Budget Deficits, the Savings Gap and Current Account Deficits in the Southern African Development Community
- Authors: Kopeledi, Alyssa
- Date: 2020
- Subjects: Budget deficits -- South Africa Accounts current
- Language: English
- Type: Thesis , Masters , MCom (Economics)
- Identifier: http://hdl.handle.net/10353/12392 , vital:39259
- Description: This study examines the validity of the triple deficit hypothesis for a selected number of SADC countries, utilising secondary annual data for the period 1996-2018. Based on previous studies and theory, current account deficit was chosen as the dependent variable and budget deficit and the savings gap as the independent variables. The study makes use of both first generation and second-generation unit root tests to examine the time series properties of the data. The empirical results show that the data is integrated of different orders. Kao, Pedroni and Westerlund cointegration tests were carried out to examine the long-term relationship between the variables of interest. The empirical results revealed that there is a long-term relationship between current account deficit, budget deficit s and the savings gap. The long-run model under the Autoregressive Distributed Lag test revealed that, a positive and significant relationship exists between budget deficit and current account deficit at a significance level of 10% and a negative and insignificant relationship exists between savings gap and current account deficit in the long run. This suggests that an improvement in the current account balance in SADC countries requires fiscal stringency
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- Date Issued: 2020
The predictive ability of the yield spread in timing the stock exchange: a South African case
- Authors: Cook, Jenna
- Date: 2020
- Subjects: Stocks -- Mathematical models , Probits , Johannesburg Stock Exchange
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10962/147025 , vital:38586
- Description: The use of the yield curve in forecasting economic recessions is well established in the literature. A new avenue of use for the yield curve has emerged in the form of using it to forecast bull and bear stock markets. This has the potential to change how investors manage portfolios. A dynamic market-timing strategy would allow investors to shift out of or in to stock markets based on the probability of bear stock market in the future. The relationship between the yield curve and the stock market is tested using an adapted probit model. This has proven positive with encouraging results for the US, India and Spain. This is tested for South Africa using the adapted probit model and the SA yield spread. Bear stock markets are identified on the JSE and forms part of the probit modelling process. Bear markets are identified using a six- and four-month criteria. As South Africa is a small, open and developing economy, the probit is also modelled using the US yield spread. The three probit models do not appear to track bear markets well. This is substantiated through the Henriksson-Merton parametric model test which tests for market timing ability. The results for the SA yield spread using both bear market criteria do not show market timing ability, however, the SA and US yield spread model does show potential market timing ability.
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- Date Issued: 2020
Identification of differentially expressed proteins involved in wheat (Triticum aestivum) resistance against Russian Wheat Aphid (Diuraphis noxia) SA2 using SWATH-MS analysis
- Authors: Ntlokwana, Sitha Emmanuel
- Date: 2020
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/18872 , vital:42888
- Description: Wheat (Triticum aestivum) is the second most-consumed cereal crop in the world, second only to maize. In South Africa it is mainly produced in three provinces; the Free State, Western Cape, and the Northen Cape. The average total land area used for wheat cultivation in these three Provinces, is 533 000 hectares, with a resultant annual production of between 1.3 to 2 million tons. The total wheat requirement in South Africa is currently 2.7 million tons according to the production of wheat guidelines published by the Department of Agriculture, Forestry and Fisheries, which is higher than the total annual production. One of the primary reasons that contribute to the lower production levels is insect and pest infestation, in particular, the Russian wheat aphid (Diurophis noxis), which can result in crop loss of up to sixty percent. Russian wheat aphids (RWA) are invasive insects that feed on the phloem of the plant through their specialised thin stylet-like mouthparts and release toxic agents contained in their saliva during feeding, which disrupt cellular functions inside the host plant. This damage leads to symptoms like; chlorosis, necrosis, wilting, stunting and curling of leaves, also known as leaf rolling. This study aimed to identify differentially expressed proteins in resistant and susceptible wheat (Triticum aestivum) cultivars during Russian wheat aphid Biotype South African 2 (RWA-SA2) infestation, in order to identify proteins involved in the wheat resistant mechanism against RWA-SA2. Two wheat cultivars SST398 (resistant to RWA-SA2) and SST356 (susceptible to RWA-SA2) were used, and a total number of 126 plants were planted and divided according to four different harvest times (Control day 0; day 5; day 7 and day 12). The wheat plants were infested with approximately 10 aphids per plant at the three-leaf stage, and placed within an insect cage inside a growth chamber (Conviron, Winnipeg, Canada) set at 24ºC, light intensity of 352 µmol.m-2.sec-1, ambient CO2 levels (410 ppm) and humidity of 60% with a 16h/8h day/night cycle. Wheat leaves were harvested at various time frames, and total protein extraction was performed. The protein samples were reduced with 10 mM dithiothreitol (DTT) and alkylated with 30 mM iodoacetamide (IAA). Sample clean-up and on-bead trypsin digest were performed on megReSyn Hilic columns, over four hours. The resultant peptides were vacuum dried and resuspended in 2% acetonitrile (ACN) before submitting them to LC-MS/MS (SWATH) for analysis. A total number of 611 proteins were differentially expressed, of which 19 were identified to be involved in the resistance response of wheat, and an additional 20 involved in biotic stress responses. This study showed that reactive oxidative species (ROS) such as hydrogen peroxide and hydroxyl radicals (OH-) play a significant role in the early stages of wheat resistance against RWA-SA2 infestation. They are involved in cell wall strengthening, activation of defence genes, involvement of phytohormone signals such as salicylic and jasmonic acid, which also mediates the systemic defences such as, systemic acquired resistance (SAR), leading to the expression of pathogen-related proteins such as (thaumatin-like proteins, oxalate oxidase, defensin, chitinase, and thionins). Although up-regulation of photosynthesis proteins such as (chlorophyll a/b binding protein, photosystem I and II, rubisco and divinyl chlorophyllide a, and 8 vinyl-reductase) were seen on both infested cultivars (susceptible and resistance), higher numbers of these classes of proteins were identified in the resistance cultivar, suggesting that they also play a critical role in resistance. In conclusion, the SWATH analysis used in this study was able to identify numerous proteins involved in the wheat resistance mechanism against RWA-SA2, most of which have not yet been reported to be involved in either biotic stress or RWA-SA2 resistance responses. Future studies are required to biochemically confirm the involvement of these enzymes and proteins the specific metabolic pathways where they are found, in the wheat resistance mechanism against Russian wheat aphid Biotype 2
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- Date Issued: 2020
Between past and future: memory and mourning in the stories of Okwiri Oduor and Ndinda Kioko
- Authors: Awuor, Nicholas Amol
