Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions
- Authors: Hennessy, Fritha , Nicoll, Willam S , Zimmerman, Richard , Cheetham, Michael E , Blatch, Gregory L
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6487 , http://hdl.handle.net/10962/d1006270 , http://dx.doi.org/10.1110/ps.051406805
- Description: Heat shock protein 40s (Hsp40s) and heat shock protein 70s (Hsp70s) form chaperone partnerships that are key components of cellular chaperone networks involved in facilitating the correct folding of a broad range of client proteins. While the Hsp40 family of proteins is highly diverse with multiple forms occurring in any particular cell or compartment, all its members are characterized by a J domain that directs their interaction with a partner Hsp70. Specific Hsp40-Hsp70 chaperone partnerships have been identified that are dedicated to the correct folding of distinct subsets of client proteins. The elucidation of the mechanism by which these specific Hsp40-Hsp70 partnerships are formed will greatly enhance our understanding of the way in which chaperone pathways are integrated into finely regulated protein folding networks. From in silico analyses, domain swapping and rational protein engineering experiments, evidence has accumulated that indicates that J domains contain key specificity determinants. This review will critically discuss the current understanding of the structural features of J domains that determine the specificity of interaction between Hsp40 proteins and their partner Hsp70s. We also propose a model in which the J domain is able to integrate specificity and chaperone activity.
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- Date Issued: 2005
How policy discourses and contextual realities influence environmental teaching and learning processes in early childhood development: a case study of the Raglan Road child care centre
- Authors: Vallabh, Priya
- Date: 2005
- Subjects: Early childhood education -- South Africa , Health education -- South Africa , Environmental education -- Study and teaching -- South Africa , Day care centers -- South Africa , Child development
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1559 , http://hdl.handle.net/10962/d1003441 , Early childhood education -- South Africa , Health education -- South Africa , Environmental education -- Study and teaching -- South Africa , Day care centers -- South Africa , Child development
- Description: This case study considers the relationship between context, school policy and environmental teaching and learning processes at a community-based early childhood development centre in South Africa. The study recognises that educational practices in the early childhood development field are shaped by historical, cultural, economic and political realities at both local and national levels. It is from the understanding that each school is a unique composition of these shaping factors that the research was designed to consider the community-based school participating in this study. By compiling a contextual profile, this study attempts to consider dominant contextual factors affecting the school. Through the critical discourse analysis of a school policy document, this study considers local level policy, and through the literature chapter, national policy. Teacher interviews provide insight into teacher understanding of school policy in response to contextual issues, as well as providing insight into how teachers perceive their translation of policy into teaching practice. Observations of lessons in the centre provided an. opportunity to see how context and policy translated into and influenced environmental teaching and learning processes. This study looks at how environmental education is addressed in the Raglan Road Child Care Centre, and provides insight into how environmental education within the context of the school and in relation to school policy may be strengthened. It comments on the tensions and ambivalences arising from the relationships between context, policy and environmental teaching and learning processes and makes recommendations to address these ambivalences in ways that are contextually relevant. The main recommendations were designed to be practically useful for the school involved in the study and are focused around engaging the ambivalences emerging from this study to open up 'spaces' for deliberating environmental teaching and learning processes and other tensions arising out of the study at an ECD level. Recommendations included: 1) engaging with the strong development focus in school policy and the educational focus in national policy and teacher discourse; 2) deliberating the ways in which school policy and national policy respond to risk; 3) engaging with the ambivalence in the school-parent relationship; 4) the re-alignment of the explicit curriculum and broadening the contextually-based view of whole child development; and 5) engaging the ambivalence in approaches to education at the centre.
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- Date Issued: 2005
Burrow utilization by springhares (Pedetescapensis) in the Eastern Cape, South Africa
- Authors: Peinke, D M , Brown, C R
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6962 , http://hdl.handle.net/10962/d1012025
- Description: The use of burrows by springhares was investigated in the Eastern Cape, South Africa using radio telemetry. Springhares utilized 4–27 different burrows scattered over areas ranging from 0.6–28.5 ha, with maximum distances of 170–1000 m between burrows. Springhares frequently changed burrows, seldom spending more than a few consecutive days in each, unless rearing young. The number of burrows used by individuals was correlated with the length of time they were tracked and, even after a year, some individuals were still occupying burrows previously unused by them. Males and females did not differ significantly in the mean number of burrows used, the mean area over which these were distributed, or the mean maximum distance between burrows. Springhares were generally solitary and occupied burrows were, with few exceptions, apparently avoided by conspecifics.
