Parent characterization of quality protein maize (Zea mays L.) and combining ability for tolerance to drought stress
- Authors: Pfunde, Cleopatra Nyaradzo
- Date: 2012
- Subjects: Corn -- Quality , Corn as food , Corn -- Effect of stress on , Corn -- Effect of drought on , Cluster analysis , Crops -- Effect of drought on , Corn -- Drought tolerance , Corn -- Breeding , Crops -- Drought tolerance
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11869 , http://hdl.handle.net/10353/d1007536 , Corn -- Quality , Corn as food , Corn -- Effect of stress on , Corn -- Effect of drought on , Cluster analysis , Crops -- Effect of drought on , Corn -- Drought tolerance , Corn -- Breeding , Crops -- Drought tolerance
- Description: Quality protein maize (QPM) has enhanced levels of two essential amino acids, lysine and tryptophan compared to normal maize. This makes QPM an important cereal crop in communities where maize is a staple crop. The main abiotic factor to QPM production is drought stress. Little information is available on the effect of drought stress on QPM. Therefore, the objectives of this study were to: (i) conduct diversity analysis of QPM inbred lines using morpho-agronomic and simple sequence repeat markers, (ii) screen available QPM inbred lines and F1 progeny for tolerance to seedling drought stress, (iii) determine the combining ability and type of gene action of QPM inbred lines for tolerance to seedling drought stress, grain yield and endosperm modification. The study was conducted in South Africa, at the University of Fort Hare. Morphological characterisation of 21 inbred lines was done using quantitative and qualitative traits. A randomised complete block design with three replicates was used for characterizing the inbred lines in the field. Genstat statistical software, version 12 (Genstat ®, 2009) was used for analysis of variance (ANOVA) and descriptive statistics. Analysis of variance was performed on all quantitative data for morphological traits. Data for qualitative traits was tabulated in their nominal classes. Traits that contributed most to the variation were days to anthesis, days to silking, anthesis-silking interval, plant height, number of kernel rows, ear length and grain yield. Cluster analysis grouped the inbred lines into three main clusters. The first cluster was characterised by tall and average yielding lines, while the second cluster showed the least anthesis-silking interval, and had the highest yield. Cluster three consisted of lines that were early maturing, but were the least yielding. Genetic distances between maize inbred lines were quantified by using 27 simple sequence repeat markers. The genetic distances between genotypes was computed using Roger’s (1972) genetic distances. Cluster analysis was then carried out using the neighbour-joining tree method using Power Marker software version 3.25. A dendrogram generated from the genetic study of the inbred lines revealed three groups that concurred with expectations based upon pedigree data. These groups were not identical to the groups generated using morpho-agronomic characterisation. Twenty one QPM inbred lines were crossed using a North Carolina design II mating scheme. These were divided into seven sets, each with three inbred lines. The three inbred lines in one set were used as females and crossed with three inbred lines in another set consisting of males. Each inbred line was used as a female in one set, and as a male in a second set. Sixty three hybrids (7 sets x 9 hybrids) were formed and evaluated in October 2011, using a 6x8 alpha-lattice incomplete block design with three replicates under glasshouse and optimum field conditions. A randomised complete block design with three replicates was used for the 21 parental inbred lines. Traits recorded for the glasshouse study were, canopy temperature, chlorophyll content, leaf roll, stem diameter, plant height, leaf number, leaf area, fresh and dry root and shoot weights. Data for the various traits for each environment, 25 percent (stress treatment) and 75 percent (non-stress) of field capacity, were subjected to analysis of variance using the unbalanced treatment design in Genstat statistical package Edition 12. Where varietal differences were found, means were separated using Tukey’s test. Genetic analyses for grain yield and agronomic traits were performed using a fixed effects model in JMP 10 following Residual Maximum Likelihood procedure (REML). From the results, inbred lines that were not previously classified into heterotic groups and drought tolerance categories were classified based on their total dry weight performance and drought susceptibility index. Inbred lines L18, L9, L8, L6 and L3, in order of their drought tolerance index were the best performers under greenhouse conditions and could be recommended for breeding new varieties that are tolerant to seedling drought stress. Evaluation of maize seedlings tolerant to drought stress under glasshouse conditions revealed that cross combination L18 x L11 was drought tolerant, while cross L20 x L7 was susceptible. Total dry weight was used as the major criteria for classifying