Enhanced visualisation techniques to support access to personal information across multiple devices
- Authors: Beets, Simone Yvonne
- Date: 2014
- Subjects: Information visualisation , Database management , Web services , Personal information management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10500 , http://hdl.handle.net/10948/d1021136
- Description: The increasing number of devices owned by a single user makes it increasingly difficult to access, organise and visualise personal information (PI), i.e. documents and media, across these devices. The primary method that is currently used to organise and visualise PI is the hierarchical folder structure, which is a familiar and widely used means to manage PI. However, this hierarchy does not effectively support personal information management (PIM) across multiple devices. Current solutions, such as the Personal Information Dashboard and Stuff I’ve Seen, do not support PIM across multiple devices. Alternative PIM tools, such as Dropbox and TeamViewer, attempt to provide a means of accessing PI across multiple devices, but these solutions also suffer from several limitations. The aim of this research was to investigate to what extent enhanced information visualisation (IV) techniques could be used to support accessing PI across multiple devices. An interview study was conducted to identify how PI is currently managed across multiple devices. This interview study further motivated the need for a tool to support visualising PI across multiple devices and identified requirements for such an IV tool. Several suitable IV techniques were selected and enhanced to support PIM across multiple devices. These techniques comprised an Overview using a nested circles layout, a Tag Cloud and a Partition Layout, which used a novel set-based technique. A prototype, called MyPSI, was designed and implemented incorporating these enhanced IV techniques. The requirements and design of the MyPSI prototype were validated using a conceptual walkthrough. The design of the MyPSI prototype was initially implemented for a desktop or laptop device with mouse-based interaction. A sample personal space of information (PSI) was used to evaluate the prototype in a controlled user study. The user study was used to identify any usability problems with the MyPSI prototype. The results were highly positive and the participants agreed that such a tool could be useful in future. No major problems were identified with the prototype. The MyPSI prototype was then implemented on a mobile device, specifically an Android tablet device, using a similar design, but supporting touch-based interaction. Users were allowed to upload their own PSI using Dropbox, which was visualised by the MyPSI prototype. A field study was conducted following the Multi-dimensional In-depth Long-term Case Studies approach specifically designed for IV evaluation. The field study was conducted over a two-week period, evaluating both the desktop and mobile versions of the MyPSI prototype. Both versions received positive results, but the desktop version was slightly preferred over the mobile version, mainly due to familiarity and problems experienced with the mobile implementation. Design recommendations were derived to inform future designs of IV tools to support accessing PI across multiple devices. This research has shown that IV techniques can be enhanced to effectively support accessing PI across multiple devices. Future work will involve customising the MyPSI prototype for mobile phones and supporting additional platforms.
- Full Text:
- Date Issued: 2014
- Authors: Beets, Simone Yvonne
- Date: 2014
- Subjects: Information visualisation , Database management , Web services , Personal information management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10500 , http://hdl.handle.net/10948/d1021136
- Description: The increasing number of devices owned by a single user makes it increasingly difficult to access, organise and visualise personal information (PI), i.e. documents and media, across these devices. The primary method that is currently used to organise and visualise PI is the hierarchical folder structure, which is a familiar and widely used means to manage PI. However, this hierarchy does not effectively support personal information management (PIM) across multiple devices. Current solutions, such as the Personal Information Dashboard and Stuff I’ve Seen, do not support PIM across multiple devices. Alternative PIM tools, such as Dropbox and TeamViewer, attempt to provide a means of accessing PI across multiple devices, but these solutions also suffer from several limitations. The aim of this research was to investigate to what extent enhanced information visualisation (IV) techniques could be used to support accessing PI across multiple devices. An interview study was conducted to identify how PI is currently managed across multiple devices. This interview study further motivated the need for a tool to support visualising PI across multiple devices and identified requirements for such an IV tool. Several suitable IV techniques were selected and enhanced to support PIM across multiple devices. These techniques comprised an Overview using a nested circles layout, a Tag Cloud and a Partition Layout, which used a novel set-based technique. A prototype, called MyPSI, was designed and implemented incorporating these enhanced IV techniques. The requirements and design of the MyPSI prototype were validated using a conceptual walkthrough. The design of the MyPSI prototype was initially implemented for a desktop or laptop device with mouse-based interaction. A sample personal space of information (PSI) was used to evaluate the prototype in a controlled user study. The user study was used to identify any usability problems with the MyPSI prototype. The results were highly positive and the participants agreed that such a tool could be useful in future. No major problems were identified with the prototype. The MyPSI prototype was then implemented on a mobile device, specifically an Android tablet device, using a similar design, but supporting touch-based interaction. Users were allowed to upload their own PSI using Dropbox, which was visualised by the MyPSI prototype. A field study was conducted following the Multi-dimensional In-depth Long-term Case Studies approach specifically designed for IV evaluation. The field study was conducted over a two-week period, evaluating both the desktop and mobile versions of the MyPSI prototype. Both versions received positive results, but the desktop version was slightly preferred over the mobile version, mainly due to familiarity and problems experienced with the mobile implementation. Design recommendations were derived to inform future designs of IV tools to support accessing PI across multiple devices. This research has shown that IV techniques can be enhanced to effectively support accessing PI across multiple devices. Future work will involve customising the MyPSI prototype for mobile phones and supporting additional platforms.
- Full Text:
- Date Issued: 2014
An investigation into XSets of primitive behaviours for emergent behaviour in stigmergic and message passing antlike agents
- Authors: Chibaya, Colin
- Date: 2014
- Subjects: Ants -- Behavior -- Computer programs , Insects -- Behavior -- Computer programs , Ant communities -- Behavior , Insect societies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4698 , http://hdl.handle.net/10962/d1012965
- Description: Ants are fascinating creatures - not so much because they are intelligent on their own, but because as a group they display compelling emergent behaviour (the extent to which one observes features in a swarm which cannot be traced back to the actions of swarm members). What does each swarm member do which allows deliberate engineering of emergent behaviour? We investigate the development of a language for programming swarms of ant agents towards desired emergent behaviour. Five aspects of stigmergic (pheromone sensitive computational devices in which a non-symbolic form of communication that is indirectly mediated via the environment arises) and message passing ant agents (computational devices which rely on implicit communication spaces in which direction vectors are shared one-on-one) are studied. First, we investigate the primitive behaviours which characterize ant agents' discrete actions at individual levels. Ten such primitive behaviours are identified as candidate building blocks of the ant agent language sought. We then study mechanisms in which primitive behaviours are put together into XSets (collection of primitive behaviours, parameter values, and meta information which spells out how and when primitive behaviours are used). Various permutations of XSets are possible which define the search space for best performer XSets for particular tasks. Genetic programming principles are proposed as a search strategy for best performer XSets that would allow particular emergent behaviour to occur. XSets in the search space are evolved over various genetic generations and tested for abilities to allow path finding (as proof of concept). XSets are ranked according to the indices of merit (fitness measures which indicate how well XSets allow particular emergent behaviour to occur) they achieve. Best performer XSets for the path finding task are identifed and reported. We validate the results yield when best performer XSets are used with regard to normality, correlation, similarities in variation, and similarities between mean performances over time. Commonly, the simulation results yield pass most statistical tests. The last aspect we study is the application of best performer XSets to different problem tasks. Five experiments are administered in this regard. The first experiment assesses XSets' abilities to allow multiple targets location (ant agents' abilities to locate continuous regions of targets), and found out that best performer XSets are problem independent. However both categories of XSets are sensitive to changes in agent density. We test the influences of individual primitive behaviours and the effects of the sequences of primitive behaviours to the indices of merit of XSets and found out that most primitive behaviours are indispensable, especially when specific sequences are prescribed. The effects of pheromone dissipation to the indices of merit of stigmergic XSets are also scrutinized. Precisely, dissipation is not causal. Rather, it enhances convergence. Overall, this work successfully identify the discrete primitive behaviours of stigmergic and message passing ant-like devices. It successfully put these primitive behaviours together into XSets which characterize a language for programming ant-like devices towards desired emergent behaviour. This XSets approach is a new ant language representation with which a wider domain of emergent tasks can be resolved.
- Full Text:
- Date Issued: 2014
- Authors: Chibaya, Colin
- Date: 2014
- Subjects: Ants -- Behavior -- Computer programs , Insects -- Behavior -- Computer programs , Ant communities -- Behavior , Insect societies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4698 , http://hdl.handle.net/10962/d1012965
- Description: Ants are fascinating creatures - not so much because they are intelligent on their own, but because as a group they display compelling emergent behaviour (the extent to which one observes features in a swarm which cannot be traced back to the actions of swarm members). What does each swarm member do which allows deliberate engineering of emergent behaviour? We investigate the development of a language for programming swarms of ant agents towards desired emergent behaviour. Five aspects of stigmergic (pheromone sensitive computational devices in which a non-symbolic form of communication that is indirectly mediated via the environment arises) and message passing ant agents (computational devices which rely on implicit communication spaces in which direction vectors are shared one-on-one) are studied. First, we investigate the primitive behaviours which characterize ant agents' discrete actions at individual levels. Ten such primitive behaviours are identified as candidate building blocks of the ant agent language sought. We then study mechanisms in which primitive behaviours are put together into XSets (collection of primitive behaviours, parameter values, and meta information which spells out how and when primitive behaviours are used). Various permutations of XSets are possible which define the search space for best performer XSets for particular tasks. Genetic programming principles are proposed as a search strategy for best performer XSets that would allow particular emergent behaviour to occur. XSets in the search space are evolved over various genetic generations and tested for abilities to allow path finding (as proof of concept). XSets are ranked according to the indices of merit (fitness measures which indicate how well XSets allow particular emergent behaviour to occur) they achieve. Best performer XSets for the path finding task are identifed and reported. We validate the results yield when best performer XSets are used with regard to normality, correlation, similarities in variation, and similarities between mean performances over time. Commonly, the simulation results yield pass most statistical tests. The last aspect we study is the application of best performer XSets to different problem tasks. Five experiments are administered in this regard. The first experiment assesses XSets' abilities to allow multiple targets location (ant agents' abilities to locate continuous regions of targets), and found out that best performer XSets are problem independent. However both categories of XSets are sensitive to changes in agent density. We test the influences of individual primitive behaviours and the effects of the sequences of primitive behaviours to the indices of merit of XSets and found out that most primitive behaviours are indispensable, especially when specific sequences are prescribed. The effects of pheromone dissipation to the indices of merit of stigmergic XSets are also scrutinized. Precisely, dissipation is not causal. Rather, it enhances convergence. Overall, this work successfully identify the discrete primitive behaviours of stigmergic and message passing ant-like devices. It successfully put these primitive behaviours together into XSets which characterize a language for programming ant-like devices towards desired emergent behaviour. This XSets approach is a new ant language representation with which a wider domain of emergent tasks can be resolved.
- Full Text:
- Date Issued: 2014
A sociological study of employees' perceptions of electronic surveillance in public FET institutions in the Eastern Cape Province, South Africa
- Authors: Jonas, Randolph Patrick
- Date: 2014
- Subjects: Electronic monitoring in the workplace -- South Africa -- Eastern Cape , Privacy, Right of -- South Africa -- Eastern Cape , Personnel management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:16154 , http://hdl.handle.net/10948/d1020623
- Description: The perceptions of employees regarding electronic surveillance in the workplace were studied using a mixed method approach. The change that permeates society and organisations have not escaped public FET institutions in South Africa. These changes such as computerisation of work and the employment of electronic surveillance, have implications for management, control, privacy, productivity and trust in the workplace. Yet, public FET institutions are not performing to the expected standards. Surveillance of work and employees at these institutions are interventions of management to ensure organisational goals are achieved. Despite the prevalence of electronic surveillance in the workplace, employee perceptions and their lived experiences thereof are seldom reported. Published research barely focuses on employees’ perceptions of electronic surveillance in the workplace, and instead offers a perfunctory look at the perceptual dimensions. Employees’ perceptions reflects their lived experiences in the workplace and yield a foundational understanding of workplace dynamics and organisational behaviour. The perceptions of employees regarding electronic surveillance were viewed through the lens of quantitative and qualitative analysis to give statistical and thick descriptions respectively to augment better understanding. The study therefore provides a descriptive account of employees’ perceptions of electronic surveillance and its effects. Specifically the study examined employee perceptions of electronic surveillance as a control measure of management, as an intrusion of employee privacy and impacting on the trust relationship and productivity. An extensive review of the literature provided the basis for the research questions and eight hypotheses were proposed. A survey, by means of an electronic questionnaire, was conducted to test the hypotheses using a random sample of employees at three public FET institutions in the Eastern Cape Province, South Africa. The results are presented followed by a discussion, conclusions and limitations. Suggestions and recommendations for future research are also indicated. The central thesis of the study is presented, namely that electronic surveillance is experienced as a measure of management to control workers and that privacy as well trust, as an element of the employment relationship, are influenced by it. Further findings indicated that there are no statistically significant differences in the responses of the three job categories namely lecturers, managers and administration and support staff. Interviews were also conducted and the data from the interviews reported the depth and breadth of the perceptions of a small sample of employees who related their experiences and situations in terms of the key themes of electronic surveillance as control and an intrusion of their right to privacy, trust and productivity. The findings indicate that acceptance of electronic surveillance is contingent upon contextual factors and the meaning people assign to their situations. Privacy concerns were limited to personal information, fairness and dignity. The link between electronic surveillance and control and discipline is reported in the responses. The findings indicate that perceptions of electronic surveillance as managerial control is related to the lived experiences of employees. Employees voiced positive perceptions of electronic surveillance in cases where management clearly communicates the purpose to the employees. The study of the subjective experiences of employees in workplaces under electronic surveillance thus helped to illuminate the intricacies of employment relationships in the changing world of work. The study findings widen the knowledge base on organisational behaviour and is essential for human resources management and organisational development interventions. Human rights and ethics are part of the sense making process when employees construct social reality. Management and control is redefined in various terms and has implications for change management strategies and organisational development practises in globalising and network society. The study raises the critical question whether electronic surveillance as an adjunct for bureaucratic control is still relevant in a changing world of work where good faith, trust and confidence are still highly valued. The insights into the role of trust in the employment relationship is important for managing public institutions in complex settings. Management must be aware of the differences in the lived experiences of employees when dealing with workplace issues.
