address poverty, inequality and unemployment; and importantly on achieving sustainable development. This study sought to investigate how social enterprises in Buffalo City Metropolitan Municipality (BCMM) contribute to sustainable social development within the municipality and the country at large. The study endeavoured to answer the following research questions: what is the knowledge level among the selected stakeholders on social enterprise in BCMM? What is the scope, application, and sustainability of selected social enterprises in BCMM? What is the contribution of selected social enterprises in social development in BCMM? What are the bottlenecks associated with the application of social entrepreneurship in BCMM? These questions were answered through mixed methods approaches and techniques, where both qualitative and quantitative data was collected and analysed. The data was collected through in-depth interviews (key informant technique) and a min-survey which was administered to users of the social enterprises. Qualitative data was analysed thematically, while quantitative data was statistically analysed with help of SPSS software. The interpretation of the findings was premised on pragmatic and transformative paradigms. The study further used theoretical lenses such as empowerment theory, capability approach and positive theory of social enterprise to support and explain the findings. The main findings showed that: the selected stakeholders conceptualized social enterprises through the African lens of communal living; the social enterprises widely provided their services across a range of sectors and geographical locations; the social enterprises had direct and indirect contribution to socio-economic development in BCMM. This was specifically through provision of employment, training and skill development among others; the future of social enterprises in the country is bright as many sectors presented opportunities for social investment; and social enterprises face financial constraints, lack of skills in the sector, and competition from the mainstream market. The recommendations from this study address government entities, social services providers and practitioners, social enterprises, and researchers on pertinent endeavours to improve the growth and development of social enterprises. The study concludes that social enterprises are: vital vehicles of socio-economic development, more especially for the marginalized segment of the populations; tools for reducing inequality; platforms for innovations, community participation and conduits of empowerment interventions. Thus, social enterprises play dynamic role and presents potential for capable sustainable social development in the local communities and the South African community at large.
- Full Text:
- Date Issued: 2018
Evaluating the business improvement strategies, practices and challenges in the platinum mining industry
- Authors: Mulaudzi, Marubini David
- Date: 2018
- Subjects: Business planning -- South Africa , Decision making
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/32938 , vital:32412
- Description: The purpose of this study was to evaluate the strategies, practices and challenges relating to business performance and the implementation of business improvement to align production and maintenance strategies to the corporate strategy in Anglo American Platinum, a mining operation in South Africa. A set of questions were used in a qualitative research design through interview questions to collect data from corporate strategic management and the operational management involved in business improvement initiatives in the production and maintenance processes. Globally, mining companies are faced with many challenges, including safety, system integration and standardisation, caring for the environment, cost controlling and reliability of machinery and equipment. The mining industry is both labour and capital-intensive. While the South African labour force has unlimited demands on wages and salaries, most machinery runs at its maximum capacity and requires more capital for the operations to continue production, ensuring value creation for all stakeholders and hence sustainability of the operations. The situation was aggravated when market forces determining the commodity prices became volatile, causing the slump in the commodity prices. As a result, profit margins were eroded by the higher costs of production and higher wages costs. To create a sustainable, cost-effective operation and improve recovery and grades, mining companies such as Anglo American Platinum implemented a business improvement initiative. This study aims to evaluate the strategies, practices and challenges in relation to business improvement at Anglo American Platinum. Observations are that the impact of misalignment between corporate strategy, together with business improvement production and maintenance strategies, contribute minimally to the performance of production at Anglo American Platinum.
