Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
The high pressure catalytic hydrogenation of the tannin of black wattle (Acacia mollissima Willd)
- Authors: Silk, M H
- Date: 1947
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21132 , http://hdl.handle.net/10962/6528
- Description: From Introduction: The wattle industry in South Africa has, from its commencement in the middle of the last century, gradually assumed a position of increasing importance in the economic structure of the country. Apart from the provision of a valuable tanning extract for home use and for export, the tree itself has proved of considerable importance to the Union’s mining industries. In addition there would appear to be possibilities for the utilisation in the near future, of the raw ground bark and the wattle extract for the manufacture of a number of by-products, including certain types of plastics.
- Full Text:
- Date Issued: 1947
- Authors: Silk, M H
- Date: 1947
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:21132 , http://hdl.handle.net/10962/6528
- Description: From Introduction: The wattle industry in South Africa has, from its commencement in the middle of the last century, gradually assumed a position of increasing importance in the economic structure of the country. Apart from the provision of a valuable tanning extract for home use and for export, the tree itself has proved of considerable importance to the Union’s mining industries. In addition there would appear to be possibilities for the utilisation in the near future, of the raw ground bark and the wattle extract for the manufacture of a number of by-products, including certain types of plastics.
- Full Text:
- Date Issued: 1947
Risk management : a prerequisite for the implementation of government projects by the Eastern Cape Department of Local Government
- Authors: Sojini, Sandile
- Date: 2015
- Subjects: Risk management -- South Africa -- Eastern Cape Project management -- South Africa -- Eastern Cape Local government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2755 , vital:28073
- Description: This Research Report that this research was motivated by the fact that despite thetremendous achievements have been realized from the successful implementation ofgovernment programmes and projects related the construction and refurbishment of medical facilities, educational, bridges and road infrastructure facilities. Trends indicate that only iota of evidence implies that the concept of risk management is fully integrated and embraced as part of a culture for ensuring the successful project implementation by the Modern South African public sector organizations. Empirical research revealed that the common types of risks that have been affecting the successful implementation of government projects and programmes include: riots, fraud, corruption, changes in demographic figures, outsourcing risks, and floods and changes in weather. Most of the participants stated that risks associated with riots do not only arise from the riots within the government departments, but also in the external environment. In terms of the internal riots, they revealed that when the employees go on strike or riots on their own or in solidarity with the other trade unions, leading to the process for the implementation of government projects and programmes being affected. Yet, in certain cases, they noted that internal riots or strikes most occur as preceded by rumours that the riots by a particular union will take place and may significantly affect the ongoing process of implementation of government programmes. Besides risks associated with the internal riots by the employees and the communities, the interview findings also indicated that the other forms of risks arise from the strikes and riots by the private enterprises.
- Full Text:
- Date Issued: 2015
- Authors: Sojini, Sandile
- Date: 2015
- Subjects: Risk management -- South Africa -- Eastern Cape Project management -- South Africa -- Eastern Cape Local government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2755 , vital:28073
- Description: This Research Report that this research was motivated by the fact that despite thetremendous achievements have been realized from the successful implementation ofgovernment programmes and projects related the construction and refurbishment of medical facilities, educational, bridges and road infrastructure facilities. Trends indicate that only iota of evidence implies that the concept of risk management is fully integrated and embraced as part of a culture for ensuring the successful project implementation by the Modern South African public sector organizations. Empirical research revealed that the common types of risks that have been affecting the successful implementation of government projects and programmes include: riots, fraud, corruption, changes in demographic figures, outsourcing risks, and floods and changes in weather. Most of the participants stated that risks associated with riots do not only arise from the riots within the government departments, but also in the external environment. In terms of the internal riots, they revealed that when the employees go on strike or riots on their own or in solidarity with the other trade unions, leading to the process for the implementation of government projects and programmes being affected. Yet, in certain cases, they noted that internal riots or strikes most occur as preceded by rumours that the riots by a particular union will take place and may significantly affect the ongoing process of implementation of government programmes. Besides risks associated with the internal riots by the employees and the communities, the interview findings also indicated that the other forms of risks arise from the strikes and riots by the private enterprises.
- Full Text:
- Date Issued: 2015
An evaluation of the harmonised social cash transfer programme on livelihoods in Zimbabwe: the case of Mangwe District
- Authors: Poseni ,Pedzisai
- Date: 2019
- Subjects: Economic development -- Social aspects Social integration Social systems
- Language: English
- Type: Thesis , Masters , MCom (Development Studies)
- Identifier: http://hdl.handle.net/10353/13943 , vital:39730
- Description: Poverty and limited livelihood options remains a huge challenge in Africa, particularly in Zimbabwe. The study investigated the effectiveness of the Harmonized Social Cash Transfer programme in improving household economy and strengthening livelihood options in Zimbabwe with a particular focus on Mangwe District. In order to come up with a comprehensive investigation, the researcher grounded the study in sustainable livelihood approach and the capabaility approach. Due to persistent poverty and people adopting precarious coping strategies in Zimbawe, the government and NGOs collectively came up with a comprehensive cash based poverty alleviation strategy to alleviate poverty and build on peoples livelihood options which is the cash transfer programme. The study utilized a qualitative methodology, based on its ability to provide a contextualized description of people’s perspectives, feelings and opinions in terms of how a programme affected them. The evaluation sought to find out whether the cash transfer programme achieved its objective of strengthening household economy. The study established that the Harmonized Social Cash Transfer programme made some positive inroad towards strengthening household economy, however, lack of stakeholders coordination, collapsed economy, pollitically motivated moves from the ruling party caused the programme to fail. As such, the poor Mangwe people are still continuing engaging in precarious coping strategies, such as, selling assets, eating less than one meal per day and venturing into prostitution. The study therefore, calls for the government to review the household size vs cash scale and regularly review the amount of money received to be in line with current prices of commodities
- Full Text:
- Date Issued: 2019
- Authors: Poseni ,Pedzisai
- Date: 2019
- Subjects: Economic development -- Social aspects Social integration Social systems
- Language: English
- Type: Thesis , Masters , MCom (Development Studies)
- Identifier: http://hdl.handle.net/10353/13943 , vital:39730
- Description: Poverty and limited livelihood options remains a huge challenge in Africa, particularly in Zimbabwe. The study investigated the effectiveness of the Harmonized Social Cash Transfer programme in improving household economy and strengthening livelihood options in Zimbabwe with a particular focus on Mangwe District. In order to come up with a comprehensive investigation, the researcher grounded the study in sustainable livelihood approach and the capabaility approach. Due to persistent poverty and people adopting precarious coping strategies in Zimbawe, the government and NGOs collectively came up with a comprehensive cash based poverty alleviation strategy to alleviate poverty and build on peoples livelihood options which is the cash transfer programme. The study utilized a qualitative methodology, based on its ability to provide a contextualized description of people’s perspectives, feelings and opinions in terms of how a programme affected them. The evaluation sought to find out whether the cash transfer programme achieved its objective of strengthening household economy. The study established that the Harmonized Social Cash Transfer programme made some positive inroad towards strengthening household economy, however, lack of stakeholders coordination, collapsed economy, pollitically motivated moves from the ruling party caused the programme to fail. As such, the poor Mangwe people are still continuing engaging in precarious coping strategies, such as, selling assets, eating less than one meal per day and venturing into prostitution. The study therefore, calls for the government to review the household size vs cash scale and regularly review the amount of money received to be in line with current prices of commodities
- Full Text:
- Date Issued: 2019
The impact of inflation on financial development in South Africa
- Authors: Muzvanya, Kudzai Fungai
- Date: 2016
- Subjects: Economic development Monetary policy Consumer price indexes
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/13045 , vital:39440
- Description: Growing theoretical and empirical studies have predicted different influences that inflation has on financial development in different economies. This dissertation observes the impact South Africa’s inflation has on financial development over the period between 1990 and 2012. Monetary policy framework in South Africa has, to a greater extent, assisted in monitoring the movement of the consumer price index. Although inflation does affect financial sector performance, the study also looked into other variables that have an effect like private credit, money supply and gross domestic product. To test for stationarity to avoid spurious regression, the ADF test and the PP test were used. To determine the long- and short-run relationship, the Johansen Maximum Likelihood test and VECM models were used. Results of the study indicated that money supply and inflation have a negative effect on financial development. In addition, apart from money supply and inflation the findings revealed that private credit and gross domestic product play a significant part in financial sector performance. The study recommends that the South African Reserve Bank should keep the inflation rate within its target range (3-6percent). This would ensure price stability and restore investor confidence in the financial sector, which then improves financial sector development.
