Analytical Chemistry 1: PAC 222
- Authors: Katwire, D M , Ajibade, P A
- Date: 2011-01
- Language: English
- Type: Examination paper
- Identifier: vital:17814 , http://hdl.handle.net/10353/d1010366
- Description: Analytical Chemistry 1: PAC 222, supplementary examination January/February 2011.
- Full Text: false
- Date Issued: 2011-01
- Authors: Katwire, D M , Ajibade, P A
- Date: 2011-01
- Language: English
- Type: Examination paper
- Identifier: vital:17814 , http://hdl.handle.net/10353/d1010366
- Description: Analytical Chemistry 1: PAC 222, supplementary examination January/February 2011.
- Full Text: false
- Date Issued: 2011-01
Analytical Methods: MAP 221
- Authors: Makamba, B B , Mahlasela, Z
- Date: 2010-11
- Language: English
- Type: Examination paper
- Identifier: vital:17609 , http://hdl.handle.net/10353/d1009984
- Description: Analytical Methods: MAP 221, degree examination November 2010.
- Full Text: false
- Date Issued: 2010-11
- Authors: Makamba, B B , Mahlasela, Z
- Date: 2010-11
- Language: English
- Type: Examination paper
- Identifier: vital:17609 , http://hdl.handle.net/10353/d1009984
- Description: Analytical Methods: MAP 221, degree examination November 2010.
- Full Text: false
- Date Issued: 2010-11
Analytical Techniques in Agricultural Economics: AGE 411
- Authors: Mushunje, A , Kundhlane, G
- Date: 2011-08
- Subjects: Agriculture -- Economic aspects
- Language: English
- Type: Examination paper
- Identifier: vital:17650 , http://hdl.handle.net/10353/d1010029
- Description: Analytical Techniques in Agricultural Economics: AGE 411, degree examination August 2011.
- Full Text: false
- Date Issued: 2011-08
- Authors: Mushunje, A , Kundhlane, G
- Date: 2011-08
- Subjects: Agriculture -- Economic aspects
- Language: English
- Type: Examination paper
- Identifier: vital:17650 , http://hdl.handle.net/10353/d1010029
- Description: Analytical Techniques in Agricultural Economics: AGE 411, degree examination August 2011.
- Full Text: false
- Date Issued: 2011-08
Anatomy and Physiology: AGV 221
- Authors: Masika, P J , Marufu, M C
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17556 , http://hdl.handle.net/10353/d1009884
- Description: Anatomy and Physiology: AGV 221, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
- Authors: Masika, P J , Marufu, M C
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17556 , http://hdl.handle.net/10353/d1009884
- Description: Anatomy and Physiology: AGV 221, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
Anatomy and Physiology: AGV 221
- Authors: Masika, P J , Marufu, M C
- Date: 2010-02
- Subjects: Agriculture
- Language: English
- Type: Examination paper
- Identifier: vital:17523 , http://hdl.handle.net/10353/d1009805
- Description: Anatomy and Physiology: AGV 221, examination February 2010.
- Full Text: false
- Date Issued: 2010-02
- Authors: Masika, P J , Marufu, M C
- Date: 2010-02
- Subjects: Agriculture
- Language: English
- Type: Examination paper
- Identifier: vital:17523 , http://hdl.handle.net/10353/d1009805
- Description: Anatomy and Physiology: AGV 221, examination February 2010.
- Full Text: false
- Date Issued: 2010-02
Anatomy and Physiology: AGV 221
- Authors: Masika, P J , Marufu, M C
- Date: 2010-11
- Language: English
- Type: Examination paper
- Identifier: vital:17559 , http://hdl.handle.net/10353/d1009887
- Description: Anatomy and Physiology: AGV 221, degree examination November 2010.
- Full Text: false
- Date Issued: 2010-11
- Authors: Masika, P J , Marufu, M C
- Date: 2010-11
- Language: English
- Type: Examination paper
- Identifier: vital:17559 , http://hdl.handle.net/10353/d1009887
- Description: Anatomy and Physiology: AGV 221, degree examination November 2010.
- Full Text: false
- Date Issued: 2010-11
Animal Breeding: AGA 322
- Authors: Muchenje, V , Chimoyo, M
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17554 , http://hdl.handle.net/10353/d1009882
- Description: Animal Breeding: AGA 322, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
- Authors: Muchenje, V , Chimoyo, M
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17554 , http://hdl.handle.net/10353/d1009882
- Description: Animal Breeding: AGA 322, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
Animal Breeding: AGA 322
- Authors: Chimonyo, M , Muchenje, V
- Date: 2012-02
- Subjects: Animal Breeding
- Language: English
- Type: Examination paper
- Identifier: vital:17520 , http://hdl.handle.net/10353/d1009787
- Description: Animal Breeding: AGA 322, examination February 2010
- Full Text: false
- Date Issued: 2012-02
- Authors: Chimonyo, M , Muchenje, V
- Date: 2012-02
- Subjects: Animal Breeding
- Language: English
- Type: Examination paper
- Identifier: vital:17520 , http://hdl.handle.net/10353/d1009787
- Description: Animal Breeding: AGA 322, examination February 2010
- Full Text: false
- Date Issued: 2012-02
Animal Diversity and Conservation I: ZOO 314
- Masters, J, Genin, F, Parker, D
- Authors: Masters, J , Genin, F , Parker, D
- Date: 2011-06
- Language: English
- Type: Examination paper
- Identifier: vital:17787 , http://hdl.handle.net/10353/d1010327
- Description: Animal Diversity and Conservation I: ZOO 314, semester examination June 2011.