- Full Text:
- Date Issued: 2014
- Authors: Jonas, Randolph Patrick
- Date: 2014
- Subjects: Electronic monitoring in the workplace -- South Africa -- Eastern Cape , Privacy, Right of -- South Africa -- Eastern Cape , Personnel management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:16154 , http://hdl.handle.net/10948/d1020623
- Description: The perceptions of employees regarding electronic surveillance in the workplace were studied using a mixed method approach. The change that permeates society and organisations have not escaped public FET institutions in South Africa. These changes such as computerisation of work and the employment of electronic surveillance, have implications for management, control, privacy, productivity and trust in the workplace. Yet, public FET institutions are not performing to the expected standards. Surveillance of work and employees at these institutions are interventions of management to ensure organisational goals are achieved. Despite the prevalence of electronic surveillance in the workplace, employee perceptions and their lived experiences thereof are seldom reported. Published research barely focuses on employees’ perceptions of electronic surveillance in the workplace, and instead offers a perfunctory look at the perceptual dimensions. Employees’ perceptions reflects their lived experiences in the workplace and yield a foundational understanding of workplace dynamics and organisational behaviour. The perceptions of employees regarding electronic surveillance were viewed through the lens of quantitative and qualitative analysis to give statistical and thick descriptions respectively to augment better understanding. The study therefore provides a descriptive account of employees’ perceptions of electronic surveillance and its effects. Specifically the study examined employee perceptions of electronic surveillance as a control measure of management, as an intrusion of employee privacy and impacting on the trust relationship and productivity. An extensive review of the literature provided the basis for the research questions and eight hypotheses were proposed. A survey, by means of an electronic questionnaire, was conducted to test the hypotheses using a random sample of employees at three public FET institutions in the Eastern Cape Province, South Africa. The results are presented followed by a discussion, conclusions and limitations. Suggestions and recommendations for future research are also indicated. The central thesis of the study is presented, namely that electronic surveillance is experienced as a measure of management to control workers and that privacy as well trust, as an element of the employment relationship, are influenced by it. Further findings indicated that there are no statistically significant differences in the responses of the three job categories namely lecturers, managers and administration and support staff. Interviews were also conducted and the data from the interviews reported the depth and breadth of the perceptions of a small sample of employees who related their experiences and situations in terms of the key themes of electronic surveillance as control and an intrusion of their right to privacy, trust and productivity. The findings indicate that acceptance of electronic surveillance is contingent upon contextual factors and the meaning people assign to their situations. Privacy concerns were limited to personal information, fairness and dignity. The link between electronic surveillance and control and discipline is reported in the responses. The findings indicate that perceptions of electronic surveillance as managerial control is related to the lived experiences of employees. Employees voiced positive perceptions of electronic surveillance in cases where management clearly communicates the purpose to the employees. The study of the subjective experiences of employees in workplaces under electronic surveillance thus helped to illuminate the intricacies of employment relationships in the changing world of work. The study findings widen the knowledge base on organisational behaviour and is essential for human resources management and organisational development interventions. Human rights and ethics are part of the sense making process when employees construct social reality. Management and control is redefined in various terms and has implications for change management strategies and organisational development practises in globalising and network society. The study raises the critical question whether electronic surveillance as an adjunct for bureaucratic control is still relevant in a changing world of work where good faith, trust and confidence are still highly valued. The insights into the role of trust in the employment relationship is important for managing public institutions in complex settings. Management must be aware of the differences in the lived experiences of employees when dealing with workplace issues.
- Full Text:
- Date Issued: 2014
Coordinating legal aid services in civil matters for indigent people in eThekwini: a model for improved access to justice
- Authors: Holness, David Roy
- Date: 2014
- Subjects: Legal assistance to the poor -- South Africa Legal aid services -- South Africa Civil procedure -- South Africa
- Language: English
- Type: Thesis , Doctoral , LLD
- Identifier: http://hdl.handle.net/10948/10981 , vital:26864
- Description: The South African law and legal system can and should be a mechanism through which the lives of all resident there are enhanced through the safeguarding and advancement of the fundamental rights guaranteed in its Bill of Rights. This thesis focuses on ‘legal aid’ service delivery (broadly speaking) for the indigent in a particular locality by and through registered law clinics, other legal services providers and via other means in civil rather than criminal matters. In this regard there continue to be very substantial differences between the proper access to civil justice requirements of constitutional South Africa and the actual situation which has existed since the dawn of South Africa’s democratic era which continues unabated. Justice and equality are promised to all in South Africa, yet due to gaps in the ‘net’ of free legal services provided to the indigent, the ability to pay for legal services in civil cases often remains the deciding factor. This study examines the constitutional obligations which, it is argued, apply to the provision of free civil legal services to impoverished people in South Africa. This research considers the law as a vector for necessary positive transformation in the daily lives of those resident in South Africa, which is considered within the country’s woefully unequal socio-economic situation. It builds upon existing research and court authority which show the function of access to justice as an important promoter of the type of society envisaged by the South African Constitution - one where the enjoyment of justice and equality are within the reach of all. However, at present, if one can afford the expensive services of lawyers in civil matters, then access to justice is far more readily attainable. But the opposite is true where someone is denied meaningful access to justice through a lack of legal representation because they cannot afford prohibitively high lawyers’ costs (and disbursements) and no adequate alternatives are provided for by the state or through other means. In these circumstances a vulnerable, unrepresented litigant in a civil case faces a greatly increased likelihood of being denied proper access to a daunting and intricate legal system. There are two main reasons for concentrating on free legal services to the ‘needy’ in civil rather than criminal matters. In the first place, all available statistics show that a huge proportion of legal aid services in South Africa has been and continues to be dispensed in criminal rather than civil cases. Secondly, there has been minimal research or case authority in South Africa on legal aid and other free legal services for impoverished people in civil matters. This thesis examines the state of free civil legal service provision and the need for such assistance within the eThekwini Metropolitan Municipality, one of South Africa’s largest metropoles. This analysis includes an empirical study of the requests for free civil legal services in a particular year by qualifying potential clients in eThekwini and the degree to which free legal service providers are meeting or failing to meet those needs. The study considers the legal service provision in such matters by legal non-governmental organisations, state-supported legal service providers and the work of legal professionals in private practice acting pro bono. The thesis then proposes a model for eThekwini for coordinating (and concurrently improving) civil legal aid services, pro bono legal work and other forms of free legal assistance - like community service by senior law students and law graduates - in response to the particular needs and circumstances facing the indigent there. When referring to the concept of ‘legal aid services’, this research concentrates on legal advice, assistance and representation to indigent clients. However, the promotion of legal rights awareness to such clients is often necessary to open their eyes to the possibility of legal avenues, where appropriate, to improve their situations. Therefore this study also considers - albeit to a lesser degree - this more indirect form of legal assistance through the dissemination of legal knowledge in an accessible form to clients who would qualify for legal aid assistance. The work concludes by briefly postulating the likely appropriateness (and/or limitations) of the aforementioned ‘free civil legal service model for the indigent’ beyond eThekwini.
- Full Text:
- Date Issued: 2014
- Authors: Holness, David Roy
- Date: 2014
- Subjects: Legal assistance to the poor -- South Africa Legal aid services -- South Africa Civil procedure -- South Africa
- Language: English
- Type: Thesis , Doctoral , LLD
- Identifier: http://hdl.handle.net/10948/10981 , vital:26864
- Description: The South African law and legal system can and should be a mechanism through which the lives of all resident there are enhanced through the safeguarding and advancement of the fundamental rights guaranteed in its Bill of Rights. This thesis focuses on ‘legal aid’ service delivery (broadly speaking) for the indigent in a particular locality by and through registered law clinics, other legal services providers and via other means in civil rather than criminal matters. In this regard there continue to be very substantial differences between the proper access to civil justice requirements of constitutional South Africa and the actual situation which has existed since the dawn of South Africa’s democratic era which continues unabated. Justice and equality are promised to all in South Africa, yet due to gaps in the ‘net’ of free legal services provided to the indigent, the ability to pay for legal services in civil cases often remains the deciding factor. This study examines the constitutional obligations which, it is argued, apply to the provision of free civil legal services to impoverished people in South Africa. This research considers the law as a vector for necessary positive transformation in the daily lives of those resident in South Africa, which is considered within the country’s woefully unequal socio-economic situation. It builds upon existing research and court authority which show the function of access to justice as an important promoter of the type of society envisaged by the South African Constitution - one where the enjoyment of justice and equality are within the reach of all. However, at present, if one can afford the expensive services of lawyers in civil matters, then access to justice is far more readily attainable. But the opposite is true where someone is denied meaningful access to justice through a lack of legal representation because they cannot afford prohibitively high lawyers’ costs (and disbursements) and no adequate alternatives are provided for by the state or through other means. In these circumstances a vulnerable, unrepresented litigant in a civil case faces a greatly increased likelihood of being denied proper access to a daunting and intricate legal system. There are two main reasons for concentrating on free legal services to the ‘needy’ in civil rather than criminal matters. In the first place, all available statistics show that a huge proportion of legal aid services in South Africa has been and continues to be dispensed in criminal rather than civil cases. Secondly, there has been minimal research or case authority in South Africa on legal aid and other free legal services for impoverished people in civil matters. This thesis examines the state of free civil legal service provision and the need for such assistance within the eThekwini Metropolitan Municipality, one of South Africa’s largest metropoles. This analysis includes an empirical study of the requests for free civil legal services in a particular year by qualifying potential clients in eThekwini and the degree to which free legal service providers are meeting or failing to meet those needs. The study considers the legal service provision in such matters by legal non-governmental organisations, state-supported legal service providers and the work of legal professionals in private practice acting pro bono. The thesis then proposes a model for eThekwini for coordinating (and concurrently improving) civil legal aid services, pro bono legal work and other forms of free legal assistance - like community service by senior law students and law graduates - in response to the particular needs and circumstances facing the indigent there. When referring to the concept of ‘legal aid services’, this research concentrates on legal advice, assistance and representation to indigent clients. However, the promotion of legal rights awareness to such clients is often necessary to open their eyes to the possibility of legal avenues, where appropriate, to improve their situations. Therefore this study also considers - albeit to a lesser degree - this more indirect form of legal assistance through the dissemination of legal knowledge in an accessible form to clients who would qualify for legal aid assistance. The work concludes by briefly postulating the likely appropriateness (and/or limitations) of the aforementioned ‘free civil legal service model for the indigent’ beyond eThekwini.
- Full Text:
- Date Issued: 2014
A framework for business leadership in Africa
- Authors: Whitley, Elwyn
- Date: 2014
- Subjects: Leadership -- Africa Success in business , Management -- Africa Business Industrial management -- Africa
- Language: English
- Type: Thesis , Doctoral , DBA
- Identifier: http://hdl.handle.net/10948/45814 , vital:39219
- Description: In a fast moving, rapidly changing and highly competitive world the importance of strong leadership in both government and business cannot be over emphasised. The realisation that leadership is necessary for the organisation’s success and is key for the organisation’s survival (Alimo-Metcalfe and Alban- Metcalfe, 2008) is evident in the increasing focus on the concept over the last three to four decades. As the world becomes more of a ‘global village’ adapting to doing business in this new environment will require a leader who not only has the traditional skills set but also has the additional knowledge, skills and “mindset to navigate through the complexities brought on by moving beyond one's traditional borders” (Cohen, 2010. p. 3). This is of particular importance to Africa with the influx of foreign investors attracted by the growth opportunities that Africa offers, looking to expand their markets and in doing so imposing western norms and standards on local operations, in complex environments. Unfortunately Western leadership theories have not always been successful as Africans have found that in order to embrace Western ideals they need to relinquish some of their own beliefs. This highlights an opportunity to explore a possible hybrid leadership approach that harmonises the Western approach that is based on facts, logic and the nature of reality with the African humanistic orientation. The main aim of this study is to research the concept, principles, and characteristics of a small sample of business leaders in Africa in order to identify the factors that contribute to the leader’s success in a global business operating in Africa. By applying qualitative research methodology which includes an individual narrative written by each Managing Director, semistructured interviews and focus groups, a framework for business leadership in Africa was developed.
- Full Text:
- Date Issued: 2014
- Authors: Whitley, Elwyn
- Date: 2014
- Subjects: Leadership -- Africa Success in business , Management -- Africa Business Industrial management -- Africa
- Language: English
- Type: Thesis , Doctoral , DBA
- Identifier: http://hdl.handle.net/10948/45814 , vital:39219
- Description: In a fast moving, rapidly changing and highly competitive world the importance of strong leadership in both government and business cannot be over emphasised. The realisation that leadership is necessary for the organisation’s success and is key for the organisation’s survival (Alimo-Metcalfe and Alban- Metcalfe, 2008) is evident in the increasing focus on the concept over the last three to four decades. As the world becomes more of a ‘global village’ adapting to doing business in this new environment will require a leader who not only has the traditional skills set but also has the additional knowledge, skills and “mindset to navigate through the complexities brought on by moving beyond one's traditional borders” (Cohen, 2010. p. 3). This is of particular importance to Africa with the influx of foreign investors attracted by the growth opportunities that Africa offers, looking to expand their markets and in doing so imposing western norms and standards on local operations, in complex environments. Unfortunately Western leadership theories have not always been successful as Africans have found that in order to embrace Western ideals they need to relinquish some of their own beliefs. This highlights an opportunity to explore a possible hybrid leadership approach that harmonises the Western approach that is based on facts, logic and the nature of reality with the African humanistic orientation. The main aim of this study is to research the concept, principles, and characteristics of a small sample of business leaders in Africa in order to identify the factors that contribute to the leader’s success in a global business operating in Africa. By applying qualitative research methodology which includes an individual narrative written by each Managing Director, semistructured interviews and focus groups, a framework for business leadership in Africa was developed.
- Full Text:
- Date Issued: 2014
Tourism management in selected municipalities in the Eden District: a theoretical study
- Authors: Lamont, Andrew John
- Date: 2014
- Subjects: Sustainable tourism -- South Africa , Tourism -- South Africa -- Management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/8025 , vital:24460
- Description: For attainment sustainable tourism requires the growth of tourism‟s contribution to the economy and society as well as the sustainable use of resources and the environment. The achievement of both relies on dependable and sound understanding, appropriate development and management of the tourism demand. As demand patterns and economic structures change, no industry, and in particular no industry at the national or regional level, could or should assume that there is a constant or increasing flow of demand for its outputs and thus focus solely on resource issues (Butler, 1996). According to Elliot (1997:19) local government plays an important role in tourism, as it is only governments that have the authority to provide political stability, security and the legal and financial framework which tourism requires. Connell et al (2009) argue that one of the more important roles of local government is the development and implementation of community planning, legislation and policy. However, while tourism-related developments are usually integrated with economic development or official community plans, often there is no guiding policy framework or identified area of responsibility for tourism, which can be problematic for effective and sustainable tourism development. The effectiveness of local tourism governance in achieving the goals of sustainable tourism depends on the effectiveness of institutional structures and processes, and the rational resources and skill sets available (Healy, 2006). This study examines the arrangements of selected municipalities and the effectiveness of their local tourism governance and its relevance to sustainable tourism.
- Full Text:
- Date Issued: 2014
- Authors: Lamont, Andrew John
- Date: 2014
- Subjects: Sustainable tourism -- South Africa , Tourism -- South Africa -- Management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/8025 , vital:24460
- Description: For attainment sustainable tourism requires the growth of tourism‟s contribution to the economy and society as well as the sustainable use of resources and the environment. The achievement of both relies on dependable and sound understanding, appropriate development and management of the tourism demand. As demand patterns and economic structures change, no industry, and in particular no industry at the national or regional level, could or should assume that there is a constant or increasing flow of demand for its outputs and thus focus solely on resource issues (Butler, 1996). According to Elliot (1997:19) local government plays an important role in tourism, as it is only governments that have the authority to provide political stability, security and the legal and financial framework which tourism requires. Connell et al (2009) argue that one of the more important roles of local government is the development and implementation of community planning, legislation and policy. However, while tourism-related developments are usually integrated with economic development or official community plans, often there is no guiding policy framework or identified area of responsibility for tourism, which can be problematic for effective and sustainable tourism development. The effectiveness of local tourism governance in achieving the goals of sustainable tourism depends on the effectiveness of institutional structures and processes, and the rational resources and skill sets available (Healy, 2006). This study examines the arrangements of selected municipalities and the effectiveness of their local tourism governance and its relevance to sustainable tourism.