- Full Text:
- Date Issued: 2016
- Authors: Muzvanya, Kudzai Fungai
- Date: 2016
- Subjects: Economic development Monetary policy Consumer price indexes
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/13045 , vital:39440
- Description: Growing theoretical and empirical studies have predicted different influences that inflation has on financial development in different economies. This dissertation observes the impact South Africa’s inflation has on financial development over the period between 1990 and 2012. Monetary policy framework in South Africa has, to a greater extent, assisted in monitoring the movement of the consumer price index. Although inflation does affect financial sector performance, the study also looked into other variables that have an effect like private credit, money supply and gross domestic product. To test for stationarity to avoid spurious regression, the ADF test and the PP test were used. To determine the long- and short-run relationship, the Johansen Maximum Likelihood test and VECM models were used. Results of the study indicated that money supply and inflation have a negative effect on financial development. In addition, apart from money supply and inflation the findings revealed that private credit and gross domestic product play a significant part in financial sector performance. The study recommends that the South African Reserve Bank should keep the inflation rate within its target range (3-6percent). This would ensure price stability and restore investor confidence in the financial sector, which then improves financial sector development.
- Full Text:
- Date Issued: 2016
The relationship between household debt and consumption spending in South Africa (1994 - 2013)
- Authors: Nkala, Patience
- Date: 2016
- Subjects: Finance, Personal Financial services industry Consumption (Economics)
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/14022 , vital:39798
- Description: Consumption has been and remains the main contributor to gross domestic product (GDP) growth in South Africa. Household debt on the other side has remained high over the years. This study examined the relationship between household debt and consumption spending, for the period between 1994 and 2013. The Johansen cointegration technique and the Vector error correction model (VECM) were utilised to test the long run and short run relationships between the variables. The Granger causality test was also employed to test the direction of causality between the variables. Results from this study have revealed that a relationship exists between household debt and consumption spending in South Africa and they have also showed that this relationship flows from household debt to consumption spending. The implications of these results are that consumption spending may be increased through other measures rather than through increasing debt. The study therefore recommends that policy makers avail more investment opportunities for households and to also create employment in a bid to increase the income of households which can then be used to increase household consumption rather than the use of debt.
- Full Text:
- Date Issued: 2016
- Authors: Nkala, Patience
- Date: 2016
- Subjects: Finance, Personal Financial services industry Consumption (Economics)
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/14022 , vital:39798
- Description: Consumption has been and remains the main contributor to gross domestic product (GDP) growth in South Africa. Household debt on the other side has remained high over the years. This study examined the relationship between household debt and consumption spending, for the period between 1994 and 2013. The Johansen cointegration technique and the Vector error correction model (VECM) were utilised to test the long run and short run relationships between the variables. The Granger causality test was also employed to test the direction of causality between the variables. Results from this study have revealed that a relationship exists between household debt and consumption spending in South Africa and they have also showed that this relationship flows from household debt to consumption spending. The implications of these results are that consumption spending may be increased through other measures rather than through increasing debt. The study therefore recommends that policy makers avail more investment opportunities for households and to also create employment in a bid to increase the income of households which can then be used to increase household consumption rather than the use of debt.
- Full Text:
- Date Issued: 2016
Beef quality, oxidative stability and fatty acid composition of Nguni heifers fed diets with incremental levels of Opuntia ficus-indica (cactus) cladodes
- Authors: Mkhosi, Silungile
- Date: 2020
- Subjects: Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc (Animal Science )
- Identifier: http://hdl.handle.net/10353/18732 , vital:42728
- Description: The objective of the study was to determine meat quality, fatty acid (FA) composition and shelf-life stability of longissimus muscle of Nguni heifers fed incremental levels of Opuntia ficus-indica (cactus) cladodes. A total of 32 heifers aged 36 months were allocated randomly to four dietary treatments: 10% cactus, 20% cactus, commercial and control diets for 90 days. The longissimus thoracis et lumborum (LTL) samples were taken from each carcass for the determination of meat quality, retail shelf-life stability and FA composition. Diet had no effect (P > 0.05) on pH and drip display, however, there was an increase in drip display with ageing time (P ≤ 0.05). Meat colour attributes (L* a* b* and C*) were influenced by diet, with 20% cactus diet having lower L* values compared to other diets (P ≤ 0.05). During a 14-day retail display, a diet day interaction was observed for hue angle with the cactus diets having the lowest values at day 14 of display compared to the other diets (P ≤ 0.05). Differences in meat from heifers fed different diets were observed with 20% cactus accounting for significantly (P ≤ 0.05) higher levels of deoxymyoglobin and the lowest levels of oxymyoglobin. Thawing loss and Warner Bratzler shear force decreased (P ≤ 0.05) with ageing period. Meat from heifers fed cactus diets had lower (P ≤ 0.05) proportions of oleic acid (C18:1n9c) and total MUFA, higher (P ≤ 0.05) proportions of total SFA, C15:1, C17:1, linoleic acid (C18:2n6c) and total PUFA. The control diet produced meat with the highest thiobarbituric acid reactive substances (TBARS) values compared to other diets (P ≤ 0.05) and with ageing TBARS values increased (P ≤ 0.05). Diet showed an effect (P ≤ 0.05) on sensory attributes such as overall tenderness and mouth feel with cactus diets accounting for lower scores. However, juiciness, overall tenderness, grassy and animal like attributes increased with ageing (P ≤ 0.05). Inclusion of cactus in the diet did not produce any adverse effects on meat quality, fatty acid composition, lipid oxidation and sensory quality. Therefore, it can be concluded that inclusion of cactus in Nguni heifer finisher diets up to 20% can improve meat quality.
