Photocatalytic activity and antibacterial properties of Ag/N-doped TiO2 nanoparticles on PVAE-CS nanofibre support
- Authors: Ocwelwang, Atsile Rosy
- Date: 2012
- Subjects: Nanocomposites (Materials) , Nanofibers , Electrospinning
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11337 , http://hdl.handle.net/10353/d1006794 , Nanocomposites (Materials) , Nanofibers , Electrospinning
- Description: Lack of potable water is one of the major challenges that the world faces currently and the effects of this are mainly experienced by people in developing countries. This has therefore propelled research in advanced oxidation technologies AOTs to improve the current water treatment methods using cost effective, non toxic and efficient treatment methods. Hence, in this study the sol-gel synthesis method was used to prepare TiO2 nanoparticles that were photocatalytically active under UV and visible solar light as well as possessing antibacterial properties. Silver and nitrogen doping was carried out to extend the optical absorption of TiO2. For easy removal and reuse of the photocatalyst the nanoparticles were immobilized on chitosan and poly (vinyl-alcohol-co-ethylene) using the electrospining technique. The synthesized nanomaterials were characterized by FTIR, XRD, SEM/EDS, TEM, DRS, and TGA. FTIR and EDS analysis confirmed the formation and composition of TiO2 nanopowders for the doped and undoped nanoparticles. XRD analysis showed that the anatase phase was the dominant crystalline phase of the synthesized nanopowders. SEM and TEM respectively illustrated the distribution and size of the electrospun nanofibers and the nanoparticles of TiO2. DRS results showed that there was a significant shift in the absorption band edge and wavelength of Ag-TiO2 to 397 nm, followed by N-TiO2 at 396 nm compared to the commercial titania which was at 359 nm. The photocatalytic activities and antibacterial properties of these materials were tested on methylene blue dye and E.coli microorganism respectively. Ag-TiO2 immobilized on nanofibers of chitosan and PVAE had the highest photocatalytic activity compared to N-TiO2. Similar results were observed when the biocide properties of these materials were tested on E. coli.
- Full Text:
- Authors: Ocwelwang, Atsile Rosy
- Date: 2012
- Subjects: Nanocomposites (Materials) , Nanofibers , Electrospinning
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11337 , http://hdl.handle.net/10353/d1006794 , Nanocomposites (Materials) , Nanofibers , Electrospinning
- Description: Lack of potable water is one of the major challenges that the world faces currently and the effects of this are mainly experienced by people in developing countries. This has therefore propelled research in advanced oxidation technologies AOTs to improve the current water treatment methods using cost effective, non toxic and efficient treatment methods. Hence, in this study the sol-gel synthesis method was used to prepare TiO2 nanoparticles that were photocatalytically active under UV and visible solar light as well as possessing antibacterial properties. Silver and nitrogen doping was carried out to extend the optical absorption of TiO2. For easy removal and reuse of the photocatalyst the nanoparticles were immobilized on chitosan and poly (vinyl-alcohol-co-ethylene) using the electrospining technique. The synthesized nanomaterials were characterized by FTIR, XRD, SEM/EDS, TEM, DRS, and TGA. FTIR and EDS analysis confirmed the formation and composition of TiO2 nanopowders for the doped and undoped nanoparticles. XRD analysis showed that the anatase phase was the dominant crystalline phase of the synthesized nanopowders. SEM and TEM respectively illustrated the distribution and size of the electrospun nanofibers and the nanoparticles of TiO2. DRS results showed that there was a significant shift in the absorption band edge and wavelength of Ag-TiO2 to 397 nm, followed by N-TiO2 at 396 nm compared to the commercial titania which was at 359 nm. The photocatalytic activities and antibacterial properties of these materials were tested on methylene blue dye and E.coli microorganism respectively. Ag-TiO2 immobilized on nanofibers of chitosan and PVAE had the highest photocatalytic activity compared to N-TiO2. Similar results were observed when the biocide properties of these materials were tested on E. coli.
- Full Text:
Perceived organisational support (POS), Job engagement (JE) and their effect on organisational citizenship behaviour (OCB) among nurses at the Victoria Hospital, Alice, Nkonkobe Municipality
- Authors: Dumisani, Mathumbu
- Date: 2012
- Subjects: Municipal government -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Organizational behavior -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nursing -- South Africa -- Eastern Cape , Job stress -- South Africa -- Eastern Cape , Employee retention -- South Africa -- Eastern Cape , Employees -- Training of -- South Africa -- Eastern Cape , Perceived Organization Support , Job Engagement , Organizational Citizenship Behavior
- Language: English
- Type: Thesis , Masters , MCom (Industrial Psychology)
- Identifier: vital:11552 , http://hdl.handle.net/10353/d1007032 , Municipal government -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Organizational behavior -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nursing -- South Africa -- Eastern Cape , Job stress -- South Africa -- Eastern Cape , Employee retention -- South Africa -- Eastern Cape , Employees -- Training of -- South Africa -- Eastern Cape , Perceived Organization Support , Job Engagement , Organizational Citizenship Behavior
- Description: Wendel (1994, p. 91) defined perceived organizational support (POS) as “organization support in terms of all things that relate to assistance and relationships amongst working peers and colleagues, which involve the feeling of need between superiors and subordinates . Rothbard (2001, p. 656) in defining job engagement (JE) listed two components which he thought were critical for its effectiveness on organizational functioning: (i) attention and (ii) absorption, with the former referring to “cognitive ability and the amount of time one spends thinking about the role”, while the latter “means being engrossed in a role and means the intensity of one’s focus on a role”. Organizational citizenship behavior (OCB) was first introduced by Organ in the 1980s and he defined the concept of organizational citizenship behavior “as discretionary behaviors by individuals (employees) that do not form part of formal requirements of a job, but are necessary and promote effective functioning of the organization (Organ, 1988)”. The objective of study was to explore the relationship between perceived organizational support and job engagement and their effect organizational citizenship behavior. Other relationships that were tested were first, the direct relationship between POS JE. Secondly, the combined effect of POS and JE on OCB. The study was conducted amongst nurses at Victoria hospital, in Alice within the Nkonkobe district municipality. The results showed a significant relationship between JE and OCB, whilst the relationship between POS and OCB was not accepted. The results for the other two hypotheses that were tested; (i) relationship between POS and JE, (ii) combined effect of POS and JE on OCB also showed that they were not accepted. The consistency scores for these variables were of international level (n=106). The Pearson correlation coefficients were used for hypothesis testing.
