Isolation and characterization of genes encoding heat shock protein 70s (hsp 70s) from two species of the coelacanth, Latimeria chalumnae and Latimeria menadoensis
- Authors: Modisakeng, Keoagile William
- Date: 2007
- Subjects: Coelacanth Coelacanth -- Genetics Heat shock proteins Molecular chaperones Proteins -- Analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3971 , http://hdl.handle.net/10962/d1004030
- Description: The extant coelacanths have a close resemblance to the coelacanth fossil records dating back to 230mya. Like their predecessors, the extant coelacanths inhabit rocky caves at a depth of 100-300m below sea level. In the Comoros, the water temperature at these depths is estimated to fluctuate between 14-20°C. High-level adaptation to these environment and lack of competition are thought to have led to the morphological uniformity and slow change throughout the history of the coelacanths. Under stress conditions, proteins unfold or misfold leading to the formation of aggregates. Molecular chaperones facilitate the correct folding of other proteins so that they can attain a stable tertiary structure. In addition, molecular chaperones aid the refolding of denatured proteins and the degradation of terminally misfolded protein after cellular stress. Heat shock proteins form one of the major classes of molecular chaperones. Here we show that, despite high-level adaptation to a unique habitat and slow change, the genome of the coelacanth encodes the major and highly conserved molecular chaperone, Hsp70. Latimeria menadoensis and Latimeria chalumnae contain intronless hsp70 genes encoding Hsp70 proteins archetypal of known Hsp70s. Based on the coelacanth codon usage, we have shown that bacterial protein expression systems, particularly Escherichia coli, may not be appropriate for the overproduction of coelacanth Hsp70s and coelacanth proteins in general. Also interesting, was the discovery that like the rat Hsc70, the L. menadoensis Hsp70 could not reverse thermal sensitivity in a temperate sensitive E. coli DnaK mutant strain, BB2362. We also report the successful isolation of a 1.2 kb region of L. menadoensis hsp70 upstream regulatory region. This region contain three putative heat shock elements, a TATA- box and two CAAT-boxes. This regulatory region resembled the Xenopus, mouse, and particularly tilapia hsp70 promoters, all of which have been shown to drive the expression of reporter genes in a heat dependent manner. Taken together, this data is the first to strongly suggest an inducible Hsp70-base cytoprotection mechanism in the coelacanth. It further provides basis to formulate testable predictions about the regulation, structure and function of Hsp70s in the living fossil, Latimeria.
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- Date Issued: 2007
Evolutionary and biogeographic studies in the genus Kniphofia moench (Asphodelaceae)
- Authors: Ramdhani, Syd
- Date: 2007
- Subjects: Asphodelaceae Asphodelaceae -- Genetics Cladistic analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4220 , http://hdl.handle.net/10962/d1003789
- Description: Kniphofia, a genus of approximately 71 species, is almost entirely African with two species occurring in Madagascar and one in Yemen. Commonly known as ‘red hot pokers’ they are popular among horticulturists. The genus is also well known for its complex alpha taxonomy. To date, no studies have examined the phylogenetic relationships among species or the evolutionary history of the genus, and little work has been done on their biogeography. The main focus of this study was (i) to review the alpha taxonomy, (ii) to assess diversity and endemism in Kniphofia, (iii) to use DNA sequence data to reconstruct a specieslevel phylogeny to understand intra-generic species relationships and evolutionary processes (iv) to use phylogeographic approaches to study the biogeography and evaluate biogeographical patterns, and (v) to assess anatomical variation and determine if anatomical characters are useful for species delimitation. It was found that the genus has six centres of diversity, five of which are centres of endemism. The South African Centre is the most speciose and is also the largest centre of endemism. Kniphofia shows a strong Afromontane grassland affinity in Tropical and East Africa. In South Africa, it is found from high altitudes to coastal habitats, with the most speciose regions being Afromontane grasslands. It is thus not considered to be an Afromontane element, but rather an Afromontane associate. Five major evolutionary lineages were identified using cpDNA sequence data (trnT-L spacer), four of which are southern African. The fifth lineage is represented by material from Madagascar, East and Tropical Africa. The nuclear ITS region failed to provide resolution, as many sequences were identical. The five lineages recovered using cpDNA showed some congruence with geographic origin rather than the taxonomic arrangement based on morphology. All of the species with multiple samples were non-monophyletic. This could be due to hybridisation and/or incomplete lineage sorting. The nested clade analysis, although preliminary, did not completely agree with the phylogenetic analyses. One of the three third level nested clades appears to show fragmentation between the Cape Region, KwaZulu-Natal and northern parts of southern Africa. Furthermore, another nested clades recovered suggest a range expansion and radiation from the Drakensberg into the adjacent Drakensberg-Maputoland-Pondoland transition. Morphological species of Kniphofia exhibited substantial leaf anatomical variation and anatomical characters do not cluster samples into their morphological species. The anatomical results do not fit any geographic pattern, nor do they correspond to the lineages recovered using molecular markers or the nested clades. Leaf anatomical variation does not appear to be influenced by geographical or environmental factors. However, hybridisation may play a role but was not tested in this study. In light of the above findings it is proposed that the evolutionary and biogeographic history of Kniphofia is strongly linked to tectonic events, and Quaternary climatic cycles and vegetation changes. Tectonic events (viz. uplifts) may have resulted in vicariance events that may account for the five cpDNA lineages recovered in phylogenetic analyses, while Quaternary climatic cycles and vegetation changes may have had a more recent impact on evolution and biogeography. It is hypothesised that the ancestral area for Kniphofia was much more widespread when Afromontane grasslands were more extensive during cooler and drier glacial episodes. Kniphofia on the high mountains of Tropical and East Africa would have tracked Afromontane grasslands as they expanded their ranges in cooler periods. While during wetter and warmer interglacial periods Kniphofia would have retreated into refugia on the mountains of Tropical and East Africa, with no gene flow possible between these refugia. In South Africa, where latitude compensates for altitude, Kniphofia may have maintained a distribution that extended into the lowlands even during interglacials. A cyclic climate change hypothesis implies that populations of Kniphofia (at different phases of the climatic cycle) would have experienced periods of contractions and fragmentation followed by periods of range expansion and coalescence or secondary contact. Altitudinal shifting is proposed to be the most likely mechanism for fragmentation and range expansion, and would would possibly promoted hybridisation. Within the five lineages there is evidence for recent differentiation as the branch lengths are short, there are numerous nonmonophyletic species and numerous identical haplotypes (cpDNA and ITS) which collectively indicate a recent radiation in southern Africa. A recent radiation would also account for the taxonomic confusion and difficulty in differentiating morpho-species. These climatic events may also account for the substantial anatomical variation in southern African Kniphofia species.