- Full Text: false
- Date Issued: 2011-06
- Authors: Masters, J , Genin, F , Parker, D
- Date: 2011-06
- Language: English
- Type: Examination paper
- Identifier: vital:17787 , http://hdl.handle.net/10353/d1010327
- Description: Animal Diversity and Conservation I: ZOO 314, semester examination June 2011.
- Full Text: false
- Date Issued: 2011-06
Animal Health, Infectious deseases: AGV 411
- Authors: Masika, P J , Zondi, M
- Date: 2011-06
- Language: English
- Type: Examination paper
- Identifier: vital:17549 , http://hdl.handle.net/10353/d1009877
- Description: Animal Health, Infectious deseases: AGV 411, degree examination June 2011.
- Full Text: false
- Date Issued: 2011-06
- Authors: Masika, P J , Zondi, M
- Date: 2011-06
- Language: English
- Type: Examination paper
- Identifier: vital:17549 , http://hdl.handle.net/10353/d1009877
- Description: Animal Health, Infectious deseases: AGV 411, degree examination June 2011.
- Full Text: false
- Date Issued: 2011-06
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Muchenje, V
- Date: 2011-01
- Language: English
- Type: Examination paper
- Identifier: vital:17532 , http://hdl.handle.net/10353/d1009828
- Description: Animal Nutrition: AGA 321, supplementary examination January 2011.
- Full Text: false
- Date Issued: 2011-01
- Authors: Nkukwana, T , Muchenje, V
- Date: 2011-01
- Language: English
- Type: Examination paper
- Identifier: vital:17532 , http://hdl.handle.net/10353/d1009828
- Description: Animal Nutrition: AGA 321, supplementary examination January 2011.
- Full Text: false
- Date Issued: 2011-01
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17527 , http://hdl.handle.net/10353/d1009812
- Description: Animal Nutrition: AGA 321, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17527 , http://hdl.handle.net/10353/d1009812
- Description: Animal Nutrition: AGA 321, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17552 , http://hdl.handle.net/10353/d1009880
- Description: Animal Nutrition: AGA 321, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Language: English
- Type: Examination paper
- Identifier: vital:17552 , http://hdl.handle.net/10353/d1009880
- Description: Animal Nutrition: AGA 321, degree examination November 2009.
- Full Text: false
- Date Issued: 2009-11
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17553 , http://hdl.handle.net/10353/d1009881
- Description: Animal Nutrition: AGA 321 Degree examination November 2009
- Full Text: false
- Date Issued: 2009-11
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17553 , http://hdl.handle.net/10353/d1009881
- Description: Animal Nutrition: AGA 321 Degree examination November 2009
- Full Text: false
- Date Issued: 2009-11
Antibacterial and phytochemical studies of selected South African honeys on clinical isolates of Helicobacter pylori
- Authors: Manyi-Loh, Christy E
- Date: 2012
- Subjects: Helicobacter pylori , Honey--South Africa , Drug resistance in microorganisms , Bacterial diseases , Honey -- Therapeutic use , Helicobacter pylori infections , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Microbiology)
- Identifier: vital:11240 , http://hdl.handle.net/10353/d1001056 , Helicobacter pylori , Honey--South Africa , Drug resistance in microorganisms , Bacterial diseases , Honey -- Therapeutic use , Helicobacter pylori infections , Traditional medicine -- South Africa -- Eastern Cape
- Description: Infection with Helicobacter pylori has been associated with the pathogenesis of numerous stomach and gastroduodenal diseases that pose threats to public health. Eradicaftion of this pathogen is a global challenge due to its alarming rate of multidrug resistance. Consequently, to find an alternative treatment, the search is increasingly focused on new antimicrobial product from natural sources including honey. Honey has been used as medicine in several cultures since ancient time due to its enormous biomedical activities. Its beneficial qualities have been endorsed to its antimicrobial, antioxidant, anti-inflammatory properties added to its phytocomponents. In this study, the anti-H. pylori activity of South African honeys and their solvent extracts as well as the phytochemicals present in the two most active honeys were evaluated. Agar well diffusion test was used to investigate the antimicrobial activity of six honey varieties obtained from different locations in the country. Subsequently, the honeys were extracted with four organic solvents viz n-hexane, diethyl ether, chloroform and ethyl acetate employed in order of increasing polarity. The antibacterial activity of the different solvent extracts of each honey was evaluated by agar well diffusion; broth micro dilution and time kill assays. Different chromatographic techniques (Thin layer & column chromatography) were employed to enumerate the phytochemical constituents in the most active solvent extracts of Pure Honey (PH) and Champagne Royal Train (CRT); and were identified by gas-chromatography linked mass-spectrometry. Linalool pure compound was equally evaluated for anti-H. pylori activity in a bid to trace the antibacterial agent among the variety of compounds identified. Data were analyzed by One-way ANOVA test at 