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Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum

  • Authors: Afolayan, Omolola Folasade
  • Date: 2013
  • Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
  • Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
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  • Date Issued: 2013

Modelling the performance of a calorifier installed at a university residence intended to be retrofited by an air source heat pump

  • Authors: Mzolo, Noluthando Precious Duduzile
  • Date: 2017
  • Subjects: Caloric engines Heat pumps
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/4660 , vital:28485
  • Description: Sanitary hot water production contributes significantly to the electrical energy consumption in the university campus. An in-depth understanding of the current hot water technology, number of students, capacity of water used per student, time of use of hot water, total volume of hot water consumed and the total energy used on an average weekday in a university residence are very crucial in opting for energy efficient technology like an air source heat pump (ASHP) water heater. The study focused on quantitative and qualitative analysis of data collected for the hot water profiles in one of the university residences (Elitheini 1) from the conduction of experiment and questionnaires. The results revealed that 94% of the 75 students used hot water during the Eskom morning peak and 61% during the Eskom evening peak. In addition, the average daily energy consumption of the 12 kW Calorifier was 139.49 kWh, while the measured volume of hot water usage was 1950 L which is in strong agreement with the total volume (1945 L) of hot water consumption from the questionnaires. The p-value of the average volume of hot water usage measured by experiment and questionnaires was 0.7 and is of no mean significant difference. The monthly energy consumption for the week days was projected to be 2929.31 kWh. By retrofitting of calorifier with an ASHP unit, the energy consumption could reduce to 976.43 kWh based on its consecutive coefficient of performance of 3. Finally, from the energy consumption reduction analysis and the current Eskom tariffs, it can be alluded that the payback period of the proposedASHP unit as a retrofit to the calorifier is going to be less than 2 years and is worthy to invest into such technology under this study due to its favourable payback period and the reliability and lifespan of the ASHP unit.
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  • Date Issued: 2017

The study of hydroxyoximes and hydroxamic acids supported on macroporous resins and their use in the rapid seperation of metals

  • Authors: Hemmes, Marlene
  • Date: 1979
  • Subjects: Chromatographic analysis , Separation (Technology) , Metal ions , Solvent extraction , Extraction (Chemistry) , Oximes , Metals -- Analysis
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4453 , http://hdl.handle.net/10962/d1009512
  • Description: Introduction: The macroporous Amberlite XAD resins were coated with LIX-64N and examined for the rate of uptake of copper . XAD-7 was by far the best support and gave a satisfactory rate of uptake up to loadings of 60% (w/w). The specific surface area of XAD-7 was measured by the adsorption of methylene blue from aqueous solution. The area of the wetted resin was five times less than that of the dry resin. LIX-65N was purified and the anti isomer characterised using spectroscopic techniques . The rate of uptake of copper was not improved by use of purified LIX-65N or by addition of LIX-63. XAD-7 coated with LIX-65N was used in columns. Elution curves for copper showed negligible tailing, and rapid separations of copper from iron (111), nickel, cobalt and magnesium by selective absorption were achieved. Copper was concentrated from very dilute solution at a flow rate of 50 ml min -1 ,and a 99% recovery was obtained. The method was applied to the rapid determination of copper in brass and bronze. A series of long-chain hydroxamic acids were synthesised and tested for suitability as stationary phase on XAD-7. Oleohydroxamic acid and naphthenohydroxamic acid were the most promising. The r ate of uptake of copper was reduced by the use of nonylphenol or amyl alcohol as a diluent. The capacities for copper of the hydroxamic acids were less when supported on XAD-7 than when used as liquid ionexchangers. The distribution coefficients of cobalt, nickel, zinc, lead, vanadium, uranium, iron (111) and copper were measured as a function of pH. XAD-7 coated with oleohydroxamic acid was used in columns for the rapid separation of iron (111) from copper and of copper from nickel, cobalt, lead and zinc. Copper was concentrated from very dilute solution at a flow rate of 45 ml min -1 and a 100,8% recovery was obtained. Copper was successfully separated from nickel by selective elution. The elution curves obtained show negligible tailing. The resin loaded with oleohydroxamic acid lost capacity due to chemical instability. Naphthenohydroxamic acid supported on XAD-7 was not suitable for use in columns, because it was physically unstable.
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  • Date Issued: 1979

Comparative analysis of characteristics of the various sugarcane bagasse types in terms of gasification

  • Authors: Kula, Mpumezo
  • Date: 2016
  • Subjects: Bagasse Sugarcane -- Breeding Coal gasification
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/13392 , vital:39656
  • Description: The insight that the fossil fuel reserves are limited, together with concerns over security of supply (i.e. the oil crises), initiated the first raise of interest in biomass and all other renewable energy forms. However, the concern grew that global warming and the resulting climate change were enhanced by carbon dioxide (CO2) emissions resulting from fossil fuel consumption. Meanwhile, biomass energy is thought to account for 14–15percent of total world energy consumption. Hence, the exploitation and utilization of biomass energy are effective and necessary for relieving the pressures caused by environmental pollution and fossil fuel shortage (Lv et al., 2010). Recently, extensive research aimed at converting biomass to useful energy have been carried out, especially pyrolysis and gasification, which are particularly suitable for the effective and efficient utilization of biomass. Sugarcane bagasse is the main byproduct of sugarcane mill, ready available at the production site so that it may be a suitable raw material for the production of biofuels, chemicals, and electricity. Among the methods that have been previously studied, is this particular study we explore thermochemical process, gasification. The properties of sugarcane bagasse relevant to gasification are briefly reviewed. The compositions of the bagasse as a fuel are variable, especially with respect to inorganic constituents important to the critical problems of sintering, fouling and slagging. Alkali and alkaline earth metals, in combination with other fuel elements such as silica and sulfur, are responsible for many undesirable reactions in a gasifier system. The three sugarcane bagasse types namely washed, unwashed and the depithed sugarcane bagasse were subjected in different types of characterization to review and compare their characteristics for gasification. Ultimate analysis was used to determine the elemental compositions and this was helpful in estimating the energy input of the materials. Elemental compositions were determined with the CHNS analyzer which gave the percent compositions of each fuel in terms of Carbon, Hydrogen, Nitrogen, Sulphur as well as Oxygen (by difference) and the SEM/EDX which gave the elemental composition of the materials as can be viewed from the EDX. The proximate analysis which is the physical characterization of the materials was carried out to preview the behavior of the sugarcane bagasse when subjected into high temperatures as the gasification process uses very high temperatures. These were determined by the TGA and the volatile matter content; fixed carbon content, moisture content and ash content were extrapolated from the resulting thermogram. SEM images for surface structures of the bagasse and FTIR for the structural functionality and bonding structures. The characterization of the bagasse was conducted to compare their gasification properties.
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  • Date Issued: 2016