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- Date Issued: 2007
Causes of persistent rural poverty in Thika district of Kenya, c.1953-2000
- Authors: Kinyanjui, Felistus Kinuna
- Date: 2007
- Subjects: Poverty -- Kenya -- History Rural poor -- Kenya -- History Agriculture -- Kenya -- History Kenya -- History Kenya -- Politics and government
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2547 , http://hdl.handle.net/10962/d1002399
- Description: This study investigates the causes of poverty among the residents of Thika District in Kenya over the period 1953-2000. Using the articulation of modes of production perspective, the study traces the dynamics of poverty to the geography, history and politics of Thika District. The thrust of the argument is that livelihoods in the district changed during the period under investigation, but not necessarily for the better. Landlessness, collapse of the coffee industry, intergenerational poverty, and the ravages of diseases (particularly of HIV/AIDS) are analysed. This leads to the conclusion that causes of poverty in Thika District during the period under examination were complex as one form of deprivation led to another. The study established that poverty in Thika District during the period under review was a product of a process of exclusion from the centre of political power and appropriation. While race was the basis for allocation of public resources in colonial Kenya, ethnicity has dominated the independence period. Consequently, one would have expected the residents of Thika District, the home of Kenya’s first president, Mzee Jomo Kenyatta, to have benefited inordinately from public resources during his rule. Kenyatta’s administration, however, mainly benefited the Kikuyu elite. The study therefore demonstrates that during the period under examination, the Kikuyu, like any other Kenyan community, were a heterogeneous group whose differences were accentuated by class relations. Subaltern groups in Thika District therefore benefited minimally from state patronage, just like similar groups elsewhere in rural Kenya. By the late 1970s, the level of deprivation in rural Kenya had been contained as a result of favourable prices for the country’s agricultural exports. But in the subsequent period, poverty increased under the pressures of world economic recession and slowdowns in trade. The situation was worse for Kikuyu peasants as the Second Republic of President Daniel Moi deliberately attempted undermine the Kikuyu economically. For the majority of Thika residents, this translated into further marginalisation as the Moi regime lumped them together with the Kikuyu elite who had benefitted inordinately from public resources during the Kenyatta era. This study demonstrates that no single factor can explain the prevalence of poverty in Thika District during the period under consideration. However, the poor in the district devised survival mechanisms that could be replicated elsewhere. Indeed, the dynamics of poverty in Thika District represent a microcosm not just for the broader Kenyan situation but also of rural livelihoods elsewhere in the world. The study recommends land reform and horticulture as possible ways of reducing poverty among rural communities. Further, for a successful global war on poverty there is an urgent need to have the West go beyond rhetoric and deliver on its promises to make poverty history.
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- Date Issued: 2007
Study of titanium, tantalum and chromium catalysts for use in industrial transformations
- Authors: Tau, Prudence Lerato
- Date: 2007
- Subjects: Titanium Tantalum Phthalocyanines Electrochemistry Photochemotherapy Chromium Spectrum analysis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4363 , http://hdl.handle.net/10962/d1005028
- Description: PART A The syntheses, spectroscopic and electrochemical characterisation of a series of titanium and tantalum phthalocyanine complexes are reported. The complexes are unsubstituted and substituted at either the peripheral or non-peripheral positions with sulphonates, aryloxy, arylthio or amino groups. The complexes mostly exhibit Qbands in the near-infrared region as well as interesting properties in different solvents. The interaction of differently sulphonated titanium and tantalum phthalocyanine complexes with methyl viologen (MV[superscript 2+]), and hence the stoichiometry and association constants are evaluated. Detailed photophysicochemical properties of the complexes were investigated and are for the first time presented with fluorescence lifetimes easily obtained from fluorescence quenching studies. The transformation of 1-hexene photocatalysed by aryloxy- and arylthio-appended complexes is also presented for the first time. The electrochemical properties of the complexes are unknown and are thus presented. Cyclic (CV) and square wave (SWV) voltammetries, chronocoulometry and spectroelectrochemistry are employed in the study of the complexes. Two oneelectron reductions and a simultaneous 4-electron reduction are observed for the unsubstituted Cl[subscript 3]TaPc. Reduction occurs first at the metal followed by ring-based processes. The tetra- and octa-substituted derivatives however exhibit peculiar electrochemical behaviour where a multi-electron transfer process occurs for complexes bearing certain substituents. For all complexes, the first two reductions are metal-based followed by ring-based processes. A comparative study of the electrocatalytic activities of the complexes towards the oxidation of nitrite is also investigated. The complexes are immobilised onto a glassy carbon electrode either by drop-dry or electropolymerisation methods. All the modified electrodes exhibit improved electrocatalytic oxidation of nitrite than the unmodified electrodes by a twoelectron mechanism producing nitrate ions. Catalytic currents are enhanced and nitrite overpotential reduced to ~ 0.60 V. Kinetic parameters are determined for all complexes and a mechanism is proposed. PART B: The syntheses and electrochemical characterisation of chromium and titanium complexes for the selective trimerisation of ethylene to 1-hexene are presented. The synthesis of the chromium complex requires simple steps while tedious steps are used for the air-sensitive titanium complex. The spectroscopic interaction of the chromium complex with the co-catalyst methylaluminoxane is investigated. The complexes are characterised by electrochemical methods such as cyclic voltammetry and spectroelectrochemistry.
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- Date Issued: 2007
Camphor-derived chiral auxiliaries: a synthetic, mechanistic and computational study
- Authors: Duggan, Andrew Robert
- Date: 2007
- Subjects: Camphor Chirality Asymmetric synthesis
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4415 , http://hdl.handle.net/10962/d1006772
- Description: A broadly based approach has been undertaken to the development and use of camphor derivatives as chiral auxiliaries in asymmetric synthesis – an approach which has embraced synthetic, mechanistic and computational studies. The unambiguous characterization of mono- and dihydroxy-derivatives, obtained by reduction of chiral camphor ether dimers, has been achieved through detailed one- and two-dimensional NMR spectroscopic analysis. The resulting data has been used to establish both the regio- and stereochemistry of the hydroxyl groups. A camphor-derived cyclic iminolactone has been shown to provide a convenient platform for the synthesis of chiral α-amino acids, stereoselective monoalkylation of the iminolactone affording a range of products in yields of 52 - 65 % with up to 85 % d.e. The attempted development of chiral bifunctional Morita-Baylis-Hillman substrates has revealed an unexpected equilibration between isomeric bornane 2,3-diol monoacrylates via acid-catalysed intramolecular transesterification. A detailed [superscript 1]H NMR-based kinetic study of the rearrangement in various media and at various temperatures has permitted the determination of the kinetic and thermodynamic parameters. A computational study at the DFT level has been used to explore the potential energy surfaces of the acid-catalysed and uncatalysed transesterification of the monoacrylate esters. The theoretical data supports the involvement of cyclic intermediates and has provided a rational basis for predicting the favoured reaction pathways. Novel camphor-derived phenyl sulfonate esters and N-adamantylsulfonamides have been synthesised for use as chiral auxiliaries in the Morita-Baylis-Hillman reaction. Modeling at the Molecular Mechanics level has provided useful insights into possible conformational constraints and an adamantyl sulfonate auxiliary has been successfully used in the stereoselective synthesis of a range of products, generally in excellent yield and with up to 95 % d.e.