- Full Text:
- Date Issued: 2018
- Authors: Mulaudzi, Marubini David
- Date: 2018
- Subjects: Business planning -- South Africa , Decision making
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/32938 , vital:32412
- Description: The purpose of this study was to evaluate the strategies, practices and challenges relating to business performance and the implementation of business improvement to align production and maintenance strategies to the corporate strategy in Anglo American Platinum, a mining operation in South Africa. A set of questions were used in a qualitative research design through interview questions to collect data from corporate strategic management and the operational management involved in business improvement initiatives in the production and maintenance processes. Globally, mining companies are faced with many challenges, including safety, system integration and standardisation, caring for the environment, cost controlling and reliability of machinery and equipment. The mining industry is both labour and capital-intensive. While the South African labour force has unlimited demands on wages and salaries, most machinery runs at its maximum capacity and requires more capital for the operations to continue production, ensuring value creation for all stakeholders and hence sustainability of the operations. The situation was aggravated when market forces determining the commodity prices became volatile, causing the slump in the commodity prices. As a result, profit margins were eroded by the higher costs of production and higher wages costs. To create a sustainable, cost-effective operation and improve recovery and grades, mining companies such as Anglo American Platinum implemented a business improvement initiative. This study aims to evaluate the strategies, practices and challenges in relation to business improvement at Anglo American Platinum. Observations are that the impact of misalignment between corporate strategy, together with business improvement production and maintenance strategies, contribute minimally to the performance of production at Anglo American Platinum.
- Full Text:
- Date Issued: 2018
Evaluating the effects of catch-and-release angling on Cape stumpnose Rhabdosargus holubi in a South African estuary
- Arkert, N K, Childs, Amber-Robyn, Parkinson, Matthew C, Winkler, Alexander C, Butler, Edward C, Mannheim, Samantha L, Potts, Warren M
- Authors: Arkert, N K , Childs, Amber-Robyn , Parkinson, Matthew C , Winkler, Alexander C , Butler, Edward C , Mannheim, Samantha L , Potts, Warren M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124439 , vital:35613 , https://doi.10.2989/1814232x.2018.1494041
- Description: Fisheries managers are increasingly promoting catch and release (C&R) as a means to manage recreational fish stocks. Several commonly used output regulations, including bag and size limits, require the mandatory release of captured fishes by anglers. In addition to mandatory C&R, conservation-conscious anglers have adopted voluntary C&R behaviour as a result of the noticeable declines in the populations of many fishery species and this behaviour is becoming increasingly popular among recreational anglers (Cooke et al. 2013a). The combination of mandatory and voluntary C&R behaviour is substantial, as Raby et al. (2014) estimated that 60% of fish captured in global recreational fisheries are released. In South Africa, Cowley et al. (2013) found that a large proportion (mean 74% [SD 7.3]) of the five most-dominant fishery species captured in the Sundays Estuary recreational fishery were released.
- Full Text:
- Date Issued: 2018
- Authors: Arkert, N K , Childs, Amber-Robyn , Parkinson, Matthew C , Winkler, Alexander C , Butler, Edward C , Mannheim, Samantha L , Potts, Warren M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124439 , vital:35613 , https://doi.10.2989/1814232x.2018.1494041
- Description: Fisheries managers are increasingly promoting catch and release (C&R) as a means to manage recreational fish stocks. Several commonly used output regulations, including bag and size limits, require the mandatory release of captured fishes by anglers. In addition to mandatory C&R, conservation-conscious anglers have adopted voluntary C&R behaviour as a result of the noticeable declines in the populations of many fishery species and this behaviour is becoming increasingly popular among recreational anglers (Cooke et al. 2013a). The combination of mandatory and voluntary C&R behaviour is substantial, as Raby et al. (2014) estimated that 60% of fish captured in global recreational fisheries are released. In South Africa, Cowley et al. (2013) found that a large proportion (mean 74% [SD 7.3]) of the five most-dominant fishery species captured in the Sundays Estuary recreational fishery were released.