95% confidence interval. Crude honeys and their solvent extracts demonstrated potent anti-H. pylori activity with zone diameter that ranged from [16.0mm (crude) to 22.2mm (extract)] and percentage susceptibilities of test isolates between 73.3% (crude) and 93.3% (extract). The chloroform extracts of PH and CRT were most active with MIC50 in the ranges 0.01- viii 10%v/v and 0.625-10%v/v respectively, not significantly different from amoxicillin (P> 0.05); and efficient bactericidal activity (100% bacterial cells killed) at 1/2MIC and 4xMIC over different time intervals, 36-72hrs and 18-72hrs respectively. The appearance of bands on the thin layer chromatography (TLC) chromatogram spotted with the chloroform extracts of PH and CRT; and developed with hexane: ethyl acetate: acetic acid (HEA) and methanol: acetic acid: water (MAAW) solvent systems indicated the presence of compounds. Purification of the compounds contained in these extracts over silica gel column yielded numerous fractions which were evaluated for antibacterial activity and purity. PHF5 was the most active fraction with a mean MIC50 value of 1.25mg/mL. Volatile compounds belonging to different known chemical families in honey were identified in all the active fractions obtained from PH. Conversely, only four compounds were identified in the active fractions obtained from CRT hence the non volatile constituents could be of prime relevance with respect to antibacterial activity of this honey. Of novelty was the presence of thiophene and N-methyl-D3-azirdine compounds, essential precursors used for the synthesis of natural products and pharmaceuticals with vital biomedical properties. Linalool demonstrated potent inhibitory (MIC95, 0.002- 0.0313mg/mL) and bactericidal activity (0.0039-0.313mg/mL) against the test isolates. On the other hand, a significant difference was recorded (P < 0.05) in comparing the activity of linalool compound to the fractions. PH could serve as a good economic source of bioactive compounds which could be employed as template for the synthesis of novel anti-H. pylori drugs. However, further studies are needed to determine the non volatile active ingredients in PH and CRT as well as toxicological testing
- Full Text:
- Date Issued: 2012
- Authors: Manyi-Loh, Christy E
- Date: 2012
- Subjects: Helicobacter pylori , Honey--South Africa , Drug resistance in microorganisms , Bacterial diseases , Honey -- Therapeutic use , Helicobacter pylori infections , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD (Microbiology)
- Identifier: vital:11240 , http://hdl.handle.net/10353/d1001056 , Helicobacter pylori , Honey--South Africa , Drug resistance in microorganisms , Bacterial diseases , Honey -- Therapeutic use , Helicobacter pylori infections , Traditional medicine -- South Africa -- Eastern Cape
- Description: Infection with Helicobacter pylori has been associated with the pathogenesis of numerous stomach and gastroduodenal diseases that pose threats to public health. Eradicaftion of this pathogen is a global challenge due to its alarming rate of multidrug resistance. Consequently, to find an alternative treatment, the search is increasingly focused on new antimicrobial product from natural sources including honey. Honey has been used as medicine in several cultures since ancient time due to its enormous biomedical activities. Its beneficial qualities have been endorsed to its antimicrobial, antioxidant, anti-inflammatory properties added to its phytocomponents. In this study, the anti-H. pylori activity of South African honeys and their solvent extracts as well as the phytochemicals present in the two most active honeys were evaluated. Agar well diffusion test was used to investigate the antimicrobial activity of six honey varieties obtained from different locations in the country. Subsequently, the honeys were extracted with four organic solvents viz n-hexane, diethyl ether, chloroform and ethyl acetate employed in order of increasing polarity. The antibacterial activity of the different solvent extracts of each honey was evaluated by agar well diffusion; broth micro dilution and time kill assays. Different chromatographic techniques (Thin layer & column chromatography) were employed to enumerate the phytochemical constituents in the most active solvent extracts of Pure Honey (PH) and Champagne Royal Train (CRT); and were identified by gas-chromatography linked mass-spectrometry. Linalool pure compound was equally evaluated for anti-H. pylori activity in a bid to trace the antibacterial agent among the variety of compounds identified. Data were analyzed by One-way ANOVA test at 95% confidence interval. Crude honeys and their solvent extracts demonstrated potent anti-H. pylori activity with zone diameter that ranged from [16.0mm (crude) to 22.2mm (extract)] and percentage susceptibilities of test isolates between 73.3% (crude) and 93.3% (extract). The chloroform extracts of PH and CRT were most active with MIC50 in the ranges 0.01- viii 10%v/v and 0.625-10%v/v respectively, not significantly different from amoxicillin (P> 0.05); and efficient bactericidal activity (100% bacterial cells killed) at 1/2MIC and 4xMIC over different