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- Date Issued: 2005
Institutional change and ecosystem dynamics in the communal areas around Mt Coke State Forest, Eastern Cape, South Africa
- Authors: Cundill, Georgina
- Date: 2005
- Subjects: Biodiversity -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape , Natural resources -- Management -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Nature conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:4746 , http://hdl.handle.net/10962/d1006959 , Biodiversity -- South Africa -- Eastern Cape , Ecosystem management -- South Africa -- Eastern Cape , Natural resources -- Management -- South Africa -- Eastern Cape , Sustainable agriculture -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Nature conservation -- South Africa -- Eastern Cape
- Description: Through a combination of theoretical discussion and case study analysis from two villages in the Eastern Cape Province, South Africa, this thesis explores the relationship between institutional change and ecosystem dynamics through a multi-scale approach that combines local and scientific knowledge. Several conceptual approaches were combined in this study. These included; the Resilience perspective, the Sustainable Rural Livelihoods approach, the Millennium Assessment framework and principles, and the Environmental Entitlements approach. Various participatory research techniques were employed which combined with GIS technologies, existing data sets, and historical records. Common pool resource areas are social spaces, where local values attached to resources are institutionally mediated, politically nuanced, economically interpreted and historically situated. Political driving forces at various scales have played a disproportionate role in local level institutional functioning in the case study area. In particular, inappropriate state-lead interventions into land use planning have weakened local level institutions, and have reduced the ability of the linked social-ecological system to cope with change and uncertainty. People and ecosystems become more vulnerable when driving forces such as political upheaval, economic depression and drought over-lap. However, rural people are not mere spectators in the face of these driving forces; they respond both reactively and proactively to ensure resilience to change and uncertainty. Ecosystems at Mt Coke play a key role during times of crisis in rural livelihoods. These ecosystems have undergone various short-term cyclical changes largely in response to rainfall fluctuations, and some longer-term changes linked to political events and trends that have affected management practices and local institutions over time. Orthodox ecological interpretations of ecosystem change appear to ignore four key factors identified in this study: 1) the role of institutions in shaping access to resources, 2) the demand for resources in rural livelihoods, 3) the dynamic interaction between social and natural systems, and 4) the interaction between social and natural systems across scales of analysis. The future of common pool resource management lies in the combination of local and scientific knowledge through an adaptive management approach that encourages learning and adaptation in local level institutional structures.
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- Date Issued: 2005
Stress-inducible protein 1: a bioinformatic analysis of the human, mouse and yeast STI1 gene structure
- Authors: Aken, Bronwen Louise
- Date: 2005
- Subjects: Molecular chaperones , Proteins -- Analysis , Heat shock proteins , Bioinformatics , Genetics -- Data processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3990 , http://hdl.handle.net/10962/d1004049 , Molecular chaperones , Proteins -- Analysis , Heat shock proteins , Bioinformatics , Genetics -- Data processing
- Description: Stress-inducible protein 1 (Sti1) is a 60 kDa eukaryotic protein that is important under stress and non-stress conditions. Human Sti1 is also known as the Hsp70/Hsp90 organising protein (Hop) that coordinates the functional cooperation of heat shock protein 70 (Hsp70) and heat shock protein 90 (Hsp90) during the folding of various transcription factors and kinases, including certain oncogenic proteins and prion proteins. Limited studies have been conducted on the STI1 gene structure. Thus, the aim of this study was to develop a comprehensive description of human STI1 (hSTI1), mouse STI1 (mSTI1), and yeast STI1 (ySTI1) genes, using a bioinformatic approach. Genes encoded near the STI1 loci were identified for the three organisms using National Centre for Biotechnology Information (NCBI) MapViewer and the Saccharomyces Genome Database. Exon/intron boundaries were predicted using Hidden Markov model gene prediction software (HMMGene) and Genscan, and by alignment of the mRNA sequence with the genomic DNA sequence. Transcription factor binding sites (TFBS) were predicted by scanning the region 1000 base pairs (bp) upstream of the STI1 orthologues’ transcription start site (TSS) with Alibaba, Transcription element search software (TESS) and Transcription factor search (TFSearch). The promoter region was defined by comparing the number, type and position of TFBS across the orthologous STI1 genes. Additional putative TFBS were identified for ySTI1 by searching with software that aligns nucleic acid conserved elements (AlignACE) for over-represented motifs in the region upstream of the TSS of genes thought to be co-regulated with ySTI1. This study showed that hSTI1 and mSTI1 occur in a region of synteny with a number of genes of related function. Both hSTI1 and mSTI1 comprised 14 putative exons, while ySTI1 was encoded on a single exon. Human and mouse STI1 shared a perfectly conserved 55 bp region spanning their predicted TSS, although their TATA boxes were not conserved. A putative CpG island was identified in the region from -500 to +100 bp relative to the hSTI1 and mSTI1 TSS. This region overlapped with a region of high TFBS density, suggesting that the core promoter region was located in the region approximately 100 to 200 bp upstream of the TSS. Several conserved clusters of TFBS were also identified upstream of this promoter region, including binding sites for stimulatory protein 1 (Sp1), heat shock factor (HSF), nuclear factor kappa B (NF-kappaB), and the cAMP/enhancer binding protein (C/EBP). Microarray data suggested that ySTI1 was co-regulated with several heat shock proteins and substrates of the Hsp70/Hsp90 heterocomplex, and several putative regulatory elements were identified in the upstream region of these co-regulated genes, including a motif for HSF binding. The results of this research suggest several avenues of future experimental work, including the confirmation of the proposed core promoter, upstream regulatory elements, and CpG island, and the investigation into the co-regulation of mammalian STI1 with its surrounding genes. These results could also be used to inform STI1 gene knockout experiments in mice, to assess the biological importance of mammalian STI1.
