Investigation into factors affecting water provision in Eastern Cape Municipalities, with specific focus on OR Tambo district Municipality
- Authors: Santi, Sifiso Kanyo
- Date: 2018
- Subjects: Water-supply, Rural -- South Africa -- Eastern Cape , Water-supply -- South Africa -- Eastern Cape Water resources development -- Economic aspects Sustainable development -- Economic aspects
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10948/34810 , vital:33454
- Description: The provision of basic water services to all South African communities is one of the biggest challenges that many local municipalities are currently facing. The objective of this study was to investigate factors affecting water provision in Eastern Cape municipalities, with specific focus on OR Tambo District Municipality. As a Water Service Authority it has the responsibility to ensure that all communities at the district have access to basic water supply. The study evaluated the participant’s views who are municipal officials responsible in water services delivery. The study shows that there is a serious challenge of backlogs in water delivery and these will be difficult to overcome due to financial limitation, lack of personnel capacity. It also revealed that some of the areas in the district are not serviced due to the aging of infrastructure, Data collection was done by using the interview methods which involved direct interactions with the participants from the Water and Sanitation department at the OR Tambo District Municipality who provided answers to the questions of the researcher. The study recommends that the municipality should prioritise looking at other integrated water cycle management strategies that will ease the delivery of water to the citizens. Awareness campaigns for efficient and effective use of water should be launched. Water supply and management must be one of the areas that the municipality prioritise in terms of budgeting on establishing programs to prevent water losses in the system. Maintenance and effective use of the already available resources must be effected at the municipality.
- Full Text:
- Date Issued: 2018
- Authors: Santi, Sifiso Kanyo
- Date: 2018
- Subjects: Water-supply, Rural -- South Africa -- Eastern Cape , Water-supply -- South Africa -- Eastern Cape Water resources development -- Economic aspects Sustainable development -- Economic aspects
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10948/34810 , vital:33454
- Description: The provision of basic water services to all South African communities is one of the biggest challenges that many local municipalities are currently facing. The objective of this study was to investigate factors affecting water provision in Eastern Cape municipalities, with specific focus on OR Tambo District Municipality. As a Water Service Authority it has the responsibility to ensure that all communities at the district have access to basic water supply. The study evaluated the participant’s views who are municipal officials responsible in water services delivery. The study shows that there is a serious challenge of backlogs in water delivery and these will be difficult to overcome due to financial limitation, lack of personnel capacity. It also revealed that some of the areas in the district are not serviced due to the aging of infrastructure, Data collection was done by using the interview methods which involved direct interactions with the participants from the Water and Sanitation department at the OR Tambo District Municipality who provided answers to the questions of the researcher. The study recommends that the municipality should prioritise looking at other integrated water cycle management strategies that will ease the delivery of water to the citizens. Awareness campaigns for efficient and effective use of water should be launched. Water supply and management must be one of the areas that the municipality prioritise in terms of budgeting on establishing programs to prevent water losses in the system. Maintenance and effective use of the already available resources must be effected at the municipality.
- Full Text:
- Date Issued: 2018
Investigation of molecular mechanism of recognition between citral and MARK4: A newer therapeutic approach to attenuate cancer cell progression
- Naz, Farha, Khan, Faez I, Mohammad, Taj, Khan, Parvez, Manzoor, Saaliqa, Hasan, Gulam M, Lobb, Kevin A, Luqman, Suaib, Ahmad, Faizan, Hassan, M Imtaiyaz
- Authors: Naz, Farha , Khan, Faez I , Mohammad, Taj , Khan, Parvez , Manzoor, Saaliqa , Hasan, Gulam M , Lobb, Kevin A , Luqman, Suaib , Ahmad, Faizan , Hassan, M Imtaiyaz
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/447967 , vital:74687 , xlink:href="https://doi.org/10.1016/j.ijbiomac.2017.10.143"
- Description: Microtubule affinity regulating kinase 4 (MARK4) is a member of AMP-activated protein kinase, found to be involved in apoptosis, inflammation and many other regulatory pathways. Since, its aberrant expression is directly associated with the cell cycle and thus cancer. Therefore, MARK4 is being considered as a potential drug target for cancer therapy. Here, we investigated the mechanism of inhibition of MARK4 activity by citral. Docking studies suggested that citral effectively binds to the active site cavity, and complex is stabilized by several interactions. We further performed molecular dynamics simulation of MARK4-citral complex under explicit water condition for 100 ns and observed that binding of citral to MARK4 was quite stable. Fluorescence binding studies suggested that citral strongly binds to MARK4 and thereby inhibits its enzyme activity which was measured by the kinase inhibition assay. We further performed MTT assay and observed that citral inhibits proliferation of breast cancer cell line MCF-7. This work provides a newer insight into the use of citral as novel cancer therapeutics through the MARK4 inhibition. Results may be employed to design novel therapeutic molecule using citral as a scaffold for MARK4 inhibition to fight related diseases.
- Full Text:
- Date Issued: 2018
- Authors: Naz, Farha , Khan, Faez I , Mohammad, Taj , Khan, Parvez , Manzoor, Saaliqa , Hasan, Gulam M , Lobb, Kevin A , Luqman, Suaib , Ahmad, Faizan , Hassan, M Imtaiyaz
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/447967 , vital:74687 , xlink:href="https://doi.org/10.1016/j.ijbiomac.2017.10.143"
- Description: Microtubule affinity regulating kinase 4 (MARK4) is a member of AMP-activated protein kinase, found to be involved in apoptosis, inflammation and many other regulatory pathways. Since, its aberrant expression is directly associated with the cell cycle and thus cancer. Therefore, MARK4 is being considered as a potential drug target for cancer therapy. Here, we investigated the mechanism of inhibition of MARK4 activity by citral. Docking studies suggested that citral effectively binds to the active site cavity, and complex is stabilized by several interactions. We further performed molecular dynamics simulation of MARK4-citral complex under explicit water condition for 100 ns and observed that binding of citral to MARK4 was quite stable. Fluorescence binding studies suggested that citral strongly binds to MARK4 and thereby inhibits its enzyme activity which was measured by the kinase inhibition assay. We further performed MTT assay and observed that citral inhibits proliferation of breast cancer cell line MCF-7. This work provides a newer insight into the use of citral as novel cancer therapeutics through the MARK4 inhibition. Results may be employed to design novel therapeutic molecule using citral as a scaffold for MARK4 inhibition to fight related diseases.
- Full Text:
- Date Issued: 2018
Investigation of strategies employed in rural households to improve food security in Raymond Mhlaba Local Municipality : a case of Zihlahleni Location in Middledrift, Eastern Cape Province
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
- Date Issued: 2018
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
- Date Issued: 2018
Investigation of the potential separation of isomers and related compounds using host compound (2R,3R)-(−)-2,3-dimethoxy-1,1,4,4-tetraphenylbutane-1,4-diol
- Authors: Pohl, Pieter Lourens
- Date: 2018
- Subjects: Chemistry, Organic , Clathrate compounds Thermal analysis
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23131 , vital:30432
- Description: In this study, we investigated the potential of a host compound, (2R,3R)-(−)-2,3-dimethoxy-1,1,4,4-tetraphenylbutane-1,4-diol (DMT), for use in the separation of isomers and related compounds using host-guest chemistry. The title molecule, DMT, is composed of a butane chain bearing hydroxyl moieties on the terminal carbons and methoxy moieties on the two internal, chiral carbon atoms. In addition, there are two phenyl rings on each of the terminal carbons. The synthesis of DMT was carried out by subjecting the diester of naturally-occurring optically active tartaric acid to a Grignard reaction employing phenylmagnesium bromide. Subsequent methylation of the secondary hydroxy groups with dimethyl sulfate afforded DMT. The resulting host molecule was investigated for its inclusion abilities by crystallizing with a number of potential aromatic, aliphatic and alicyclic guests such as toluene, aniline, nitrobenzene, anisole, cyclohexane, ethyl acetate and ethanol. Host:guest ratios were determined by means of 1H-NMR spectroscopy. Of the hosts investigated, DMT favoured a host:guest ratio of 2:1 for all included guests investigated. It complexed with most non-polycyclic aromatic guests as well as cyclohexane, cyclohexene and cyclohexanone. It was not able to include short chain or branched alcohols such as methanol, ethanol or 2-propanol, or other hetero-aliphatic or hetero- cyclic compounds such as diethyl ether, acetonitrile, morpholine or dioxane. Competition inclusion experiments were performed in which DMT was crystallized from equimolar and non-equimolar binary, ternary and quaternary mixtures of appropriate guests. The mother liquor mixtures and resultant crystals were subjected to GC-MS analysis in order to determine whether DMT showed discriminatory behaviour towards the guests from a mixture. It was observed that DMT was able to differentiate between related compounds, for example, the host preferred to include N,N-dimethylaniline compared with N-methylaniline and aniline. The host also discriminated against isomers, for example, p-xylene was preferentially included over o-xylene and m-xylene, while o-cresol was included in preference to p-cresol and m-cresol. Single crystal X-ray analysis was used to investigate the host–guest interactions responsible for guest inclusion, as well as to discern reasons for the host’s selective behaviour. X-ray data for the inclusion complexes indicated that each complex was isostructural, crystallizing in the monoclinic C2 crystal system. A pair of 1,3- and 2,4- intramolecular hydrogen bonds, as well as intramolecular non-classic hydrogen bonds between adjacent ortho-aromatic hydrogens and hydroxy moieties was a significant stabilizing factor for the geometry of the host. Guests were held within discrete cavities in the crystal lattice, and experienced only π–π stacking, CH–π interactions and other short contacts. Thermal analyses were used to determine the relative thermal stabilities of the complexes, and these data compared to the selectivity preference of DMT, obtained from the competition experiments, in order to assess the reasons for any discriminatory behaviour. Finally, Hirshfeld surface analysis data was used to determine if the thermal stability of the complexes was related to the number and type of interactions between host and guest.