An investigation into the anxiolytic properties of melatonin in humans

  • Authors: McCallaghan, Johannes Jacobus
  • Date: 1999
  • Subjects: Melatonin , Pineal gland -- Secretions
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:3772 , http://hdl.handle.net/10962/d1003250 , Melatonin , Pineal gland -- Secretions
  • Description: The purpose of this project was to investigate the role of melatonin in the pathophysiology of anxiety in humans. The literature study confirmed the intimate relationship between serotonin and melatonin. Melatonin is not only able to act as an agonist (in physiological concentrations) and an antagonist (at higher concentrations) on serotonin receptors but via control of brain pyridoxal kinase activity might have an effect on GABA, serotonin, dopamine and norepinephrine synthesis. A clinical trial to investigate melatonin's effect on anxiety in humans was conducted as a pilot study. Thirty patients complaining of anxiety participated in a liN of 1" double blind placebo controlled trial. During the experiment each subject was thus exposed to melatonin and a placebo for a week at a time on two occasions. During the first phase of the experiment, (Pair '1) patients showed a statistically significant reduction in their anxiety levels during the first period (P1P1), which was not the case during the second period (P1P2). The improvement however continued during the second phase of the experiment (Pair 2) so that there was also a statistically significant improvement during P 2 P 2 (Period 2 / Pair 2) when placebo was administered. It could not conclusively be shown that melatonin was responsible for the improvement in the patients' anxiety. The explanation for these results suggests thelt the improvement was due to a: 1) placebo effect throughout, 2) psychotherapeutic effect due to contact with a clinician, 3) melatonin induced phase shift in the patient's endogenous melatonin response curve, 4) combination of all 3 options. This pilot study lays the groundwork for a much more exhaustive study in which the melatonin of the patients is determined before melatonin is administered, the role of the clinician is clarified and the most appropriate time for melatonin administration is sought .
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  • Date Issued: 1999

Mineralogy and geochemistry of permian black shales and carbonate concretions in the lower ECCA formations of the Steytlerville- Jansenville area, southern Karoo basin