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- Date Issued: 2005
A hermeneutic description of a therapeutic interview using reader response concepts from literary theory
- Authors: Odendaal, Dirk Hermanus, 1954-
- Date: 2005
- Subjects: Psychotherapy , Hermeneutics , Narrative therapy , Reading, Psychology of , Reader-response criticism , Psychology and literature
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3168 , http://hdl.handle.net/10962/d1007749
- Description: Certain approaches in the discipline Psychology, use the term narrative to describe how they work. Upon investigation one finds that the term narrative is seldom informed from Literary Theory, the background from which it originated. Instead, other disciplines that were also influenced by Literary Theory are invariably used as a means of cross fertilisation, e.g. the work of Geertz from an anthropologist background. Therapists make use of techniques described in the theories in an attempt to come to an understanding of the interactions in the therapy session. Some of the later theories emanating from Literary Theory appear to very useful for opening new ways of research in psychology, especially because some of them already come from an interdisciplinary background. This research attempts to identify useful theories and then apply them within a hermeneutical background in a therapeutical session. Theoretical work on ambiguity, recent research on foregrounding and defamiliarization and also the research in psychonarratology appear to be eminently useful for coming to a deeper understanding of the processes that take place in a therapeutic environment. It is thought that these theories could be of use because they have been 'tested' against the experiences of real readers reading texts. As novels differ from reports and washing lists, therapeutic settings differ from discussions. A novel is a cultivated variant of a report, and a therapeutic conversation is a cultivated version of a chat. These theories then, were applied to a real therapeutic session. The therapists who participated were interviewed on the session and on their reactions to certain 'readings' made by them during the session. The purpose of the interview was to obtain an understanding of their interpretation of what had happened during that session. The questions, reactions, observations and reflections of the session constitute the text of this research. The generated text was then reread from the perspective of each of the theories. The data was collected and interpreted. The interpretation focusses on the therapists 'reading' or understanding of the session and in the process, leads the therapists and researcher to further levels of understanding. In conclusion, it was found that the theories were indeed useful as they were able to point out how certain stylistics of language and situation in the therapeutic session had led to hermeneutic or interpretive processes and also how these processes were perceived or experienced on reflection by the therapists.
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- Date Issued: 2005
Reproductive biology of a riverine cyprinid, Labeo umbratus (Teleostei: Cyprinidae), in small South African reservoirs
- Authors: Potts, Warren M , Booth, Anthony J , Hecht, Thomas , Andrew, Timothy G
- Date: 2010
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125854 , vital:35826 , https://doi.10.2989/16085910509503849
- Description: The reproductive and recruitment characteristics of moggel, Labeo umbratus, populations were examined in four small South African reservoirs. Reproduction, characterised by an extended spawning season, high fecundity, short incubation time and rapid larval development, appears to be ideally suited to the highly variable environment of small reservoirs. Evidence suggested that L. umbratus spawns in the reservoirs. In two reservoirs where samples were conducted monthly, GSI (gonado-somatic index) was positively correlated with both water temperature and day length, whilst the CPUE (catch per unit effort) of juveniles was not related to any environmental variable. The success of moggel spawning appeared to increase when there was early spring and consistent summer rainfall.
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- Date Issued: 2010
The feeding ecology and habitat use of lions reintroduced to small, enclosed reserves in the Eastern Cape Province, South Africa
- Authors: Rapson, Jackie Anne
- Date: 2005
- Subjects: Lion -- Food -- South Africa -- Eastern Cape , Lion -- Behavior -- South Africa -- Eastern Cape , Predation (Biology) , Lion -- Nutrition
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5938 , http://hdl.handle.net/10962/d1018268
- Description: Lions have recently been introduced to a number of small (<300km²), enclosed reserves in the Eastern Cape Province as a draw-card for tourists. However, there have been few studies of lion behaviour or predator prey interactions within small reserves. Thus the aim of this study is to contribute towards a better understanding of the effects of space on diet and habitat use of re-introduced lions. The research was conducted at two small private reserves, Shamwari and Kwandwe. Kills were mostly large (55%, >120kg), adult (41%) and male (39%) and the five most commonly killed species (kudu, black wildebeest, warthog, bushbuck and Blesbuck) comprised 67.9% of all kills and 67.8% of total biomass consumed. In an attempt to overcome the problem of gaps in the kill lists, a subset of data, which excluded kills more than five days apart, was analyzed and this gave similar results to analysis of the full kill lists. Mean daily intake rates varied annually between 2.26 and 5.23kg.FEQ⁻¹ for the full data set and 4.53 and 1 0.46kg.FEQ⁻¹ for the subset. The difference between estimated (calculated using daily intake rates of 5 and 7.12kg.FEQ⁻¹) and observed offtake for each ungulate species reflected the preference ratings for that species. Analysis of bone marrow indicated that: lions killed ungulates in moderate to good condition; condition of culled animals was lower than that of kills; distal bones could be used in analyses; and that that bones exposed to the environment for at least a week could be used to analyze condition of prey. Home ranges and activity centers ranged from 21.42km² to 73.27km² and 2.7lkm² to 17.47km² respectively, which was smaller than expected from previous studies. Although, lions at K wandwe and Shan1wari preferred different vegetation types, at both reserves they generally occupied areas that were structurally similar to what has previously been reported. Continuous monitoring and analyses of diet, including condition of the prey, and habitat use will provide reserve managers with some of the necessary information to enable them to effectively manage their lion populations and to predict and minimize potential problems.