- Full Text:
- Date Issued: 2020
- Authors: Mkhosi, Silungile
- Date: 2020
- Subjects: Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc (Animal Science )
- Identifier: http://hdl.handle.net/10353/18732 , vital:42728
- Description: The objective of the study was to determine meat quality, fatty acid (FA) composition and shelf-life stability of longissimus muscle of Nguni heifers fed incremental levels of Opuntia ficus-indica (cactus) cladodes. A total of 32 heifers aged 36 months were allocated randomly to four dietary treatments: 10% cactus, 20% cactus, commercial and control diets for 90 days. The longissimus thoracis et lumborum (LTL) samples were taken from each carcass for the determination of meat quality, retail shelf-life stability and FA composition. Diet had no effect (P > 0.05) on pH and drip display, however, there was an increase in drip display with ageing time (P ≤ 0.05). Meat colour attributes (L* a* b* and C*) were influenced by diet, with 20% cactus diet having lower L* values compared to other diets (P ≤ 0.05). During a 14-day retail display, a diet day interaction was observed for hue angle with the cactus diets having the lowest values at day 14 of display compared to the other diets (P ≤ 0.05). Differences in meat from heifers fed different diets were observed with 20% cactus accounting for significantly (P ≤ 0.05) higher levels of deoxymyoglobin and the lowest levels of oxymyoglobin. Thawing loss and Warner Bratzler shear force decreased (P ≤ 0.05) with ageing period. Meat from heifers fed cactus diets had lower (P ≤ 0.05) proportions of oleic acid (C18:1n9c) and total MUFA, higher (P ≤ 0.05) proportions of total SFA, C15:1, C17:1, linoleic acid (C18:2n6c) and total PUFA. The control diet produced meat with the highest thiobarbituric acid reactive substances (TBARS) values compared to other diets (P ≤ 0.05) and with ageing TBARS values increased (P ≤ 0.05). Diet showed an effect (P ≤ 0.05) on sensory attributes such as overall tenderness and mouth feel with cactus diets accounting for lower scores. However, juiciness, overall tenderness, grassy and animal like attributes increased with ageing (P ≤ 0.05). Inclusion of cactus in the diet did not produce any adverse effects on meat quality, fatty acid composition, lipid oxidation and sensory quality. Therefore, it can be concluded that inclusion of cactus in Nguni heifer finisher diets up to 20% can improve meat quality.
- Full Text:
- Date Issued: 2020
The influence of technology factors on turnover intentions amongst a sample of government employees in the Eastern Cape, South Africa
- Authors: Mahlasela, Samkelisiwe
- Date: 2018
- Subjects: Work -- Technological innovations
- Language: English
- Type: Thesis , Masters , MCom (Industrial Psychology)
- Identifier: http://hdl.handle.net/10353/13535 , vital:39677
- Description: Orientation: Technology affects organisations both positively and negatively and in some instances technology factors can result in turnover intentions for certain employees of organisations. Research purpose: The main objective of the study was to investigate how technology-based job autonomy, technology-based job overload and technologybased job monitoring impact turnover intentions of employees. Motivation of the study: Exploring and understanding the relationship between technology factors and turnover intention amongst government employees to determine whether these factors affect government as much as they affect the private sector. Research design, approach and method: This research used a quantitative research approach. It drew a convenience sample of 182 employees (N= 182) from the South African Government. Main findings: The three main findings of the study were: Technology-based job autonomy was positively related to turnover intention. Technology-based job overload was found to be positively related to turnover intention. Finally, technology-based monitoring was found to be positively related to turnover intention. Practical/managerial implications: If organisations do not address the issue of monitoring and overload they are likely to experience many turnover intentions amongst the employees. Furthermore, the organisations with less autonomy are likely to lose their employees which means they will lose the resources that have been serving the organisation for a long time. This will also contribute to the organisation losing many funds as they would constantly have to train new employees. Contribution/value-add: The research adds value to the knowledge about the effects of technology factors on turnover intention by pointing out the reasons that lead to turnover intention.
- Full Text:
- Date Issued: 2018
- Authors: Mahlasela, Samkelisiwe
- Date: 2018
- Subjects: Work -- Technological innovations
- Language: English
- Type: Thesis , Masters , MCom (Industrial Psychology)
- Identifier: http://hdl.handle.net/10353/13535 , vital:39677
- Description: Orientation: Technology affects organisations both positively and negatively and in some instances technology factors can result in turnover intentions for certain employees of organisations. Research purpose: The main objective of the study was to investigate how technology-based job autonomy, technology-based job overload and technologybased job monitoring impact turnover intentions of employees. Motivation of the study: Exploring and understanding the relationship between technology factors and turnover intention amongst government employees to determine whether these factors affect government as much as they affect the private sector. Research design, approach and method: This research used a quantitative research approach. It drew a convenience sample of 182 employees (N= 182) from the South African Government. Main findings: The three main findings of the study were: Technology-based job autonomy was positively related to turnover intention. Technology-based job overload was found to be positively related to turnover intention. Finally, technology-based monitoring was found to be positively related to turnover intention. Practical/managerial implications: If organisations do not address the issue of monitoring and overload they are likely to experience many turnover intentions amongst the employees. Furthermore, the organisations with less autonomy are likely to lose their employees which means they will lose the resources that have been serving the organisation for a long time. This will also contribute to the organisation losing many funds as they would constantly have to train new employees. Contribution/value-add: The research adds value to the knowledge about the effects of technology factors on turnover intention by pointing out the reasons that lead to turnover intention.
- Full Text:
- Date Issued: 2018
An exploration of constructions of masculinity : a narrative study of young Zulu men's stories of 'being a man'
- Authors: Maxwell, Justin Kennedy
- Date: 2005
- Subjects: Masculinity -- South Africa -- KwaZulu-Natal , Men -- South Africa -- Psychology , Discourse analysis, Narrative -- Psychological aspects , Zulu (African people) -- Social life and customs , Men -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3251 , http://hdl.handle.net/10962/d1015763
- Description: Previously understood as a fixed and universal set of behaviours social constructionists are now arguing that masculinity is contextual and fluid, reflecting a multiplicity of different understandings. Within any 'cultural environment' the discourse of masculinity, culturally and historically bound, expresses attitudes and behaviours that shape the understanding of what it means to be a man. Adopting a narrative approach and analysis this research explores the stories of six Zulu men in seeking to elicit the aspects of their masculinity and show how these men negotiate an identity 'position' from the social narratives available to them. It was found that while the ideal Adult (responsible) man contrasts with the Young man's ('isoka' ) position there is a consistently hegemonic and patriarchal notion of masculinity.
- Full Text:
- Date Issued: 2005
- Authors: Maxwell, Justin Kennedy
- Date: 2005
- Subjects: Masculinity -- South Africa -- KwaZulu-Natal , Men -- South Africa -- Psychology , Discourse analysis, Narrative -- Psychological aspects , Zulu (African people) -- Social life and customs , Men -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:3251 , http://hdl.handle.net/10962/d1015763
- Description: Previously understood as a fixed and universal set of behaviours social constructionists are now arguing that masculinity is contextual and fluid, reflecting a multiplicity of different understandings. Within any 'cultural environment' the discourse of masculinity, culturally and historically bound, expresses attitudes and behaviours that shape the understanding of what it means to be a man. Adopting a narrative approach and analysis this research explores the stories of six Zulu men in seeking to elicit the aspects of their masculinity and show how these men negotiate an identity 'position' from the social narratives available to them. It was found that while the ideal Adult (responsible) man contrasts with the Young man's ('isoka' ) position there is a consistently hegemonic and patriarchal notion of masculinity.