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- Date Issued: 2007
A transdisciplinary explanatory critique of environmental education
- Authors: Price, Leigh
- Date: 2007
- Subjects: Environmental education Business enterprises -- Environmental aspects Corporations -- Environmental aspects Social responsibility of business Social responsibility of business -- Study and teaching Environmental education -- Philosophy Environmental ethics -- Study and teaching
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1804 , http://hdl.handle.net/10962/d1003689
- Description: This study originates out of my experience as an environmental educator working within business and industry in Zimbabwe and South Africa. It is motivated by my observation that, despite much environmental rhetoric and training, environmental education in industry rarely leads to significant advances towards environmental protection. I assume that the problem of the mismatch between rhetoric and action involves both semiotic and non-semiotic components and therefore, after a thorough exploration of my methodological options, I adopt a qualitative transdisciplinary textual analysis of relevant documents using Fairclough’s Critical Discourse Analysis and Bhaskar’s Dialectical Critical Realism, with some insights taken from Bhaskar’s more recent concept of Meta-Reality. My main conclusions from the study indicate that causally efficacious philosophical mistakes, relating to theories of structure/agency and theories of epistemology, are an important aspect of the problem being considered. Specifically, I demonstrate that these mistakes function to buttress ideology and its attendant contradictions which in turn function to provide the preconditions that maintain inequalities and poor environmental practice in business and industry. Prior and current events, such as climate change and the trend towards globalisation, the ‘free market economy’ and psychological characteristics of the author, relevant to the problem, are also important. In line with Bhaskar’s emancipatory aim for explanatory critique, I end with tentative recommendations for a re-imagined environmental education for business and industry which require (un)action. Consistent with my methodological choices, my recommendations have a (qualified) universal application, despite my focus on texts from South Africa and Zimbabwe. My recommendations are summarised below: • there should be consistency between theory and practice such that performance contradictions are avoided; • we should not act from a fear of survival based on past, no longer relevant experiences (e.g. from childhood) as this is unlikely to be an adequate base for present actions; • we should avoid voluntarism by acting with the resources at our disposal, based on a true understanding of our strengths and weaknesses and our own specificities; • we should avoid assuming the stance of the ‘victim’ by refusing to blame other agents or circumstances, without distorting or underestimating the causal efficacy of those agents or circumstances (related to avoiding voluntarism, whilst nevertheless not resorting to determinism either); • we should direct our action towards the abolition of inequalities and master-slave relationships (related to the avoidance of performance contradictions); • we should act from the position of epistemological humility, rather than from the position of epistemological privilege; • we should consider action as ‘shedding’ based on an understanding of the Transformational Model of Social Activity (TMSA); and • we should consider learning to be ‘shedding’ based on the necessity of (un)knowledge, or ignorance, as a requirement of arriving at relatively new knowledge. This study is also a contribution to contemporary methodological discussions relevant to Critical Discourse Analysis in that it extends these discussions to include psychoanalytical (as well as the more familiar phenomenological and ideological) depth explanations of lived illusion. Furthermore, this study is an experimental attempt to apply the concept of ‘meta-reflexivity’ in Critical Discourse Analysis.
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- Date Issued: 2007
Passing the spear : a grounded theory study of the influence of family business value sets on succession planning in black family-owned businesses
- Authors: Musengi, Sandra
- Date: 2007
- Subjects: Grounded theory Family-owned business enterprises Family-owned business enterprises -- Succession Family-owned business enterprises -- Management Business enterprises, Black Business planning
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1192 , http://hdl.handle.net/10962/d1007056
- Description: Literature suggests that a small number of family businesses are able to make the transition from the founder to other family members with a common reason cited being the lack of planning. This study aims to build understanding of leadership succession in family businesses by focusing on the influence of a founder's family business value set on the succession planning process in Black family-owned businesses. Using the Strauss and Corbin (1990) grounded theory method, this study develops a theory of succession planning of Black familyowned businesses labelled Passing the Spear which is comprised of three stages based on an analysis of 21 qualitative interviews. The Spear was an analogy used to represent both the values of the founder and the family business, thus in Passing the Spear, founders where essentially performing a dual transfer of their values and leadership to the successor. The implementation of the process Passing the Spear was influenced by the family business value set of the founder, which in this study, were labelled as Traditional, Progressive, and Transitional. These value sets were distinguished by their behaviour regarding their choice of successor where founders with a traditional value set exhibited gatekeeping behaviour, while the behaviour of founders with a progressive value set was labelled navigating, and finally, founders with a transitional value set demonstrated behaviour labelled exploring. Furthermore, it was found that after the successor had been chosen, founders appeared to follow a generic succession planning process, however, the ease and timing of implementation was influenced by the family business context, evidence of being proactive and the degree of family business resilience. The process of Passing the Spear comprised of three stages of (a) Showing the spear where the founder's focus is on the induction and socialization of the successor using the strategies of bringing the successor into the family business and managing the family-business interface using relationships; (b) Explaining the spear entailed founders using the strategies of sharing knowledge and teaching the successors about the family business; and ( c) Sharing the spear was where founders focused on empowering the successors by implementing strategies of sharing responsibilities and learning from the successor.The process provides insights into the influence of family business values on the succession planning process and can be useful for founders of Black family-owned businesses in planning for succession in their businesses. In addition, the study provides another perspective of succession planning and offers a contribution to the literature for understanding succession in Black family-owned businesses.
