List of Titles

In vitro investigation of the anti-hyperglycemic and chemical composition of Heteromorpha arborescens (Spreng.) Cham leaf extracts used in the management of diabetes mellitus

- Abifarin, Taiwo Oluwafunmilola https://orcid.org/00000003-4994-4638

Authors: Abifarin, Taiwo Oluwafunmilola https://orcid.org/00000003-4994-4638
Date: 2021-09
Subjects: Diabetes
Language: English
Type: Doctoral theses , text
Identifier: http://hdl.handle.net/10353/22798 , vital:52760
Description: Diabetes mellitus is currently a major threat all over the world. It is a chronic disorder characterized by elevated blood glucose levels and disturbance in carbohydrate, fat and protein metabolism which often times leads to several complications such as, atherosclerosis, diabetic nephropathy, retinopathy and neuropathy. Due to the side effects associated with synthetic oral hypoglycemic drugs, herbal remedies have become the alternative in the management of diabetes mellitus. Heteromorpha arborescens is one of the many plants used as herbal remedies for the management of diabetes mellitus in South Africa. In addition, the roots, barks and leaves are used in other parts of Southern Africa for the treatment of mental problems, asthma, cough, dysentery and tuberculosis. The roots are fed to malnourished children in Botswana and Swaziland. However, despite the high medicinal importance, there is little or no scientificinformation to prove this claim. Therefore, the basis of this study is to determine the chemical composition as well as perform and invitro investigation of the antihyperglycemic potential of H. arborescens leaf extracts to provide information that could validate the ethno-medicinal claims for the use of this plant in the management of diabetes mellitus. Phytochemical contents and antioxidant activity of the leaf extracts were determined. Phytochemical analysis of the acetone, ethanol, aqueous and blanched extracts of H. arborescens leaves indicated that the total phenol content of the extracts ranged between 15.10 mg GAE/g- 42.50 mg QAE/g, proanthocyanidin, 459-8402.1 mg QE/g and the flavonoid content, 109.24- 235.79 mg QE/g. In addition, alkaloids (7.65percent) and saponin (25.33percent) were present in significantamounts. Based on the IC50 values, the ethanol extract exhibited the highest total antioxidant activity (0.0125 mg/mL) with highest inhibition against DPPH and ABTS radicals (0.06 and 0.049 mg/mL respectively). Relatively high antioxidant activity may be attributed to the polyphenolic contents which possess hypoglycemic potentials. Minimum Inhibitory Concentrations (MIC) of H. arborescens leaf extracts against Bacillus pumilus, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli and Klebselia pneumoniae was also determined and results indicated considerable antibacterial activity in the acetone, ethanol and blanched extracts with MIC values ranging from 1.563-12.5 mg/mL; however, the aqueous extract was inactive against all the bacteria strains. The antibacterial activity of the leaf extracts could be advantageous against diabetic related infections. The cytotoxicity, anti-obesity and antidiabetic potentials of blanched, aqueous and ethanol extracts of Heteromorpha arborescens (Spreng.) Cham leaves were also investigated. The results revealed that both ethanol and aqueous extracts indicated considerable inhibition against α-glucosidase (IC50 of 627.29 ± 4.33 μg/mL and 576.46 ± 3.21 μg/mL respectively) while the blanched extract showed weak α- glucosidase inhibition (IC50; 855.38 ± 4.29 μg/mL). The aqueous extract showed the best α- amylase inhibition (IC50; 583.74 ± 5.87 μg/mL) among the assessed extracts. However, weak α- amylase inhibition was observed in the ethanol (IC50; 724.60 ± 4.33 μg/mL) and blanched extracts (IC50; 791.63 ± 3.76 μg/mL) of H. arborescens leaves. Some level of glucose utilization in both C3A and L6 cells was also observed for the aqueous and ethanol extracts which may be attributed to the relatively lower toxicity levels present in them, however, glucose utilization was very weak for the blanched extract, which may be due to higher level of cytotoxicity it possessed. Relatively weak lipase inhibition was observed for the ethanol (IC50; 699.3 ± 1.33 μg/mL), aqueous (IC50; 811.52 ± 3.52 μg/mL) and blanched extract (IC50; 1152.7 ± 4.61 μg/mL) as compared to orlistat (IC50; 56.88 ± 0.11 μg/mL). However, there was no reasonable reduction in lipid accumulation observed in all the extract treated cells. The results obtained suggest that H.arborescens leaf extracts can serve as a potential tool for the development of new strategies for the treatment of diabetes and obesity. However, further analysis is required to ascertain its anti- obesity potential. Also, caution should be taken in the use of the plant at high concentrations in order to ensure safety and efficacy. Analyses were also carried out to determine the nutritional and antinutritional constituents of the plant for possible inclusion in the diet of diabetic patients. Proximate analysis revealed the presence of 8.5 percent total ash, 4.92 percent crude fat, 8.41 percent moisture, 15.74 percent crude protein, 21.48 percent crude fiber, 40.95 percent carbohydrates and 271.04 kcal/100g energy value. Mineral analysis showed that H. arborescens leaves are very rich in K, Ca, and Fe. Considerable amounts of Mg, Mn, Na, P, Cu and Zn were also present. Vitamin analysis showed that the plant has a high content of vitamins A, C and E. The anti-nutrients evaluated were phytate, oxalate, saponin, and alkaloids, all of which were below toxic levels except for saponin which was observed in moderately high level. This study revealed that H. arborescens leaves are a good source of nutrients and mineral elements, (with low anti-nutrient content) that are highly beneficial to human health especially in diabetic individuals, therefore, encouraging its possible inclusion as a vegetable. Essential oil composition of fresh Heteromorphaarborescens leaves were also determined by Solvent-Free-Microwave-Extraction (SFME) and Hydrodistillation (HD) methods and the compositions of both mehods were compared in terms of their chemical compositions, yield, CO2 emission and energy consumption. Solvent Free Microwave extraction method indicated higher oil yield of 0.7 mL/200 g (0.35 percent) as compared to 0.59 mL/200 g (0.295 percent), lower energy consumption and CO2 emission as compared to the hydrodistillation method. In conclusion, H. arborescens leaves indicated considerable potential efficacy in the management for diabetes mellitus and may require further structural elucidation and characterization in order to identify the bioactive constituents. , Thesis (PhD) -- Faculty of Science and Agriculture, 2021
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Date Issued: 2021-09