- Full Text:
- Date Issued: 2005
The mandibular gland secretions of the Cape honeybee (Apis mellifera capensis ESCH.) : factors affecting the production of the chemical signal and implications for further development of beekeeping in South Africa
- Authors: Jones, Georgina Elizabeth
- Date: 2001
- Subjects: Honeybee Honeybee -- Physiology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5730 , http://hdl.handle.net/10962/d1005416
- Description: The chemical composition of the mandibular gland extracts of Apis mellifera capensis virgin queens was analysed by gas chromatography-mass spectroscopy. Thirty-seven compounds from various chemical groups including aliphatic and aromatic acids and diacids, phenols, alkanes, amino acids and sugars were identified. Among the identified compounds were the queen mandibular pheromone components 9ODA, 9HDA, HVA and HOB and the other aliphatic acids and phenols considered to be the major components of A.m. capensis mandibular glands. Ontogenetic changes in the concentration of the mandibular gland secretions of virgin queens were largely quantitative in nature with the total volume and that of most of the compounds increasing with queen age. The final level of 9ODA is reached at the premating stage, approximately three days after emergence, when it comprises approximately 87% of the major constituents of the mandibular gland signal. Hostile reactions by workers towards introduced virgin queens can be correlated to the relative proportion of 9ODA present in the mandibular gland secretions. This seems to indicate that it is the complete spectrum of the signal and not individual compounds that determine worker reaction towards introduced queens. Keeping queens singly, with or without workers, in an incubator and in small mating nucleus hives proved to be the most successful methods of queen rearing in respect to survival rate in A.m. capensis. The presence of workers during the ageing of virgin queens was found to significantly affect the chemical composition of the mandibular gland secretions of queens. The reaction of workers towards introduced virgin queens reared under different holding conditions varied, with queens reared with workers eliciting significantly less hostile reactions from workers than those reared without workers. Mated queens from five localities in the Eastern Cape were characterised on the basis of the chemical composition of their mandibular gland secretions and the ratio of 9ODA:10HDA. No significant differences were detected and none of the queens sampled could be considered to be A.m. capensis based on their mandibular gland signal. The findings of this study provide baseline data for the development of a queen-rearing program tailored to the specific requirements of A.m. capensis.
- Full Text:
- Date Issued: 2001
- Authors: Jones, Georgina Elizabeth
- Date: 2001
- Subjects: Honeybee Honeybee -- Physiology
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5730 , http://hdl.handle.net/10962/d1005416
- Description: The chemical composition of the mandibular gland extracts of Apis mellifera capensis virgin queens was analysed by gas chromatography-mass spectroscopy. Thirty-seven compounds from various chemical groups including aliphatic and aromatic acids and diacids, phenols, alkanes, amino acids and sugars were identified. Among the identified compounds were the queen mandibular pheromone components 9ODA, 9HDA, HVA and HOB and the other aliphatic acids and phenols considered to be the major components of A.m. capensis mandibular glands. Ontogenetic changes in the concentration of the mandibular gland secretions of virgin queens were largely quantitative in nature with the total volume and that of most of the compounds increasing with queen age. The final level of 9ODA is reached at the premating stage, approximately three days after emergence, when it comprises approximately 87% of the major constituents of the mandibular gland signal. Hostile reactions by workers towards introduced virgin queens can be correlated to the relative proportion of 9ODA present in the mandibular gland secretions. This seems to indicate that it is the complete spectrum of the signal and not individual compounds that determine worker reaction towards introduced queens. Keeping queens singly, with or without workers, in an incubator and in small mating nucleus hives proved to be the most successful methods of queen rearing in respect to survival rate in A.m. capensis. The presence of workers during the ageing of virgin queens was found to significantly affect the chemical composition of the mandibular gland secretions of queens. The reaction of workers towards introduced virgin queens reared under different holding conditions varied, with queens reared with workers eliciting significantly less hostile reactions from workers than those reared without workers. Mated queens from five localities in the Eastern Cape were characterised on the basis of the chemical composition of their mandibular gland secretions and the ratio of 9ODA:10HDA. No significant differences were detected and none of the queens sampled could be considered to be A.m. capensis based on their mandibular gland signal. The findings of this study provide baseline data for the development of a queen-rearing program tailored to the specific requirements of A.m. capensis.
- Full Text:
- Date Issued: 2001
Using the local environment for outcomes-based education: issues raised for INSET by teachers' perceptions of agents of change
- Authors: Burton, Noreen
- Date: 1998
- Subjects: In-service education and training for teachers Teachers -- Training of -- South Africa Teachers -- In-service training -- South Africa Competency-based education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1818 , http://hdl.handle.net/10962/d1003704
- Description: South African education is in the process of undergoing what has become known as a "paradigm shift", requiring a change from the familiar transmission method to outcomes-based education (OBE) founded on social constructivist theories oflearning and teaching. Teachers, (45% of whom are poorly qualified), "will play a pivotal role in the success or failure of this change. There is therefore an urgent need to re-educate in-service teachers in the philosophy, and related methodologies underpinning Curriculum 2005. To this end, in-service education courses need to be developed "which will encourage teachers to reconstruct their beliefs about teaching and learning. INSET programmes in the past were aimed at either "topping up" qualified teachers, or "adding on" for under qualified teachers - neither of which have proved successful. It is therefore unlikely that applying similar methodologies to support teachers in the shift to OBE will meet with success, nor will simply "training" teachers to use the new learning programmes. Government documents on Education Policy are calling for teachers to become reflexive practitioners, able to guide learners in their efforts to achieve the critical outcomes which are intended to develop a competent citizenry of lifelong learners. Therefore, teachers ought to be empowered as curriculum developers in their own right. This interpretative case study hoped to find out from teachers themselves what aspects of an activity-based environmental education course acted as change agents in assisting them to begin the shift to constructivist theories of teaching and learning. A variety of research tools were used, including questionnaires, interviews, journals, narratives and concept maps, all of which provided a rich source of data for interpretation. From what was gained from four participants in this research, tentative suggestions for incorporation into the next cycle of action research in future INSET programmes are made.
- Full Text:
- Date Issued: 1998
- Authors: Burton, Noreen
- Date: 1998
- Subjects: In-service education and training for teachers Teachers -- Training of -- South Africa Teachers -- In-service training -- South Africa Competency-based education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1818 , http://hdl.handle.net/10962/d1003704
- Description: South African education is in the process of undergoing what has become known as a "paradigm shift", requiring a change from the familiar transmission method to outcomes-based education (OBE) founded on social constructivist theories oflearning and teaching. Teachers, (45% of whom are poorly qualified), "will play a pivotal role in the success or failure of this change. There is therefore an urgent need to re-educate in-service teachers in the philosophy, and related methodologies underpinning Curriculum 2005. To this end, in-service education courses need to be developed "which will encourage teachers to reconstruct their beliefs about teaching and learning. INSET programmes in the past were aimed at either "topping up" qualified teachers, or "adding on" for under qualified teachers - neither of which have proved successful. It is therefore unlikely that applying similar methodologies to support teachers in the shift to OBE will meet with success, nor will simply "training" teachers to use the new learning programmes. Government documents on Education Policy are calling for teachers to become reflexive practitioners, able to guide learners in their efforts to achieve the critical outcomes which are intended to develop a competent citizenry of lifelong learners. Therefore, teachers ought to be empowered as curriculum developers in their own right. This interpretative case study hoped to find out from teachers themselves what aspects of an activity-based environmental education course acted as change agents in assisting them to begin the shift to constructivist theories of teaching and learning. A variety of research tools were used, including questionnaires, interviews, journals, narratives and concept maps, all of which provided a rich source of data for interpretation. From what was gained from four participants in this research, tentative suggestions for incorporation into the next cycle of action research in future INSET programmes are made.