- Date: 2020
- Subjects: Oduor, Okwiri -- Criticism and interpretation , Oduor, Okwiri -- The plea bargain , Oduor, Okwiri -- My father's head , Oduor, Okwiri -- Rag doll , Kioko, Ndinda -- Criticism and interpretation , Kioko, Ndinda -- Sometime Before Maulidi , Kioko, Ndinda -- Some Freedom Dreams , Authors, Kenyan -- Criticism and interpretation , Kenyan fiction (English) -- History and criticism , Kenyan literature (English) -- History and criticism
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/163168 , vital:41015
- Description: This study investigates the literary activities of two emerging female Kenyan writers, Claudette Okwiri Oduor and Jacqueline Ndinda Kioko, both of whom are award-winning authors. Oduor won the 2014 Caine Prize for African Writing while Kioko bagged the Wasafiri New Writing Fiction Award 2017. It examines specifically how the writers deal with memory and mourning in negotiating between the past and future. I explore how their fictional and non-fictional narratives assist individuals and groups to confront loss, reconstruct new identities, and renegotiate belonging amidst personal and social upheaval. The fictional narratives at the centre of this research are Oduor’s “The Plea Bargain” (2011), “My Father’s Head” (2013) and “Rag Doll” (2014), and Kioko’s “Sometime Before Maulidi” (2014) and “Some Freedom Dreams” (2017). The study explores the themes of mental illness, existential crisis, and fragmentation, and considers bereavement, queer relationships, cultural freedom, and social recognition. The research further considers the active participation of these two writers in Kenya’s contemporary literary-cultural conversations, which span different genres and various media platforms, including blogs, YouTube clips, online magazines, and social media networks in dialogue with other writers. I trace the significance of the literary-cultural link these authors have with their local, continental, and global counterparts in countries like Uganda, Nigeria, and South Africa. The link finds expression through their (in)direct association with some of the new online publishing outlets in Kenya like Jalada Africa, Enkare Review, and Kikwetu. More importantly, their shared participation in and association with such international awards and scholarships as the Commonwealth Short Story Prize, Kwani Trust Manuscript Project, and Miles Morland Foundation is integral in apprehending contemporary literary exchanges and multidirectional flows of publishing in Africa and beyond. I equally illustrate how mentorship of younger writers through local writers’ organisations and collectives like AMKA and Writivism help in the formation of an alternative canon other than the mainstream. The study affirms that the authors seem to transcend the boundaries of production and circulation by fluidly moving between electronic and non-electronic platforms, thus mimicking the memory production of remembering, repeating, and working through.
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- Date Issued: 2020
A strategy to motivate continued instructor usage of learning management systems (LMSS) in higher learning institutions of Zimbabwe
- Authors: Siwela, Ndukuyenkosi
- Date: 2020
- Subjects: Web-based instruction , Computer systems Organizational learning -- Zimbabwe Internet in education
- Language: English
- Type: Thesis , Masters , MPhil
- Identifier: http://hdl.handle.net/10948/47259 , vital:39837
- Description: The purpose of this study was to develop a strategy to motivate continued usage of Learning Management Systems in higher learning institutions of Zimbabwe. A related goal was to identify unique challenges experienced by instructors in their use of LMSs. The use of LMSs is now global and has been fairly successful in developed countries even though past research shows that instructors tend to discontinue usage over a period of time. Whereas most LMSs research is carried in the context of the developed world, the candidate demonstrates that Vantankesh's IS Success Model and Davis' Technology Acceptance Model can be successfully replicated into the developing world on condition that local environment is taken into context. The results showed that LMSs in Zimbabwe higher learning institutions hold a promise of success even though challenges exist. The findings have wider implications on the need to invest in neccessary infrustructure and future predictions on learner interests. The study demonstrates that shared success can be achieved if the local conditions are taken into context when developing a strategy to motivate instructor continued LMS usage. The study holds the practical implication that institutions can motivate instructors to continue with usage of LMSs to deliver quality output in their daily duties.
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- Date Issued: 2020
Technology in conservation: towards a system for in-field drone detection of invasive vegetation
- Authors: James, Katherine Margaret Frances
- Date: 2020
- Subjects: Drone aircraft in remote sensing , Neural networks (Computer science) , Drone aircraft in remote sensing -- Case studies , Machine learning , Computer vision , Environmental monitoring -- Remote sensing , Invasive plants -- Monitoring
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143408 , vital:38244
- Description: Remote sensing can assist in monitoring the spread of invasive vegetation. The adoption of camera-carrying unmanned aerial vehicles, commonly referred to as drones, as remote sensing tools has yielded images of higher spatial resolution than traditional techniques. Drones also have the potential to interact with the environment through the delivery of bio-control or herbicide, as seen with their adoption in precision agriculture. Unlike in agricultural applications, however, invasive plants do not have a predictable position relative to each other within the environment. To facilitate the adoption of drones as an environmental monitoring and management tool, drones need to be able to intelligently distinguish between invasive and non-invasive vegetation on the fly. In this thesis, we present the augmentation of a commercially available drone with a deep machine learning model to investigate the viability of differentiating between an invasive shrub and other vegetation. As a case study, this was applied to the shrub genus Hakea, originating in Australia and invasive in several countries including South Africa. However, for this research, the methodology is important, rather than the chosen target plant. A dataset was collected using the available drone and manually annotated to facilitate the supervised training of the model. Two approaches were explored, namely, classification and semantic segmentation. For each of these, several models were trained and evaluated to find the optimal one. The chosen model was then interfaced with the drone via an Android application on a mobile device and its performance was preliminarily evaluated in the field. Based on these findings, refinements were made and thereafter a thorough field evaluation was performed to determine the best conditions for model operation. Results from the classification task show that deep learning models are capable of distinguishing between target and other shrubs in ideal candidate windows. However, classification in this manner is restricted by the proposal of such candidate windows. End-to-end image segmentation using deep learning overcomes this problem, classifying the image in a pixel-wise manner. Furthermore, the use of appropriate loss functions was found to improve model performance. Field tests show that illumination and shadow pose challenges to the model, but that good recall can be achieved when the conditions are ideal. False positive detection remains an issue that could be improved. This approach shows the potential for drones as an environmental monitoring and management tool when coupled with deep machine learning techniques and outlines potential problems that may be encountered.