  • Authors: Maake, Laurentias Tebatso
  • Date: 2019
  • Subjects: Black shales -- South Africa -- Jansenville , Geochemistry Chemistry, Analytic Mineralogy -- Analysis
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10948/40643 , vital:36206
  • Description: The Lower Permian Ecca Group formations of the Karoo Basin of South Africa have recently been identified as a target for shale gas exploration. These units, named the Prince Albert, Whitehill and Collingham formations, comprise organic-rich shales with occasional associated carbonate deposits, and siliciclastic facies. Mineralogical and geochemical investigations were conducted into carbonaceous shales and associated sedimentary rocks in the Jansenville area of the southern Karoo Basin with the ultimate aim to decipher the paleoenvironment and post-depositional conditions of these shales and their associated features such as prominent intra-formational carbonate deposits. Sediments of the main Karoo Basin were deposited from ~350 Ma to ~182 Ma, the end of sedimentation being marked by eruption of basaltic lava. This period, which began with the Dwyka continental glaciation, included tectonism of the Cape Fold Belt, the end-Permian mass extinction at ~250 Ma, and major intrusion of dolerite associated with the lavas of the Karoo Large Igneous Province. Subsequent to this, the basin experienced faulting associated with Gondwana breakup, uplift and intrusion of small volume kimberlite and melilite magmas, and erosion resulting in formation of a major escarpment. Each episode was imprinted upon the Karoo rocks and to a greater or lesser extent erases the signature of older episodes. To decipher the depositional paleoenvironment and post-depositional conditions of the black shales and the dolomite concretions posed a challenge due to deformation, orogeny, metamorphism, and weathering and erosion. Therefore, deep borehole core-logging and sampling was generally preferred over surface fieldwork, and trace elements backed up by scanning electron microscopy-based petrography was the method most relied upon to decipher the redox conditions of the black shales and the intra-formational carbonates. Relevant sections from three SOEKOR boreholes SP1/69, AB1/65 and QU1/65 were logged and carbonate concretions localities studied in four field locations. Samples collected from the core and field localities were prepared for thin section optical, SEM petrographical analysis, mineral identification, modal estimation by XRD, major, and trace element analysis by XRF and Laser Ablation-ICPMS, and acid leaching of a sample subset to determine the degree of pyritization (DOP). Logging of the SOEKOR boreholes indicates that in the western part of the basin all three lower Ecca formations, namely the Prince Albert, Whitehill and Collingham, overlie the tillites of the Dwyka Group, whereas near East London only the Whitehill Formation is present. Dwyka diamictites occur in all the studied boreholes overlying the crystalline basement in AB 1/65 and QU 1/65 boreholes whereas in the SP 1/69 the tillites rest above the quartzite of Witteberg Group of the Cape Supergroup. The shale consists of discontinuous, wavy and straight parallel laminae. Parallel, discontinuous and elongate micro-lenses of very fine-grained quartz are diagnostic and suggest late-stage silicification. Thin laminae of black shale are interlaminated with grey clay. The black shales are composed of quartz and clay minerals (illite and chlorite) as the major crystalline minerals with minor quantities of sulphides and heavy minerals. Organic matter occurs as unstructured, anhedral patches of amorphous material. It contains abundant small (~10nm) and less common larger (~100 nm) pores of subsphaeroidal shape. Carbonates occur as cementation and concretions. Heavy minerals identified by SEM include detrital zircon, thorite, titanite, authigenic fluorite, galena, sphene, and sphalerite and apatite, monazite and epidote-group minerals that appear to be of later stage metamorphic origin, some a product of hydrothermal feldspar alteration. Zircon and monazite show evidence of partial corrosion and/or new overgrowth. Titanite occurs in greater abundance in the SP1/69 section than other boreholes, where it exhibits a porphyroblastic texture suggesting secondary growth. These features all suggest modification of detrital minerals by metamorphic fluids and therefore some possible modification of bulk geochemical composition. Pyrite is abundant, commonly occurring as framboidal and occasional euhedral grains. Pyrite-bearing, calcite veins are common in the Whitehill Formation. Some pyrite is metamorphosed to pyrrhotite in the shales adjacent to dolerite intrusions. The carbonate deposits in the lower Ecca occur mostly as large concretions of 0.5-2.5 m in diameter at intensely faulted areas, and as laterally continuous beds at less intensely faulted areas. They consist of dominant dolomite with calcite (differentiated by thin section staining) and minor associated quartz veins, and appear to have formed in an early diagenetic stage of the black shale. Five different dolomite-rock textures were identified indicating varying crystal growth conditions. Calcite cementation types accompany these dolomite textures. The major types include mosaic, sparry and bladed/prismatic calcite cement. Calcite occurs mainly as cement in pores and grain replacement, as well as crudely radial septerian veins. XRD indicates that the carbonate concretions are made up of more than 90% dolomite, especially within the intensely folded areas. The remaining 10% consist of post-depositional quartz veins and secondary calcite associated with the quartz veins. In one locality (VAAL) the carbonate samples are dominates by bladed/prismatic calcite. The black shales in this study have similar geochemical signature to previously analysed samples from the Karoo Basin and to black shales worldwide. The geochemical signature results from the combined input of detrital sedimentary materials as well as enrichment or depletions acquired from pore water, biological activity and during diagenesis. Positive correlation of K2O, Na2O with Al2O3 suggests control by the detrital contribution. CaO and P2O5, have a negative correlation with Al2O3 suggesting a biogenic source. Elemental ratios suggest that the sediments derive from felsic source rock, most likely Cape Supergroup and underlying crystalline basement. These shales exhibit different degrees of trace-element enrichment relative to global average shale, the approximate order being Pb> V> Zn> Cr> Cu> Co > Ni. Assessment of selected trace elements, V/(V+Ni), V/Cr, Ni/Co and EF Mn ratios describes the paleoenvironment of these sediments to have been partially oxygenated before sulphate reduction interface. The pyrite size distribution, DOP, Fe and S correlation points to an organic matter limited environment, where the main sulphur sink is pyrite rather than organic matter. The DOP and Fe/Al indicated that the sediments became anoxic at the sediment-water interface (early diagenetic stages). V/Cr assessment of the dolomite concretions suggested partially oxygenated environment corresponding to the black shale deposition. The occurrence of concretions at the base of the Whitehill Formation, which has the highest TOC content of 14% compared to other associated formations, is evidence that organic matter preservation was important to their origins. The parameters used here to assess the redox conditions of the black shales and the dolomite concretions in this region of the Karoo Basin suggest a normal marine redox environment, rather than the anoxic Black Sea-type environment. The conditions that affected the lower Ecca formations varied from semi-oxygenated to oxygen depletion environment. The oxygen depletion environment occurred during the deposition of the Whitehill Formation of which organic matter preservation was favoured..
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  • Date Issued: 2019

Problems experienced by project managers on housing projects

  • Authors: Mtshekexe, Ntombesibini Octavia
  • Date: 2014
  • Subjects: Project management , Housing rehabilitation , Public housing -- South Africa , Building -- Superintendence
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:9675 , http://hdl.handle.net/10948/d1020930
  • Description: Housing projects are amongst the South African (SA) Government’s major focus points with reference to budgeting for its financial years. The delivery of these housing projects is taking place at a slower rate than anticipated due to the problems experienced in the processes involved in their management. The project management shortcomings are a major setback with reference to the delivery of houses to the communities. It is, however, stated by the Constitution of the Republic of South Africa (RSA) that every South African citizen has a right to housing and that the Local Government, as the closest entity to communities, must do whatever it possibly can to ensure timeous service delivery to its communities. The objective of this research is to ascertain the nature and extent of project management problems experienced by municipal project managers, the impact thereof and potential solutions to the identified problems. The study is intended to advise on possible ways to address project management problems arising during the implementation of housing projects in the Nelson Mandela Bay Municipality (NMBM) through investigation and analysis of information gathered in both theory and practice. Project management is a process of controlling the achievement of project objectives through the application of knowledge, skills, tools and techniques. For a housing project the main objective is to deliver safe, quality houses on time and within budget. Problems which hinder the delivery of housing projects relate to cost, time and the quality of housing projects delivered. The fact that these key project objectives are not met needs to be addressed urgently in order to improve the housing delivery. The literature reviewed and the feedback from the surveys; indicate that these problems have a vast impact on housing projects. The survey also reveals that it is not only housing Project Managers (PMs) that are unable to perform well, but also other parties involved in the project management of housing projects and which play a great role in project management failures of housing projects. Some of these parties are consultants and contractors. The lack of performance by project managers affects the delivery of houses negatively in the NMBM.
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  • Date Issued: 2014

