“Pragmatic yet principled”: an assessment of Botswana’s Foreign Policy record as a small state
- Authors: Mahupela, Kabelo Moganegi
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/65290 , vital:28722
- Description: Expected release date-July 2019
- Full Text:
- Date Issued: 2014
- Authors: Mahupela, Kabelo Moganegi
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/65290 , vital:28722
- Description: Expected release date-July 2019
- Full Text:
- Date Issued: 2014
‘Unnatural’,‘un-African’and ‘ungodly’: homophobic discourse in democratic South Africa
- Vincent, Louise, Howell, Simon
- Authors: Vincent, Louise , Howell, Simon
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141570 , vital:37986 , DOI: 10.1177/1363460714524766
- Description: On 28 November 2006, South Africa’s legislature passed the Civil Union Act (No. 17, 2006), which legalised same-sex marriages or civil partnerships. While South Africa was fifth in the world to recognise the right of people of the same sex to marry, the country is by no means free of homophobic attitudes. However, we argue that an examination of the discourses embedded in the public discussion of gay marriage shows that the post-1994 period has not simply been a case of homophobia as usual. The altered context has given rise to the need for new ways of homophobic discourse to position itself. The rights enshrined in the Constitution represent a powerful set of ideas about the distinction between the democratic state and its apartheid predecessor. These ideas provide the dominant framework for political debate in the current context and it is therefore within this overarching framework that anti-gay sentiments must find a way to express themselves that has legitimacy. Our finding is that the reinscription of homophobia in an era of the ascendancy of human rights discourse has been chiefly in terms of three potent legitimising tropes – homosexuality as ‘unAfrican’, ‘unGodly’, and ‘unnatural’. It is only by deepening our understanding of the terms in which homophobia is being articulated in the current period that we can develop effective counter discourses.
- Full Text:
- Date Issued: 2014
- Authors: Vincent, Louise , Howell, Simon
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141570 , vital:37986 , DOI: 10.1177/1363460714524766
- Description: On 28 November 2006, South Africa’s legislature passed the Civil Union Act (No. 17, 2006), which legalised same-sex marriages or civil partnerships. While South Africa was fifth in the world to recognise the right of people of the same sex to marry, the country is by no means free of homophobic attitudes. However, we argue that an examination of the discourses embedded in the public discussion of gay marriage shows that the post-1994 period has not simply been a case of homophobia as usual. The altered context has given rise to the need for new ways of homophobic discourse to position itself. The rights enshrined in the Constitution represent a powerful set of ideas about the distinction between the democratic state and its apartheid predecessor. These ideas provide the dominant framework for political debate in the current context and it is therefore within this overarching framework that anti-gay sentiments must find a way to express themselves that has legitimacy. Our finding is that the reinscription of homophobia in an era of the ascendancy of human rights discourse has been chiefly in terms of three potent legitimising tropes – homosexuality as ‘unAfrican’, ‘unGodly’, and ‘unnatural’. It is only by deepening our understanding of the terms in which homophobia is being articulated in the current period that we can develop effective counter discourses.
- Full Text:
- Date Issued: 2014
‘Licking the snake’: the i'khothane and contemporary township youth identities in South Africa
- Howell, Simon, Vincent, Louise
- Authors: Howell, Simon , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141645 , vital:37993 , DOI: 10.1080/21528586.2014.917883
- Description: The i’khothane youth subculture is a relatively new phenomenon that has emerged in some of South Africa’s townships. Characterised by the rampant consumption of certain goods, such as expensive clothing, the subculture is unique in that it is also defined by the destruction of these goods in performances known as ‘battles’. Demonised by the media, we set out to explore what makes these practices meaningful to the participants themselves. On the basis of in-depth interviews conducted with the members of one group, we bring to the academic literature a scarcely analysed phenomenon that is nevertheless an acknowledged element of popular youth culture in contemporary South Africa. We attempt to place the practice of i’khothane within the context of the patterns of conspicuous consumption that have emerged in (a highly unequal) post-apartheid South Africa. While the practice of burning expensive consumer goods in public may seem alien, especially in contrast to the impoverished surroundings within which the i’khothane live, there are discernable and understandable reasons why the subculture has gained both popularity and notoriety. We show how the practice of i’khothane is a potent means of articulating youth identity in settings seemingly left behind by the ‘new’ South Africa.