- Full Text:
- Date Issued: 2018
Evaluating the efficacy of Hypogeococcus sp. as a biological control agent of the cactaceous weed Cereus jamacaru in South Africa
- Sutton, Guy F, Klein, Hildegard, Paterson, Iain D
- Authors: Sutton, Guy F , Klein, Hildegard , Paterson, Iain D
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423350 , vital:72051 , xlink:href="https://doi.org/10.1007/s10526-018-9887-3"
- Description: We evaluated the efficacy of Hypogeococcus sp. (Hemiptera: Pseudococcidae) as a biological control agent of the cactaceous weed Cereus jamacaru De Candolle (Queen of the Night cactus) in South Africa. This weed has been described as being under complete biological control due to the action of Hypogeococcus sp., although no formal post-release evaluation had been conducted prior to this study. Biological control was associated with significant reductions in fruiting, plant survival and plant densities, while plant population age structures were negatively affected. Weed populations infected by Hypogeococcus sp. were typified by low or non-existent recruitment and are expected to diminish with time. Populations where Hypogeococcus sp. was absent displayed extensive recruitment, and are predicted to expand or self-replace, if left unchecked. These data indicate that Hypogeococcus sp. has a significant negative effect on C. jamacaru at the individual plant and population level, and given sufficient time provides complete biological control over this weed in South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Sutton, Guy F , Klein, Hildegard , Paterson, Iain D
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/423350 , vital:72051 , xlink:href="https://doi.org/10.1007/s10526-018-9887-3"
- Description: We evaluated the efficacy of Hypogeococcus sp. (Hemiptera: Pseudococcidae) as a biological control agent of the cactaceous weed Cereus jamacaru De Candolle (Queen of the Night cactus) in South Africa. This weed has been described as being under complete biological control due to the action of Hypogeococcus sp., although no formal post-release evaluation had been conducted prior to this study. Biological control was associated with significant reductions in fruiting, plant survival and plant densities, while plant population age structures were negatively affected. Weed populations infected by Hypogeococcus sp. were typified by low or non-existent recruitment and are expected to diminish with time. Populations where Hypogeococcus sp. was absent displayed extensive recruitment, and are predicted to expand or self-replace, if left unchecked. These data indicate that Hypogeococcus sp. has a significant negative effect on C. jamacaru at the individual plant and population level, and given sufficient time provides complete biological control over this weed in South Africa.
- Full Text:
- Date Issued: 2018
Evaluating the impact of energy policies on sustainable development in South Africa
- Authors: Julius, Ibitoye Oyebanji
- Date: 2018
- Subjects: Energy policy -- South Africa , Sustainable development -- South Africa Renewable energy sources -- South Africa Sustainable living -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30440 , vital:30944
- Description: The unachieved targets of the Millennium Development Goals (MDGs) at the end of 2015 by some developing countries including South Africa, lead to the evolution of the Sustainable Development Goals (SDGs). Ensuring access to sustainable, affordable, and modern energy is one of the 17 SDGs that the countries are expected to achieve before the end of 2030. Green growth is an important strategy for attaining this goal and a pathway to achieving the other goals. Therefore, an empirical study of the impact of energy policies on sustainable development is pertinent. Despite the importance of such research, there exists a gap in the literature relating to green growth and sustainable development from the SDGs point of view. This study fills the existing lacuna by developing an intertemporal holistic model which allows a study of the impact of energy policies on sustainable development in relation to the SDGs. Specifically, this thesis employs the Autoregressive Distributed Lag (ARDL) and Toda-Yamamoto approaches to analyse the long-run relationship and direction of causality respectively between green growth policies and sustainable development in South Africa from 1984 to 2016. This research provides insights into the importance of green growth for factors such as deforestation, fuel exports, energy imports and agricultural development, thus adding to the existing literature on the impact of renewable energy on a country’s social, environmental and economic conditions. Results from the analysis of the long-run relationship between green growth and macroeconomic variables, indicate a positive unidirectional relationship between changes in green growth policies and gross capital formation. This finding suggests that adopting green growth policies leads to increased investments. In contrast, green growth was found to have a negative effect on national income (gross domestic product). However, the Granger causality tests do not establish causality between these variables. Although green growth policies stimulate investments, high start-up costs associated with the implementation of these policies may mean that the resultant higher investments are yet to be translated to higher income levels in the South African economy. The findings indicate a boost in the South African balance of payment within the study period iv iv as evidenced by the positive long-run relationship between green growth policies and fuel exports. Additionally, the results indicate that green growth energy policies have assisted in improving the resilience to international oil price shocks as shown in the negative and significant long-run relationship between green