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- Date Issued: 2007
The idea of regionalism in West and Southern Africa : a critical social enquiry
- Authors: Lindsay, Albert Domson
- Date: 2007
- Subjects: Regionalism -- Africa, West Regionalism -- Africa, Southern Africa, West -- Politics and government Africa, Southern -- Politics and government Africa -- Foreign relations
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2848 , http://hdl.handle.net/10962/d1006118
- Description: Traditional views on International Relations dominate regional analyses. These invariably emphasize the dominance of state and market forces in inter-state relations. Experiences and expectations of people are less prominent in these discourses, and the practices they foster. This thesis critically analyses the regional processes in West and southern Africa within the framework of Critical Theory. It argues that these processes are constrained by instability and the increasing legitimacy crises of the State. The thesis demonstrates that the State, through exclusive nationalist practices, hinder the growth of a cosmopolitan order, and it argues that neo-liberal regionalism is a contested phenomenon because of its exclusive nature. Finally, the thesis suggests steps needed to resolve the legitimacy crises and to build an inclusive regional order, based on cosmopolitan values.
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- Date Issued: 2007
Contributions to the use of microalgae in estuarine freshwater reserve determinations
- Authors: Snow, Gavin Charles
- Date: 2007
- Subjects: Microalgae -- South Africa , Estuarine ecology -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10620 , http://hdl.handle.net/10948/709 , Microalgae -- South Africa , Estuarine ecology -- South Africa
- Description: The ecologist Garrett Hardin (1968) introduced a useful concept called the tragedy of the commons, which describes how ecological resources become threatened or lost. The term “commons” is based on the commons of old English villages and is symbolic of a resource that is shared by a group of people. If every person were to use each resource in a sustainable fashion it would be available in perpetuity. However, if people use more than their share they would only increase their personal wealth to the detriment of others. In addition, an increase in the population would mean that the size of each share would have to decrease to accommodate the larger number of people. As a result, resources are threatened by personal greed and uncontrolled population growth. Freshwater is an example of a common resource that is under threat in South Africa where the average annual rainfall is less than 60 percent of the global average (Mukheibir & Sparks 2006). The increasing demands for freshwater as well as its eutrophication are major concerns with regards to estuarine health, environmental resource management and human health. The correct management of water is necessary to ensure that it is utilised in a sustainable manner. The National Water Act (No. 36 of 1998) has provided the rights to water for basic human needs and for sustainable ecological function; the Basic Human Needs Reserve and Ecological Reserve are both provided as a right in law. The amount of water necessary for an estuary to retain an acceptable ecological status, known as the Estuarine Ecological Reserve, is determined through the implementation of procedures (rapid, intermediate or comprehensive) compiled by the Department of Water Affairs and Forestry (1999) in its Resource Directed Measures (RDM) for the Protection of Water Resources. The impact of restricted flow on estuaries can be reduced by manipulating the water released from impoundments, the regulation of water abstractions within the river catchment or both (Hirji et al. 2002). The reserve assessment method is designed to evaluate ecosystem requirements by employing groups of specialists from different disciplines. In South Africa, this includes hydrologists, sedimentologists, water chemists and biologists (including microalgae specialists). The use of microalgae in ecological assessments has largely been based on research that was initiated at the Nelson Mandela Metropolitan University (formerly University of Port Elizabeth) and subsequently at Rhodes University (Grahamstown) and the University of KwaZulu Natal (Durban). The microalgal research can be divided into two main focus areas; phytoplankton and benthic microalgae
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- Date Issued: 2007
Development of an in-situ ß-D-Glucuronidase diagnostic moraxella-based biosensor for potential application in the monitoring of water polluted by faecal material in South Africa
- Authors: Togo, Chamunorwa Aloius
- Date: 2007
- Subjects: Water quality management -- South Africa Water quality bioassay -- South Africa Sewage sludge -- South Africa -- Management Water -- Purification -- Biological treatment -- South Africa Biosensors
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3947 , http://hdl.handle.net/10962/d1004006
- Description: The prevention of outbreaks of waterborne diseases remains a major challenge to public health service providers globally. One of the major obstacles in this effort is the unavailability of on-line and real-time methods for rapid monitoring of faecal pollution to facilitate early warning of contamination of drinking water. The main objective of this study was to develop a β-glucuronidase (GUD)-based method that could be used for the on-line and real-time monitoring of microbial water quality. GUD is a marker enzyme for the faecal indicator bacteria Escherichia coli. This enzyme breaks down the synthetic substrate p-nitrophenyl-β-D-glucuronide (PNPG) to D-glucuronic acid and p-nitrophenol (PNP), which turns yellow under alkaline pH. The enzymatically produced PNP was used to detect GUD activity. In situ GUD assays were performed using running and stagnant water samples from the Bloukrans River, Grahamstown, South Africa. The physico-chemical properties of environmental GUD were determined, after which a liquid bioprobe and a microbial biosensor modified with Moraxella 1A species for the detection of the enzyme activity were developed. In order to determine the reliability and sensitivity of these methods, regression analyses for each method versus E. coli colony forming units (CFU) were performed. The storage stabilities of the bioprobe and biosensor were also investigated. The physico-chemical properties of in situ GUD were different from those of its commercially available counterpart. The temperature optimum for the former was between 35 and 40 °C while for the latter it was 45 °C. Commercial (reference) GUD had a pH optimum of 8.0 while the environmental counterpart exhibited a broad pH optimum of between pH 5.0 and 8.0. The liquid bioprobe had a limit of detection (LOD) of GUD activity equivalent to 2 CFU/100 ml and a detection time of 24 h. The method was less labour intensive and costly than the culturing method. The liquid bioprobe was stable for at least four weeks at room temperature (20 ± 2 °C). The biosensor was prepared by modifying a glassy carbon electrode with PNP degrading Moraxella 1A cells. The biosensor was 100 times more sensitive and rapid (5-20 min) than the spectrophotometric method (24 h), and was also able to detect GUD activity of viable but non-culturable cells. Thus it was more sensitive than the culturing method. Furthermore, the biosensor was selective and costeffective. The possibility of using a Pseudomonas putida JS444 biosensor was also investigated, but it was not as sensitive and selective as the Moraxella 1A biosensor. The Moraxella biosensor, therefore, offered the best option for on-line and real-time microbial water quality monitoring in South African river waters and drinking water supplies.