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Acculturation and Coming of age in female African writing; a Freudian psychoanalysis of Tsitsi Dangarembga’s Nervous Conditions and Chimamada Adichie’s Purple Hibiscus

- Abiodun, Adedoyin Catherine

Authors: Abiodun, Adedoyin Catherine
Date: 2019
Subjects: African literature (English)
Language: English
Type: Thesis , Masters , MA
Identifier: http://hdl.handle.net/10353/16274 , vital:40704
Description: This study explores Acculturation and Coming of age not only as a social process but also a psychological one. The constructs are examined in line with Freudian psychoanalytic theory. The study focuses on migrant inclination of two female African writers, Tsitsi Dangarembga and Chimamanda Adichie in Nervous Conditions and Purple Hibiscus respectively. Through the study, it is discovered that acculturation involves both cultural and psychological change or adaptation and failure in either can result in trauma or produce socially imbalanced individuals. In other to have a healthy coming of age, family and the home status play a very significant role in the totality of an individual and also serves as a microcosm of social and political milieu. Also, the study in the course of the study, we discover there is no ‘authentic African culture’, culture is non-static and so, the study also discusses culture as being transnational and translational. The writers’ consciousness of space and place in their writing through reminiscent times of childhood play significant roles. Childhood figures are constructed in a matrix of concrete memories, spaces, places and times that play a significant role in the production of meanings of their migrant identities. The study identifies ways in which female socialisation further enhances her marginalisation in the society and how the family in the African setting as an ideological state apparatus contributes in ensuring the marginalised position of women. The authors being studied interrogate methods of raising children among African families in contemporary society
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Date Issued: 2019

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International legal protection of women's reproductive rights: a comparative analysis of abortion laws and policies in four jurisdictions-Nigeria, Ghana, South Africa and U.S.A.

- Abiodun, Adeleke Funminiyi

Authors: Abiodun, Adeleke Funminiyi
Date: 2010-10
Subjects: Abortion -- Law and legislation , Reproductive right
Language: English
Type: Doctoral theses , text
Identifier: http://hdl.handle.net/10353/25839 , vital:64491
Description: The subject matter of abortion law is extremely broad and multi-disciplinary. While naturally having its basis in criminal and constitutional law of individual states, the global development of abortion discourse has been influenced by the need to protect the reproductive rights of women as a sub-set of international human rights laws; thereby advocating not only the decriminalisation of abortion but also, that individual State should take affirmative actions in promoting abortion as a right for every woman. However, that induced abortion is a punishable criminal act or a “protectable” woman’s reproductive right remains controversial due to the plethora of perspectives, beliefs and reservations held by different groups of people which are multi-dimensional and contradictory. This thesis therefore attempts a comparative study of abortion laws and policies in four jurisdictions: Nigeria and Ghana, operating criminalised abortion laws, South Africa and United States which operate liberalised/permissive abortion laws and policies. The study basically examines the legal status and reproductive rights of women to legal and safe abortion within the existing legal framework of national laws of the four selected jurisdictions vis-à-vis the legal protection offered by various international instruments on human rights. While we show that strict and restrictive abortion laws, and also, failure of States to create conditions for safe abortion constitute infractions of the reproductive rights of women, we submit further that over-liberalisation of abortion laws and policies could also amount to infringement of the basic rights of other people, thus there is need to ensure a legal and acceptable balance. The study finally acknowledges the role of international human rights laws in the protection of women’s right to legal and safe abortion but asserts that there can be no universally acceptable morality to which the whole world could subsume in term of women’s right to abortion due to the interplay of socio-cultural, religious, and moral affiliations of the people in different communities. , Thesis (LLD) -- Faculty of Law, 2010
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Date Issued: 2010-10