growth and energy imports. With regard to environmental variables, results suggest a substitutability effect between green growth policies and the consumption of non-renewable energy. However, no evidence is found on the impact of green growth policies on the levels of deforestation and carbon dioxide(CO2) emissions, as well as natural resource depletion. In relation to the social variables, adoption of green growth policies was found to lead to improved educational quality, with causality running in both directions. This finding supports the feedback hypothesis. Similarly, green growth policies had a positive impact on agricultural development, again with causality established in both directions. The outcome of the long-run relationship between green energy consumption and unemployment shows that the green growth policies is yet to have a significant impact on unemployment reduction in South Africa. Green growth also has an insignificant and indirect relationship with GDP per capita; this result was affirmed by the absence of causality between GDP per capita and green energy consumption. This research not only makes contributions to the literature on the relevance of green growth policies for achieving economic, social and environmental sustainability, it also suggests possible policy implications which highlight the need for adoption and continued implementation of green growth policies in developing countries such as South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Julius, Ibitoye Oyebanji
- Date: 2018
- Subjects: Energy policy -- South Africa , Sustainable development -- South Africa Renewable energy sources -- South Africa Sustainable living -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30440 , vital:30944
- Description: The unachieved targets of the Millennium Development Goals (MDGs) at the end of 2015 by some developing countries including South Africa, lead to the evolution of the Sustainable Development Goals (SDGs). Ensuring access to sustainable, affordable, and modern energy is one of the 17 SDGs that the countries are expected to achieve before the end of 2030. Green growth is an important strategy for attaining this goal and a pathway to achieving the other goals. Therefore, an empirical study of the impact of energy policies on sustainable development is pertinent. Despite the importance of such research, there exists a gap in the literature relating to green growth and sustainable development from the SDGs point of view. This study fills the existing lacuna by developing an intertemporal holistic model which allows a study of the impact of energy policies on sustainable development in relation to the SDGs. Specifically, this thesis employs the Autoregressive Distributed Lag (ARDL) and Toda-Yamamoto approaches to analyse the long-run relationship and direction of causality respectively between green growth policies and sustainable development in South Africa from 1984 to 2016. This research provides insights into the importance of green growth for factors such as deforestation, fuel exports, energy imports and agricultural development, thus adding to the existing literature on the impact of renewable energy on a country’s social, environmental and economic conditions. Results from the analysis of the long-run relationship between green growth and macroeconomic variables, indicate a positive unidirectional relationship between changes in green growth policies and gross capital formation. This finding suggests that adopting green growth policies leads to increased investments. In contrast, green growth was found to have a negative effect on national income (gross domestic product). However, the Granger causality tests do not establish causality between these variables. Although green growth policies stimulate investments, high start-up costs associated with the implementation of these policies may mean that the resultant higher investments are yet to be translated to higher income levels in the South African economy. The findings indicate a boost in the South African balance of payment within the study period iv iv as evidenced by the positive long-run relationship between green growth policies and fuel exports. Additionally, the results indicate that green growth energy policies have assisted in improving the resilience to international oil price shocks as shown in the negative and significant long-run relationship between green growth and energy imports. With regard to environmental variables, results suggest a substitutability effect between green growth policies and the consumption of non-renewable energy. However, no evidence is found on the impact of green growth policies on the levels of deforestation and carbon dioxide(CO2) emissions, as well as natural resource depletion. In relation to the social variables, adoption of green growth policies was found to lead to improved educational quality, with causality running in both directions. This finding supports the feedback hypothesis. Similarly, green growth policies had a positive impact on agricultural development, again with causality established in both directions. The outcome of the long-run relationship between green energy consumption and unemployment shows that the green growth policies is yet to have a significant impact on unemployment reduction in South Africa. Green growth also has an insignificant and indirect relationship with GDP per capita; this result was affirmed by the absence of causality between GDP per capita and green energy consumption. This research not only makes contributions to the literature on the relevance of green growth policies for achieving economic, social and environmental sustainability, it also suggests possible policy implications which highlight the need for adoption and continued implementation of green growth policies in developing countries such as South Africa.