- Full Text:
- Date Issued: 1998
The large scale bioinformatics analysis of auxiliary activity family 9 enzymes
- Authors: Moses, Vuyani
- Date: 2014
- Subjects: Bioinformatics -- Analysis , Cellulose -- Biodegradation , Biomass energy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4145 , http://hdl.handle.net/10962/d1016356
- Description: Biofuels have been proposed to be a suitable replacement to the already depleting fossil fuels. The complex structures of plant biomasses present a challenge the production of biofuels due to recalcitrance. The complex cellulose structure and hydrogen bonding between repeat units of cellulose is believed to be a major contributor to the recalcitrance of cellulose. Fungal organisms come equipped with various oxidative enzymes involved in degradation of plant biomass. The exact mechanism of cellulose degradation remains elusive. The GH61 is a group of proteins which are PMOs. GH61 sequences where previously described as endoglucanases due to weak endoglucanase activity. These enzymes were later found not possess any enzyme activity of their own however they could enhance the activity of other cellulose degrading enzymes. As a result reclassification of these enzymes as AA9 has been implemented. AA9 proteins have been reported to share structural homology with the bacterial AA10 group of enzymes. Based on cleavage products that are produced when AA9 proteins interact with cellulose, AA9 proteins have been grouped into three types. To date the exact mechanism and the sequence and structural basis for differentiating between the various AA9 types remains unknown. Using various bionformatic techniques sequence and structural elements were identified for distinguishing between the AA9 types. A large dataset of sequences was obtained from the Pfam database from UNIPROT entries. Due to high divergence of AA9 sequences, a smaller dataset with the more divergent sequences removed was created. The inclusion of the reference sequences to the data set was done to observe which sequences belong to a certain type. Phylogenetic analysis was able to group AA9 proteins into three distinct groups. MSA and motif analysis revealed that the N-Terminus of these proteins is mostly responsible for type specificity. Structural analysis of AA9 PDB structures and homology models allowed the effect of physicochemical properties to be gauged structurally. The presence of 310 helices and aromatic residues the surface of AA9 sequences is an observation which still warrants further investigation.
- Full Text:
- Date Issued: 2014
- Authors: Moses, Vuyani
- Date: 2014
- Subjects: Bioinformatics -- Analysis , Cellulose -- Biodegradation , Biomass energy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4145 , http://hdl.handle.net/10962/d1016356
- Description: Biofuels have been proposed to be a suitable replacement to the already depleting fossil fuels. The complex structures of plant biomasses present a challenge the production of biofuels due to recalcitrance. The complex cellulose structure and hydrogen bonding between repeat units of cellulose is believed to be a major contributor to the recalcitrance of cellulose. Fungal organisms come equipped with various oxidative enzymes involved in degradation of plant biomass. The exact mechanism of cellulose degradation remains elusive. The GH61 is a group of proteins which are PMOs. GH61 sequences where previously described as endoglucanases due to weak endoglucanase activity. These enzymes were later found not possess any enzyme activity of their own however they could enhance the activity of other cellulose degrading enzymes. As a result reclassification of these enzymes as AA9 has been implemented. AA9 proteins have been reported to share structural homology with the bacterial AA10 group of enzymes. Based on cleavage products that are produced when AA9 proteins interact with cellulose, AA9 proteins have been grouped into three types. To date the exact mechanism and the sequence and structural basis for differentiating between the various AA9 types remains unknown. Using various bionformatic techniques sequence and structural elements were identified for distinguishing between the AA9 types. A large dataset of sequences was obtained from the Pfam database from UNIPROT entries. Due to high divergence of AA9 sequences, a smaller dataset with the more divergent sequences removed was created. The inclusion of the reference sequences to the data set was done to observe which sequences belong to a certain type. Phylogenetic analysis was able to group AA9 proteins into three distinct groups. MSA and motif analysis revealed that the N-Terminus of these proteins is mostly responsible for type specificity. Structural analysis of AA9 PDB structures and homology models allowed the effect of physicochemical properties to be gauged structurally. The presence of 310 helices and aromatic residues the surface of AA9 sequences is an observation which still warrants further investigation.
- Full Text:
- Date Issued: 2014
Production of recombinant human CD21 and CD23 : towards a better understanding of their interaction
- Authors: Van Zyl, Dwain George
- Date: 2013
- Subjects: Immunoglobulins , Fc receptors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10365 , http://hdl.handle.net/10948/d10211135
- Description: The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world suffers from allergic diseases. The majority of allergic diseases are rooted in the activities of IgE; an immunoglobulin which exerts its effector functions by interacting with a network of proteins. This network includes its low affinity receptor CD23. Cross linking of membrane IgE and CD21 by soluble CD23 results in an increase in IgE synthesis. This marks the interaction between CD23 and CD21 as an attractive therapeutic target. However, details regarding this interaction are inadequate for rational drug design. To obtain a deeper understanding of the CD23-CD21 interaction recombinant human CD21 (SCR1-2 and SCR5-8) and CD23 (16 kD and 25 kDa) were produced. The cloning, expression and purification of recombinant proteins comprised a significant portion of this study. Recombinant CD23 was expressed as inclusion bodies, refolded by rapid dilution and purified by size exclusion chromatography. Conversely, recombinant CD21 was expressed as soluble MBP-fusions and purified with an amylose affinity resin. The interaction between recombinant CD23 and CD21 was analysed by flow cytometry and ELISA experiments. Flow cytometry showed that 16 kDa and 25 kDa CD23 interacted with SCR5-8 to the same extent. Semi-quantitative ELISA experiments showed that both SCR1-2 and SCR5-8 were able to interact with 16 kDa and 25 kDa CD23. This suggests that the binding sites of SCR1-2 and SCR5-8 occur on 16 kDa CD23. Furthermore, since proteins were expressed in E. coli it suggests that the CD23-CD21 interaction does not require glycosylation. Furthermore, considering what is known about the SCR1-2-CD23 interaction from previous NMR studies; i.e. that the C-terminal tail (residues residues 289-298) of CD23 is responsible for binding SCR1-2, indicates that SCR5-8 binds somewhere within the lectin domain of CD23. This indicates that the CD23-CD21 interaction involves C-terminal tail-SCR1-2 and lectin domain-SCR5-8 interactions.