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- Date Issued: 2005
Development of an experimental system to investigate the interaction between the Helicoverpa armigera stunt virus capsid protein and viral RNA
- Authors: Nel, Andrew James Mascré
- Date: 2005
- Subjects: Helicoverpa armigera , RNA viruses
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3946 , http://hdl.handle.net/10962/d1004005 , Helicoverpa armigera , RNA viruses
- Description: Tetraviruses are entomopathogenic viruses that propagate solely in lepidopteran hosts. Viruses of this group possess non-enveloped 38- to 40-nm capsids arranged in T = 4 surface symmetry. The viral genome consists of one or two single stranded positive sense RNA strands, which define the two genera of this family, the monopartite betatetraviruses and the bipartite omegatetraviruses. Two extensively studied members of the tetraviruses are the omegatetraviruses, Helicoverpa armigera stunt virus (HaSV) and the closely related Nudaurelia capensis ω virus (NωV). The larger genomic strand of HaSV (RNA1) encodes the viral replicase, while the other (RNA2) encodes the 71-kDa capsid precursor protein (p71). The pro-capsid is assembled from 240 copies of p71, which undergo a maturation auto-catalytic cleavage into the 64-kDa (p64) capsid protein and a 7-kDa peptide (p7) forming the capsid shell. The mechanism for the recognition and packaging of the viral genome is poorly understood for these viruses. The principle objective of the research described in this study was to develop in vitro and in vivo experimental systems to investigate interactions between the N terminal domain of HaSV p71 and viral RNAs. More specifically, the two positively charged clusters of predominantly arginine residues that are conserved amongst tetraviruses and the structurally analologous nodaviruses capsid protomers’ N terminal domains were investigated. An in vitro RNA-protein “pull down” system was developed using the rapid protein purification technique of the IMPACTTM-CN system. The coding sequence of the N terminal domain of p71 was fused to that of a chitin binding affinity tag (intein). This fusion protein was used as protein bait for the viral RNA. It was proposed that if RNA interacted with the fusion protein, it would be pulled down by the mass of affinity matrix and be precipitated and fluoresce when analysed by agarose gel electrophoresis using ethidium bromide. Despite optimisation of the in vitro assay, results were affected by the interaction between the intein-tag and nucleic acids, the state of the expressed fusion protein (in particular self-cleavage) and the excessive fluorescence present on the gels. The ADH2-GAPDH yeast expression system was used to investigate the in vivo assembly of p71 containing deletions of either one or both clusters within N terminal domain. It was found that all p71 mutants were expressed with the exception of the mutant containing a deletion of the second cluster. The reasons for this still require further investigation. The expressed p71 mutants were not processed into p64 and were degraded in vivo. In addition, an experimental attempt to purify assembled p71 mutant VLPs was unsuccessful. The assembly defect of p71 mutants emphasised the significance of the clusters, which are possibly required for interaction with viral RNAs for efficient VLP assembly. The results of this study suggest that an alternative tag or in vitro RNA-protein interaction assay be used. In addition, further experiments are required to investigate whether the co-expression of full length viral RNAs are required to rescue the in vivo assembly defect of p71 mutants into VLPs.
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- Date Issued: 2005
An exploratory study of inclusion of learners with visual impairment into mainstream schools : the learners' parents' and educators' perspectives
- Authors: Ngxata, Ncediwe Gratia
- Date: 2005
- Subjects: Blind -- Education -- South Africa -- Eastern Cape Blind children -- Education -- South Africa -- Eastern Cape Children with visual disabilities -- South Africa -- Eastern Cape Mainstreaming in education -- South Africa -- Eastern Cape People with visual disabilities -- Education -- South Africa -- Eastern Cape Inclusive education -- South Africa -- Eastern Cape Special education -- South Africa -- Eastern Cape Parents of children with disabilities -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:5954 , http://hdl.handle.net/10962/d1008096
- Description: The study explores the experiences of inclusion of learners with visual impairment into mainstream schools in Mdantsane and Duncan Village in the Eastern Cape. The learners previously attended a special school away from home for the visually impaired in Port Elizabeth. The study delves into the barriers to inclusion as identified by the parents, educators and learners. A study of this nature was essential as many learners with visual impairment, are sent away to special schools on the grounds that they are unfit to attend mainstream schools when they could do well in mainstream schools when provided with appropriate support. As inclusive education is fairly new in South Africa, studies of this kind provide the Department of Education with some understanding of what the situation is with regard to the implementation of inclusive policies. The study attempts to establish from the participants what they think inclusive education is, the reason for introducing inclusive education, the barriers to inclusive education and the kind of support required to implement inclusive education in South Africa. Data collection was through semi-structured interviews and focus groups. A sample of educators, learners and parents was selected from two high schools and one primary school. Coding was used in data reduction and analysis. The study revealed that some learners, educators and parents are not totally against inclusive education as long as they could be provided with the necessary support. The study also revealed the excitement of learners and parents about attending nearby schools which are close to their homes. However two parents and learners are not satisfied due to lack of support and would rather go back to special school. The study ends with recommendations about how inclusive education could be intensified to ensure that learners with disabilities benefit from the mainstream schools. There is no doubt that this research study will be a strong contribution towards inclusive education initiatives particularly with regard to learners with visual impairment in the Eastern Cape Province.