- Full Text:
- Date Issued: 2014
Adherence to antiretroviral therapy in children in Zimbabwe: a randomized control trial to validate a new self-reported adherence monitoring tool
- Authors: Mugore, Linnetie
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54734 , vital:26607
- Description: Background: Among children taking antiretroviral therapy (ART), self-reports have been widely reported to over-estimate adherence levels. Pill count adherence levels are often lower than self-reported levels, with unannounced home pill count adherence being lower than facility based pill count adherence. There is often poor agreement between pill count adherence levels and those measured using other objective adherence measuring methods such as Medication Event Monitoring Systems (MEMS®), which is widely viewed as the gold standard for adherence measurement. Objectives: The aim of this study was to design and evaluate a new self-reported paediatric adherence monitoring tool, assess the feasibility of using pill count methods in monitoring adherence and identify challenges to reporting adherence among children on ART in rural and urban Zimbabwe. Methods A dual centre, superiority, parallel design RCT was conducted to evaluate the newly-developed visually- and verbally-cued „past 10 days‟ tool for the assessment of adherence in children on ART at two sites in Zimbabwe; Harare Central Children‟s Hospital in an urban setting, and Murambinda Mission Hospital, a rural site. Child-caregiver pairs presenting to one of these facilities for the child‟s review of ART and refill of the medication were recruited, signed informed consent obtained, and were randomised for self-reported adherence monitoring into either the experimental group („new 10-day tool‟) or the control group („PACTG-style‟ self-report tool). Data (demographic, socioeconomic, and reported adherence) were collected in individual interviews with child-caregiver pairs. Additional adherence monitoring methods used for both groups included the Morisky-8-Item Medication Adherence Scale (MMAS-8) and a facility based pill count. FGDs were held with groups of caregivers and groups of children ≥13 years of age to understand reasons for non-adherence as well as issues around reporting non-adherence. Superiority testing was conducted by comparing adherent proportions and their confidence intervals (95% CI). Further concurrent validity test was done using the Mann-Whitney U test to evaluate the relationship between the new tool and the MMAS-8 scores. Agreement between the child and caregiver reports of adherence was used as a test of reliability of the new tool using the kappa statistic. Socio-demographic, clinical and care-related factors associated with adherence were identified using reported adherence in both child and caregiver groups in a logistic regression model. Two pill count methods were assessed for feasibility using the proportions of children with complete data for calculating adherence levels, and their CI and a comparison of the two methods, a routinely-used method and one that incorporated the reported residual quantity (RRQ) of medication at last refill. Results : Analysis included 245 child-caregiver pairs, 123 in the experimental group and 122 in the control group. The median age for children was 9 years. In the experimental group, adherence by caregiver and child reports ranged from 94.3% - 98.4% and 78.4% - 96.1%, and those in the control group ranged from 89.2% - 97.5% and 71.2% - 98.1%, respectively. There was no significant difference between adherence levels in the two groups. Adherence levels measured by both the experimental and control tools were found to be associated with MMAS-8 adherence levels (p <0.05). Agreement between child- and caregiver-reported adherence was moderate though significant (kappa; 0.407, p <0.05). Only about half of the children had adequate data to compute pill counts. Proportions adherent at 95% cut-off were 39% by the „routine pill count‟ and 58% by the „Pill count RRQ‟. Being an orphan was associated with child reported-adherence whereas use of non-human reminders, having a maternal relative as a primary caregiver and knowledge of dose frequency, were all associated with caregiver-reported adherence. Major causes of non-adherence mentioned during the FGDs included interference of medication administration times with scheduling of routine socio-economic activities and lack of support from some non-biological caregivers. Reporting of non-adherence appeared to be hampered by perceptions of negative reactions by healthcare workers to these reports and by caregivers being unaware that the child missed some doses. Conclusions: The „new 10-day tool‟ was not shown to be superior to the „PACTG-style tool‟ in detecting non-adherence, however this new tool was found to be a valid and reliable adherence monitoring tool that included a moderately long recall period of 10 days, can be applied without the need for the respondent to remember names of individual medicines in the
- Full Text:
- Date Issued: 2014
- Authors: Mugore, Linnetie
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54734 , vital:26607
- Description: Background: Among children taking antiretroviral therapy (ART), self-reports have been widely reported to over-estimate adherence levels. Pill count adherence levels are often lower than self-reported levels, with unannounced home pill count adherence being lower than facility based pill count adherence. There is often poor agreement between pill count adherence levels and those measured using other objective adherence measuring methods such as Medication Event Monitoring Systems (MEMS®), which is widely viewed as the gold standard for adherence measurement. Objectives: The aim of this study was to design and evaluate a new self-reported paediatric adherence monitoring tool, assess the feasibility of using pill count methods in monitoring adherence and identify challenges to reporting adherence among children on ART in rural and urban Zimbabwe. Methods A dual centre, superiority, parallel design RCT was conducted to evaluate the newly-developed visually- and verbally-cued „past 10 days‟ tool for the assessment of adherence in children on ART at two sites in Zimbabwe; Harare Central Children‟s Hospital in an urban setting, and Murambinda Mission Hospital, a rural site. Child-caregiver pairs presenting to one of these facilities for the child‟s review of ART and refill of the medication were recruited, signed informed consent obtained, and were randomised for self-reported adherence monitoring into either the experimental group („new 10-day tool‟) or the control group („PACTG-style‟ self-report tool). Data (demographic, socioeconomic, and reported adherence) were collected in individual interviews with child-caregiver pairs. Additional adherence monitoring methods used for both groups included the Morisky-8-Item Medication Adherence Scale (MMAS-8) and a facility based pill count. FGDs were held with groups of caregivers and groups of children ≥13 years of age to understand reasons for non-adherence as well as issues around reporting non-adherence. Superiority testing was conducted by comparing adherent proportions and their confidence intervals (95% CI). Further concurrent validity test was done using the Mann-Whitney U test to evaluate the relationship between the new tool and the MMAS-8 scores. Agreement between the child and caregiver reports of adherence was used as a test of reliability of the new tool using the kappa statistic. Socio-demographic, clinical and care-related factors associated with adherence were identified using reported adherence in both child and caregiver groups in a logistic regression model. Two pill count methods were assessed for feasibility using the proportions of children with complete data for calculating adherence levels, and their CI and a comparison of the two methods, a routinely-used method and one that incorporated the reported residual quantity (RRQ) of medication at last refill. Results : Analysis included 245 child-caregiver pairs, 123 in the experimental group and 122 in the control group. The median age for children was 9 years. In the experimental group, adherence by caregiver and child reports ranged from 94.3% - 98.4% and 78.4% - 96.1%, and those in the control group ranged from 89.2% - 97.5% and 71.2% - 98.1%, respectively. There was no significant difference between adherence levels in the two groups. Adherence levels measured by both the experimental and control tools were found to be associated with MMAS-8 adherence levels (p <0.05). Agreement between child- and caregiver-reported adherence was moderate though significant (kappa; 0.407, p <0.05). Only about half of the children had adequate data to compute pill counts. Proportions adherent at 95% cut-off were 39% by the „routine pill count‟ and 58% by the „Pill count RRQ‟. Being an orphan was associated with child reported-adherence whereas use of non-human reminders, having a maternal relative as a primary caregiver and knowledge of dose frequency, were all associated with caregiver-reported adherence. Major causes of non-adherence mentioned during the FGDs included interference of medication administration times with scheduling of routine socio-economic activities and lack of support from some non-biological caregivers. Reporting of non-adherence appeared to be hampered by perceptions of negative reactions by healthcare workers to these reports and by caregivers being unaware that the child missed some doses. Conclusions: The „new 10-day tool‟ was not shown to be superior to the „PACTG-style tool‟ in detecting non-adherence, however this new tool was found to be a valid and reliable adherence monitoring tool that included a moderately long recall period of 10 days, can be applied without the need for the respondent to remember names of individual medicines in the
- Full Text:
- Date Issued: 2014
Fishing for resilience : herbivore and algal dynamics on coral reefs in Kenya.
- Authors: Humphries, Austin Turner
- Date: 2014
- Subjects: Coral reef conservation -- Kenya , Coral reef ecology -- Kenya , Coral reef biology -- Kenya , Coral reef fishes -- Kenya , Herbivores -- Kenya , Algae -- Control -- Kenya , Fishery management -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5877 , http://hdl.handle.net/10962/d1013147
- Description: Herbivory is a key process that mediates the abundance of primary producers and community composition in both terrestrial and aquatic ecosystems. On tropical coral reefs, changes in herbivory are often related to phase shifts between coral-dominance and dominance by seaweeds, or foliose macroalgae. Resilience or capacity to resist and reverse such phase shifts is, therefore, viewed as a critical function on coral reefs. This thesis used grazer exclusion and assay experiments at six sites within three different fisheries management regimes in Kenya to identify the impacts of herbivores (sea urchins and fishes) on algal dynamics in the context of coral reef resilience. First, I examined the grazing rates necessary to prevent phase shifts by quantifying consumption and algal production. Here, I found that, over a 390-day experiment, at least 50 percent of algal production must be consumed to avoid accumulation of algal biomass. Using video observations, I also showed that scraping parrotfishes remove more algae (per unit of fish biomass) than previously assumed, and that sea urchins, if released from predation, have similar impacts to fishes. Then I focused on algal succession, and found that sea urchins and fishes have different effects that are mediated by their abundances and species composition. Where sea urchins were less abundant and parrotfishes absent (e.g. young fisheries closures), progression of algae from turfs to early and then late successional macroalgae occurred rapidly and within 100 days. I then turned my focus to the removal of already established macroalgae (grown for > 1 yr in the absence of herbivores) and showed that sea urchins and browsing fishes were able to remove significant amounts of macroalgae where either herbivore was abundant. However, using multiple-choice selectivity assays and in situ video recordings, I found that browsing fishes fed very selectively with low overlap in diet among species, leading to low functional redundancy within a high diversity system. Finally, using long-term survey data (from 28 sites) to build a 43-year chronosequence, I showed that it is possible that the effects of herbivory will not be constant across transitions from open fishing to fishery closures through non-linear grazing intensity. Therefore, increases in herbivory within fisheries closures may not be immediate and may allow a window of opportunity for algae to go from turf to unpalatable macroalgae until scraping and browsing fishes fully recover from fishing (~ 20 years). The findings in this thesis are novel and raise concern over the potential implications of the slow recovery of parrotfishes or, given lower than expected functional redundancy in grazing effects, the absence of even one browsing fish species in fisheries closures. Overall, this thesis highlights the importance of herbivore community dynamics in mediating interactions among algae, and provides new insights for conservation and management actions that attempt to bolster the resilience of coral reefs.
- Full Text:
- Date Issued: 2014
- Authors: Humphries, Austin Turner
- Date: 2014
- Subjects: Coral reef conservation -- Kenya , Coral reef ecology -- Kenya , Coral reef biology -- Kenya , Coral reef fishes -- Kenya , Herbivores -- Kenya , Algae -- Control -- Kenya , Fishery management -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5877 , http://hdl.handle.net/10962/d1013147
- Description: Herbivory is a key process that mediates the abundance of primary producers and community composition in both terrestrial and aquatic ecosystems. On tropical coral reefs, changes in herbivory are often related to phase shifts between coral-dominance and dominance by seaweeds, or foliose macroalgae. Resilience or capacity to resist and reverse such phase shifts is, therefore, viewed as a critical function on coral reefs. This thesis used grazer exclusion and assay experiments at six sites within three different fisheries management regimes in Kenya to identify the impacts of herbivores (sea urchins and fishes) on algal dynamics in the context of coral reef resilience. First, I examined the grazing rates necessary to prevent phase shifts by quantifying consumption and algal production. Here, I found that, over a 390-day experiment, at least 50 percent of algal production must be consumed to avoid accumulation of algal biomass. Using video observations, I also showed that scraping parrotfishes remove more algae (per unit of fish biomass) than previously assumed, and that sea urchins, if released from predation, have similar impacts to fishes. Then I focused on algal succession, and found that sea urchins and fishes have different effects that are mediated by their abundances and species composition. Where sea urchins were less abundant and parrotfishes absent (e.g. young fisheries closures), progression of algae from turfs to early and then late successional macroalgae occurred rapidly and within 100 days. I then turned my focus to the removal of already established macroalgae (grown for > 1 yr in the absence of herbivores) and showed that sea urchins and browsing fishes were able to remove significant amounts of macroalgae where either herbivore was abundant. However, using multiple-choice selectivity assays and in situ video recordings, I found that browsing fishes fed very selectively with low overlap in diet among species, leading to low functional redundancy within a high diversity system. Finally, using long-term survey data (from 28 sites) to build a 43-year chronosequence, I showed that it is possible that the effects of herbivory will not be constant across transitions from open fishing to fishery closures through non-linear grazing intensity. Therefore, increases in herbivory within fisheries closures may not be immediate and may allow a window of opportunity for algae to go from turf to unpalatable macroalgae until scraping and browsing fishes fully recover from fishing (~ 20 years). The findings in this thesis are novel and raise concern over the potential implications of the slow recovery of parrotfishes or, given lower than expected functional redundancy in grazing effects, the absence of even one browsing fish species in fisheries closures. Overall, this thesis highlights the importance of herbivore community dynamics in mediating interactions among algae, and provides new insights for conservation and management actions that attempt to bolster the resilience of coral reefs.
- Full Text:
- Date Issued: 2014
An investigation of parameter relationships in a high-speed digital multimedia environment
- Authors: Chigwamba, Nyasha
- Date: 2014
- Subjects: Multimedia communications , Digital communications , Local area networks (Computer networks) , Computer network architectures , Computer network protocols , Computer sound processing , Sound -- Recording and reproducing -- Digital techniques
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4725 , http://hdl.handle.net/10962/d1021153
- Description: With the rapid adoption of multimedia network technologies, a number of companies and standards bodies are introducing technologies that enhance user experience in networked multimedia environments. These technologies focus on device discovery, connection management, control, and monitoring. This study focused on control and monitoring. Multimedia networks make it possible for devices that are part of the same network to reside in different physical locations. These devices contain parameters that are used to control particular features, such as speaker volume, bass, amplifier gain, and video resolution. It is often necessary for changes in one parameter to affect other parameters, such as a synchronised change between volume and bass parameters, or collective control of multiple parameters. Thus, relationships are required between the parameters. In addition, some devices contain parameters, such as voltage, temperature, and audio level, that require constant monitoring to enable corrective action when thresholds are exceeded. Therefore, a mechanism for monitoring networked devices is required. This thesis proposes relationships that are essential for the proper functioning of a multimedia network and that should, therefore, be incorporated in standard form into a protocol, such that all devices can depend on them. Implementation mechanisms for these relationships were created. Parameter grouping and monitoring capabilities within mixing console implementations and existing control protocols were reviewed. A number of requirements for parameter grouping and monitoring were derived from this review. These requirements include a formal classification of relationship types, the ability to create relationships between parameters with different underlying value units, the ability to create relationships between parameters residing on different devices on a network, and the use of an event-driven mechanism for parameter monitoring. These requirements were the criteria used to govern the implementation mechanisms that were created as part of this study. Parameter grouping and monitoring mechanisms were implemented for the XFN protocol. The mechanisms implemented fulfil the requirements derived from the review of capabilities of mixing consoles and existing control protocols. The formal classification of relationship types was implemented within XFN parameters using lists that keep track of the relationships between each XFN parameter and other XFN parameters that reside on the same device or on other devices on the network. A common value unit, known as the global unit, was defined for use as the value format within value update messages between XFN parameters that have relationships. Mapping tables were used to translate the global unit values to application-specific (universal) units, such as decibels (dB). A mechanism for bulk parameter retrieval within the XFN protocol was augmented to produce an event-driven mechanism for parameter monitoring. These implementation mechanisms were applied to an XFN-protocol-compliant graphical control application to demonstrate their usage within an end user context. At the time of this study, the XFN protocol was undergoing standardisation within the Audio Engineering Society. The AES-64 standard has now been approved. Most of the implementation mechanisms resulting from this study have been incorporated into this standard.