- Full Text:
- Date Issued: 2013
- Authors: Van Zyl, Dwain George
- Date: 2013
- Subjects: Immunoglobulins , Fc receptors
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10365 , http://hdl.handle.net/10948/d10211135
- Description: The prevalence of allergic diseases has dramatically increased over the last three decades. Presently, it is estimated that 20-30 per cent of the developed world suffers from allergic diseases. The majority of allergic diseases are rooted in the activities of IgE; an immunoglobulin which exerts its effector functions by interacting with a network of proteins. This network includes its low affinity receptor CD23. Cross linking of membrane IgE and CD21 by soluble CD23 results in an increase in IgE synthesis. This marks the interaction between CD23 and CD21 as an attractive therapeutic target. However, details regarding this interaction are inadequate for rational drug design. To obtain a deeper understanding of the CD23-CD21 interaction recombinant human CD21 (SCR1-2 and SCR5-8) and CD23 (16 kD and 25 kDa) were produced. The cloning, expression and purification of recombinant proteins comprised a significant portion of this study. Recombinant CD23 was expressed as inclusion bodies, refolded by rapid dilution and purified by size exclusion chromatography. Conversely, recombinant CD21 was expressed as soluble MBP-fusions and purified with an amylose affinity resin. The interaction between recombinant CD23 and CD21 was analysed by flow cytometry and ELISA experiments. Flow cytometry showed that 16 kDa and 25 kDa CD23 interacted with SCR5-8 to the same extent. Semi-quantitative ELISA experiments showed that both SCR1-2 and SCR5-8 were able to interact with 16 kDa and 25 kDa CD23. This suggests that the binding sites of SCR1-2 and SCR5-8 occur on 16 kDa CD23. Furthermore, since proteins were expressed in E. coli it suggests that the CD23-CD21 interaction does not require glycosylation. Furthermore, considering what is known about the SCR1-2-CD23 interaction from previous NMR studies; i.e. that the C-terminal tail (residues residues 289-298) of CD23 is responsible for binding SCR1-2, indicates that SCR5-8 binds somewhere within the lectin domain of CD23. This indicates that the CD23-CD21 interaction involves C-terminal tail-SCR1-2 and lectin domain-SCR5-8 interactions.
- Full Text:
- Date Issued: 2013
Through our eyes: teachers using cameras to engage in environmental education curriculum development processes
- Authors: Du Toit, Derick
- Date: 1999
- Subjects: Environmental education -- South Africa Curriculum change -- South Africa Photography in education Competency-based education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1800 , http://hdl.handle.net/10962/d1003685
- Description: This research explores the potential for engaging teachers in school-based environmental education curriculum development processes by using camera. The research, through its epistemological and ontological position, is closely linked to educational orientations associated with aspects of outcomes-based educational transformation in South Africa. A participatory approach that recognises teachers as co-researchers, each bringing to the inquiry her or his questions and constructions of meaning, was adopted. Participatory inquiry was initiated by setting up cluster meetings that allowed for teacher inputs through open dialogic processes. Fundamental to the inquiry is the notion that context shapes curriculum and curriculum development processes. It was from this orientation that a group of 13 teachers, using cameras to create visual narratives, explored their own diverse and complex contexts. These narratives (or stories) form the basis for further inquiry and development of sophistication with respect to the concept of environment. The research process is critically and reflexively documented as a series of field and research texts constructed from a variety of data sources gathered over the period of one year. The work is presented as a process of engaging critically with environmental education curriculum development and an opportunity to raise questions, rather than seek answers in this regard.
- Full Text:
- Date Issued: 1999
- Authors: Du Toit, Derick
- Date: 1999
- Subjects: Environmental education -- South Africa Curriculum change -- South Africa Photography in education Competency-based education -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1800 , http://hdl.handle.net/10962/d1003685
- Description: This research explores the potential for engaging teachers in school-based environmental education curriculum development processes by using camera. The research, through its epistemological and ontological position, is closely linked to educational orientations associated with aspects of outcomes-based educational transformation in South Africa. A participatory approach that recognises teachers as co-researchers, each bringing to the inquiry her or his questions and constructions of meaning, was adopted. Participatory inquiry was initiated by setting up cluster meetings that allowed for teacher inputs through open dialogic processes. Fundamental to the inquiry is the notion that context shapes curriculum and curriculum development processes. It was from this orientation that a group of 13 teachers, using cameras to create visual narratives, explored their own diverse and complex contexts. These narratives (or stories) form the basis for further inquiry and development of sophistication with respect to the concept of environment. The research process is critically and reflexively documented as a series of field and research texts constructed from a variety of data sources gathered over the period of one year. The work is presented as a process of engaging critically with environmental education curriculum development and an opportunity to raise questions, rather than seek answers in this regard.
- Full Text:
- Date Issued: 1999
A real time Fast Fourier Transform analyser
- Authors: Fisher, John Stanley
- Date: 1980
- Subjects: Fourier transformations Ionosondes Algorithms Computer simulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5439 , http://hdl.handle.net/10962/d1001992
- Description: From the requirements of the Ionosonde digitisation project, undertaken by Rhodes University Antarctic Research Group, it was decided to use the Fast Fourier Transform to compute the spectrum analysis. Several FFT algorithms are reviewed and properties discussed, and the Ccoley Tukey algorithm chosen for utilization. The hardware implementation of this algorithm, and the microprogram control of the whole system are discussed in detail, and such design aspects that required computer simulation are also treated in detail. The final testing of the analyser is shown, and includes a test using data from an ionosonde sounding. The conclusions contain details of extensions to the analysers present operation, required by plans to place the whole Chirpsounder under microprocessor control
- Full Text:
- Date Issued: 1980
- Authors: Fisher, John Stanley
- Date: 1980
- Subjects: Fourier transformations Ionosondes Algorithms Computer simulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5439 , http://hdl.handle.net/10962/d1001992
- Description: From the requirements of the Ionosonde digitisation project, undertaken by Rhodes University Antarctic Research Group, it was decided to use the Fast Fourier Transform to compute the spectrum analysis. Several FFT algorithms are reviewed and properties discussed, and the Ccoley Tukey algorithm chosen for utilization. The hardware implementation of this algorithm, and the microprogram control of the whole system are discussed in detail, and such design aspects that required computer simulation are also treated in detail. The final testing of the analyser is shown, and includes a test using data from an ionosonde sounding. The conclusions contain details of extensions to the analysers present operation, required by plans to place the whole Chirpsounder under microprocessor control
- Full Text:
- Date Issued: 1980
The geology of a portion of south-western Albany
- Meyer, W
- Authors: Meyer, W
- Date: 1965
- Subjects: Geology -- South Africa -- Eastern Cape , Silcrete , Geology, Stratigraphic
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5065 , http://hdl.handle.net/10962/d1013371
- Description: During 1963 an area was mapped around Sidbury, 23 miles south- west of Grahamstown. The object of the survey was to examine stratigraphic problems, which included the nature of a large occurrence of shale south of Alicedale in a region previously mapped as Witteberg, and the relationship between Silcrete and Calcrete. During the invest igation evidence of the existence of two, possibly three , major thrust-faults and of extensive overfolding to the south was discovered. There is reason to believe, that movement on the Zuurberg Fault was initiated in pre-Cretaceous times, and renewed in the early Cretaceous. The Silcrete is shown to be related to the pattern of presentday drainage. Stone implements found embedded in the Calcrete suggest that it is of Recent age.
- Full Text:
- Date Issued: 1965
- Authors: Meyer, W
- Date: 1965
- Subjects: Geology -- South Africa -- Eastern Cape , Silcrete , Geology, Stratigraphic
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5065 , http://hdl.handle.net/10962/d1013371
- Description: During 1963 an area was mapped around Sidbury, 23 miles south- west of Grahamstown. The object of the survey was to examine stratigraphic problems, which included the nature of a large occurrence of shale south of Alicedale in a region previously mapped as Witteberg, and the relationship between Silcrete and Calcrete. During the invest igation evidence of the existence of two, possibly three , major thrust-faults and of extensive overfolding to the south was discovered. There is reason to believe, that movement on the Zuurberg Fault was initiated in pre-Cretaceous times, and renewed in the early Cretaceous. The Silcrete is shown to be related to the pattern of presentday drainage. Stone implements found embedded in the Calcrete suggest that it is of Recent age.