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- Date Issued: 2005
Talk radio and public debate : a case study of three Ugandan radio stations
- Authors: Ogoso, Erich Opolot
- Date: 2005
- Subjects: Radio stations -- Uganda , Radio broadcasting -- Social aspects -- Uganda , Radio broadcasting -- Political aspects -- Uganda , Radio talk shows -- Uganda , Interviewing on radio -- Uganda
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3514 , http://hdl.handle.net/10962/d1007723 , Radio stations -- Uganda , Radio broadcasting -- Social aspects -- Uganda , Radio broadcasting -- Political aspects -- Uganda , Radio talk shows -- Uganda , Interviewing on radio -- Uganda
- Description: This study is a comparative examination of approaches to talk radio as a genre on three Ugandan radio stations. The aim is to draw conclusions, from observations made about these stations, about the potential of talk radio to encourage public debate around social issues and improve democratic participation despite pertinent challenges in Uganda. The study first outlines a theoretical framework, which is informed by Habermas's theory of the media as a 'public sphere'. This framework is applied to an exploration of traditions of talk radio that have emerged globally in order to assess the potential of these traditions to play a role in contributing to the establishment of such a 'public sphere'. The study then goes on to discuss the historical development of radio in Uganda and the establishment of the current broadcast landscape. The focus is on the way in which this history has been defined by a struggle around public expression, in which government has repeatedly sought ways to control media as a vehicle for public expression. It is proposed that Ugandan talk radio has the potential to play an important role in ensuring broad participation in public expression. It is against this background that the study then describes and analyses the development of the talk genre at three Ugandan radio stations (each one an example of, respectively, a commercial, community and public service station). It is explained that staff on all three stations emphasise the importance of talk radio in encouraging participation, by their audiences, in the public debate of social and political issues. It is argued that, because of limitations that exist within these stations, none of the talk show teams fully realize the potential of the genre for participation in such debate. The picture that emerges is one of unequal access, with those sections of radio audiences in positions of privilege being further empowered, while those on the margins remain excluded from public discussion. The study finally recommends ways to improve public participation on Ugandan talk radio, noting the need to review government support, the problems of organizational culture within the stations, the need for more guidelines on practical arrangements around talk show production and the question of contradictions that exist at policy level.
- Full Text:
- Date Issued: 2005
Regional development in South Africa’s West Coast: 'dividends on the process side'?
- Authors: Bek, David , Binns, Tony , Nel, Etienne L
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6674 , http://hdl.handle.net/10962/d1006648
- Description: Academics, policy-makers and consultants have been increasingly focusing upon the 'region' as the crucible of economic development. This regionalist approach places institutions at the centre of the process of stimulating growth. Much debate has been ongoing in academic circles, particularly those inhabited by economic geographers, about the veracity of the regionalist approach and the explanatory power of the terminology associated with it. This paper analyses explicit efforts to engender regional economic development in the West Coast of South Africa. Our empirical evaluation indicates that policy actors have sought to upgrade the institutional infrastructure of the region and that some significant achievements are evident within this realm. However, these achievements are yet to be associated with sustained regional economic takeoff. The paper concludes by asking searching questions about the ability of the regionalist approach to deliver meaningful socio-economic transformation.
- Full Text:
- Date Issued: 2005
Implementing the CoSaWoE models in a commercial workflow product
- Authors: Erwee, Carmen
- Date: 2005
- Subjects: Computers -- Access control , Workflow , Computer security , Data protection
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:9732 , http://hdl.handle.net/10948/169 , Computers -- Access control , Workflow , Computer security , Data protection
- Description: Workflow systems have gained popularity not only as a research topic, but also as a key component of Enterprize Resource Planning packages and e- business. Comprehensive workflow products that automate intra- as well inter-organizational information flow are now available for commercial use. Standardization efforts have centered mostly around the interoperability of these systems, however a standard access control model have yet to be adopted. The research community has developed several models for access control to be included as part of workflow functionality. Commercial systems, however, are still implementing access control functionality in a proprietary manner. This dissertation investigates whether a comprehensive model for gain- ing context-sensitive access control, namely CoSAWoE, can be purposefully implemented in a commercial workflow product. Using methods such as an exploratory prototype, various aspects of the model was implemented to gain an understanding of the di±culties developers face when attempting to map the model to existing proprietary software. Oracle Workflow was chosen as an example of a commercial workflow product. An investigtion of the features of this product, together with the prototype, revealed the ability to affect access control in a similar manner to the model: by specifying access control constraints during administration and design, and then enforcing those constraints dynamically during run-time. However, only certain components within these two aspects of the model directly effected the commercial workflow product. It was argued that the first two requirements of context-sensitive access control, order of events and strict least privilege, addressed by the object design, role engineering and session control components of the model, can be simulated if such capabilities are not pertinently available as part of the product. As such, guidelines were provided for how this can be achieved in Oracle Workflow. However, most of the implementation effort focussed on the last requirement of context-sensitive access control, namely separation of duties. The CoSAWoE model proposes SoD administration steps that includes expressing various business rules through a set of conflicting entities which are maintained outside the scope of the workflow system. This component was implemented easily enough through tables which were created with a relational database. Evaluating these conflicts during run-time to control worklist generation proved more di±cult. First, a thorough understanding of the way in which workflow history is maintained was necessary. A re-usable function was developed to prune user lists according to user involvement in previous tasks in the workflow and the conflicts specified for those users and tasks. However, due to the lack of a central access control service, this re- usable function must be included in the appropriate places in the workflow process model. Furthermore, the dissertation utilized a practical example to develop a prototype. This prototype served a dual purpose: firstly, to aid the author's understanding of the features and principles involved, and secondly, to illustrate and explore the implementation of the model as described in the previous paragraphs. In conclusion the dissertation summarized the CoSAWoE model's compo- nents which were found to be product agnostic, directly or indirectly imple- mentable, or not implemented in the chosen workflow product. The lessons learnt and issues surrounding the implementation effort were also discussed before further research in terms of XML documents as data containers for the workfow process were suggested.