- Full Text:
- Date Issued: 2018
Evaluation and identification of microbial contaminants in polyherbal medicines used for the treatment of tuberculosis in Amathole District Municipality, Eastern Cape Province, South Africa
- Authors: Famewo, Elizabeth Bosede
- Date: 2018
- Subjects: Tuberculosis -- Treatment -- South Africa Mycobacterial diseases -- South Africa -- Eastern Cape Medicinal plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9680 , vital:34818
- Description: Tuberculosis is caused by Mycobacterium tuberculosis. The emergence of drug-resistant strains of this organism has become a global public health problem. In the continuing search for effective treatment, polyherbal medicines offer a great hope in the development of alternative drugs for the treatment of tuberculosis. The use of herbal formulations for therapeutic purposes has significantly increased in the developed and developing countries because of their curative property, less toxicity and minimal side effects. However, there is little information on their safety and effectiveness in the literature. To address this, polyherbal medicines used for the treatment of tuberculosis in the Eastern Cape Province of South Africa were evaluated. Ethno-medicinal survey was conducted through semi-structured questionnaires coupled with informal conversations with the herbal sellers in five communities in the study area. Bacterial and fungal DNA was extracted from the polyherbal medicines purchased. A fragment of the bacterial 16S rRNA gene and internal transcribed spacer region of the fungal rRNA operon were amplified with universal primers 27F and 518R, and ITS1 and ITS4 respectively. Following standard procedures, the amplicons were finally run on Illumina’s MiSeq platform. Furthermore, the remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. Agar dilution method was used to determine the minimum inhibitory concentration (MIC) of the remedies against eight bacteria and three fungi isolates. The herbal preparations were assayed for their toxicity using hatchability success and larval mortality of Artemia salina Leach. Finally, their nutritive properties were analysed using an inductively coupled plasma optical emission spectrometer for mineral analysis while the vitamins were determined using standardized methods A total of nine polyherbal preparations were collected. The herbs used for the preparation of these remedies belong to 20 families. Apiaceae [5(25 percent)] was the most prominent plant family used, followed by Liliaceae [4(20 percent)], Strychnaceae [4(20 percent)], Rutaceae [4(20 percent)] and Hypoxidaceae [3(15 percent)]. The two most frequently used plants were Allium sativum L. (Liliaceae) and Strychnos decussata (Pappe) Gilg. (Strychnaceae). Rhizomes was the most common parts used, followed by the roots and barks. The herbal medicines were prepared mainly by infusion and decoction. The presences of pathogenic and non-pathogenic bacteria were identified in the polyherbal medicines. Generally, the most common bacteria identified from the samples were Bacillus sp., Enterobacter sp., Klebsiella sp., Rahnella sp., Paenibacillus sp., Clostridium sp. and Pantoea sp. The predominant mycoflora obtained belongs to different genera or species of fungi; these include Alternaria, Candida, Ramularia, Cladosporium, Penicillium, Aspergillus and Malassezia. The susceptibility testing revealed that all the remedies contain anti-tubercular activity against M. tuberculosis H37Rv at concentrations below 50 ug/ml. Seven of the polyherbal preparations showed activity at concentrations below 25 ug/ml. The MIC values exhibited inhibitory activity at 1.562 μg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 μg/ml when compared to the polyherbal remedies. The inhibitory activity of the polyherbal medicines based on the overall MIC revealed that Hogsback first site (HBfs) and Fort Beaufort (FB) remedies were the most active remedies against the bacterial isolates at the concentration of 2.5 mg/mL. Among the nine herbal formulations, only King Williams Town site A (KWTa) remedy showed activity against Aspergillus niger and Aspergillus fumigatus with the