- Full Text:
- Date Issued: 2014
- Authors: Howell, Simon , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141645 , vital:37993 , DOI: 10.1080/21528586.2014.917883
- Description: The i’khothane youth subculture is a relatively new phenomenon that has emerged in some of South Africa’s townships. Characterised by the rampant consumption of certain goods, such as expensive clothing, the subculture is unique in that it is also defined by the destruction of these goods in performances known as ‘battles’. Demonised by the media, we set out to explore what makes these practices meaningful to the participants themselves. On the basis of in-depth interviews conducted with the members of one group, we bring to the academic literature a scarcely analysed phenomenon that is nevertheless an acknowledged element of popular youth culture in contemporary South Africa. We attempt to place the practice of i’khothane within the context of the patterns of conspicuous consumption that have emerged in (a highly unequal) post-apartheid South Africa. While the practice of burning expensive consumer goods in public may seem alien, especially in contrast to the impoverished surroundings within which the i’khothane live, there are discernable and understandable reasons why the subculture has gained both popularity and notoriety. We show how the practice of i’khothane is a potent means of articulating youth identity in settings seemingly left behind by the ‘new’ South Africa.
- Full Text:
- Date Issued: 2014
Zimbabwe takes back its land:
- Authors: Helliker, Kirk D
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144677 , vital:38369 , DOI: 10.1080/02589001.2014.984946
- Description: Zimbabwe Takes Back its Land offers a useful introduction to fast-track land reform in contemporary Zimbabwe for a broad popular audience unfamiliar with the existing literature on fast-track land reform. But its value as a contribution to a more specialised and nuanced body of knowledge about fast-track is considerably more problematic.
- Full Text:
- Date Issued: 2014
- Authors: Helliker, Kirk D
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144677 , vital:38369 , DOI: 10.1080/02589001.2014.984946
- Description: Zimbabwe Takes Back its Land offers a useful introduction to fast-track land reform in contemporary Zimbabwe for a broad popular audience unfamiliar with the existing literature on fast-track land reform. But its value as a contribution to a more specialised and nuanced body of knowledge about fast-track is considerably more problematic.
- Full Text:
- Date Issued: 2014
Xenophobia, sovereign power and the limits of citizenship:
- Idahosa, Grace E, Vincent, Louise
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141970 , vital:38020 , DOI: 10.1080/09744053.2014.914637
- Description: African foreigners in South Africa have frequently been the targets of violent and discriminatory practices, which occur in the enabling context of negative discourses concerning African foreigners that circulate in various spheres of public life. This study is interested in one particular field of interaction between African foreigners and the South African state, namely the public health sector. Discriminatory and, sometimes, violent practices towards African foreigners on the part of South African citizens are widely documented. Less discussed are the ways in which these practices of violence and discrimination are in fact state practices. We show this with reference to the treatment of African foreigners in the public health sector. We refer to this prejudicial treatment as health-care Xenophobia which is made possible by a wider set of discourses related to citizenship and the rights accruing to citizens which suggest the ‘non-rights’ of the non-citizen.
- Full Text:
- Date Issued: 2014
- Authors: Idahosa, Grace E , Vincent, Louise
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141970 , vital:38020 , DOI: 10.1080/09744053.2014.914637
- Description: African foreigners in South Africa have frequently been the targets of violent and discriminatory practices, which occur in the enabling context of negative discourses concerning African foreigners that circulate in various spheres of public life. This study is interested in one particular field of interaction between African foreigners and the South African state, namely the public health sector. Discriminatory and, sometimes, violent practices towards African foreigners on the part of South African citizens are widely documented. Less discussed are the ways in which these practices of violence and discrimination are in fact state practices. We show this with reference to the treatment of African foreigners in the public health sector. We refer to this prejudicial treatment as health-care Xenophobia which is made possible by a wider set of discourses related to citizenship and the rights accruing to citizens which suggest the ‘non-rights’ of the non-citizen.