- Full Text:
- Authors: Dumisani, Mathumbu
- Date: 2012
- Subjects: Municipal government -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Organizational behavior -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nursing -- South Africa -- Eastern Cape , Job stress -- South Africa -- Eastern Cape , Employee retention -- South Africa -- Eastern Cape , Employees -- Training of -- South Africa -- Eastern Cape , Perceived Organization Support , Job Engagement , Organizational Citizenship Behavior
- Language: English
- Type: Thesis , Masters , MCom (Industrial Psychology)
- Identifier: vital:11552 , http://hdl.handle.net/10353/d1007032 , Municipal government -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Organizational behavior -- South Africa -- Eastern Cape , Nurses -- South Africa -- Eastern Cape , Nursing -- South Africa -- Eastern Cape , Job stress -- South Africa -- Eastern Cape , Employee retention -- South Africa -- Eastern Cape , Employees -- Training of -- South Africa -- Eastern Cape , Perceived Organization Support , Job Engagement , Organizational Citizenship Behavior
- Description: Wendel (1994, p. 91) defined perceived organizational support (POS) as “organization support in terms of all things that relate to assistance and relationships amongst working peers and colleagues, which involve the feeling of need between superiors and subordinates . Rothbard (2001, p. 656) in defining job engagement (JE) listed two components which he thought were critical for its effectiveness on organizational functioning: (i) attention and (ii) absorption, with the former referring to “cognitive ability and the amount of time one spends thinking about the role”, while the latter “means being engrossed in a role and means the intensity of one’s focus on a role”. Organizational citizenship behavior (OCB) was first introduced by Organ in the 1980s and he defined the concept of organizational citizenship behavior “as discretionary behaviors by individuals (employees) that do not form part of formal requirements of a job, but are necessary and promote effective functioning of the organization (Organ, 1988)”. The objective of study was to explore the relationship between perceived organizational support and job engagement and their effect organizational citizenship behavior. Other relationships that were tested were first, the direct relationship between POS JE. Secondly, the combined effect of POS and JE on OCB. The study was conducted amongst nurses at Victoria hospital, in Alice within the Nkonkobe district municipality. The results showed a significant relationship between JE and OCB, whilst the relationship between POS and OCB was not accepted. The results for the other two hypotheses that were tested; (i) relationship between POS and JE, (ii) combined effect of POS and JE on OCB also showed that they were not accepted. The consistency scores for these variables were of international level (n=106). The Pearson correlation coefficients were used for hypothesis testing.
- Full Text:
An investigation into the implementation of the life orientation curriculum in selected Fort Beaufort district high schools
- Authors: Adewumi, Toyin Mary
- Date: 2012
- Subjects: Life skills -- South Africa -- Eastern Cape , Curriculum-based assessment -- South Africa -- Eastern Cape , Curriculum planning -- South Africa -- Eastern Cape , High school teaching -- South Africa -- Eastern Cape , Teaching -- South Africa -- Eastern Cape -- Aids and devices , Teachers -- Job stress -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Ed
- Identifier: vital:16174 , http://hdl.handle.net/10353/541 , Life skills -- South Africa -- Eastern Cape , Curriculum-based assessment -- South Africa -- Eastern Cape , Curriculum planning -- South Africa -- Eastern Cape , High school teaching -- South Africa -- Eastern Cape , Teaching -- South Africa -- Eastern Cape -- Aids and devices , Teachers -- Job stress -- South Africa -- Eastern Cape
- Description: The challenges of implementing the curriculum for the Life Orientation learning areas in the National Curriculum Statement have been a matter of concern for South African schools. This study sheds light on the implementation of the LO curriculum in selected high schools in the Fort Beaufort District, in the Eastern Cape Province in South Africa. The study adopted a qualitative approach and used face-to-face interviews, focus group interviews, classroom observation and document analysis to collect the data. The purpose of the study was to investigate the implementation of the Life Orientation curriculum in high schools in the Fort Beaufort District. Six high schools from the six clusters in the Fort Beaufort District were purposively selected to form the focus of the study. The participants were six principals, six LO teachers and thirty-six learners. The study revealed that lack of adequate teaching and learning materials in schools affected the implementation of the curriculum. Inadequacy of teaching and learning materials affected both the teachers and learners as they sought to make up for the shortages of teaching and learning materials by sourcing for local materials like news paper and magazine. In some of the schools, teachers made photocopies of textbooks for learners which could be time consuming and added to the teachers‘ workload. The study found that some of the teachers were frustrated because of the lack of adequate teaching and learning materials in LO. The results also revealed that the majority of the teachers had the Advanced Certificate in Education (ACE LO) qualification and/or an undergraduate qualification in Psychology, which are basic requirement for teaching LO, while the others had general teaching qualifications in other subjects. It was found that many learners were positive about LO, but it could not be proven that learners meant all their positive responses as it seemed that learners‘ responses were too good to be true. However, the results showed that learners had at least been taught and were aware of the consequences of the social problems like teenage pregnancy, drug abuse and HIV/AIDS that affect them. Some of the challenges that teachers encountered in the implementation of the Life Orientation curriculum were also highlighted in this study. These include; lack of adequate training which some of the teachers complained did not allow them to handle some topics like career choice and religious education as they should. Some of the teachers also stated that their culture and belief did not permit them to share some LO topics with their learners. There was also the challenge of learners turning against things taught in LO class. The study found that the support and monitoring put in place for LO teachers are in the form of documents such as subject guides, textbooks and sometimes funds to take learners out on outdoor activities, extra teachers to lessen the teachers‘ burdens, advice, workshops and training. There is course moderation where teachers‘ and learners‘ files are marked. In some of the schools where monitoring is carried out, it starts with the Heads of Departments (HODs) and ends with the principal. Many of the schools do not monitor the teaching of LO because of the trust the principals have in the teachers and challenge of school size. Class visits is prohibited by South African Democratic Teachers‘ Union. The subject is not being handled properly despite its importance in helping learners to be adequately guided towards positive self-concept formation, the realisation of their potential, and enabling them to protect themselves from various forms of social violence and abuse, and this in turn would make the society safer.