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- Date Issued: 2020
Analysis of the existence of the Phillips curve, Okun’s law and Taylor rule in the Zambian economy
- Authors: Chella, Siame Nampasa
- Date: 2020
- Subjects: Unemployment -- Effect of inflation -- Mathematical models --Zambia
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10948/49783 , vital:41800
- Description: The objective for this study was to explore the relationship between inflation and growth with an understanding of the effects of unemployment on growth. The primary objective of this paper was to investigate the existence of Phillips Curve, Okun’s Law, and Taylor Rule in Zambia on the basis that Zambia is a youthful economy, with a growing population, which is expected to temper with unemployment rates, as an increase in the supply of labour might be too high for the demand for jobs available. To be specific, the study aims at determining the relationship between inflation and growth and seeks to understand what policy measures have been undertaken to curb inflation and reduce unemployment in Zambia. To achieve this, a quantitative research was undertaken using the ARDL cointegration model in conjunction with sensitivity tests to cater for structural changes in the economy with 2007 standing as the break point, as well as paying attention to the fact that different policy regimes employed during the course of the data covered, that being, 1996 to 2017, would affect that rates of unemployment, inflation and growth of the country, across period under review. The study revealed that Zambia faces a Hybrid New Keynesian Phillips Curve during the period under study which is dominated by a mixture of both back-ward and forward-looking aspects of inflation. This specifically, paints the effects of inflation persistence as well future expectations of inflation on growth. Further, a cointegration relationship was established between unemployment and output, while Taylor principles were found not to apply to the Zambian economy, which have proved in other economies to be foundation blocks for good economic growth and as such significant to both fiscal and monetary policy authorities of the economy. The researcher therefore recommends that Zambian policy makers, that is, both the fiscal and the monetary authorities, work together in order to attain minimal and optimal levels of inflation to help achieve a conducive economic environment for the country.
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- Date Issued: 2020
The effects of vascular targeted bi-functionalized aunps on hepatic lipid metabolism of a diet-induced rats
- Authors: Singo, Dakalo Gladwell
- Date: 2020
- Subjects: Bile acids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47072 , vital:39780
- Description: Introduction: Obesity is a metabolic disease in which the accumulation of excessive fat in the adipose tissue may interfere with the normal maintenance of a healthy state. However, there is only a limited number of drugs approved for the treatment of obesity. For this purpose, target therapies are receiving more attention as a preferred treatment to reduce the burden of chronic diseases which are associated with the current strategies. Nanotechnology is a highly promising field that offers a nanotechnology-based drug delivery system as a novel means of solving issues associated with the toxicity of conventional anti-obesity drugs. Vascular targeted nanotherapy (targeted bi-functionalized nanoparticles) as a strategy that can overcome the current available anti-obesity drugs. Aim: This study sought to understand the effects of nanotherapy in the hepatic lipid metabolism and secretion of bile acids Research focus: The effects of gold nanotherapy on the bile acid biosynthetic enzymes, transporters and lipogenic enzymes of rats that were on a high fat diet. Conclusion: The study shows low expression in the majority of genes responsible for bile acid synthesis and a lipogenic pathway; therefore, the effects of nanotherapy exhibit low toxicity in the hepatic lipid metabolism.
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- Date Issued: 2020
Constraints in attainment of sustainable human settlements at Kwamaphumulo Local Municipality
- Authors: Sikhosana, Phindokuhle
- Date: 2020
- Subjects: Human settlements , Sustainable development
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47181 , vital:39829
- Description: This study sought to investigate the constraints in the attainment of sustainable human settlement in KwaMaphumulo Local Municipality at iLembe District in KwaZulu-Natal, South Africa. The aim of this study was to investigate the constraints that limit the achievement of the ideal of sustainable human settlement development in the KwaMaphumulo Local Municipality. Furthermore, the study sought to determine the impact of these constraints on sustainable development and to proffer measures that can be taken to combat stagnant or underdevelopment in the area. A mixed-method approach is followed in this study, which considers a triangulation of quantitative and qualitative data collection, analysis and interpretation techniques. It is important to note that in the past years since South Africa became a democratic country, there have been countless efforts with regard to overcoming the problems created during the apartheid era. These problems stemmed from a distorted spatial, social, economic and political background that was advocated by both segregationist public policies and the planning systems of the Apartheid Regime. In recent years, South African cities have also been faced with environmental issues, which are imperative to the future development of the country. With all these issues in mind, we see that the many efforts that are put in place by government always fall short of solving the problems. This research report looked at the constraints that affect the KwaMaphumulo Local Municipality in attaining sustainable human settlements and whether the efforts of redistribution and equality have been enough to overcome the problems. The key findings of the study were that constraints in attaining sustainable human settlements in the KwaMaphumulo Local Municipality negatively impacts on the development in the area. The level of services being provided is poor. There is an apparent disconnect between traditional leaders and the municipality councillor as development management and vision are concerned. Furthermore, the development programmes and projects do not seem to address the critical needs of the community. Housing development is narrow minded with a focus on the top structure and a failure to include basic services and infrastructure. Houses built that are financed by subsidies are of poor quality. The latter situation results in more able‐ bodied, skilled and capable people emigrating to developed areas.