- Full Text:
- Date Issued: 2014
- Authors: Chigwamba, Nyasha
- Date: 2014
- Subjects: Multimedia communications , Digital communications , Local area networks (Computer networks) , Computer network architectures , Computer network protocols , Computer sound processing , Sound -- Recording and reproducing -- Digital techniques
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4725 , http://hdl.handle.net/10962/d1021153
- Description: With the rapid adoption of multimedia network technologies, a number of companies and standards bodies are introducing technologies that enhance user experience in networked multimedia environments. These technologies focus on device discovery, connection management, control, and monitoring. This study focused on control and monitoring. Multimedia networks make it possible for devices that are part of the same network to reside in different physical locations. These devices contain parameters that are used to control particular features, such as speaker volume, bass, amplifier gain, and video resolution. It is often necessary for changes in one parameter to affect other parameters, such as a synchronised change between volume and bass parameters, or collective control of multiple parameters. Thus, relationships are required between the parameters. In addition, some devices contain parameters, such as voltage, temperature, and audio level, that require constant monitoring to enable corrective action when thresholds are exceeded. Therefore, a mechanism for monitoring networked devices is required. This thesis proposes relationships that are essential for the proper functioning of a multimedia network and that should, therefore, be incorporated in standard form into a protocol, such that all devices can depend on them. Implementation mechanisms for these relationships were created. Parameter grouping and monitoring capabilities within mixing console implementations and existing control protocols were reviewed. A number of requirements for parameter grouping and monitoring were derived from this review. These requirements include a formal classification of relationship types, the ability to create relationships between parameters with different underlying value units, the ability to create relationships between parameters residing on different devices on a network, and the use of an event-driven mechanism for parameter monitoring. These requirements were the criteria used to govern the implementation mechanisms that were created as part of this study. Parameter grouping and monitoring mechanisms were implemented for the XFN protocol. The mechanisms implemented fulfil the requirements derived from the review of capabilities of mixing consoles and existing control protocols. The formal classification of relationship types was implemented within XFN parameters using lists that keep track of the relationships between each XFN parameter and other XFN parameters that reside on the same device or on other devices on the network. A common value unit, known as the global unit, was defined for use as the value format within value update messages between XFN parameters that have relationships. Mapping tables were used to translate the global unit values to application-specific (universal) units, such as decibels (dB). A mechanism for bulk parameter retrieval within the XFN protocol was augmented to produce an event-driven mechanism for parameter monitoring. These implementation mechanisms were applied to an XFN-protocol-compliant graphical control application to demonstrate their usage within an end user context. At the time of this study, the XFN protocol was undergoing standardisation within the Audio Engineering Society. The AES-64 standard has now been approved. Most of the implementation mechanisms resulting from this study have been incorporated into this standard.
- Full Text:
- Date Issued: 2014
Political party institutionalization : a case study of Kenya
- Mutizwa-Mangiza, Shingai Price
- Authors: Mutizwa-Mangiza, Shingai Price
- Date: 2014
- Subjects: Kenya -- Politics and government , Political parties -- Kenya , Kenya -- History , Kenya -- Colonial influence
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2881 , http://hdl.handle.net/10962/d1013258
- Description: This thesis explores the nature and extent of political party institutionalization in Kenya. More specifically, it focuses on the four dimensions of party institutionalization, namely organizational systemness, value-infusion, decisional autonomy and reification. The study itself is largely located within the historical-institutionalist school of thought, with particular emphasis on the path dependency strand of this theoretical framework. However, the study also employs a political economy approach. It recognizes that the development trajectory of party politics in Kenya did not evolve in a vacuum but within a particular historical-institutional and political-economic context. The thesis advances the notion that those current low levels of party institutionalization that are evident in almost all parties, and the relatively peripheral role that they have in Kenya's governance can be traced to Kenya's colonial and post-colonial political history, the resource poor environment and the onset of globalization.
- Full Text:
- Date Issued: 2014
- Authors: Mutizwa-Mangiza, Shingai Price
- Date: 2014
- Subjects: Kenya -- Politics and government , Political parties -- Kenya , Kenya -- History , Kenya -- Colonial influence
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2881 , http://hdl.handle.net/10962/d1013258
- Description: This thesis explores the nature and extent of political party institutionalization in Kenya. More specifically, it focuses on the four dimensions of party institutionalization, namely organizational systemness, value-infusion, decisional autonomy and reification. The study itself is largely located within the historical-institutionalist school of thought, with particular emphasis on the path dependency strand of this theoretical framework. However, the study also employs a political economy approach. It recognizes that the development trajectory of party politics in Kenya did not evolve in a vacuum but within a particular historical-institutional and political-economic context. The thesis advances the notion that those current low levels of party institutionalization that are evident in almost all parties, and the relatively peripheral role that they have in Kenya's governance can be traced to Kenya's colonial and post-colonial political history, the resource poor environment and the onset of globalization.
- Full Text:
- Date Issued: 2014
Hostage incident management : preparedness and response of international non-governmental organisations
- Authors: Lauvik. Kjell Erik
- Date: 2014
- Subjects: Non-governmental organizations -- Security measures , Hostage negotiations , Conflict management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8347 , http://hdl.handle.net/10948/d1020800
- Description: It is broadly accepted that there is a need for better security management and protocols for hostage incident management, there is currently a lack of basic empirical knowledge about the existing security management protocols with reference to existing policies, knowledge and the capability of International Non-Governmental Organisations (INGOs) to handle hostage incidents. Many INGOs have successfully managed high-profile hostage crises, but there is still a considerable level of uncertainty about the way these crises have been solved and the way their success can be seen in relation to other crises. This study aimed to understand how INGOs prepare themselves for hostage incidents, whether policies, procedures are in place, how they manage hostage situations, and also how INGO staff are trained and prepared. The methodology adopted for this study was qualitative and comprised of indepth interviews with sixteen INGOs and ten industry experts and a review of INGO documents, policies and plans. The study sheds light on some of the less talked-about aspects for INGO security management in general, as well as preparedness and responsibility towards their staff. The study suggests that while most organisations have a level of preparedness in place, enhancing each agency’s respective policies may assist the organisation in better management. The study also found that there is a higher use of ransom payment than expected, and that there is an increasing willingness to engage external expertise to assisting in managing a hostage crisis. The study makes several recommendations that may have policy implications, including pre-deployment hostile environment training, reviewing potential cooperation between INGOs and United Nations, and the use of external resources to assist in managing a hostage crisis. It also recommends a revision of existing negotiation models, as the current models are lacking in addressing protracted hostage cases. The establishment of an accurate database of incidents to allow for improved interpretation of trends and scope of hostage cases is also recommended.
- Full Text:
- Date Issued: 2014
- Authors: Lauvik. Kjell Erik
- Date: 2014
- Subjects: Non-governmental organizations -- Security measures , Hostage negotiations , Conflict management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8347 , http://hdl.handle.net/10948/d1020800
- Description: It is broadly accepted that there is a need for better security management and protocols for hostage incident management, there is currently a lack of basic empirical knowledge about the existing security management protocols with reference to existing policies, knowledge and the capability of International Non-Governmental Organisations (INGOs) to handle hostage incidents. Many INGOs have successfully managed high-profile hostage crises, but there is still a considerable level of uncertainty about the way these crises have been solved and the way their success can be seen in relation to other crises. This study aimed to understand how INGOs prepare themselves for hostage incidents, whether policies, procedures are in place, how they manage hostage situations, and also how INGO staff are trained and prepared. The methodology adopted for this study was qualitative and comprised of indepth interviews with sixteen INGOs and ten industry experts and a review of INGO documents, policies and plans. The study sheds light on some of the less talked-about aspects for INGO security management in general, as well as preparedness and responsibility towards their staff. The study suggests that while most organisations have a level of preparedness in place, enhancing each agency’s respective policies may assist the organisation in better management. The study also found that there is a higher use of ransom payment than expected, and that there is an increasing willingness to engage external expertise to assisting in managing a hostage crisis. The study makes several recommendations that may have policy implications, including pre-deployment hostile environment training, reviewing potential cooperation between INGOs and United Nations, and the use of external resources to assist in managing a hostage crisis. It also recommends a revision of existing negotiation models, as the current models are lacking in addressing protracted hostage cases. The establishment of an accurate database of incidents to allow for improved interpretation of trends and scope of hostage cases is also recommended.
- Full Text:
- Date Issued: 2014
Biochemical mechanisms towards understanding Alzheimer's disease
- Authors: Padayachee, Eden Rebecca
- Date: 2014
- Subjects: Alzheimer's disease Nitric-oxide synthase Biochemical markers Amyloid beta-protein Peptide hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4103 , http://hdl.handle.net/10962/d1011092
- Description: The start of the amyloidogenic pathway in Alzheimer’s disease (AD) begins with the deposition of the Aβ₁₋₄₂ peptide surrounded by astrocytes. High levels of arginine and low amounts of neuronal nitric oxide synthase (nNOS) are associated with AD. These astrocytes store reserve arginine that is eventually metabolized by nNOS, within the vicinity of the Aβ₁₋₄₂ peptide. We propose the existence of an association vs. dissociation equilibrium between Aβ and nNOS such that nNOS is an amyloidogenic catalyst for fibrils. When Aβ binds to nNOS, it inhibits the activity of the enzyme (association phase). However when the amyloid peptide dissociates into a form that can no longer bind, later deduced as a fibril, the activity is restored. Thus, the interaction of Aβ with nNOS could serve to regulate the interaction between nNOS and arginine by restoring activity of the enzyme but at the same time promoting fibrillogenesis. Given this event occurring with the neuron, both nNOS and amyloid can serve as a biomarker for the early onset of AD. The enzyme nNOS catalyzed the formation of fibrils in the presence of Aβ peptides, while Ag nps were shown to reverse the fibril formation from Aβ peptides more so than Au and curcumin either through electrostatic or π-π stacking (aromatic) influences. Our studies have shown that the fragments of Aβ₁₋₄₂ i.e. the pentapeptide (Aβ₁₇₋₂₁) and the three glycine zipper peptides (Aβ₂₅₋₂₉, Aβ₂₉₋₃₃, Aβ₃₃₋₃₇) and the full length glycine zipper stretch (Aβ₂₅₋₃₇) all inhibited nNOS activity to varying degrees. The peptides Aβ₁₇₋₂₁ and Aβ₂₉₋₃₃ with their respective Ki values of 5.1 μM and 7.5 μM inhibited the enzyme the most. The Ki values for reversed sequenced peptides (Aβ₁₇₋₂₁r and Aβ₂₉₋₃₃r) were two fold greater than that of the original peptides while the Ki values for the polar forms (Aβ₁₇₋₂₁p and Aβ₂₉₋₃₃p) were between 3-4 fold greater than that of the original peptides. It was also found that Ag nps (Ki = 0.12 μM) inhibited the activity of nNOS the most compared to Au nps; (Ki = 0.15 μM) and curcumin (Ki = 0.25 μM). At 298K, all the ligands bound at a single site on the enzyme (n=1) and a single Trp residue (θ =1), (later identified as Trp678) was made available on the enzyme surface for quenching by the ligands. Increasing the temperature from 298K-313K, increased the value of Ksv and pointed to a dynamic quenching mechanism for Aβ peptides, nps and curcumin interaction with nNOS. The positive signs for entropy and enthalpy for all Aβ peptides nps and curcumin pointed to hydrophobic–hydrophobic interaction with the enzyme. The fact that Kd increased with temperature emphasized the endothermic nature of the binding reaction and the requirement of thermal energy to aid in diffusion of the ligand to the active site. It was concluded that the binding reaction between the ligands and nNOS was non-spontaneous and endothermic at low temperatures (+ΔG) but spontaneous at high temperatures (-ΔG). The two amino acids Tyr706 and Trp678 moved from their original positions, subject to ligand binding. Trp678 moved a minimum distance of 5 Å toward the heme while Tyr706 moved a maximum distance of 14 Å away from the heme. AutoDock 4.2 was a valuable tool in monitoring the distance of Trp678 within the enzyme interior and fluorescence resonance energy transfer (FRET) was efficient in monitoring the distance moved by Trp residues on the enzyme surface.
- Full Text:
- Date Issued: 2014
- Authors: Padayachee, Eden Rebecca
- Date: 2014
- Subjects: Alzheimer's disease Nitric-oxide synthase Biochemical markers Amyloid beta-protein Peptide hormones
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4103 , http://hdl.handle.net/10962/d1011092
- Description: The start of the amyloidogenic pathway in Alzheimer’s disease (AD) begins with the deposition of the Aβ₁₋₄₂ peptide surrounded by astrocytes. High levels of arginine and low amounts of neuronal nitric oxide synthase (nNOS) are associated with AD. These astrocytes store reserve arginine that is eventually metabolized by nNOS, within the vicinity of the Aβ₁₋₄₂ peptide. We propose the existence of an association vs. dissociation equilibrium between Aβ and nNOS such that nNOS is an amyloidogenic catalyst for fibrils. When Aβ binds to nNOS, it inhibits the activity of the enzyme (association phase). However when the amyloid peptide dissociates into a form that can no longer bind, later deduced as a fibril, the activity is restored. Thus, the interaction of Aβ with nNOS could serve to regulate the interaction between nNOS and arginine by restoring activity of the enzyme but at the same time promoting fibrillogenesis. Given this event occurring with the neuron, both nNOS and amyloid can serve as a biomarker for the early onset of AD. The enzyme nNOS catalyzed the formation of fibrils in the presence of Aβ peptides, while Ag nps were shown to reverse the fibril formation from Aβ peptides more so than Au and curcumin either through electrostatic or π-π stacking (aromatic) influences. Our studies have shown that the fragments of Aβ₁₋₄₂ i.e. the pentapeptide (Aβ₁₇₋₂₁) and the three glycine zipper peptides (Aβ₂₅₋₂₉, Aβ₂₉₋₃₃, Aβ₃₃₋₃₇) and the full length glycine zipper stretch (Aβ₂₅₋₃₇) all inhibited nNOS activity to varying degrees. The peptides Aβ₁₇₋₂₁ and Aβ₂₉₋₃₃ with their respective Ki values of 5.1 μM and 7.5 μM inhibited the enzyme the most. The Ki values for reversed sequenced peptides (Aβ₁₇₋₂₁r and Aβ₂₉₋₃₃r) were two fold greater than that of the original peptides while the Ki values for the polar forms (Aβ₁₇₋₂₁p and Aβ₂₉₋₃₃p) were between 3-4 fold greater than that of the original peptides. It was also found that Ag nps (Ki = 0.12 μM) inhibited the activity of nNOS the most compared to Au nps; (Ki = 0.15 μM) and curcumin (Ki = 0.25 μM). At 298K, all the ligands bound at a single site on the enzyme (n=1) and a single Trp residue (θ =1), (later identified as Trp678) was made available on the enzyme surface for quenching by the ligands. Increasing the temperature from 298K-313K, increased the value of Ksv and pointed to a dynamic quenching mechanism for Aβ peptides, nps and curcumin interaction with nNOS. The positive signs for entropy and enthalpy for all Aβ peptides nps and curcumin pointed to hydrophobic–hydrophobic interaction with the enzyme. The fact that Kd increased with temperature emphasized the endothermic nature of the binding reaction and the requirement of thermal energy to aid in diffusion of the ligand to the active site. It was concluded that the binding reaction between the ligands and nNOS was non-spontaneous and endothermic at low temperatures (+ΔG) but spontaneous at high temperatures (-ΔG). The two amino acids Tyr706 and Trp678 moved from their original positions, subject to ligand binding. Trp678 moved a minimum distance of 5 Å toward the heme while Tyr706 moved a maximum distance of 14 Å away from the heme. AutoDock 4.2 was a valuable tool in monitoring the distance of Trp678 within the enzyme interior and fluorescence resonance energy transfer (FRET) was efficient in monitoring the distance moved by Trp residues on the enzyme surface.
