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Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain

- Mossie, Godwin Mxolisi Kevin

  • Authors: Mossie, Godwin Mxolisi Kevin
  • Date: 1980
  • Subjects: Bacteroides , Anaerobic bacteria , Trypsin , Dinitrophenol , Proteins -- Synthesis
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4124 , http://hdl.handle.net/10962/d1013543
  • Description: Bacteroides fragilis strain Bf-1 produces an extracellular bacteriocin at the beginning of the stationary growth phase. Production is not inducible by either ultraviolet light or mitomycin C. The low molecular weight bacteriocin (MW estimates of 13 500 and 18 800 obtained from Sephadex G-100 chromatography and SDS-PAGE electrophoresis respecively) is stable between pH 7 - 9 and is inactivated on incubation with trypsin and pronase. An unusual feature of the Bf-1 bacteriocin is its apparent biphasic temperature stability: while the majority of the activity (97%) is destroyed by heating at 60ºC (t [subscript] 1/2 = 2.5 min at 60ºC), a small proportion (3%) is stable even after autoclaving at 121ºC for 15 min. The killing of sensitive cells occurs in 2 stages and the killing action is reversed by incubation with trypsin. The transition from stage I to stage II is dependent on the temperature of incubation and the growth state of sensitive cells. 2,4-Dinitrophenol prevents this transition. The Bf-1 bacteriocin has an unusual mode of action. It specifically inhibits RNA synthesis whilst having no effect on protein or DNA synthesis. No effect on intracellular ATP levels were observed. The heat-stable (3%) fraction had a similar biochemical effect. In vitro studies involving RNA polymerase indicated that the bacteriocin and the antibiotic rifampicin have similar effects on RNA synthesis. The bacteriocinogenic strain (Bf-1) is insensitive to its own bacteriocin both in vivo and in vitro, although this immunity is overcome in vitro by the addition of higher concentrations of the Bf-1 bacteriocin. The bacteriocinogenic strain (Bf-1) harbors a cryptic plasmid (or plasmids) which on a neutral sucrose gradient, sediments faster than the Col E1 marker plasmid DNA. Attempts to cure this strain of its bacteriocinogenic phenotype were unsuccessful.
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  • Date Issued: 1980

Christian joy

- Williams, Denis Ivor

  • Authors: Williams, Denis Ivor
  • Date: 1980
  • Subjects: Joy -- Religious aspects -- Christianity Christian life
  • Language: English
  • Type: Thesis , Doctoral , PhD
  • Identifier: vital:1212 , http://hdl.handle.net/10962/d1001541
  • Description: Christian joy is identified by linguistic and symbolic, experiential and psychological studies, and by a study of its opposite, sorrow. The final and most comprehensive approach is Biblical and theological. Here, through Judaism and Christianity, the genesis and fulfilment of Christian joy is examined, in life and the gifts of God, in hope, and in union with God. It is defined as "a gift of God's Holy Spirit as man becomes one with Christ in love." Five hypotheses are evaluated and confirmed: - 1. God is perfect joy, 2. God is the source and end of all Christian joy, 3. Jesus Christ is both the most joyful and the most sorrowful of men, 4. The Christian participates in the joy and sorrow of Jesus Christ, 5. Christian joy is eschatological in nature. The need is stressed for a fuller understanding and expression of Christian joy, which is seen as the complement of Christian love, and as a distinguishing characteristic of Christians, because of its primary intentional and ultimate satisfactory nature
  • Full Text:
  • Date Issued: 1980

Composer not specified

- Siswati church music workshop participants, Composer not specifed, Dargie, Dave

Consecration Acclamation

- Siswati church music workshop participants, Dlamini, Josephina, Dargie, Dave

Crado

- Music workshop participants, Group composition, Dargie, Dave

Credo

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Credo

- Children of the Nkosiyethu School, Sithole, Clement, OSB, Dargie, Dave

Credo

- Lumko church choir for Silver Jubilee of father Oswald and father Fritz, Composer not specified, Dargie, Dave

Credo

- Church choir, Composer not specified, Dargie, Dave

Credo

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Credo from the Missa Zimbabwe

- Louis Trichardt church music workshop participants, Composer not specified, Dargie, Dave

Creed: Missa Zimbabwe

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Creed: Missa Zimbabwe

- Lumko Church Congregation, Composer not specified, Dargie, Dave

Dead Yellowwood tree

- Skead, C J (Cuthbert John)

Decorated shield

- Performer not specified, Composer not specified, Dargie, Dave

Development of a high pressure liquid chromatographic method for the simultaneous analysis of sulphamethoxazole and trimethoprim and its application to biological fluids and dissolution rate studies on solid oral dosage forms

- Gochin, Rosa

  • Authors: Gochin, Rosa
  • Date: 1980
  • Subjects: High performance liquid chromatography , Body fluids -- Analysis , Drugs -- Dosage forms
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3735 , http://hdl.handle.net/10962/d1001524
  • Description: Co-trimoxazole, a combination of a 5-to-l ratio of Sulphamethoxazole (SMZ) and Trimethoprim (TMP) , is a highly effective, broad-spectrum antibacterial agent. Since its introduction in 1968, it has been extensively used in infections of the respiratory and urinary tracts. Co-trimoxazole was developed by the systematic investigation of a series of compounds whose mechanism of action was already known. As early as 1950 synergy between sulphonamides and 2,4-diaminopyrimidines was reported. This was to be expected as both groups of drugs exert their antibacterial activity by interfering with the same biochemical pathway in bacteria. TMP was chosen from among many 2,4-diaminopyrimidines tested because of its good antibacterial activity and low toxicity. SMZ was chosen from the sulphonamides available for combination with TMP because of similarity of their biological half-lives. The widespread use of the combination coupled with the fact that monitoring of the levels of all drugs in the body is becoming increasingly important has stimulated research into rapid and efficient methods for the analysis of TMP and SMZ in biological fluids. Another consequence of the immense popularity of the combination is the appearance on the market of several generic preparations of Co-trimoxazole. It is now generally recognized that drug products from different manufacturers which are chemically equivalent may not be therapeutically equivalent. This is due to the fact that the absorption rate and/or bioavailability (extent of absorption) of a poorly soluble drug may be markedly affected by its release rate from the product and by its subsequent dissolution rate in gastrointestinal fluids. Hence bioequivalence of these various products should be established
  • Full Text:
  • Date Issued: 1980

Directory of welfare, religious and service organisations, social and sporting clubs and other societies and councils in Grahamstown

- Henderson, Thelma M

Dumusani hosi

- Church choir, Kubayi. Daniel, Dargie, Dave

Ebelel engekho kwaphezolo

- Choir Group, Composer Not Specified, Dargie, Dave

Ebelel' engekho phezolo

- Performer not specified, Composer not specified, Dargie, Dave

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