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- Date Issued: 2020
Towards development of a malaria diagnostic: Generation, screening and validation of novel aptamers recognising Plasmodium falciparum lactate dehydrogenase
- Authors: Frith, Kelly-Anne
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Oligonucleotides , Lactate dehydrogenase , Biochemical markers , Systematic evolution of ligands through exponential enrichment (SELEX)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142247 , vital:38062
- Description: Malaria, caused by infection with the Plasmodium parasite, is one of the leading causes of death in under-developed countries. Early detection is crucial for the effective treatment of malaria, particularly in cases where infection is due to Plasmodium falciparum. There is, therefore, an enduring need for portable, sensitive, reliable, accurate, durable, self-validating and cost-effective techniques for the rapid detection of malaria. Moreover, there is a demand to distinguish between various infectious species causing malaria. Research in the area of malarial biomarkers has identified a unique, species-specific, epitope of P. falciparum lactate dehydrogenase (PfLDH), enhancing prospects for the development of diagnostics capable of identifying the species causing malarial infection. In recent years, improvements have been made towards the development of rapid diagnostic tests for detecting malarial biomarkers. Owing to their low cost, ease of labeling, and high thermal stability (relative to antibodies), the development and synthesis of aptamers that target the malarial lactate dehydrogenase represents one of the key innovations in the field of rapid diagnostics for malaria. This study explored the generation of aptamers that specifically target P. falciparum. Two sets of aptamers with diagnostically-supportive functions were generated independently, through parallel SELEX of recombinantly-expressed, full-length Plasmodium falciparum lactate dehydrogenase (rPfLDH), and an oligopeptide comprising the P. falciparum-specific epitope on lactate dehydrogenase (LDHp). The latter offers a promising solution for generating aptamers capable of binding with high specificity to P. falciparum. In this work, an rLDH class of aptamers was generated when SELEX was performed using the full-length rPfLDH protein as the target and the LDHp class of aptamers was generated when SELEX was performed using the oligopeptide LDHp as a target. Aptamers were successfully generated through the process of SELEX (systematic evolution of ligands through exponential enrichment) following the study and application of several optimisation steps, particularly during the amplification stage of SELEX. Optimisation steps included the study of improvements in PCR conditions; role of surfactants (Triton-X), modifying the PCR clean-up protocol; and agarose gel excision. Structurally-relevant moieties with particular consensus sequences (GGTAG and GGCG) were found in aptamers both reported here and previously published, confirming their importance in recognition of the target. Novel moieties particular to this work (ATTAT and poly-A stretches) were identified. Clades of consensus sequences were identified in both the rLDH and LDHp groups of aptamers, where sequences in the rLDH clade did not show preferential binding to rPfLDH while those in the LDHp clade (particularly LDHp 3 and 18) were able to recognise and bind only LDHp. Of the 19 sequences returned from the parallel SELEX procedures for rPfLDH (11 sequences) and LDHp (8 sequences), six rPfLDH and all eight LDHp sequences underwent preliminary screening and those with low responses eliminated. Of the eight LDHp-targeting aptamer sequences, five were preliminarily shown to bind to LDHp, whereas only two rPfLDH-targeting sequences were shown to bind to the target (rLDH 4 and 7). To this small selection of rPfLDH oligonucleotide sequences, two more (rLDH 1 and 15) were chosen for further study based on their sequences, secondary and predicted tertiary conformations. Sequences chosen for further study were therefore: rLDH 1, 4, 7 and 15 in the rLDH class, and LDHp 1, 3, 11, 14 and 18 in the LDHp class. Binding properties of the aptamers towards their targets were investigated using enzyme-linked oligonucleotide assays (ELONA), fluorophore-linked oligonucleotide assays (FLONA), electromobility shift assays (EMSA), surface plasmon resonance (SPR), and GelRed dissociation assays, while applications towards aptasensors were explored using electrochemical impedance spectroscopy (EIS) and fluorescent microscopy. Some inconsistencies were seen for specific aptamer to target binding interactions using specific techniques; however, generally, binding to the targets was observed across the techniques assessed. These varied responses demonstrate the need to screen and validate aptamers using a variety of techniques and platforms not necessarily specific for the proposed application. From the aptamer binding screening studies using ELONA, the most promising aptamers generated were identified as LDHp 11, rLDH 4, rLDH 7 and rLDH 15. Aptamer rLDH 4, which was generated against rPfLDH, exhibited preferential and specific binding to the lactate dehydrogenase from P. falciparum, over the recombinantly-expressed lactate dehydrogenase from Plasmodium vivax (rPvLDH), albeit with lowered responses compared to LDHp 11 in ELONA and EMSA studies. However, in kinetic ELONA studies rLDH 4 showed binding to both rPfLDH and rPvLDH. Aptamer rLDH 7 showed high affinity for rPfLDH and rPvLDH in kinetic studies using ELONA. However, screening studies with ELONA indicates that aptamer rLDH 7 may not be suitable for diagnostic tests in serum samples given its non-specific binding to human serum albumin (HSA). Aptamer rLDH 15 exhibited species specificity for rPfLDH in screening studies using ELONA but showed affinity towards rPvLDH (albeit lower relative to its affinity for rPfLDH) in kinetic studies using ELONA. LDHp 11, generated against the PfLDH peptide, showed a clear preference for rPfLDH when compared to rPvLDH and other control proteins, in both sets of ELONA studies conducted, as well as EMSA, thus possessing a strong ability to identify the presence of Plasmodium falciparum owing to its generation against the species-specific epitope. While LDHp 1 demonstrated binding to plasmodial LDH in a flow-through system (SPR), so reiterating ELONA responses, it did not perform well in the remaining methodologies. Aptamers rLDH 1 and 15 and LDHp 3, 14 and 18 exhibited a mixed set of results throughout the target protein screening analyses and were, thus, not considered for selective binding in P. falciparum parasite bodies. In studies aimed at exploring biosensor assemblies utilising the developed aptamers, both rLDH 4 and LDHp 11, along with rLDH 7, LDHp 1 and pL1, demonstrated in situ binding to the native PfLDH in fluorescent microscopy. LDHp 11 exhibited FITC-based fluorescence equivalent to the anti-rPfLDHp IgY antibody in confocal fluorescent microscopy indicating