time intervals, 36-72hrs and 18-72hrs respectively. The appearance of bands on the thin layer chromatography (TLC) chromatogram spotted with the chloroform extracts of PH and CRT; and developed with hexane: ethyl acetate: acetic acid (HEA) and methanol: acetic acid: water (MAAW) solvent systems indicated the presence of compounds. Purification of the compounds contained in these extracts over silica gel column yielded numerous fractions which were evaluated for antibacterial activity and purity. PHF5 was the most active fraction with a mean MIC50 value of 1.25mg/mL. Volatile compounds belonging to different known chemical families in honey were identified in all the active fractions obtained from PH. Conversely, only four compounds were identified in the active fractions obtained from CRT hence the non volatile constituents could be of prime relevance with respect to antibacterial activity of this honey. Of novelty was the presence of thiophene and N-methyl-D3-azirdine compounds, essential precursors used for the synthesis of natural products and pharmaceuticals with vital biomedical properties. Linalool demonstrated potent inhibitory (MIC95, 0.002- 0.0313mg/mL) and bactericidal activity (0.0039-0.313mg/mL) against the test isolates. On the other hand, a significant difference was recorded (P < 0.05) in comparing the activity of linalool compound to the fractions. PH could serve as a good economic source of bioactive compounds which could be employed as template for the synthesis of novel anti-H. pylori drugs. However, further studies are needed to determine the non volatile active ingredients in PH and CRT as well as toxicological testing
- Full Text:
- Date Issued: 2012
Antibacterial properties of the methanol extract of helichrysum pedunculatum
- Authors: Ncube, Nqobile S
- Date: 2008
- Subjects: Medicinal plants , Methanol , Helichrysum
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11241 , http://hdl.handle.net/10353/461 , Medicinal plants , Methanol , Helichrysum
- Description: The methanol extract of Helichrisum pedunculatum was screened for antimicrobial activity up to a concentration of 5 mg/ml using the agar dilution technique. A number of test bacterial isolates, comprising both Gram negative and Gram positive organisms were susceptible to the crude extract of the plant. The minimum inhibitory concentrations (MICs) of the extract ranged between 1 and 5 mg/ml for the susceptible organisms. The MICs of the selected antibiotics, chloramphenicol and penicillin, ranged between 2 and 4 mg/L, and 2 and 32 mg/L respectively against Bacillus cereus, Proteus vulgaris and Staphylococcus aureus OKOH1. Bactericidal activity was determined by the time kill assay. The methanol extract of the plant was not bactericidal at 1 × MIC for B. cereus, P. vulgaris and Staph. aureus OKOH1. At 2 × MIC the extract was bacteriostatic against B. cereus but bactericidal against P. vulgaris and Staph. aureus OKOH1. Combination studies were done at 1/2 × MIC, 1 × MIC and 2 × MIC of the plant extract with 1 × MIC of the antibiotics. Combinations of the plant extract and chloramphenicol resulted in mostly indifferent interactions against P. vulgaris and Staph. aureus OKOH1 but synergistic interactions at higher concentration of the plant extract for B. cereus. Penicillin combinations gave synergistic interactions at lower concentrations of the plant for P.vulgaris and Staph. aureus OKOH1 but was mostly indifferent for B. cereus.
- Full Text:
- Date Issued: 2008
- Authors: Ncube, Nqobile S
- Date: 2008
- Subjects: Medicinal plants , Methanol , Helichrysum
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11241 , http://hdl.handle.net/10353/461 , Medicinal plants , Methanol , Helichrysum
- Description: The methanol extract of Helichrisum pedunculatum was screened for antimicrobial activity up to a concentration of 5 mg/ml using the agar dilution technique. A number of test bacterial isolates, comprising both Gram negative and Gram positive organisms were susceptible to the crude extract of the plant. The minimum inhibitory concentrations (MICs) of the extract ranged between 1 and 5 mg/ml for the susceptible organisms. The MICs of the selected antibiotics, chloramphenicol and penicillin, ranged between 2 and 4 mg/L, and 2 and 32 mg/L respectively against Bacillus cereus, Proteus vulgaris and Staphylococcus aureus OKOH1. Bactericidal activity was determined by the time kill assay. The methanol extract of the plant was not bactericidal at 1 × MIC for B. cereus, P. vulgaris and Staph. aureus OKOH1. At 2 × MIC the extract was bacteriostatic against B. cereus but bactericidal against P. vulgaris and Staph. aureus OKOH1. Combination studies were done at 1/2 × MIC, 1 × MIC and 2 × MIC of the plant extract with 1 × MIC of the antibiotics. Combinations of the plant extract and chloramphenicol resulted in mostly indifferent interactions against P. vulgaris and Staph. aureus OKOH1 but synergistic interactions at higher concentration of the plant extract for B. cereus. Penicillin combinations gave synergistic interactions at lower concentrations of the plant for P.vulgaris and Staph. aureus OKOH1 but was mostly indifferent for B. cereus.