- Full Text:
- Date Issued: 2018
- Authors: Pohl, Pieter Lourens
- Date: 2018
- Subjects: Chemistry, Organic , Clathrate compounds Thermal analysis
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23131 , vital:30432
- Description: In this study, we investigated the potential of a host compound, (2R,3R)-(−)-2,3-dimethoxy-1,1,4,4-tetraphenylbutane-1,4-diol (DMT), for use in the separation of isomers and related compounds using host-guest chemistry. The title molecule, DMT, is composed of a butane chain bearing hydroxyl moieties on the terminal carbons and methoxy moieties on the two internal, chiral carbon atoms. In addition, there are two phenyl rings on each of the terminal carbons. The synthesis of DMT was carried out by subjecting the diester of naturally-occurring optically active tartaric acid to a Grignard reaction employing phenylmagnesium bromide. Subsequent methylation of the secondary hydroxy groups with dimethyl sulfate afforded DMT. The resulting host molecule was investigated for its inclusion abilities by crystallizing with a number of potential aromatic, aliphatic and alicyclic guests such as toluene, aniline, nitrobenzene, anisole, cyclohexane, ethyl acetate and ethanol. Host:guest ratios were determined by means of 1H-NMR spectroscopy. Of the hosts investigated, DMT favoured a host:guest ratio of 2:1 for all included guests investigated. It complexed with most non-polycyclic aromatic guests as well as cyclohexane, cyclohexene and cyclohexanone. It was not able to include short chain or branched alcohols such as methanol, ethanol or 2-propanol, or other hetero-aliphatic or hetero- cyclic compounds such as diethyl ether, acetonitrile, morpholine or dioxane. Competition inclusion experiments were performed in which DMT was crystallized from equimolar and non-equimolar binary, ternary and quaternary mixtures of appropriate guests. The mother liquor mixtures and resultant crystals were subjected to GC-MS analysis in order to determine whether DMT showed discriminatory behaviour towards the guests from a mixture. It was observed that DMT was able to differentiate between related compounds, for example, the host preferred to include N,N-dimethylaniline compared with N-methylaniline and aniline. The host also discriminated against isomers, for example, p-xylene was preferentially included over o-xylene and m-xylene, while o-cresol was included in preference to p-cresol and m-cresol. Single crystal X-ray analysis was used to investigate the host–guest interactions responsible for guest inclusion, as well as to discern reasons for the host’s selective behaviour. X-ray data for the inclusion complexes indicated that each complex was isostructural, crystallizing in the monoclinic C2 crystal system. A pair of 1,3- and 2,4- intramolecular hydrogen bonds, as well as intramolecular non-classic hydrogen bonds between adjacent ortho-aromatic hydrogens and hydroxy moieties was a significant stabilizing factor for the geometry of the host. Guests were held within discrete cavities in the crystal lattice, and experienced only π–π stacking, CH–π interactions and other short contacts. Thermal analyses were used to determine the relative thermal stabilities of the complexes, and these data compared to the selectivity preference of DMT, obtained from the competition experiments, in order to assess the reasons for any discriminatory behaviour. Finally, Hirshfeld surface analysis data was used to determine if the thermal stability of the complexes was related to the number and type of interactions between host and guest.
- Full Text:
- Date Issued: 2018
Investigation of the subsurface geology using time domain, magnetic and electrical resistivity geophysical techniques in the Karoo basin at Beaufort west, Western Cape Province, South Africa
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
- Date Issued: 2018
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
- Date Issued: 2018
Investigation of the thermo-chemical behaviour of coal-algae agglomerates
- Authors: Baloyi, Hope
- Date: 2018
- Subjects: Biomass energy , Coal -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23913 , vital:30642
- Description: There is a growing research interest in the co-processing of biomass and coal, with the aim of addressing the negative attributes associated with the thermal processing of coal alone. Biomass feedstocks are regarded as a clean, renewable source, and the co-utilization of biomass feedstocks with coal is deemed to have a potential to reduce emission of pollutants (i.e. NOx and SOx) and volatile organic compounds (VOC’s). Moreover, biomass are thermally reactive and thus facilitate the conversion of coal during co-processing. Biomass material and coal are two autonomous fuel materials with different chemical characteristics and have a dissimilar thermal behaviour making it difficult to achieve chemical interaction between the two solid fuels to contribute to the formation of products. Coalgae® Technology developed at the Nelson Mandela University, involves the biological treatment of coal fines by adsorbing live microalgae biomass (in slurry form) onto waste coal fines to form coal-microalgae agglomerates. This new innovative approach seeks to integrate bio-based feedstock into coal thermal processing and to improve the utilization and thermal efficiency of coal fines as well as the interaction between the volatile components of biomass and coal during thermal processing (e.g. devolatilization), thereby overcoming some of the challenges that confront the co-processing of coal and biomass. Coal fines are low-ranked coals, generally characterized by high contents of sulphur, high ash yields, low calorific values and poor thermal reactivity, and these attributes limits the thermo-chemical processing of the coal fines. Therefore, this investigation was undertaken to assess the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorbing live microalgae slurry at varying ratios onto coal fines. For this purpose, the effects of adsorbing microalgae at varying ratios on the chemical characteristics and thermal behaviour of coal fines under pyrolytic conditions were investigated. The primary aim was to assess whether the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorption of live microalgae onto fine coal, is substantively modified compared to a simple additive model of the original coal and pre-dried microalgae biomass samples. Results obtained from the proximate analyses performed on an Eltra Thermo-gravimetric analyzer (TGA) thermostep, have shown that the adsorption of microalgae slurry onto coal fines does not possess greater influence in improving the yield of volatiles and ash in coal fines than can be expected from a simple additive model of the original raw materials. Based on the ultimate analyses results, it was found that the adsorption of microalgae slurry resulted in a systematic reduction in the sulphur content, a notable increase in the hydrogen and oxygen contents, however, no significant disparities were found between the measured ultimate properties of coal-microalgae agglomerates as compared to the theoretically-expected ultimate properties from a simple linear combination of parental coal and microalgae biomass. Assessment of the thermal behaviour of parental samples and coal-microalgae agglomerates involved the use non-isothermal (40-900ºC, 20 K/min) thermogravimetry under inert conditions. It was found that the adsorption of microalgae slurry onto coal fines resulted in an improved thermal reactivity of coal fines, although, did not affect the overall pyrolysis characteristics of the coal fines. Comparison of the thermal profiles (measured and calculated TG/DTG curves), revealed that the yield of volatile products during the pyrolysis of coal-microalgae blends do not exceed the expected volatile yields from a simple combination of coal and microalgae biomass. These results suggest that there was no positive or accelerative synergistic interaction between volatile components of adsorbed microalgae and coal fines during pyrolysis. Mild pyrolysis of raw coal and coal-microalgae performed in a fixed-bed reactor furnace (450ºC), resulted in improved yields of Fossil-Bio crude (FBC) oil (derived from coal-microalgae pyrolysis), at increased biomass ratio compared to coal tar. FBC Oil was found to contain relatively high contents of oxygen, hydrogen, and low sulphur content than coal tar. GC-MS analyses showed the presence of a heterocyclic compounds (i.e. Indole and 2, 6 dimethyl pyridine) in the FBC oil and these were not identified in the coal tar. Furthermore, high boiling compounds such as Flourene, pyrene and pentacosane were identified in the coal tar, however not identified in the FBC oil. Simulated distillation results showed notable differences between the FBC oil and coal tar in terms of the distribution of boiling point fractions particularly, high boing point components. Semi-devolatilized chars derived from coal-microalgae agglomerates showed substantial degree of decarboxylation and dehydrogenation compared to the coal chars.