F1 maize seedlings as being resistant or susceptible. General combining ability effects accounted for 67.43 percent of the genetic variation for total dry weight, while specific combining ability effects contributed 37.57 percent. This indicated that additive gene effects were more important than non-additive gene action in controlling this trait. In the field study (non-drought), the experimental design was a 6x8 alpha lattice incomplete block design with three replicates. On an adjacent field a randomised complete block design with three replicates was used to evaluate the parental inbred lines. The following variables were recorded: plant height, ear height, ears per plant, endosperm modification, days to silking and days to anthesis, anthesis-silking interval, number of kernels per row, number of rows per ear and grain yield. General analyses for the incomplete lattice block design and randomised complete block design for hybrid and inbred data respectively were performed using JMP 10 statistical software. Means were separated using the Tukey's test. Genetic analyses of data for grain yield and agronomic traits were conducted using a fixed effects model using REML in JMP 10. The importance of both GCA (51 percent) and SCA (49 percent) was observed for grain yield. A preponderance of GCA existed for ear height, days to anthesis, anthesis-silking interval, ears per plant and number of kernels per row, indicating that predominantly, additive gene effects controlled hybrid performance under optimum field conditions. The highest heritability was observed for days to silking (48.27 percent) suggesting that yield could be improved through selection for this trait. Under field conditions, variation in time to maturity was observed. This implies that these inbred lines can be recommended for utilisation in different agro-ecologies. Early maturing lines such as L18 can be used to introduce earliness in local cultivars, while early maturing single crosses such as L18 x L2, L5 x L9, L3 x L4 and L2 x L21 could be recommended for maize growers in drought prone areas such as the former Ciskei. Single crosses L18xL11, L16xL18, L8xL21 and L9xL6 had good tolerance to seedling drought stress. On the other hand, single crosses L18xL11 and L11xL13 had high grain yield and good endosperm modification. All these single crosses could be recommended for commercial production after evaluation across locations in the Eastern Cape Province. Alternatively they can be crossed with other superior inbreds to generate three or four way hybrids, which could then be evaluated for potential use by farmers in the Eastern Cape.
- Full Text:
- Authors: Pfunde, Cleopatra Nyaradzo
- Date: 2012
- Subjects: Corn -- Quality , Corn as food , Corn -- Effect of stress on , Corn -- Effect of drought on , Cluster analysis , Crops -- Effect of drought on , Corn -- Drought tolerance , Corn -- Breeding , Crops -- Drought tolerance
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11869 , http://hdl.handle.net/10353/d1007536 , Corn -- Quality , Corn as food , Corn -- Effect of stress on , Corn -- Effect of drought on , Cluster analysis , Crops -- Effect of drought on , Corn -- Drought tolerance , Corn -- Breeding , Crops -- Drought tolerance
- Description: Quality protein maize (QPM) has enhanced levels of two essential amino acids, lysine and tryptophan compared to normal maize. This makes QPM an important cereal crop in communities where maize is a staple crop. The main abiotic factor to QPM production is drought stress. Little information is available on the effect of drought stress on QPM. Therefore, the objectives of this study were to: (i) conduct diversity analysis of QPM inbred lines using morpho-agronomic and simple sequence repeat markers, (ii) screen available QPM inbred lines and F1 progeny for tolerance to seedling drought stress, (iii) determine the combining ability and type of gene action of QPM inbred lines for tolerance to seedling drought stress, grain yield and endosperm modification. The study was conducted in South Africa, at the University of Fort Hare. Morphological characterisation of 21 inbred lines was done using quantitative and qualitative traits. A randomised complete block design with three replicates was used for characterizing the inbred lines in the field. Genstat statistical software, version 12 (Genstat ®, 2009) was used for analysis of variance (ANOVA) and descriptive statistics. Analysis of variance was performed on all quantitative data for morphological traits. Data for qualitative traits was tabulated in their nominal classes. Traits that contributed most to the variation were days to anthesis, days to silking, anthesis-silking interval, plant height, number of kernel rows, ear length and grain yield. Cluster analysis grouped the inbred lines into three main clusters. The first cluster was characterised by tall and average yielding lines, while the second cluster showed the least anthesis-silking interval, and had the highest yield. Cluster three consisted of lines that were early maturing, but were the least yielding. Genetic distances between maize inbred lines were quantified by using 