- Full Text:
- Date Issued: 2008
Antibiogram profiling of Escherichia coli pathotypes isolated from Kat River and Fort Beaufort abstraction water
- Authors: Nontongana, Nolonwabo
- Date: 2014
- Subjects: Escherichia coli infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11289 , http://hdl.handle.net/10353/d1019820 , Escherichia coli infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape
- Description: Escherichia coli (E. coli) is a widespread species that includes a broad variety of strains, ranging from highly pathogenic strains causing worldwide outbreaks of severe disease to virulent isolates which are part of the normal intestinal flora or which are well characterized and safe laboratory strains. The pathogenicity of a given E. coli strain is mainly determined by specific virulence factors which include adhesins, invasins, toxins and capsule. The aim of this study was to evaluate the prevalence and antibiogram profiles of E. coli pathotypes previously isolated from Kat River and Fort Beaufort abstraction water. A total of 171 E. coli isolates showed at least one pathogenic determinant among the isolated 278 E. coli. The other 107 isolates were negative for the tested virulence genes. All 278 presumptive isolates tested positive for the UidA gene, and were therefore classified as non-categorized pathogenic E. coli. The 171 pathogenic isolates had at least one characteristic gene of pathogenic E. coli and were identified and classified as enteropathogenic E. coli (6%), enterotoxigenicE. coli (131), uropathogenic E. coli (6), neonatal meningitis E. coli (14), diffusely adherent E. coli (1) and enterohaemrrhagic E. coli (1). Interestingly, no virulence genes were detected for the enteroinvasive E. coli and the enteroaggregative E. coli. The antibiotic resistance profiles for all isolates that were identified as E. coli showed 100% resistance to penicillin G, 98% resistance to ampicillin, 38% resistance to trimethoprim-sulphamethoxazole and 8% resistance to streptomycin. Multiple antibacterial resistance (MAR) was also observed, where 44% of the isolates were resistant to three antibiotics and 8% resistant to four antibiotics. The results of this study showed the Kat River and Fort Beaufort abstraction water are reservoirs of pathogenic strains of E. coli which harbour antibiotic resistance determinants that can cause serious health risks to the people in the surrounding communities.
- Full Text:
- Date Issued: 2014
- Authors: Nontongana, Nolonwabo
- Date: 2014
- Subjects: Escherichia coli infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11289 , http://hdl.handle.net/10353/d1019820 , Escherichia coli infections -- South Africa -- Eastern Cape , Water -- Pollution -- Environmental aspects -- South Africa -- Eastern Cape
- Description: Escherichia coli (E. coli) is a widespread species that includes a broad variety of strains, ranging from highly pathogenic strains causing worldwide outbreaks of severe disease to virulent isolates which are part of the normal intestinal flora or which are well characterized and safe laboratory strains. The pathogenicity of a given E. coli strain is mainly determined by specific virulence factors which include adhesins, invasins, toxins and capsule. The aim of this study was to evaluate the prevalence and antibiogram profiles of E. coli pathotypes previously isolated from Kat River and Fort Beaufort abstraction water. A total of 171 E. coli isolates showed at least one pathogenic determinant among the isolated 278 E. coli. The other 107 isolates were negative for the tested virulence genes. All 278 presumptive isolates tested positive for the UidA gene, and were therefore classified as non-categorized pathogenic E. coli. The 171 pathogenic isolates had at least one characteristic gene of pathogenic E. coli and were identified and classified as enteropathogenic E. coli (6%), enterotoxigenicE. coli (131), uropathogenic E. coli (6), neonatal meningitis E. coli (14), diffusely adherent E. coli (1) and enterohaemrrhagic E. coli (1). Interestingly, no virulence genes were detected for the enteroinvasive E. coli and the enteroaggregative E. coli. The antibiotic resistance profiles for all isolates that were identified as E. coli showed 100% resistance to penicillin G, 98% resistance to ampicillin, 38% resistance to trimethoprim-sulphamethoxazole and 8% resistance to streptomycin. Multiple antibacterial resistance (MAR) was also observed, where 44% of the isolates were resistant to three antibiotics and 8% resistant to four antibiotics. The results of this study showed the Kat River and Fort Beaufort abstraction water are reservoirs of pathogenic strains of E. coli which harbour antibiotic resistance determinants that can cause serious health risks to the people in the surrounding communities.