MIC valve of 2.5 mg/mL. While King Williams Town site C (KWTc) and Hogsback third site (HBts) had the highest activity at 1.25 mg/mL against Candida albicans, the remaining remedies were active at 2.5 mg/mL. The percentage hatchability of 44.42 percent, 42.96 percent and 39.70 percent were observed in A. salina cysts incubated with herbal preparations from KWTa, HBfs and HBts respectively. The hatching success of the cysts in these remedies was significantly higher than the positive control (nystatin) and the negative control (sea water) at p < 0.05. The mortality of A. salina nauplii incubated in Alice (AL), King Williams Town site B (KWTb) and KWTc remedies were significantly higher than when larvae were incubated in both controls. Based on Meyer’s index, the LD50 of each polyherbal medicine was between 2.9 and 4.0 mg/ml, the LD50 values greater than 1 mg/ml, an indication that they are not toxic. The polyherbal preparations were found to be rich in vitamins and mineral nutrients. Calcium was the highest macronutrient detected while the lowest nutrient was phosphorus. Iron was the highest micronutrient in the majority of the polyherbal preparations while the lowest value was recorded for copper. Vitamin C was absent in the herbal preparations while vitamin A and E were detected. This study provides significant ethno-medicinal information on polyherbal medicines used for the treatment of TB in the study area. The presence of the identified bacteria and fungi in the herbal formulations is a cause for concern. However, the ability of the remedies to possess activity against Mycobacterium tuberculosis and other pathogenic microorganisms associated with tuberculosis infection makes them potential sources of new antimycobacterial agents. Also, they are rich sources of mineral nutrients, and are as well non-toxic, thus, they are safe for consumption. In view of their anti-tubercular properties, this study has provided a better understanding of the reasons why TB-patients make use of these polyherbal formulations. Also, the study supports the folkloric use of polyherbal medicines in the treatment of tuberculosis in the Eastern Cape Province, South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Famewo, Elizabeth Bosede
- Date: 2018
- Subjects: Tuberculosis -- Treatment -- South Africa Mycobacterial diseases -- South Africa -- Eastern Cape Medicinal plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9680 , vital:34818
- Description: Tuberculosis is caused by Mycobacterium tuberculosis. The emergence of drug-resistant strains of this organism has become a global public health problem. In the continuing search for effective treatment, polyherbal medicines offer a great hope in the development of alternative drugs for the treatment of tuberculosis. The use of herbal formulations for therapeutic purposes has significantly increased in the developed and developing countries because of their curative property, less toxicity and minimal side effects. However, there is little information on their safety and effectiveness in the literature. To address this, polyherbal medicines used for the treatment of tuberculosis in the Eastern Cape Province of South Africa were evaluated. Ethno-medicinal survey was conducted through semi-structured questionnaires coupled with informal conversations with the herbal sellers in five communities in the study area. Bacterial and fungal DNA was extracted from the polyherbal medicines purchased. A fragment of the bacterial 16S rRNA gene and internal transcribed spacer region of the fungal rRNA operon were amplified with universal primers 27F and 518R, and ITS1 and ITS4 respectively. Following standard procedures, the amplicons were finally run on Illumina’s MiSeq platform. Furthermore, the remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. Agar dilution method was used to determine the minimum inhibitory concentration (MIC) of the remedies against eight bacteria and three fungi isolates. The herbal preparations were assayed for their toxicity using hatchability success and larval mortality of Artemia salina Leach. Finally, their nutritive properties were analysed using an inductively