- Full Text:
- Date Issued: 2014
An integrated approach for the delineation of arable land and its cropping suitability under variable soil and climatic conditions in the Nkonkobe municipality, Eastern Cape, South Africa
- Authors: Manyevere, Alen
- Date: 2014
- Subjects: Soils -- Classification -- South Africa -- Eastern Cape , Soil degradation -- South Africa -- Eastern Cape , Ethnoscience -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Soil Science)
- Identifier: vital:11965 , http://hdl.handle.net/10353/d1019856 , Soils -- Classification -- South Africa -- Eastern Cape , Soil degradation -- South Africa -- Eastern Cape , Ethnoscience -- South Africa -- Eastern Cape
- Description: Arable crop production in Nkonkobe Municipality is low due to abandonment of potentially productive land and low productivity of the cultivated land. Little attention has been given to farmers perspectives with regards to crop production and land abandonment. Understanding the relationships of indigenous knowledge systems, where local approaches to soil classification, appraisal, use and management and land evaluation, and scientific approaches could be important for the effective use of available soil resources while avoiding those resources that are vulnerable to degradation. In addition, the interactions between soil factors and climate could be useful in understanding the erodibility of soils. The intergration of scientific research and indigenous knowledge systems could help in the identification and delineation of high potential land and on crop suitability evaluation. The objectives of the study were: (i) to determine farmers‟ perspectives with regards to land utilisation and abandonment, constraints on crop production and crop preferences, (ii) to integrate and compare indigenous knowledge systems with scientific approaches of soil classification and potential, (iii) to determine the effect of climate and soil factors on erodibilities of soils in the Municipality (iv) to delineate arable land and evaluate its suitability for maize, potato, sorghum and cowpea under rainfed agriculture. Using semi-structured and open-ended interviews, information on limitations to crop production, cropping preferences, indigenous soil classifications, cropping potential ratings and erosion was captured. Descriptive and correlation statistics were used to analyse farmers‟ responses. The information was later used for a pilot participatory mapping and the determination of the agricultural potential of the soils in three selected villages of the Municipality. Field boundaries of soil texture, colour, depth, and slope position were captured using global positioning systems (GPS). The relationship between the degree of erosion and soil and slope factors was analysed by step-wise regression. Crop suitability for rainfed agriculture was done using the FAO guidelines for Land evaluation for rainfed agriculture. The spatially referenced crop suitability classes were produced by applying the Law of Limiting Combinationusing GIS Boolean Logic. The major biophysical factors, affecting crop production and land utilisation were soil degradation and low and erratic rainfall, while other factors included lack of farming equipment and security concerns. Maize, spinach and cabbage were the main crops grown, with maize sorghum and wheat the most abandoned crops. While it was difficult to accurately correlate indigenous classification with international scientific classification, the importance of colour, texture and soil depth for both classification and soil potential, suggests that some form of correlation is possible which enabled communication and other extension information to be conveyed. The shallow and stony soil (urhete) correlated well with the Leptosols in World Reference Base (WRB) or Mispah and shallow Glenrosa soils in the South African system. The red structured clays (umhlaba obomvu) matched the Nitisols in WRB or Shortlands in the South African system. The non-swelling black clayey soils (umhlaba omnyama) matched soils with melanic A horizons in both the WRB and South African soil classification systems. The dongwe and santi soils developed in alluvial sediments belonged to the Dundee, Oakleaf or Augrabies soils in the South African classification system and fluvisols or Cambisols in the WRB system. There was good agreement between farmers assessment of the cropping potential and scientific approaches but scientifically high potential red soils were rated lowly by the farmers due to difficulties in management caused by shortages of farm machinery, especially under dryland farming. Overall, the soil factors affecting erosion were influenced largely by climate, while parent material was also important. Climate had a dominant influence on soil factors most notably fine sand and very fine sand fractions and exchangeable sodium percentage being more important on soil forms occurring in arid and semi-arid climate and less in the sub-humid and humid areas, where clay mineralogy, particularly kaolinite and sesquioxide dominated. Dolerite derived soils were the most stable and should be given the highest priority for cropping development while mudstone and shale derived soils had a lower cropping potential. While slope gradient and length had some effect on soil erosion in arid and semi arid environments its influence was generally overshadowed by soil factors especially in humid zones. Cow pea and sorghum were the most adapted crops in the region while potato and maize were marginally suitable under rainfed agriculture. The study revealed that most adapted crops were not necessarily the most preferred crops by the farmers. A small percentage of the land was delineated as arable and therefore optimisation of this available land should be prioritized.
- Full Text:
- Date Issued: 2014
- Authors: Manyevere, Alen
- Date: 2014
- Subjects: Soils -- Classification -- South Africa -- Eastern Cape , Soil degradation -- South Africa -- Eastern Cape , Ethnoscience -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Soil Science)
- Identifier: vital:11965 , http://hdl.handle.net/10353/d1019856 , Soils -- Classification -- South Africa -- Eastern Cape , Soil degradation -- South Africa -- Eastern Cape , Ethnoscience -- South Africa -- Eastern Cape
- Description: Arable crop production in Nkonkobe Municipality is low due to abandonment of potentially productive land and low productivity of the cultivated land. Little attention has been given to farmers perspectives with regards to crop production and land abandonment. Understanding the relationships of indigenous knowledge systems, where local approaches to soil classification, appraisal, use and management and land evaluation, and scientific approaches could be important for the effective use of available soil resources while avoiding those resources that are vulnerable to degradation. In addition, the interactions between soil factors and climate could be useful in understanding the erodibility of soils. The intergration of scientific research and indigenous knowledge systems could help in the identification and delineation of high potential land and on crop suitability evaluation. The objectives of the study were: (i) to determine farmers‟ perspectives with regards to land utilisation and abandonment, constraints on crop production and crop preferences, (ii) to integrate and compare indigenous knowledge systems with scientific approaches of soil classification and potential, (iii) to determine the effect of climate and soil factors on erodibilities of soils in the Municipality (iv) to delineate arable land and evaluate its suitability for maize, potato, sorghum and cowpea under rainfed agriculture. Using semi-structured and open-ended interviews, information on limitations to crop production, cropping preferences, indigenous soil classifications, cropping potential ratings and erosion was captured. Descriptive and correlation statistics were used to analyse farmers‟ responses. The information was later used for a pilot participatory mapping and the determination of the agricultural potential of the soils in three selected villages of the Municipality. Field boundaries of soil texture, colour, depth, and slope position were captured using global positioning systems (GPS). The relationship between the degree of erosion and soil and slope factors was analysed by step-wise regression. Crop suitability for rainfed agriculture was done using the FAO guidelines for Land evaluation for rainfed agriculture. The spatially referenced crop suitability classes were produced by applying the Law of Limiting Combinationusing GIS Boolean Logic. The major biophysical factors, affecting crop production and land utilisation were soil degradation and low and erratic rainfall, while other factors included lack of farming equipment and security concerns. Maize, spinach and cabbage were the main crops grown, with maize sorghum and wheat the most abandoned crops. While it was difficult to accurately correlate indigenous classification with international scientific classification, the importance of colour, texture and soil depth for both classification and soil potential, suggests that some form of correlation is possible which enabled communication and other extension information to be conveyed. The shallow and stony soil (urhete) correlated well with the Leptosols in World Reference Base (WRB) or Mispah and shallow Glenrosa soils in the South African system. The red structured clays (umhlaba obomvu) matched the Nitisols in WRB or Shortlands in the South African system. The non-swelling black clayey soils (umhlaba omnyama) matched soils with melanic A horizons in both the WRB and South African soil classification systems. The dongwe and santi soils developed in alluvial sediments belonged to the Dundee, Oakleaf or Augrabies soils in the South African classification system and fluvisols or Cambisols in the WRB system. There was good agreement between farmers assessment of the cropping potential and scientific approaches but scientifically high potential red soils were rated lowly by the farmers due to difficulties in management caused by shortages of farm machinery, especially under dryland farming. Overall, the soil factors affecting erosion were influenced largely by climate, while parent material was also important. Climate had a dominant influence on soil factors most notably fine sand and very fine sand fractions and exchangeable sodium percentage being more important on soil forms occurring in arid and semi-arid climate and less in the sub-humid and humid areas, where clay mineralogy, particularly kaolinite and sesquioxide dominated. Dolerite derived soils were the most stable and should be given the highest priority for cropping development while mudstone and shale derived soils had a lower cropping potential. While slope gradient and length had some effect on soil erosion in arid and semi arid environments its influence was generally overshadowed by soil factors especially in humid zones. Cow pea and sorghum were the most adapted crops in the region while potato and maize were marginally suitable under rainfed agriculture. The study revealed that most adapted crops were not necessarily the most preferred crops by the farmers. A small percentage of the land was delineated as arable and therefore optimisation of this available land should be prioritized.
- Full Text:
- Date Issued: 2014
Development of an ionospheric map for Africa
- Authors: Ssessanga, Nicholas
- Date: 2014
- Subjects: Ionosondes Ionosphere Ionosphere -- Observations Ionosphere -- Research -- Africa Ionospheric electron density -- Africa Ionospheric critical frequencies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5519 , http://hdl.handle.net/10962/d1011498
- Description: This thesis presents research pertaining to the development of an African Ionospheric Map (AIM). An ionospheric map is a computer program that is able to display spatial and temporal representations of ionospheric parameters such as, electron density and critical plasma frequencies, for every geographical location on the map. The purpose of this development was to make the most optimum use of all available data sources, namely ionosondes, satellites and models, and to implement error minimisation techniques in order to obtain the best result at any given location on the African continent. The focus was placed on the accurate estimation of three upper atmosphere parameters which are important for radio communications: critical frequency of the F2 layer (foF2), Total Electron Content (TEC) and the maximum usable frequency over a distance of 3000 km (M3000F2). The results show that AIM provided a more accurate estimation of the three parameters than the internationally recognised and recommended ionosphere model (IRI-2012) when used on its own. Therefore, the AIM is a more accurate solution than single independent data sources for applications requiring ionospheric mapping over the African continent.
- Full Text:
- Date Issued: 2014
- Authors: Ssessanga, Nicholas
- Date: 2014
- Subjects: Ionosondes Ionosphere Ionosphere -- Observations Ionosphere -- Research -- Africa Ionospheric electron density -- Africa Ionospheric critical frequencies
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5519 , http://hdl.handle.net/10962/d1011498
- Description: This thesis presents research pertaining to the development of an African Ionospheric Map (AIM). An ionospheric map is a computer program that is able to display spatial and temporal representations of ionospheric parameters such as, electron density and critical plasma frequencies, for every geographical location on the map. The purpose of this development was to make the most optimum use of all available data sources, namely ionosondes, satellites and models, and to implement error minimisation techniques in order to obtain the best result at any given location on the African continent. The focus was placed on the accurate estimation of three upper atmosphere parameters which are important for radio communications: critical frequency of the F2 layer (foF2), Total Electron Content (TEC) and the maximum usable frequency over a distance of 3000 km (M3000F2). The results show that AIM provided a more accurate estimation of the three parameters than the internationally recognised and recommended ionosphere model (IRI-2012) when used on its own. Therefore, the AIM is a more accurate solution than single independent data sources for applications requiring ionospheric mapping over the African continent.
- Full Text:
- Date Issued: 2014
Patterns and drivers of benthic macrofouna to support systematic conservation planning for marine unconsolidated sediment ecosystems
- Authors: Karenyi, Natasha
- Date: 2014
- Subjects: Marine sediments -- Microbiology , Biotic communities , Ecosystem management , Conservation of natural resources
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10355 , http://hdl.handle.net/10948/d1020989
- Description: Marine unconsolidated sediments constitute the largest ecosystems on earth in terms of spatial coverage, but there are still critical gaps in the science required to support conservation and ecosystem-based management. This is mainly due to the inaccessibility of these ecosystems in wave-exposed environments or deeper waters and the difficulty in observing biota in their three-dimensional sedimentary habitat. Currently, the physical driving processes of intertidal unconsolidated sediment ecosystems are much better understood than those of the subtidal ecosystems. However, these ecosystems are linked through water and sediment movement. This thesis, therefore, considers the continuum of unconsolidated sediment ecosystems across the entire continental shelf (i.e. intertidal to the shelf edge). The aim of this thesis was two-fold; (i) to advance the foundational understanding of biodiversity patterns and driving processes in unconsolidated sediment habitats, and (ii) to apply this knowledge in the development of a systematic conservation plan for marine unconsolidated sediment ecosystems. The South African west coast continental shelf was used as a case study in order to represent Eastern boundary upwelling regions. This study sought to investigate biodiversity patterns in macro-infaunal communities and determine their driving processes for incorporation into habitat classifications and the development of a habitat map. Systematic conservation plans require a map of biodiversity patterns and processes, and quantitative conservation targets to ensure representation of all biodiversity features including habitats.in marine protected areas. This thesis provided these key elements by classifying the unconsolidated sediment habitats and determining habitat-specific evidence-based conservation targets to support conservation of these important ecosystems. The application of these elements was then demonstrated in a systematic conservation plan for the unconsolidated sediment ecosystems of the South African west coast. Diversity patterns were examined using physical and macro-infauna data, ranging from the beach to the shelf edge (0-412 m). These data were analysed to develop two different habitat classifications, namely seascapes derived from geophysical and biophysical data, and biotopes derived from the combination of macro-infaunal and physical data. Multivariate analyses of 13 physical variables identified eight seascapes for the unconsolidated sediment samples from 48 sites on the South African west coast. These were based on depth, slope, sediment type, and upwelling-related processes (i.e. maximum chlorophyll concentration, sediment organic carbon content and austral summer bottom oxygen concentration). Latitude and bottom temperature were not considered major drivers of seascapes on the west coast because latitude closely reflected changes in upwelling-related processes and the temperature range was narrow across the shelf. This study revealed that productivity, a biophysical variable not usually included in geo-physical habitat classifications, played a significant role in the definition of seascapes on the South African west coast. It is therefore recommended that productivity be included in future seascape classifications to improve the utility of these classifications particularly in areas of variable productivity. Seascapes should, however, be tested against biological data to improve the understanding of key physical drivers of communities in unconsolidated sediment ecosystems. Macro-infaunal community distributions were determined along with their physical drivers for the unconsolidated sediments of the South African west coast. A total of 44 828 individuals from 469 taxa were identified from 48 sites representing 46.2 m2 of seafloor. Seven distinct macro-infaunal communities were defined through multivariate analyses and their key characteristic and distinguishing species were identified. These communities reflected five depth zones across the shelf, namely beach, inner shelf (10-42 m), middle shelf (60-142 m), outer shelf (150-357 m) and shelf edge (348-412 m). The processes driving the community structure of these depth zones were postulated to be tides, wave turbulence, seasonal hypoxia, habitat stability and homogeneity, and internal tides and/or shelf break upwelling, with drivers listed in order of increasing influence with depth. The middle shelf was further separated into three distinct communities based on sediment type, sediment organic carbon content and frequency of hypoxia. Variations in water turbulence, sediment grain size, upwelling-related variables and riverine sediment input were identified as the likely primary drivers of macro-infaunal community patterns. This chapter culminated in the development of a biotope classification based on the combination of macro-infaunal communities and their physical habitats (i.e. biotopes). South Africa has developed an expert-derived National Marine and Coastal Habitat (SANMC) Classification System which is used as a biodiversity surrogate in ecosystem assessment and spatial planning. This thesis tested the validity of this classification and the data derived Seascape classification against macro-infauna species abundance and biomass data in an effort to determine how well the different classifications represent macro-infaunal diversity of the west coast. These two classifications were also compared to the Biotope classification which combines macro-infaunal communities with their physical habitats. A canonical analysis of principle coordinates (CAP) was utilised to test the success with which each sample was allocated to the relevant habitat type in each classification. The total allocation success for each classification was used as a measure of effectiveness in terms of representing biodiversity patterns. Both classifications had similar allocation successes of 89-92 percent and 92-94 percent for the Seascape and National Habitat Classification respectively, but either over- or under-classified the macrofauna communities. The Biotope classification had the highest allocation success (98 percent), therefore it is the most accurate reflection of the macrofauna biodiversity patterns on the west coast. A key finding of this study was the increasing accuracy of classifications from physically- to expert- to biologically-derived habitat classifications. In this thesis, the Biotope classification was deemed the best representative of biodiversity patterns and was therefore used to produce the Biotope map for use in spatial assessment and planning. The distinct depth patterns that emerged in both the Seascape and Biotope classifications highlighted the need for further investigation of the relationship between depth and biodiversity. Despite variability in macro-infaunal communities, a general unifying pattern in biodiversity across the shelf was sought. Three relationships between depth and species richness have been described in the literature; namely a unimodal pattern, a positively linear relationship with depth, and no relationship between depth and species richness. These hypotheses were tested on the west coast. Two different species richness metrics were utilised to test the depth-diversity relationship, namely observed species density (spp.0.2m-2) and estimated species richness (spp.site-1). Observed species density increased from the beach to the shelf edge (350 m), then decreased to 412 m. The decline may have been due to difficulty in detecting species at greater depths as a result of sampling challenges. The inclusion of an innovative extrapolative method for estimating species richness (the capture-recapture heterogeneity model) within the Bayesian statistical framework mitigated the effects of species detection heterogeneity and revealed that species richness actually increased continuously across the shelf from beach to shelf edge. Thus the general relationship between depth and species richness is positively linear on the west coast of South Africa The new macro-infauna dataset and biotope map provided the opportunity to develop the first habitat-specific evidence-based conservation targets for unconsolidated sediments of the west coast. Species-Area Relationship (SAR) based conservation targets were developed for the biotopes using a modification of the generally accepted methodology. The accepted methodology has three steps (i) the estimation of total species richness for each habitat using the Bootstrap asymptotic estimator, (ii) the calculation of the slope of the species area curve (i.e. the z-value), and (iii) the calculation of targets representing 80 percent of the species. The inclusion of an innovative extrapolative species richness estimator, the Multi-species Site Occupancy Model (MSOM) provided better species richness estimation than the more conventional bootstrap species richness estimator, even though both are based on species accumulation. The MSOM, applied in the Bayesian statistical framework takes detectability of a species into account.