Uptake of liposomes into bacterial cells

  • Authors: Oidu, Benjamin
  • Date: 2013
  • Subjects: Liposomes , Drug carriers (Pharmacy)
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:10165 , http://hdl.handle.net/10948/d1021010
  • Description: Liposomes are small phospholipid vesicles that have been widely investigated as drug carriers for the delivery of therapeutic agents. A variety of liposome formulations are presently under clinical trial exploration, while others have already been approved for clinical use. The aim of this study was to optimize liposome uptake into bacterial cells. Both gram-positive and gram-negative bacteria were used in the study as well as Candida albicans.Response surface methodology (RSM) using a central composite design (CCD) model was used to optimize liposomal formulations of carboxyfluorescien (CF) for each of the three microbes, and also the three microbes in combination namely; Staphylococcus aureus (Sa), Escherichia coli (Ec) and Candida albicans (Ca). Percentage of CF encapsulated and CF increase in Uptake were investigated with respect to two independent variables that were, cholesterol (CHOL) and stearylamine (SA) content. Design Expert 8 was used for the purpose of finding the combination of independent variables that would yield an optimal formulation for each microbe and the three microbes in combination. The model selected by the software managed to reasonably correlate the predicted models to the experimental data. Encapsulation of carboxyfluorescien (CF) into a liposome formulation enhanced its uptake by Staphylococcus aureus and Escherichia coli as well as Candida albicans. This was evident in the increase in CF uptake when the uptake rate of free CF was compared with that of liposomal CF.
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  • Date Issued: 2013

Polyunsaturated fatty acid metabolism and effects on colon cancer cell biology in vitro.

  • Authors: Bulcao, Candice
  • Date: 2013
  • Subjects: Unsaturated fatty acids , Unsaturated fatty acids in human nutrition , Colon (Anatomy)-- Cancer , Cancer -- Nutritional aspects
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4137 , http://hdl.handle.net/10962/d1016128
  • Description: Colon cancer is a leading cause of cancer related deaths worldwide. Lifestyle factors such as diet and exercise have been implicated as important agents in colon cancer development and progression. Epidemiological, in vivo and in vitro studies have found that n-3 polyunsaturated fatty acids (PUFAs) reduce colon carcinoma. The role of n-6 PUFAs remains a controversial topic, with studies indicating both promoting and preventing capabilities published. In order to better understand the effects of PUFAs on colon carcinoma, it is important to have an understanding of how they will be broken down in the body. During this study, in silico metabolism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) predicted the formation of hydroxy-, di-hydroxy- and epoxy-FAs. A gas chromatography-mass spectrometry method was developed and validated for the detection of these PUFAs and their cytochrome P450 (CYP) metabolites. A human liver microsomal system for the in vitro metabolism of EPA, DHA and AA was optimised in terms of microsomal and PUFA concentration. The system resulted in the metabolism of the positive control, lauric acid, to 12-hydroxy-lauric acid but was unable to metabolise the PUFAs of interest. EPA, DHA and AA reduced cell viability in the colon carcinoma cell lines SW480 and SW620 in the micromolar concentration range (25 – 200 μM). The CYP epoxidation metabolite of EPA, 17, 18-epoxyeicosatetraenoic acid (17, 18-EpETE) resulted in a significant reduction in SW480 cell viability relative to the parent compound at lower concentrations (25 and 50 μM). Annexin V apoptosis analysis revealed that EPA and 17, 18- EpETE did not result in apoptosis in SW480 cells at a concentration of 25 μM and over an incubation period of 24 hours. A significant reduction in reactive oxygen species production was seen in SW480 cells after incubation with 25 μM 17, 18-EpETE for 24 hours. EPA and 17, 18-EpETE were implicated in the reduction of colon cancer metastasis since they were able to reduce SW480 migration and anchorage independent cell growth. These results indicate that the dietary intake of EPA, DHA and AA may be beneficial to one’s health due to the negative effects that these PUFAs had on colon carcinoma. Future studies are needed to confirm these benefits and compare the effects of the PUFAs to their CYP-metabolites.
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  • Date Issued: 2013

Early stage ichthyofauna from shallow water habitats of the Angola-Benguela frontal zone

  • Authors: Farthing, Matthew William
  • Date: 2017
  • Subjects: Fishes -- Larvae -- South Africa -- Atlantic coast , Fishes -- Larvae -- Angola -- Atlantic coast , Striped mullet -- Angola -- Atlantic coast
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10962/50442 , vital:25988
  • Description: Early stage fishes (larvae and early juveniles) were collected from an array of shallow water (±1.5m) habitats on the warm-temperate southern Angolan coastline between June 2014 and June 2015. This study, the first assessment of the early stages of coastal fishes in Angola, provides important new understanding of non-estuarine shallow water nursery habitat use by coastal fishes from the region. In total, 51 species from 18 teleost families were observed. The exposed surf zone was dominated by postflexion larvae, while the moderately exposed beach and sheltered bay were dominated by early juveniles. Both fish density and diversity peaked in the wet season (Feb – Apr 2015) for all habitats, attributed to the influx of summer spawning species. Diplodus sargus dominated catches from the exposed surf zone, and the timing of observations supports the growing consensus that sparid spawning is dictated by temperature, not season. The similitude of the observed species to those observed in warm temperate South Africa highlights the historical connection of the warm-temperate study area with warm-temperate South Africa, prior to the formation of the Benguela Current as a vicariant barrier. The classification of the study assemblage into established estuarine utilization categories showed that the surf zone hosted more exclusively marine species than comparable surf zones in South Africa, attributed to the absence of estuaries in southern Angola. However, the observation of the marine estuarine dependent Mugil cephalus in this study area devoid of estuaries suggests that estuarine dependency may be regionally specific for some taxa. It appears that the warm, sheltered, nutrient rich waters of Tombua Bay provide typically estuarine associated taxa with a suitable alternative, non-estuarine nursery habitat. This study provides evidence that the importance of estuaries as critical nursery habitats has been overemphasized for some warm-temperate coastal fishes. However, the absence of other marine estuarine dependent species from the study area suggests that some warm-temperate taxa do intrinsically rely on estuarine function for survival.
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  • Date Issued: 2017