- Full Text:
- Authors: Adewumi, Toyin Mary
- Date: 2012
- Subjects: Life skills -- South Africa -- Eastern Cape , Curriculum-based assessment -- South Africa -- Eastern Cape , Curriculum planning -- South Africa -- Eastern Cape , High school teaching -- South Africa -- Eastern Cape , Teaching -- South Africa -- Eastern Cape -- Aids and devices , Teachers -- Job stress -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Ed
- Identifier: vital:16174 , http://hdl.handle.net/10353/541 , Life skills -- South Africa -- Eastern Cape , Curriculum-based assessment -- South Africa -- Eastern Cape , Curriculum planning -- South Africa -- Eastern Cape , High school teaching -- South Africa -- Eastern Cape , Teaching -- South Africa -- Eastern Cape -- Aids and devices , Teachers -- Job stress -- South Africa -- Eastern Cape
- Description: The challenges of implementing the curriculum for the Life Orientation learning areas in the National Curriculum Statement have been a matter of concern for South African schools. This study sheds light on the implementation of the LO curriculum in selected high schools in the Fort Beaufort District, in the Eastern Cape Province in South Africa. The study adopted a qualitative approach and used face-to-face interviews, focus group interviews, classroom observation and document analysis to collect the data. The purpose of the study was to investigate the implementation of the Life Orientation curriculum in high schools in the Fort Beaufort District. Six high schools from the six clusters in the Fort Beaufort District were purposively selected to form the focus of the study. The participants were six principals, six LO teachers and thirty-six learners. The study revealed that lack of adequate teaching and learning materials in schools affected the implementation of the curriculum. Inadequacy of teaching and learning materials affected both the teachers and learners as they sought to make up for the shortages of teaching and learning materials by sourcing for local materials like news paper and magazine. In some of the schools, teachers made photocopies of textbooks for learners which could be time consuming and added to the teachers‘ workload. The study found that some of the teachers were frustrated because of the lack of adequate teaching and learning materials in LO. The results also revealed that the majority of the teachers had the Advanced Certificate in Education (ACE LO) qualification and/or an undergraduate qualification in Psychology, which are basic requirement for teaching LO, while the others had general teaching qualifications in other subjects. It was found that many learners were positive about LO, but it could not be proven that learners meant all their positive responses as it seemed that learners‘ responses were too good to be true. However, the results showed that learners had at least been taught and were aware of the consequences of the social problems like teenage pregnancy, drug abuse and HIV/AIDS that affect them. Some of the challenges that teachers encountered in the implementation of the Life Orientation curriculum were also highlighted in this study. These include; lack of adequate training which some of the teachers complained did not allow them to handle some topics like career choice and religious education as they should. Some of the teachers also stated that their culture and belief did not permit them to share some LO topics with their learners. There was also the challenge of learners turning against things taught in LO class. The study found that the support and monitoring put in place for LO teachers are in the form of documents such as subject guides, textbooks and sometimes funds to take learners out on outdoor activities, extra teachers to lessen the teachers‘ burdens, advice, workshops and training. There is course moderation where teachers‘ and learners‘ files are marked. In some of the schools where monitoring is carried out, it starts with the Heads of Departments (HODs) and ends with the principal. Many of the schools do not monitor the teaching of LO because of the trust the principals have in the teachers and challenge of school size. Class visits is prohibited by South African Democratic Teachers‘ Union. The subject is not being handled properly despite its importance in helping learners to be adequately guided towards positive self-concept formation, the realisation of their potential, and enabling them to protect themselves from various forms of social violence and abuse, and this in turn would make the society safer.
- Full Text:
Sedimentary environments and provenance of the Balfour Formation (Beaufort Group) in the area between Bedford and Adelaide, Eastern Cape Province, South Africa
- Authors: Oghenekome, Monica Enifome
- Date: 2012
- Subjects: Geology -- South Africa -- Balfour , Sedimentary basins -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape , Sedimentation analysis , Beaufort Group (South Africa)
- Language: English
- Type: Thesis , Masters , MSc (Geology)
- Identifier: vital:11524 , http://hdl.handle.net/10353/d1004354 , Geology -- South Africa -- Balfour , Sedimentary basins -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape , Sedimentation analysis , Beaufort Group (South Africa)
- Description: The research examines the sedimentary environments and provenance of the Balfour Formation of the Beaufort Group (Karoo Supergroup) in the Eastern Cape Province, South Africa. This Formation occurs in the southeastern part of the Karoo Basin. It consists of sedimentary rocks, which are an alternating siltstone, shale and mudstone succession with subordinate interbedded sandstone and subsequently intruded by Karoo dolerite in the form of sills and dykes. ithostratigraphically, the Balfour Formation is subdivided into five units namely, from the base to the top, the Oudeberg, Daggaboersnek, Barberskrans, Elandsberg and Palingkloof Members. The Balfour Formation is overlain by the Katberg Formation. This study involved field investigations in the vicinity of the towns of Bedford and Adelaide with integrated stratigraphical, sedimentological and petrological studies. A geological map was constructed after field investigations. Lithofacies of the Balfour Formation that were studied are characterised by sandstone facies (Sh, Sm, St, Sr, Sp) and fine-grained sediments (Fl or Fsm) which reflect point-bar, cut-bank, channel and floodplain deposits. Lithologically, the Oudeberg Member consists of sandstone of which some units are internally massive alternating with thin laminated siltstone and mudstone. The Daggaboersnek Member is characterised by regular, generally non-lenticular, overall stratification, in the Barberkrans Member consists of sandstone lithosomes, while the Elandsberg Member is an argillaceous unit, similar to the Daggaboersnek Member. The Palingkloof Member is composed predominantly of red mudstone that can be used to distinguish the Balfour Formation from the overlying Katberg Formation, which consists predominantly of sandstone. The stratigraphic sequence displays two fining upward megacycles of sedimentary deposits with change in the sediment supply pattern from low-sinuosity to high-sinuosity river systems which reflect both braid and meandering deposits, respectively. Sedimentary structures in the sandstone units and the provenance of the Balfour Formation indicate that these deposits were produced by rivers flowing from the southeast with minor drift towards the northwest. According to the composition of the sediments and their sequence of deposition the Formation represents a fluvial environment. Mineralogical and grain size data from the sandstones of the various members of the Balfour Formation indicate the same source area of granitic, metamorphic and older sedimentary rocks and show no significant petrographic differences. The petrographic and geochemical investigations confirmed the sandstone to be feldspathic litharenite and ultralithofeldspathic sandstone. The palaeocurrent investigation indicates the main provenance to have been situated to the southeast of the Karoo basin. Heavy-mineral concentrations within the sandstones also give an indication that the source had a transitional arc plate tectonic setting.