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Comparative studies of different ant-hypertensive treatments used for cardiovascular disease patients : a case study of public health facility in the Eastern Cape South Africa

- Abiodun, Oluwatoyin Victoria

Authors: Abiodun, Oluwatoyin Victoria
Date: 2017
Subjects: Survival analysis (Biometry) Cardiovascular system -- Diseases
Language: English
Type: Thesis , Masters , MSc
Identifier: http://hdl.handle.net/10353/13205 , vital:39519
Description: Discovery has shown that many deaths arising from cardiovascular disease often show early symptoms of high blood pressure, which makes it the leading risk factor of cardiovascular diseases. There is a distressing rate of 970 million hypertensive patients worldwide, a higher ratio of 640 million are from developing countries, if proper measures are not taken, there will be a higher growth of cardiovascular disease in adults by 2025. The focus of this study was to compare the therapeutic potentials of the anti-hypertensive therapies, drug A (amiloride + furosemide) and drug B (hydrochlorothiazide + enalapril) used in the treatment of cardiovascular disease patients for their capability to lengthen remission duration and survival time. All patients were diagnosed with a type of cardiovascular disorder, therefore patient records were followed up and blood pressure was being monitored for a period of two years. The retrospective data gotten from the public health facility was analysed using survival analysis methods. The results revealed that there was no statistical significance in the distributions of the length of remission and survival time of drug A and drug B patients. Also, in the age category, CVD patients that are 55 and above recover faster with drug A, while CVD patients below 55 shows a better survival with drug B. Then again, estimates from the gender category show a better survival in males using drug A, while the females thrive slightly better using drug B. Among all the risk factors considered in the study, only employment status showed a strong impact on survival time (remission). The findings from this study may help to formulate interventions and strategies that will increase the lifespan of cardiovascular patients and reduce early mortality due to cardiovascular diseases.
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Date Issued: 2017

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Molecular studies on cholera and non-cholera causing vibrio pathogens isolated from some water resources and aquatic animals in Eastern Cape Province, South Africa

- Abioye, Oluwatayo Emmanuel

Authors: Abioye, Oluwatayo Emmanuel
Date: 2018
Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
Language: English
Type: Thesis , Doctoral , PhD
Identifier: http://hdl.handle.net/10353/9154 , vital:34283
Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
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Date Issued: 2018

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Rural-urban migration and its impact on rural development in Nigeria

- Abizu, Odion Stanley

Authors: Abizu, Odion Stanley
Date: 2018
Subjects: Rural development Rural-urban migration -- Nigeria Rural poor
Language: English
Type: Thesis , Doctoral , DPhil
Identifier: http://hdl.handle.net/10353/13815 , vital:39714
Description: This thesis focuses on the developmental impact of rural-urban migration in Nigeria. While, recognising the negative impact of rural-urban migration, the study argues that in order to benefit internal migrants as well as rural development, there must be a paradigm shift to focus on how the benefits of migration on development can be achieved. It is proposed that rural-urban migration occurs as a response to economic, education, health, social and environmental factors. The challenges confronting rural areas are urban bias development paradigm pursued by the successive governments resulting in rural underdevelopment that exacerbates the rural urban disparity. And the neglect of agriculture in rural areas resulting from the discovery of oil in Nigeria. The study revealed that flawed policies adopted by successive Nigerian governments led to joblessness, entrenched social inequality and opportunities. One of the outcomes of these short-sighted policies was rural underdevelopment, which accelerated rural-urban migration in Nigeria. However, the study also demonstrates that the income flows from the migrants to the various rural households has contributed to improvement in the living standard of the households. The fragmentary approaches to rural development that is currently the case in Nigeria would not generate the required level of development and growth needed in the rural communities to improve the living standard. Thus, there is need for a policy framework in Nigeria, which would encourage the private sector to play a vital role in contributing their part in the provision of infrastructural facilities in the rural communities. Although this thesis is a case study of the impact of rural-urban migration on rural development in Nigeria, it can serve to appreciate the role migration can play in promoting development both in rural and urban areas in Nigeria and other Saharan Africa countries.
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Date Issued: 2018