The growth characteristics of sparid otoliths

  • Authors: Lang, Judy Brenda
  • Date: 1992
  • Subjects: Fishes -- Age determination , Otoliths , Sparidae
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5285 , http://hdl.handle.net/10962/d1005129 , Fishes -- Age determination , Otoliths , Sparidae
  • Description: The periodicity of formation of growth increments in the otoliths of South African sparids was validated by the oxytetracycline labelling technique. Intramuscular injections of oxytetracycline at a dosage of 250mg/kg marked the otoliths of laboratory held juvenile sparids, while a dosage range of 50 - 100mg/kg oxytetracycline, injected intramuscularly, marked adult sparids in the field. Laboratory held, larval sparid otoliths were marked by immersion for 24hrs a solution of 100-150mg/l alizarin complexone. Both daily (microstructural) and annual (macrostructural) growth increments were identified in the otoliths. The micro incremental pattern of deposition in the sparid otoliths conformed to the general pattern of otolith structure. Sparid otoliths consisted of a central opaque nucleus composed of multiple primordia. surrounding this nucleus were daily increments which decreased in width as the distance from the nucleus increased. Both check rings and subdaily increments were visible throughout the otolith. Minor environmental changes did not affect micro incremental deposition. Narrow opaque and wide hyaline annual growth zones were identified in sectioned sparid otoliths. Scanning electron microscope analysis of the annual growth zones revealed that microincrements within the opaque zone were narrowly spaced with prominent discontinuous phases. This resulted in the greater optical density and higher protein content of the zone. The hyaline zone was composed of widely spaced daily increments with prominent incremental phases accounting for the translucent nature of this zone. Opaque zone formation in the otoliths of many South African sparids was found to occur primarily during periods of reproductive activity and was shown to be indicative of slow otolith growth. The hyaline zone was formed after the spawning season, representative of fast otolith growth. The results of this study have resolved much of the controversy surrounding the rate of growth and time of formation of the opaque and hyaline growth zones in South African sparid otoliths.
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  • Date Issued: 1992

The high pressure catalytic hydrogenation of the tannin of black wattle (Acacia mollissima Willd)

  • Authors: Silk, M H
  • Date: 1947
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:21132 , http://hdl.handle.net/10962/6528
  • Description: From Introduction: The wattle industry in South Africa has, from its commencement in the middle of the last century, gradually assumed a position of increasing importance in the economic structure of the country. Apart from the provision of a valuable tanning extract for home use and for export, the tree itself has proved of considerable importance to the Union’s mining industries. In addition there would appear to be possibilities for the utilisation in the near future, of the raw ground bark and the wattle extract for the manufacture of a number of by-products, including certain types of plastics.
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  • Date Issued: 1947

A comparison of web-based technologies to serve images from an Oracle9i database

  • Authors: Swales, Dylan
  • Date: 2004 , 2013-06-18
  • Subjects: Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4583 , http://hdl.handle.net/10962/d1004380 , Active server pages , Microsoft .NET , JavaServer pages , Oracle (Computer file) , Internet searching , Web site development--Computer programs , World Wide Web , Online information services
  • Description: The nature of Internet and Intranet Web applications has changed from a static content-distribution medium into an interactive, dynamic medium, often used to serve multimedia from back-end object-relational databases to Web-enabled clients. Consequently, developers need to make an informed technological choice for developing software that supports a Web-based application for distributing multimedia over networks. This decision is based on several factors. Among the factors are ease of programming, richness of features, scalability, and performance. The research focuses on these key factors when distributing images from an Oracle9i database using Java Servlets, JSP, ASP, and ASP.NET as the server-side development technologies. Prototype applications are developed and tested within each technology: one for single image serving and the other for multiple image serving. A matrix of recommendations is provided to distinguish which technology, or combination of technologies, provides the best performance and development platform for image serving within the studied envirorunent. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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  • Date Issued: 2004

The acute impact of extended aerobic exercise on cognitive performance

  • Authors: Tichiwanhuyi, Tendayi Stephen
  • Date: 2015
  • Subjects: Aerobic exercises , Cognition -- Effect of exercise on , Exercise -- Physiological aspects , Exercise -- Psychological aspects , Visual perception , Short-term memory , Motor ability , Human information processing
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5173 , http://hdl.handle.net/10962/d1018182
  • Description: Previous research has established a relationship between exercise and cognition, with more emphasis on this ascertained link, being made on the effects of long term and endurance exercise on cognition. However, enhanced worker effectiveness relies on a strong acute collaboration of physical and cognitive performance during task execution. As a result, the purpose of this study was to examine the acute effects of extended aerobic exercise on visual perception, working memory and motor responses, and to achieve this 24 participants (12 males and 12 females) aged between 18 and 24 participated in a 2 bout cycling exercise. The experimental condition had cycling resistance set at 60 percent of each individual’s maximum aerobic output and the control condition had zero cycling resistance, where three cognitive tasks were performed at 10 minute intervals during the cycling exercise. The results showed that exercise did not affect any significant changes on the cognitive performance measures over the entire cycling duration, as well as during the exercise phase (cycling with resistance). However, visual perception improved significantly (p<0.05) immediately after exercise. This led to the conclusion that moderate to high intensity exercise when performed for an extended duration, has selective effects on certain cognitive performance measures, with the time at which the performance is measured during the exercise being a relevant factor to be considered for maximum activation effects of the exercise.
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  • Date Issued: 2015

The development of sustainability criteria to facilitate the selection of sanitation technologies within the Buffalo City Municipality (Eastern Cape Province, South Africa)