- Full Text:
- Date Issued: 1965
Environmental influences on the daytime vertical distribution of Cape hakes and implications for demersal trawl estimates of hake abundance off the west coast of South Africa
- Authors: Maree, R C
- Date: 2000
- Subjects: Hake , Fisheries -- Environmental aspects -- South Africa , Fisheries -- South Africa , Trawls and trawling
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5278 , http://hdl.handle.net/10962/d1005122
- Description: The demersal fishery off the west coast of South Africa experiences decreased catches per unit effort of hake, Merluccius capensis and M. paradoxus, following the onset of strong south easterly winds. Research has demonstrated that, during daylight hours, Cape hakes migrate vertically in the water column in response to strong south easterly winds, decreasing their availability to the bottom trawl. Hydroacoustic, trawl and environmental data were collected off the West Coast during both calm and wind-swept periods in an attempt to understand the forces that initiate this behaviour, its spatial variability and the implications for demersal trawl estimates of abundance. Near-bottom currents appear to be the primary factor influencing the vertical distribution of the demersal fish community, of which hake constitutes a large proportion, during daylight hours. Correlation between wind and near-bottom currents suggest that the poleward component of the currents increase in velocity within eight hours following the onset of south easterly winds. The fish avoid boundary layers where currents change direction and speed dramatically, and seem to concentrate in waters with relatively stable current regimes. This result questions the assumption of CPUE-based assessment models that hake availability to the bottom trawl is constant or varies randomly. The incorporation of acoustic assessment techniques to demersal surveys has potential, but may be most valuable by supplementing swept-area estimates of abundance, since the sampling efficiency of these methods varies within the water column. The incorporation of wind indices and gear performance data to Cape hake assessment models have been identified as useful considerations for the future.
- Full Text:
- Date Issued: 2000
- Authors: Maree, R C
- Date: 2000
- Subjects: Hake , Fisheries -- Environmental aspects -- South Africa , Fisheries -- South Africa , Trawls and trawling
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5278 , http://hdl.handle.net/10962/d1005122
- Description: The demersal fishery off the west coast of South Africa experiences decreased catches per unit effort of hake, Merluccius capensis and M. paradoxus, following the onset of strong south easterly winds. Research has demonstrated that, during daylight hours, Cape hakes migrate vertically in the water column in response to strong south easterly winds, decreasing their availability to the bottom trawl. Hydroacoustic, trawl and environmental data were collected off the West Coast during both calm and wind-swept periods in an attempt to understand the forces that initiate this behaviour, its spatial variability and the implications for demersal trawl estimates of abundance. Near-bottom currents appear to be the primary factor influencing the vertical distribution of the demersal fish community, of which hake constitutes a large proportion, during daylight hours. Correlation between wind and near-bottom currents suggest that the poleward component of the currents increase in velocity within eight hours following the onset of south easterly winds. The fish avoid boundary layers where currents change direction and speed dramatically, and seem to concentrate in waters with relatively stable current regimes. This result questions the assumption of CPUE-based assessment models that hake availability to the bottom trawl is constant or varies randomly. The incorporation of acoustic assessment techniques to demersal surveys has potential, but may be most valuable by supplementing swept-area estimates of abundance, since the sampling efficiency of these methods varies within the water column. The incorporation of wind indices and gear performance data to Cape hake assessment models have been identified as useful considerations for the future.
- Full Text:
- Date Issued: 2000
Personal autonomy : philosophy and literature
- Authors: Vice, Samantha Wynne
- Date: 1999
- Subjects: Autonomy (Philosophy) , Ishiguro, Kazuo, 1954- -- Remains Of The Day , James, Henry, 1843-1916. Portrait of a lady
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2723 , http://hdl.handle.net/10962/d1002853 , Autonomy (Philosophy) , Ishiguro, Kazuo, 1954- -- Remains Of The Day , James, Henry, 1843-1916. Portrait of a lady
- Description: Gerald Dworkin's influential account of Personal Autonomy offers the following two conditions for autonomy: (i) Authenticity - the condition that one identify with one's beliefs, desires and values after a process of critical reflection, and (ii) Procedural Independence - the identification in (i) must not be "influenced in ways which make the process of identification in some way alien to the individual" (Dworkin 1989:61). I argue in this thesis that there are cases which fulfil both of Dworkin's conditions, yet are clearly not cases of autonomy. Specifically, I argue that we can best assess the adequacy of Dworkin's account of autonomy through literature, because it provides a unique medium for testing his account on the very terms he sets up for himself - ie. that autonomy apply to, and make sense of, persons leading lives of a certain quality. The examination of two novels - Kazuo Ishiguro's The Remains of the Day and Henry James's The Portrait of a Lady - shows that Dworkin's explanation of identification and critical reflection is inadequate for capturing their role in autonomy and that he does not pay enough attention to the role of external factors in preventing or supporting autonomy. As an alternative, I offer the following two conditions for autonomy: (i) critical reflection of a certain kind - radical reflection, and (ii) the ability to translate the results of (i) into action - competence. The novels demonstrate that both conditions are dependent upon considerations of the content of one's beliefs, desires, values etc. Certain of these will prevent or hinder the achievement of autonomy because of their content, so autonomy must be understood in relation to substantial considerations, rather than in purely formal terms, as Dworkin argues.
- Full Text:
- Date Issued: 1999
- Authors: Vice, Samantha Wynne
- Date: 1999
- Subjects: Autonomy (Philosophy) , Ishiguro, Kazuo, 1954- -- Remains Of The Day , James, Henry, 1843-1916. Portrait of a lady
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2723 , http://hdl.handle.net/10962/d1002853 , Autonomy (Philosophy) , Ishiguro, Kazuo, 1954- -- Remains Of The Day , James, Henry, 1843-1916. Portrait of a lady
- Description: Gerald Dworkin's influential account of Personal Autonomy offers the following two conditions for autonomy: (i) Authenticity - the condition that one identify with one's beliefs, desires and values after a process of critical reflection, and (ii) Procedural Independence - the identification in (i) must not be "influenced in ways which make the process of identification in some way alien to the individual" (Dworkin 1989:61). I argue in this thesis that there are cases which fulfil both of Dworkin's conditions, yet are clearly not cases of autonomy. Specifically, I argue that we can best assess the adequacy of Dworkin's account of autonomy through literature, because it provides a unique medium for testing his account on the very terms he sets up for himself - ie. that autonomy apply to, and make sense of, persons leading lives of a certain quality. The examination of two novels - Kazuo Ishiguro's The Remains of the Day and Henry James's The Portrait of a Lady - shows that Dworkin's explanation of identification and critical reflection is inadequate for capturing their role in autonomy and that he does not pay enough attention to the role of external factors in preventing or supporting autonomy. As an alternative, I offer the following two conditions for autonomy: (i) critical reflection of a certain kind - radical reflection, and (ii) the ability to translate the results of (i) into action - competence. The novels demonstrate that both conditions are dependent upon considerations of the content of one's beliefs, desires, values etc. Certain of these will prevent or hinder the achievement of autonomy because of their content, so autonomy must be understood in relation to substantial considerations, rather than in purely formal terms, as Dworkin argues.