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Perceptions of social media as a tool for research: a study of postgraduate students, University of Fort Hare, Alice campus

- Abolanle, Ogunnubi Adeyemi

Authors: Abolanle, Ogunnubi Adeyemi
Date: 2014-10
Subjects: University of Fort Hare , Media literacy , Internet research
Language: English
Type: Master's theses , text
Identifier: http://hdl.handle.net/10353/26381 , vital:65299
Description: The study aimed at examining the perceptions of social media as a tool for research among postgraduate students at the University of Fort Hare, Alice campus. Data were collected using a quantitative research approach by administering structured questionnaires to 310 postgraduate students under the cluster sampling across different faculties and departments, and analysed by SPSS Statistical software and Chi-square test. 264 questionnaires were returned for analysis and these respondents maintained that they utilized social media platforms for their academic research. The findings further revealed that social media has to a large extent improved students‟ research activities as they frequently seek practical knowledge for demonstration and tutorship. Furthermore, the respondents regard social media as a good platform for research topics in audio and video formats, scholarly articles, journals and books. This study concluded that since students explore the social media platforms and search engines for various reasons, academic research inclusive, it therefore becomes imperative to create more links within the social media for students to explore books, scholarly journals and articles necessary for research and academic purposes. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2014
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Date Issued: 2014-10

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Prevalence of Escherichia coli O157:H7 in water and meat and meat products and vegetables sold in the Eastern Cape Province of South Africa and its impact on the diarrhoeic conditions of HIV/AIDS patients

- Abong'o, Benard Omondi

Authors: Abong'o, Benard Omondi
Date: 2008
Subjects: Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
Language: English
Type: Thesis , Doctoral , PhD (Microbiology)
Identifier: vital:11263 , http://hdl.handle.net/10353/87 , Foodborne diseases , Diarrhea , Escherichia coli , HIV infections , AIDS (Disease) , Bacterial diseases
Description: Water and food borne Escherichia coli O157:H7 could be one of the pathogens posing high health risk to patients suffering from Acquired Immunodeficiency Syndrome (AIDS) because of its incrimination in diarrhoea cases in AIDS patients. The present study, which was conducted between March 2005 and August 2006, investigated the prevalence of E. coli O157:H7 in water, meat and meat products and vegetables and its impact on diarrhoeic conditions of confirmed and non-confirmed HIV/AIDS patients in the Amathole District in the Eastern Cape Province of South Africa. The water samples used in the study were obtained from stand pipes supplying treated drinking water to communities residing in Fort Beaufort, Alice, Dimbaza and Mdantsane whereas borehole waters were sampled from Ngwenya and Kwasaki. The meat and meat products and vegetable samples were purchased from shops, butcheries, supermarkets and open air markets in Fort Beaufort, Alice and Mdantsane. The stool swabs used in the study were obtained from HIV/AIDS and outpatient clinics at Frere Hospital in East London. A total of 180 each of water, meat and meat products and vegetable samples and another 360 stool samples were analyzed for E. coli O157:H7. Presumptive E. coli O157 was isolated from the samples by culture-based methods and confirmed using Polymerase Chain Reaction techniques. Anti-biogram as well as risk assessment were also carried out using standard methods. The viable counts of presumptive E. coli O157 for water samples ranged between 3.3 × 104 and 1.71 × 105 CFU/ml, and between 1.8 × 104 and 5.04 × 106 CFU/g for meat and meat products, whereas those for vegetables ranged between 1.3 × 103 and 1.6 × 106 CFU/g. The counts of presumptive E. coli O157 for the water and vegetable samples were not significantly different whereas those for meat and meat products were found to be significantly different (P ≤ 0.05). The prevalence rates of presumptive E coli O157 in meat and meat products was 35.55 percent (64/180), and 25.55 percent (46/180) and 21.66 percent (39/180) for water and vegetables respectively. Prevalence of presumptive E. coli O157 in the stool samples of HIV/AIDS patients was 36.39 percent (131/360), of which 56.5 percent (74/131) and 43.5 percent (57/131) were from stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Molecular analysis of representative presumptive E. coli O157 indicated that 10.29 percent (4/39) of vegetables; 14.81 percent (4/27) of water and 38.46 percent (5/13) of meat and meat products carried E. coli O157:H7. Also 36 percent (9/25) and 17.24 percent (5/29) of the stool samples were positive for E. coli O157:H7. Antimicrobial susceptibility profile revealed that all of the E. coli O157:H7 isolated from water, meat and meat products and vegetables as well as those isolated from stools of confirmed and non-confirmed HIV/AIDS patients were resistant (R) to gentamycin and erythromycin. However, 75 percent (20/27) of these isolates were resistant (R) to ampicillin and tetracycline whereas approximately 25 percent (6/27) were resistant (R) to nalidixic acid, ceftriaxone, and chloramphenicol. All the isolates (27/27) were susceptible (S) to amikacin. Probability of risk of E. coli O157:H7 infection was high for confirmed HIV/AIDS patients than for the non-confirmed HIV/AIDS patients. Estimated probability of risk of E. coli O157:H7 due to ingestion of water was 1.00 for 100 confirmed and non-confirmed HIV/AIDS patients. Risk due to meat and meat products was estimated at 0.27 and 0.20 and for vegetables at 0.21 and 0.15 per 100 confirmed and non-confirmed HIV/AIDS patients. The findings of this study predicted a possible link between E. coli O157:H7 isolated from drinking water, meat and meat products and vegetables and diarrhoeic conditions in both confirmed and non-confirmed HIV/AIDS patients, and concludes that confirmed HIV/AIDS patients can be at higher risk of contracting water and food borne E. coli O157:H7 than nonconfirmed HIV/AIDS patients. It is thus recommended that proper water treatment and food handling, maximum food and water safety and sanitation as well as personal body hygiene should be maintained, in order to prevent E. coli O157:H7 infections. Education initiatives and active surveillance of E. coli O157:H7 should be taken by all the stake-holders working directly or indirectly towards ensuring enduring sound public health.
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Date Issued: 2008