superior binding to the native PfLDH compared to the remaining aptamers. An examination of electrochemical impedance as a platform for a biosensor assembly did not, in these studies, exhibit the required sensitivity using physiologically relevant concentrations of analyte expected for pLDH following infection with Plasmodium spp. Malstat/LDH activity was explored for application in a colorimetric aptasensor. A decrease in both rPfLDH and rPvLDH activity was observed following incubation with the tested aptamers, but rLDH 1, rLDH 7 and LDHp 14 did not exhibit similar decreases in rPvLDH activity. Aptamers rLDH 1, 4 and 7 and LDHp 11 and 14 were, therefore, not selected as candidates for LDH capture in LDH activity-based diagnostic devices for P. falciparum. The decreases in pLDH activity in the presence of aptamers could hold promise as direct or antagonistic malaria therapeutic agents. Preliminary studies on the application of aptamers as malaria therapeutic agents, while of interest, should be viewed with due caution given the challenges of aptamers reaching the intracellular native plasmodial LDH hosted within the red blood cells. In conclusion, this work has shown the ability of the LDHp 11 aptamer, generated in these studies, to selectively bind rPfLDH over rPvLDH, and to bind to the native PfLDH in fluorescent microscopy, indicating that this aptamer holds promise as a biorecognition element in malaria biosensors and other diagnostic devices for the detection, and differentiation, of P. falciparum and P. vivax. The use of a species-specific epitope of P. falciparum as a target in aptamer generation paves the way for similar such studies aimed at generating aptamers with species selectivity for other Plasmodium species.
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- Date Issued: 2020
The relative efficacy of liberation theology and critical solidarity in keeping the SACC to its goals of human welfare and Ecumenical unity in its relationship with the South African state
- Authors: Pongolo, Mziwamadoda
- Date: 2020
- Subjects: Black theology Liberation theology
- Language: English
- Type: Thesis , Masters
- Identifier: http://hdl.handle.net/10353/18269 , vital:42247
- Description: The problem that this study is grappling with is that since the SACC has followed the Critical Solidarity model it is less successful in meeting its goals, to support human welfare and ecumenical solidarity in South Africa. The research will compare the two respective models of relations between SACC and State, that which guided the SACC after the demise of Apartheid that which guided it. First, the research will investigate why the present relationship of Critical Solidarity has proven less effective in achieving its goals. Secondly it will investigate factors accounting for the relative success of the model of relations between SACC and the South African State during the struggle against apartheid, that of Liberation Theology. Secondly, this study will recommend the reconstruction of a more effective model of Critical Solidarity, to remedy present weaknesses, by incorporating factors from the apartheid-era model of Liberation Theology that which serve the goals of ecumenical solidarity and human welfare. The research seeks to address the following questions. What challenges, for the goals of the SACC, of ecumenical solidarity and human welfare, have arisen for the SACC that may be attributed to its adoption of a model of Critical Solidarity in its relationship with the State? What factors conducive to ecumenical solidarity and human welfare are absent from the present model of Critical Solidarity, though they present the preceding model of relations between Church and State as adopted adopted by the SACC during the struggle against apartheid, modeled on Liberation Theology? How might these factors conducive to Church unity and social justice in South Africa be reintroduced in the present model of Critical Solidarity
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- Date Issued: 2020
A management model for human settlements: a case study of Nigeria and South Africa
- Authors: Adeniran, Adeleye Ayoade
- Date: 2020
- Subjects: Human settlements -- Nigeria , Human settlements -- South Africa Sustainable development
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/45985 , vital:39403
- Description: Persistent depreciating human settlements is a global challenge, particularly in developing countries and this challenge contributes to the continuously increasing housing gap, despite several novel policies and programmes. Although there is a dearth of research-based literature on human settlements management, studies show that property management, Facility Management, housing management and urban management impact the sustainability of all these built environment milieus which form part of the human settlements’ sub-components. This research aimed at altering the afore-said challenge by exploring the management of human settlements in Nigeria and in South Africa. Philosophically, the research is pragmatic, and it rests on positivist deductive reasoning. The methods used for data collection include a review of literature, collection of archival records, and empirical studies of purposively selected professionals and stakeholders involved in human settlements’ management in Nigeria and in South Africa. The findings indicate that existing human settlements in the case studies are “good” and “acceptable” with the majority adopting an outsourced method of management. Furthermore, the lack of maintenance records, the deficiency in policy monitoring and the training of management and maintenance personnel were the three highest ranking factors indicated to be affecting human settlements management. Moreover, an inhabitant’s income, the time available for management, professional expertise and technology employed for management are recognised as the critical sustainability sub-factors affecting human settlement management. The factor analysis established ownership, human resource and capital, social, legal, technology, economic, physical and environmental factors as vital and very significant in achieving sustainable management of human settlements. The study recommends the professionalisation of the field of human settlements as well as a formulation of management policy among others. The study developed a management model for human settlements, and it advocates that human settlements managers, owners and organisations should apply the model in this study, to improve their human settlement management practices, so as to achieve sustainability, and in essence, Sustainable Development Goal 11. It is hoped that the suggested management model will influence human settlement policy development and review, the designing of human settlement management programmes including the framework for their ongoing monitoring and evaluation. The study furthermore contributes to the development of housing and human settlement curricula and evolving human settlement research in both developed and developing countries.