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- Date Issued: 2005
An organisation development intervention in a previously disadvantaged school in the Eastern Cape
- Authors: Mitchell, Pauline
- Date: 2005
- Subjects: Educational change -- South Africa -- Eastern Cape Education and state -- South Africa -- Eastern Cape Education -- Social aspects -- South Africa -- Eastern Cape Education -- South Africa -- Eastern Cape School improvement programs -- South Africa -- Eastern Cape -- Case studies Education -- Aims and objectives -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1682 , http://hdl.handle.net/10962/d1003565
- Description: “We often spend too much time coping with problems along our path that we forget why we are on that path” Peter Senge This study describes and analyses the implementation of Organisation Development (OD) to a previously disadvantaged school. OD is a relatively new method of planned change in South Africa. Unlike more traditional change initiatives, OD promotes collaboration; it tries to involve all members of an organisation in problem solving and decision-making. It is an applied behavioural science discipline dedicated to improving organisations and the people in them. Previously disadvantaged schools in South Africa continue to be disadvantaged. Ten years after the introduction of democracy there have been few changes in some of these schools and some seem to be getting worse. This study was an attempt to introduce a process of planned change to one such school. Since 1994 many changes have been imposed on our schools with new curricula, increased class sizes, changes in systems of assessment and teaching methods and the abolishment of past procedures such as corporal punishment. Teachers have had little say in any of these changes and this has resulted in resistance, resignation, frustration and in many cases a lack of ability to cope. OD was introduced to Acacia High School in the form of a Survey Data Feedback (SDF). An action research process followed and a diagnosis was made followed by action planning and then the execution of a plan. My study follows this process and the implementation of the plan describing its successes. Sadly change was not sustained and I highlight some of the challenges that face the school in order to bring about real long-term improvement in the culture of learning and teaching.
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- Date Issued: 2005
Trauma, imagery and the therapeutic relationship : Langu's story
- Authors: Karpelowsky, B , Edwards, D J A
- Date: 2005
- Language: English
- Type: Article
- Identifier: vital:6268 , http://hdl.handle.net/10962/d1008267
- Description: This paper, a phenomenological case study, describes the psychotherapy of Langu (pseudonym), a 21-year-old student, who presented with Acute Stress Disorder following a series of motor accidents that affected him and his family. Langu's most distressing experience was having to identify his brother's mutilated and severely burned body. Because of the intensity of the intrusive re-experiencing of traumatic imagery and the degree of dissociative numbing, Langu participated in four intensive guided imagery sessions, which involved reliving the incident, and imaginal dialogues with his dead brother. Session records and supervision notes from the therapy process that unfolded over 22 sessions served as the basis for a thematically selective case narrative. Additional material was obtained from several research interviews with Langu over the following months. The narrative highlights the impact of the imagery work as well as relational aspects of the therapy. The case narrative provides a source for examining many aspects of the psychological impact of trauma and the path to healing, as well as the dilemmas and challenges faced by therapists working with traumatised individuals.
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- Date Issued: 2005
Synthesis and photophysical properties of a covalently linked porphyrin-phthalocyanine conjugate
- Authors: Zhao, Zhixin , Ogunsipe, Abimbola O , Maree, M David , Nyokong, Tebello
- Date: 2005
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/300358 , vital:57922 , xlink:href="https://doi.org/10.1142/S1088424605000253"
- Description: The synthesis of a phthalocyanine-porphyrin heteropentamer (zinc(II) tetra(5-phenoxy-10,15,20-triphenylporphyrin)) zinc(II) phthalocyanine, (ZnPc-(ZnTPP)4), containing four units of zinc tetraphenylporphyrin linked to a central zinc phthalocyanine macrocycle via an ether linkage is reported. The photophysical parameters of the pentamer are reported in toluene and dimethyl sulfoxide (DMSO). The observed differences in the fluorescence behavior of the pentamer in the two solvents is explained in terms of emission from different states; charge transfer state in DMSO and locally excited state in toluene. The rate constants for fluorescence, intersystem crossing, internal conversion, and of charge and energy transfer are reported for the pentamer. Quantum yields for fluorescence, internal conversion, triplet state and of charge and energy transfer are also reported for the pentamer, ZnPc-(ZnTPP)4 and the mixture of ZnPc and ZnTPP. The latter two parameters are higher in the pentamer compared to a mixture containing ZnPc and ZnTPP.