- Full Text:
- Date Issued: 2014
Writing the violated body : representations of violence against women in Margie Orford’s crime thriller novels
- Authors: Naidu, Samantha
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:26361 , http://hdl.handle.net/10962/53932 , http://dx.doi.org/10.1080/18125441.2014.904396 , https://orcid.org/0000-0001-9456-8657
- Description: Using the late twentieth-century French feminist notions of écriture féminine and the abject as a starting point, this article considers the various pitfalls, effects and ethical ramifications of representations of violence against the female body in South African crime fiction. How do authors reconcile the entertainment value of such representations with their aims to perform social analysis? This article attempts to answer this question by first describing how violence targeted at the female body is graphically portrayed, and, second, by assessing the effects of these visceral descriptions. Margie Orford’s novels, in particular, the first in the Clare Hart series, Like clockwork (2006), which foregrounds human trafficking, prostitution and gender-based violence, will be examined. In Orford’s Clare Hart series, the female detective figure, the various plots to do with assault, abduction, rape and murder, and the explicit imagery that descriptively conveys such crimes, are narrative techniques employed by Orford to address this scourge, and the patriarchy and sexism of contemporary South African society in general. The article ends by assessing whether a bona fide feminist subgenre of South African crime fiction is being inscribed by Orford
- Full Text: false
- Date Issued: 2014
- Authors: Naidu, Samantha
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:26361 , http://hdl.handle.net/10962/53932 , http://dx.doi.org/10.1080/18125441.2014.904396 , https://orcid.org/0000-0001-9456-8657
- Description: Using the late twentieth-century French feminist notions of écriture féminine and the abject as a starting point, this article considers the various pitfalls, effects and ethical ramifications of representations of violence against the female body in South African crime fiction. How do authors reconcile the entertainment value of such representations with their aims to perform social analysis? This article attempts to answer this question by first describing how violence targeted at the female body is graphically portrayed, and, second, by assessing the effects of these visceral descriptions. Margie Orford’s novels, in particular, the first in the Clare Hart series, Like clockwork (2006), which foregrounds human trafficking, prostitution and gender-based violence, will be examined. In Orford’s Clare Hart series, the female detective figure, the various plots to do with assault, abduction, rape and murder, and the explicit imagery that descriptively conveys such crimes, are narrative techniques employed by Orford to address this scourge, and the patriarchy and sexism of contemporary South African society in general. The article ends by assessing whether a bona fide feminist subgenre of South African crime fiction is being inscribed by Orford
- Full Text: false
- Date Issued: 2014
Why care about sharing?: Shared phones and shared netowrks in rural areas: African trends
- Authors: Dalvit, Lorenzo
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158639 , vital:40217 , https://hdl.handle.net/10520/EJC159490
- Description: Tomi Ahonen, credited with introducing the concept of mobile as the seventh mass media, notes that the arrival of the mobile phone was a God-send for advertisers, as it is the only mass medium where the audience can be accurately identified. Conversely, the pervasiveness of location-aware, multi-sensor, permanently on and constantly connected devices raised privacy concerns about carrying "little brother" in your pocket at all times. One of the distinctive characteristics of mobile phones, setting them apart from all previous media, is the fact that they are personal devices: 60% of married users would not let their spouse access their mobile phone and, not surprisingly, teenagers are even less inclined to let their family members have a look at their device. Things have not always been so. In South Africa, research conducted among university students revealed that for many a hand-me-down phone the size of a brick and shared with siblings was their first mobile device.
- Full Text:
- Date Issued: 2014
- Authors: Dalvit, Lorenzo
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158639 , vital:40217 , https://hdl.handle.net/10520/EJC159490
- Description: Tomi Ahonen, credited with introducing the concept of mobile as the seventh mass media, notes that the arrival of the mobile phone was a God-send for advertisers, as it is the only mass medium where the audience can be accurately identified. Conversely, the pervasiveness of location-aware, multi-sensor, permanently on and constantly connected devices raised privacy concerns about carrying "little brother" in your pocket at all times. One of the distinctive characteristics of mobile phones, setting them apart from all previous media, is the fact that they are personal devices: 60% of married users would not let their spouse access their mobile phone and, not surprisingly, teenagers are even less inclined to let their family members have a look at their device. Things have not always been so. In South Africa, research conducted among university students revealed that for many a hand-me-down phone the size of a brick and shared with siblings was their first mobile device.
- Full Text:
- Date Issued: 2014
Vulnerability, coping and adaptation within the context of climate change and HIV/AIDS in South Africa: Investigating strategies to strengthen livelihoods and food security and build resilience.
- Ndlovu, Patrick, Luckert, Martin K, Shackleton, Sheona E
- Authors: Ndlovu, Patrick , Luckert, Martin K , Shackleton, Sheona E
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:6622 , http://hdl.handle.net/10962/d1016230
- Description: [From Introduction] In South Africa, social grants are a central component of government's efforts to alleviate poverty. The number of people receiving social grants has significantly increased in recent years (from about 10.9 million in 2005 to almost 15.7 million in 2013, and an anticipated 16.8 million recipients by 2015).With social grants playing an increasingly important role, a pressing policy issue is whether or not the current social grant schemes are an effective tool for alleviating poverty. Some studies have shown that social grants improve food security (Case and Deaton, 1998; Samson et al., 2008) and in the long run can promote employment through accumulation of human capital and enhancing productivity of poor households (Edmonds et al., 2006; Samson et al., 2008; Surender et al., 2007). However, other studies have reported that social grants have possible disincentive effects on labor market activity, for example, through the relaxing of household budget constraints which may lead to a reduction in labor supply (Bertrand et al., 2003; Ranchorhod, 2006; Klasen and Woolard, 2009). Our study provides new insights by highlighting two key household characteristics, gender and education, in catalyzing or diminishing the effects of grants on household livelihood outcomes. Our analysis mainly focuses on impacts of pensions on household food security and labor supply of household members.