- Full Text:
- Authors: Oghenekome, Monica Enifome
- Date: 2012
- Subjects: Geology -- South Africa -- Balfour , Sedimentary basins -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape , Sedimentation analysis , Beaufort Group (South Africa)
- Language: English
- Type: Thesis , Masters , MSc (Geology)
- Identifier: vital:11524 , http://hdl.handle.net/10353/d1004354 , Geology -- South Africa -- Balfour , Sedimentary basins -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape , Sedimentation analysis , Beaufort Group (South Africa)
- Description: The research examines the sedimentary environments and provenance of the Balfour Formation of the Beaufort Group (Karoo Supergroup) in the Eastern Cape Province, South Africa. This Formation occurs in the southeastern part of the Karoo Basin. It consists of sedimentary rocks, which are an alternating siltstone, shale and mudstone succession with subordinate interbedded sandstone and subsequently intruded by Karoo dolerite in the form of sills and dykes. ithostratigraphically, the Balfour Formation is subdivided into five units namely, from the base to the top, the Oudeberg, Daggaboersnek, Barberskrans, Elandsberg and Palingkloof Members. The Balfour Formation is overlain by the Katberg Formation. This study involved field investigations in the vicinity of the towns of Bedford and Adelaide with integrated stratigraphical, sedimentological and petrological studies. A geological map was constructed after field investigations. Lithofacies of the Balfour Formation that were studied are characterised by sandstone facies (Sh, Sm, St, Sr, Sp) and fine-grained sediments (Fl or Fsm) which reflect point-bar, cut-bank, channel and floodplain deposits. Lithologically, the Oudeberg Member consists of sandstone of which some units are internally massive alternating with thin laminated siltstone and mudstone. The Daggaboersnek Member is characterised by regular, generally non-lenticular, overall stratification, in the Barberkrans Member consists of sandstone lithosomes, while the Elandsberg Member is an argillaceous unit, similar to the Daggaboersnek Member. The Palingkloof Member is composed predominantly of red mudstone that can be used to distinguish the Balfour Formation from the overlying Katberg Formation, which consists predominantly of sandstone. The stratigraphic sequence displays two fining upward megacycles of sedimentary deposits with change in the sediment supply pattern from low-sinuosity to high-sinuosity river systems which reflect both braid and meandering deposits, respectively. Sedimentary structures in the sandstone units and the provenance of the Balfour Formation indicate that these deposits were produced by rivers flowing from the southeast with minor drift towards the northwest. According to the composition of the sediments and their sequence of deposition the Formation represents a fluvial environment. Mineralogical and grain size data from the sandstones of the various members of the Balfour Formation indicate the same source area of granitic, metamorphic and older sedimentary rocks and show no significant petrographic differences. The petrographic and geochemical investigations confirmed the sandstone to be feldspathic litharenite and ultralithofeldspathic sandstone. The palaeocurrent investigation indicates the main provenance to have been situated to the southeast of the Karoo basin. Heavy-mineral concentrations within the sandstones also give an indication that the source had a transitional arc plate tectonic setting.
- Full Text:
Evaluation of rural sanitation in Buffalo City Metropolitan Municipality
- Authors: Marata, Mawethu Nicolas
- Date: 2012
- Subjects: Sanitation, Rural -- South Africa -- Eastern Cape , Sanitation -- South Africa -- Eastern Cape , Public utilities -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Buffalo City (South Africa)
- Language: English
- Type: Thesis , Masters , M Soc Sc (Rural Development)
- Identifier: vital:11944 , http://hdl.handle.net/10353/d1005966 , Sanitation, Rural -- South Africa -- Eastern Cape , Sanitation -- South Africa -- Eastern Cape , Public utilities -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Buffalo City (South Africa)
- Description: This research paper is a qualitative evaluation of rural resident’s views in Buffalo City Metropolitan Municipality regarding the municipalities (BCM) performance in sanitation in rural areas. The study was conducted using interviews and some participant observation as a strategy for data collection. The data collected was analyzed using the basic needs theoretical framework. The study found that sanitation is the least talked about subject because of the stigma attached to it. The study found that Buffalo City Municipality initiatives in sanitation have little impact or has not yet transformed the lives of rural people. It has been found that the municipality is responding slowly in sanitation, and this situation impacts negatively on the lives of rural people as they are exposed to health hazards and diseases. The study questions the sustainability of toilets built in rural areas as many toilets have no roofs and door. It has been found that sanitation is still a challenge in the Buffalo City Municipality as some people still relieve themselves in the bushes. Some people even defecate into plastic bags and then throw the bags as far as they can. The study found that the environment is deeply affected by the lack of sanitation as both air and water are contaminated by waste disposal caused by poor sanitation. The general feeling of the residents of Buffalo City Municipality with regard to sanitation service delivery is that they were not happy at all. The residents are not happy with the state of sanitation services rendered by the municipality. The study calls upon the municipality to speedily address rural sanitation to avoid service delivery protest. The research calls upon organizations and companies to get sanitation issues as part of their function. The research made a number of recommendations in an effort to help municipalities accelerate the delivery of sanitation in rural areas. It is hoped that the recommendations would help the Buffalo City Municipality in ensuring that communities are equipped and empowered to meaningfully participate in making policies, regarding their development. The recommendations will contribute towards encouraging community participation in the decision making within their municipalities. It is the aim of the study for other municipalities to use Buffalo City Municipality example as a benchmark for best practices. In conclusion, the rural areas of Buffalo City Metropolitan Municipality need more serious commitment from the municipality regarding sanitation and other basic services.
- Full Text:
- Authors: Marata, Mawethu Nicolas
- Date: 2012
- Subjects: Sanitation, Rural -- South Africa -- Eastern Cape , Sanitation -- South Africa -- Eastern Cape , Public utilities -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Buffalo City (South Africa)
- Language: English
- Type: Thesis , Masters , M Soc Sc (Rural Development)
- Identifier: vital:11944 , http://hdl.handle.net/10353/d1005966 , Sanitation, Rural -- South Africa -- Eastern Cape , Sanitation -- South Africa -- Eastern Cape , Public utilities -- South Africa -- Eastern Cape , Local government -- South Africa -- Eastern Cape , Buffalo City (South Africa)
- Description: This research paper is a qualitative evaluation of rural resident’s views in Buffalo City Metropolitan Municipality regarding the municipalities (BCM) performance in sanitation in rural areas. The study was conducted using interviews and some participant observation as a strategy for data collection. The data collected was analyzed using the basic needs theoretical framework. The study found that sanitation is the least talked about subject because of the stigma attached to it. The study found that Buffalo City Municipality initiatives in sanitation have little impact or has not yet transformed the lives of rural people. It has been found that the municipality is responding slowly in sanitation, and this situation impacts negatively on the lives of rural people as they are exposed to health hazards and diseases. The study questions the sustainability of toilets built in rural areas as many toilets have no roofs and door. It has been found that sanitation is still a challenge in the Buffalo City Municipality as some people still relieve themselves in the bushes. Some people even defecate into plastic bags and then throw the bags as far as they can. The study found that the environment is deeply affected by the lack of sanitation as both air and water are contaminated by waste disposal caused by poor sanitation. The general feeling of the residents of Buffalo City Municipality with regard to sanitation service delivery is that they were not happy at all. The residents are not happy with the state of sanitation services rendered by the municipality. The study calls upon the municipality to speedily address rural sanitation to avoid service delivery protest. The research calls upon organizations and companies to get sanitation issues as part of their function. The research made a number of recommendations in an effort to help municipalities accelerate the delivery of sanitation in rural areas. It is hoped that the recommendations would help the Buffalo City Municipality in ensuring that communities are equipped and empowered to meaningfully participate in making policies, regarding their development. The recommendations will contribute towards encouraging community participation in the decision making within their municipalities. It is the aim of the study for other municipalities to use Buffalo City Municipality example as a benchmark for best practices. In conclusion, the rural areas of Buffalo City Metropolitan Municipality need more serious commitment from the municipality regarding sanitation and other basic services.