- Full Text:
- Date Issued: 2018
- Authors: Baloyi, Hope
- Date: 2018
- Subjects: Biomass energy , Coal -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/23913 , vital:30642
- Description: There is a growing research interest in the co-processing of biomass and coal, with the aim of addressing the negative attributes associated with the thermal processing of coal alone. Biomass feedstocks are regarded as a clean, renewable source, and the co-utilization of biomass feedstocks with coal is deemed to have a potential to reduce emission of pollutants (i.e. NOx and SOx) and volatile organic compounds (VOC’s). Moreover, biomass are thermally reactive and thus facilitate the conversion of coal during co-processing. Biomass material and coal are two autonomous fuel materials with different chemical characteristics and have a dissimilar thermal behaviour making it difficult to achieve chemical interaction between the two solid fuels to contribute to the formation of products. Coalgae® Technology developed at the Nelson Mandela University, involves the biological treatment of coal fines by adsorbing live microalgae biomass (in slurry form) onto waste coal fines to form coal-microalgae agglomerates. This new innovative approach seeks to integrate bio-based feedstock into coal thermal processing and to improve the utilization and thermal efficiency of coal fines as well as the interaction between the volatile components of biomass and coal during thermal processing (e.g. devolatilization), thereby overcoming some of the challenges that confront the co-processing of coal and biomass. Coal fines are low-ranked coals, generally characterized by high contents of sulphur, high ash yields, low calorific values and poor thermal reactivity, and these attributes limits the thermo-chemical processing of the coal fines. Therefore, this investigation was undertaken to assess the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorbing live microalgae slurry at varying ratios onto coal fines. For this purpose, the effects of adsorbing microalgae at varying ratios on the chemical characteristics and thermal behaviour of coal fines under pyrolytic conditions were investigated. The primary aim was to assess whether the thermo-chemical behaviour of coal-microalgae agglomerates, formed by adsorption of live microalgae onto fine coal, is substantively modified compared to a simple additive model of the original coal and pre-dried microalgae biomass samples. Results obtained from the proximate analyses performed on an Eltra Thermo-gravimetric analyzer (TGA) thermostep, have shown that the adsorption of microalgae slurry onto coal fines does not possess greater influence in improving the yield of volatiles and ash in coal fines than can be expected from a simple additive model of the original raw materials. Based on the ultimate analyses results, it was found that the adsorption of microalgae slurry resulted in a systematic reduction in the sulphur content, a notable increase in the hydrogen and oxygen contents, however, no significant disparities were found between the measured ultimate properties of coal-microalgae agglomerates as compared to the theoretically-expected ultimate properties from a simple linear combination of parental coal and microalgae biomass. Assessment of the thermal behaviour of parental samples and coal-microalgae agglomerates involved the use non-isothermal (40-900ºC, 20 K/min) thermogravimetry under inert conditions. It was found that the adsorption of microalgae slurry onto coal fines resulted in an improved thermal reactivity of coal fines, although, did not affect the overall pyrolysis characteristics of the coal fines. Comparison of the thermal profiles (measured and calculated TG/DTG curves), revealed that the yield of volatile products during the pyrolysis of coal-microalgae blends do not exceed the expected volatile yields from a simple combination of coal and microalgae biomass. These results suggest that there was no positive or accelerative synergistic interaction between volatile components of adsorbed microalgae and coal fines during pyrolysis. Mild pyrolysis of raw coal and coal-microalgae performed in a fixed-bed reactor furnace (450ºC), resulted in improved yields of Fossil-Bio crude (FBC) oil (derived from coal-microalgae pyrolysis), at increased biomass ratio compared to coal tar. FBC Oil was found to contain relatively high contents of oxygen, hydrogen, and low sulphur content than coal tar. GC-MS analyses showed the presence of a heterocyclic compounds (i.e. Indole and 2, 6 dimethyl pyridine) in the FBC oil and these were not identified in the coal tar. Furthermore, high boiling compounds such as Flourene, pyrene and pentacosane were identified in the coal tar, however not identified in the FBC oil. Simulated distillation results showed notable differences between the FBC oil and coal tar in terms of the distribution of boiling point fractions particularly, high boing point components. Semi-devolatilized chars derived from coal-microalgae agglomerates showed substantial degree of decarboxylation and dehydrogenation compared to the coal chars.
- Full Text:
- Date Issued: 2018
Iron (iii) porphyrin electrocatalyzed enantioselective carbon-chloride bond cleavage of hexachlorocyclohexanes (HCHs): combined experimental investigation and theoretical calculations
- Liang, Xu, Li, Minzhi, Mack, John, Lobb, Kevin A, Zhu, Weihua
- Authors: Liang, Xu , Li, Minzhi , Mack, John , Lobb, Kevin A , Zhu, Weihua
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/447979 , vital:74688 , xlink:href="https://pubs.rsc.org/en/content/articlehtml/2018/dt/c8dt02510j"
- Description: Enantioselective electrocatalysis of α-, β-, γ- and δ-hexachlorocyclohexanes (HCHs) by tetrakis-pentafluorophenyl-Fe(III)porphyrin is described. The first example of the combined use of electrochemical measurements and theoretical calculations to determine the mechanism of the enantioselective C–Cl bond cleavage of the electrocatalysis is reported. The electrochemical measurements demonstrate that the reactivity of the HCHs follows the order γ-HCH > α-HCH > δ-HCH > β-HCH. Steric considerations and a molecular orbital theory approach can be used to rationalize the enantioselective nature of the catalysis based on the ease of approach of each Cl atom to the central Fe(I) ion and a consideration of the nodes on the C–Cl bonds that weaken these bonds in a manner that results in bond cleavage and the formation of an Fe–Cl bond.
- Full Text:
- Date Issued: 2018
- Authors: Liang, Xu , Li, Minzhi , Mack, John , Lobb, Kevin A , Zhu, Weihua
- Date: 2018
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/447979 , vital:74688 , xlink:href="https://pubs.rsc.org/en/content/articlehtml/2018/dt/c8dt02510j"
- Description: Enantioselective electrocatalysis of α-, β-, γ- and δ-hexachlorocyclohexanes (HCHs) by tetrakis-pentafluorophenyl-Fe(III)porphyrin is described. The first example of the combined use of electrochemical measurements and theoretical calculations to determine the mechanism of the enantioselective C–Cl bond cleavage of the electrocatalysis is reported. The electrochemical measurements demonstrate that the reactivity of the HCHs follows the order γ-HCH > α-HCH > δ-HCH > β-HCH. Steric considerations and a molecular orbital theory approach can be used to rationalize the enantioselective nature of the catalysis based on the ease of approach of each Cl atom to the central Fe(I) ion and a consideration of the nodes on the C–Cl bonds that weaken these bonds in a manner that results in bond cleavage and the formation of an Fe–Cl bond.
- Full Text:
- Date Issued: 2018
Is craft brewing a threat to the sales of mainstream brands in South Africa
- Authors: Martin, Carmen Estelle
- Date: 2018
- Subjects: Sales management -- South Africa , Brand name products -- South Africa Marketing -- South Africa -- Management Brewing -- Economic aspects Beer -- South Africa -- History
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22563 , vital:30006
- Description: The study aims to answer the question of whether craft brewing is a threat to the sales of mainstream brands in South Africa. This is done through a quantitative multiple case research study into craft breweries within South Africa. The justification of the research resides within the hype around the increase in craft breweries within South Africa. The theoretical framework of the thesis consists of history of beer, the elements of beer, the supply of raw materials to manufacture beer and the definition of craft beer. The comparison is done between the United States Craft beer market and the South African Beer market. The empirical part of the study is divided into six parts. It starts with an explanation of the method of the study, the questionnaire design as well as the data collection methods. Thereafter the topic is further justified, and validity, reliability, limitations and ethical issues will be considered. The results and analysis and findings section there are 212 craft brewers appearing on the Brewers Association membership list, the purposive sampling method meant that the questionnaires were sent out to 192 craft brewers across South Africa. Of the total of 192 questionnaires distributed, only 22 responded to the study, effectively granting us a usable response rate of 11.5%. Due to the closed niche of the alcohol market that craft brewing is competing in, as well as the low participation rate, it was concluded that within South Africa craft brewing though a contender does not impact mainstream brands.
- Full Text:
- Date Issued: 2018
- Authors: Martin, Carmen Estelle
- Date: 2018
- Subjects: Sales management -- South Africa , Brand name products -- South Africa Marketing -- South Africa -- Management Brewing -- Economic aspects Beer -- South Africa -- History
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22563 , vital:30006
- Description: The study aims to answer the question of whether craft brewing is a threat to the sales of mainstream brands in South Africa. This is done through a quantitative multiple case research study into craft breweries within South Africa. The justification of the research resides within the hype around the increase in craft breweries within South Africa. The theoretical framework of the thesis consists of history of beer, the elements of beer, the supply of raw materials to manufacture beer and the definition of craft beer. The comparison is done between the United States Craft beer market and the South African Beer market. The empirical part of the study is divided into six parts. It starts with an explanation of the method of the study, the questionnaire design as well as the data collection methods. Thereafter the topic is further justified, and validity, reliability, limitations and ethical issues will be considered. The results and analysis and findings section there are 212 craft brewers appearing on the Brewers Association membership list, the purposive sampling method meant that the questionnaires were sent out to 192 craft brewers across South Africa. Of the total of 192 questionnaires distributed, only 22 responded to the study, effectively granting us a usable response rate of 11.5%. Due to the closed niche of the alcohol market that craft brewing is competing in, as well as the low participation rate, it was concluded that within South Africa craft brewing though a contender does not impact mainstream brands.