- Full Text:
- Date Issued: 2014
- Authors: Ndlovu, Patrick , Luckert, Martin K , Shackleton, Sheona E
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:6622 , http://hdl.handle.net/10962/d1016230
- Description: [From Introduction] In South Africa, social grants are a central component of government's efforts to alleviate poverty. The number of people receiving social grants has significantly increased in recent years (from about 10.9 million in 2005 to almost 15.7 million in 2013, and an anticipated 16.8 million recipients by 2015).With social grants playing an increasingly important role, a pressing policy issue is whether or not the current social grant schemes are an effective tool for alleviating poverty. Some studies have shown that social grants improve food security (Case and Deaton, 1998; Samson et al., 2008) and in the long run can promote employment through accumulation of human capital and enhancing productivity of poor households (Edmonds et al., 2006; Samson et al., 2008; Surender et al., 2007). However, other studies have reported that social grants have possible disincentive effects on labor market activity, for example, through the relaxing of household budget constraints which may lead to a reduction in labor supply (Bertrand et al., 2003; Ranchorhod, 2006; Klasen and Woolard, 2009). Our study provides new insights by highlighting two key household characteristics, gender and education, in catalyzing or diminishing the effects of grants on household livelihood outcomes. Our analysis mainly focuses on impacts of pensions on household food security and labor supply of household members.
- Full Text:
- Date Issued: 2014
Vice-Chancellor's 2014 Address to Graduation Ceremonies
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:7868 , http://hdl.handle.net/10962/d1016417
- Full Text:
- Date Issued: 2014
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:7868 , http://hdl.handle.net/10962/d1016417
- Full Text:
- Date Issued: 2014
Vice-Chancellor's 2014 Address to Graduation Ceremonies
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:7869 , http://hdl.handle.net/10962/d1016418
- Full Text:
- Date Issued: 2014
- Authors: Badat, Saleem
- Date: 2014
- Language: English
- Type: text
- Identifier: vital:7869 , http://hdl.handle.net/10962/d1016418
- Full Text:
- Date Issued: 2014
Using multivariate analysis and stable isotopes to assess the effects of substrate type on phytobenthos communities
- Dalu, Tatenda, Richoux, Nicole B, Froneman, P William
- Authors: Dalu, Tatenda , Richoux, Nicole B , Froneman, P William
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68123 , vital:29201 , https://doi.org/10.5268/IW-4.4.719
- Description: Publisher version , For more than a century, artificial substrates have been employed in phytobenthos studies. In the present study, we compared the phytobenthos community structure in a field experiment over 3 seasons (summer, autumn, and winter) on 3 types of artificial substrates (brick, brown clay tiles, and grey clay tiles) and 3 natural substrates (macrophytes, rocks, and sediment) in a small, temperate system. A combination of multivariate analyses (cluster, multi-response permutation procedure, indicator species [IndVal], and canonical correspondence analysis [CCA]) and stable isotope analysis was used. We identified 96 total phytobenthos taxa. Artificial substrates resulted in different substrate communities, as shown by stable isotope analysis, cluster analysis, and a multi-response permutation procedure, with only those communities growing on grey tiles being similar to natural substrate communities. Overall, artificial substrates exhibited slightly higher species richness compared to natural substrates over the 3 seasons, although there were no significant differences (p> 0.05). Phytobenthos grown on brown tiles, rocks, and bricks showed seasonal variability of the carbon isotope δ13C values using one-way ANOVA (p< 0.05). Phytobenthos community structure did not show great seasonal variation; however, CCA identified water flow, conductivity, ammonium, phosphate, and water depth as important in structuring phytobenthos communities on different substrates. IndVal analysis showed that common phytobenthos taxa were not restricted to a single substrate, but preference was generally high for natural substrate, especially rocks, compared to artificial substrates. Substrate microhabitat type seems to influence the communities within the study areas.
- Full Text: false
- Date Issued: 2014
- Authors: Dalu, Tatenda , Richoux, Nicole B , Froneman, P William
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68123 , vital:29201 , https://doi.org/10.5268/IW-4.4.719
- Description: Publisher version , For more than a century, artificial substrates have been employed in phytobenthos studies. In the present study, we compared the phytobenthos community structure in a field experiment over 3 seasons (summer, autumn, and winter) on 3 types of artificial substrates (brick, brown clay tiles, and grey clay tiles) and 3 natural substrates (macrophytes, rocks, and sediment) in a small, temperate system. A combination of multivariate analyses (cluster, multi-response permutation procedure, indicator species [IndVal], and canonical correspondence analysis [CCA]) and stable isotope analysis was used. We identified 96 total phytobenthos taxa. Artificial substrates resulted in different substrate communities, as shown by stable isotope analysis, cluster analysis, and a multi-response permutation procedure, with only those communities growing on grey tiles being similar to natural substrate communities. Overall, artificial substrates exhibited slightly higher species richness compared to natural substrates over the 3 seasons, although there were no significant differences (p> 0.05). Phytobenthos grown on brown tiles, rocks, and bricks showed seasonal variability of the carbon isotope δ13C values using one-way ANOVA (p< 0.05). Phytobenthos community structure did not show great seasonal variation; however, CCA identified water flow, conductivity, ammonium, phosphate, and water depth as important in structuring phytobenthos communities on different substrates. IndVal analysis showed that common phytobenthos taxa were not restricted to a single substrate, but preference was generally high for natural substrate, especially rocks, compared to artificial substrates. Substrate microhabitat type seems to influence the communities within the study areas.