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Effect of processing on the efficacy and safety of Solanum Aculeastrum Dunal berries

- Aboyade, Oluwaseyi Mayode

Authors: Aboyade, Oluwaseyi Mayode
Date: 2009
Subjects: Medicinal plants -- South Africa -- Eastern Cape , Plant extracts , Drugs -- Effectiveness , Berries , Solanum , Solanum Aculeastrum -- Effectiveness
Language: English
Type: Thesis , Doctoral , PhD (Botany)
Identifier: vital:11302 , http://hdl.handle.net/10353/d1001051 , Medicinal plants -- South Africa -- Eastern Cape , Plant extracts , Drugs -- Effectiveness , Berries , Solanum , Solanum Aculeastrum -- Effectiveness
Description: General Abstract There has been a steady increase in the use of medicinal plants in the last two decades in both developed and developing countries for prevention, management and treatment of diseases. This increase has been due to reasons such as ease of access, better cultural acceptability and compatibility, cost effectiveness and also the bid to “go natural”. Unfortunately, the notion that herbal medicines are relatively safe because they are natural has led to serious and potentially fatal consequences in phytotherapy. The lack of rigorous research to prove the effectiveness and safety of many medicinal plants is of great concern to the health care system. This thesis therefore addresses not just the efficacy, but also the safety of the extracts of the berry of Solanum aculeastrum - a medicinal plant used, among other things, for the treatment of breast cancer in the Eastern Cape Province of South Africa. Particular attention was paid to the possible effect of different processing methods of the berry extracts on inflammation, cytotoxicity, and toxicity. In studying the comparative effects of various processing methods, four different preparations of the extracts were investigated. These include fresh, dried, boiled fresh, and boiled dried berries. While the effect of processing on the anti-inflammatory properties of the extracts was not dose dependent, the percentage reduction in inflammation was highly significant and more prominent in both concentrations of the boiled fresh berries than the reference drug, indomethacin. Furthermore, the four extracts varied in their ability to act either centrally or peripherally in their effect on pain. Assessment of the analgesic response using the formalin test showed that, at both concentrations tested, none of the extracts inhibited the first phase of the formalin test. Furthermore, it was observed that boiling had differing results on the activity of the iii fresh and dried extracts. While boiling of the dried berries reduced pain in the rats, the opposite trend was observed with the boiled fresh berries. Results of the influence of processing of the berries on cytotoxicity indicated that the extracts are potent inhibitors of human breast, cervical and colonic carcinoma cells and the non-cancerous cells (both the actively dividing and confluent Chang liver cells). Although, in terms of relative potency, the fresh berries appeared to be the most active of the extracts, processing of the berries caused an increase in apoptotic cells and a subsequent decrease in the necrotic cells. The effect of processing on the safety of the berry of S. aculeastrum on the rats fed for 28 days was also investigated. The various doses (1, 10 and 25 mg/kg body weight) of all the four extracts did not alter the activity and the weight of the animals throughout the period of treatment. A reduction in organ to body weight ratio of the heart, kidney, liver and spleen was observed in all the extracts. Regarding the haematological parameters, different patterns of effect were observed between the extracts and within the treated doses. The observed alterations in the biochemical parameters by the various extract of Solanum aculeastrum berries at all the doses may have consequential effects on the normal functioning of these organs. In conclusion, this study has shown that there is some justification for the traditional use of the berries of Solanum aculeastrum in the treatment of inflammation related ailments and cancer. However, the medicinal use of the plant also poses considerable health risks. Investigation conducted into the plant’s safety revealed that the berry extracts were nephrotoxic, hepatotoxic, haematotoxic and at higher doses, fatal. Another concern with regard to the plant’s safety is the non-selectivity of its extracts in the inhibition of carcinoma, actively dividing and un-dividing cells. Assessment of the effect of the processing on the berry’s efficacy and safety as herbal iv remedy produced mixed results. On the one hand, processing seemed to improve the extract’s anti-inflammatory and analgesic activity, while reducing its cytotoxic potential. On the other hand, a reduction in the toxicity was observed on the processed extracts compared to the fresh ones. This may be an indication that processing has an overall beneficial effect on the medicinal properties of the plant and should thus be considered as a method of making the berries of Solanum aculeastrum safer for use as a herbal remedy.
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Date Issued: 2009