- Full Text:
- Date Issued: 2018
Isolating the consumer determinants of choice when making a purchasing decision in a supplier company
- Authors: Williams, Rita Christine
- Date: 2018
- Subjects: Customer relations , Customer services -- Management Consumer satisfaction -- Evaluation
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/36048 , vital:33885
- Description: The objective of this research was to establish the determinants of purchase choice by customers and to establish the modality of their decision-making process. The study was conducted at PerkinElmer a scientific equipment supplier company. PerkinElmer South Africa supplies equipment to industrial laboratories, academic laboratories and laboratories in government institutions. It has become clear to the researcher that understanding the customer and what their perceptions are is the key to business success. These insights would give the researcher the knowledge of important factors in the customer modality of purchase decisions. The literature review revealed that there are numerous factors that influences a customer buying decision namely: Customer relationships management, Unique selling proposition, the importance of salespeople, customer satisfaction levels, the brand name, Brand awareness, brand perception, brand loyalty, price, the service levels that the company provides after sale. The customer decision-making process and the various stages involved, an empirical study was conducted utilising in-depth interviews. The five main themes that were investigated through in-depth interviews were the following: Customer relationship management, brand, customer satisfaction levels, price, service. The factors were probed, analysed and compared and each customer’s preference was noted. The study employs a qualitative approach as this stance supports the researcher’s interest in the customer views and perceptions. A small sample was utilised from the scientific sector in the Eastern Cape and the Gauteng area. The empirical results indicated the importance of service support and if this is not in place it can mean the loss of future business. The customers in academia had the common view that the price is most important. The university policy forces the lowest priced equipment to be purchased. The respondents unanimously voiced their concerns regarding the expensiveness of PerkinElmer consumables.
- Full Text:
- Date Issued: 2018
- Authors: Williams, Rita Christine
- Date: 2018
- Subjects: Customer relations , Customer services -- Management Consumer satisfaction -- Evaluation
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/36048 , vital:33885
- Description: The objective of this research was to establish the determinants of purchase choice by customers and to establish the modality of their decision-making process. The study was conducted at PerkinElmer a scientific equipment supplier company. PerkinElmer South Africa supplies equipment to industrial laboratories, academic laboratories and laboratories in government institutions. It has become clear to the researcher that understanding the customer and what their perceptions are is the key to business success. These insights would give the researcher the knowledge of important factors in the customer modality of purchase decisions. The literature review revealed that there are numerous factors that influences a customer buying decision namely: Customer relationships management, Unique selling proposition, the importance of salespeople, customer satisfaction levels, the brand name, Brand awareness, brand perception, brand loyalty, price, the service levels that the company provides after sale. The customer decision-making process and the various stages involved, an empirical study was conducted utilising in-depth interviews. The five main themes that were investigated through in-depth interviews were the following: Customer relationship management, brand, customer satisfaction levels, price, service. The factors were probed, analysed and compared and each customer’s preference was noted. The study employs a qualitative approach as this stance supports the researcher’s interest in the customer views and perceptions. A small sample was utilised from the scientific sector in the Eastern Cape and the Gauteng area. The empirical results indicated the importance of service support and if this is not in place it can mean the loss of future business. The customers in academia had the common view that the price is most important. The university policy forces the lowest priced equipment to be purchased. The respondents unanimously voiced their concerns regarding the expensiveness of PerkinElmer consumables.
- Full Text:
- Date Issued: 2018
Isolation and Characterisation of Stigmasterol and β–Sitosterol from Anthocleista djalonensis A. Chev.
- Okoro, Ijeoma S, Tor-Anyiin, Terrumun A, Igoli, John O, Noundou, Xavier S, Krause, Rui W M
- Authors: Okoro, Ijeoma S , Tor-Anyiin, Terrumun A , Igoli, John O , Noundou, Xavier S , Krause, Rui W M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126191 , vital:35857 , https://doi.org/10.9734/AJOCS/2017/37147
- Description: Aim: Anthocleista djalonensis A. Chev. is a plant with several chemical constituents whichaccounts for its ethno-pharmacological uses. The present study is aimed at identifying and characterizing the active principles from the roots of the plant. Place and Duration of Study: The study was carried out at the Department of Organic Chemistry,Rhodes University, Grahamstown, South Africa between March and July 2016. Methodology: The root powder was subjected to maceration with methanol to obtain the crude extract. The methanol extract was fractionated using hexane, ethyl acetate and acetone successively. The acetone extract was thereafter subjected to column chromatography to isolate any pure components. Results: White needle-like crystals were obtained which on spectral analysis(IR, 1H NMR, 13C NMR, 2D- NMR, and ESI-MS ) were identified as a mixture of stigmasterol and β-sitosterol. Conclusion: The compounds isolated were identified as stigmasterol and β-sitosterol.
- Full Text:
- Date Issued: 2018
- Authors: Okoro, Ijeoma S , Tor-Anyiin, Terrumun A , Igoli, John O , Noundou, Xavier S , Krause, Rui W M
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126191 , vital:35857 , https://doi.org/10.9734/AJOCS/2017/37147
- Description: Aim: Anthocleista djalonensis A. Chev. is a plant with several chemical constituents whichaccounts for its ethno-pharmacological uses. The present study is aimed at identifying and characterizing the active principles from the roots of the plant. Place and Duration of Study: The study was carried out at the Department of Organic Chemistry,Rhodes University, Grahamstown, South Africa between March and July 2016. Methodology: The root powder was subjected to maceration with methanol to obtain the crude extract. The methanol extract was fractionated using hexane, ethyl acetate and acetone successively. The acetone extract was thereafter subjected to column chromatography to isolate any pure components. Results: White needle-like crystals were obtained which on spectral analysis(IR, 1H NMR, 13C NMR, 2D- NMR, and ESI-MS ) were identified as a mixture of stigmasterol and β-sitosterol. Conclusion: The compounds isolated were identified as stigmasterol and β-sitosterol.
- Full Text:
- Date Issued: 2018
Isolation and characterization of E. coli and Campylobacter spp. from diarrhoeal samples collected from selected hospitals in Amathole District Municipality, Eastern Cape, South Africa
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
- Date Issued: 2018
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
- Date Issued: 2018
Isolation, identification and genetic characterisation of a microsporidium isolated from the carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
- Authors: Lloyd, Melissa
- Date: 2018
- Subjects: Pyralidae , Pyralidae -- Genetics , Pyralidae -- Phylogeny , Pyralidae -- Pathogens , Cladistic analysis , Transmission electron microscopy , Carob moth (Ectomyelois ceratoniae)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61894 , vital:28075
- Description: Carob moth, Ectomyelois ceratoniae (Zeller) (Lepidoptera: Pyralidae) is an economically important pest, yet its biology and pest status on citrus in South Africa was, until recently, poorly understood. A study was initiated to determine the cause of collapse of a laboratory carob moth colony that was established to investigate the biology of carob moth on citrus and to develop integrated management strategies for the pest. An organism was isolated from deceased larvae and was morphologically identified as a microsporidium, based on transmission electron microscopy. Microsporidia are obligate intracellular parasites that have been found to infect almost all eukaryotes. Several Nosema species have been isolated from economically important insect pests, yet little genetic information is available from online databases for identification. Mature spores were recovered and measured using transmission electron microscopy. Spores were ovocylindrical with a wrinkled exospore, and had a length of 2.8 ± 0.02 pm and a width of 1.6 ± 0.04 pm. The identity of the microsporidium was confirmed by PCR amplification, sequencing and analysis of the regions encoding the ribosomal RNA. BLAST analysis of the different rRNA regions amplified showed that the microsporidium shared a 96 - 99 % identity with Nosema sp. M-Pr, Nosema carpocapsae, Nosema oulemae, Nosema sp. CO1, Microsporidium 57864, and Nosema bombi. Phylogenetic analysis of the SSU and LSU rRNA genes showed that the microsporidium clustered with the Nosema / Vairimorpha clade, supported by a bootstrap value of 100. The organisation of the RNA cistron was determined by PCR amplification using the primer set 18f and L1328r to be 5’-SSU-ITS-LSU-IGS-5S-3’, which confirms the placement of the microsporidium within the Nosema / Vairimorpha clade. Because the BLAST results showed a close relationship with Nosema carpocapsae, a microsporidium infecting codling moth, the pathogenicity of the microsporidium was tested against codling moth by inoculating artificial diet with a high spore concentration of 1.1 x 107 spores/ml and a low spore concentration of 1.1 x 104 spores/ml. DNA was extracted from deceased larvae inoculated with the high concentration, and PCR of the SSU rRNA gene and bacterial 16S region was performed. Mortality in the high concentration experiment was significant (p = 0.05), but the cause of infection was determined to be a bacterium, through sequencing and BLAST analysis of the bacterial 16S rDNA. The bacterium shared a 99 % identity with Bacillus cereus. Percentage mortality (p = 0.09), larval mass (p = 0.09) and instar (p = 0.24) did not differ significantly between treatments in the low concentration experiment. DNA was extracted from the larvae and PCR amplification of the SSU rRNA gene was performed to determine whether microsporidia were present. No SSU bands were observed in any of the treatments and percentage mortality was not significant, thus it was determined that no infection occurred. This is the first study to report the genetic characterisation of a microsporidium isolated from carob moth and provides important genetic information for classification of microsporidia within the Nosema / Vairimorpha clade. It is also one of few studies in which the complete rRNA cistron of a species within the Nosema / Vairimorpha clade has been sequenced. The identification of a microsporidium from a laboratory colony of carob moth is important as it provides information about pathogens infecting the carob moth and constraints to carob moth rearing, which is useful for further studies on rearing carob moth and for establishment of a clean colony for research purposes.