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- Date Issued: 2005
The development of an integrated management model for occupational health and safety in medical institutions
- Authors: Du Toit, Willem Johannes
- Date: 2005
- Subjects: Industrial safety -- South Africa -- Management , Industrial hygiene -- South Africa -- Management , Health facilities -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8551 , http://hdl.handle.net/10948/1300 , Industrial safety -- South Africa -- Management , Industrial hygiene -- South Africa -- Management , Health facilities -- South Africa
- Description: Health and safety management forms part of the overall risk management of medical institutions, and deals with the responsibility of an organisation to provide a risk free environment for all who are exposed to the activities of such medical institutions. Affected people include health care workers, contractors, visitors, and patients. Medical institutions, and hospitals specifically, are uniquely distinct from other industries in that they employ highly skilled staff with specific specialised knowledge. These institutions are also increasingly making use of complex technology that requires specialist staff to operate medical machines and equipment. These specific aspects are accounted for in this research. The overall purpose of this research is to determine the need for an alternative approach to the management of health and safety in medical institutions. An appropriate model will be developed that can be integrated into the existing health and safety management system. The research methodology for this study comprises the following steps: Firstly, the health and safety environment was researched in relation to all stakeholders, external and internal, that are affected by medical institutions’ activities. The effect of these activities, and how it influences health and safety management, was evaluated in a South African context. Secondly, the overall risk management approach of medical institutions and the effect of organisational culture were researched to determine the effect on health and safety performance. Thirdly, a questionnaire was sent to selected staff of five hospitals in the Eastern and Western Cape to determine their perception and experience of health and safety management. Quantitative data on incidents in Netcare Hospitals were obtained, and a comparison of the questionnaire studies was done to determine the need for a health and safety model. The final step of this study entailed the development of a health and safety model using legislative requirements and the needs identified in the research. A proposal is made to integrate specialist functions and departments into the existing health and safety management system, in order to strengthen the managerial capabilities of such a system. The importance of improving the safety and compliance culture is indicated.
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- Date Issued: 2005
Research portfolio
- Authors: Shihako, Mathilde
- Date: 2005
- Subjects: Rundu College of Education (Namibia) Education -- Namibia Educational change -- Namibia Curriculum planning -- Namibia Teaching -- Namibia Teachers -- Training of -- Namibia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1729 , http://hdl.handle.net/10962/d1003612
- Description: This portfolio discusses various important aspects that affect teacher education. The portfolio has become an important tool for assessment in education. The research component of the masters' course that I attended is reflected in this portfolio. To complete this course several aspects were covered. The educational reform process in the Namibian context requires the use of appropriate resources, an environment conducive to learning, active involvement of the learners and teachers who should have a better understanding of the system adopted. Teacher educators are seen as important in transforming Namibian education in the sense of breaking the cycle of authoritarianism and inequities that existed in schooling prior to independence in 1990, and that still continue to exist in many Namibian schools today. Much effort has been devoted to creating conditions in colleges of education, where future teachers experience the same kind of teaching and learning that is envisioned for schools in the country. To accomplish this a great deal of effort has been devoted to the professional development of teacher educators. This masters' course was initiated to address the shortcomings of teacher education in Namibia. The preparation of teacher educators to be willing and able to prepare teachers in a manner that is consistent with national educational goals has been a neglected element of educational reform in Third World countries (Taylor and Peacock, 1997). Nahas Angula defines teachers as critical agents in creating the reform in relation to several broad principals: access, equity, quality, and democracy. As Ebbutt and Elliot (1998) point out, these principles are defined in very broad terms and provide the opportunity for teachers, teacher educators, and administrators to translate the ideals in particular contexts through practical reflection and deliberation. The Namibian reforms are underpinned by democratic ideals, the intention being to develop broad participation in defining and developing the reform tenets in specific contexts. In Namibia it has been recognized that qualitative changes in classroom practice will only occur when teachers understand them and accept them as their own. It is also recognized that because many teachers have been educated under the authoritarian system of the past, conceptual changes are needed in the minds of the teachers for the reforms to be successful. These include a shift from the belief of teachers as civil servants who are merely to "deliver" a curriculum dictated from above, to one of teachers as reflective professionals who play important roles in interpreting and defining the reforms and in giving meaning to such values as learner-centered and democratic education, continuous assessment, and critical inquiry (Swarts 1998). In the case of Namibia, we have a situation where there is an attempt to fundamentally transform an authoritarian education system that emphasized repetition and rote learning of received knowledge to one where learners are active participants in the learning process and where the curriculum is relevant to and respectful of different cultural traditions and communities. The learner-centered philosophy that guides post-independence educational reforms in Namibia calls for breaking down the authoritarian teacher-student relationships of the past. It encourages teachers to begin instruction by gaining an understanding of their learners' existing knowledge, skills, and understandings and to actively involve them in the learning process toward the goal of preparing citizens for a democratic society. The background above will help the readers to understand the sections covered in this portfolio. In the first part, the portfolio looks at a case study that analyses pre- and post-independence teacher education in Namibia. This study focuses on the Rundu College of Education and investigates the implementation of the Basic Education Teacher Diploma (BETD) Broad Curriculum in relation to the History syllabus. This paper presents information on what led to post-independence teacher education reforms in Namibia and why the reform was necessary. The BETD Broad Curriculum advocates the principles underpinning teacher education reforms in Namibia. The analysis looks at whether the History syllabus applies the principles of teacher education reform in relation to practice in the classroom. This paper addresses the historical background of a History curriculum, which shows the importance of a contextual understanding of the theoretical framework. The second paper looks at the theories underpinning the pre- and post-independence curriculum and the reform process in Namibia, how each theory views knowledge and why a decision was taken to apply them. The third paper is a literature review that links the epistemological ideals of reform to the classroom situation. This paper provides the basis for a small-scale empirical research. The research proposal included in the portfolio emerged from the earlier studies and identifies a key area for investigation within the domain of my particular area of teaching. The identified research problem investigated is presented in the final paper of the portfolio. These research findings can be applied to different situations in different schools. The portfolio is completed by the presentation of a short reflection of the role of the portfolio in this masters' course.