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- Date Issued: 2020
Impact of change management on employee engagement
- Authors: Moche, Boitshoko
- Date: 2020
- Subjects: Personnel management Organizational change
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/49703 , vital:41772
- Description: Change is inevitable and change is necessary; in order for organisations to remain competitive they have to keep on evolving, ensuring that the organisational vision and strategy are aligned to the organisation’s vision, importantly, that the employees have a fulfilling work experience in order to do their best and live up to the organisation’s values. In the high-pressured, agile work environment, it is necessary for the organisations to move their processes with the times, providing information on digital platforms while upskilling the employees and saving time. Employee engagement is defined as the force that drives the organisational success and when achieved it eventually leads to high morale, employee retention, employee trust and loyalty to the organisation and organisational performance The aim of the study was to investigate the impact of change management process on the employee engagement in the workplace. The three identified main factors were readiness for change, process of change and leadership involvement. Change initiatives have been reported to fail by 70% if not implemented effectively and can have a negative impact on how the employees receive the change. The mixed method study approach was used, starting with interviews with the four key players in the organisation to establish how they perceive change and gather new information. It was followed by a quantitative e-survey to departments which have had technological change in the past year. The empirical results showed that the there is a relationship between the factors of change and employee engagement. Importance of readiness for change was highlighted, supporting the need for an organisational analysis followed by a detailed change plan aligned to the organisational vision. Process of change had a positive significance for employee engagement while lack of leadership involvement showed the negative relationship to employee engagement. The findings show how change leadership should work in sync with organisational leadership at all times and should incorporate change management into the organisational DNA.
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- Date Issued: 2020
A Strategy for good IT Governance in South African Municipalities
- Authors: Sibanda, Mbusi
- Date: 2020
- Subjects: Information technology -- Management -- South Africa
- Language: English
- Type: Thesis , Doctoral , DIT
- Identifier: http://hdl.handle.net/10948/49573 , vital:41737
- Description: Information technology (IT) is widely used in South African municipalities as a tool for enabling business processes and therefore its design and implementation are critical to municipalities delivering on their core mandate of delivering services to their communities. The implementation of IT relies on the adoption of sound IT governance practices, whose outcome is to ensure that municipalities derive value from the use of IT and minimise the risk associated with it. However, the implementation of IT governance has proved to be challenging in most municipalities. This research sought to devise a strategic approach to the effective and viable implementation of IT governance in municipalities in South Africa. The research was carried out by adopting a design science research approach, which is increasingly being used for studies in the IT discipline as it assists in solving problems through the design of innovative artefacts, in this case a strategy for the implementation of IT governance. To address the objectives, the research was carried out through case studies of South African municipalities which enabled a detailed investigation of phenomena in its natural context. The data was collected by means of mixed methods consisting of a literature review, a survey and semi-structured interviews. A literature review connected to the research problem was done in order to identify the present state of the issues and how they have been confronted. A survey was sent out to municipalities, institutions and individuals with a mandate to support municipalities, while semi-structured interviews were conducted in five municipalities, government departments and regulatory bodies. A thematic analysis of the data collected was performed which led to a diagnosis of the issues. These include inadequate IT infrastructure; lack of coordinated external support; inadequate IT skills; lack of strong and committed IT leadership; poor IT controls; and fragmented planning. Guiding policies and a set of coherent actions for solving the issues were developed. This culminated in the devising of a strategy for the coordinated implementation of IT governance in municipalities. The primary contribution of this study is the formulation of a strategy for the sound implementation of IT governance in South African municipalities. The research contends that by identifying the strategic issues facing municipalities and implementing the proposed actions, most of which fall outside the municipalities, a new contribution to knowledge has been made. Accordingly, the strategy facilitates the development of plans that address the root causes of the poor implementation of IT in municipalities.
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- Date Issued: 2020
Enactment of hands-on practical activities through using easily accessible resources in a Grade 10 Physical Science classroom
- Authors: Ndevahoma, Martha Kashike
- Date: 2020
- Subjects: Physical science -- Study and teaching (Secondary) -- Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/110229 , vital:33251
- Description: The Namibian National Subject Policy Guide for Physical Science Grade 8-12 (MoE, 2010) indicates that science teachers should be creative and innovative in producing their own teaching and learning support materials that are linked to practice. Practical activities play a major role in the learning of science. Due to the lack of science laboratory equipment, however, minimal practical activities are done in some schools especially under-resourced disadvantaged schools. It is against this background that this case study investigated the possibilities and challenges associated with the use of easily accessible resources to carry out hands-on practical activities. Underpinned by an interpretive paradigm, this study used a mixed-method approach by generating both quantitative and qualitative data. It was carried out in the Otjozondjupa region in Namibia at Wato Secondary School (pseudonym). It was conducted with a Grade 10 Physical Science class consisting of 30 learners. To generate data, questionnaires, focus group interviews, lesson observations, stimulated recall interviews, as well as learners’ journal reflections were used. Conceptions, dispositions, interest and sense-making were used as conceptual lenses while Vygotsky’s social constructivism was the theoretical framework that informed this study. Quantitative data were analysed using the conceptual lenses and were subsequently presented in tables and graphs. That is, a deductive approach to analysis was used. On the other hand, a thematic approach was used to analyse the qualitative data. That is, qualitative data were inductively analysed to come up with sub-themes and themes. The findings from this study revealed significant shifts in learners’ conceptions, dispositions and interest towards science after the use of easily accessible resources to do hands-on practical activities. Similarly, learners’ sense-making of science concepts improved. This study thus recommends that science teachers should, where possible, make use of easily accessible resources to carry out hands-on practical activities during their science lessons. During such hands-on practicals, however, emphasis should be on the development of scientific concepts.