coupled plasma optical emission spectrometer for mineral analysis while the vitamins were determined using standardized methods A total of nine polyherbal preparations were collected. The herbs used for the preparation of these remedies belong to 20 families. Apiaceae [5(25 percent)] was the most prominent plant family used, followed by Liliaceae [4(20 percent)], Strychnaceae [4(20 percent)], Rutaceae [4(20 percent)] and Hypoxidaceae [3(15 percent)]. The two most frequently used plants were Allium sativum L. (Liliaceae) and Strychnos decussata (Pappe) Gilg. (Strychnaceae). Rhizomes was the most common parts used, followed by the roots and barks. The herbal medicines were prepared mainly by infusion and decoction. The presences of pathogenic and non-pathogenic bacteria were identified in the polyherbal medicines. Generally, the most common bacteria identified from the samples were Bacillus sp., Enterobacter sp., Klebsiella sp., Rahnella sp., Paenibacillus sp., Clostridium sp. and Pantoea sp. The predominant mycoflora obtained belongs to different genera or species of fungi; these include Alternaria, Candida, Ramularia, Cladosporium, Penicillium, Aspergillus and Malassezia. The susceptibility testing revealed that all the remedies contain anti-tubercular activity against M. tuberculosis H37Rv at concentrations below 50 ug/ml. Seven of the polyherbal preparations showed activity at concentrations below 25 ug/ml. The MIC values exhibited inhibitory activity at 1.562 μg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 μg/ml when compared to the polyherbal remedies. The inhibitory activity of the polyherbal medicines based on the overall MIC revealed that Hogsback first site (HBfs) and Fort Beaufort (FB) remedies were the most active remedies against the bacterial isolates at the concentration of 2.5 mg/mL. Among the nine herbal formulations, only King Williams Town site A (KWTa) remedy showed activity against Aspergillus niger and Aspergillus fumigatus with the MIC valve of 2.5 mg/mL. While King Williams Town site C (KWTc) and Hogsback third site (HBts) had the highest activity at 1.25 mg/mL against Candida albicans, the remaining remedies were active at 2.5 mg/mL. The percentage hatchability of 44.42 percent, 42.96 percent and 39.70 percent were observed in A. salina cysts incubated with herbal preparations from KWTa, HBfs and HBts respectively. The hatching success of the cysts in these remedies was significantly higher than the positive control (nystatin) and the negative control (sea water) at p < 0.05. The mortality of A. salina nauplii incubated in Alice (AL), King Williams Town site B (KWTb) and KWTc remedies were significantly higher than when larvae were incubated in both controls. Based on Meyer’s index, the LD50 of each polyherbal medicine was between 2.9 and 4.0 mg/ml, the LD50 values greater than 1 mg/ml, an indication that they are not toxic. The polyherbal preparations were found to be rich in vitamins and mineral nutrients. Calcium was the highest macronutrient detected while the lowest nutrient was phosphorus. Iron was the highest micronutrient in the majority of the polyherbal preparations while the lowest value was recorded for copper. Vitamin C was absent in the herbal preparations while vitamin A and E were detected. This study provides significant ethno-medicinal information on polyherbal medicines used for the treatment of TB in the study area. The presence of the identified bacteria and fungi in the herbal formulations is a cause for concern. However, the ability of the remedies to possess activity against Mycobacterium tuberculosis and other pathogenic microorganisms associated with tuberculosis infection makes them potential sources of new antimycobacterial agents. Also, they are rich sources of mineral nutrients, and are as well non-toxic, thus, they are safe for consumption. In view of their anti-tubercular properties, this study has provided a better understanding of the reasons why TB-patients make use of these polyherbal formulations. Also, the study supports the folkloric use of polyherbal medicines in the treatment of tuberculosis in the Eastern Cape Province, South Africa.