- Full Text: false
- Date Issued: 2014
Unsymmetrically substituted nickel triazatetra-benzcorrole and phthalocynanine complexes
- Adegoke, Oluwasesan, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189818 , vital:44934 , xlink:href="https://doi.org/10.1007/s10895-013-1317-4"
- Description: We report on the design and application of fluorescent nanoprobes based on the covalent linking of L-glutathione-capped CdSe@ZnS quantum dots (QDs) to newly synthesized unsymmetrically substituted nickel mercaptosuccinic acid triazatetra-benzcorrole (3) and phthalocyanine (4) complexes. Fluorescence quenching of the QDs occurred on conjugation to complexes 3 or 4. The nanoprobes were selectively screened in the presence of different cations and Hg2+ showed excellent affinity in “turning ON” the fluorescence of the nanoprobes. Experimental results showed that the sensitivity of QDs-4 towards Hg2+ was much higher than that of QDs-3 nanoprobe. The mechanism of reaction has been elucidated based on the ability of Hg2+ to coordinate with the sulphur atom of the Ni complex ring and apparently “turn ON” the fluorescence of the linked QDs.
- Full Text:
- Date Issued: 2014
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189818 , vital:44934 , xlink:href="https://doi.org/10.1007/s10895-013-1317-4"
- Description: We report on the design and application of fluorescent nanoprobes based on the covalent linking of L-glutathione-capped CdSe@ZnS quantum dots (QDs) to newly synthesized unsymmetrically substituted nickel mercaptosuccinic acid triazatetra-benzcorrole (3) and phthalocyanine (4) complexes. Fluorescence quenching of the QDs occurred on conjugation to complexes 3 or 4. The nanoprobes were selectively screened in the presence of different cations and Hg2+ showed excellent affinity in “turning ON” the fluorescence of the nanoprobes. Experimental results showed that the sensitivity of QDs-4 towards Hg2+ was much higher than that of QDs-3 nanoprobe. The mechanism of reaction has been elucidated based on the ability of Hg2+ to coordinate with the sulphur atom of the Ni complex ring and apparently “turn ON” the fluorescence of the linked QDs.
- Full Text:
- Date Issued: 2014
Understanding the replication biology of Providence virus: elucidating the function of non-structural proteins
- Authors: Nakayinga, Ritah
- Date: 2014
- Subjects: Insects Viruses , Viruses Reproduction , Tombusviridae , RNA viruses , RNA polymerases
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/193930 , vital:45408
- Description: Tetraviruses are non-enveloped, small insect RNA viruses with a single stranded positive RNA genome that is either monopartite or bipartite. Providence virus (PrV) is the only member of the three tetravirus families with a viral replicase similar to the replicases of tombusviruses and umbraviruses. The principle aim of this thesis was to study PrV replication, focusing on subcellular localization and potential interactions between PrV replication proteins. The first objective of this study was to generate an anti-p104 antibody that does not cross-react with p40. Expression of the C-terminal portion of p104 in E. coli resulted in no detectable protein. Further expression in an insect cell based expression system resulted in the production of an insoluble protein. Attempts to improve protein solubility with a range of solubilization treatments were unsuccessful. Bioinformatic analysis was used to detect an antigenic region at the C-terminus of p104 and the peptide was used to raise anti-p104 antibodies. These antibodies did not detect native protein by western blot detection however they were used for immunoprecipitation. The establishment of the subcellular localization of PrV required two approaches; immunofluorescence in persistently infected Helicoverpa zea MG8 cells using antip40 and anti-dsRNA antibodies and the expression of EGFP-replicase fusion protein in Spodoptera frugiperda Sf9 cells. Replication of PrV was found to take place in cytosolic punctate structures. Co-immunoprecipitation experiments revealed that p40 self-interacts and interacts with p104. Bioinformatic analysis of PrV p104 suggests that the RdRp is similar to viral RdRps of the carmo-like supergroup II. Potential RNA binding regions are present within p104. A potential p40 interaction domain that shares hydrophilic and surface exposed properties with the TBSV p33 interaction domain is present. A putative arginine-rich region and disordered C-terminal region is present in p130. In conclusion, PrV p104 is the viral replicase. The resemblance of the expression strategy and putative functional domains with tombusviruses and umbraviruses suggest that PrV replication is related to the replication system of the tombusviruses and umbraviruses. This has led to propose that tetravirus replication strategies are diverse and raises questions on the origin and evolution of PrV. , Thesis (PhD) -- Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2014