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- Date Issued: 2007
A phenomenological study of women primary school heads' experiences as educational leaders in post colonial Zimbabwe
- Authors: Muzvidziwa, Irene
- Date: 2013-06-26
- Subjects: Educational leadership -- Zimbabwe Women school administrators -- Zimbabwe School management and organization -- Zimbabwe Women in education -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1949 , http://hdl.handle.net/10962/d1008200
- Description: This research study was carried out in order to gain an understanding of the experiences of women primary school heads, their perceptions of their roles as leaders, the challenges they face and how they dealt with them. The study focused on the lived experiences of five women in Zimbabwe's primary schools. Literature relating to the issues and experiences of women in educational leadership within school contexts and the conceptual framework is examined. The importance of leadership has been emphasised in the literature of school effectiveness. Leadership theories tended to emphasise measurability and effectiveness of leadership, oversimplifying the complexity of leadership phenomenon. These features reflect research approach adopted by researchers from a positivist orientation. This study is an in-depth qualitative study conducted along the lines suggested by a phenomenological-interpretivist design with emphasis on rich contextual detail, close attention to individual's lived experience and the bracketing of pre-conceived notions of the phenomenon. Views and experiences based on the participants' perspectives are described through in-depth interviews which were dialogical in nature. Through this approach, I managed to grasp the essences of the lived experiences of women The research highlights the women's perceptions of themselves as educational leaders. What emerges is the variety of approaches to handling challenges. My findings show a rich and diverse culture of creativity in the way participants adopted a problem-solving strategy, which is not reflected in the mainstream leadership. Though educational leadership emerges as a complex phenomenon, with alternative approaches to educational research, there is high potential for increased understanding of woman's leadership, its importance and implications for school. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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The biology and systematics of South African pipefishes of the genus Syngnathus
- Authors: Mwale, Monica
- Date: 2007
- Subjects: Pipefishes -- South Africa Syngnathus -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5237 , http://hdl.handle.net/10962/d1005080
- Description: Syngnathus the most speciose genus in the family Syngnathidae is widely in the Atlantic and Eastern Pacific oceans. However, it is poorly represented in the Indian Ocean with the only two species, Syngnathus temminckii and S. watermeyeri occurring in Southern African estuaries and coastal areas. Syngnathus temminckii the most common South African pipefish has been synonymised with S. acus, as the morphological and genetic divergence between these two populations has not been documented. There is also uncertainty in the taxonomic status of S. watermeyeri, an endemic estuarine pipefish that is restricted to two Eastern Cape estuaries. The purpose of this study was therefore to compare biological, morphological and genetic variation of South African Syngnathus species among different populations/locations, and with European populations of S. acus. Sixteen meristic and ten morphometric characteristics were quantified from specimens obtained from field as well as various international natural history museum collections. Univariate (ANOVA) and multivariate (principal component analysis and discriminant analyses) analyses were used to assess morphological differences among the species. Morphometric variables were adjusted as ratios of the standard length and using an allometric procedure. ANCOVA analysis indicated significant differences between S. acus and S. temminckii for the relationships of the standard length (SL) and all morphological characters. There was no significant correlation between SL and snout length, snout depth, inter-orbital width and trunk depth for S. watermeyeri. The analyses provided evidence for distinct populations of S. acus, S. temminckii and S. watermeyeri although morphological character differentiation was greater between S. watermeyeri and the other two larger species. Although, significant differences were observed for meristic characters, pairwise comparisons did not reflect a clear pattern of variability. Most of the measured morphological characters contributed more than 70% to the morphological variation between the populations. Plot of the canonical scores for the variables resulted in the specimens clustering according to species groups and locations of S. temminckii. Sequences of 750 base pairs of the mitochondrial cytochrome gene from 11 localities were compared with published sequences of other species of Syngnathus. Phylogenetic analysis was performed using parsimony, maximum likelihood (ML) and Bayesian inference (BI). The South African species were revealed to be sister-taxa with about 6 % divergence, while S. temminckii and S. acus had about 11% sequence divergence. 20 haplotypes among 46 total specimens from the three species. Gene flow was estimated at approximately 3 migrants per generation between the two South African populations and about 1 per generation between S. temminckii and S. acus. Such strong stock structuring among presumably recently established post-Pliocene (< 2 Million years ago) populations suggests that these species are reproductively isolated. Morphological and genetic variation observed in this study combined with current knowledge of life history attributes of the South African pipefishes indicate that conservative management decisions are necessary until the patterns and extent of differentiation among populations species-wide can be investigated further. It is thus being proposed that the name of the South African population of S. acus be changed to Syngnathus temminckii (Kaup, 1856).
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- Date Issued: 2007
Masculinity, citizenship and political objection to compulsory military service in the South African Defence Force, 1978-1990
- Authors: Conway, Daniel John
- Date: 2013-08-15
- Subjects: Conscientious objectors -- South Africa End Conscription Campaign (South Africa) National service -- South Africa Draft -- South Africa South Africa -- Military policy Masculinity -- South Africa South Africa. South African Defense Force Gays in the military -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2601 , http://hdl.handle.net/10962/d1008383
- Description: This thesis conceptualises compulsory military service and objection to it as public performative acts that generate gendered and political identity. Conscription was the primary performance of citizenship and masculinity for white men in apartheid South Africa. Conscription was also a key governance strategy both in terms of upholding the authority of the state and in engendering discipline in the white population. Objection to military service was therefore a destabilising and transgressive public act. Competing conceptualisations of masculinity and citizenship are inherent in pro and anti-conscription discourses. The refusal to undertake military service places men outside the accepted means of graduating to ' real' manhood and patriotic citizenship. Although objection can be an iconic and transgressive act, objectors have an essentially ambivalent subjectivity in the public realm. Objectors are 'strangers' in a socially constructed and gendered binary of 'insiders' and 'outsiders' . This ambivalent status creates opportunities but also constraints for the performance of objection. The thesis analyses the effectiveness of objectors' performances and argues that there is a distinction between a radical challenge to hegemonic conceptions of militarised masculinity and citizenship and assimilatory challenges. The tension between radicalism and assimilation comes to the fore in response to the state's attacks on objectors. The militarised apartheid state is defined as not only masculine but heteronormative terms and it is the deployment of sexuality that is its most effective means of stigmatising and restricting the performance of objection. The thesis uses interview material, archival data and case studies and concludes that objectors (and their supporters) weaved multiple narratives into their performances but that as the 1980s progressed, the performance of objection to conscription became assimilatory and this demonstrates the heteronormativity of the state, military service and the public realm. , KMBT_363 , Adobe Acrobat 9.54 Paper Capture Plug-in
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Opportunity and constraint : historicity, hybridity and notions of cultural identity among farm workers in the Sundays River Valley