- Full Text:
- Date Issued: 2018
- Authors: Lloyd, Melissa
- Date: 2018
- Subjects: Pyralidae , Pyralidae -- Genetics , Pyralidae -- Phylogeny , Pyralidae -- Pathogens , Cladistic analysis , Transmission electron microscopy , Carob moth (Ectomyelois ceratoniae)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/61894 , vital:28075
- Description: Carob moth, Ectomyelois ceratoniae (Zeller) (Lepidoptera: Pyralidae) is an economically important pest, yet its biology and pest status on citrus in South Africa was, until recently, poorly understood. A study was initiated to determine the cause of collapse of a laboratory carob moth colony that was established to investigate the biology of carob moth on citrus and to develop integrated management strategies for the pest. An organism was isolated from deceased larvae and was morphologically identified as a microsporidium, based on transmission electron microscopy. Microsporidia are obligate intracellular parasites that have been found to infect almost all eukaryotes. Several Nosema species have been isolated from economically important insect pests, yet little genetic information is available from online databases for identification. Mature spores were recovered and measured using transmission electron microscopy. Spores were ovocylindrical with a wrinkled exospore, and had a length of 2.8 ± 0.02 pm and a width of 1.6 ± 0.04 pm. The identity of the microsporidium was confirmed by PCR amplification, sequencing and analysis of the regions encoding the ribosomal RNA. BLAST analysis of the different rRNA regions amplified showed that the microsporidium shared a 96 - 99 % identity with Nosema sp. M-Pr, Nosema carpocapsae, Nosema oulemae, Nosema sp. CO1, Microsporidium 57864, and Nosema bombi. Phylogenetic analysis of the SSU and LSU rRNA genes showed that the microsporidium clustered with the Nosema / Vairimorpha clade, supported by a bootstrap value of 100. The organisation of the RNA cistron was determined by PCR amplification using the primer set 18f and L1328r to be 5’-SSU-ITS-LSU-IGS-5S-3’, which confirms the placement of the microsporidium within the Nosema / Vairimorpha clade. Because the BLAST results showed a close relationship with Nosema carpocapsae, a microsporidium infecting codling moth, the pathogenicity of the microsporidium was tested against codling moth by inoculating artificial diet with a high spore concentration of 1.1 x 107 spores/ml and a low spore concentration of 1.1 x 104 spores/ml. DNA was extracted from deceased larvae inoculated with the high concentration, and PCR of the SSU rRNA gene and bacterial 16S region was performed. Mortality in the high concentration experiment was significant (p = 0.05), but the cause of infection was determined to be a bacterium, through sequencing and BLAST analysis of the bacterial 16S rDNA. The bacterium shared a 99 % identity with Bacillus cereus. Percentage mortality (p = 0.09), larval mass (p = 0.09) and instar (p = 0.24) did not differ significantly between treatments in the low concentration experiment. DNA was extracted from the larvae and PCR amplification of the SSU rRNA gene was performed to determine whether microsporidia were present. No SSU bands were observed in any of the treatments and percentage mortality was not significant, thus it was determined that no infection occurred. This is the first study to report the genetic characterisation of a microsporidium isolated from carob moth and provides important genetic information for classification of microsporidia within the Nosema / Vairimorpha clade. It is also one of few studies in which the complete rRNA cistron of a species within the Nosema / Vairimorpha clade has been sequenced. The identification of a microsporidium from a laboratory colony of carob moth is important as it provides information about pathogens infecting the carob moth and constraints to carob moth rearing, which is useful for further studies on rearing carob moth and for establishment of a clean colony for research purposes.
- Full Text:
- Date Issued: 2018
Jesus, Che, Luaty: on the relationship between a digital picture and an iconic image in political iconography in Angola
- Authors: Siegert, Nadine
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145919 , vital:38478 , DOI: 10.2979/africatoday.65.1.04
- Description: This essay makes a close examination of a selection of images of the Angolan rapper Luaty Beirão, who became internationally known as a political activist during his imprisonment in June 2015, accused of staging a coup d'état. By analyzing and interpreting images that were highly mediatized during that period, this article shows how political iconography can be traced back to Christian iconography and other images. Such filiations of images and their mediatization invoke a power that contributes to the formation of political and popular icons. This article analyzes this nexus by deconstructing the transmutation of a photograph into a popular icon.
- Full Text:
- Date Issued: 2018
- Authors: Siegert, Nadine
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/145919 , vital:38478 , DOI: 10.2979/africatoday.65.1.04
- Description: This essay makes a close examination of a selection of images of the Angolan rapper Luaty Beirão, who became internationally known as a political activist during his imprisonment in June 2015, accused of staging a coup d'état. By analyzing and interpreting images that were highly mediatized during that period, this article shows how political iconography can be traced back to Christian iconography and other images. Such filiations of images and their mediatization invoke a power that contributes to the formation of political and popular icons. This article analyzes this nexus by deconstructing the transmutation of a photograph into a popular icon.
- Full Text:
- Date Issued: 2018
Job embeddedness, employee retention and innovative work behaviours at a selected municipality
- Authors: Lippert, Cuan Roberto
- Date: 2018
- Subjects: Employee retention -- South Africa , Organizational behavior -- South Africa Work ethic -- South Africa Work -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22520 , vital:29996
- Description: The importance of job embeddedness with its combined forces of fit, links and sacrifice has previously been centred on predicting retention. At the present time, job embeddedness is also considered as a factor influencing innovative work behaviours. The current study extends this line of research by examining the relationship between job embeddedness, employee retention and innovative work behaviours of employees working in the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. Research on how job embeddedness influences employee retention and innovative work behaviours, is important in the light of retaining knowledgeable staff in the current municipal setting. A cross-sectional quantitative survey was conducted on a non-probability purposive sample (N = 131) in the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. Correlational results revealed a significant positive linear relationship between on-the-job embeddedness and innovative work behaviours. However, a significant negative linear relationship was reported between on-the-job embeddedness and retention, measured by employees’ perceptions of job prospects and future intentions to leave the organisation. Furthermore, a significant positive linear relationship was reported between off-the-job embeddedness and innovative work behaviours. The study revealed significant differences between age and on-the-job embeddedness. Lastly, significant differences were found between marital status, language and tenure in relation to off-the-job embeddedness. The findings of the study contribute to the research body of knowledge with respect to employee retention and innovative work behaviours at the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. The final chapter concludes the research with recommendations for future research practices.
- Full Text:
- Date Issued: 2018
- Authors: Lippert, Cuan Roberto
- Date: 2018
- Subjects: Employee retention -- South Africa , Organizational behavior -- South Africa Work ethic -- South Africa Work -- Psychological aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/22520 , vital:29996
- Description: The importance of job embeddedness with its combined forces of fit, links and sacrifice has previously been centred on predicting retention. At the present time, job embeddedness is also considered as a factor influencing innovative work behaviours. The current study extends this line of research by examining the relationship between job embeddedness, employee retention and innovative work behaviours of employees working in the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. Research on how job embeddedness influences employee retention and innovative work behaviours, is important in the light of retaining knowledgeable staff in the current municipal setting. A cross-sectional quantitative survey was conducted on a non-probability purposive sample (N = 131) in the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. Correlational results revealed a significant positive linear relationship between on-the-job embeddedness and innovative work behaviours. However, a significant negative linear relationship was reported between on-the-job embeddedness and retention, measured by employees’ perceptions of job prospects and future intentions to leave the organisation. Furthermore, a significant positive linear relationship was reported between off-the-job embeddedness and innovative work behaviours. The study revealed significant differences between age and on-the-job embeddedness. Lastly, significant differences were found between marital status, language and tenure in relation to off-the-job embeddedness. The findings of the study contribute to the research body of knowledge with respect to employee retention and innovative work behaviours at the Nelson Mandela Bay Municipality Infrastructure and Engineering Directorate. The final chapter concludes the research with recommendations for future research practices.
- Full Text:
- Date Issued: 2018
Job satisfaction of community pharmacists in Port Elizabeth
- Authors: Barendse, Denver
- Date: 2018
- Subjects: Job satisfaction -- South Africa -- Port Elizabeth , Pharmacy -- Practice -- South Africa -- Port Elizabeth Pharmacists -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/23880 , vital:30638
- Description: Job satisfaction and motivation play an important role in job retention and increased productivity of pharmacists which in turn improves pharmaceutical care performance. Pharmacies in Port Elizabeth have an increased pharmacist shortage and many community or retail pharmacies must overcome challenges to retain pharmacists. The study was quantitative in nature. A descriptive and exploratory approach was used. The research objective was to conduct a questionnaire survey of pharmacists working in community pharmacies in Port Elizabeth to assess the levels of job satisfaction and report the findings with recommendations to management. A modified Job Descriptive Index questionnaire was used to determine the level of job satisfaction. The variables used to determine the level of satisfaction was job content, monetary remuneration, promotion, co-workers, and supervision. Retention strategies was used to determine effective strategies managers can use to retain pharmacists. Positive and negative aspects of community pharmacy was also determined. A total of 48 pharmacies were approached by the researcher, 27 questionnaires were distributed to eligible participants which met the inclusion criteria and 23 questionnaires was returned, thus giving a response rate of 85%. It was observed that job satisfaction was positive in areas of job experience or content, customer interaction, interpersonal relationship with co-workers and supervision. The areas of job dissatisfaction were monetary remuneration and promotional opportunities. The retention strategies which received the most effective score were competitive financial packages (87%) and personal development (86%). The means of the retention strategies were high and even closer to 5 which indicated the “most effective” score. The pharmacists were not satisfied with monetary remuneration, promotion, recognition and performance appraisal provided by the organisations. The pharmacists are feeling over burden towards the workload. Management in this regard should pay attention to findings.