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- Date Issued: 2020
Investigating the fitness of sea turtles nesting in South Africa
- Authors: Le Gouvello du Timat, Diane Z M
- Date: 2020
- Subjects: Sea turtles -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/48445 , vital:40877
- Description: The South African rookeries of the South Western Indian Ocean (SWIO) loggerhead and leatherback regional management units have been continuously monitored for more than 5 decades, representing one of the longest-running sea turtle beach protection and monitoring programmes in the world. Although such conservation has been successful in protecting adults on land when they come to nest, it might not be enough to ensure population recovery if survival is not ensured across all life stages. Sea turtles are particularly vulnerable to direct threats and changing environmental conditions because they are long-lived, slow maturing, and have low survival rates at early life stages, making them conservation-dependent. These characteristics also render them difficult to conserve as a consequence of their wide geographic distributions, spanning multiple jurisdictions and legislative systems with different conservation targets, and a complex life cycle with changes in habitats and diets at different life stages. Sea turtle populations have been severely depleted as a result of historical over-exploitation and current threats including fisheries bycatch, direct harvest, habitat loss, pollution and pathogens, and climate change. In many cases however, population declines have been reversed by a variety of effective conservation measures such as beach protection programs, and marine protected areas, enabling successful population recovery around the world. Despite protection and apparent increase in abundance, indirect pressures can still affect turtle populations. Therefore, it is fundamental to evaluate the effectiveness of conservation measures (using fitness traits such as female and hatchling sizes) and examine underlying trends. The aim of this thesis was thus to evaluate the fitness of loggerhead and leatherback sea turtles nesting in South Africa. Long-term female size trends over time were evaluated and the populations were split between first-time nesters and experienced nesters. As sea turtle reproductive output (clutch size only) is correlated with female size and evidence is showing that in some populations individual female size is decreasing, the effects of maternal body size on reproductive output and hatchling fitness were investigated for both species. Crawling and swimming speeds were used as proxies of hatchling fitness, because these two traits positively influence their survival. An oceanic model was further used in combination with a particle tracking framework to estimate, for the first time, post-hatchling dispersal of loggerhead and leatherback turtles in the SWIO. I investigated whether the trend in female size for loggerhead turtles nesting in South Africa is a result of their foraging strategy to determine if there is a foraging dichotomy (neritic and oceanic). Despite an apparent increasing population, a significant 3.7 and 3.6 cm decrease in the size of both recruit and remingrant female loggerhead turtles, but a stable mean size of leatherbacks for both age groups was observed. I hypothesise that these contradictory effects are attributable to the very distinct foraging ecologies of the two turtle species and speculate that these variations in carapace length may be driven by environmental change. The reproductive strategy of both loggerhead and leatherback turtles nesting in South Africa supports the optimal egg size theory with larger turtles producing larger clutches but not larger eggs. Egg size was thus constant irrespective of maternal length. Hatchlings with longer carapaces and flippers had higher swimming speed, suggesting that larger hatchlings are fitter (potentially higher survival) than smaller ones by spending less time in high predation risk areas (i.e. beach and nearshore). The model revealed that dispersal trajectories of both loggerhead and leatherback sea turtle hatchlings were very similar and simulations indicated that initial active swimming (frenzy) as well as variability in oceanic conditions strongly influenced dispersal of virtual hatchlings. The trends observed in this study emphasize the importance of long-term monitoring, examining not only abundance but also individual size, to understand population dynamics, support recovery planning and prioritize future conservation practices. Furthermore, the findings of this study have important implications for overall population growth if smaller hatchlings of lower fitness are produced as a result of climate change and thus have decreased dispersal abilities and lower likelihood of survival.
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- Date Issued: 2020
Non-standard employment in terms of the labour relations act
- Authors: Tatchell, Veronique
- Date: 2020
- Subjects: Labor laws and legislation -- South Africa , South Africa -- Labour Relations Act, 1995 , Flexible work arrangements -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10948/49027 , vital:41594
- Description: Historically, employers utilised non-standard or atypical forms of employment in order to avoid statutory obligations in respect of these employees, and in turn justify differential treatment of said employees.1 As a result, non-standard employees were not on the same footing as their permanent counterparts. They were not remunerated on the same level, were not privy to advancement and training opportunities, and did not enjoy a sense of job security due to the ease at which their employment could be terminated, rendering them a vulnerable class of workers.2 There was pandemonium in the employment sphere of society due to the abusive practices faced by employees employed in terms of Temporary Employment Services, this was accompanied by a call to ban labour broking. The legislature, while acknowledging the important role that this form of employment plays in the labour market and broader economy, opted for increased regulation of this and other types of non-standard employment; instead of an outright ban. As a result thereof, the Labour Relations Act3 was amended by the Labour Relations Amendment Act,4 with a view of improving the regulation and protection of employees engaged in these forms of non-standard or atypical employment. This study seeks to determine whether the amendments have achieved the purpose of enhancing the job security of these employees.