  • Authors: Hoossein, Shafick
  • Date: 2009
  • Subjects: Sanitation -- South Africa -- Buffalo City Sustainable development -- South Africa -- Buffalo City Water -- Purification -- South Africa -- Buffalo City Sewage disposal -- South Africa -- Buffalo City Water quality -- South Africa -- Buffalo City Environmental health -- South Africa -- Buffalo City Environmental management -- South Africa -- Buffalo City
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:32275 , http://hdl.handle.net/10962/d1004595
  • Description: Two and a half billion people mostly in sub-Saharan Africa and southern Asia remain without improved sanitation facilities despite the Millennium Development Goal 7's target to halve this number by 2015. While it might be tempting for developing countries such as South Africa to implement the cheapest and most rapidly constructed sanitation services simply to meet the desired target, this could have significant negative implications on human health and the environment over the long-term. As a result, there is a need to ensure that the most appropriate sustainable sanitation technologies are selected during the planning stage. The purpose of this research was therefore to document the development and pilot application of a flexible context-specific decision-support tool for sustainable sanitation technology selection within Buffalo City Municipality (BCM) in the Eastern Cape Province of South Africa. The first step in the development process was to ascertain the current status of sanitation within the municipality, with a specific focus on the main challenges related to the provision, maintenance and performance of these technologies. Thereafter, a participatory approach was employed involving BCM stakeholders to develop a series of sustainability criteria and indicators that took into consideration economic, social, environmental and technical concerns as well as legal requirements. The development process resulted in a list of 38 BCM sustainable sanitation selection criteria that were applied in a pilot study involving rural, urban and peri-urban communities within BCM. Certain criteria related to topographical features and the availability of piped water and land were considered useful for 'coarse screening' while others were applied during 'fine screening'. In order to enhance the context specificity of criteria, each was weighted, through consultation with key BCM stakeholders. This research confirmed that the sanitation situation in BCM was poor and preliminary evidence indicated that sanitation systems were negatively impacting on the quality of water resources within BCM justifying the need for a sustainable sanitation decision support tool. Based on the pilot application of the BCM Sustainability Selection Criteria List, the urine diversion technology was considered the most sustainable technology option in each study site. There were however, certain social criteria that received relatively low sustainability scores and these would need to be addressed prior to the approval of this technology for implementation. The approach adopted in this thesis was considered highly context-specific yet flexible and appropriate for adoption not only by BCM but other municipalities on a range of spatial scales.
  • Full Text:
  • Date Issued: 2009

Identification of differentially expressed proteins involved in wheat (Triticum aestivum) resistance against Russian Wheat Aphid (Diuraphis noxia) SA2 using SWATH-MS analysis

  • Authors: Ntlokwana, Sitha Emmanuel
  • Date: 2020
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/18872 , vital:42888
  • Description: Wheat (Triticum aestivum) is the second most-consumed cereal crop in the world, second only to maize. In South Africa it is mainly produced in three provinces; the Free State, Western Cape, and the Northen Cape. The average total land area used for wheat cultivation in these three Provinces, is 533 000 hectares, with a resultant annual production of between 1.3 to 2 million tons. The total wheat requirement in South Africa is currently 2.7 million tons according to the production of wheat guidelines published by the Department of Agriculture, Forestry and Fisheries, which is higher than the total annual production. One of the primary reasons that contribute to the lower production levels is insect and pest infestation, in particular, the Russian wheat aphid (Diurophis noxis), which can result in crop loss of up to sixty percent. Russian wheat aphids (RWA) are invasive insects that feed on the phloem of the plant through their specialised thin stylet-like mouthparts and release toxic agents contained in their saliva during feeding, which disrupt cellular functions inside the host plant. This damage leads to symptoms like; chlorosis, necrosis, wilting, stunting and curling of leaves, also known as leaf rolling. This study aimed to identify differentially expressed proteins in resistant and susceptible wheat (Triticum aestivum) cultivars during Russian wheat aphid Biotype South African 2 (RWA-SA2) infestation, in order to identify proteins involved in the wheat resistant mechanism against RWA-SA2. Two wheat cultivars SST398 (resistant to RWA-SA2) and SST356 (susceptible to RWA-SA2) were used, and a total number of 126 plants were planted and divided according to four different harvest times (Control day 0; day 5; day 7 and day 12). The wheat plants were infested with approximately 10 aphids per plant at the three-leaf stage, and placed within an insect cage inside a growth chamber (Conviron, Winnipeg, Canada) set at 24ºC, light intensity of 352 µmol.m-2.sec-1, ambient CO2 levels (410 ppm) and humidity of 60% with a 16h/8h day/night cycle. Wheat leaves were harvested at various time frames, and total protein extraction was performed. The protein samples were reduced with 10 mM dithiothreitol (DTT) and alkylated with 30 mM iodoacetamide (IAA). Sample clean-up and on-bead trypsin digest were performed on megReSyn Hilic columns, over four hours. The resultant peptides were vacuum dried and resuspended in 2% acetonitrile (ACN) before submitting them to LC-MS/MS (SWATH) for analysis. A total number of 611 proteins were differentially expressed, of which 19 were identified to be involved in the resistance response of wheat, and an additional 20 involved in biotic stress responses. This study showed that reactive oxidative species (ROS) such as hydrogen peroxide and hydroxyl radicals (OH-) play a significant role in the early stages of wheat resistance against RWA-SA2 infestation. They are involved in cell wall strengthening, activation of defence genes, involvement of phytohormone signals such as salicylic and jasmonic acid, which also mediates the systemic defences such as, systemic acquired resistance (SAR), leading to the expression of pathogen-related proteins such as (thaumatin-like proteins, oxalate oxidase, defensin, chitinase, and thionins). Although up-regulation of photosynthesis proteins such as (chlorophyll a/b binding protein, photosystem I and II, rubisco and divinyl chlorophyllide a, and 8 vinyl-reductase) were seen on both infested cultivars (susceptible and resistance), higher numbers of these classes of proteins were identified in the resistance cultivar, suggesting that they also play a critical role in resistance. In conclusion, the SWATH analysis used in this study was able to identify numerous proteins involved in the wheat resistance mechanism against RWA-SA2, most of which have not yet been reported to be involved in either biotic stress or RWA-SA2 resistance responses. Future studies are required to biochemically confirm the involvement of these enzymes and proteins the specific metabolic pathways where they are found, in the wheat resistance mechanism against Russian wheat aphid Biotype 2
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  • Date Issued: 2020

Assessing linkages between local ecological knowledge, HIV/AIDS and the commercialisation of natural resources across Southern Africa