27 simple sequence repeat markers. The genetic distances between genotypes was computed using Roger’s (1972) genetic distances. Cluster analysis was then carried out using the neighbour-joining tree method using Power Marker software version 3.25. A dendrogram generated from the genetic study of the inbred lines revealed three groups that concurred with expectations based upon pedigree data. These groups were not identical to the groups generated using morpho-agronomic characterisation. Twenty one QPM inbred lines were crossed using a North Carolina design II mating scheme. These were divided into seven sets, each with three inbred lines. The three inbred lines in one set were used as females and crossed with three inbred lines in another set consisting of males. Each inbred line was used as a female in one set, and as a male in a second set. Sixty three hybrids (7 sets x 9 hybrids) were formed and evaluated in October 2011, using a 6x8 alpha-lattice incomplete block design with three replicates under glasshouse and optimum field conditions. A randomised complete block design with three replicates was used for the 21 parental inbred lines. Traits recorded for the glasshouse study were, canopy temperature, chlorophyll content, leaf roll, stem diameter, plant height, leaf number, leaf area, fresh and dry root and shoot weights. Data for the various traits for each environment, 25 percent (stress treatment) and 75 percent (non-stress) of field capacity, were subjected to analysis of variance using the unbalanced treatment design in Genstat statistical package Edition 12. Where varietal differences were found, means were separated using Tukey’s test. Genetic analyses for grain yield and agronomic traits were performed using a fixed effects model in JMP 10 following Residual Maximum Likelihood procedure (REML). From the results, inbred lines that were not previously classified into heterotic groups and drought tolerance categories were classified based on their total dry weight performance and drought susceptibility index. Inbred lines L18, L9, L8, L6 and L3, in order of their drought tolerance index were the best performers under greenhouse conditions and could be recommended for breeding new varieties that are tolerant to seedling drought stress. Evaluation of maize seedlings tolerant to drought stress under glasshouse conditions revealed that cross combination L18 x L11 was drought tolerant, while cross L20 x L7 was susceptible. Total dry weight was used as the major criteria for classifying F1 maize seedlings as being resistant or susceptible. General combining ability effects accounted for 67.43 percent of the genetic variation for total dry weight, while specific combining ability effects contributed 37.57 percent. This indicated that additive gene effects were more important than non-additive gene action in controlling this trait. In the field study (non-drought), the experimental design was a 6x8 alpha lattice incomplete block design with three replicates. On an adjacent field a randomised complete block design with three replicates was used to evaluate the parental inbred lines. The following variables were recorded: plant height, ear height, ears per plant, endosperm modification, days to silking and days to anthesis, anthesis-silking interval, number of kernels per row, number of rows per ear and grain yield. General analyses for the incomplete lattice block design and randomised complete block design for hybrid and inbred data respectively were performed using JMP 10 statistical software. Means were separated using the Tukey's test. Genetic analyses of data for grain yield and agronomic traits were conducted using a fixed effects model using REML in JMP 10. The importance of both GCA (51 percent) and SCA (49 percent) was observed for grain yield. A preponderance of GCA existed for ear height, days to anthesis, anthesis-silking interval, ears per plant and number of kernels per row, indicating that predominantly, additive gene effects controlled hybrid performance under optimum field conditions. The highest heritability was observed for days to silking (48.27 percent) suggesting that yield could be improved through selection for this trait. Under field conditions, variation in time to maturity was observed. This implies that these inbred lines can be recommended for utilisation in different agro-ecologies. Early maturing lines such as L18 can be used to introduce earliness in local cultivars, while early maturing single crosses such as L18 x L2, L5 x L9, L3 x L4 and L2 x L21 could be recommended for maize growers in drought prone areas such as the former Ciskei. Single crosses L18xL11, L16xL18, L8xL21 and L9xL6 had good tolerance to seedling drought stress. On the other hand, single crosses L18xL11 and L11xL13 had high grain yield and good endosperm modification. All these single crosses could be recommended for commercial production after evaluation across locations in the Eastern Cape Province. Alternatively they can be crossed with other superior inbreds to generate three or four way hybrids, which could then be evaluated for potential use by farmers in the Eastern Cape.