- Full Text:
- Date Issued: 2014
Antifugal evaluation and phytochemical analysis of selected medicinal plants used in the treatment of fungal diseases associated with HIV infection in the Eastern Cape Province, South Africa
- Authors: Mbeng, Wilfred Otang
- Date: 2013
- Subjects: Medicinal plants -- ethnobotanical survey , Opportunistic fungal infections , HIV/AIDS -- Cytotoxicity , Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Botany)
- Identifier: vital:11307 , http://hdl.handle.net/10353/d1006834 , Medicinal plants -- ethnobotanical survey , Opportunistic fungal infections , HIV/AIDS -- Cytotoxicity , Eastern Cape -- South Africa
- Description: Background. As a result of the AIDS pandemic, many people areimmuno compromised andopportunistic fungal infections (OFIs) such as candidiasis are common. Despite the widespread use of medicinal plants in South Africa, there is a dearth of knowledge regarding the use of such plants in the management of these infections. This study evaluates three South African medicinal plants (Arctotis arctotoides, Pittosporum viridiflorum, and Gasteria bicolor) traditionally used in the treatment of OFIs in HIV/AIDS patients, in the Eastern Cape Province, South Africa. Materials and methods. A six-stage process of documentation, evaluation and analysis of results was conducted: (1) Selection of medicinal plants most frequently used in the treatment of OFIs through ethnomedical studies and the survey of specialised literature; (2) Collection and preparation of the extract of each plant; (3) Antifungal evaluation of the crude plant extracts. (4) Phytochemical and antioxidant evaluation of the active crude plant extracts; (5) Cytotoxicity evaluation of the bioactive extracts using the Chang liver cell line, and (6) Statistical analysis of the results. Ethnobotanical information was obtained through interviews with traditional healers and AIDS patients with the aid of semi-structured questionnaires, direct observations and by reviewing studies reported in the literature. Following the approval from the University of Fort Hare‘s Ethics Committee, 101 HIV/AIDS patients were recruited through convenience sampling into an anonymous cross-sectional questionnaire study. The agar diffusion and micro-dilution methods were used to determine the antifungal activities of the hexane, acetone and aqueous extracts of A. arctotoides, G. bicolor and P. viridiflorum against 10 opportunistic fungi.
- Full Text:
- Date Issued: 2013
- Authors: Mbeng, Wilfred Otang
- Date: 2013
- Subjects: Medicinal plants -- ethnobotanical survey , Opportunistic fungal infections , HIV/AIDS -- Cytotoxicity , Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Botany)
- Identifier: vital:11307 , http://hdl.handle.net/10353/d1006834 , Medicinal plants -- ethnobotanical survey , Opportunistic fungal infections , HIV/AIDS -- Cytotoxicity , Eastern Cape -- South Africa
- Description: Background. As a result of the AIDS pandemic, many people areimmuno compromised andopportunistic fungal infections (OFIs) such as candidiasis are common. Despite the widespread use of medicinal plants in South Africa, there is a dearth of knowledge regarding the use of such plants in the management of these infections. This study evaluates three South African medicinal plants (Arctotis arctotoides, Pittosporum viridiflorum, and Gasteria bicolor) traditionally used in the treatment of OFIs in HIV/AIDS patients, in the Eastern Cape Province, South Africa. Materials and methods. A six-stage process of documentation, evaluation and analysis of results was conducted: (1) Selection of medicinal plants most frequently used in the treatment of OFIs through ethnomedical studies and the survey of specialised literature; (2) Collection and preparation of the extract of each plant; (3) Antifungal evaluation of the crude plant extracts. (4) Phytochemical and antioxidant evaluation of the active crude plant extracts; (5) Cytotoxicity evaluation of the bioactive extracts using the Chang liver cell line, and (6) Statistical analysis of the results. Ethnobotanical information was obtained through interviews with traditional healers and AIDS patients with the aid of semi-structured questionnaires, direct observations and by reviewing studies reported in the literature. Following the approval from the University of Fort Hare‘s Ethics Committee, 101 HIV/AIDS patients were recruited through convenience sampling into an anonymous cross-sectional questionnaire study. The agar diffusion and micro-dilution methods were used to determine the antifungal activities of the hexane, acetone and aqueous extracts of A. arctotoides, G. bicolor and P. viridiflorum against 10 opportunistic fungi.