- Authors: Connor, Teresa Kathleen
- Date: 2007
- Subjects: Agricultural laborers -- South Africa -- Relocation Addo Elephant National Park (South Africa) Xhosa (African people) -- South Africa -- Eastern Cape -- Ethnic identity Forced migration -- South Africa -- Eastern Cape Land settlement -- South Africa -- Government policy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2117 , http://hdl.handle.net/10962/d1008367
- Description: This thesis focuses on relationships of opportunity and constraint among farm workers in the Sundays River Valley (SRV), Eastern Cape Province. Relationships of 'constraint' include those experiences of displacement and forced removal and war, including forced removals by the apartheid state in 1960 and 1970. Relationships of 'opportunity' include the ways in which residents in the SRV have contested their experiences of upheaval and domination, and the formation of a regional sense of place and belonging/ investigate how farm workers actually draw elements of locality and identity from their experiences of upheaval, and how displacement bolsters feelings of belonging and place. Instead of viewing displacement as a once-off experience, this thesis investigates displacement in historical terms, as a long-term, 'serial' experience of human movement, which is continued in the present- specifically through the creation of the Greater Addo Elephant National Park. I concentrate on developing a spatialised and cultural notion of movemenUplacement. 'Place' is investigated as a term that refers to rather indeterminate feelings of nostalgia, memory and identity, which depend on a particular connection to territory (ie: 'space'). I emphasise that elements of place in the SRV are drawn from and expressed along dualistic lines, which juxtapose situations of opportunity and constraint. In this way, farm workers' sense of connection to farms and ancestral territory in the SRV depends on their experiences of stable residency and work on farms, as well as their memories of removal from land in the area. I emphasise that those elements of conservatism (expressed as 'tradition' and Redness) among Xhosa-speaking farm workers are indications of a certain hybridity of identity in the region, which depend on differentiation from other groups (such as so-called 'coloured' farm workers and 'white' farmers), as well as associations between these groups. This thesis lays emphasis upon those less visible and definable 'identities' in the Eastern Cape Province, specifically by shifting focus away from the exhomeland states of the Ciskei and Transkei, to more marginal expressions of identity and change (among farm workers) in the Province. I point out that labourers cannot solely be defined by their positions as farm workers, but by their place and sense of cultural belonging in the area. In this sense, I use the idea of work as a loaded concept that can comment on a range of cultural attitudes towards belonging and place, and which is firmly embedded in the private lives of labourers - beyond their simple socio-economic conditions of farm work. I use Bourdieu's conception of habitus and doxa to define work as a set of dispositions that have been historicised and internalised by workers to such an extent, that relationships of domination are sometimes inadvertently obscured through their apparent 'naturalness'. Moreover, I point out that work can be related to ritualised action in the SRV through the use of performance and practice-based anthropological theory. Both work and ritual are symbolic actions, and are sites of struggle within which workers express themselves dualistically. Rituals, specifically, are dramatic events that combine disharmonious and harmonious social processes - juxtaposing the powerlessness of workers (on farms), and the deep sense of belonging and place in the SRV. I argue that the deep historical connections in the SRV have largely been ignored by conservationists in the drive to establish new protected zones (such as the Greater Addo Elephant National Park), and that a new model of shared conservation management is needed for this Park.
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- Date Issued: 2007
Towards knowing through doing : improving the societal relevance of systematic conservation assessments
- Authors: Knight, Andrew Thomas
- Date: 2007
- Subjects: Conservation of natural resources -- Planning , Nature conservation -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10598 , http://hdl.handle.net/10948/711 , Conservation of natural resources -- Planning , Nature conservation -- South Africa
- Description: Systematic conservation assessments are spatially-explicit techniques for prioritising areas for the implementation of conservation action. There has been considerable reference in the peer-reviewed literature as to the usefulness of these tools, which appear to be primarily used by academics for theoretical research. A literature review and author survey reveals the peer-reviewed literature is largely theoretical, although conservation action results more frequently than reported. The effectiveness of these interventions is generally described as only ‘fairly effective’. This general trend, coupled with previous personal failures in translating systematic conservation assessments into effective conservation action triggered an explicit process of social learning implemented as action research. It examined the workings of the Subtropical Thicket Ecosystem Planning (STEP) project, which included development of a systematic conservation assessment. Systematic conservations assessments simply provide information on where action should be implemented, and so are only useful if situated within broader operational models for conservation planning. Most operational models presented in the peer-reviewed literature are primarily focused upon the testing ecological data, not upon the delivery of conservation action. A new operational model for conservation planning is presented which more accurately reflects the ‘real-world’ process of conservation planning. An implementation strategy is an essential complement to a systematic conservation assessment. It describes how specific, explicitly-stated goals will be achieved, who is accountable for undertaking these activities, and the resources required. As the Implementation Specialist for the STEP Project, I co-lead the collaborative development of an implementation strategy with stakeholders that aimed to mobilise resources towards achieving common goals. Whilst the development and initial uptake of the strategy was good, subsequent implementation has flounder. The reasons for this are explored. The ultimate pragmatic goal of a conservation planning process is the establishment of effective social learning institutions. These develop common visions, mobilise collective action, and adaptively learn and refine their conservation activities. Thicket Forum is one xi such institution established through the STEP Project. My involvement with Thicket Forum since 2004 in implementing an adaptive learning approach facilitates collaboration between land managers, government and research organisations. Systematic conservation assessments evolved in response to the ad hoc way in which protected areas were implemented, leaving unrepresentative, biased protected area networks. Most research is theoretical and without an intimate understanding of the social-ecological system of a planning region, notably opportunities and constraints for implementing conservation action. Highlighting the importance of an approach which is flexible, not only in space, but in time, which can capitalise upon implementation opportunities, is important for stemming the myth that opportunism is the nemesis of systematic conservation assessments. To this end, conservation planners have been slow to include factors influencing effective implementation in systematic conservation assessments. Many studies which identify candidate protected area networks, first, fail to identify the specific instrument(s) to be applied, and second, assume all intact land is available. Having mapped the willingness of land managers in the Albany District, South Africa, to sell their land, it is demonstrated the majority of targets fail to be achieved because land managers will not sell. Knowing this, the current focus of gathering ever-more ecological data is misplaced. Human, social and economic factors influence target achievement, efficiency and spatial configuration of priority areas. Selecting important areas for conservation, particularly at the local-scale, requires the mapping of factors which define opportunities for conservation. Land manager willingness to collaborate and participate, entrepreneurial orientation, conservation knowledge, social capital, and local champions were applied using a method of hierarchical clustering to identify land managers who represent conservation opportunities for private land conservation initiatives.