- Full Text:
- Date Issued: 2018
- Authors: Barendse, Denver
- Date: 2018
- Subjects: Job satisfaction -- South Africa -- Port Elizabeth , Pharmacy -- Practice -- South Africa -- Port Elizabeth Pharmacists -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/23880 , vital:30638
- Description: Job satisfaction and motivation play an important role in job retention and increased productivity of pharmacists which in turn improves pharmaceutical care performance. Pharmacies in Port Elizabeth have an increased pharmacist shortage and many community or retail pharmacies must overcome challenges to retain pharmacists. The study was quantitative in nature. A descriptive and exploratory approach was used. The research objective was to conduct a questionnaire survey of pharmacists working in community pharmacies in Port Elizabeth to assess the levels of job satisfaction and report the findings with recommendations to management. A modified Job Descriptive Index questionnaire was used to determine the level of job satisfaction. The variables used to determine the level of satisfaction was job content, monetary remuneration, promotion, co-workers, and supervision. Retention strategies was used to determine effective strategies managers can use to retain pharmacists. Positive and negative aspects of community pharmacy was also determined. A total of 48 pharmacies were approached by the researcher, 27 questionnaires were distributed to eligible participants which met the inclusion criteria and 23 questionnaires was returned, thus giving a response rate of 85%. It was observed that job satisfaction was positive in areas of job experience or content, customer interaction, interpersonal relationship with co-workers and supervision. The areas of job dissatisfaction were monetary remuneration and promotional opportunities. The retention strategies which received the most effective score were competitive financial packages (87%) and personal development (86%). The means of the retention strategies were high and even closer to 5 which indicated the “most effective” score. The pharmacists were not satisfied with monetary remuneration, promotion, recognition and performance appraisal provided by the organisations. The pharmacists are feeling over burden towards the workload. Management in this regard should pay attention to findings.
- Full Text:
- Date Issued: 2018
Judging psycholegal reporting in civil court proceedings in the Eastern Cape
- Authors: Marais, Claire Anne
- Date: 2018
- Subjects: Court proceedings -- South Africa -- Eastern Cape , Evidence, Expert -- South Africa -- Eastern Cape , Forensic psychology -- South Africa -- Eastern Cape , Quality Rating Scale (QRS) , Psycholegal reporting -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/62999 , vital:28352
- Description: Purpose: The primary purpose for this research was to evaluate the quality of psycholegal reports, and the qualification of the authors. Previous research had revealed generalised poor practices in psycholegal report writing and the authors seemed unqualified as expert witness. This negatively influenced the impression and usefulness of expert psychological opinion in South African courts. The secondary purpose was to compare the results to those determined by Ireland (2012), which was a useful benchmark study in the UK as her findings corresponded with the literature. Method: This archival research analysed a hand-search sample of 20 reports written by 20 psychologists based nationally. These reports were submitted as expert opinion in civil proceedings in the Grahamstown Division of the Eastern Cape High Court between 2011 and 2016. Ireland and Pinschof’s (2009) measure ‘Quality Rating Scale’ was adapted for a South African context and used to evaluate quantitatively the reports. Focus was on the reports’ contents (fact and opinion), methods, process and overall rating, and the qualification of the authors. Results: Overall, two-thirds of the reports were rated as ‘good’ and ‘very good’. The results were generally more positive than Ireland’s (2012) findings. However, there were practices in the report writing that were deemed detrimental to the quality of the expert’s opinion. This included vague referral questions and unclear basis for concluding opinions and diagnosis. There was also a lack of discussions surrounding the scientific trustworthiness of testing methods and absent ethical considerations. Lastly, the specialised expertise of the experts was not adequately documented. Conclusion: The findings painted a more positive picture of psycholegal reporting in South Africa than illustrated in previous research, which seemingly relied on anecdotes and subjective surveys. This study used a systematic and objective measure. The findings of this study, previous research and ethical Rules of Conduct were used to consider recommendations for psycholegal report writing, and the improvement of the field of psycholegal work. Lastly, the focus of future research was discussed.
- Full Text:
- Date Issued: 2018
- Authors: Marais, Claire Anne
- Date: 2018
- Subjects: Court proceedings -- South Africa -- Eastern Cape , Evidence, Expert -- South Africa -- Eastern Cape , Forensic psychology -- South Africa -- Eastern Cape , Quality Rating Scale (QRS) , Psycholegal reporting -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/62999 , vital:28352
- Description: Purpose: The primary purpose for this research was to evaluate the quality of psycholegal reports, and the qualification of the authors. Previous research had revealed generalised poor practices in psycholegal report writing and the authors seemed unqualified as expert witness. This negatively influenced the impression and usefulness of expert psychological opinion in South African courts. The secondary purpose was to compare the results to those determined by Ireland (2012), which was a useful benchmark study in the UK as her findings corresponded with the literature. Method: This archival research analysed a hand-search sample of 20 reports written by 20 psychologists based nationally. These reports were submitted as expert opinion in civil proceedings in the Grahamstown Division of the Eastern Cape High Court between 2011 and 2016. Ireland and Pinschof’s (2009) measure ‘Quality Rating Scale’ was adapted for a South African context and used to evaluate quantitatively the reports. Focus was on the reports’ contents (fact and opinion), methods, process and overall rating, and the qualification of the authors. Results: Overall, two-thirds of the reports were rated as ‘good’ and ‘very good’. The results were generally more positive than Ireland’s (2012) findings. However, there were practices in the report writing that were deemed detrimental to the quality of the expert’s opinion. This included vague referral questions and unclear basis for concluding opinions and diagnosis. There was also a lack of discussions surrounding the scientific trustworthiness of testing methods and absent ethical considerations. Lastly, the specialised expertise of the experts was not adequately documented. Conclusion: The findings painted a more positive picture of psycholegal reporting in South Africa than illustrated in previous research, which seemingly relied on anecdotes and subjective surveys. This study used a systematic and objective measure. The findings of this study, previous research and ethical Rules of Conduct were used to consider recommendations for psycholegal report writing, and the improvement of the field of psycholegal work. Lastly, the focus of future research was discussed.
- Full Text:
- Date Issued: 2018
Key considerations for novel aptamer generation and aptasensor platform design: a case study on human α-thrombin and histamine as sensor targets
- Authors: Ho, Lance St John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63534 , vital:28432
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
- Authors: Ho, Lance St John
- Date: 2018
- Subjects: Uncatalogued
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63534 , vital:28432
- Description: Expected release date-April 2020
- Full Text:
- Date Issued: 2018
Knowledge and knowers in Educational Leadership and Management (ELM) Master’s Programmes in South Africa
- Authors: Kajee, Farhana Amod
- Date: 2018
- Subjects: Educational leadership -- South Africa , Teachers -- Training of -- South Africa , Education, Higher -- South Africa , Master of education degree -- South Africa , Knowledge, Theory of , Educational sociology -- South Africa , Education, Higher -- Aims and objectives -- South Africa , Legitimation Code Theory (LCT)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60698 , vital:27819
- Description: This dissertation examines the knowledge and knower practices in the Master’s in Educational Leadership and Management (ELM) coursework programmes at South African public universities. This study was prompted by my growing awareness of problems and tensions in the field of ELM generally, and at the level of programme design of the M Ed degree in particular. Many of these had been identified by a national audit of coursework M Eds in ELM (CHE, 2010), and this study sought to find a way of theorising these with a view to improving both course design and teaching. To this end I employed Maton’s Legitimation Code Theory (LCT) which enables critical engagement with knowledge and knowers in programmes, how they are positioned, and how this positioning may be problematic. Hence my first research question sought to discover and critique what counted as knowledge in these programmes and why, while the second asked how knowers were positioned, and why this had come to be the case. LCT has its roots in the work of Bernstein and Maton, whose preoccupation with curriculum was/is driven by a sense of social justice: if we can understand how and why the curriculum is organised and presented in a particular way, it becomes possible to re-imagine teaching and learning, making it accessible to a broader, more inclusive body of learners. The study also drew on critical realism as an underlabourer. This philosophy provided a nuanced understanding of ontology, encouraging and enabling me, as researcher, to unearth causal mechanisms driving the status quo. Only seven South African universities currently offer the coursework option of a Master’s degree in ELM, compared to thirteen when the audit was conducted in 2010. Six of the universities agreed to take part in the study. Data was gathered through content analysis of the six course outlines and interviews with individual co-ordinators or academics centrally involved in the programmes. Through the development of a translation device I was able to establishing that a knower code was dominant in the programmes. Using this point as my departure, I interrogated the knowledge practices and found that different types of knowledge were being privileged across the programmes, with some having a practical/professional leaning and others a more academic/theoretical orientation. The resultant tension does, I argue, restrict knowledge building and helps to account for the fact that the field is generally considered to be under-theorised. The fact all of these programme are registered with the same national qualifications authority, ostensibly following the same national guidelines for Master’s degrees is worrying. The study attempts to find underlying, historically significant reasons for this unevenness. An analysis of the programmes revealed a leaning towards supportive pedagogical approaches. While all programmes promote a cultivated gaze their purposes are not always the same. While a hegemonic practices potential for opening counts as knowledge, cultivated gaze can enable transformation, it can also encourage that can impede real change and empowerment. The study has the up much needed debate on what is meant by a Master’s in ELM, what and what kinds of knower are envisaged.