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Isolation, purification and kinetic characterization of prolyl endopeptidase from Titicum aestivum

- Abrahams, Adriam Mark

Authors: Abrahams, Adriam Mark
Date: 2013
Subjects: Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
Language: English
Type: Thesis , Masters , MSc (Biochemistry)
Identifier: vital:11269 , http://hdl.handle.net/10353/d1004356 , Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
Description: PEP activity has been described in several locations and has mostly been linked to a variety of neurological disorders such as schizophrenia, amnaesia, depression as well as other disease states such as anorexia nervosa, bulimia nervosa and blood pressure regulation. The enzyme has also been previously isolated from a variety of archae, microorganisms and several eukaryotic species but no prolyl endopeptidases have been isolated from plants. Plants have high levels of proline and glutamine rich peptides in seeds. We therefore hypothesize plants must express PEPs during germination. Bioinformatics tools were used to identify known PEPs and putative plant PEPs. A global sequence alignment of putative plant PEPs and other known PEPs indicated that the active site amino acids Ser, His and Asp are conserved in putative plant PEP sequences. Furthermore, putative plant PEPs showed similar secondary structures to known PEPs and when a rice PEP was modelled onto porcine brain PEP structure, a high degree of similarity was found. Germination studies of wheat seed showed an increase of PEP activity over time with maximum PEP activity reached after 4 days of germination, which remained at this level until 9 days of germination, implying a function for PEP in plant seed germination. Wheat PEP was purified using ion exchange and gel filtration chromatography with a final yield of less than 1 percent and a relative purity (only 2 bands detected by SDS-PAGE). The purified wheat PEP had a molecular weight of approximately 55kDa, substrate specificity for chymotrypsin-like substrates (N-Suc-Ala-Ala-Pro-Phe-pNa, Km value of 0.58 mM, Kcat of 29.37 s–1; Kcat /Km 50813.14s–1 M–1); a pH optimum of 7.9; temperature optima of 37oC and a high sensitivity to temperature as indicated by loss of activity at temperatures above 40oC. Inhibition studies using E64, Leupeptin and PMSF confirmed that the wheat PEP is from the Serine protease family and is most likely a trypsin-like protease.
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Date Issued: 2013

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An investigation into factors that influence parental involvement in the development of their children's literacy in the secondary school level: a case study of a combined school in the Eastern Cape