  • Authors: Weyer, Dylan James
  • Date: 2012
  • Subjects: AIDS (Disease) -- Africa, Southern , HIV infections -- Africa, Southern , Natural resources -- Management -- Africa, Southern , Traditional ecological knowledge -- Africa, Southern , Households -- Economic aspects -- Africa, Southern , Poverty -- Environmental aspects -- Africa, Southern , Ecology -- Economic aspects -- Africa, Southern
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:4769 , http://hdl.handle.net/10962/d1007180 , AIDS (Disease) -- Africa, Southern , HIV infections -- Africa, Southern , Natural resources -- Management -- Africa, Southern , Traditional ecological knowledge -- Africa, Southern , Households -- Economic aspects -- Africa, Southern , Poverty -- Environmental aspects -- Africa, Southern , Ecology -- Economic aspects -- Africa, Southern
  • Description: That natural resources (NRs) are important to those experiencing adversity, and, especially, vulnerability associated with HIV/AIDS, is well documented, particularly with respect to food and energy security. What is unclear is where HIV/AIDS ranks in terms of its significance in comparison to other household shocks, the role local ecological knowledge may (LEK) play in households' response to shock, a propos the types of coping strategies that are employed. Consequently, this research aims to bridge the knowledge gap between HIV/AIDS and the degree to which it is contributing to the expansion of NR commercialisation and to explore the unknowns surrounding the influence of LEK on people's choice of coping strategy. A two phase study was designed to provide quantitative rigour with qualitative depth. Phase one was an extensive, rapid survey of NR traders within urban and rural settings in five southern Africa countries. The principle objective was to profile the trade, the livelihoods of those involved and their reasons for entering the trade, to ultimately establish to what degree HIV/AIDS may have been a catalyst for this. Almost one third of the sample entered the trade in response to illness and/or death in their households, with 80% of deaths being of breadwinners. The findings illustrated considerable dependence on the sale of NRs; for almost 60% of the sample it was their household's only source of income. There was evidently increased blurring of the lines between rural and urban NR use with a greater diversity of products being traded in urban areas. Phase two involved in-depth interviews and work with a smaller sample at two sites selected based on the findings from the first phase. It incorporated three groups of households; non-trading, inexperienced trading and experienced trading households. Key areas of focus were household shocks, coping strategies employed in response to these and the role LEK may be playing in the choice of coping strategies. Within a two year period, 95% of households registered at least one shock, of which 80% recorded AIDS-related proxy shocks. Non-trading households were significantly worse-off in this regard, while in the case of non-AIDS proxy shocks, there was no such difference between groups. The most frequently employed coping strategy was the consumption and sale of NRs and was of particular importance when households were faced with AIDS proxy shocks. Trading households emerged as having superior levels of LEK in comparison to non-trading households, even for non-traded NRs, suggesting that prior LEK of NRs opened up opportunities to trade in NR as a coping strategy. Further inspection of the latter group however revealed that the portion of non-trading households who traded on a very ad hoc basis actually had comparable levels of LEK to the trading households. Despite the ad hoc trading households' vulnerable state and their disproportionately high level of AIDS proxy measures, they had at their disposal, sufficient LEK to unlock certain key coping strategies, namely the NR trade. In this sense there are apparent linkages between LEK, HIV/AIDS and the expansion of the commercialisation of NRs.
  • Full Text:
  • Date Issued: 2012

The specification and design of a prototype 2-D MPEG-4 authoring tool

  • Authors: Viljoen, Deon Walter
  • Date: 2003
  • Subjects: MPEG (Video coding standard) , Digital video -- Standards , Multimedia systems
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:11092 , http://hdl.handle.net/10948/d1015741 , MPEG (Video coding standard) , Digital video -- Standards , Multimedia systems
  • Description: The purpose of this project was the specification, design and implementation of a prototype 2-D MPEG-4 authoring tool. A literature study was conducted of the MPEG-4 standard and multimedia authoring tools to determine the specification and design of a prototype 2- D MPEG-4 authoring tool. The specification and design was used as a basis for the implementation of a prototype 2-D MPEG-4 authoring tool that complies with the Complete 2-D Scene Graph Profile. The need for research into MPEG-4 authoring tools arose from the reported lack of knowledge of the MPEG-4 standard and the limited implementations of MPEG-4 authoring tools available to content authors. In order for MPEG-4 to reach its full potential, it will require authoring tools and content players that satisfy the needs of its users. The theoretical component of this dissertation included a literature study of the MPEG-4 standard and an investigation of relevant multimedia authoring systems. MPEG-4 was introduced as a standard that allows for the creation and streaming of interactive multimedia content at variable bit rates over high and low bandwidth connections. The requirements for the prototype 2-D MPEG-4 authoring system were documented and a prototype system satisfying the requirements was designed, implemented and evaluated. The evaluation of the prototype system showed that the system successfully satisfied all its requirements and that it provides the user with an easy to use and intuitive authoring tool. MPEG-4 has the potential to satisfy the increasing demand for innovative multimedia content on low bandwidth networks, including the Internet and mobile networks, as well as the need expressed by users to interact with multimedia content. This dissertation makes an important contribution to the understanding of the MPEG-4 standard, its functionality and the design of a 2-D MPEG-4 Authoring tool. Keywords: MPEG-4; MPEG-4 authoring; Binary Format for Scenes.
  • Full Text:
  • Date Issued: 2003

Application of hidden Markov models and their extensions to animal movement data

  • Authors: Van Niekerk, Bracken
  • Date: 2018
  • Subjects: Markov processes Animal locomotion Time-series analysis
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10948/23835 , vital:30624
  • Description: Hidden Markov Models (HMMs) have become increasingly popular in animal movement studies as they provide a flexible modelling approach and take the correlation between successive observations into account. They can segment the movement paths into latent states, which can be considered as rough proxies for the behaviours of the animals. This study comprises of two sections, both involving the application of HMMs to large terrestrial mammal movement data. Usually step lengths representing the displacement distances between successive observations, turning angles measuring the tortuosity, or a bivariate input of both variables are used as inputs in the models. It has been found in the literature that the turning angle is either included in the modelling process or it is excluded without much justification for doing so. The first part of this study investigates the nfluence of the turning angle on the model output and resultant interpretations of the HMMs when modelling the trajectories of large terrestrial mammals in southern Africa. Results revealed at different time scales, and for both predator and herbivore species in this study, that the turning angle does not influence the state allocation of the HMMs, which is the main output in terms of interpreting the behaviours of the animals. It is thought in most cases that the inclusion of the turning angle overcomplicates the models unnecessarily without contributing any additional information in terms of the behavioural interpretations or improving the overall fit of the models. This was found for the variety of movements of the species under observation in this study. The second part of this study attempts to validate the state allocation of the HMMs fitted to eland trajectories in the Greater Addo Elephant National Park in the Eastern Cape, with the use of camera trap data. This presented a unique opportunity as this type of data is mainly used for abundance or capture-recapture studies, and the HMMs are rarely validated as the true behaviours of the animals are seldom known. Results revealed that the same diel patterns were detected by the HMMs that were shown by the classified camera trap data. Direct comparisons of the observations where the dates and times matched for the telemetry and camera trap data could be done in several rare instances, which revealed many similarities. Although it was not an ideal comparison, the camera trap data provided a rough validation of the state allocation of the HMMs used in the study.
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  • Date Issued: 2018