- Full Text:
- Date Issued: 2013
Antioxidant activity and the quality of meat from goats and broilers supplemented with Moringa (Moringa Oleifera) leaves
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
- Authors: Qwele, Kumnandi
- Date: 2011
- Subjects: Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11803 , http://hdl.handle.net/10353/469 , Goats -- South Africa -- Eastern Cape , Chickens -- South Africa -- Eastern Cape , Meat -- Quality , Moringa
- Description: The objective of the study was to determine the antioxidant activity (AA) and the quality of meat from goats and chickens supplemented with Moringa (Moringa oleifera) leaves. For the first experimental chapter, eighteen 9 months old Xhosa lop-eared, castrated goats with an average body weight of 14 kg were used. There were three supplements namely, Moringa oleifera leaves, sunflower cake and grass hay. There were six goats per supplement. The goats were slaughtered after 60 days of supplementation and the Muscularis longissimus thoracis et lumborum (LTL) of the right side of each goat was used to determine fatty acid composition, total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) assay, 2, 2 azino-bis (3- ethylbenzothiazoline-6-sulphonic diammonium salt (ABTS) assay and reducing power assay of meat. Glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS) were taken as parameters to evaluate in vivo AA of the meat. For the second experiment, twelve male broilers were used in four groups of dietary supplementation. Three broilers were allocated per group. After slaughter, the breast muscle was sampled for total phenols, flavonoids, proanthocyanidins, TPC, ABTS, GSH, CAT, SOD, lipid peroxidation, ultimate pH (pH24), colour (L* (lightness), a* (redness), b* (yellowness)), WB-shear force and cooking loss. In both chapters Moringa leaves exhibited the highest AA. Meat from goats supplement with Moringa exhibited the highest AA. Meat from broilers supplemented with a mixture of Moringa leaves, broiler finisher and crushed maize, had the highest (P < 0.05) pH24 and L* values. Moringa can therefore be used to preserve meat without changing the quality of meat.
- Full Text:
- Date Issued: 2011
Application of assisted reproduction technologies on the indigenous Nguni cows and heifers
- Authors: Maqhashu, Ayanda
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11828 , http://hdl.handle.net/10353/d1016096
- Description: The aims of the study were to compare superovulatory (SO) response rate and embryo quality recovered; consequently, correlate sperm motility with fertilization rate on superovulated stud Nguni cows and heifers. Furthermore, compare oestrous synchronization response and pregnancy rate of three breed type cows (Brahman, Bonsmara and Nguni) of different body condition scores following timed artificial insemination in KwaZulu-Natal (KZN) and Limpopo provinces. Nguni stud cows (n= 15) and heifers (n= 10) aged 4-6 and 2-3 years were used as embryo donors. Superovulation of donors involved insertion of a controlled internal drug release device (CIDR) and two injections of FSH daily 12 hours apart for 4 days on a decreasing dosage. Fresh Nguni semen was collected from proven Nguni stud bulls and assessed by computer aided sperm analysis (CASA) before artificial insemination (AI). The doses of AI were prepared and conducted twice, 12 hours apart on synchronized and superovulated Nguni cows or heifers. Embryos were flushed 7 days after AI using a non-surgical technique. Embryos were immediately evaluated under stereo microscope and classified according IETS standard codes (C1, C1- and C2). All transferrable embryos were vitrified. Two pilot study sites were chosen in Eastern Cape – Great kei; (n= 5) and Limpopo - Vuvha; (n=5) provinces for Embryo transfer. Each recipient cow was implanted with one frozen-thawed embryo. For oestrous synchronization, cows (Braman, Bonsmara and Nguni type) were selected in different villages, regardless of parity, age, breed and body weight following pregnancy diagnosis. Cows were grouped according to breed type and body condition scores (BCS) on a scale of 1-5. Group 1 had BCS of ≤ 2.5 in KwaZulu-Natal (n=81) and Limpopo n=71), Group 2 had BCS of ≥ 3 in KwaZulu-Natal (n=79) and Limpopo (n=100) cows. Cows were synchronized by inserting the controlled intravaginal drug release (CIDR) and removed on Day 8, followed by administration of prostaglandin. The white heat mount detectors (HMD) were placed on the individual cow’s tail head as an indicator for oestrous response if colour changed to red and inseminated twice at 12 hours interval. Pregnancy diagnosis was performed by an ultra sound scanner and rectal palpation 90 days after TAI in embryos and semen recipient cows. There was no significant difference on the superovulatory response rate between Nguni cows (40%) and heifers (40%). There was a significant difference on the ovary reaction (number of corpus luteum) of cows (11.33±1.41) and heifers (4.00±0.57). There were no significant differences observed on the embryo quality between Nguni cows (2.5±1.00 and 1.25±0.59) and heifers (0.83±0.41 and 1.00±0.36) for excellent (C1) and good (C1-). However, cows had more numbers of unfertilized ova (5.5±1.05 and 1.75±0.47) and degenerate embryos (3.66±1.00 and 1.25±0.39) than heifers. Village cows responded to oestrous synchronization successfully in KZN (100%) and Limpopo (99%) regardless of body conditions and breed type. The lowest pregnancy rate was recorded in Brahman and Bonsmara type cows with BCS of ≤ 2.5 regardless of Province. Interestingly, Nguni type cows with same body condition of ≤ 2.5 had higher average pregnancy rate of 59.5% in Limpopo and 53.5% in KZN. However, cows with BCS of ≥3 had better pregnancy rate regardless of cow breed type, and province. In conclusion, only 40% of both Nguni cows and heifers responded to superovulation. However, Nguni cows had better ovaries reaction compared to heifers. The quality of embryos recovered was similar for both Nguni cows and heifers. Moreover, there was a positive correlation between total sperm motility and fertilization rate bull 1 (93.7%) inseminated the cows (67.5%) and bull 2 (83.5%) inseminated the heifers (53.5%). Higher pregnancy rate (60%) was recorded in Limpopo compared to Eastern Cape (0%). Interestingly, more than 99% of village cows responded to synchronization and inseminated with frozen-thawed semen successfully. Village Nguni type cows were not affected by body condition scoring as they had higher and similar pregnancy rate as those that had body condition of ≥ 3. It is suggested that it is not advisable to breed synchronized Brahman and Bonsmara type cows with the body condition of ≤ 2.5 except in Nguni cow type as more than 57% average pregnancy rate was achieved.