- Adam, Ndileka Primrose

Authors: Adam, Ndileka Primrose
Date: 2010
Subjects: Education -- Parent participation -- South Africa -- Eastern Cape Province , Literacy -- South Africa -- Eastern Cape Province , Education, Secondary -- Parent participation -- South Africa -- Eastern Cape Province , Reading (Secondary) -- South Africa -- Eastern Cape Province , Reading -- Parent participation -- South Africa -- Eastern Cape Province , Reading -- Ability testing -- South Africa -- Eastern Cape Province
Language: English
Type: Thesis , Masters , M Ed
Identifier: vital:16158 , http://hdl.handle.net/10353/229 , Education -- Parent participation -- South Africa -- Eastern Cape Province , Literacy -- South Africa -- Eastern Cape Province , Education, Secondary -- Parent participation -- South Africa -- Eastern Cape Province , Reading (Secondary) -- South Africa -- Eastern Cape Province , Reading -- Parent participation -- South Africa -- Eastern Cape Province , Reading -- Ability testing -- South Africa -- Eastern Cape Province
Description: The main purpose of this research was to investigate the factors that influence parental involvement in the development of their children's literacy in the senior phase. The concept of parental involvement has been perceived as one of the corneThe main purpose of this research was to investigate the factors that influence parental involvement in the development of their children's literacy in the senior phase. The concept of parental involvement has been perceived as one of the cornerstones that contribute positively to the effectiveness of the education that children receive. In the South African case, it is embodied in the legislation and is expected to permeate the education process at all levels. Many scholars perceive parental involvement as enhancing the development of children's literacy. As an interpretive orientated study, this research had an interest in understanding the subjective experiences and general factors that influence parental involvement in the development of their children's literacy in the secondary school level. In line with the protocols of the case study, this study used a combination of methods namely structured interviews, semi-structured interviews, focus groups and document analysis in collecting data. The findings of the study revealed different opinions on parental involvement. While some parents appreciate the importance of their involvement, there were, however, some parents who perceive it as a responsibility of educators. Despite these views, there were positive contributions that have been brought about by parental involvement in the development of their children's literacy. The study has also revealed that there are factors that influence parental involvement in the development of their children's literacy in the senior phase such as parents' lack of understanding of their roles, absence of guiding documents, lack of unity among stakeholders, age and qualifications. The intent of this research was to investigate factors that influence parental involvement in the development of their children's literacy in the secondary school level and to give some recommendations on how these can best be addressed.rstones that contribute positively to the effectiveness of the education that children receive. In the South African case, it is embodied in the legislation and is expected to permeate the education process at all levels. Many scholars perceive parental involvement as enhancing the development of children's literacy. As an interpretive orientated study, this research had an interest in understanding the subjective experiences and general factors that influence parental involvement in the development of their children's literacy in the secondary school level. In line with the protocols of the case study, this study used a combination of methods namely structured interviews, semi-structured interviews, focus groups and document analysis in collecting data. The findings of the study revealed different opinions on parental involvement. While some parents appreciate the importance of their involvement, there were, however, some parents who perceive it as a responsibility of educators. Despite these views, there were positive contributions that have been brought about by parental involvement in the development of their children's literacy. The study has also revealed that there are factors that influence parental involvement in the development of their children's literacy in the senior phase such as parents' lack of understanding of their roles, absence of guiding documents, lack of unity among stakeholders, age and qualifications. The intent of this research was to investigate factors that influence parental involvement in the development of their children's literacy in the secondary school level and to give some recommendations on how these can best be addressed.
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Date Issued: 2010

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Fathers' involvement in the social development of children under six years: a social capital perspective and implications for early childhood development in the East London Education District

- Adams, Clyde Glenith Graham

Authors: Adams, Clyde Glenith Graham
Date: 2016
Subjects: Child development-- South Africa -- Eastern Cape Father and child -- South Africa -- Eastern Cape Fatherhood -- South Africa -- Eastern Cape
Language: English
Type: Thesis , Doctoral , Degree
Identifier: http://hdl.handle.net/10353/5116 , vital:29077
Description: The experiences of twenty fathers were explored to understand the involvement in the social development of children under six years. The main research question that guided the study was: what are fathers’ experiences with involvement in the social development of their children under the age of six years and what implications do these have for early childhood development? A social capital perspective was followed. The qualitative data was collected through the use of semi-structured interviews. The data was then analysed qualitatively. Results show that although most of the respondents could not give a definition of the concept of social development, they, however, understood the importance of parents playing a role in the development of a child. Fathers understood their role as supportive, nurturing and being there for their children. Fathers also understood their basic role as being the provider and protector. They also perceived their role as being authoritative and see themselves as the main disciplinarians. Results reveal that most schools do not offer programs specifically designed to encourage fathers to participate. Most importantly, results indicate that fathers’ interest in a variety of sporting activities, information sharing and talking about challenges both the schools as well as fathers face, are some of the ways fathers would like to be involved. Findings also indicate that although fathers acknowledged the role of the Department of Basic Education (DoBE) as mainly that of financial contributor in order to facilitate the skills development as well as that of information dissemination. However, fathers who took part in the study did not understand how the DoBE skills development agenda is envisaged for fathers. Notwithstanding, the respondents were quite clear that a father has an influential role to play in their children’s development. It also emerged that the greater the involvement, the more positive the influence on the child’s development. This study concludes that the presence of fathers in children’s lives is known to contribute significantly to their chances of experiencing positive developmental outcomes. Although the father-child relationship’s influences on children’s social competence have received increased attention in general, research on fathers’ understanding of their roles, involvement with their children as well as challenges confronting them with their involvement is scant. Based this the above, a number of recommendations have been made.
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Date Issued: 2016

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Case study of the educational experiences of four teenage mothers in two high schools in the Buffalo City Metropole