- Full Text:
Drug abuse in selected Grahamstown schools
- Authors: Rungani, Judith
- Date: 2012
- Subjects: High school students -- Substance use -- South Africa -- Eastern Cape , School management and organization , Teenagers -- Drug abuse , Substance abuse -- Youth -- Grahamstown (South Africa)
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11759 , http://hdl.handle.net/10353/d1004784 , High school students -- Substance use -- South Africa -- Eastern Cape , School management and organization , Teenagers -- Drug abuse , Substance abuse -- Youth -- Grahamstown (South Africa)
- Description: The study explores drug abuse by learners in selected Grahamstown high schools. The study`s main concern is that drug abuse by learners is on the rise but yet there is less research on the drugs which are being used and the factors which motivate the learners to use such drugs. There are several drug abuse prevention strategies which are being implemented but yet they seem not to be very effective. It is in this frame of reference that this study saw it vital to focus on drug abuse by high school learners in Grahamstown. The study aims to identify the most commonly abused drugs by learners in the high schools of Grahamstown and the reasons why they use these drugs. The study made use of the mixed method research that is making use of both qualitative and quantitative research. The questionnaire was the instrument of data collection in quantitative data and interviews for the qualitative data. The packages which were used for the analysis of data include the Statistical Analysis System (SAS) V8 which was used to provide descriptive analysis and correlations. The findings of the study establish that the commonly abused drugs by learners are: alcohol with 58%, followed by cigarettes 22%, hookah-pipe 9%, dagga 7%. The main reasons why learners use drugs are: peer pressure, role models, availability, environment, and curiosity. Differences in drug use between male and female learners were noted. Twenty-five percent of the male learners and 16% of the female learners reported to be using drugs. In the view of these results, the researcher recommends that a comprehensive drug abuse prevention framework be formulated which focuses on preventing drug abuse at individual, family and community levels.
- Full Text:
- Authors: Rungani, Judith
- Date: 2012
- Subjects: High school students -- Substance use -- South Africa -- Eastern Cape , School management and organization , Teenagers -- Drug abuse , Substance abuse -- Youth -- Grahamstown (South Africa)
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11759 , http://hdl.handle.net/10353/d1004784 , High school students -- Substance use -- South Africa -- Eastern Cape , School management and organization , Teenagers -- Drug abuse , Substance abuse -- Youth -- Grahamstown (South Africa)
- Description: The study explores drug abuse by learners in selected Grahamstown high schools. The study`s main concern is that drug abuse by learners is on the rise but yet there is less research on the drugs which are being used and the factors which motivate the learners to use such drugs. There are several drug abuse prevention strategies which are being implemented but yet they seem not to be very effective. It is in this frame of reference that this study saw it vital to focus on drug abuse by high school learners in Grahamstown. The study aims to identify the most commonly abused drugs by learners in the high schools of Grahamstown and the reasons why they use these drugs. The study made use of the mixed method research that is making use of both qualitative and quantitative research. The questionnaire was the instrument of data collection in quantitative data and interviews for the qualitative data. The packages which were used for the analysis of data include the Statistical Analysis System (SAS) V8 which was used to provide descriptive analysis and correlations. The findings of the study establish that the commonly abused drugs by learners are: alcohol with 58%, followed by cigarettes 22%, hookah-pipe 9%, dagga 7%. The main reasons why learners use drugs are: peer pressure, role models, availability, environment, and curiosity. Differences in drug use between male and female learners were noted. Twenty-five percent of the male learners and 16% of the female learners reported to be using drugs. In the view of these results, the researcher recommends that a comprehensive drug abuse prevention framework be formulated which focuses on preventing drug abuse at individual, family and community levels.
- Full Text:
Participation and paradoxes: community control of mineral wealth in South Africa's Royal Bafokeng and Bakgatla Ba Kgafela communities
- Authors: Mnwana, Sonwabile Comfords
- Date: 2012
- Subjects: Political participation -- South Africa , Natural resources -- Co-management -- South Africa , Rural development -- South Africa , Mining corporations -- South Africa , Platinum mines and mining -- South Africa , Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Social Sciences)
- Identifier: vital:11952 , http://hdl.handle.net/10353/526 , Political participation -- South Africa , Natural resources -- Co-management -- South Africa , Rural development -- South Africa , Mining corporations -- South Africa , Platinum mines and mining -- South Africa , Sustainable development -- South Africa
- Description: Resource control as a form of community participation in the mineral economy has gained much recognition. One prevailing argument is that direct control of natural resources by local communities is an important precondition for equitable utilisation of the natural resource wealth, peaceful co-existence between mining corporations and indigenous communities, and congenial relations between local communities and the state. Studies have also shown that the absence of direct community control of mineral wealth remains a major factor in the communal resistance and socio-political conflict witnessed in the natural resource-endowed regions of countries such as Nigeria, Ecuador, Sierra Leone and the Democratic Republic of the Congo. However, little is known about mineral resource control at the community level. Does community control necessarily translate to equity? How does local involvement in the mobilisation of mining royalties benefit different segments of the community? Indeed, how do different segments of the community “control” the wealth? What is the specific model adopted to engender broad-based community participation in the utilisation of mineral wealth – and does it matter? These theoretical and practical questions were the impetus for undertaking this study in the Royal Bafokeng and Bakgatla Ba Kgafela – two platinum-rich ‘traditional’ communities in South Africa’s North West Province that have significant control over platinum resources in their territories. Utilising ethnographic data collected in the two study communities in 2008 and 2009, the thesis examines the character of community participation in platinum wealth utilisation; specifically, the conditions under which community participation promotes or hinders sustainable community development. The analysis uses a “three-dimensional participation ladder” conceptual scheme, based in part on Sherry Arnstein’s (1969) “ladder of citizen participation” and subsequent typologies of participation. Among the key findings of the thesis are that despite observed benefits, the interface of resource wealth and community development is fraught with tokenistic participation, elite-targeted grassroots anger, and local tensions – all linked to the contradictory nature of participation. The thesis further reveals that in some instances the challenge of platinum wealth-engendered community development tends to undermine existing customary and democratic spaces for participation, and that this is exacerbated by community-level issues such as poverty and inequality. The findings of the study compel a shift of analytical focus from conflict as an epiphenomenon of collective community exclusion and deprivation (as in the case of many natural-rich countries in Sub-Saharan Africa and elsewhere), to conflict as also resulting from collective community inclusion (in natural resource utilisation). At the policy level, the study generates insights that will, hopefully, assist mineral resource-endowed countries, such as South Africa, in dealing with the challenge of developing appropriate policy frameworks for regulating business and social partnerships between local communities and mining corporations, and within resource-rich communities themselves.