- Full Text:
- Date Issued: 2014
- Authors: Nakayinga, Ritah
- Date: 2014
- Subjects: Insects Viruses , Viruses Reproduction , Tombusviridae , RNA viruses , RNA polymerases
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/193930 , vital:45408
- Description: Tetraviruses are non-enveloped, small insect RNA viruses with a single stranded positive RNA genome that is either monopartite or bipartite. Providence virus (PrV) is the only member of the three tetravirus families with a viral replicase similar to the replicases of tombusviruses and umbraviruses. The principle aim of this thesis was to study PrV replication, focusing on subcellular localization and potential interactions between PrV replication proteins. The first objective of this study was to generate an anti-p104 antibody that does not cross-react with p40. Expression of the C-terminal portion of p104 in E. coli resulted in no detectable protein. Further expression in an insect cell based expression system resulted in the production of an insoluble protein. Attempts to improve protein solubility with a range of solubilization treatments were unsuccessful. Bioinformatic analysis was used to detect an antigenic region at the C-terminus of p104 and the peptide was used to raise anti-p104 antibodies. These antibodies did not detect native protein by western blot detection however they were used for immunoprecipitation. The establishment of the subcellular localization of PrV required two approaches; immunofluorescence in persistently infected Helicoverpa zea MG8 cells using antip40 and anti-dsRNA antibodies and the expression of EGFP-replicase fusion protein in Spodoptera frugiperda Sf9 cells. Replication of PrV was found to take place in cytosolic punctate structures. Co-immunoprecipitation experiments revealed that p40 self-interacts and interacts with p104. Bioinformatic analysis of PrV p104 suggests that the RdRp is similar to viral RdRps of the carmo-like supergroup II. Potential RNA binding regions are present within p104. A potential p40 interaction domain that shares hydrophilic and surface exposed properties with the TBSV p33 interaction domain is present. A putative arginine-rich region and disordered C-terminal region is present in p130. In conclusion, PrV p104 is the viral replicase. The resemblance of the expression strategy and putative functional domains with tombusviruses and umbraviruses suggest that PrV replication is related to the replication system of the tombusviruses and umbraviruses. This has led to propose that tetravirus replication strategies are diverse and raises questions on the origin and evolution of PrV. , Thesis (PhD) -- Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2014
- Full Text:
- Date Issued: 2014
Understanding food security in a perfect storm: an ecosystem services approach
- Poppy, G M, Chiotha, S, Eigenbrod, Felix, Harvey, Celia A, Honza´k, M, Hudson, Malcolm D, Jarvis, A, Madise, Nyovani J, Schreckenberg, Kate, Shackleton, Charlie M, Villa, F, Dawson, Terrence P
- Authors: Poppy, G M , Chiotha, S , Eigenbrod, Felix , Harvey, Celia A , Honza´k, M , Hudson, Malcolm D , Jarvis, A , Madise, Nyovani J , Schreckenberg, Kate , Shackleton, Charlie M , Villa, F , Dawson, Terrence P
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/398423 , vital:69410 , xlink:href="https://doi.org/10.1098/rstb.2012.0288"
- Description: Achieving food security in a ‘perfect storm’ scenario is a grand challenge for society. Climate change and an expanding global population act in concert to make global food security even more complex and demanding. As achieving food security and the millennium development goal (MDG) to eradicate hunger influences the attainment of other MDGs, it is imperative that we offer solutions which are complementary and do not oppose one another. Sustainable intensification of agriculture has been proposed as a way to address hunger while also minimizing further environmental impact. However, the desire to raise productivity and yields has historically led to a degraded environment, reduced biodiversity and a reduction in ecosystem services (ES), with the greatest impacts affecting the poor. This paper proposes that the ES framework coupled with a policy response framework, for example Driver-Pressure-State-Impact-Response (DPSIR), can allow food security to be delivered alongside healthy ecosystems, which provide many other valuable services to humankind. Too often, agro-ecosystems have been considered as separate from other natural ecosystems and insufficient attention has been paid to the way in which services can flow to and from the agro-ecosystem to surrounding ecosystems. Highlighting recent research in a large multi-disciplinary project (ASSETS), we illustrate the ES approach to food security using a case study from the Zomba district of Malawi.