- Full Text:
- Date Issued: 2018
Evaluation of pharmacological properties and anti-neoplastic potentials of pelargonium iquinans and opuntia stricta extracts using acute leukemia cell lines
- Authors: Izuegbuna, Ogochukwu Osita
- Date: 2018
- Subjects: Pelargoniums Medicinal plants
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/10353/10372 , vital:35442
- Description: Leukaemia like other cancers is a public health issue, and coupled with the issue of drug resistance, toxicity and refractory disease novel treatments both as definitive and supportive care become necessary. The dissertation was designed to evaluate two medicinal plants (Opuntia stricta and Pelargonium inquinans) and their antineoplastic potentials. The phytochemicals analyses were done using standard methods with three different solvents (acetone, aqueous and ethanol). Vitamins analyses was also done using standard methods. The result revealed that vitamin A,C and E were present in the plants. Cell based anti-inflammatory activity were carried out using RAW264.7 macrophages cell, and COX-2 and NO expression as inflammatory markers. Cell based cytotoxicity and antineoplastic activity was investigated using RAW 264.7 macrophages cell, U937 and Jurkat cells. The results of the phytochemical analyses using GC/MS showed that phenols, flavonoids, flavonol, tannin, proanthocyanidin, saponin, alkaloids and phytate were present in these plants. In vitro antioxidant activity was carried out using nitric oxide (NO), alpha, alpha-diphenyl- β-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), phosphomolybdenum activity, and iron reducing power (FRAP) techniques. The results of the antioxidant activity showed both plants had antioxidant properties that can be attributed to the phytochemical and vitamins contents. The results showed that both plants had anti-inflammatory properties which can also be attributed to their phytochemicals. The GC-MS analysis of the essential oils of both plants showed the presence of monoterpenes, sesquiterpenes, fatty acids, and esters. The results showed that O. stricta extracts have mild cytotoxicity, while P. inquinans have strong cytotoxicity. The cell cycle analysis showed Pelargonium inquinans extracts induced G0/G1 phase arrest and cleaved caspase-3. This study has shown the antineoplastic potential of P. inquinans which may be attributed to some phytochemicals in it such as carophyllene, humulene, beta myrcene and beta pinene.
- Full Text:
- Date Issued: 2018
- Authors: Izuegbuna, Ogochukwu Osita
- Date: 2018
- Subjects: Pelargoniums Medicinal plants
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/10353/10372 , vital:35442
- Description: Leukaemia like other cancers is a public health issue, and coupled with the issue of drug resistance, toxicity and refractory disease novel treatments both as definitive and supportive care become necessary. The dissertation was designed to evaluate two medicinal plants (Opuntia stricta and Pelargonium inquinans) and their antineoplastic potentials. The phytochemicals analyses were done using standard methods with three different solvents (acetone, aqueous and ethanol). Vitamins analyses was also done using standard methods. The result revealed that vitamin A,C and E were present in the plants. Cell based anti-inflammatory activity were carried out using RAW264.7 macrophages cell, and COX-2 and NO expression as inflammatory markers. Cell based cytotoxicity and antineoplastic activity was investigated using RAW 264.7 macrophages cell, U937 and Jurkat cells. The results of the phytochemical analyses using GC/MS showed that phenols, flavonoids, flavonol, tannin, proanthocyanidin, saponin, alkaloids and phytate were present in these plants. In vitro antioxidant activity was carried out using nitric oxide (NO), alpha, alpha-diphenyl- β-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), phosphomolybdenum activity, and iron reducing power (FRAP) techniques. The results of the antioxidant activity showed both plants had antioxidant properties that can be attributed to the phytochemical and vitamins contents. The results showed that both plants had anti-inflammatory properties which can also be attributed to their phytochemicals. The GC-MS analysis of the essential oils of both plants showed the presence of monoterpenes, sesquiterpenes, fatty acids, and esters. The results showed that O. stricta extracts have mild cytotoxicity, while P. inquinans have strong cytotoxicity. The cell cycle analysis showed Pelargonium inquinans extracts induced G0/G1 phase arrest and cleaved caspase-3. This study has shown the antineoplastic potential of P. inquinans which may be attributed to some phytochemicals in it such as carophyllene, humulene, beta myrcene and beta pinene.
- Full Text:
- Date Issued: 2018