- Full Text:
- Authors: Mnwana, Sonwabile Comfords
- Date: 2012
- Subjects: Political participation -- South Africa , Natural resources -- Co-management -- South Africa , Rural development -- South Africa , Mining corporations -- South Africa , Platinum mines and mining -- South Africa , Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Social Sciences)
- Identifier: vital:11952 , http://hdl.handle.net/10353/526 , Political participation -- South Africa , Natural resources -- Co-management -- South Africa , Rural development -- South Africa , Mining corporations -- South Africa , Platinum mines and mining -- South Africa , Sustainable development -- South Africa
- Description: Resource control as a form of community participation in the mineral economy has gained much recognition. One prevailing argument is that direct control of natural resources by local communities is an important precondition for equitable utilisation of the natural resource wealth, peaceful co-existence between mining corporations and indigenous communities, and congenial relations between local communities and the state. Studies have also shown that the absence of direct community control of mineral wealth remains a major factor in the communal resistance and socio-political conflict witnessed in the natural resource-endowed regions of countries such as Nigeria, Ecuador, Sierra Leone and the Democratic Republic of the Congo. However, little is known about mineral resource control at the community level. Does community control necessarily translate to equity? How does local involvement in the mobilisation of mining royalties benefit different segments of the community? Indeed, how do different segments of the community “control” the wealth? What is the specific model adopted to engender broad-based community participation in the utilisation of mineral wealth – and does it matter? These theoretical and practical questions were the impetus for undertaking this study in the Royal Bafokeng and Bakgatla Ba Kgafela – two platinum-rich ‘traditional’ communities in South Africa’s North West Province that have significant control over platinum resources in their territories. Utilising ethnographic data collected in the two study communities in 2008 and 2009, the thesis examines the character of community participation in platinum wealth utilisation; specifically, the conditions under which community participation promotes or hinders sustainable community development. The analysis uses a “three-dimensional participation ladder” conceptual scheme, based in part on Sherry Arnstein’s (1969) “ladder of citizen participation” and subsequent typologies of participation. Among the key findings of the thesis are that despite observed benefits, the interface of resource wealth and community development is fraught with tokenistic participation, elite-targeted grassroots anger, and local tensions – all linked to the contradictory nature of participation. The thesis further reveals that in some instances the challenge of platinum wealth-engendered community development tends to undermine existing customary and democratic spaces for participation, and that this is exacerbated by community-level issues such as poverty and inequality. The findings of the study compel a shift of analytical focus from conflict as an epiphenomenon of collective community exclusion and deprivation (as in the case of many natural-rich countries in Sub-Saharan Africa and elsewhere), to conflict as also resulting from collective community inclusion (in natural resource utilisation). At the policy level, the study generates insights that will, hopefully, assist mineral resource-endowed countries, such as South Africa, in dealing with the challenge of developing appropriate policy frameworks for regulating business and social partnerships between local communities and mining corporations, and within resource-rich communities themselves.
- Full Text:
Legal status and protection of animals in South Africa
- Authors: Hartwig, Wendy
- Date: 2012
- Subjects: Human-animal relationships -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11118 , http://hdl.handle.net/10353/515
- Description: The animal welfare legislation that is discussed in this Dissertation is just a sample of the available legislation from the chosen foreign jurisdictions and South Africa. The chosen foreign jurisdictions were chosen as a lens to gain a needed perspective on South African animal welfare legislation. The legislation chosen for discussion falls within particular categories that are discussed fully in the later chapters.i Despite the fact that the animal rights and animal welfare movements are recorded to date back as far as 500B.C, the majority of jurisdictions throughout the world still consider animals to be property that can be bought, traded, hunted and after they are killed, their remains kept as trophies or souvenirs. Within these jurisdictions (which includes South Africa and the other four chosen foreign jurisdictions – Kenya, India, Switzerland and the United States of America) there is a demonstrated lack of proper enforcement of the animal welfare/animal anti-cruelty legislation, regulations and industry rules, which is made worse by the actions of uncaring, abusive and/or ignorant people. South Africa is no better or worse to the four chosen jurisdictions in that it has similar anti-cruelty/animal welfare legislation. The lack of proper enforcement of this animal welfare legislation in South Africa should be of great concern as many studies have indicated that there is a link between animal abuse/cruelty and ‘human’ abuse. The same studies also indicate that animal abusers are at a greater risk of becoming violent criminals or of committing a violent crime. For example, the Federal Bureau of Investigation has noted that most serial killers in the USA had a history of torturing, abusing and killing animals before they moved on to torturing, abusing and/or killing humans in their adult life. Needed changes to the animal welfare legislation and how people view animals should be made in South Africa to ensure that welfare of animals is protected. For example, the Government could educate people about animal welfare in order to overcome any ignorance that may be the cause of animal pain and abuse, as well as strengthening existing animal welfare legislation. The eradication of ignorance, as well as a necessary change in the current animal welfare legislation, will help to create a real change in how people view and treat i Chapter 5 and 6. [iii] animals. People will come to realise that animals exist in their own right and that they were not created to serve or to be exploited by man.