- Full Text:
- Date Issued: 2018
- Authors: Kajee, Farhana Amod
- Date: 2018
- Subjects: Educational leadership -- South Africa , Teachers -- Training of -- South Africa , Education, Higher -- South Africa , Master of education degree -- South Africa , Knowledge, Theory of , Educational sociology -- South Africa , Education, Higher -- Aims and objectives -- South Africa , Legitimation Code Theory (LCT)
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/60698 , vital:27819
- Description: This dissertation examines the knowledge and knower practices in the Master’s in Educational Leadership and Management (ELM) coursework programmes at South African public universities. This study was prompted by my growing awareness of problems and tensions in the field of ELM generally, and at the level of programme design of the M Ed degree in particular. Many of these had been identified by a national audit of coursework M Eds in ELM (CHE, 2010), and this study sought to find a way of theorising these with a view to improving both course design and teaching. To this end I employed Maton’s Legitimation Code Theory (LCT) which enables critical engagement with knowledge and knowers in programmes, how they are positioned, and how this positioning may be problematic. Hence my first research question sought to discover and critique what counted as knowledge in these programmes and why, while the second asked how knowers were positioned, and why this had come to be the case. LCT has its roots in the work of Bernstein and Maton, whose preoccupation with curriculum was/is driven by a sense of social justice: if we can understand how and why the curriculum is organised and presented in a particular way, it becomes possible to re-imagine teaching and learning, making it accessible to a broader, more inclusive body of learners. The study also drew on critical realism as an underlabourer. This philosophy provided a nuanced understanding of ontology, encouraging and enabling me, as researcher, to unearth causal mechanisms driving the status quo. Only seven South African universities currently offer the coursework option of a Master’s degree in ELM, compared to thirteen when the audit was conducted in 2010. Six of the universities agreed to take part in the study. Data was gathered through content analysis of the six course outlines and interviews with individual co-ordinators or academics centrally involved in the programmes. Through the development of a translation device I was able to establishing that a knower code was dominant in the programmes. Using this point as my departure, I interrogated the knowledge practices and found that different types of knowledge were being privileged across the programmes, with some having a practical/professional leaning and others a more academic/theoretical orientation. The resultant tension does, I argue, restrict knowledge building and helps to account for the fact that the field is generally considered to be under-theorised. The fact all of these programme are registered with the same national qualifications authority, ostensibly following the same national guidelines for Master’s degrees is worrying. The study attempts to find underlying, historically significant reasons for this unevenness. An analysis of the programmes revealed a leaning towards supportive pedagogical approaches. While all programmes promote a cultivated gaze their purposes are not always the same. While a hegemonic practices potential for opening counts as knowledge, cultivated gaze can enable transformation, it can also encourage that can impede real change and empowerment. The study has the up much needed debate on what is meant by a Master’s in ELM, what and what kinds of knower are envisaged.
- Full Text:
- Date Issued: 2018
Knowledge and practices of primary health care professional nurses: screening and identification of intimate partner violence
- Authors: Felix, Rehanna Theresa
- Date: 2018
- Subjects: Marital violence -- South Africa -- Psychological aspects , Nursing -- Social aspects Primary health care -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MCur
- Identifier: http://hdl.handle.net/10948/29816 , vital:30781
- Description: Intimate partner violence is a global concern that affects most countries. In South Africa women are mostly affected by intimate partner violence, which often results in physical injuries or mental disorders. The World Health Organization compiled guidelines to combat the problem of intimate partner violence against women with emphasis on screening for intimate partner violence. However, a screening tool to identify intimate partner violence in female patients was absent in the Nelson Mandela Bay Health District, resulting in cases of intimate partner violence being unidentified. The aim of the research study is to determine the knowledge and practices of professional nurses regarding intimate partner violence and to introduce the use of an intimate partner violence screening tool in primary healthcare facilities to assist professional nurses to identify intimate partner violence. The objectives were firstly, to determine the current knowledge and practices utilized by professional nurses to identify intimate partner violence; secondly, to introduce an intimate partner violence screening tool to identify intimate partner violence; thirdly, to evaluate the knowledge of the professional nurses obtained in the educational session and practices post-implementation of the intimate partner violence screening tool. The researcher conducted a small intervention research study using quantitative, quasi-experimental one group pre-and post-test design. An all-inclusive sample consisting of professional nurses working in primary healthcare facilities in Nelson Mandela Bay Health District was included in the study. The research study was conducted in three phases: Phase one, the pre-test determined the current knowledge and practices of the professional nurses to identify intimate partner violence. Phase two, entailed educational sessions to educate professional nurses that participated in Phase one regarding intimate partner violence and the intimate partner violence screening tool. Phase three, focused on the post-test to evaluate the knowledge of the professional nurses obtained in the educational session and practices post-implementation of the intimate partner violence screening tool. The data was analysed through descriptive and inferential statistics with assistance of the statistician using frequency distribution, central tendency, Chi-square, t-test, Cohens’d and Cramér’s V and Cronbach’s alpha. The researchers ensured reliability and validity throughout the study. The researcher applied the ethical principles of beneficence, non-maleficence iii and autonomy, as well as privacy and confidentiality. Ethical permission was obtained for the study. The response in the pre-test was (n1=128) and the post-test (n2=63). Most participants were between the ages of 41-50 years in both the pre-test (n1=40, 31%) and the post-test (n2=63, 33%). The majority of the participants worked more than 15 years as professional nurses (n1=47, 37%) in the pre-test findings and 11-15 years (n2=19, 30%) in the post-test findings. All the participants were trained in general nursing science in the pre-test (n1=128, 100%), as well as post-test (n2=63, 100%) and the majority of participants were trained in community nursing science in the pre-test (n1=106, 83%) and post-test (n2=58, 92%). However, the majority of participants have indicated to receive no informal intimate partner violence training in the pre-test test (n1=11, 9%) versus the informal training that most participants received in the post-test (n2=50, 79%). Tests scores for knowledge ranged between 29% (n1=37) and 29% (n1=120) in the pre-test and 27% (n2=17) and 94% (n2=59) in the post-test. Test scores for practice ranged between 7% (n1=8) and 46% (n1=59) in the pre-test and 10% (n2=6) and 65% (n2=41) in the post-test. The mean score for knowledge in the pre-test (n1=8,17) and the post-test (n2=8,83) were better than the practice scores in both the pre-test (n1=2,44) and the post-test (n2=2,68). The 20-minutes’ educational session made a slight difference regarding the knowledge as statistically a significant difference was found regarding knowledge between the pre-test and post-test (d.f.=,204; Cohens’d 0.29). However, there is still a gap in knowledge among professional nurses regarding IPV in the Nelson Mandela Bay Health District. Further recommendations for practice, research and education were given.
- Full Text:
- Date Issued: 2018
- Authors: Felix, Rehanna Theresa
- Date: 2018
- Subjects: Marital violence -- South Africa -- Psychological aspects , Nursing -- Social aspects Primary health care -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MCur
- Identifier: http://hdl.handle.net/10948/29816 , vital:30781
- Description: Intimate partner violence is a global concern that affects most countries. In South Africa women are mostly affected by intimate partner violence, which often results in physical injuries or mental disorders. The World Health Organization compiled guidelines to combat the problem of intimate partner violence against women with emphasis on screening for intimate partner violence. However, a screening tool to identify intimate partner violence in female patients was absent in the Nelson Mandela Bay Health District, resulting in cases of intimate partner violence being unidentified. The aim of the research study is to determine the knowledge and practices of professional nurses regarding intimate partner violence and to introduce the use of an intimate partner violence screening tool in primary healthcare facilities to assist professional nurses to identify intimate partner violence. The objectives were firstly, to determine the current knowledge and practices utilized by professional nurses to identify intimate partner violence; secondly, to introduce an intimate partner violence screening tool to identify intimate partner violence; thirdly, to evaluate the knowledge of the professional nurses obtained in the educational session and practices post-implementation of the intimate partner violence screening tool. The researcher conducted a small intervention research study using quantitative, quasi-experimental one group pre-and post-test design. An all-inclusive sample consisting of professional nurses working in primary healthcare facilities in Nelson Mandela Bay Health District was included in the study. The research study was conducted in three phases: Phase one, the pre-test determined the current knowledge and practices of the professional nurses to identify intimate partner violence. Phase two, entailed educational sessions to educate professional nurses that participated in Phase one regarding intimate partner violence and the intimate partner violence screening tool. Phase three, focused on the post-test to evaluate the knowledge of the professional nurses obtained in the educational session and practices post-implementation of the intimate partner violence screening tool. The data was analysed through descriptive and inferential statistics with assistance of the statistician using frequency distribution, central tendency, Chi-square, t-test, Cohens’d and Cramér’s V and Cronbach’s alpha. The researchers ensured reliability and validity throughout the study. The researcher applied the ethical principles of beneficence, non-maleficence iii and autonomy, as well as privacy and confidentiality. Ethical permission was obtained for the study. The response in the pre-test was (n1=128) and the post-test (n2=63). Most participants were between the ages of 41-50 years in both the pre-test (n1=40, 31%) and the post-test (n2=63, 33%). The majority of the participants worked more than 15 years as professional nurses (n1=47, 37%) in the pre-test findings and 11-15 years (n2=19, 30%) in the post-test findings. All the participants were trained in general nursing science in the pre-test (n1=128, 100%), as well as post-test (n2=63, 100%) and the majority of participants were trained in community nursing science in the pre-test (n1=106, 83%) and post-test (n2=58, 92%). However, the majority of participants have indicated to receive no informal intimate partner violence training in the pre-test test (n1=11, 9%) versus the informal training that most participants received in the post-test (n2=50, 79%). Tests scores for knowledge ranged between 29% (n1=37) and 29% (n1=120) in the pre-test and 27% (n2=17) and 94% (n2=59) in the post-test. Test scores for practice ranged between 7% (n1=8) and 46% (n1=59) in the pre-test and 10% (n2=6) and 65% (n2=41) in the post-test. The mean score for knowledge in the pre-test (n1=8,17) and the post-test (n2=8,83) were better than the practice scores in both the pre-test (n1=2,44) and the post-test (n2=2,68). The 20-minutes’ educational session made a slight difference regarding the knowledge as statistically a significant difference was found regarding knowledge between the pre-test and post-test (d.f.=,204; Cohens’d 0.29). However, there is still a gap in knowledge among professional nurses regarding IPV in the Nelson Mandela Bay Health District. Further recommendations for practice, research and education were given.