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- Date Issued: 2007
Pharmaceutical analysis and quality of complementary medicines : sceletium and associated products
- Authors: Patnala, Satya Siva Rama Ranganath Srinivas
- Date: 2007
- Subjects: Alternative medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3872 , http://hdl.handle.net/10962/d1018263
- Description: There has been an upsurge in the use of Complementary and Alternate Medicines (CAMs) in both developed and developing countries. Although herbal medicines have been in use for many centuries, their quality, safety and efficacy are still of major concern. Many countries are in the process of integrating CAMs into conventional health care systems based on the knowledge and use of traditional medicines. The quality control (QC) of herbal products usually presents a formidable analytical challenge in view of the complexity of the constituents in plant material and the commercial non-availability of appropriate qualified reference standards. Sceletium, a genus belonging to the family Aizoaceae, has been reported to contain psychoactive alkaloids, specifically mesembrine, mesembrenone, mesembrenol and some other related alkaloids. Sceletium is marketed as dried plant powder and as phyto-pharmaceutical dosage forms. Sceletium products and plant material marketed through health shops and on the internet are associated with unjustified claims of specific therapeutic efficacy and may be of dubious quality. Validated analytical methods to estimate Sceletium alkaloids have not previously been reported in the scientific literature and the available methods have focused only on qualitative estimation. Furthermore, since appropriate markers were not commercially available for use as reference standards, a primary objective of this study was to isolate relevant compounds, qualify them as reference standards which could be applied to develop appropriate validated qualitative and quantitative analytical methods for fingerprinting and assay of Sceletium plant material and dosage forms. The alkaloidal markers mesembrine, mesembrenone and ∆⁷ mesembrenone were isolated by solvent extraction and chromatography from dried plant material. Mesembranol and epimesembranol were synthesised by hydrogenation of the isolated mesembrine using the catalyst platinum (IV) oxide and then further purified by semi-preparative column chromatography. All compounds were subjected to analysis by ¹H, ¹³C, 2-D nuclear magnetic resonance and liquid chromatography-tandem mass spectroscopy. Mesembrine was converted to hydrochloride crystals and mesembranol was isolated as crystals from the hydrogenation reaction mass. These compounds were analysed and characterised by X-ray crystallography. A relatively simple HPLC method for the separation and quantitative analysis of five relevant alkaloidal components in Sceletium was developed and validated. The method was applied to determine the alkaloids in plant material and dosage forms containing Sceletium. An LCMS method developed during the study provided accurate identification of the five relevant Sceletium alkaloids. The method was applied for the quantitative analysis and QC of Sceletium plant material and its dosage forms. This LCMS method was found to efficiently ionize the relevant alkaloidal markers in order to facilitate their detection, identification and quantification in Sceletium plant material as well as for the assay and QC of dosage forms containing Sceletium. The chemotaxonomy of some Sceletium species and commercially available Sceletium dosage forms were successfully studied by the LCMS method. The HPLC and LCMS methods were also used to monitor the bio-conversion of some of the alkaloids while processing the plant material as per traditional method of fermentation. Additionally a high resolution CZE method was developed for the separation of several Sceletium alkaloids in relatively short analysis times. This analytical method was used successfully to fingerprint the alkaloids and quantify mesembrine in Sceletium and its products. Sceletium species grown under varying conditions at different locations, when analyzed, showed major differences in their composition of alkaloids and an enormous difference was found to exist between the various species with respect to the presence and content of alkaloids. Sceletium and its products marketed through health shops and the internet may thus have problems with respect to the quality and related therapeutic efficacy. The QC of Sceletium presents a formidable challenge as Sceletium plants and products contain a complex mixture of compounds. The work presented herein contributes to a growing body of scientific knowledge to improve the QC standards of herbal medicines and also to provide vital information regarding the selection of plant species and information on the specific alkaloidal constituents to the cultivators of Sceletium and the manufacturers of its products.
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- Date Issued: 2007
A critical ethnographic study of report writing as a literacy practice by automotive engineers
- Authors: Harran, Marcelle
- Date: 2007
- Subjects: English language -- Written English -- South Africa Written communication -- South Africa Literacy -- Social aspects -- South Africa Engineers -- Language -- South Africa Communication in engineering -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1476 , http://hdl.handle.net/10962/d1003357
- Description: This study describes the social practices involved in the situated activity of report writing in an engineering automotive discourse community in South Africa. In particular, the study focuses on the subjectivity of predominantly English Second Language (ESL) engineers writing reports by determining what literacy means to them and what meanings they give to dominant literacy practices in report writing, especially feedback in text production. In the South African engineering workplace, because of the diversity and complexity of language and identity issues, the appropriation of the required literacy skills tends to be multifaceted. This context is made more complex as English is the business language upon which engineering is based with engineering competence often related to English proficiency. Therefore, the study is located within the understanding that literacy is always situated within specific discoursal practices whose ideologies, beliefs, power relations, values and identities are manifested rhetorically. The basis for this critical theory of literacy is the assertion that literacy is a social practice which involves not only observable units of behaviour but values, attitudes, feelings and social relationships. As the institution’s socio-cultural context in the form of embedded historical and institutional forces impact on writer identity and writing practices or ways of doing report writing, notions of writing as a transparent and autonomous system are also challenged. As critical ethnography is concerned with multiple perspectives, it was selected as the preferred methodology and critical realism to derive definitions of truth and validity. Critical ethnography explores cultural orientations of local practice contexts and incorporates multiple understandings providing a holistic understanding of the complexity of writing practices. As human experience can only be known under particular descriptions, usually in terms of available discourses such as language, writing and rhetoric, the dominant practices emerging in response to the report acceptance event are explored, especially that of supervisor feedback practices as they causally impact on report-writing practices during the practice of report acceptance. Although critical realism does not necessarily demonstrate successful causal explanations, it does look for substantial relations within wider contexts to illuminate part-whole relationships. Therefore, an attempt is made to find representativeness or fit with situated engineering literacy practices and wider and changing literacy contexts, especially the impact of Higher Education and world Englishes as well as the expanding influence of technological and digital systems on report-writing practices.