- Full Text:
- Date Issued: 2014
- Authors: Karenyi, Natasha
- Date: 2014
- Subjects: Marine sediments -- Microbiology , Biotic communities , Ecosystem management , Conservation of natural resources
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10355 , http://hdl.handle.net/10948/d1020989
- Description: Marine unconsolidated sediments constitute the largest ecosystems on earth in terms of spatial coverage, but there are still critical gaps in the science required to support conservation and ecosystem-based management. This is mainly due to the inaccessibility of these ecosystems in wave-exposed environments or deeper waters and the difficulty in observing biota in their three-dimensional sedimentary habitat. Currently, the physical driving processes of intertidal unconsolidated sediment ecosystems are much better understood than those of the subtidal ecosystems. However, these ecosystems are linked through water and sediment movement. This thesis, therefore, considers the continuum of unconsolidated sediment ecosystems across the entire continental shelf (i.e. intertidal to the shelf edge). The aim of this thesis was two-fold; (i) to advance the foundational understanding of biodiversity patterns and driving processes in unconsolidated sediment habitats, and (ii) to apply this knowledge in the development of a systematic conservation plan for marine unconsolidated sediment ecosystems. The South African west coast continental shelf was used as a case study in order to represent Eastern boundary upwelling regions. This study sought to investigate biodiversity patterns in macro-infaunal communities and determine their driving processes for incorporation into habitat classifications and the development of a habitat map. Systematic conservation plans require a map of biodiversity patterns and processes, and quantitative conservation targets to ensure representation of all biodiversity features including habitats.in marine protected areas. This thesis provided these key elements by classifying the unconsolidated sediment habitats and determining habitat-specific evidence-based conservation targets to support conservation of these important ecosystems. The application of these elements was then demonstrated in a systematic conservation plan for the unconsolidated sediment ecosystems of the South African west coast. Diversity patterns were examined using physical and macro-infauna data, ranging from the beach to the shelf edge (0-412 m). These data were analysed to develop two different habitat classifications, namely seascapes derived from geophysical and biophysical data, and biotopes derived from the combination of macro-infaunal and physical data. Multivariate analyses of 13 physical variables identified eight seascapes for the unconsolidated sediment samples from 48 sites on the South African west coast. These were based on depth, slope, sediment type, and upwelling-related processes (i.e. maximum chlorophyll concentration, sediment organic carbon content and austral summer bottom oxygen concentration). Latitude and bottom temperature were not considered major drivers of seascapes on the west coast because latitude closely reflected changes in upwelling-related processes and the temperature range was narrow across the shelf. This study revealed that productivity, a biophysical variable not usually included in geo-physical habitat classifications, played a significant role in the definition of seascapes on the South African west coast. It is therefore recommended that productivity be included in future seascape classifications to improve the utility of these classifications particularly in areas of variable productivity. Seascapes should, however, be tested against biological data to improve the understanding of key physical drivers of communities in unconsolidated sediment ecosystems. Macro-infaunal community distributions were determined along with their physical drivers for the unconsolidated sediments of the South African west coast. A total of 44 828 individuals from 469 taxa were identified from 48 sites representing 46.2 m2 of seafloor. Seven distinct macro-infaunal communities were defined through multivariate analyses and their key characteristic and distinguishing species were identified. These communities reflected five depth zones across the shelf, namely beach, inner shelf (10-42 m), middle shelf (60-142 m), outer shelf (150-357 m) and shelf edge (348-412 m). The processes driving the community structure of these depth zones were postulated to be tides, wave turbulence, seasonal hypoxia, habitat stability and homogeneity, and internal tides and/or shelf break upwelling, with drivers listed in order of increasing influence with depth. The middle shelf was further separated into three distinct communities based on sediment type, sediment organic carbon content and frequency of hypoxia. Variations in water turbulence, sediment grain size, upwelling-related variables and riverine sediment input were identified as the likely primary drivers of macro-infaunal community patterns. This chapter culminated in the development of a biotope classification based on the combination of macro-infaunal communities and their physical habitats (i.e. biotopes). South Africa has developed an expert-derived National Marine and Coastal Habitat (SANMC) Classification System which is used as a biodiversity surrogate in ecosystem assessment and spatial planning. This thesis tested the validity of this classification and the data derived Seascape classification against macro-infauna species abundance and biomass data in an effort to determine how well the different classifications represent macro-infaunal diversity of the west coast. These two classifications were also compared to the Biotope classification which combines macro-infaunal communities with their physical habitats. A canonical analysis of principle coordinates (CAP) was utilised to test the success with which each sample was allocated to the relevant habitat type in each classification. The total allocation success for each classification was used as a measure of effectiveness in terms of representing biodiversity patterns. Both classifications had similar allocation successes of 89-92 percent and 92-94 percent for the Seascape and National Habitat Classification respectively, but either over- or under-classified the macrofauna communities. The Biotope classification had the highest allocation success (98 percent), therefore it is the most accurate reflection of the macrofauna biodiversity patterns on the west coast. A key finding of this study was the increasing accuracy of classifications from physically- to expert- to biologically-derived habitat classifications. In this thesis, the Biotope classification was deemed the best representative of biodiversity patterns and was therefore used to produce the Biotope map for use in spatial assessment and planning. The distinct depth patterns that emerged in both the Seascape and Biotope classifications highlighted the need for further investigation of the relationship between depth and biodiversity. Despite variability in macro-infaunal communities, a general unifying pattern in biodiversity across the shelf was sought. Three relationships between depth and species richness have been described in the literature; namely a unimodal pattern, a positively linear relationship with depth, and no relationship between depth and species richness. These hypotheses were tested on the west coast. Two different species richness metrics were utilised to test the depth-diversity relationship, namely observed species density (spp.0.2m-2) and estimated species richness (spp.site-1). Observed species density increased from the beach to the shelf edge (350 m), then decreased to 412 m. The decline may have been due to difficulty in detecting species at greater depths as a result of sampling challenges. The inclusion of an innovative extrapolative method for estimating species richness (the capture-recapture heterogeneity model) within the Bayesian statistical framework mitigated the effects of species detection heterogeneity and revealed that species richness actually increased continuously across the shelf from beach to shelf edge. Thus the general relationship between depth and species richness is positively linear on the west coast of South Africa The new macro-infauna dataset and biotope map provided the opportunity to develop the first habitat-specific evidence-based conservation targets for unconsolidated sediments of the west coast. Species-Area Relationship (SAR) based conservation targets were developed for the biotopes using a modification of the generally accepted methodology. The accepted methodology has three steps (i) the estimation of total species richness for each habitat using the Bootstrap asymptotic estimator, (ii) the calculation of the slope of the species area curve (i.e. the z-value), and (iii) the calculation of targets representing 80 percent of the species. The inclusion of an innovative extrapolative species richness estimator, the Multi-species Site Occupancy Model (MSOM) provided better species richness estimation than the more conventional bootstrap species richness estimator, even though both are based on species accumulation. The MSOM, applied in the Bayesian statistical framework takes detectability of a species into account.
- Full Text:
- Date Issued: 2014
Evaluating the post-implementation effectiveness of selected household water treatment technologies in rural Kenya
- Authors: Onabolu, Boluwaji
- Date: 2014
- Subjects: Water-supply, Rural -- Kenya , Sanitation, Rural -- Kenya , Sanitation, Household -- Kenya , Drinking water -- Purification -- Kenya , Drinking water -- Microbiology -- Kenya , Health behavior -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6045 , http://hdl.handle.net/10962/d1013145
- Description: Water, sanitation and hygiene-related diseases are responsible for 7% of all deaths and 8% of all disability adjusted live years (DALYs), as well as the loss of 320 million days of productivity in developing countries. Though laboratory and field trials have shown that household water treatment (HWT) technologies can quickly improve the microbiological quality of drinking water, questions remain about the effectiveness of these technologies under real-world conditions. Furthermore, the value that rural communities attach to HWT is unknown, and it is not clear why, in spite of the fact that rural African households need household water treatment (HWT) most, they are the least likely to use them. The primary objective of this multi-level study was to assess the post-implementation effectiveness of selected HWT technologies in the Nyanza and Western Provinces of Kenya. The study was carried out in the rainy season between March and May, 2011 using a mixed method approach. Evidence was collected in order to build a case of evidence of HWT effectiveness or ineffectiveness in a post-implementation context. A quasi-experimental design was used first to conduct a Knowledge, Attitudes and Practices (KAP) survey in 474 households in ten intervention and five control villages (Chapter 3). The survey assessed the context in which household water treatment was being used in the study villages to provide real-world information for assessing the effectiveness of the technologies. An interviewer-administered questionnaire elicited information about the water, sanitation and hygiene-related KAP of the study communities. A household water treatment (HWT) survey (Chapter 4) was carried out in the same study households and villages as the KAP study, using a semi-structured questionnaire to gather HWT adoption, compliance and sustained use-related information to provide insight into the perceived value the study households attach to HWT technologies, and their likelihood of adoption of and compliance with these technologies. The drinking water quality of 171 (one quarter of those surveyed during KAP) randomly selected households was determined and tracked from source to the point of use (Chapter 5). This provided insights into HWT effectiveness by highlighting the need for HWT (as indicated by source water quality) and the effect of the study households’ KAP on drinking water quality (as indicated by the stored water quality). Physico-chemical and microbiological water quality of the nineteen improved and unimproved sources used by the study households was determined, according to the World Health Organisation guidelines. The microbiological quality of 291 water samples in six intervention and five control villages was determined from source to the point-of-use (POU) using the WHO and Sphere Drinking Water Quality Guidelines. An observational study design was then used to assess the post-implementation effectiveness of the technologies used in 37 households in five intervention villages (Chapter 6). Three assessments were carried out to determine the changes in the microbiological quality of 107 drinking water samples before treatment (from collection container) and after treatment (from storage container) by the households. The criteria used to assess the performance of the technologies were microbial efficacy, robustness and performance in relation to sector standards. A Quantitative Microbial Risk Assessment (QMRA) was then carried out in the HWT effectiveness study households to assess the technologies’ ability to reduce the users’ exposure to and probability of infection with water-borne pathogens (Chapter 7). The KAP survey showed that the intervention and control communities did not differ significantly in 18 out of 20 socio-economic variables that could potentially be influenced by the structured manner of introducing HWT into the intervention villages. The majority of the intervention group (IG) and the control group (CG) were poor or very poor on the basis of household assets they owned. The predominant level of education for almost two-thirds of the IG and CG respondents was primary school (completed and non-completed). Though very few were unemployed in IG (8.07%) and CG (14.29%), the two groups of respondents were predominantly engaged in subsistence farming — a low income occupation. With regard to practices, both groups had inadequate access to water and sanitation with only one in two of the households in both IG and CG using improved water sources as their main drinking water source in the non-rainy season. One in ten households in both study groups possessed an improved sanitation facility, though the CG was significantly more likely to practice open defecation than the IG. The self-reported use of soap in both study groups was mainly for bathing and not for handwashing after faecal contact with adult or child faeces. Despite the study groups' knowledge about diarrhoea, both groups showed a disconnection between their knowledge about routes of contamination and barriers to contamination. The most frequent reason for not treating water was the perceived safety of rain water in both the IG and CG. , The HWT adoption survey revealed poor storage and water-handling practices in both IG and CG, and that very few respondents knew how to use the HWT technologies correctly: The IG and CG were similar in perceived value attached to household water treatment. All HWT technologies had a lower likelihood of adoption compared to the likelihood of compliance indicators in both IG and CG. The users’ perceptions about efficacy, time taken and ease of use of the HWT technologies lowered the perceived value attached to the technologies. The assessment of the drinking water quality used by the study communities indicated that the improved sources had a lower geometric mean E. coli and total coliform count than the unimproved sources. Both categories of sources were of poor microbiological quality and both exceeded the Sphere Project (2004) and the WHO (2008) guidelines for total coliforms and E. Coli respectively The study communities’ predominant drinking water sources, surface water and rainwater were faecally contaminated (geometric mean E. coli load of 388.1±30.45 and 38.9±22.35 cfu/100 ml respectively) and needed effective HWT. The improved sources were significantly more likely than the unimproved sources to have a higher proportion of samples that complied with the WHO drinking water guidelines at source, highlighting the importance of providing improved water sources. The lowest levels of faecal contamination were observed between the collection and storage points which coincided with the stage at which HWT is normally applied, suggesting an HWT effect on the water quality. All water sources had nitrate and turbidity levels that exceeded the WHO stipulated guidelines, while some of the improved and unimproved sources had higher than permissible levels of lead, manganese and aluminium. The water source category and the mouth type of the storage container were predictive of the stored water quality. The active treater households had a higher percentage of samples that complied with WHO water quality guidelines for E. coli than inactive treater households in both improved and unimproved source categories. In inactive treater households, 65% of storage container water samples from the improved sources complied with the WHO guidelines in comparison to 72% of the stored water samples in the active treater households. However the differences were not statistically significant. The HWT technologies did not attain sector standards of effective performance: in descending order, the mean log10 reduction in E. coli concentrations after treatment of water from unimproved sources was PUR (log₁₀ 2.0), ceramic filters (log₁₀ 1.57), Aquatab (log₁₀ 1.06) and Waterguard (log₁₀ 0.44). The mean log10 reduction in E. coli after treatment of water from improved sources was Aquatab (log₁₀ 2.3), Waterguard (log₁₀ 1.43), PUR (log₁₀ 0.94) and ceramic filters (log₁₀ 0.16). The HWT technologies reduced the user’s daily exposure to water-borne pathogens from both unimproved and improved drinking water sources. The mean difference in exposure after treatment of water from unimproved sources was ceramic filter (log₁₀ 2.1), Aquatab (log₁₀ 1.9), PUR (log₁₀ 1.5) and Waterguard (log₁₀ 0.9), in descending order. The mean probability of infection with water-borne pathogens (using E.coli as indicator) after consumption of treated water from both improved and unimproved sources was reduced in users of all the HWT technologies. The difference in reduction between technologies was not statistically significant. The study concluded that despite the apparent need for HWT, the study households’ inadequate knowledge, poor attitudes and unhygienic practices make it unlikely that they will use the technologies effectively to reduce microbial concentrations to the standards stipulated by accepted drinking water quality guidelines. The structured method of HWT promotion in the intervention villages had not resulted in more hygienic water and sanitation KAP in the IG compared to the CG, or significant differences in likelihood of adoption and compliance with the assessed HWT technologies. Despite attaching a high perceived value to HWT, insufficient knowledge about how to use the HWT technologies and user concerns about factors such as ease of use, accessibility and time to use will impact negatively on adoption and compliance with HWT, notwithstanding their efficacy during field trials. Even though external support had been withdrawn, the assessed HWT technologies were able improve the quality of household drinking water and reduce the exposure and risk of water-borne infections. However, the improvement in water quality and reduction in risk did not attain sector guidelines, highlighting the need to address the attitudes, practices and design criteria identified in this study which limit the adoption, compliance and effective use of these technologies. These findings have implications for HWT interventions, emphasising the need for practice-based behavioural support alongside technical support.