- Adams, Hermie E

Authors: Adams, Hermie E
Date: 2012
Subjects: Teenage girls -- Sexual behavior -- South Africa -- Eastern Cape , High schools -- South Africa -- Eastern Cape -- Social conditions
Language: English
Type: Thesis , Masters , M Ed
Identifier: vital:16168 , http://hdl.handle.net/10353/468 , Teenage girls -- Sexual behavior -- South Africa -- Eastern Cape , High schools -- South Africa -- Eastern Cape -- Social conditions
Description: Teenage pregnancy is a worldwide phenomenon. World Health Organisation (2009) reports that teenage mothers between the ages of 15-19 years account for 11% of births recorded worldwide. In South Africa it has been estimated that teenagers aged 17-19 account for 93% of all teenage pregnancies. A large proportion of these adolescents return to school after giving birth. Research has shown that there is a great deal of controversy about not only pre-marital sex, but also concerning whether teenage mothers should be allowed back in school at all. The aim of this study was to gain some insights into the lived experiences of teenage mothers in schools with particular reference to how they cope with school work and the responsibilities of motherhood. A case study of four teenage mothers who returned to school after giving birth was carried out. In-depth phenomenological interviewing designed to elicit the voices of the selected adolescents was done. There were five main findings. First, all four teenagers were minors, under the age of 18 when they gave birth. Two of them were even under the statutory age of consent. Second, upon return to school, teenage mothers experienced stigmatisation from peers and teachers and this forced them to continually negotiate their dual identities as mothers and learners. Third, teenage mothers experienced psychological emotions of stress; low self-esteem; shame and depression. Fourth, they also had sociological experiences in the form of material, financial and social support from family and friends. In some cases they experienced rejection from peers and abandonment by boyfriends who had made them pregnant. Some educators discriminated against and ridiculed teenage mothers. Fifth, teenage mothers reorganised their lives after childbirth and established routines that enabled them to cope with the demands of school work and the responsibilities of motherhood. iii The study concludes that, although viewed with scepticism by sections of the community and some educators, and given that some pregnancies are a result of abuse and unequal power relations between men and women in society, the policy of allowing teenage mothers back to school after giving birth gives them another chance to re-focus their lives. It is recommended that the voices of teenage mothers who return to school after childbirth should be taken into account to inform any planning for future policies on teenage pregnancy by schools and the state. It is further recommended that all educators should also be trained to be able to assist the teenage mothers instead of alienating them. There should also be counselling services available for the teenage mothers to enable them to deal with psychological and sociological problems they might encounter. For further research, students from different backgrounds should be the target of similar research. Another area of research should focus on academic performance of teenage mothers who return to school after giving birth. Lastly, there should be research that seeks to link what is taught in the Life Orientation curriculum and voices of teenage mothers.
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Date Issued: 2012

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A discourse analysis of the construction of gendered relationships in grade 10-12 Life Orientation textbooks in the Eastern Cape

- Adams, Luvo

Authors: Adams, Luvo
Date: 2017
Subjects: Sex instruction -- South Africa -- Eastern Cape Life skills -- Study and teaching (Secondary)
Language: English
Type: Thesis , Masters , MSoc
Identifier: http://hdl.handle.net/10353/5532 , vital:29315
Description: School-based sexuality education has been the subject of research in the social sciences and pedagogical spheres globally. In South Africa, growing interest among social scientists in the topic, were ignited by the introduction of sexuality education as a compulsory part of Life Orientation (LO) by the late 1990s. However, the implementation of LO has been problematic. Reviewed literature in the current study, reveals how the dominance of the heterosex discourse is foregrounded in LO content on gender and sexuality. The current study was aimed at examining the construction of gendered relationships in LO textbooks. The study sampled LO textbooks for Grades 10-12, learners in these grades are between the mean ages 16-18 years. This group is the target group, because they are legally afforded the right to consent to sexual activity with peers, within the same age bracket. Conducted from a social constructionist perspective, the current study employed qualitative methods of inquiry (textual analysis). Against the backdrop of heterosexuality as norm, it was the aim of the current study to understand the subject positions made available for female learners to construct themselves, within the discursive spaces in LO content. Findings suggest that two discourses namely: the heterosex discourse and the discourse of danger and disease, dominate in LO content on gender and sexuality. This leads to the construction of gendered relationships a s inherently heterosexual, leading to the marginalisation of relationships that fall outside of the norm. The female learner is positioned as a passive-victim, incapable of exercising sexual agency, while young men are positioned as inherently more powerful members of the intimate relationships or dangerous sexual predators. In the discourse of danger and disease, she is also positioned as a potential victim but the focus is on equipping her with skills, in a way which positioned her as an active-resistor in refusing sexual activity; and being in control of decision-making on issues of safety in relationships. The implications of these contradictions, is that they focus on the individual and disallow her taking up of sexual agency, and disregard the context in which she has to do so.
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Date Issued: 2017

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