- Full Text:
- Date Issued: 2018
Knowledge and responsibility regarding teaching teenage girl’s reproductive healthcare: views of Eastern Cape Province teachers
- Authors: Hendricks, Thenjiwe
- Date: 2018
- Subjects: Reproductive health -- South Africa -- Eastern Cape , Teenage pregnancy -- South Africa -- Port Elizabeth High school teachers -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MCur
- Identifier: http://hdl.handle.net/10948/30094 , vital:30824
- Description: Teachers play a vital role in educating and teaching of children at school. One of the areas for teaching responsibility is development of the individual learner to be a competent community member, either as a professional or as an otherwise- skilled person. It is therefore for that reason that the South African government introduced into the curriculum at school level a subject that is directed at life orientation to empower learners. Teachers bear the responsibility of teaching this subject. One of the topics in the Life Orientation subject is Reproductive Healthcare, which is supposed to empower the learners especially with matters of sex and sexuality, diseases included. Despite the teaching of this topic in schools, the rate of teenage pregnancies and occurrence of sexually -transmitted infections remains on the increase in the country. Although much literature exists about teenage pregnancy in the country, there is theoretical paucity on how teachers view their responsibility to teach reproductive healthcare. The questions that prompted and have been raised in this study have been: How much do school teachers know about reproductive healthcare? How do school teachers view the responsibility given to them of teaching the reproductive healthcare? What kind of assistance is needed by the school teachers with regard to their responsibility towards teaching the reproductive healthcare topic? The objectives developed for the study were to: explore the knowledge of school teachers with regard to reproductive healthcare; explore and describe the views of school teachers with regard to their responsibility to teach the reproductive healthcare topic to teenage girls ;and develop guidelines for schools to assist school teachers with regard to their responsibility to teach the reproductive healthcare topic to teenage girls. Following the granting of the necessary university permission and approval from other relevant authorities, the study was conducted between May and June 2017, using a qualitative explorative, descriptive and contextual design. The research population and sample was school teachers specifically those who were working at public high schools, in the Sarah Baartman and Makana districts and Nelson Mandela Bay Municipal area. Furthermore, the teachers were from schools that were using the Life Orientation curriculum. Sampling was done purposively. Data was collected by means of focus-group semi-structured interviews that were captured by means of a digital voice-recorder and an interview schedule consisting of four questions. The number of participants ranged from four to five per focus group. Field notes were also written down to record non-verbal cues and events observed from the participants during the interview sessions. Altogether twenty teachers participated in this study. From the twenty participants six teachers were part of the pilot study and an additional fourteen were the ultimate sample size. From the fourteen participants four focus -group interviews were conducted. The collected data was analysed following the spiral data -analysis method as indicated by de Vos. As findings, three themes emerged and were the following. Teachers view their responsibility to teach reproductive healthcare to teenage girls as burdensome. Teachers identified the changes to the curriculum as a concern that was affecting their teaching of the topic. Furthermore, the teachers related positively to the need for teaching of the reproductive healthcare topic at schools. Based on these findings and the guidance of the model adopted for the study, that of the Health Promotion Model, three main guidelines were developed. These guidelines will teachers on how best to meet their responsibility of teaching the reproductive topic to teenage girls. The guidelines were to: create a set of core values that form the basis of responsibility to teach reproductive healthcare to teenage girls; devise a plan that will assist participants to deal with the immediate burden of teaching reproductive healthcare but also for future purposes and create an atmosphere that would increase the positivity of teachers towards teaching reproductive healthcare to teenage girls. Furthermore, recommendations for clinical nursing practice, nursing education and nursing research were developed. Trustworthiness of the study was maintained by using the criteria of credibility, transferability, dependability and confirmability. Ethical principles adopted in this study were those of beneficence, maleficence, justice and respect while applying the strategies of permission, informed consent, privacy, confidentiality and anonymity.
- Full Text:
- Date Issued: 2018
- Authors: Hendricks, Thenjiwe
- Date: 2018
- Subjects: Reproductive health -- South Africa -- Eastern Cape , Teenage pregnancy -- South Africa -- Port Elizabeth High school teachers -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MCur
- Identifier: http://hdl.handle.net/10948/30094 , vital:30824
- Description: Teachers play a vital role in educating and teaching of children at school. One of the areas for teaching responsibility is development of the individual learner to be a competent community member, either as a professional or as an otherwise- skilled person. It is therefore for that reason that the South African government introduced into the curriculum at school level a subject that is directed at life orientation to empower learners. Teachers bear the responsibility of teaching this subject. One of the topics in the Life Orientation subject is Reproductive Healthcare, which is supposed to empower the learners especially with matters of sex and sexuality, diseases included. Despite the teaching of this topic in schools, the rate of teenage pregnancies and occurrence of sexually -transmitted infections remains on the increase in the country. Although much literature exists about teenage pregnancy in the country, there is theoretical paucity on how teachers view their responsibility to teach reproductive healthcare. The questions that prompted and have been raised in this study have been: How much do school teachers know about reproductive healthcare? How do school teachers view the responsibility given to them of teaching the reproductive healthcare? What kind of assistance is needed by the school teachers with regard to their responsibility towards teaching the reproductive healthcare topic? The objectives developed for the study were to: explore the knowledge of school teachers with regard to reproductive healthcare; explore and describe the views of school teachers with regard to their responsibility to teach the reproductive healthcare topic to teenage girls ;and develop guidelines for schools to assist school teachers with regard to their responsibility to teach the reproductive healthcare topic to teenage girls. Following the granting of the necessary university permission and approval from other relevant authorities, the study was conducted between May and June 2017, using a qualitative explorative, descriptive and contextual design. The research population and sample was school teachers specifically those who were working at public high schools, in the Sarah Baartman and Makana districts and Nelson Mandela Bay Municipal area. Furthermore, the teachers were from schools that were using the Life Orientation curriculum. Sampling was done purposively. Data was collected by means of focus-group semi-structured interviews that were captured by means of a digital voice-recorder and an interview schedule consisting of four questions. The number of participants ranged from four to five per focus group. Field notes were also written down to record non-verbal cues and events observed from the participants during the interview sessions. Altogether twenty teachers participated in this study. From the twenty participants six teachers were part of the pilot study and an additional fourteen were the ultimate sample size. From the fourteen participants four focus -group interviews were conducted. The collected data was analysed following the spiral data -analysis method as indicated by de Vos. As findings, three themes emerged and were the following. Teachers view their responsibility to teach reproductive healthcare to teenage girls as burdensome. Teachers identified the changes to the curriculum as a concern that was affecting their teaching of the topic. Furthermore, the teachers related positively to the need for teaching of the reproductive healthcare topic at schools. Based on these findings and the guidance of the model adopted for the study, that of the Health Promotion Model, three main guidelines were developed. These guidelines will teachers on how best to meet their responsibility of teaching the reproductive topic to teenage girls. The guidelines were to: create a set of core values that form the basis of responsibility to teach reproductive healthcare to teenage girls; devise a plan that will assist participants to deal with the immediate burden of teaching reproductive healthcare but also for future purposes and create an atmosphere that would increase the positivity of teachers towards teaching reproductive healthcare to teenage girls. Furthermore, recommendations for clinical nursing practice, nursing education and nursing research were developed. Trustworthiness of the study was maintained by using the criteria of credibility, transferability, dependability and confirmability. Ethical principles adopted in this study were those of beneficence, maleficence, justice and respect while applying the strategies of permission, informed consent, privacy, confidentiality and anonymity.
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- Date Issued: 2018