- Full Text:
- Date Issued: 2014
- Authors: Onabolu, Boluwaji
- Date: 2014
- Subjects: Water-supply, Rural -- Kenya , Sanitation, Rural -- Kenya , Sanitation, Household -- Kenya , Drinking water -- Purification -- Kenya , Drinking water -- Microbiology -- Kenya , Health behavior -- Kenya
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:6045 , http://hdl.handle.net/10962/d1013145
- Description: Water, sanitation and hygiene-related diseases are responsible for 7% of all deaths and 8% of all disability adjusted live years (DALYs), as well as the loss of 320 million days of productivity in developing countries. Though laboratory and field trials have shown that household water treatment (HWT) technologies can quickly improve the microbiological quality of drinking water, questions remain about the effectiveness of these technologies under real-world conditions. Furthermore, the value that rural communities attach to HWT is unknown, and it is not clear why, in spite of the fact that rural African households need household water treatment (HWT) most, they are the least likely to use them. The primary objective of this multi-level study was to assess the post-implementation effectiveness of selected HWT technologies in the Nyanza and Western Provinces of Kenya. The study was carried out in the rainy season between March and May, 2011 using a mixed method approach. Evidence was collected in order to build a case of evidence of HWT effectiveness or ineffectiveness in a post-implementation context. A quasi-experimental design was used first to conduct a Knowledge, Attitudes and Practices (KAP) survey in 474 households in ten intervention and five control villages (Chapter 3). The survey assessed the context in which household water treatment was being used in the study villages to provide real-world information for assessing the effectiveness of the technologies. An interviewer-administered questionnaire elicited information about the water, sanitation and hygiene-related KAP of the study communities. A household water treatment (HWT) survey (Chapter 4) was carried out in the same study households and villages as the KAP study, using a semi-structured questionnaire to gather HWT adoption, compliance and sustained use-related information to provide insight into the perceived value the study households attach to HWT technologies, and their likelihood of adoption of and compliance with these technologies. The drinking water quality of 171 (one quarter of those surveyed during KAP) randomly selected households was determined and tracked from source to the point of use (Chapter 5). This provided insights into HWT effectiveness by highlighting the need for HWT (as indicated by source water quality) and the effect of the study households’ KAP on drinking water quality (as indicated by the stored water quality). Physico-chemical and microbiological water quality of the nineteen improved and unimproved sources used by the study households was determined, according to the World Health Organisation guidelines. The microbiological quality of 291 water samples in six intervention and five control villages was determined from source to the point-of-use (POU) using the WHO and Sphere Drinking Water Quality Guidelines. An observational study design was then used to assess the post-implementation effectiveness of the technologies used in 37 households in five intervention villages (Chapter 6). Three assessments were carried out to determine the changes in the microbiological quality of 107 drinking water samples before treatment (from collection container) and after treatment (from storage container) by the households. The criteria used to assess the performance of the technologies were microbial efficacy, robustness and performance in relation to sector standards. A Quantitative Microbial Risk Assessment (QMRA) was then carried out in the HWT effectiveness study households to assess the technologies’ ability to reduce the users’ exposure to and probability of infection with water-borne pathogens (Chapter 7). The KAP survey showed that the intervention and control communities did not differ significantly in 18 out of 20 socio-economic variables that could potentially be influenced by the structured manner of introducing HWT into the intervention villages. The majority of the intervention group (IG) and the control group (CG) were poor or very poor on the basis of household assets they owned. The predominant level of education for almost two-thirds of the IG and CG respondents was primary school (completed and non-completed). Though very few were unemployed in IG (8.07%) and CG (14.29%), the two groups of respondents were predominantly engaged in subsistence farming — a low income occupation. With regard to practices, both groups had inadequate access to water and sanitation with only one in two of the households in both IG and CG using improved water sources as their main drinking water source in the non-rainy season. One in ten households in both study groups possessed an improved sanitation facility, though the CG was significantly more likely to practice open defecation than the IG. The self-reported use of soap in both study groups was mainly for bathing and not for handwashing after faecal contact with adult or child faeces. Despite the study groups' knowledge about diarrhoea, both groups showed a disconnection between their knowledge about routes of contamination and barriers to contamination. The most frequent reason for not treating water was the perceived safety of rain water in both the IG and CG. , The HWT adoption survey revealed poor storage and water-handling practices in both IG and CG, and that very few respondents knew how to use the HWT technologies correctly: The IG and CG were similar in perceived value attached to household water treatment. All HWT technologies had a lower likelihood of adoption compared to the likelihood of compliance indicators in both IG and CG. The users’ perceptions about efficacy, time taken and ease of use of the HWT technologies lowered the perceived value attached to the technologies. The assessment of the drinking water quality used by the study communities indicated that the improved sources had a lower geometric mean E. coli and total coliform count than the unimproved sources. Both categories of sources were of poor microbiological quality and both exceeded the Sphere Project (2004) and the WHO (2008) guidelines for total coliforms and E. Coli respectively The study communities’ predominant drinking water sources, surface water and rainwater were faecally contaminated (geometric mean E. coli load of 388.1±30.45 and 38.9±22.35 cfu/100 ml respectively) and needed effective HWT. The improved sources were significantly more likely than the unimproved sources to have a higher proportion of samples that complied with the WHO drinking water guidelines at source, highlighting the importance of providing improved water sources. The lowest levels of faecal contamination were observed between the collection and storage points which coincided with the stage at which HWT is normally applied, suggesting an HWT effect on the water quality. All water sources had nitrate and turbidity levels that exceeded the WHO stipulated guidelines, while some of the improved and unimproved sources had higher than permissible levels of lead, manganese and aluminium. The water source category and the mouth type of the storage container were predictive of the stored water quality. The active treater households had a higher percentage of samples that complied with WHO water quality guidelines for E. coli than inactive treater households in both improved and unimproved source categories. In inactive treater households, 65% of storage container water samples from the improved sources complied with the WHO guidelines in comparison to 72% of the stored water samples in the active treater households. However the differences were not statistically significant. The HWT technologies did not attain sector standards of effective performance: in descending order, the mean log10 reduction in E. coli concentrations after treatment of water from unimproved sources was PUR (log₁₀ 2.0), ceramic filters (log₁₀ 1.57), Aquatab (log₁₀ 1.06) and Waterguard (log₁₀ 0.44). The mean log10 reduction in E. coli after treatment of water from improved sources was Aquatab (log₁₀ 2.3), Waterguard (log₁₀ 1.43), PUR (log₁₀ 0.94) and ceramic filters (log₁₀ 0.16). The HWT technologies reduced the user’s daily exposure to water-borne pathogens from both unimproved and improved drinking water sources. The mean difference in exposure after treatment of water from unimproved sources was ceramic filter (log₁₀ 2.1), Aquatab (log₁₀ 1.9), PUR (log₁₀ 1.5) and Waterguard (log₁₀ 0.9), in descending order. The mean probability of infection with water-borne pathogens (using E.coli as indicator) after consumption of treated water from both improved and unimproved sources was reduced in users of all the HWT technologies. The difference in reduction between technologies was not statistically significant. The study concluded that despite the apparent need for HWT, the study households’ inadequate knowledge, poor attitudes and unhygienic practices make it unlikely that they will use the technologies effectively to reduce microbial concentrations to the standards stipulated by accepted drinking water quality guidelines. The structured method of HWT promotion in the intervention villages had not resulted in more hygienic water and sanitation KAP in the IG compared to the CG, or significant differences in likelihood of adoption and compliance with the assessed HWT technologies. Despite attaching a high perceived value to HWT, insufficient knowledge about how to use the HWT technologies and user concerns about factors such as ease of use, accessibility and time to use will impact negatively on adoption and compliance with HWT, notwithstanding their efficacy during field trials. Even though external support had been withdrawn, the assessed HWT technologies were able improve the quality of household drinking water and reduce the exposure and risk of water-borne infections. However, the improvement in water quality and reduction in risk did not attain sector guidelines, highlighting the need to address the attitudes, practices and design criteria identified in this study which limit the adoption, compliance and effective use of these technologies. These findings have implications for HWT interventions, emphasising the need for practice-based behavioural support alongside technical support.
- Full Text:
- Date Issued: 2014
A case study of a pre-service mathematics education course to grow and develop proficient teaching in mathematics in the intermediate phase
- Authors: Lee, Amanda Jane
- Date: 2014
- Subjects: Mathematics -- Study and teaching -- South Africa , Mathematics teachers -- Training of -- South Africa , Mathematics -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2004 , http://hdl.handle.net/10962/d1015664
- Description: This research study investigated the ways in which a mathematics module, informed by an enactivist philosophy, enabled pre-service teachers to unpack the reality of their teaching practice in terms of proficient teaching. Given the generally poor mathematics results in South Africa it is not enough for teachers to be merely proficient in Mathematics. They also need to be in a position to explain important mathematical concepts to children in a manner that will encourage and develop an understanding of the basic mathematical concepts. It was my intention with this study to determine whether a mathematics education module, that embraced the underlying themes of enactivism as part of its teaching pedagogy, could have the potential to develop and increase the skills of pre-service teachers’ teaching for proficiency in Mathematics. The mathematics module was underpinned by five themes of enactivism namely: autonomy, embodiment, emergence, sense-making and experience and was designed to supplement the pre-service teachers’ basic skills in Mathematics in the Intermediate Phase. This mathematics module was offered to fourth year pre-service teachers completing a B.Ed. in the Foundation Phase at a private institute specialising in the training of teachers. The theoretical framework was informed by enactivism and how the themes of enactivism could be used as a vehicle to develop teaching proficiency. The study was qualitative in nature and situated within an interpretivist paradigm. The specific perspectives of interpretivism that were used were hermeneutics, phenomenology and reflexivity. The research design was a case study that contained elements of action research and encompassed three phases of data collection. The first phase focused on the pre-service teachers’ approach to teaching Mathematics and what this brought forth in terms of the reality of their teaching practice and the problems they encountered. The second phase undertook to determine what growth and development of teaching proficiency in Mathematics had emerged over the research period. The final phase was undertaken after the pre-service teachers had graduated and were employed as full time teachers in the Intermediate Phase. The analytical framework and lens through which the data was analysed was that of Kilpatrick, Swafford and Findell’s (2001) strands of mathematical proficiency. The argument that I present is that the themes of enactivism did contribute to the growth of the pre-service teachers’ teaching for mathematical proficiency. The themes of embodiment and experience were major contributions in revealing that this was a reality for the pre-service teachers from a practical perspective and was what they would be able to take away with them. However the theme of emergence stood out as the principle that generated the most awareness and growth and which, in turn, affected the participants’ autonomy.
- Full Text:
- Date Issued: 2014
- Authors: Lee, Amanda Jane
- Date: 2014
- Subjects: Mathematics -- Study and teaching -- South Africa , Mathematics teachers -- Training of -- South Africa , Mathematics -- Study and teaching (Elementary) -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2004 , http://hdl.handle.net/10962/d1015664
- Description: This research study investigated the ways in which a mathematics module, informed by an enactivist philosophy, enabled pre-service teachers to unpack the reality of their teaching practice in terms of proficient teaching. Given the generally poor mathematics results in South Africa it is not enough for teachers to be merely proficient in Mathematics. They also need to be in a position to explain important mathematical concepts to children in a manner that will encourage and develop an understanding of the basic mathematical concepts. It was my intention with this study to determine whether a mathematics education module, that embraced the underlying themes of enactivism as part of its teaching pedagogy, could have the potential to develop and increase the skills of pre-service teachers’ teaching for proficiency in Mathematics. The mathematics module was underpinned by five themes of enactivism namely: autonomy, embodiment, emergence, sense-making and experience and was designed to supplement the pre-service teachers’ basic skills in Mathematics in the Intermediate Phase. This mathematics module was offered to fourth year pre-service teachers completing a B.Ed. in the Foundation Phase at a private institute specialising in the training of teachers. The theoretical framework was informed by enactivism and how the themes of enactivism could be used as a vehicle to develop teaching proficiency. The study was qualitative in nature and situated within an interpretivist paradigm. The specific perspectives of interpretivism that were used were hermeneutics, phenomenology and reflexivity. The research design was a case study that contained elements of action research and encompassed three phases of data collection. The first phase focused on the pre-service teachers’ approach to teaching Mathematics and what this brought forth in terms of the reality of their teaching practice and the problems they encountered. The second phase undertook to determine what growth and development of teaching proficiency in Mathematics had emerged over the research period. The final phase was undertaken after the pre-service teachers had graduated and were employed as full time teachers in the Intermediate Phase. The analytical framework and lens through which the data was analysed was that of Kilpatrick, Swafford and Findell’s (2001) strands of mathematical proficiency. The argument that I present is that the themes of enactivism did contribute to the growth of the pre-service teachers’ teaching for mathematical proficiency. The themes of embodiment and experience were major contributions in revealing that this was a reality for the pre-service teachers from a practical perspective and was what they would be able to take away with them. However the theme of emergence stood out as the principle that generated the most awareness and growth and which, in turn, affected the participants’ autonomy.