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- Date Issued: 2005
Service provisioning in two open-source SIP implementation, cinema and vocal
- Authors: Hsieh, Ming Chih
- Date: 2013-06-18
- Subjects: Real-time data processing , Computer network protocols , Internet telephony , Digital telephone systems , Communication -- Technological innovations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4687 , http://hdl.handle.net/10962/d1008195 , Real-time data processing , Computer network protocols , Internet telephony , Digital telephone systems , Communication -- Technological innovations
- Description: The distribution of real-time multimedia streams is seen nowadays as the next step forward for the Internet. One of the most obvious uses of such streams is to support telephony over the Internet, replacing and improving traditional telephony. This thesis investigates the development and deployment of services in two Internet telephony environments, namely CINEMA (Columbia InterNet Extensible Multimedia Architecture) and VOCAL (Vovida Open Communication Application Library), both based on the Session Initiation Protocol (SIP) and open-sourced. A classification of services is proposed, which divides services into two large groups: basic and advanced services. Basic services are services such as making point-to-point calls, registering with the server and making calls via the server. Any other service is considered an advanced service. Advanced services are defined by four categories: Call Related, Interactive, Internetworking and Hybrid. New services were implemented for the Call Related, Interactive and Internetworking categories. First, features involving call blocking, call screening and missed calls were implemented in the two environments in order to investigate Call-related services. Next, a notification feature was implemented in both environments in order to investigate Interactive services. Finally, a translator between MGCP and SIP was developed to investigate an Internetworking service in the VOCAL environment. The practical implementation of the new features just described was used to answer questions about the location of the services, as well as the level of required expertise and the ease or difficulty experienced in creating services in each of the two environments. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Supervision : a Foucaultian exploration of institutional and interpersonal power relations between postgraduate supervisors, their students and the university domain
- Authors: Rau, Asta
- Date: 2005
- Subjects: Foucault, Michel, 1926-1984 -- Methodology Foucault, Michel, 1926-1984 -- Criticism and interpretation Graduate students -- Supervision of Dissertations, Academic
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1786 , http://hdl.handle.net/10962/d1003671
- Description: Supervision is widely acknowledged as influencing the quality of postgraduate theses, and by association, of postgraduates. Despite this, publications on conducting research offer far less guidance on managing the supervision relationship than on the practicalities of producing a thesis. In-depth, qualitative supervision studies are few and fewer still examine power in the supervision relationship. Michel Foucault’s insights are used to explore the question: How do postgraduate supervisors and their Master’s students experience the supervision relationship and how are the dynamics of interpersonal and institutional power implicated in these relationships? Foucault argues that power relations always involve resistances; these function primarily through institutionalized discourses to produce different forms of knowledge, one form of which is identity or subjectivity. Accordingly, power relations are explored in terms of thesis-as-product, person-as-product and the impact of both on the mediation of knowledge in the educational domain. Four institutionalized discourses in the university domain are examined: · Commercial educational management discourse: targets academics through issues of quality assurance, throughput, publication, research productivity and funding. · Anarchic educational leadership discourse: integrates quantum principles with commercial demands, change management strategies and meaningful participation. · Humanistic discourse: favours a pastoral ethic and is person-centered. · Holistic discourse: cultivates ecological sensibility and values the interconnectedness of all aspects of being-in-the-world. Data collected in sixteen semi-structured interviews with three matched supervisor-student pairs selected from the humanities and education faculties of one South African university, are presented as case studies. Data analysis combines grounded theory techniques with selected aspects of Foucault’s methods. A conceptual model is devised to analyse how participants use resistance strategies to interface their autonomy and dependency with their expectations, abilities, and professional and pastoral care. The research yields rich data in which several thematic correlations in interpersonal and institutional power dynamics are grounded. These include: the significance of supervisor-student matching; links between expectations, abilities, the way participants negotiate power and the quality of professional and pastoral care they experience; the benefit of personal affinity to thesis-as-product and person-as-product; and the impact of commercial demands on participants’ power relations. Participants tend to reproduce the discourses in which they are embedded and adopt or adapt aspects of contesting discourses to this end. Potential avenues are identified for improving supervision practice and for further research.
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- Date Issued: 2005