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Winter rotational cover crops effects on soil strength, aggregate stability and water conservation of a hardsetting cambisol in Eastern Cape Province, South Africa
- Authors: Mupambwa, Hupenyu Allan
- Date: 2012
- Subjects: Cover crops -- South Africa -- Eastern Cape , Soil mechanics , Crop rotation , Crops and soils , Soil penetration test
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11868 , http://hdl.handle.net/10353/453 , Cover crops -- South Africa -- Eastern Cape , Soil mechanics , Crop rotation , Crops and soils , Soil penetration test
- Description: Winter rotational cover crops (WRCC) are often used to boost soil fertility and plant nutrition. However, selection and use of WRCC for soil physical improvement is usually overlooked. The objective of this study was to determine the effects of WRCC on soil strength, aggregate stability and water conservation of a hardsetting soil. The soil physical properties were determined after four rotations of growing monocultures of vetch (Vicia dasycarpa cv. Max), lupin (Lupinus angustifolius cv. Tanjil) and oats (Avena sativa cv. Sederberg) and after two rotations of growing bicultures of oats (Avena sativa cv. Pallinup) and vetch (Vicia dasycarpa cv. Max) across two soil layers, 0 to 15 cm and 15 to 30 cm. The individual WRCC and a weedy fallow constituted the treatments in the monoculture study whilst in the biculture study the various combinations of WRCC namely; 90% oat plus 10% vetch (O90V10); 70% oat plus 30% vetch (O70V30) and 50% oat plus 50% vetch (O50V50) and a weedy fallow constituted the treatments. After four rotations with cover crop monocultures, oats significantly (P ≤ 0.05) reduced penetration resistance (PR) whilst vetch increased PR in both soil layers compared to the weedy fallow control. The effect of the biculture treatments was only experienced within the 15 to 30 cm depth. The treatments O50V50 and O70V30 increased the PR compared to the control. The WRCC in monoculture significantly increased the soil aggregate stability relative to the control in both soil layers. Vetch, lupin and oats resulted in a 41.7%; 20.4% and 15.7% increase in MWD in the 0 to 15 cm soil layer and 47.2%; 44.2% and 39.7% in the 15 to 30 cm depth, respectively. An increase in aggregate stability was associated with increased macro-aggregation. Under the biculture, WRCC slightly increased, non- significantly, the aggregate stability. Both hot water and dilute acid extractable polysaccharides showed no significant correlation with aggregate stability in the two studies. Oats monoculture resulted in a significant difference (P ≤ 0.05) on cumulative infiltration compared to the control. However, after 2 h vetch and lupin showed no significant difference from the control on cumulative infiltration. Oats resulted in a 7.8% increase in final infiltration rate (FIR) whilst vetch and lupin reduced FIR by 9% and 16.7% respectively, compared to the control. Bicultures of oats and vetch significantly (P ≤ 0.05) increased cumulative infiltration compared to the weedy fallow control. A similar significant increase in FIR was also observed under bicultures. The treatments O50V50; O90V10 and O70V30 resulted in a 163.3%; 113.3% and 105.4% increase in FIR respectively, compared to the control. Cover crop monocultures significantly (P ≤ 0.05) increased plant available water (PAW) compared to the weedy fallow, with vetch, oats and lupin resulting in a 28.3%; 22% and 23.9% increase respectively, in PAW. However, no significant differences were observed on PAW after two rotations with bicultures. Compared with winter weedy fallow, WRCC improved most of the soil physical properties under study, with the most suitable results expected under bicultures compared to monocultures. Under CA, selection of WRCC like oats, vetch and lupin, one should therefore take into consideration their effects on soil physical properties as a selection criterion and not biomass and fertility alone.