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- Date Issued: 2020
Distribution, habitat associations and conservation of the Eastern Cape rocky Sandelia bainsii (Perciform: Anabantidae) in the Great Fish River system
- Authors: Sifundza, Delsy Sindy
- Date: 2020
- Subjects: Anabantidae -- South Africa -- Great Fish River , Perciformes -- South Africa -- Great Fish River , Labyrinth fishes -- South Africa -- Great Fish River , Fishes -- Effect of human beings on -- South Africa -- Great Fish River , Fish populations -- South Africa -- Great Fish River , Fish declines -- South Africa -- Great Fish River , Fish populations -- Monitoring -- South Africa -- Great Fish River , Anabantidae -- Evolution -- South Africa -- Great Fish River , Gene flow , Mitochondrial DNA , Microsatellites (Genetics) , Eastern Cape rocky Sandelia bainsii , Freshwater ecology -- South Africa -- Great Fish River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/142692 , vital:38102
- Description: In South Africa, freshwater ecosystems are under pressure due to increasing anthropogenic factors degrading river systems. The Cape Fold, the lower Southern Temperate Highveld and the Amatolo – Winterberg Highlands freshwater ecoregions contain some of the heavily impacted river systems in the country. One such river system is the Great Fish River system, which has been affected by anthropogenic modification (hydrological modification, water pollution and invasion by non-native fish species). These modifications have raised conservation concerns on an imperilled anabantid fish, the Eastern Cape rocky Sandelia bainsii that is confined to the Kat and Koonap rivers, which are tributaries of the Great Fish River. Historically, the species was reportedly common and abundant, with a range spanning across seven river systems (Kowie, Great Fish, Keiskamma, Igoda, Gxulu, Buffalo and Nahoon rivers) in the Eastern Cape Province. However, surveys have indicated that the species has experienced decline in population size and distribution range, with localised extirpations being reported. This resulted in its listing on the IUCN Red List of threatened species as Endangered. The proposed shale gas exploration and potential infrastructure development in the Karoo basin, which encompasses the headwaters of the Kat River, represent a potential future threat to the remnant populations of this species. The aim of the present study was to review the past and present distribution range of S. bainsii, determine the habitat associations and assess the conservation of S. bainsii in the Great Fish River system. This information is essential for informing conservation and management decisions for this species in the Great Fish River system. This study undertook a comprehensive survey of the Kat and the Koonap rivers in 2017, and augmented this information with data from more recent surveys that were conducted from 2009 - 2014 to map the distributions freshwater fishes in the Great Fish River system. Data from these recent surveys (2009 – 2017) were compared with historical records (1961 – 2005) obtained from the South African Institute for Aquatic Biodiversity to evaluate changes in distribution patterns of S. bainsii. Historical records indicated that S. bainsii occurred at 11 localities in the Kat River and three localities in the Koonap River. Data from recent surveys indicated that the species has persisted at these historical localities, except one locality in the Koonap River where no individuals of S. bainsii were captured, despite the use of multiple sampling approaches and gears. Although the species still persists at two of the three historical localities in the Koonap River, the population sizes have considerably declined as the species was reportedly in high abundance at these localities in the 1980s. Factors threatening the continued existence of this species in the Koonap River likely include non-native species, extreme drought conditions and the associated excessive abstraction of water which has resulted in the drying of some of the refugia pools where the species was historically common and abundant. In contrast to the Koonap River, the 2017 survey indicated that in the Kat River, S. bainsii had a similar distribution range compared to historical records. The species occurred at 11 of the 41 localities that were sampled in the Kat River, and the sampled populations consisted of all size classes. Sandelia bainsii occurred with other native fish species in the system, including Labeo umbratus, Enteromius anoplus and Glossogobius callidus. A comparison of historical and recent data indicates that non-native fishes have likely not expanded their ranges in the Kat River. This is likely due to the presence of weirs and dams that have prevented upstream movement of non-native fish species L. aeneus, C. gariepinus and T. sparrmanii which are now abundant in the lower Kat and mainstem Great Fish River. Surveys from the present study indicated that although S. bainsii still persists within the Kat River, there are a number of factors that pose a considerable threat to the continued existence of this species in the Great Fish River system. Chapter 3 evaluated the habitat associations of S. bainsii based on 10 physical and chemical variables and distribution data collected from 30 localities in the Upper Kat River and seven of its tributaries. A non-metric multidimensional scaling model was used to assess the distribution patterns of S. bainsii in relation to environmental variables. Furthermore, a negative binomial generalised linear model (GLM) was used to assess the relationship between the abundance of S. bainsii and environmental variables. Results from the present study indicated that S. bainsii was a habitat generalist as the distribution of this species showed no specific association with any of the habitat variables that were considered in this study. The species occurred in a wide range of habitats, including rocky sections in the upper Kat River and its tributaries as well as the mainstem sections. The GLM however indicated that the abundance of S. bainsii was negatively associated with increasing conductivity. In addition, the Kat River contains multiple weirs of variable sizes. Future studies should therefore evaluate the effects of these weirs on population fragmentation, gene flow and long-term evolutionary potential of S. bainsii through the application of techniques such as mitochondrial DNA and microsatellites.
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- Date Issued: 2020
Examining the effect of Diversity, Inclusion and Equity on Work Engagement and Innovation on South African Organisations
- Authors: Efalao, D M
- Date: 2020
- Subjects: Organizational effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/48202 , vital:40520
- Description: The biggest strengths of organisational success lie in its employees; therefore, employee engagement is a fundamental force that is linked to organisational effectiveness and success. The level of employee engagement in South Africa according to the polls remains very low. On the other front, innovative ideas prove to be a vital element of organisational innovativeness and progress. Today’s business environment is unstable and to remain relevant in the marketplace, it is imperative for organisations to embrace innovation and become responsive to changing customer needs. South Africa’s population is very diverse coming second in diversity after the United States. The diversity of South Africa as a national endowment could be investigated to see if it could be leveraged to improve organisational innovation and effectiveness through work engagement. Being mindful of the fact that being diverse is not equal to being inclusive, inclusion is also investigated to see its effect on work engagement and organisational innovation. The principle of fairness (equity) was also investigated with relation to engagement and innovation. The purpose of the study is to assist organisations to improve employee engagement and organisational innovation by taking advantage of the national factor endowment of diversity and inclusion. Equity is investigated as an important principle that could possibly have an effect on employee engagement or organisational innovation. The findings were that diversity and inclusion have a significant relationship with employee engagement and organisational innovativeness while equity has a significant relationship with organisational innovativeness. Recommendations were therefore made to leaders of the organisations to embrace workforce diversity, inclusion and fairness in organisational structures and practices in order to realise improved employee engagement and organisational innovativeness.
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- Date Issued: 2020
Water balance processes in indigenous and introduced spaces three production system in the Southern Cape region of South Africa
- Authors: Mapeto, Tatenda
- Date: 2020
- Subjects: Hydrology -- Research -- South Africa , Groundwater -- Research -- South Africa Trees -- Breeding Sustainable forestry
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/49325 , vital:41621
- Description: Forests and trees are important modulators of water flow and their position in the landscape impacts the spatial and temporal availability of ground water and surface water resources. As such, the global extent of natural forests is linked to the availability of water. In the circumstances where tree production systems have been introduced and are managed in landscapes previously populated with other forms of vegetation, it is necessary to understand their hydrological impact so as to ensure that the benefits derived from these tree systems do not outweigh the costs of water availability for sustaining human and environmental water requirements.
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- Date Issued: 2020