- Full Text:
- Date Issued: 2014
The characterization of DNAJC3: elucidating the function of the TPR domains
- Mutsvunguma, Lorraine Zvichapera
- Authors: Mutsvunguma, Lorraine Zvichapera
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/55874 , vital:26751
- Description: DNAJC3 is a novel member of the DNAJ family with two domains linked to co-chaperone functions, namely the tetratricopeptide repeat (TPR) and J domain. Out of the two domains, the TPR domains are the least characterized. Therefore, the aim of this study was to characterize and elucidate additional functions of DNAJC3 TPR domains through in silico, in vitro and ex vivo approaches. Through multiple sequence and structural alignment as well as electrostatic potential analysis, DNAJC3 TPR domain were found to be most similar to TPR-containing proteins with Hsp90 or Hsp70 independent functions. In vitro pull down assays illustrated that DNAJC3 TPR domains did not interact with either cytosolic Hsp90 and Hsp70 or Grp78 and Grp94 directly, however a potential indirect interaction with Grp94 and Hsp90 was observed in mammalian lysates, via pull down assays; suggesting the formation of a complex between the proteins mediated by a specific substrate. DNAJC3 TPR domains were found to bind indiscriminately to both native and heat denatured substrates in a dose dependent manner. DNAJC3 TPR domains bound to β-galactosidase with greater affinity than malate dehydrogenase (MDH), suggesting that DNAJC3 TPR domains might exhibit substrate specificity that has not been reported before. Preliminary ex vivo analysis of DNAJC3 in mammalian cells showed that induced stress conditions did not alter the cytosolic or endoplasmic reticulum (ER) localization, or levels of DNAJC3 protein, suggesting that the protein is not stress inducible. However, protein levels of DNAJC3 were dramatically reduced by Hsp90 inhibitor novobiocin at 500 μM. Transient knockdown DNAJC3 did not change the protein levels of either Grp78 or Grp94, but decreased the protein levels of Hsp70/Hsp90 organizing protein HOP. On the other hand, protein levels of DNAJC3 were increased in HOP depleted cells. In conclusion, this study was the first to experimentally demonstrate that DNAJC3 TPR domains do not interact directly with Hsp90, Hsp70, Grp78 or Grp94, and therefore DNAJC3 is unlikely to participate in traditional co-chaperone interactions with those proteins via its TPR domain. However, the J domain is known to interact with Grp78. The discovery that DNAJC3 TPR domains resemble that of TPR-containing proteins with functions independent of Hsp90 or Hsp70 suggests that DNAJC3 might link the Hsp70/Grp78 chaperone machinery to non co-chaperone related functions, which requires further analysis.
- Full Text:
- Date Issued: 2014
- Authors: Mutsvunguma, Lorraine Zvichapera
- Date: 2014
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/55874 , vital:26751
- Description: DNAJC3 is a novel member of the DNAJ family with two domains linked to co-chaperone functions, namely the tetratricopeptide repeat (TPR) and J domain. Out of the two domains, the TPR domains are the least characterized. Therefore, the aim of this study was to characterize and elucidate additional functions of DNAJC3 TPR domains through in silico, in vitro and ex vivo approaches. Through multiple sequence and structural alignment as well as electrostatic potential analysis, DNAJC3 TPR domain were found to be most similar to TPR-containing proteins with Hsp90 or Hsp70 independent functions. In vitro pull down assays illustrated that DNAJC3 TPR domains did not interact with either cytosolic Hsp90 and Hsp70 or Grp78 and Grp94 directly, however a potential indirect interaction with Grp94 and Hsp90 was observed in mammalian lysates, via pull down assays; suggesting the formation of a complex between the proteins mediated by a specific substrate. DNAJC3 TPR domains were found to bind indiscriminately to both native and heat denatured substrates in a dose dependent manner. DNAJC3 TPR domains bound to β-galactosidase with greater affinity than malate dehydrogenase (MDH), suggesting that DNAJC3 TPR domains might exhibit substrate specificity that has not been reported before. Preliminary ex vivo analysis of DNAJC3 in mammalian cells showed that induced stress conditions did not alter the cytosolic or endoplasmic reticulum (ER) localization, or levels of DNAJC3 protein, suggesting that the protein is not stress inducible. However, protein levels of DNAJC3 were dramatically reduced by Hsp90 inhibitor novobiocin at 500 μM. Transient knockdown DNAJC3 did not change the protein levels of either Grp78 or Grp94, but decreased the protein levels of Hsp70/Hsp90 organizing protein HOP. On the other hand, protein levels of DNAJC3 were increased in HOP depleted cells. In conclusion, this study was the first to experimentally demonstrate that DNAJC3 TPR domains do not interact directly with Hsp90, Hsp70, Grp78 or Grp94, and therefore DNAJC3 is unlikely to participate in traditional co-chaperone interactions with those proteins via its TPR domain. However, the J domain is known to interact with Grp78. The discovery that DNAJC3 TPR domains resemble that of TPR-containing proteins with functions independent of Hsp90 or Hsp70 suggests that DNAJC3 might link the Hsp70/Grp78 chaperone machinery to non co-chaperone related functions, which requires further analysis.
- Full Text:
- Date Issued: 2014
The development of a personal philosophy and practice of servant leadership : a grounded theory study
- Authors: Taylor, Simon Michael
- Date: 2014
- Subjects: Hilton College (Pietermaritzburg, South Africa) , Servant leadership , Grounded theory , Community and school -- South Africa -- KwaZulu-Natal , Student volunteers in social service -- South Africa -- KwaZulu-Natal , Educational leadership -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1199 , http://hdl.handle.net/10962/d1012986
- Description: The purpose of this study is to develop a substantive grounded theory explaining the development of a philosophy and the practice of leadership amongst young adults who had attended Hilton College and whom were exposed to their servant leadership development programme. The grounded theory method in this study was developed using conventions identified by Strauss and Corbin (1990) and relying upon a collection of incidents noted during interviews with former students, teachers, housemasters, headmaster and Hiltonian Society board members. In total thirty-six interviews were conducted over a period of four years in South Africa, the United Kingdom and Kenya. Using the grounded theory methodology, an understanding of the theoretical model emerged through the development of a personal philosophy and the practice of servant leadership. Related to the central phenomenon of individual leadership philosophy and practice, the causal condition of opportunity to lead, influenced how the individual philosophy and practice emerged. Strategies used by the participants to nurture their philosophy and practice of leadership were the leadership development programme, community service, feedback and reflection. The data identified the intervening conditions and conditions relating to the context of the leadership philosophy and practice. The consequences of developing a leadership philosophy and practice were related to leadership behaviour; self-esteem; growth; follower relations; empowering of others; and relationship to institutions. The theoretical model illustrated the holistic nature of an individual’s leadership philosophy and practice. In this instance, the nature of the data revealed that the individual's leadership philosophy and practice that developed amongst the participants was predominately servant leadership. The different approaches to leadership development were scrutinised with the intention of locating the grounded theory that developed in this study, within the available literature. The literature did provide some useful insights, in particular the social field theory of Bourdieu (1998), which offered a more encompassing explanation and showed much promise in providing an understanding of leadership development. Wheatley's (1999) interpretation of field theory further explained the influence of servant leadership in leadership development. Finally, the researcher developed a set of propositions and recommendations for practice and future research and discussed the value of this research.
- Full Text:
- Date Issued: 2014
- Authors: Taylor, Simon Michael
- Date: 2014
- Subjects: Hilton College (Pietermaritzburg, South Africa) , Servant leadership , Grounded theory , Community and school -- South Africa -- KwaZulu-Natal , Student volunteers in social service -- South Africa -- KwaZulu-Natal , Educational leadership -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1199 , http://hdl.handle.net/10962/d1012986
- Description: The purpose of this study is to develop a substantive grounded theory explaining the development of a philosophy and the practice of leadership amongst young adults who had attended Hilton College and whom were exposed to their servant leadership development programme. The grounded theory method in this study was developed using conventions identified by Strauss and Corbin (1990) and relying upon a collection of incidents noted during interviews with former students, teachers, housemasters, headmaster and Hiltonian Society board members. In total thirty-six interviews were conducted over a period of four years in South Africa, the United Kingdom and Kenya. Using the grounded theory methodology, an understanding of the theoretical model emerged through the development of a personal philosophy and the practice of servant leadership. Related to the central phenomenon of individual leadership philosophy and practice, the causal condition of opportunity to lead, influenced how the individual philosophy and practice emerged. Strategies used by the participants to nurture their philosophy and practice of leadership were the leadership development programme, community service, feedback and reflection. The data identified the intervening conditions and conditions relating to the context of the leadership philosophy and practice. The consequences of developing a leadership philosophy and practice were related to leadership behaviour; self-esteem; growth; follower relations; empowering of others; and relationship to institutions. The theoretical model illustrated the holistic nature of an individual’s leadership philosophy and practice. In this instance, the nature of the data revealed that the individual's leadership philosophy and practice that developed amongst the participants was predominately servant leadership. The different approaches to leadership development were scrutinised with the intention of locating the grounded theory that developed in this study, within the available literature. The literature did provide some useful insights, in particular the social field theory of Bourdieu (1998), which offered a more encompassing explanation and showed much promise in providing an understanding of leadership development. Wheatley's (1999) interpretation of field theory further explained the influence of servant leadership in leadership development. Finally, the researcher developed a set of propositions and recommendations for practice and future research and discussed the value of this research.
- Full Text:
- Date Issued: 2014
Efficacy of two medical plant extracts and metformin in the prevention of diet induced fatty liver
- Tshidino, Shonisani Cathphonia
- Authors: Tshidino, Shonisani Cathphonia
- Date: 2014
- Subjects: Plant Extracts -- Therapeutic use , Medicinal plants
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/9066 , vital:26461
- Description: Non‐alcoholic fatty liver diseases (NAFLD) is manifested in the absent of alcohol abuse. This disease is the major cause of liver failure and death among adults and children worldwide, including South Africa. Its increasing prevalence urges the need of therapeutic intervention. The main objectives of this study were to investigate the following: (1) The effect of 38.9% high fat diet (HFD)‐induced insulin resistance and fatty liver in male Wistar rats, (2) The efficacy of aqueous extracts from Sutherlandia frutescens leaves and Prunus africana bark and metformin in the treatment of HFDinduced insulin resistance and fatty liver. Male Wistar rats were fed on HFD (the HF group) or normal rat chow (the LF group) for 12 weeks. Even though the HFD‐fed rats had developed insulin resistance by week 12, fatty liver developed by week 16. After week 12, the HF group was divided into four groups of 6‐7 rats each and three of those groups were gavaged with either 0.125 mg P. africana extract/kg bwt/day (the HF+Pa group) or 50 mg S. frutescens extract kg bwt/day (the HF+Sf group) or 16 mg metformin/ kg bwt/day (HF+Met group), while kept on the same diet for an additional of 4 weeks, to investigate whether two medicinal plant extracts and metformin can prevent HFD to induce fatty liver or not. After 16 weeks, the liver histological images revealed that the HF group developed fatty liver in the form of both microsteatosis and macrosteatosis. Fatty liver was confirmed by significant increased liver total lipid (TL) and activities of glucose‐6‐phosphate dehydrogenase (cG6PD) and xanthine oxidase (XO), mitochondrial NADH oxidase (mNOX) and by a decrease (P<0.05) in the activities of the homogenate superoxide dismutase (hSOD) and mitochondrial complex II in the HF group, when compared to the LF group. Since the activities of mCS and cACL enzymes were not changed in the HF group, hence increased cG6PD activity in the HF group indicates that there was increased NADPH demand for lipid accumulation from activated NEFAs taken up by the liver from circulation and for maintenance of the NADPH‐dependent antioxidants and oxidants, respectively. The obtained data also show that mitochondria of the HFD‐fed rats adapted to an increase in energy availability, thereby compensation through decreasing complex II activity, to allow electron flux from β‐oxidation to respiratory chain in the HF group. Liver TL content was significantly decreased in the rats treated with metformin and P. africana extract, but not in the rats treated with S. frutescens when compared to the HF group (P < 0.05). However, the TL content remained >5% per liver weight in all treated groups. The present study demonstrates that these two plant extracts and metformin have different glucogenic and lipogenic effects from that presented by HFD alone when compared to the LFD alone. In conclusion, metformin and P. africana extract can attenuate HFD‐induced fatty liver without changing the dietary habits. Hence S. frutescens extract is less effective in the prevention of HFD‐induced fatty liver. A change in the dietary habits is recommended to be considered during the use of these three remedies in the treatment of HFD‐induced insulin resistance and fatty liver. All three treatments enhanced antioxidant capacity, and may improve insulin resistance and fatty liver mediated by the present HFD through different mechanism of actions in the liver.
- Full Text:
- Date Issued: 2014
- Authors: Tshidino, Shonisani Cathphonia
- Date: 2014
- Subjects: Plant Extracts -- Therapeutic use , Medicinal plants
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/9066 , vital:26461
- Description: Non‐alcoholic fatty liver diseases (NAFLD) is manifested in the absent of alcohol abuse. This disease is the major cause of liver failure and death among adults and children worldwide, including South Africa. Its increasing prevalence urges the need of therapeutic intervention. The main objectives of this study were to investigate the following: (1) The effect of 38.9% high fat diet (HFD)‐induced insulin resistance and fatty liver in male Wistar rats, (2) The efficacy of aqueous extracts from Sutherlandia frutescens leaves and Prunus africana bark and metformin in the treatment of HFDinduced insulin resistance and fatty liver. Male Wistar rats were fed on HFD (the HF group) or normal rat chow (the LF group) for 12 weeks. Even though the HFD‐fed rats had developed insulin resistance by week 12, fatty liver developed by week 16. After week 12, the HF group was divided into four groups of 6‐7 rats each and three of those groups were gavaged with either 0.125 mg P. africana extract/kg bwt/day (the HF+Pa group) or 50 mg S. frutescens extract kg bwt/day (the HF+Sf group) or 16 mg metformin/ kg bwt/day (HF+Met group), while kept on the same diet for an additional of 4 weeks, to investigate whether two medicinal plant extracts and metformin can prevent HFD to induce fatty liver or not. After 16 weeks, the liver histological images revealed that the HF group developed fatty liver in the form of both microsteatosis and macrosteatosis. Fatty liver was confirmed by significant increased liver total lipid (TL) and activities of glucose‐6‐phosphate dehydrogenase (cG6PD) and xanthine oxidase (XO), mitochondrial NADH oxidase (mNOX) and by a decrease (P<0.05) in the activities of the homogenate superoxide dismutase (hSOD) and mitochondrial complex II in the HF group, when compared to the LF group. Since the activities of mCS and cACL enzymes were not changed in the HF group, hence increased cG6PD activity in the HF group indicates that there was increased NADPH demand for lipid accumulation from activated NEFAs taken up by the liver from circulation and for maintenance of the NADPH‐dependent antioxidants and oxidants, respectively. The obtained data also show that mitochondria of the HFD‐fed rats adapted to an increase in energy availability, thereby compensation through decreasing complex II activity, to allow electron flux from β‐oxidation to respiratory chain in the HF group. Liver TL content was significantly decreased in the rats treated with metformin and P. africana extract, but not in the rats treated with S. frutescens when compared to the HF group (P < 0.05). However, the TL content remained >5% per liver weight in all treated groups. The present study demonstrates that these two plant extracts and metformin have different glucogenic and lipogenic effects from that presented by HFD alone when compared to the LFD alone. In conclusion, metformin and P. africana extract can attenuate HFD‐induced fatty liver without changing the dietary habits. Hence S. frutescens extract is less effective in the prevention of HFD‐induced fatty liver. A change in the dietary habits is recommended to be considered during the use of these three remedies in the treatment of HFD‐induced insulin resistance and fatty liver. All three treatments enhanced antioxidant capacity, and may improve insulin resistance and fatty liver mediated by the present HFD through different mechanism of actions in the liver.
- Full Text:
- Date Issued: 2014