- Full Text:
- Authors: Mupambwa, Hupenyu Allan
- Date: 2012
- Subjects: Cover crops -- South Africa -- Eastern Cape , Soil mechanics , Crop rotation , Crops and soils , Soil penetration test
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11868 , http://hdl.handle.net/10353/453 , Cover crops -- South Africa -- Eastern Cape , Soil mechanics , Crop rotation , Crops and soils , Soil penetration test
- Description: Winter rotational cover crops (WRCC) are often used to boost soil fertility and plant nutrition. However, selection and use of WRCC for soil physical improvement is usually overlooked. The objective of this study was to determine the effects of WRCC on soil strength, aggregate stability and water conservation of a hardsetting soil. The soil physical properties were determined after four rotations of growing monocultures of vetch (Vicia dasycarpa cv. Max), lupin (Lupinus angustifolius cv. Tanjil) and oats (Avena sativa cv. Sederberg) and after two rotations of growing bicultures of oats (Avena sativa cv. Pallinup) and vetch (Vicia dasycarpa cv. Max) across two soil layers, 0 to 15 cm and 15 to 30 cm. The individual WRCC and a weedy fallow constituted the treatments in the monoculture study whilst in the biculture study the various combinations of WRCC namely; 90% oat plus 10% vetch (O90V10); 70% oat plus 30% vetch (O70V30) and 50% oat plus 50% vetch (O50V50) and a weedy fallow constituted the treatments. After four rotations with cover crop monocultures, oats significantly (P ≤ 0.05) reduced penetration resistance (PR) whilst vetch increased PR in both soil layers compared to the weedy fallow control. The effect of the biculture treatments was only experienced within the 15 to 30 cm depth. The treatments O50V50 and O70V30 increased the PR compared to the control. The WRCC in monoculture significantly increased the soil aggregate stability relative to the control in both soil layers. Vetch, lupin and oats resulted in a 41.7%; 20.4% and 15.7% increase in MWD in the 0 to 15 cm soil layer and 47.2%; 44.2% and 39.7% in the 15 to 30 cm depth, respectively. An increase in aggregate stability was associated with increased macro-aggregation. Under the biculture, WRCC slightly increased, non- significantly, the aggregate stability. Both hot water and dilute acid extractable polysaccharides showed no significant correlation with aggregate stability in the two studies. Oats monoculture resulted in a significant difference (P ≤ 0.05) on cumulative infiltration compared to the control. However, after 2 h vetch and lupin showed no significant difference from the control on cumulative infiltration. Oats resulted in a 7.8% increase in final infiltration rate (FIR) whilst vetch and lupin reduced FIR by 9% and 16.7% respectively, compared to the control. Bicultures of oats and vetch significantly (P ≤ 0.05) increased cumulative infiltration compared to the weedy fallow control. A similar significant increase in FIR was also observed under bicultures. The treatments O50V50; O90V10 and O70V30 resulted in a 163.3%; 113.3% and 105.4% increase in FIR respectively, compared to the control. Cover crop monocultures significantly (P ≤ 0.05) increased plant available water (PAW) compared to the weedy fallow, with vetch, oats and lupin resulting in a 28.3%; 22% and 23.9% increase respectively, in PAW. However, no significant differences were observed on PAW after two rotations with bicultures. Compared with winter weedy fallow, WRCC improved most of the soil physical properties under study, with the most suitable results expected under bicultures compared to monocultures. Under CA, selection of WRCC like oats, vetch and lupin, one should therefore take into consideration their effects on soil physical properties as a selection criterion and not biomass and fertility alone.
- Full Text:
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