- Full Text:
- Authors: Hartwig, Wendy
- Date: 2012
- Subjects: Human-animal relationships -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11118 , http://hdl.handle.net/10353/515
- Description: The animal welfare legislation that is discussed in this Dissertation is just a sample of the available legislation from the chosen foreign jurisdictions and South Africa. The chosen foreign jurisdictions were chosen as a lens to gain a needed perspective on South African animal welfare legislation. The legislation chosen for discussion falls within particular categories that are discussed fully in the later chapters.i Despite the fact that the animal rights and animal welfare movements are recorded to date back as far as 500B.C, the majority of jurisdictions throughout the world still consider animals to be property that can be bought, traded, hunted and after they are killed, their remains kept as trophies or souvenirs. Within these jurisdictions (which includes South Africa and the other four chosen foreign jurisdictions – Kenya, India, Switzerland and the United States of America) there is a demonstrated lack of proper enforcement of the animal welfare/animal anti-cruelty legislation, regulations and industry rules, which is made worse by the actions of uncaring, abusive and/or ignorant people. South Africa is no better or worse to the four chosen jurisdictions in that it has similar anti-cruelty/animal welfare legislation. The lack of proper enforcement of this animal welfare legislation in South Africa should be of great concern as many studies have indicated that there is a link between animal abuse/cruelty and ‘human’ abuse. The same studies also indicate that animal abusers are at a greater risk of becoming violent criminals or of committing a violent crime. For example, the Federal Bureau of Investigation has noted that most serial killers in the USA had a history of torturing, abusing and killing animals before they moved on to torturing, abusing and/or killing humans in their adult life. Needed changes to the animal welfare legislation and how people view animals should be made in South Africa to ensure that welfare of animals is protected. For example, the Government could educate people about animal welfare in order to overcome any ignorance that may be the cause of animal pain and abuse, as well as strengthening existing animal welfare legislation. The eradication of ignorance, as well as a necessary change in the current animal welfare legislation, will help to create a real change in how people view and treat i Chapter 5 and 6. [iii] animals. People will come to realise that animals exist in their own right and that they were not created to serve or to be exploited by man.
- Full Text:
Exploring the influence of mindfulness-based stress reduction (MBSR) programmes on participants experience of time, particularly the present (here and now): a case study of Eastern Cape participants
- Authors: Schofield, Lorna
- Date: 2012
- Subjects: Acceptance and commitment therapy , Stress management , Depression, Mental -- Treatment
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11611 , http://hdl.handle.net/10353/d1005640
- Description: This study aims to explore the extent to which participating in a Mindfulness-Based Stress Reduction (MBSR) programme may result in shifts in people’s relationship with time, notably whether they become more present-focussed. The eight week MBSR programme advocates mindfulness, which is defined as paying attention on purpose in the present moment without judgement, as a way of reducing stress. The programme has been available in East London since 2009. A case study of eight MBSR programme participants’ experiences using narrative analysis was conducted. Narrative psychology and social constructionism provided the theoretical basis in which our storied lives are located in culturally inscribed narratives, with specific discourses around time and stress. Time discourses tend to pressurise people to believe that it is better to go about daily life at a fast pace, which requires significant hurrying and rushing with pervasive senses of time urgency. Stress discourse locates stress management within individuals. One-on-one semi structured interviews were held so that participants could reflect on their experience of time and the present moment orientation of the programme. Participants’ perceived a shift in how they experienced time with greater awareness of being present-focussed and they identified stress reduction benefits, which included feeling calmer, less panicked and more self-accepting. However, some of the participants maintaining the formal mindfulness practices like the body scan, meditation and mindful movement after the programme often proved difficult, as they were drawn back into their dominant narratives around time which were characterised by busyness, productivity and time scarcity.
- Full Text:
- Authors: Schofield, Lorna
- Date: 2012
- Subjects: Acceptance and commitment therapy , Stress management , Depression, Mental -- Treatment
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11611 , http://hdl.handle.net/10353/d1005640
- Description: This study aims to explore the extent to which participating in a Mindfulness-Based Stress Reduction (MBSR) programme may result in shifts in people’s relationship with time, notably whether they become more present-focussed. The eight week MBSR programme advocates mindfulness, which is defined as paying attention on purpose in the present moment without judgement, as a way of reducing stress. The programme has been available in East London since 2009. A case study of eight MBSR programme participants’ experiences using narrative analysis was conducted. Narrative psychology and social constructionism provided the theoretical basis in which our storied lives are located in culturally inscribed narratives, with specific discourses around time and stress. Time discourses tend to pressurise people to believe that it is better to go about daily life at a fast pace, which requires significant hurrying and rushing with pervasive senses of time urgency. Stress discourse locates stress management within individuals. One-on-one semi structured interviews were held so that participants could reflect on their experience of time and the present moment orientation of the programme. Participants’ perceived a shift in how they experienced time with greater awareness of being present-focussed and they identified stress reduction benefits, which included feeling calmer, less panicked and more self-accepting. However, some of the participants maintaining the formal mindfulness practices like the body scan, meditation and mindful movement after the programme often proved difficult, as they were drawn back into their dominant narratives around time which were characterised by busyness, productivity and time scarcity.
- Full Text:
Commensal bacteria belonging to the Staphylococcus Acinetobacter and Stenotrophomonas genera as reservoirs of antibiotic resistance determinants in the environment of Nkonkobe Municipality, Eastern Cape Province , South Africa
- Authors: Adegoke, Anthony Ayodeji
- Date: 2012
- Subjects: Acinetobacter infections , Drug resistance in microorganisms , Staphylococcal infections , Bacterial diseases
- Language: English
- Type: Thesis , Doctoral , Degree
- Identifier: http://hdl.handle.net/10353/6539 , vital:30551