- Full Text:
- Date Issued: 2014
- Authors: Poppy, G M , Chiotha, S , Eigenbrod, Felix , Harvey, Celia A , Honza´k, M , Hudson, Malcolm D , Jarvis, A , Madise, Nyovani J , Schreckenberg, Kate , Shackleton, Charlie M , Villa, F , Dawson, Terrence P
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/398423 , vital:69410 , xlink:href="https://doi.org/10.1098/rstb.2012.0288"
- Description: Achieving food security in a ‘perfect storm’ scenario is a grand challenge for society. Climate change and an expanding global population act in concert to make global food security even more complex and demanding. As achieving food security and the millennium development goal (MDG) to eradicate hunger influences the attainment of other MDGs, it is imperative that we offer solutions which are complementary and do not oppose one another. Sustainable intensification of agriculture has been proposed as a way to address hunger while also minimizing further environmental impact. However, the desire to raise productivity and yields has historically led to a degraded environment, reduced biodiversity and a reduction in ecosystem services (ES), with the greatest impacts affecting the poor. This paper proposes that the ES framework coupled with a policy response framework, for example Driver-Pressure-State-Impact-Response (DPSIR), can allow food security to be delivered alongside healthy ecosystems, which provide many other valuable services to humankind. Too often, agro-ecosystems have been considered as separate from other natural ecosystems and insufficient attention has been paid to the way in which services can flow to and from the agro-ecosystem to surrounding ecosystems. Highlighting recent research in a large multi-disciplinary project (ASSETS), we illustrate the ES approach to food security using a case study from the Zomba district of Malawi.
- Full Text:
- Date Issued: 2014
Unclogging the wheels: how the shift from politics to law affects Africa's relationship with the international system
- Authors: Juma, Laurence
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/127123 , vital:35958 , https://heinonline.org/HOL/Page?handle=hein.journals/tlcp23amp;div=17amp;g_sent=1amp;casa_token=amp;collection=journals
- Description: Gone are the days when scholars debated whether international law was really law and whether the adjudicatory organs of the international system could effectuate the demands of the international rule of law. 1 This old time debate has, in recent times, given way to temperate and yet decisive acknowledgement of the legal authority of institutions resident within the international system.
- Full Text: false
- Date Issued: 2014
- Authors: Juma, Laurence
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/127123 , vital:35958 , https://heinonline.org/HOL/Page?handle=hein.journals/tlcp23amp;div=17amp;g_sent=1amp;casa_token=amp;collection=journals
- Description: Gone are the days when scholars debated whether international law was really law and whether the adjudicatory organs of the international system could effectuate the demands of the international rule of law. 1 This old time debate has, in recent times, given way to temperate and yet decisive acknowledgement of the legal authority of institutions resident within the international system.
- Full Text: false
- Date Issued: 2014
Troubling White Englishness in South Africa: a self-interrogation of privilege, complicity citizenship, and belonging
- Authors: Garman, Anthea
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/159746 , vital:40339 , ISBN 978-0739192962
- Description: Unveiling Whiteness in the Twenty-First Century: Global Manifestations, Transdisciplinary Interventions is a tightly interconnected and richly collaborative book that will advance our understanding of why it is so difficult to re-form and reimagine whiteness in the twenty-first century. Composed after the election of the first black U.S. president, post-global financial crisis, more than a decade after 9/11, and concomitant with a rash of xenophobic incidents across the globe, the book distills several key themes associated with a post-millennial global whiteness: the individual and collective emotions of whiteness, the recentering of whiteness through governing and legal strategies, and the retreats from social equity and justice that have characterized the late twentieth and twenty-first century nation state. It also attempts the difficult work of reimagining white identities and cultures for a new era.
- Full Text:
- Date Issued: 2014
- Authors: Garman, Anthea
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/159746 , vital:40339 , ISBN 978-0739192962
- Description: Unveiling Whiteness in the Twenty-First Century: Global Manifestations, Transdisciplinary Interventions is a tightly interconnected and richly collaborative book that will advance our understanding of why it is so difficult to re-form and reimagine whiteness in the twenty-first century. Composed after the election of the first black U.S. president, post-global financial crisis, more than a decade after 9/11, and concomitant with a rash of xenophobic incidents across the globe, the book distills several key themes associated with a post-millennial global whiteness: the individual and collective emotions of whiteness, the recentering of whiteness through governing and legal strategies, and the retreats from social equity and justice that have characterized the late twentieth and twenty-first century nation state. It also attempts the difficult work of reimagining white identities and cultures for a new era.
- Full Text:
- Date Issued: 2014
Trends in the TD-DFT calculations of porphyrin and phthalocyanine analogs
- Mack, John, Stone, Justin, Nyokong, Tebello
- Authors: Mack, John , Stone, Justin , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193882 , vital:45402 , xlink:href="https://doi.org/10.1142/S108842461450045X"
- Description: In 2005, Kobayashi and coworkers reported trends in the TD-DFT spectra of 17 Zn (II) porphyrinoids [J. Am. Chem. Soc. 2005; 127: 17697] that were analyzed using Michl's perimeter model as part of a study of the anomalous magnetic circular dichroism (MCD) spectroscopy of zinc tetraphenyltetraacenaphthoporphyrin. In recent years, it has become increasingly clear that TD-DFT calculations with the commonly used hybrid B3LYP exchange-correlation functional of the Gaussian software package are problematic in the B-band region of porphyrinoid spectra, since the degree of configurational interaction between the B and higher energy ππ* state appears to be significantly overestimated. The CAM-B3LYP functional is now often preferred for analyzing the optical properties of porphyrinoids, since it includes a long-range correction of the exchange potential, which incorporates an increasing fraction of Hartree–Fock (HF) exchange as the interelectronic separation increases, making it better suited for studying compounds where there is significant charge transfer in the electronic excited states. The trends in the TD-DFT calculations are reexamined with a wider range porphyrinoid compounds including several with pyrazino moieties and are found to provide a closer agreement with the experimental in the B-band region for complexes such as zinc tetraphenylporphyrin and phthalocyanine.