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- Date Issued: 2007
Field and laboratory analyses of manual tasks in the South African automotive industry
- Authors: James, Jonathan Peter
- Date: 2007
- Subjects: Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5121 , http://hdl.handle.net/10962/d1005199 , Automobile industry and trade -- South Africa -- Safety measures , Human engineering -- South Africa , Automobile industry workers -- South Africa -- Health risk assessment , Industrial safety -- South Africa
- Description: The present study adopted a “field-laboratory-field” approach in the assessment of the efficacy of ergonomics interventions specific to two selected tasks evaluated in a South African automotive industry. Initial field testing was conducted in an Eastern Cape (South Africa) automotive plant where high risk areas were identified during walkthrough ergonomics surveys in conjunction with interaction with operators. Temporal factors and working postures of 12 industrial workers were recorded and observed, while physiological and perceptual responses were assessed. Two priority areas were focused upon for analysis, namely the Paintshop and Bodyshop with the former identified as being the more taxing of the two tasks. Responses of 30 students participating in rigourously controlled laboratory simulations were subsequently collected while completing the two tasks, namely the Paintshop Trolley Transfer (PTT) and Car Door Carriage (CDC) for participants. Working postures, kinematic, physiological and perceptual responses were assessed pre- and post-intervention. Following the laboratory experimentation a basic re-evaluation was conducted at the plant to assess whether the proposed changes had a positive effect on working postures, physiological and perceptual responses. The results of the preliminary field investigation revealed a prevalence of awkward working postures and excessive manual work in both areas. Laboratory experimentation revealed a notable reduction in task demands pre- versus post-intervention. The PTT mean lean angle for two-handed pre-intervention pulling observations of 23.7° (±3.51) was reduced to 13.9° (±2.21) post-intervention. Low back disorder (LBD) risk was reduced during the two-handed pull intervention (from 36.8% ±8.03 to 21.7% ±5.31). A significant decrement in heart rate responses from 103 bt.min-1 (±11.62) to 93 bt.min[superscript -1] (±11.77) was recorded during the two-handed symmetrical pushing intervention. The electromyography (EMG) responses for one-handed pushing and pulling pre-intervention showed the highest levels of muscular activity in the right medial deltoid due to an awkward and asymmetrical posture. CDC responses demonstrated that minor changes in the storage height of the door resulted in a significant reduction in sagittal flexion from 28.0° (±4.78) to 20.7° (±5.65). Predictions of average probability of LBD risk were significantly reduced from 50.3% (±5.91) to 39.8% (±5.10) for post-intervention car door lifting. In addition, the greatest reduction in EMG activity as a %MVC was achieved during sub-task ii (reduced from 35.1 to 13.7% and 30.5 to 13.9% for left and right erector spinae respectively) which was associated with the introduction of the transfer trolley for the door transfer phase of the CDC. Re-evaluation in the automotive plant revealed that the most notable change has been the implementation of automated ride on trolleys in the Paintshop. The Bodyshop area has also been modified to allow more effective job rotation and the step into the storage bin has been reduced via a “low-cost” stepping platform. Mean heart rate recordings were reduced from 94 (±9.77) bt.min[superscript -1] to 81 (±3.72) bt.min[superscript -1] in the Paintshop. Overall the results demonstrate the effect of “low-cost” interventions in reducing the physical stresses placed on workers in the automotive industry where much of the work is still done manually.
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- Date Issued: 2007
On the microstructure and physical properties of hot pressed (Hf, Ti) C
- Authors: Heiligers, Christiané
- Date: 2007
- Subjects: Transition metal carbides
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10532 , http://hdl.handle.net/10948/523 , http://hdl.handle.net/10948/d1011722 , Transition metal carbides
- Description: The microstructure and physical properties of hot pressed (Hf, Ti) C have been investigated with the aim of producing a cutting tool material with similar hardness to that of WC-Co and TiC-based cermets. Sintered samples were hot pressed from HfC0.7 and TiC0.9 powders using powder metallurgical techniques and the processing cycle was optimized for this system. Ni was used as a binder in selected samples and C black was added to compensate for sub-stoichiometry and to aid in the reduction of oxides formed during milling. Microstructural analyses were performed by scanning and transmission electron microscopy (SEM and TEM) and the composition was determined from X-ray diffraction (XRD) and energy dispersive X-ray spectrometry (EDS). The physical properties measured are density and Vickers hardness, and the indentation fracture toughness was determined using the Shetty formula. The fundamental interactions between HfC, TiC and Ni during hot pressing were investigated and the results obtained used to explain the microstructure that develops in samples made from powder mixtures. The interactions studied are the inter-diffusion of HfC and TiC through the solid state, and the dissolution and re-precipitation rate of the carbides in a liquid Ni binder. EDS analysis revealed that the rate at which Ti diffuses into HfC is higher than the rate at which Hf diffuses into TiC. Upper limits to the diffusion coefficients for these processes are determined and show that solid solution carbides will form from HfC + TiC powder mixtures at 2000 ºC in 1 hour if the average powder particle size is less than 5 μm. The diffusion rates decrease with a decrease in hot pressing temperature but mass transport between the phases can be enhanced by addition of a metallic binder. TEM and EDS analysis shows that Ni wets TiC more efficiently than HfC and that the solubility of TiC in Ni is also higher than that of HfC. The grain size of the carbide phases increases with an increase in the rate at which they dissolve into and re-precipitate from the liquid binder. The crystal structure of the binder phase depends on the concentration of Ti and Hf that remain in the binder after cooling and the carbide phase in which the binder is embedded. Analysis of TEM electron diffraction patterns show that the binder phase consists of cubic solid solutions as well as intermetallic and cubic phases in which atomic ordering is observed.
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- Date Issued: 2007
Severe convective storm risk in the Eastern Cape Province of South Africa
- Authors: Pyle, Desmond Mark
- Date: 2007
- Subjects: Storms -- South Africa -- Eastern Cape Natural disasters -- South Africa -- Eastern Cape Disasters -- South Africa -- Eastern Cape Disaster relief -- South Africa -- Eastern Cape -- Management Meteorology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4853 , http://hdl.handle.net/10962/d1005529
- Description: This study investigates the temporal, spatial and impact characteristics of severe convective storm hazard and risk in the Eastern Cape Province of South Africa. Using historical data on severe convective storms dating from 1897, patterns of the hazard threat and risk to various geographic populations were investigated. A conceptual framework that emphasises the combined role hazard and vulnerability play in defining risk was used for the study. A methodology for ranking the severity of the storms in the historical dataset, based on recorded damage/impact, was specifically developed for the study. It is intended that this methodology will have a potentially wider application and may be adapted to a range of hazard impact and risk studies in South Africa and internationally. The study was undertaken within the context of the South African Disaster Management Act of 2002. Findings of the study show that severe convective storms can occur throughout the province, but there are clearly demarcated areas of higher frequency and concentration. The impact of storms is particularly severe on impoverished and vulnerable rural populations in the eastern parts of the province, where there is an urgent need for building capacity in disaster risk management. A major outcome of the study is the production of a severe convective storm hazard/risk map of the Eastern Cape, which it is hoped will be of benefit to a number of stakeholders in the province, particularly disaster management, but also the South African Weather Service, agricultural organisations, development/planning authorities, educational authorities and risk insurers. It is hoped that this map and the study in general will assist in guiding the operational responses of the various authorities, especially in terms of those interventions aimed at disaster risk reduction in the Eastern Cape.
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- Date Issued: 2007