Effects of production systems and canola meal supplementation on carcass and meat quality characteristics of spent laying hens

  • Authors: Semwogerere, Farouk
  • Date: 2018
  • Subjects: Broilers (Chickens) -- Feeding and feeds Chickens -- Feeding and feeds Chicken industry -- South Africa
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10353/9242 , vital:34309
  • Description: This study evaluated the carcass and meat quality traits of spent laying hens as influenced by production systems (conventional cages and free range) and the inclusion/exclusion of canola meal in their diets. A total of 30 free range and 60 battery cage reared Lohmann Brown-Elite spent laying hens were obtained from a commercial egg producer. The 30 free range hens (53 weeks of age) and 30 of the caged hens (40 weeks of age) were fed a conventional diet, while the remaining 30 caged hens (48 weeks of age) were fed a diet supplemented with canola meal (20 percent). Carcass, portion and organ weights were determined. Physical attributes and proximate composition were analyzed for, with additional fatty acids and sensory profiles being determined for the effects of canola meal inclusion in the diet. Caged hens had heavier (P ≤ 0.05) warm and cold carcasses, thigh, wing and feet compared to free range hens. The percentages of the breast (26.1 ± 0.51 vs. 28.3 ± 0.28), drum, breast bones, breast thaw and cooking losses and thigh cooking loss were lower (P ≤ 0.05) for caged hens than for free range hens. Free range hens had heavier (P ≤ 0.05) gizzards (33.9 ± 1.04 vs. 30.5 ± 0.73) and bones and a lower (P ≤ 0.05) breast meat percentage (47.3 ± 0.94 vs. 51.7 ± 1.35). Meat redness (a*) (0.54 ± 0.222 vs. 1.40 ± 0.135) and hue angle value, skin redness (a*), breast and thigh, Warner-Bratzler shear force (WBSF) values (breast: 12.37 ± 0.411 vs. 17.10 ± 0.751, thigh: 29.68 ± 0.306 vs. 39.75 ± 0.826), breast moisture and thigh ash content were lower (P ≤ 0.05) for caged hens than free range hens. Caged hens had higher (P ≤ 0.05) thigh thaw loss and breast ash content than free range hens. Canola-fed hens had higher (P ≤ 0.05) drum percentages, breast bone weights and percentages, with lower (P ≤ 0.05) thigh and breast meat percentages. Canola-fed hens had lower (P ≤ 0.05) thaw losses, skin yellowness (b*) and Chroma values, breast fat content with higher cooking losses, skin redness (a*) and hue angle value, as well as breast WBSF (12.37 ± 0.411 vs. 15.43 ± 0.600). Palmitic acid, stearic acid, heneicosanoic acid acid, palmitoleic acid, saturated fatty acids (SFA) (34.0 ± 0.56 vs. 38.7 ± 0.71), n-6:n-3 polyunsaturated fatty acids (PUFA) ratio (5.5 ± 0.13 vs. 7.2 ± 0.28), atherogenic index (IA), thrombogenic index (IT), delta-5 desaturase (D5D, elongase index and thiosterase index were lower (P ≤ 0.05) for canola-fed hen breast meat. Breast meat from conventionally fed hens had lower (P ≤ 0.05) myristic acid, lignoceric acid, nervonic acid, alpha-linolenic acid (ALA), docosahexaenoic acid (DHA), PUFA:SFA ratio (0.7 ± 0.05 vs. 0.9 ± 0.02), n-3 PUFA (3.4 ± 0.31 vs. 5.1 ± 0.17), hypocholesterolemic:Hypercholesterolaemic (h/H), stearoyl-CoA desaturase 16 (SCD16) and stearoyl-CoA desaturase 18 (SCD18). The breast meat from conventionally fed hens had lower (P ≤ 0.05) metallic flavor than that from canola-fed hens. Strong positive correlations were observed for overall aroma with chicken (r = 0.965, P < 0.001) and brothy aroma (r = 0.827, P < 0.001); overall aroma with overall flavor (r = 0.680, P < 0.001), chicken flavor (r = 0.668, P < 0.001) and brothy flavor (r = 0.548, P = 0.006); initial juiciness with sustained juiciness (r = 0.771, P < 0.001) and tenderness (r = 0.537, P = 0.007); sustained juiciness with tenderness (r = 0.790, P < 0.001) and chewiness with residue (r = 0.783, P < 0.001). Whilst strong negative correlations were observed for: sustained juiciness with chewiness (r = -0.655, P = 0.001) and residue (r = 0.783, P < 0.001) and for tenderness with chewiness (r = 0.845, P < 0.001) and residue (r = -0.855, P < 0.001). Results of this study highlight that a free range production system when compared to a conventional cage system increased undesirable carcass and physical meat traits of spent laying hens. On the other hand, canola meal inclusion incorporates beneficial health aspects without affecting the sensory profile of meat derived from spent laying hens, both groups of hens being reared in battery cage system. Spent laying hen breasts can be consumed as a functional food (especially canolafed) since the fat content and composition was observed to be close to that which is recommended for a healthy diet.
  • Full Text:
  • Date Issued: 2018
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