- Full Text:
- Date Issued: 2014
- Authors: Mack, John , Stone, Justin , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193882 , vital:45402 , xlink:href="https://doi.org/10.1142/S108842461450045X"
- Description: In 2005, Kobayashi and coworkers reported trends in the TD-DFT spectra of 17 Zn (II) porphyrinoids [J. Am. Chem. Soc. 2005; 127: 17697] that were analyzed using Michl's perimeter model as part of a study of the anomalous magnetic circular dichroism (MCD) spectroscopy of zinc tetraphenyltetraacenaphthoporphyrin. In recent years, it has become increasingly clear that TD-DFT calculations with the commonly used hybrid B3LYP exchange-correlation functional of the Gaussian software package are problematic in the B-band region of porphyrinoid spectra, since the degree of configurational interaction between the B and higher energy ππ* state appears to be significantly overestimated. The CAM-B3LYP functional is now often preferred for analyzing the optical properties of porphyrinoids, since it includes a long-range correction of the exchange potential, which incorporates an increasing fraction of Hartree–Fock (HF) exchange as the interelectronic separation increases, making it better suited for studying compounds where there is significant charge transfer in the electronic excited states. The trends in the TD-DFT calculations are reexamined with a wider range porphyrinoid compounds including several with pyrazino moieties and are found to provide a closer agreement with the experimental in the B-band region for complexes such as zinc tetraphenylporphyrin and phthalocyanine.
- Full Text:
- Date Issued: 2014
Tracing the ANC’s criticism of South African media: 20 years of democracy
- Authors: Malila, Vanessa
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158661 , vital:40219 , https://hdl.handle.net/10520/EJC159512
- Description: We often forget the strides that have been made in the media industry in South Africa since the end of apartheid and the repressive conditions under which the media industry operated prior to 1994. In the current context of complaints by the ANC about the lack of transformation in the industry and the poor reporting by the mainstream commercial media, the gains in ownership changes and the massive growth of the community media sector in South Africa are sometimes overshadowed. Despite a positive early relationship between the media and the ANC government, things have become progressively more difficult between these two institutions and the criticism from the ANC more vociferous in recent years.
- Full Text:
- Date Issued: 2014
- Authors: Malila, Vanessa
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158661 , vital:40219 , https://hdl.handle.net/10520/EJC159512
- Description: We often forget the strides that have been made in the media industry in South Africa since the end of apartheid and the repressive conditions under which the media industry operated prior to 1994. In the current context of complaints by the ANC about the lack of transformation in the industry and the poor reporting by the mainstream commercial media, the gains in ownership changes and the massive growth of the community media sector in South Africa are sometimes overshadowed. Despite a positive early relationship between the media and the ANC government, things have become progressively more difficult between these two institutions and the criticism from the ANC more vociferous in recent years.
- Full Text:
- Date Issued: 2014
Thinking Africa Newsletter: (March 2014)
- Authors: Matthews, Sally
- Date: 2014
- Language: English
- Type: text , article
- Identifier: vital:38067 , http://hdl.handle.net/20.500.12137/22
- Description: Thinking Africa Newsletter (March 2014).
- Full Text:
- Date Issued: 2014
- Authors: Matthews, Sally
- Date: 2014
- Language: English
- Type: text , article
- Identifier: vital:38067 , http://hdl.handle.net/20.500.12137/22
- Description: Thinking Africa Newsletter (March 2014).
- Full Text:
- Date Issued: 2014
Thinking Africa Newsletter: (June 2014)
- Authors: Matthews, Sally
- Date: 2014
- Language: English
- Type: text , article
- Identifier: vital:38065 , http://hdl.handle.net/20.500.12137/23
- Description: Thinking Africa Newsletter (June 2014).
- Full Text:
- Date Issued: 2014
- Authors: Matthews, Sally
- Date: 2014
- Language: English
- Type: text , article
- Identifier: vital:38065 , http://hdl.handle.net/20.500.12137/23
- Description: Thinking Africa Newsletter (June 2014).
- Full Text:
- Date Issued: 2014