- Full Text:
- Date Issued: 1999
An Assessment of Teaching and Learning Strategy in Eastern Selected School, Butterworth District
- Mantanga- Mgoduka, Ntombomzi Beauty
- Authors: Mantanga- Mgoduka, Ntombomzi Beauty
- Date: 2019
- Subjects: School improvement programs -- South Africa -- Eastern Cape High school teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/12447 , vital:39264
- Description: This is a study that was conducted at Nomaheya Senior Secondary School in the Butterworth in East of the Eastern Cape Province. The main purpose of this study was to assesses teaching and learning strategies in improving Grade 12 results. Teaching and Learning Strategies are defined as activities and methods used to move the learner towards achievement of the learning outcome. Crawford, Alan; 2005:16 views teaching and learning strategy, as an effective instruction, as an interactive process - students learn best when they are engaged, not only with their teacher, but also with one another. The researcher will provide the background and motivation of the study. The problem statement, research questions, research objectives, significance of the study, delimitations of the study, literature review, research methodology, and the conclusion of the study. Poor performance that is displayed by Grade 12 learners in the Eastern Cape has been a trend for the past three years. Well known contributory factor for poor performance is poverty, unemployment, high levels of illiteracy, low levels of management and leadership in schools, constant shortage of teachers with specific reference to critical subjects, learner teacher support material (LTSM) that is not delivered on time school safety, infrastructure, discipline amongst learners, absenteeism of learners, SMT with no well-defined roles, Annual Teaching plans, circulars are available but are not implemented. In other words, lack of support by the stakeholders, management and leadership in schools. This was observed by Solo, 1997: 37 who argues that, the atmosphere at home helps to foster or break school functionality, effects of parental interest in learner academic achievement. The background of the study, the problem statement, objectives, questions and also the objectives of research were fully discussed in chapter one. In chapter two, a research on recent publications in the subject was done. Keywords were: outcomebased education, educator, change, assessment and evaluation. The literature study was done to attain objectives in chapter one and also to provide the theoretical background to evaluate the findings in chapter four. vii Chapter three dealt presents the research methodology, research design, area of study, population, sampling method, purposive sampling method, research sample, data collection procedures, data collection instruments, interviews, observations, ethical considerations.
- Full Text:
- Date Issued: 2019
- Authors: Mantanga- Mgoduka, Ntombomzi Beauty
- Date: 2019
- Subjects: School improvement programs -- South Africa -- Eastern Cape High school teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/12447 , vital:39264
- Description: This is a study that was conducted at Nomaheya Senior Secondary School in the Butterworth in East of the Eastern Cape Province. The main purpose of this study was to assesses teaching and learning strategies in improving Grade 12 results. Teaching and Learning Strategies are defined as activities and methods used to move the learner towards achievement of the learning outcome. Crawford, Alan; 2005:16 views teaching and learning strategy, as an effective instruction, as an interactive process - students learn best when they are engaged, not only with their teacher, but also with one another. The researcher will provide the background and motivation of the study. The problem statement, research questions, research objectives, significance of the study, delimitations of the study, literature review, research methodology, and the conclusion of the study. Poor performance that is displayed by Grade 12 learners in the Eastern Cape has been a trend for the past three years. Well known contributory factor for poor performance is poverty, unemployment, high levels of illiteracy, low levels of management and leadership in schools, constant shortage of teachers with specific reference to critical subjects, learner teacher support material (LTSM) that is not delivered on time school safety, infrastructure, discipline amongst learners, absenteeism of learners, SMT with no well-defined roles, Annual Teaching plans, circulars are available but are not implemented. In other words, lack of support by the stakeholders, management and leadership in schools. This was observed by Solo, 1997: 37 who argues that, the atmosphere at home helps to foster or break school functionality, effects of parental interest in learner academic achievement. The background of the study, the problem statement, objectives, questions and also the objectives of research were fully discussed in chapter one. In chapter two, a research on recent publications in the subject was done. Keywords were: outcomebased education, educator, change, assessment and evaluation. The literature study was done to attain objectives in chapter one and also to provide the theoretical background to evaluate the findings in chapter four. vii Chapter three dealt presents the research methodology, research design, area of study, population, sampling method, purposive sampling method, research sample, data collection procedures, data collection instruments, interviews, observations, ethical considerations.
- Full Text:
- Date Issued: 2019
Theory in interpretive psychology - with special reference to Paul Ricoeur's interpretation of Freud
- Authors: Du Toit, Barry
- Date: 1988
- Subjects: Freud, Sigmund, 1856-1939 , Ricœur, Paul , Psychoanalysis
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2906 , http://hdl.handle.net/10962/d1002071
- Description: The thesis aims to show that, while an interpretive psychology is not compatible with theory as it occurs in the predictive- causal explanation of the natural sciences, it is both possible and necessary to develop a concept of theory valid within an interpretive methodology. These claims are advanced in the course of an examination of Ricoeur 's interpretation of Freudian psychoanalysis. After examining some traditional ways in which phenomenological psychology has responded to the psychoanalytic challenge, the thesis presents an interpretation of Freudian psychoanalysis as a hermeneutic approach which utilized theoretical constructions in a productive way, although distorted by Freud's natural-scientific self- understanding. Freud's causal-explanatory language and natural- scientific meta theory are shown to be significant inasmuch as they provide a vehicle for theory construction in psychoanalysis. However, since the theory is modeled on that of the natural sciences, it proves incompatible with the interpretive aspects of Freud's approach. We then establish a concept of theory and of causal analysis which is different to that of the natural sciences, and is compatible with, and indeed founded in, an interpretive approach to psychology. These concepts are then illustrated in the context of psychoanalysis. In the final chapter the advantages of the use of theory in interpretive psychology are discussed.
- Full Text:
- Date Issued: 1988
Theory in interpretive psychology - with special reference to Paul Ricoeur's interpretation of Freud
- Authors: Du Toit, Barry
- Date: 1988
- Subjects: Freud, Sigmund, 1856-1939 , Ricœur, Paul , Psychoanalysis
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: vital:2906 , http://hdl.handle.net/10962/d1002071
- Description: The thesis aims to show that, while an interpretive psychology is not compatible with theory as it occurs in the predictive- causal explanation of the natural sciences, it is both possible and necessary to develop a concept of theory valid within an interpretive methodology. These claims are advanced in the course of an examination of Ricoeur 's interpretation of Freudian psychoanalysis. After examining some traditional ways in which phenomenological psychology has responded to the psychoanalytic challenge, the thesis presents an interpretation of Freudian psychoanalysis as a hermeneutic approach which utilized theoretical constructions in a productive way, although distorted by Freud's natural-scientific self- understanding. Freud's causal-explanatory language and natural- scientific meta theory are shown to be significant inasmuch as they provide a vehicle for theory construction in psychoanalysis. However, since the theory is modeled on that of the natural sciences, it proves incompatible with the interpretive aspects of Freud's approach. We then establish a concept of theory and of causal analysis which is different to that of the natural sciences, and is compatible with, and indeed founded in, an interpretive approach to psychology. These concepts are then illustrated in the context of psychoanalysis. In the final chapter the advantages of the use of theory in interpretive psychology are discussed.
- Full Text:
- Date Issued: 1988