- Full Text:
- Date Issued: 2013
- Authors: Maqhashu, Ayanda
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11828 , http://hdl.handle.net/10353/d1016096
- Description: The aims of the study were to compare superovulatory (SO) response rate and embryo quality recovered; consequently, correlate sperm motility with fertilization rate on superovulated stud Nguni cows and heifers. Furthermore, compare oestrous synchronization response and pregnancy rate of three breed type cows (Brahman, Bonsmara and Nguni) of different body condition scores following timed artificial insemination in KwaZulu-Natal (KZN) and Limpopo provinces. Nguni stud cows (n= 15) and heifers (n= 10) aged 4-6 and 2-3 years were used as embryo donors. Superovulation of donors involved insertion of a controlled internal drug release device (CIDR) and two injections of FSH daily 12 hours apart for 4 days on a decreasing dosage. Fresh Nguni semen was collected from proven Nguni stud bulls and assessed by computer aided sperm analysis (CASA) before artificial insemination (AI). The doses of AI were prepared and conducted twice, 12 hours apart on synchronized and superovulated Nguni cows or heifers. Embryos were flushed 7 days after AI using a non-surgical technique. Embryos were immediately evaluated under stereo microscope and classified according IETS standard codes (C1, C1- and C2). All transferrable embryos were vitrified. Two pilot study sites were chosen in Eastern Cape – Great kei; (n= 5) and Limpopo - Vuvha; (n=5) provinces for Embryo transfer. Each recipient cow was implanted with one frozen-thawed embryo. For oestrous synchronization, cows (Braman, Bonsmara and Nguni type) were selected in different villages, regardless of parity, age, breed and body weight following pregnancy diagnosis. Cows were grouped according to breed type and body condition scores (BCS) on a scale of 1-5. Group 1 had BCS of ≤ 2.5 in KwaZulu-Natal (n=81) and Limpopo n=71), Group 2 had BCS of ≥ 3 in KwaZulu-Natal (n=79) and Limpopo (n=100) cows. Cows were synchronized by inserting the controlled intravaginal drug release (CIDR) and removed on Day 8, followed by administration of prostaglandin. The white heat mount detectors (HMD) were placed on the individual cow’s tail head as an indicator for oestrous response if colour changed to red and inseminated twice at 12 hours interval. Pregnancy diagnosis was performed by an ultra sound scanner and rectal palpation 90 days after TAI in embryos and semen recipient cows. There was no significant difference on the superovulatory response rate between Nguni cows (40%) and heifers (40%). There was a significant difference on the ovary reaction (number of corpus luteum) of cows (11.33±1.41) and heifers (4.00±0.57). There were no significant differences observed on the embryo quality between Nguni cows (2.5±1.00 and 1.25±0.59) and heifers (0.83±0.41 and 1.00±0.36) for excellent (C1) and good (C1-). However, cows had more numbers of unfertilized ova (5.5±1.05 and 1.75±0.47) and degenerate embryos (3.66±1.00 and 1.25±0.39) than heifers. Village cows responded to oestrous synchronization successfully in KZN (100%) and Limpopo (99%) regardless of body conditions and breed type. The lowest pregnancy rate was recorded in Brahman and Bonsmara type cows with BCS of ≤ 2.5 regardless of Province. Interestingly, Nguni type cows with same body condition of ≤ 2.5 had higher average pregnancy rate of 59.5% in Limpopo and 53.5% in KZN. However, cows with BCS of ≥3 had better pregnancy rate regardless of cow breed type, and province. In conclusion, only 40% of both Nguni cows and heifers responded to superovulation. However, Nguni cows had better ovaries reaction compared to heifers. The quality of embryos recovered was similar for both Nguni cows and heifers. Moreover, there was a positive correlation between total sperm motility and fertilization rate bull 1 (93.7%) inseminated the cows (67.5%) and bull 2 (83.5%) inseminated the heifers (53.5%). Higher pregnancy rate (60%) was recorded in Limpopo compared to Eastern Cape (0%). Interestingly, more than 99% of village cows responded to synchronization and inseminated with frozen-thawed semen successfully. Village Nguni type cows were not affected by body condition scoring as they had higher and similar pregnancy rate as those that had body condition of ≥ 3. It is suggested that it is not advisable to breed synchronized Brahman and Bonsmara type cows with the body condition of ≤ 2.5 except in Nguni cow type as more than 57% average pregnancy rate was achieved.
- Full Text:
- Date Issued: 2013