- Description: A study to assess the potentials of some commensal bacteria that belong to Staphylococcus, Acinetobacter and Stenotrophomonas genera as reservoirs of antibiotic resistance determinants in the environment of Nkonkobe Municipality of the Eastern Cape Province, South Africa, was carried out using standard microbiological and molecular techniques. A total of 120 Staphylococcus isolates which consisted of Staphylococcus haemolyticus (30%), Staphylococcus aureus (23.3%) from pig; Staphylococcus capitis (15%) from goat; Staphylococcus heamolyticus (5%) and Staphylococcus xylosus (15%) from cattle and other Staphylococci (11%) from dead chicken and pigs were isolated. About 23.3% of these isolates were coagulase positive and 76.7% were coagulase negative. This difference in prevalence along coagulase production divide was statistically significant (p < 0.05). Eighty-six Acinetobacter species (Acinetobacter baumannii/calcoaceticus and Acinetobacter haemolyticus) were also isolated from Alice and Fort Beaufort towns samples, while 125 Stenotrophomonas maltophilia isolates were from grass root rhizosphere (96%) and soil butternut root rhizosphere (4%). Between 75-100% of the Staphylococccus species were resistant to Penicillin G, tetracycline, sulphamethaxole and nalidixic acid; about 38 % were methicillin resistant, consisting of 12.6% methicillin resistant Staphylococcus aureus (MRSA) from pig and a total of 12% vancomycin resistant were observed. Also, 12% of the isolates were erythromycin resistant while 40.2 % were resistant to the third generation cephalosporin, ceftazidime. The antibiotic resistance genes vanA, VanB, eryA, eryB, eryC were not detected in all the phenotypically resistant Staphylococccus species, but mec A gene and mph genes were detected. In the Acinetobacter species, a wide range of 30-100% resistance to penicillin G, ceftriazone, nitrofurantoin, erythromycin, and augmentin was observed. Polymerase chain reaction (PCR) revealed the presence of Tet(B) and Tet(39) genes in these species, while Tet (A), Tet(M) and Tet(H) were absent. Also, 9.3% of the Acinetobacter species showed phenotypic production of extended spectrum beta lactamases (ESBLs) while 3.5% were positive for the presence of blaCTX-M-1 genes. The Stenotrophomonas maltophilia isolates showed varying resistance to meropenem (8.9%), cefuroxime (95.6 %), ampicillin-sulbactam (53.9%), ceftazidime (10.7%), cefepime (29.3 %), minocycline (2.2%), kanamycin (56.9%), ofloxacin (2.9%), levofloxacin (1.3%), moxifloxacin (2.8%), ciprofloxacin (24.3%), gatifloxacin (1.3%), polymyxin B (2.9 %), cotrimoxazole (26.1%), trimethoprim (98.6%), aztreonam(58%) and Polymyxin B (2.9 %). The isolates exhibited significant susceptibility to the fluoroquinolones (74.3-94.7 %), polymycin (97.1%) and meropenem (88.1%). Only sul3 genes were the only sulphonamide resistance gene detected among the trimethoprim-sulphamethoxazole resistant isolates. The observed multiple antibiotic resistance indeces (MARI) of >2 for Staphylococcus species, Acinetobacter species and Stenotrophomonas maltophilia suggest that they have arisen from high-risk sources where antibiotics are in constant arbitrary use resulting in high selective pressure. The presence of tetracycline resistance genes in Acinetobacter species justifies the observed phenotypic resistance to oxytetracycline and intermediate resistance to minocycline. High phenotypic resistance and the presence of some resistance genes in Staphylococcus species is a possible threat to public health and suggests animals to be important reservoirs of antibiotic resistance determinants in the environment. Indiscriminate use of antibiotics induces this kind of antibiotic resistance and should be discouraged. Personal hygiene is encouraged as it reduces the load of Acinetobacter species contacted from the environment that may be difficult to control. Commensal Stenotrophomonas maltophilia are as important as their clinical counterparts due to their roles in opportunistic infection, antibiotic resistance and their associated genes, especially sul gene. Personal hygiene is hereby advocated especially when in contact with soil, plants and plants’ rhizospheric soil.
- Full Text:
- Authors: Adegoke, Anthony Ayodeji
- Date: 2012
- Subjects: Acinetobacter infections , Drug resistance in microorganisms , Staphylococcal infections , Bacterial diseases
- Language: English
- Type: Thesis , Doctoral , Degree
- Identifier: http://hdl.handle.net/10353/6539 , vital:30551
- Description: A study to assess the potentials of some commensal bacteria that belong to Staphylococcus, Acinetobacter and Stenotrophomonas genera as reservoirs of antibiotic resistance determinants in the environment of Nkonkobe Municipality of the Eastern Cape Province, South Africa, was carried out using standard microbiological and molecular techniques. A total of 120 Staphylococcus isolates which consisted of Staphylococcus haemolyticus (30%), Staphylococcus aureus (23.3%) from pig; Staphylococcus capitis (15%) from goat; Staphylococcus heamolyticus (5%) and Staphylococcus xylosus (15%) from cattle and other Staphylococci (11%) from dead chicken and pigs were isolated. About 23.3% of these isolates were coagulase positive and 76.7% were coagulase negative. This difference in prevalence along coagulase production divide was statistically significant (p < 0.05). Eighty-six Acinetobacter species (Acinetobacter baumannii/calcoaceticus and Acinetobacter haemolyticus) were also isolated from Alice and Fort Beaufort towns samples, while 125 Stenotrophomonas maltophilia isolates were from grass root rhizosphere (96%) and soil butternut root rhizosphere (4%). Between 75-100% of the Staphylococccus species were resistant to Penicillin G, tetracycline, sulphamethaxole and nalidixic acid; about 38 % were methicillin resistant, consisting of 12.6% methicillin resistant Staphylococcus aureus (MRSA) from pig and a total of 12% vancomycin resistant were observed. Also, 12% of the isolates were erythromycin resistant while 40.2 % were resistant to the third generation cephalosporin, ceftazidime. The antibiotic resistance genes vanA, VanB, eryA, eryB, eryC were not detected in all the phenotypically resistant Staphylococccus species, but mec A gene and mph genes were detected. In the Acinetobacter species, a wide range of 30-100% resistance to penicillin G, ceftriazone, nitrofurantoin, erythromycin, and augmentin was observed. Polymerase chain reaction (PCR) revealed the presence of Tet(B) and Tet(39) genes in these species, while Tet (A), Tet(M) and Tet(H) were absent. Also, 9.3% of the Acinetobacter species showed phenotypic production of extended spectrum beta lactamases (ESBLs) while 3.5% were positive for the presence of blaCTX-M-1 genes. The Stenotrophomonas maltophilia isolates showed varying resistance to meropenem (8.9%), cefuroxime (95.6 %), ampicillin-sulbactam (53.9%), ceftazidime (10.7%), cefepime (29.3 %), minocycline (2.2%), kanamycin (56.9%), ofloxacin (2.9%), levofloxacin (1.3%), moxifloxacin (2.8%), ciprofloxacin (24.3%), gatifloxacin (1.3%), polymyxin B (2.9 %), cotrimoxazole (26.1%), trimethoprim (98.6%), aztreonam(58%) and Polymyxin B (2.9 %). The isolates exhibited significant susceptibility to the fluoroquinolones (74.3-94.7 %), polymycin (97.1%) and meropenem (88.1%). Only sul3 genes were the only sulphonamide resistance gene detected among the trimethoprim-sulphamethoxazole resistant isolates. The observed multiple antibiotic resistance indeces (MARI) of >2 for Staphylococcus species, Acinetobacter species and Stenotrophomonas maltophilia suggest that they have arisen from high-risk sources where antibiotics are in constant arbitrary use resulting in high selective pressure. The presence of tetracycline resistance genes in Acinetobacter species justifies the observed phenotypic resistance to oxytetracycline and intermediate resistance to minocycline. High phenotypic resistance and the presence of some resistance genes in Staphylococcus species is a possible threat to public health and suggests animals to be important reservoirs of antibiotic resistance determinants in the environment. Indiscriminate use of antibiotics induces this kind of antibiotic resistance and should be discouraged. Personal hygiene is encouraged as it reduces the load of Acinetobacter species contacted from the environment that may be difficult to control. Commensal Stenotrophomonas maltophilia are as important as their clinical counterparts due to their roles in opportunistic infection, antibiotic resistance and their associated genes, especially sul gene. Personal hygiene is hereby advocated especially when in contact with soil, plants and plants’ rhizospheric soil.
- Full Text: