Effect of post-slaughter handling on physico-chemical and microbiological quality of red meat along the distribution chain in the Eastern Cape Province, South Africa
- Authors: Rani, Zikhona Theodora
- Date: 2015
- Subjects: Meat -- Microbiology Meat -- Transportation -- South Africa -- Eastern Cape Meat -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2490 , vital:27875
- Description: The broad objective of the study was to investigate the effect of post-slaughter handling in the distribution chain on red meat quality and safety. A survey was conducted among 300 consumers and 100 meat handlers in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi) in the Eastern Cape Province of South Africa to investigate their perceptions on meat quality and safety, together with challenges faced by meat handlers during the distribution of meat from the abattoir to retailers. The microbiological profile and physico-chemical quality of red meat at different stages of the abattoir to retail outlets in the distribution chain were also determined. Swabs (n=216) and meat samples (n=450) were collected from beef, pork and mutton carcasses during the loading process of carcasses into trucks at the abattoir, when offloading carcasses at the supply points and during marketing. Physico-chemical qualities such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at each point. To determine the microbiological profiles of the carcasses, four microbiological parameters were considered: Total bacteria count (general bacteria), coliform count (related to hygiene and indicator for pathogens), Escherichia coli (Gram-negative pathogen) and Staphylococcus aureus (Gram-positive pathogen). Two types of packaging (vacuum and overwrapping) were used to determine their effect on shelf-life and microbiological quality of red meat under the normal marketing conditions over a storage duration of 15 days. The results from the study showed low awareness of consumers about the pathogenic diseases which arise from meat. A strong significant association (p ˂ 0.05) between educational status and awareness on meat safety was observed. Most of the consumers perceived that quality goes beyond safety such that 35.6 percent of the respondents indicated that they did not have a problem with consuming spoiled meat, whilst the remaining 64.4 percent indicated that they would r eject spoiled meat. Although retailers indicated that they take meat safety into consideration in their shops, 92 percent of the retailers revealed that they do not perform microbial assessment of meat in their shops. A series of loading and off-loading, temperature fluctuations, environmental temperatures and ques during offloading were reported as the major challenges during transportation of carcasses from the abattoir to the supply points. The microbial counts were significantly (p<0.05) higher in samples from the commercial abattoir than in those from the communal abattoir. Escherichia coli was the predominant microbial contaminant in the samples from both abattoirs. When following the chain, total bacterial count (TBC), coliform count (CC) and the levels of E. coli contamination increased progressively between the loading and the off-loading points (5.1 to 7.9 log10 CFU/cm2; 5.0 to 5.6 log10 CFU/cm2 and 2.7 to 3.7 log10 CFU/cm2, respectively). The storage period, meat type, distance during transportation and temperature were found to have a significant impact on the microbial levels during the distribution of carcasses. Distribution stage had a significant effect (p<0.05) on some of the physico- chemical meat quality attributes and differences in the lightness (L*) and redness (a*) values between the loading, off-loading and display points were observed. Consumers perceived retailer class as one of the factors influencing meat quality, but according to the instrumental measurements retailer class did not have a significant effect on physico-chemical meat quality. However, distance and storage duration significantly (p<0.05) affected (L*) and (a*) values in the meat during distribution chain. Vacuum and overwrapping packaging significantly affected (p<0.05) the shelf life of meat. Therefore, it was concluded that post-slaughter handling during the distribution chain affects the physico- chemical, microbiological and shelf-life of meat.
- Full Text:
- Date Issued: 2015
- Authors: Rani, Zikhona Theodora
- Date: 2015
- Subjects: Meat -- Microbiology Meat -- Transportation -- South Africa -- Eastern Cape Meat -- Quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2490 , vital:27875
- Description: The broad objective of the study was to investigate the effect of post-slaughter handling in the distribution chain on red meat quality and safety. A survey was conducted among 300 consumers and 100 meat handlers in five different municipalities (Buffalo City, Nkonkobe, Ngqushwa, Lukhanje and Amahlathi) in the Eastern Cape Province of South Africa to investigate their perceptions on meat quality and safety, together with challenges faced by meat handlers during the distribution of meat from the abattoir to retailers. The microbiological profile and physico-chemical quality of red meat at different stages of the abattoir to retail outlets in the distribution chain were also determined. Swabs (n=216) and meat samples (n=450) were collected from beef, pork and mutton carcasses during the loading process of carcasses into trucks at the abattoir, when offloading carcasses at the supply points and during marketing. Physico-chemical qualities such as colour (L* - lightness, b* - redness and a* - yellowness) and meat pH measurements were taken at each point. To determine the microbiological profiles of the carcasses, four microbiological parameters were considered: Total bacteria count (general bacteria), coliform count (related to hygiene and indicator for pathogens), Escherichia coli (Gram-negative pathogen) and Staphylococcus aureus (Gram-positive pathogen). Two types of packaging (vacuum and overwrapping) were used to determine their effect on shelf-life and microbiological quality of red meat under the normal marketing conditions over a storage duration of 15 days. The results from the study showed low awareness of consumers about the pathogenic diseases which arise from meat. A strong significant association (p ˂ 0.05) between educational status and awareness on meat safety was observed. Most of the consumers perceived that quality goes beyond safety such that 35.6 percent of the respondents indicated that they did not have a problem with consuming spoiled meat, whilst the remaining 64.4 percent indicated that they would r eject spoiled meat. Although retailers indicated that they take meat safety into consideration in their shops, 92 percent of the retailers revealed that they do not perform microbial assessment of meat in their shops. A series of loading and off-loading, temperature fluctuations, environmental temperatures and ques during offloading were reported as the major challenges during transportation of carcasses from the abattoir to the supply points. The microbial counts were significantly (p<0.05) higher in samples from the commercial abattoir than in those from the communal abattoir. Escherichia coli was the predominant microbial contaminant in the samples from both abattoirs. When following the chain, total bacterial count (TBC), coliform count (CC) and the levels of E. coli contamination increased progressively between the loading and the off-loading points (5.1 to 7.9 log10 CFU/cm2; 5.0 to 5.6 log10 CFU/cm2 and 2.7 to 3.7 log10 CFU/cm2, respectively). The storage period, meat type, distance during transportation and temperature were found to have a significant impact on the microbial levels during the distribution of carcasses. Distribution stage had a significant effect (p<0.05) on some of the physico- chemical meat quality attributes and differences in the lightness (L*) and redness (a*) values between the loading, off-loading and display points were observed. Consumers perceived retailer class as one of the factors influencing meat quality, but according to the instrumental measurements retailer class did not have a significant effect on physico-chemical meat quality. However, distance and storage duration significantly (p<0.05) affected (L*) and (a*) values in the meat during distribution chain. Vacuum and overwrapping packaging significantly affected (p<0.05) the shelf life of meat. Therefore, it was concluded that post-slaughter handling during the distribution chain affects the physico- chemical, microbiological and shelf-life of meat.
- Full Text:
- Date Issued: 2015
Effective and efficient performance management in local government, with reference to the Cacadu District Municipality
- Authors: Asmah-Andoh, Kwame
- Date: 2012
- Subjects: Performance -- Management , Performance standards -- South Africa -- Eastern Cape , Municipal government -- South Africa -- Eastern Cape , Employees -- Rating of -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8161 , http://hdl.handle.net/10948/1633 , Performance -- Management , Performance standards -- South Africa -- Eastern Cape , Municipal government -- South Africa -- Eastern Cape , Employees -- Rating of -- South Africa
- Description: This study examined the existing levels of effectiveness and efficiency in local government with the application of performance management systems in the provision of municipal services. The provision of district-wide water services in Cacadu District Municipality in the Province of the Eastern Cape, South Africa is used for particular reference. Local government reforms after 1994 led to the creation of the district municipality (which groups together a number of local municipalities) to drive district-wide services provision, the introduction of systematic services provision standards and performance management as part of management reforms in municipalities. The problem for the research deals with how the Cacadu District Council and the councils of the local municipalities within the district are collaborating in using performance management systems, what effects this could have on programmes and services provision and how communication of performance information would improve service provision. Conceptual and practical difficulties encountered in effective and efficient utilisation and some obstacles that contribute to impeding progress in the use of performance management systems in municipalities were analysed. The qualitative research included a review of documents from the public administration and management literature; discourse analysis of interviews with municipal councilors and appointed officials, community members and provincial managers. The document review included a review of municipal documents that incorporated performance management or described development and use of performance management systems. It also included the legal frameworks and statutory requirements for municipal government and administration. All the responding municipal councils within the Cacadu District Municipality reported using performance management over the past five years yet little empirical evidence explains usage for district-wide programmes and services provision. Research shows that a comprehensive performance management approach to programmes and services has a more effective and efficient impact on service improvement than utilisation of personnnel appraisal (Ammons and Rivenbark, 2005). Despite the expected benefits, personnel performance appraisal is often flawed and the need exists in the district municipal model to develop and utilise performance management systems that validly assess the work performance of personnel (Hindo, 2010). Performance management system utilisation in decision making management is more related to the long-term benefits of effectiveness and efficiency than personnel appraisal for performance rewards and reporting. To overcome and address a situation of managing performance without a performance management system the study proposes a programme performance measurement and management system which incorporates information on management inputs, programme-specific data and citizens' feedback for outcomes and impact of the service. The suggested design for district-wide and specific municipal services provision elicit and provide relevant and appropriate performance information for management, decision-making and accountability to community. The effectiveness of the proposed system is to provide information for decision-making, long-term planning, strategic management and accountability reporting. The proposal is built on the exisiting method and thus efficient in the utilisation of resources. This has implications both for the theory of Public Administration and Management and the practical application of performamce management in public institutions. The study explicity provides a Public Administration perspective on management in a multi-municipal district context with different authorities. The study contributes to the theory of administrative policy and management context of the municipality with special attention to functions for which different authorities are responsible and the role of a performance system for better insught into managing overlapping authority, gaps and duplications. A central purpose of public management research is to theorise and influence practice. The practical utilisation of the proposed method is intended to assist managers and politicians with better understanding of a sustainable performance management system.
- Full Text:
- Date Issued: 2012
- Authors: Asmah-Andoh, Kwame
- Date: 2012
- Subjects: Performance -- Management , Performance standards -- South Africa -- Eastern Cape , Municipal government -- South Africa -- Eastern Cape , Employees -- Rating of -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8161 , http://hdl.handle.net/10948/1633 , Performance -- Management , Performance standards -- South Africa -- Eastern Cape , Municipal government -- South Africa -- Eastern Cape , Employees -- Rating of -- South Africa
- Description: This study examined the existing levels of effectiveness and efficiency in local government with the application of performance management systems in the provision of municipal services. The provision of district-wide water services in Cacadu District Municipality in the Province of the Eastern Cape, South Africa is used for particular reference. Local government reforms after 1994 led to the creation of the district municipality (which groups together a number of local municipalities) to drive district-wide services provision, the introduction of systematic services provision standards and performance management as part of management reforms in municipalities. The problem for the research deals with how the Cacadu District Council and the councils of the local municipalities within the district are collaborating in using performance management systems, what effects this could have on programmes and services provision and how communication of performance information would improve service provision. Conceptual and practical difficulties encountered in effective and efficient utilisation and some obstacles that contribute to impeding progress in the use of performance management systems in municipalities were analysed. The qualitative research included a review of documents from the public administration and management literature; discourse analysis of interviews with municipal councilors and appointed officials, community members and provincial managers. The document review included a review of municipal documents that incorporated performance management or described development and use of performance management systems. It also included the legal frameworks and statutory requirements for municipal government and administration. All the responding municipal councils within the Cacadu District Municipality reported using performance management over the past five years yet little empirical evidence explains usage for district-wide programmes and services provision. Research shows that a comprehensive performance management approach to programmes and services has a more effective and efficient impact on service improvement than utilisation of personnnel appraisal (Ammons and Rivenbark, 2005). Despite the expected benefits, personnel performance appraisal is often flawed and the need exists in the district municipal model to develop and utilise performance management systems that validly assess the work performance of personnel (Hindo, 2010). Performance management system utilisation in decision making management is more related to the long-term benefits of effectiveness and efficiency than personnel appraisal for performance rewards and reporting. To overcome and address a situation of managing performance without a performance management system the study proposes a programme performance measurement and management system which incorporates information on management inputs, programme-specific data and citizens' feedback for outcomes and impact of the service. The suggested design for district-wide and specific municipal services provision elicit and provide relevant and appropriate performance information for management, decision-making and accountability to community. The effectiveness of the proposed system is to provide information for decision-making, long-term planning, strategic management and accountability reporting. The proposal is built on the exisiting method and thus efficient in the utilisation of resources. This has implications both for the theory of Public Administration and Management and the practical application of performamce management in public institutions. The study explicity provides a Public Administration perspective on management in a multi-municipal district context with different authorities. The study contributes to the theory of administrative policy and management context of the municipality with special attention to functions for which different authorities are responsible and the role of a performance system for better insught into managing overlapping authority, gaps and duplications. A central purpose of public management research is to theorise and influence practice. The practical utilisation of the proposed method is intended to assist managers and politicians with better understanding of a sustainable performance management system.
- Full Text:
- Date Issued: 2012
Effects of 99-year leases on newly resettled farmers’ farm improvement, productivity and empowerment in Zimbabwe, 2007 to 2013
- Authors: Makaye, Peter
- Date: 2019
- Subjects: Land reform - -Zimbabwe , Land tenure -- Zimbabwe Farmers -- Zimbabwe Sustainable development -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/40812 , vital:36239
- Description: This study seeks to interrogate the effects of 99-year leases on newly resettled farmers, with focus on Zimbabwe’s Fast Track Land Reform programme, shifting from its causes and processes to its variegated outcomes., The focus was on how the leasehold system has impinged on three variables: farm improvement, productivity, and empowerment. Quantitative data was collected from 92 newly resettled farmers randomly selected from three different farming regions in Zimbabwe: Region 2, an agriculturally well-endowed area concentrating on crop production, Region 4, an arid and hot region concentrating on livestock, and Region 5, specialising in sugar cane production under irrigation. To corroborate the survey findings, follow-up interviews and focus group discussions (FGDs) were carried out in these regions. Interviews with purposively sampled experts in agriculture in Zimbabwe were also conducted. Data are presented in charts and graphs as well as detailed descriptions. The study found that the 99-year leases influenced the fortunes of the farmers in different ways. In some areas, depending on the crop grown, the 99-year leases were accepted as a basis for a loan. In other instances, the leases have not been accepted as collateral and this has constrained the farmers’ ability to optimally utilise their farms optimally. Despite a general sense of insecurity, the farmers have shown a willingness to re-invest in the farms., Many improvements such as new accommodation for employees, the erection of fire guards, and the development of water sources are being made on the farms, all pointing to a desire for sustained utilisation of the A2 farms. The researcher proposes that serious effort has to be made towards making the 99-year leases acceptable as collateral for loans because otherwise, in the context of general undercapitalisation among the farmers, their ability to make the medium-scale farms optimally contribute to national accumulation will remain constrained. The form of tenure is related to the empowerment of the farmers, both materially and socially. This is in turn related to the sustainable use of the land and hence to sustainable development in Zimbabwe.
- Full Text:
- Date Issued: 2019
- Authors: Makaye, Peter
- Date: 2019
- Subjects: Land reform - -Zimbabwe , Land tenure -- Zimbabwe Farmers -- Zimbabwe Sustainable development -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/40812 , vital:36239
- Description: This study seeks to interrogate the effects of 99-year leases on newly resettled farmers, with focus on Zimbabwe’s Fast Track Land Reform programme, shifting from its causes and processes to its variegated outcomes., The focus was on how the leasehold system has impinged on three variables: farm improvement, productivity, and empowerment. Quantitative data was collected from 92 newly resettled farmers randomly selected from three different farming regions in Zimbabwe: Region 2, an agriculturally well-endowed area concentrating on crop production, Region 4, an arid and hot region concentrating on livestock, and Region 5, specialising in sugar cane production under irrigation. To corroborate the survey findings, follow-up interviews and focus group discussions (FGDs) were carried out in these regions. Interviews with purposively sampled experts in agriculture in Zimbabwe were also conducted. Data are presented in charts and graphs as well as detailed descriptions. The study found that the 99-year leases influenced the fortunes of the farmers in different ways. In some areas, depending on the crop grown, the 99-year leases were accepted as a basis for a loan. In other instances, the leases have not been accepted as collateral and this has constrained the farmers’ ability to optimally utilise their farms optimally. Despite a general sense of insecurity, the farmers have shown a willingness to re-invest in the farms., Many improvements such as new accommodation for employees, the erection of fire guards, and the development of water sources are being made on the farms, all pointing to a desire for sustained utilisation of the A2 farms. The researcher proposes that serious effort has to be made towards making the 99-year leases acceptable as collateral for loans because otherwise, in the context of general undercapitalisation among the farmers, their ability to make the medium-scale farms optimally contribute to national accumulation will remain constrained. The form of tenure is related to the empowerment of the farmers, both materially and socially. This is in turn related to the sustainable use of the land and hence to sustainable development in Zimbabwe.
- Full Text:
- Date Issued: 2019
Effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats
- Authors: Tapiwa, Chikwanda Allen
- Date: 2016
- Subjects: Goats Goats -- Feeding and feeds Greenhouse gas mitigation , Grazing -- Environmental aspects
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2314 , vital:27752
- Description: The broad objective of this study was to evaluate the effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats. A total of 64 castrated Nguni goats were used and a housing structure was designed to accommodate the goats as well as evaluate effects of type of floor on greenhouse gases, meat quality, blood metabolites and fatty acid profiles. In the first experiment: Forty-eight castrated Nguni goats, at 6-7 months old were used for herding (H) or tethering (T) management systems and randomly allocated to pens with earth (E) or slatted (S) floors. Gas sampling was done using static chambers and analysed using a SRI 8610C gas chromatography machine. The highest CH4 fluxes were from the manure of herded goats on earth floors (HE). The highest CO2 fluxes were from manure of herded goats that were housed on slatted floors (HS): The highest N2O fluxes were from the manure of tethered goats that were sheltered on slatted floors (TS). It was concluded from the first experiment that goat manure is an important source of GHG that is influenced by grazing and floor type management systems. The second experiment was done to evaluate greenhouse gas (GHG) emission from disused (DP) vs occupied goat pens (OP) with earth (EF) or slatted (SF) floors. Eight previously occupied pens were used in this study. Sixteen goats were randomly put in the pens labelled occupied pens with earth floors (OPEF) and occupied pens with slatted floors (OPSF) resulting in four goats per OP. There were month by pen use; month by floor type; and use by floor type interaction effects (P < 0.05) on CH4 production. Floor type, use (DP vs OP) and month affected (P < 0.05) CH4 production. Manure on earth floors of occupied pens (OPEF) emitted the highest (P < 0.05) methane followed by earth floors of disused pens (DPEF). The least CH4 was emitted from DPSF. There was pen use (DP vs OP) by floor type interaction effect (P < 0.05) on CO2 emission. Floor type and pen use affected (P < 0.05) CO2 production. Manure in occupied pens with slatted floors (OPSF) produced the highest CO2 and the least CO2 was from disused pens with slatted floors (DPSF). Floor type and month influenced (P < 0.05) N2O production. It was concluded that continued use of goat pens increased GHG emissions. However, disused goat pens with manure remain an important source of GHG as they continued to emit quantifiable levels of CH4, CO2 and N2O. The third experiment was done to determine the effects of grazing and housing management systems on the concentrations of blood metabolites, activity of creatine kinase, body weight (BW), body condition score (BCS) and carcass characteristics using 48 castrated Nguni goats. Tethered goats had higher (P<0.05): urea, creatinine, total protein, globulin concentrations and more activity of CK than herded goats. On the other hand, herded goats had higher (P<0.05) plasma glucose (H: 3.39±0.020 vs T: 3.23±0.020 mmol/L), serum albumin (H: 15.30±0.147 vs T: 14.82±0.144 g/L) concentration. It was concluded that serum concentrations of all the metabolites and the activity of CK were sensitive to grazing system. Herding of goats promoted more glucose concentration and preservation of muscle protein and hence superior body weights and BCS than tethered goats. The fourth experiment was done to determine the effects of grazing and housing management systems on the activity of creatine kinase (CK) and cortisol concentration, and chevon quality. Further, the effect of muscle type [Muscularis longissimus thoracis et. Lumborum (LTL) and triceps brachii (TB)] on chevon quality was also investigated. Forty eight castrated Nguni goats aged 6- 7 months were randomly allocated to tethering (n=24) and herding (n=24) treatments. Floor type affected meat (P<0.05) pH24. Tethered goats had higher (P < 0.05) L* and CL percent than herded goats. Muscle type had a significant influence (P < 0.05) on a*, WBSF and pH. It was observed and concluded that T resulted in higher L* and more CL percent whilst SF lowered the pH24 of chevon; at the same time meat from the TB muscle was more tender, despite having a higher pH24 and more CL percent relative to LTH cuts. The fifth experiment was done to determine the effect of grazing and floor type on fatty acid composition of chevon from tethered and herded Nguni goats. Floor type had no effect on fatty acid composition. Oleic, stearic, palmitic and linoleic were the major fatty acids. Chevon from tethered goats had higher (P < 0.05) proportions of long chain polyunsaturated fatty acids (PUFAs): eicosapentaenoic, docosapentaenoic, docosahexaenoic, conjugated linoleic acid (CLA) and more total SFA, total PUFA, total Omega- 6 and total omega 3 fatty acids than herded goats. On the other hand, chevon from herded goats had more (P < 0.05) C18:1c9, C18:2t9, 12 (n-6), C20:3c11, 14, 17 (n-3) and total MUFA than tethered goats. However, there was no difference (P > 0.05) in the PUFA: SFA and n-6/n-3 fatty acid ratios between the herded and tethered goats. It was concluded that tethering promoted higher proportions of beneficial PUFAs which were more in TB than LTH muscle. The overall conclusions from the whole study were that goat manure is an important source of GHG with high CH4 being emitted from earth floors of herded goats. High CO2 and N2O are emitted from SF of herded and tethered goats, respectively. There were no major differences in meat quality between herded and tethered goats. However, herding promoted more live weight gain than tethering. On the other hand, tethering promoted more beneficial fatty acids. Therefore depending on the objective of the enterprise, smallholder farmers can safely tether or herd their goats. Further, there is need to develop technologies to harness CH4 from goat manure.
- Full Text:
- Date Issued: 2016
- Authors: Tapiwa, Chikwanda Allen
- Date: 2016
- Subjects: Goats Goats -- Feeding and feeds Greenhouse gas mitigation , Grazing -- Environmental aspects
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2314 , vital:27752
- Description: The broad objective of this study was to evaluate the effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats. A total of 64 castrated Nguni goats were used and a housing structure was designed to accommodate the goats as well as evaluate effects of type of floor on greenhouse gases, meat quality, blood metabolites and fatty acid profiles. In the first experiment: Forty-eight castrated Nguni goats, at 6-7 months old were used for herding (H) or tethering (T) management systems and randomly allocated to pens with earth (E) or slatted (S) floors. Gas sampling was done using static chambers and analysed using a SRI 8610C gas chromatography machine. The highest CH4 fluxes were from the manure of herded goats on earth floors (HE). The highest CO2 fluxes were from manure of herded goats that were housed on slatted floors (HS): The highest N2O fluxes were from the manure of tethered goats that were sheltered on slatted floors (TS). It was concluded from the first experiment that goat manure is an important source of GHG that is influenced by grazing and floor type management systems. The second experiment was done to evaluate greenhouse gas (GHG) emission from disused (DP) vs occupied goat pens (OP) with earth (EF) or slatted (SF) floors. Eight previously occupied pens were used in this study. Sixteen goats were randomly put in the pens labelled occupied pens with earth floors (OPEF) and occupied pens with slatted floors (OPSF) resulting in four goats per OP. There were month by pen use; month by floor type; and use by floor type interaction effects (P < 0.05) on CH4 production. Floor type, use (DP vs OP) and month affected (P < 0.05) CH4 production. Manure on earth floors of occupied pens (OPEF) emitted the highest (P < 0.05) methane followed by earth floors of disused pens (DPEF). The least CH4 was emitted from DPSF. There was pen use (DP vs OP) by floor type interaction effect (P < 0.05) on CO2 emission. Floor type and pen use affected (P < 0.05) CO2 production. Manure in occupied pens with slatted floors (OPSF) produced the highest CO2 and the least CO2 was from disused pens with slatted floors (DPSF). Floor type and month influenced (P < 0.05) N2O production. It was concluded that continued use of goat pens increased GHG emissions. However, disused goat pens with manure remain an important source of GHG as they continued to emit quantifiable levels of CH4, CO2 and N2O. The third experiment was done to determine the effects of grazing and housing management systems on the concentrations of blood metabolites, activity of creatine kinase, body weight (BW), body condition score (BCS) and carcass characteristics using 48 castrated Nguni goats. Tethered goats had higher (P<0.05): urea, creatinine, total protein, globulin concentrations and more activity of CK than herded goats. On the other hand, herded goats had higher (P<0.05) plasma glucose (H: 3.39±0.020 vs T: 3.23±0.020 mmol/L), serum albumin (H: 15.30±0.147 vs T: 14.82±0.144 g/L) concentration. It was concluded that serum concentrations of all the metabolites and the activity of CK were sensitive to grazing system. Herding of goats promoted more glucose concentration and preservation of muscle protein and hence superior body weights and BCS than tethered goats. The fourth experiment was done to determine the effects of grazing and housing management systems on the activity of creatine kinase (CK) and cortisol concentration, and chevon quality. Further, the effect of muscle type [Muscularis longissimus thoracis et. Lumborum (LTL) and triceps brachii (TB)] on chevon quality was also investigated. Forty eight castrated Nguni goats aged 6- 7 months were randomly allocated to tethering (n=24) and herding (n=24) treatments. Floor type affected meat (P<0.05) pH24. Tethered goats had higher (P < 0.05) L* and CL percent than herded goats. Muscle type had a significant influence (P < 0.05) on a*, WBSF and pH. It was observed and concluded that T resulted in higher L* and more CL percent whilst SF lowered the pH24 of chevon; at the same time meat from the TB muscle was more tender, despite having a higher pH24 and more CL percent relative to LTH cuts. The fifth experiment was done to determine the effect of grazing and floor type on fatty acid composition of chevon from tethered and herded Nguni goats. Floor type had no effect on fatty acid composition. Oleic, stearic, palmitic and linoleic were the major fatty acids. Chevon from tethered goats had higher (P < 0.05) proportions of long chain polyunsaturated fatty acids (PUFAs): eicosapentaenoic, docosapentaenoic, docosahexaenoic, conjugated linoleic acid (CLA) and more total SFA, total PUFA, total Omega- 6 and total omega 3 fatty acids than herded goats. On the other hand, chevon from herded goats had more (P < 0.05) C18:1c9, C18:2t9, 12 (n-6), C20:3c11, 14, 17 (n-3) and total MUFA than tethered goats. However, there was no difference (P > 0.05) in the PUFA: SFA and n-6/n-3 fatty acid ratios between the herded and tethered goats. It was concluded that tethering promoted higher proportions of beneficial PUFAs which were more in TB than LTH muscle. The overall conclusions from the whole study were that goat manure is an important source of GHG with high CH4 being emitted from earth floors of herded goats. High CO2 and N2O are emitted from SF of herded and tethered goats, respectively. There were no major differences in meat quality between herded and tethered goats. However, herding promoted more live weight gain than tethering. On the other hand, tethering promoted more beneficial fatty acids. Therefore depending on the objective of the enterprise, smallholder farmers can safely tether or herd their goats. Further, there is need to develop technologies to harness CH4 from goat manure.
- Full Text:
- Date Issued: 2016
Effects of natural antioxidants and thermal treatment on quality of meat from Bonsmara and non-descripts cattle
- Authors: Falowo, Andrew Bamidele
- Date: 2015
- Subjects: Meat -- Preservation Antioxidants Meat -- Microbiology
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2589 , vital:27906
- Description: The broad objective of this study was to determine the effects of natural antioxidants and thermal treatment on quality of meat from Bonsmara and non-descript cattle. A survey was conducted among 222 consumers in Alice (Fort Hare University community), Eastern Cape Province to determine the level of their awareness on application of antioxidants as preservatives in meat and meat products during storage. Representative samples of Muscularis longissimuss thoracis et lumborum and liver were collected from each carcass of Bonsmara (n=40) and non-descript (n=40) cattle reared on natural pasture to determine the effect of natural antioxidants and thermal treatment on their quality. The results from the survey revealed that 51.35 percent of the respondents had not heard about the use of antioxidant as preservatives in meat products. However, among the remaining respondents (48.65 percent) that were aware of antioxidant and its use as preservative, about 19 percent knew of natural antioxidants, 35 percent synthetic antioxidants while 46 percent had knowledge of both. The results further revealed that the majority of the respondents (82 percent) that had knowledge of natural antioxidants were concerned about the use of synthetic antioxidants in meat and meat products due to their health consequences. The in vitro antioxidant and antibacterial analyses of Bidens pilosa and Moringa oleifera leaf extracts revealed that they contain rich bioactive compounds. Furthermore, the addition of leaf extracts from Moringa oleifera (ML, 0.05 and 0.1 percent w/w) and Biden pilosa (BP, 0.05 and 0.1 percent w/w) to ground raw beef as a natural antioxidant were found to improve the physicochemical, oxidative stability and microbiological qualities of meat compared to the control (meat without extract) and BHT treatment (0.02 percent w/w) during 6 days storage at 40 C. Cattle breed did not have much effect on colour parameters. However, the overall pH of ground beef treated with extracts showed lower values than control and BHT treated beef. Also, ground beef samples containing extracts exhibited (P < 0.05) better colour stability, especially higher redness (a* values), than the control. The formation of TBARS in beef samples treated with extracts was significantly (P < 0.05) lower than the control and BHT treatment. The antibacterial assay of the extracts revealed an appreciable broad spectrum activity against the tested bacteria and microbial counts in ground beef samples compared to control and BHT group. Ground beef treated with plant extracts exhibited lower microbial and lactic acid bacterial counts (P < 0.05) at day 0 and 3 than control samples. Moreover, it was observed that application of sous vides thermal method did not significantly affect the fatty acids and mineral loss in beef and liver across the treatments. However, the total concentration of MUFA was lower in raw liver (20.11±2.38 - 21.08±1.23 percent) than in beef (40.22±1.90 - 42.53±1.29 percent), while total PUFA content were higher in liver (30.73±2.60 - 31.11±1.19 percent) than in beef samples (10.13±3.36 - 11.02±2.74 percent) (p < 0.05). The results also revealed that liver samples from Bonsmara and non-descript cattle had a higher percentage of intramuscular fat content of 4.67 ± 0.53 percent and 4.44 ± 0.53 percent respectively, and fat free dry matter of 27.51 ± 1.05 percent and 25.73 ± 1.05 percent, respectively, than the beef samples (p < 0.05). The concentrations of Mg (52.80±0.22 - 53.70±0.02mg/100g) and Zn (8.90±0.15 - 19.60±0.15mg/100g) were higher in beef than liver samples. The level of Ca (17.00±0.17 - 17.50 ± 0.17mg/100g) in liver was higher than that of beef samples. It was concluded that most consumers preferred the use of natural antioxidants in meat products than synthetic antioxidants due to their health risk. The effectiveness of M. oleifera and B. pilosa leaf extracts on meat quality also revealed that these plants are promising candidates as natural preservatives and their application should be considered in the meat industry. Lastly, findings from this study showed that application of the sous vide technique could protect meat products from nutritional loss during thermal treatment.
- Full Text:
- Date Issued: 2015
- Authors: Falowo, Andrew Bamidele
- Date: 2015
- Subjects: Meat -- Preservation Antioxidants Meat -- Microbiology
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2589 , vital:27906
- Description: The broad objective of this study was to determine the effects of natural antioxidants and thermal treatment on quality of meat from Bonsmara and non-descript cattle. A survey was conducted among 222 consumers in Alice (Fort Hare University community), Eastern Cape Province to determine the level of their awareness on application of antioxidants as preservatives in meat and meat products during storage. Representative samples of Muscularis longissimuss thoracis et lumborum and liver were collected from each carcass of Bonsmara (n=40) and non-descript (n=40) cattle reared on natural pasture to determine the effect of natural antioxidants and thermal treatment on their quality. The results from the survey revealed that 51.35 percent of the respondents had not heard about the use of antioxidant as preservatives in meat products. However, among the remaining respondents (48.65 percent) that were aware of antioxidant and its use as preservative, about 19 percent knew of natural antioxidants, 35 percent synthetic antioxidants while 46 percent had knowledge of both. The results further revealed that the majority of the respondents (82 percent) that had knowledge of natural antioxidants were concerned about the use of synthetic antioxidants in meat and meat products due to their health consequences. The in vitro antioxidant and antibacterial analyses of Bidens pilosa and Moringa oleifera leaf extracts revealed that they contain rich bioactive compounds. Furthermore, the addition of leaf extracts from Moringa oleifera (ML, 0.05 and 0.1 percent w/w) and Biden pilosa (BP, 0.05 and 0.1 percent w/w) to ground raw beef as a natural antioxidant were found to improve the physicochemical, oxidative stability and microbiological qualities of meat compared to the control (meat without extract) and BHT treatment (0.02 percent w/w) during 6 days storage at 40 C. Cattle breed did not have much effect on colour parameters. However, the overall pH of ground beef treated with extracts showed lower values than control and BHT treated beef. Also, ground beef samples containing extracts exhibited (P < 0.05) better colour stability, especially higher redness (a* values), than the control. The formation of TBARS in beef samples treated with extracts was significantly (P < 0.05) lower than the control and BHT treatment. The antibacterial assay of the extracts revealed an appreciable broad spectrum activity against the tested bacteria and microbial counts in ground beef samples compared to control and BHT group. Ground beef treated with plant extracts exhibited lower microbial and lactic acid bacterial counts (P < 0.05) at day 0 and 3 than control samples. Moreover, it was observed that application of sous vides thermal method did not significantly affect the fatty acids and mineral loss in beef and liver across the treatments. However, the total concentration of MUFA was lower in raw liver (20.11±2.38 - 21.08±1.23 percent) than in beef (40.22±1.90 - 42.53±1.29 percent), while total PUFA content were higher in liver (30.73±2.60 - 31.11±1.19 percent) than in beef samples (10.13±3.36 - 11.02±2.74 percent) (p < 0.05). The results also revealed that liver samples from Bonsmara and non-descript cattle had a higher percentage of intramuscular fat content of 4.67 ± 0.53 percent and 4.44 ± 0.53 percent respectively, and fat free dry matter of 27.51 ± 1.05 percent and 25.73 ± 1.05 percent, respectively, than the beef samples (p < 0.05). The concentrations of Mg (52.80±0.22 - 53.70±0.02mg/100g) and Zn (8.90±0.15 - 19.60±0.15mg/100g) were higher in beef than liver samples. The level of Ca (17.00±0.17 - 17.50 ± 0.17mg/100g) in liver was higher than that of beef samples. It was concluded that most consumers preferred the use of natural antioxidants in meat products than synthetic antioxidants due to their health risk. The effectiveness of M. oleifera and B. pilosa leaf extracts on meat quality also revealed that these plants are promising candidates as natural preservatives and their application should be considered in the meat industry. Lastly, findings from this study showed that application of the sous vide technique could protect meat products from nutritional loss during thermal treatment.
- Full Text:
- Date Issued: 2015
Effects of Vigna unguiculata and Lablab purpureus on methanogenesis, haematological parameters and the quality of meat from Xhosa Lop Ear goats
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
- Date Issued: 2017
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
- Date Issued: 2017
Electrical power output estimation model for a conical diffuser augmented wind turbine
- Authors: Masukume, Peace-Maker
- Date: 2016
- Subjects: Wind power , Wind turbines , Renewable energy sources
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1517 , vital:27404
- Description: Energy is integral to the quality of life of any society. However, meeting the demand for energy sustainably is the main challenge facing humanity. In general, non-renewable energy resources are used to supply the ever increasing energy demand. However, the extraction and processing of these resources is accompanied by the production of wastes which are a health hazard and impact negatively on climate change. Considering the finite nature of non-renewable sources, the environmental concerns which are associated with their usage and ensuring energy security, renewable energy sources have been brought in the energy supply chain. Wind energy is one of the renewable energy sources which has been supplying electrical energy to the ever increasing energy demand of humanity. Wind energy technology is a mature technology which over and above the bare (conventional) wind turbine technology has seen the development of duct augmented wind turbines. Ducts are used to encase wind turbine rotors to augment the power output of wind turbines especially in low wind speed areas. Though the technology has been under study for decades now, research indicates that there is no known model to estimate the power output of a diffuser augmented wind turbine. This thesis presents the development of the conical Diffuser Augmented Wind Turbine (DAWT) power output estimation model and its validation.
- Full Text:
- Date Issued: 2016
- Authors: Masukume, Peace-Maker
- Date: 2016
- Subjects: Wind power , Wind turbines , Renewable energy sources
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1517 , vital:27404
- Description: Energy is integral to the quality of life of any society. However, meeting the demand for energy sustainably is the main challenge facing humanity. In general, non-renewable energy resources are used to supply the ever increasing energy demand. However, the extraction and processing of these resources is accompanied by the production of wastes which are a health hazard and impact negatively on climate change. Considering the finite nature of non-renewable sources, the environmental concerns which are associated with their usage and ensuring energy security, renewable energy sources have been brought in the energy supply chain. Wind energy is one of the renewable energy sources which has been supplying electrical energy to the ever increasing energy demand of humanity. Wind energy technology is a mature technology which over and above the bare (conventional) wind turbine technology has seen the development of duct augmented wind turbines. Ducts are used to encase wind turbine rotors to augment the power output of wind turbines especially in low wind speed areas. Though the technology has been under study for decades now, research indicates that there is no known model to estimate the power output of a diffuser augmented wind turbine. This thesis presents the development of the conical Diffuser Augmented Wind Turbine (DAWT) power output estimation model and its validation.
- Full Text:
- Date Issued: 2016
Emotional intelligence training model for executive leadership in South Africa
- Authors: Els, Deon André
- Date: 2016
- Subjects: Development leadership -- South Africa , Executives -- Training of -- South Africa , Emotional intelligence -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/7398 , vital:21350
- Description: Global leadership crises and increasing executive leadership failures necessitate a new approach to executive leadership development. Globalisation results in new leadership challenges that affect people, the planet and peace across the world. Critical issues include increasing extremism and terrorism, displaced migrants fleeing to stable countries, earth warming and economic decline. The role of the individual executive leader cannot be isolated from human development challenges. Various executive leadership failures and examples of unethical leadership practises, both internationally and in South Africa, place the focus on ethical governance and emotionally matured leadership development. This study follows a nexus, based on a three-stranded cordial link between human development, emotional intelligence and executive leadership. The central themes of the United Nations Human Development’s (UNDP) reports of 1990 to 2009 as well as the Post-United Nations Human Development Report of 2015 form a framework for evaluating the relationship between human development and executive leadership. Although economic growth is central to human development, the development of people through building human capabilities and active participation to improve their lives, are the main goals of the UNDP-2015. The role of executive leadership, leadership derailment and required executive proficiencies and attributes are investigated by evaluating traditional leadership theories and approaches as a lens for investigating leadership development. The positive effect of globalisation is that it affords new approaches and opportunities for executive leadership development. Emotional intelligence-based leadership, including the role of neuro-leadership, is evaluated and an integrative approach that involves the new paradigm of leadership as a response to human development challenges and globalisation is presented. The new paradigm of integrative leadership approaches includes empirical-based authentic leadership, shared leadership and gender-based leadership. The integrative leadership models of Hatala and Passmore are selected as a framework to propose a theoretical emotional intelligence leadership model for this study. New opportunities to develop emotionally intelligent executive leaders include technology-based training, iLeadership and eLeadership in an environment without boundaries. Time constraints are identified as a key obstacle for leadership development. Various training and executive coaching strategies are evaluated and proposed to accelerate leadership development. The link between human development and executive leadership development is proposed by collective leadership approaches towards Corporate Social Responsibilities (CSR) above entrepreneurial acumen and stakeholder involvement. A positivist approach based on quantitative research using Structural Equation Modelling (SEM) is used. The primary research problem is formulated to investigate the multidimensional and complex nature of factors that influence the success of developing emotionally intelligent executive leaders in South Africa. A conceptual theoretical model comprising of factors that influence Human Development and the perceived success of Emotional Intelligence Training is developed. A total of ten independent variables that influence the two mentioned dependent variables are identified. The proposed model and envisaged hypotheses are empirically tested. The study consists of a 73-itemed questionnaire with 360 participants. The sourced data are statically analysed by means of the exploratory factor analysis (EFA) to assess the discriminate validity of the research instrument and to confirm underlying dimensions of the constructs. Cronbach-alpha coefficients are calculated for each of the identified factors by using SEM. The significance of the hypothesised relationships in the revised model is tested. The value of this study’s contribution to the body of knowledge lies within the findings, the proposed Integrative Emotional Intelligence Leadership Model and recommendations for future research. The proposed model identifies practical training approaches to accelerate executive leadership against a background of serious leadership failures in South Africa.
- Full Text:
- Date Issued: 2016
- Authors: Els, Deon André
- Date: 2016
- Subjects: Development leadership -- South Africa , Executives -- Training of -- South Africa , Emotional intelligence -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/7398 , vital:21350
- Description: Global leadership crises and increasing executive leadership failures necessitate a new approach to executive leadership development. Globalisation results in new leadership challenges that affect people, the planet and peace across the world. Critical issues include increasing extremism and terrorism, displaced migrants fleeing to stable countries, earth warming and economic decline. The role of the individual executive leader cannot be isolated from human development challenges. Various executive leadership failures and examples of unethical leadership practises, both internationally and in South Africa, place the focus on ethical governance and emotionally matured leadership development. This study follows a nexus, based on a three-stranded cordial link between human development, emotional intelligence and executive leadership. The central themes of the United Nations Human Development’s (UNDP) reports of 1990 to 2009 as well as the Post-United Nations Human Development Report of 2015 form a framework for evaluating the relationship between human development and executive leadership. Although economic growth is central to human development, the development of people through building human capabilities and active participation to improve their lives, are the main goals of the UNDP-2015. The role of executive leadership, leadership derailment and required executive proficiencies and attributes are investigated by evaluating traditional leadership theories and approaches as a lens for investigating leadership development. The positive effect of globalisation is that it affords new approaches and opportunities for executive leadership development. Emotional intelligence-based leadership, including the role of neuro-leadership, is evaluated and an integrative approach that involves the new paradigm of leadership as a response to human development challenges and globalisation is presented. The new paradigm of integrative leadership approaches includes empirical-based authentic leadership, shared leadership and gender-based leadership. The integrative leadership models of Hatala and Passmore are selected as a framework to propose a theoretical emotional intelligence leadership model for this study. New opportunities to develop emotionally intelligent executive leaders include technology-based training, iLeadership and eLeadership in an environment without boundaries. Time constraints are identified as a key obstacle for leadership development. Various training and executive coaching strategies are evaluated and proposed to accelerate leadership development. The link between human development and executive leadership development is proposed by collective leadership approaches towards Corporate Social Responsibilities (CSR) above entrepreneurial acumen and stakeholder involvement. A positivist approach based on quantitative research using Structural Equation Modelling (SEM) is used. The primary research problem is formulated to investigate the multidimensional and complex nature of factors that influence the success of developing emotionally intelligent executive leaders in South Africa. A conceptual theoretical model comprising of factors that influence Human Development and the perceived success of Emotional Intelligence Training is developed. A total of ten independent variables that influence the two mentioned dependent variables are identified. The proposed model and envisaged hypotheses are empirically tested. The study consists of a 73-itemed questionnaire with 360 participants. The sourced data are statically analysed by means of the exploratory factor analysis (EFA) to assess the discriminate validity of the research instrument and to confirm underlying dimensions of the constructs. Cronbach-alpha coefficients are calculated for each of the identified factors by using SEM. The significance of the hypothesised relationships in the revised model is tested. The value of this study’s contribution to the body of knowledge lies within the findings, the proposed Integrative Emotional Intelligence Leadership Model and recommendations for future research. The proposed model identifies practical training approaches to accelerate executive leadership against a background of serious leadership failures in South Africa.
- Full Text:
- Date Issued: 2016
Energy services and energy poverty for sustainable development in Zimbabwe
- Authors: Mupindu, Chipo
- Date: 2017
- Subjects: Sustainable development -- Zimbabwe Zimbabwe -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/8408 , vital:32743
- Description: This study examines the effects of energy poverty on sustainable development in Zimbabwe. The principal objective of this study was to analyse Zimbabwe energy poverty as a contributing factor impacting on sustainable development. Access to energy was viewed as a human right in this study and attributes of ecological modernization and energy ladder theories were incorporated in the study so as to foster sustainable development. The study developed a symbiosis between energy poverty and human development in Zimbabwe. The power shortages have caused a lot of energy poverty and load shedding in the country and this has had adverse consequences on the transformation of the economy as well as the general human capacity development in the country. The supply shortage was as a result of many factors which include the lack of investment in the power sector by government for expanded generation capacity, ageing equipment, incorrect pricing, bad debts, droughts, internal conflicts, skills flight, and government energy sector regulation, vandalisation of equipment and under supply of coal to thermal power stations. The country needs to commit itself towards improving the current situation by revising the energy policy to include alternative sources, the integration of regional power systems and construction of more power stations, and by putting research monitoring and evaluation mechanisms in place so as to improve power generation capacity which meets the demand of the country. The evidence from the findings gathered in this study led to the conclusion that the Zimbabwe energy and power sector lacks the capacity to provide adequate reliable clean energy to the nation and this has affected the development of the country.
- Full Text:
- Date Issued: 2017
- Authors: Mupindu, Chipo
- Date: 2017
- Subjects: Sustainable development -- Zimbabwe Zimbabwe -- Economic conditions
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/8408 , vital:32743
- Description: This study examines the effects of energy poverty on sustainable development in Zimbabwe. The principal objective of this study was to analyse Zimbabwe energy poverty as a contributing factor impacting on sustainable development. Access to energy was viewed as a human right in this study and attributes of ecological modernization and energy ladder theories were incorporated in the study so as to foster sustainable development. The study developed a symbiosis between energy poverty and human development in Zimbabwe. The power shortages have caused a lot of energy poverty and load shedding in the country and this has had adverse consequences on the transformation of the economy as well as the general human capacity development in the country. The supply shortage was as a result of many factors which include the lack of investment in the power sector by government for expanded generation capacity, ageing equipment, incorrect pricing, bad debts, droughts, internal conflicts, skills flight, and government energy sector regulation, vandalisation of equipment and under supply of coal to thermal power stations. The country needs to commit itself towards improving the current situation by revising the energy policy to include alternative sources, the integration of regional power systems and construction of more power stations, and by putting research monitoring and evaluation mechanisms in place so as to improve power generation capacity which meets the demand of the country. The evidence from the findings gathered in this study led to the conclusion that the Zimbabwe energy and power sector lacks the capacity to provide adequate reliable clean energy to the nation and this has affected the development of the country.
- Full Text:
- Date Issued: 2017
Enhancing parental involvement in primary schools in disadvantaged communities
- Hendricks, Charlotte Augusta
- Authors: Hendricks, Charlotte Augusta
- Date: 2014
- Subjects: Education, Primary -- Parent participation -- South Africa -- Port Elizabeth , Parent-teacher relationships -- South Africa -- Port Elizabeth , Ecological Systems Theory
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9461 , http://hdl.handle.net/10948/d1020169
- Description: The main research question guiding this study is: “What strategies can be established to enhance parental involvement in primary schools in disadvantaged communities in the Northern Areas of Port Elizabeth”? To answer this question, this study examines parents’ and teachers’ practices concerning parental involvement in the Northern Areas of Nelson Mandela Metropolitan. Parents and teachers were purposely selected from participating schools. In this qualitative study, the researcher proposed strategies to enhance parental involvement at primary schools. Qualitative research methods, associated with phenomenological inquiry were employed to explore the life-world of the participants who have had personal experience with the phenomenon of parental involvement. Data was collected through semi-structured interviews with parents and teachers. This allowed the researcher to gather in-depth information about opinions, beliefs, practices and attitudes concerning the involvement of parents in their children’s education. Bronfenbrenner’s ecosystemic theory served as the theorectical framework of this investigation. Since this study is about parental involvement in primary schools educational policies like inclusive education as well as different models of parental involvement were investigated in the literature study. The results of the study indicated several barriers to the enhancement of parental involvement for instance communication barriers between parents and teachers. The researcher established four strategies for the enhancement of parental involvement and recommended that these strategies should be employed by schools and other stakeholders. She also made further recommendations for parents, teachers, school principals, Department of Basic Education and professionals in the community.
- Full Text:
- Date Issued: 2014
- Authors: Hendricks, Charlotte Augusta
- Date: 2014
- Subjects: Education, Primary -- Parent participation -- South Africa -- Port Elizabeth , Parent-teacher relationships -- South Africa -- Port Elizabeth , Ecological Systems Theory
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:9461 , http://hdl.handle.net/10948/d1020169
- Description: The main research question guiding this study is: “What strategies can be established to enhance parental involvement in primary schools in disadvantaged communities in the Northern Areas of Port Elizabeth”? To answer this question, this study examines parents’ and teachers’ practices concerning parental involvement in the Northern Areas of Nelson Mandela Metropolitan. Parents and teachers were purposely selected from participating schools. In this qualitative study, the researcher proposed strategies to enhance parental involvement at primary schools. Qualitative research methods, associated with phenomenological inquiry were employed to explore the life-world of the participants who have had personal experience with the phenomenon of parental involvement. Data was collected through semi-structured interviews with parents and teachers. This allowed the researcher to gather in-depth information about opinions, beliefs, practices and attitudes concerning the involvement of parents in their children’s education. Bronfenbrenner’s ecosystemic theory served as the theorectical framework of this investigation. Since this study is about parental involvement in primary schools educational policies like inclusive education as well as different models of parental involvement were investigated in the literature study. The results of the study indicated several barriers to the enhancement of parental involvement for instance communication barriers between parents and teachers. The researcher established four strategies for the enhancement of parental involvement and recommended that these strategies should be employed by schools and other stakeholders. She also made further recommendations for parents, teachers, school principals, Department of Basic Education and professionals in the community.
- Full Text:
- Date Issued: 2014
Evaluating neuropsychiatric symptomology in HIV-positive patients on efavirenz in public-sector clinics and psychiatric hospitals
- Gaida, Razia, Grobler, Christoffel
- Authors: Gaida, Razia , Grobler, Christoffel
- Date: 2017
- Subjects: Drugs -- Side effects -- South Africa , HIV (Viruses) -- Enzymes , Antiretroviral agents -- South Africa , Anti-infective agents -- Side effects -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/29772 , vital:30776
- Description: Background: South Africa has the highest number of people living with human immunodeficiency virus (HIV) infection in the world. In 2014, an estimated 10.2% of the population was HIV-positive which amounted to 5.51 million people. Efavirenz forms part of the triple therapy backbone used in South Africa and is part of the firstline treatment for HIV. Efavirenz has been strongly associated with causing neuropsychiatric side effects in at least 50.0% of patients to whom it is prescribed. These side effects cause hesitation amongst healthcare professionals to prescribe this agent to patients with active mental illnesses. Aim: The aim of the study was to evaluate the neuropsychiatric side effects of efavirenz in HIV-positive psychiatric and non-psychiatric patients and to determine whether this drug may be recommended for use in an HIV-positive psychiatric patient population. Method: The study was divided into two parts, namely a quantitative portion and a qualitative portion. The quantitative study was a prospective drug utilisation study, while the qualitative portion consisted of semi-structured interviews carried out with healthcare professionals working with people living with HIV/AIDS (PLWHA). The study included five municipal clinics in the Nelson Mandela Metropole as well as two public-sector psychiatric facilities in the Eastern Cape where medical records were reviewed to obtain the information required. Patients were followed in both instances for a period of 24 weeks with follow-up assessments carried out at two, four, 12 and 24 week intervals. In terms of the qualitative study, nurses at the clinics and doctors at the hospitals were contacted and appointments for interviews were made. The interviews were recorded using a voice recorder and were transcribed and analysed using theoretical framework analysis. Results: The review of 126 medical records at the clinics revealed that no patient had suffered from or complained of a neuropsychiatric side effect. This may indicate that patients were not suffering from clinically significant side effects, side effects were not being adequately recorded by healthcare staff, or the healthcare staff were not questioning patients regarding side effects. A total of 26 hospitalised patients were followed for 24 weeks in the psychiatric facilities. Almost half of the patients using efavirenz experienced an improvement in symptoms to the extent that they were iii discharged from the facility. The majority of patients (66.7%) not on an efavirenzcontaining regimen did not improve to the point of discharge. Healthcare staff were vague when providing a definition of neuropsychiatric side effects. There were conflicting ideas on whether or not efavirenz should be used in patients with an active psychiatric illness. Conclusions: Further studies need to be performed in public-sector institutions to obtain a clearer picture of the side effects experienced by patients using efavirenz. Healthcare staff need to be encouraged to keep complete records to allow for meaningful analysis. The further integration of mental health services into existing HIV programmes is essential for holistic treatment. Patients in psychiatric hospitals demonstrated that even patients with psychiatric disorders on efavirenz can experience positive outcomes and stabilisation of psychiatric symptoms, which may indicate that these may not have due to efavirenz use. Further elucidation concerning the use of efavirenz in patients with psychiatric disorders, a description of the neuropsychiatric side effects, as well as management strategies must be provided in subsequent HIV guidelines.
- Full Text:
- Date Issued: 2017
- Authors: Gaida, Razia , Grobler, Christoffel
- Date: 2017
- Subjects: Drugs -- Side effects -- South Africa , HIV (Viruses) -- Enzymes , Antiretroviral agents -- South Africa , Anti-infective agents -- Side effects -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/29772 , vital:30776
- Description: Background: South Africa has the highest number of people living with human immunodeficiency virus (HIV) infection in the world. In 2014, an estimated 10.2% of the population was HIV-positive which amounted to 5.51 million people. Efavirenz forms part of the triple therapy backbone used in South Africa and is part of the firstline treatment for HIV. Efavirenz has been strongly associated with causing neuropsychiatric side effects in at least 50.0% of patients to whom it is prescribed. These side effects cause hesitation amongst healthcare professionals to prescribe this agent to patients with active mental illnesses. Aim: The aim of the study was to evaluate the neuropsychiatric side effects of efavirenz in HIV-positive psychiatric and non-psychiatric patients and to determine whether this drug may be recommended for use in an HIV-positive psychiatric patient population. Method: The study was divided into two parts, namely a quantitative portion and a qualitative portion. The quantitative study was a prospective drug utilisation study, while the qualitative portion consisted of semi-structured interviews carried out with healthcare professionals working with people living with HIV/AIDS (PLWHA). The study included five municipal clinics in the Nelson Mandela Metropole as well as two public-sector psychiatric facilities in the Eastern Cape where medical records were reviewed to obtain the information required. Patients were followed in both instances for a period of 24 weeks with follow-up assessments carried out at two, four, 12 and 24 week intervals. In terms of the qualitative study, nurses at the clinics and doctors at the hospitals were contacted and appointments for interviews were made. The interviews were recorded using a voice recorder and were transcribed and analysed using theoretical framework analysis. Results: The review of 126 medical records at the clinics revealed that no patient had suffered from or complained of a neuropsychiatric side effect. This may indicate that patients were not suffering from clinically significant side effects, side effects were not being adequately recorded by healthcare staff, or the healthcare staff were not questioning patients regarding side effects. A total of 26 hospitalised patients were followed for 24 weeks in the psychiatric facilities. Almost half of the patients using efavirenz experienced an improvement in symptoms to the extent that they were iii discharged from the facility. The majority of patients (66.7%) not on an efavirenzcontaining regimen did not improve to the point of discharge. Healthcare staff were vague when providing a definition of neuropsychiatric side effects. There were conflicting ideas on whether or not efavirenz should be used in patients with an active psychiatric illness. Conclusions: Further studies need to be performed in public-sector institutions to obtain a clearer picture of the side effects experienced by patients using efavirenz. Healthcare staff need to be encouraged to keep complete records to allow for meaningful analysis. The further integration of mental health services into existing HIV programmes is essential for holistic treatment. Patients in psychiatric hospitals demonstrated that even patients with psychiatric disorders on efavirenz can experience positive outcomes and stabilisation of psychiatric symptoms, which may indicate that these may not have due to efavirenz use. Further elucidation concerning the use of efavirenz in patients with psychiatric disorders, a description of the neuropsychiatric side effects, as well as management strategies must be provided in subsequent HIV guidelines.
- Full Text:
- Date Issued: 2017
Evaluating the effectiveness of public participation in the environmental impact assessment process in South Africa
- Authors: Uithaler, Eldrid Marlon
- Date: 2015
- Subjects: Environmental impact analysis -- South Africa , Environmental policy -- Citizen participation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/5928 , vital:21015
- Description: This research is based on reflections of various practitioners and their views on how public participation processes may or may not contribute to effective EIA decisions. It is therefore grounded in what is described as practitioner-based research. To study comparative development activities effectively, one must draw on many disciplines and construct a balanced understanding of historical and contemporary development processes. No single conventional disciplinary area of research is able to integrate the issues of public participation, EIA and decision-making in the context of the current development debate. The emergence of Trans-disciplinary areas of research allows for such integration. The Africa Earth Observatory Network (AEON) institute creates the space for this study to achieve an integrated response to the question of the effectiveness of public participation in EIA and decision-making. This further creates the opportunity to contribute and expand the growing body of knowledge and literature of public participation in the earth stewardship science discipline. Making use of triangulation, this study fulfils four major tasks: firstly, an assessment is made on the historical and theoretical importance, process and outcome of both EIA and public participation internationally and in South Africa. Secondly, three prominent case studies (i) the Coega IDZ, (ii) the Pondoland N2 Toll Road, and (iii) the anticipated Hydraulic Fracturing in the Karoo each focusses on concerns of an environmental, socio-economic, and political nature to assess if the public participation process has had influence, if any, on the final decisions for these projects to go-ahead. Thirdly, a survey was conducted to establish the views and perceptions of practitioners in the EIA and public participation domain on the effectiveness of public participation in EIA processes. Lastly, face-to-face interviews were conducted with various ‘pracademics’, i.e. consultants, practitioners, government, and non-governmental officials to establish their views on how public participation may or may not influence EIA decisions. Utilising the International Association for Public Participation’s (IAP2) participation spectrum as an evaluation tool, this research explores thirteen key criteria normally attributed to effective public participation. This provides a scale (inform, involve, consult, collaborate) to assess whether public participation in EIA in South Africa is least effective (inform) or most effective (collaborate). My research confirms that in South Africa an enabling environment to address impacts on our environment is emerging. One of the main challenges remains putting in place robust, clear and effective regulations, models or approaches that provide for effective public participation and decision-making in EIAs. My research also suggests that the legislation on which EIA is based cannot by itself guarantee the effectiveness of public participation processes. In practice, EIA is an institutional process of power division between different actors, and the practitioner or ‘pracademic’ has to play a more fundamental role to ensure effectiveness and fairness in the public participation process. Academic commentators should therefore call for new approaches that emphasise collaborative interaction between decision-makers and the public as well as deliberation amongst participants.
- Full Text:
- Date Issued: 2015
- Authors: Uithaler, Eldrid Marlon
- Date: 2015
- Subjects: Environmental impact analysis -- South Africa , Environmental policy -- Citizen participation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/5928 , vital:21015
- Description: This research is based on reflections of various practitioners and their views on how public participation processes may or may not contribute to effective EIA decisions. It is therefore grounded in what is described as practitioner-based research. To study comparative development activities effectively, one must draw on many disciplines and construct a balanced understanding of historical and contemporary development processes. No single conventional disciplinary area of research is able to integrate the issues of public participation, EIA and decision-making in the context of the current development debate. The emergence of Trans-disciplinary areas of research allows for such integration. The Africa Earth Observatory Network (AEON) institute creates the space for this study to achieve an integrated response to the question of the effectiveness of public participation in EIA and decision-making. This further creates the opportunity to contribute and expand the growing body of knowledge and literature of public participation in the earth stewardship science discipline. Making use of triangulation, this study fulfils four major tasks: firstly, an assessment is made on the historical and theoretical importance, process and outcome of both EIA and public participation internationally and in South Africa. Secondly, three prominent case studies (i) the Coega IDZ, (ii) the Pondoland N2 Toll Road, and (iii) the anticipated Hydraulic Fracturing in the Karoo each focusses on concerns of an environmental, socio-economic, and political nature to assess if the public participation process has had influence, if any, on the final decisions for these projects to go-ahead. Thirdly, a survey was conducted to establish the views and perceptions of practitioners in the EIA and public participation domain on the effectiveness of public participation in EIA processes. Lastly, face-to-face interviews were conducted with various ‘pracademics’, i.e. consultants, practitioners, government, and non-governmental officials to establish their views on how public participation may or may not influence EIA decisions. Utilising the International Association for Public Participation’s (IAP2) participation spectrum as an evaluation tool, this research explores thirteen key criteria normally attributed to effective public participation. This provides a scale (inform, involve, consult, collaborate) to assess whether public participation in EIA in South Africa is least effective (inform) or most effective (collaborate). My research confirms that in South Africa an enabling environment to address impacts on our environment is emerging. One of the main challenges remains putting in place robust, clear and effective regulations, models or approaches that provide for effective public participation and decision-making in EIAs. My research also suggests that the legislation on which EIA is based cannot by itself guarantee the effectiveness of public participation processes. In practice, EIA is an institutional process of power division between different actors, and the practitioner or ‘pracademic’ has to play a more fundamental role to ensure effectiveness and fairness in the public participation process. Academic commentators should therefore call for new approaches that emphasise collaborative interaction between decision-makers and the public as well as deliberation amongst participants.
- Full Text:
- Date Issued: 2015
Evaluating the impact of energy policies on sustainable development in South Africa
- Authors: Julius, Ibitoye Oyebanji
- Date: 2018
- Subjects: Energy policy -- South Africa , Sustainable development -- South Africa Renewable energy sources -- South Africa Sustainable living -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30440 , vital:30944
- Description: The unachieved targets of the Millennium Development Goals (MDGs) at the end of 2015 by some developing countries including South Africa, lead to the evolution of the Sustainable Development Goals (SDGs). Ensuring access to sustainable, affordable, and modern energy is one of the 17 SDGs that the countries are expected to achieve before the end of 2030. Green growth is an important strategy for attaining this goal and a pathway to achieving the other goals. Therefore, an empirical study of the impact of energy policies on sustainable development is pertinent. Despite the importance of such research, there exists a gap in the literature relating to green growth and sustainable development from the SDGs point of view. This study fills the existing lacuna by developing an intertemporal holistic model which allows a study of the impact of energy policies on sustainable development in relation to the SDGs. Specifically, this thesis employs the Autoregressive Distributed Lag (ARDL) and Toda-Yamamoto approaches to analyse the long-run relationship and direction of causality respectively between green growth policies and sustainable development in South Africa from 1984 to 2016. This research provides insights into the importance of green growth for factors such as deforestation, fuel exports, energy imports and agricultural development, thus adding to the existing literature on the impact of renewable energy on a country’s social, environmental and economic conditions. Results from the analysis of the long-run relationship between green growth and macroeconomic variables, indicate a positive unidirectional relationship between changes in green growth policies and gross capital formation. This finding suggests that adopting green growth policies leads to increased investments. In contrast, green growth was found to have a negative effect on national income (gross domestic product). However, the Granger causality tests do not establish causality between these variables. Although green growth policies stimulate investments, high start-up costs associated with the implementation of these policies may mean that the resultant higher investments are yet to be translated to higher income levels in the South African economy. The findings indicate a boost in the South African balance of payment within the study period iv iv as evidenced by the positive long-run relationship between green growth policies and fuel exports. Additionally, the results indicate that green growth energy policies have assisted in improving the resilience to international oil price shocks as shown in the negative and significant long-run relationship between green growth and energy imports. With regard to environmental variables, results suggest a substitutability effect between green growth policies and the consumption of non-renewable energy. However, no evidence is found on the impact of green growth policies on the levels of deforestation and carbon dioxide(CO2) emissions, as well as natural resource depletion. In relation to the social variables, adoption of green growth policies was found to lead to improved educational quality, with causality running in both directions. This finding supports the feedback hypothesis. Similarly, green growth policies had a positive impact on agricultural development, again with causality established in both directions. The outcome of the long-run relationship between green energy consumption and unemployment shows that the green growth policies is yet to have a significant impact on unemployment reduction in South Africa. Green growth also has an insignificant and indirect relationship with GDP per capita; this result was affirmed by the absence of causality between GDP per capita and green energy consumption. This research not only makes contributions to the literature on the relevance of green growth policies for achieving economic, social and environmental sustainability, it also suggests possible policy implications which highlight the need for adoption and continued implementation of green growth policies in developing countries such as South Africa.
- Full Text:
- Date Issued: 2018
- Authors: Julius, Ibitoye Oyebanji
- Date: 2018
- Subjects: Energy policy -- South Africa , Sustainable development -- South Africa Renewable energy sources -- South Africa Sustainable living -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/30440 , vital:30944
- Description: The unachieved targets of the Millennium Development Goals (MDGs) at the end of 2015 by some developing countries including South Africa, lead to the evolution of the Sustainable Development Goals (SDGs). Ensuring access to sustainable, affordable, and modern energy is one of the 17 SDGs that the countries are expected to achieve before the end of 2030. Green growth is an important strategy for attaining this goal and a pathway to achieving the other goals. Therefore, an empirical study of the impact of energy policies on sustainable development is pertinent. Despite the importance of such research, there exists a gap in the literature relating to green growth and sustainable development from the SDGs point of view. This study fills the existing lacuna by developing an intertemporal holistic model which allows a study of the impact of energy policies on sustainable development in relation to the SDGs. Specifically, this thesis employs the Autoregressive Distributed Lag (ARDL) and Toda-Yamamoto approaches to analyse the long-run relationship and direction of causality respectively between green growth policies and sustainable development in South Africa from 1984 to 2016. This research provides insights into the importance of green growth for factors such as deforestation, fuel exports, energy imports and agricultural development, thus adding to the existing literature on the impact of renewable energy on a country’s social, environmental and economic conditions. Results from the analysis of the long-run relationship between green growth and macroeconomic variables, indicate a positive unidirectional relationship between changes in green growth policies and gross capital formation. This finding suggests that adopting green growth policies leads to increased investments. In contrast, green growth was found to have a negative effect on national income (gross domestic product). However, the Granger causality tests do not establish causality between these variables. Although green growth policies stimulate investments, high start-up costs associated with the implementation of these policies may mean that the resultant higher investments are yet to be translated to higher income levels in the South African economy. The findings indicate a boost in the South African balance of payment within the study period iv iv as evidenced by the positive long-run relationship between green growth policies and fuel exports. Additionally, the results indicate that green growth energy policies have assisted in improving the resilience to international oil price shocks as shown in the negative and significant long-run relationship between green growth and energy imports. With regard to environmental variables, results suggest a substitutability effect between green growth policies and the consumption of non-renewable energy. However, no evidence is found on the impact of green growth policies on the levels of deforestation and carbon dioxide(CO2) emissions, as well as natural resource depletion. In relation to the social variables, adoption of green growth policies was found to lead to improved educational quality, with causality running in both directions. This finding supports the feedback hypothesis. Similarly, green growth policies had a positive impact on agricultural development, again with causality established in both directions. The outcome of the long-run relationship between green energy consumption and unemployment shows that the green growth policies is yet to have a significant impact on unemployment reduction in South Africa. Green growth also has an insignificant and indirect relationship with GDP per capita; this result was affirmed by the absence of causality between GDP per capita and green energy consumption. This research not only makes contributions to the literature on the relevance of green growth policies for achieving economic, social and environmental sustainability, it also suggests possible policy implications which highlight the need for adoption and continued implementation of green growth policies in developing countries such as South Africa.
- Full Text:
- Date Issued: 2018
Evaluation of flocculating potentials and charecterization of bioflocculants produced by three bacterial isolates from Algoa Bay, South Africa
- Authors: Okaiyeto, Kunle
- Date: 2016
- Subjects: Flocculation Water -- Purification -- Flocculation Water quality management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2633 , vital:27947
- Description: Flocculation has been widely adopted as one of the most effective methods to remove colloidal particles in water or wastewater treatment. Synthetic flocculants are conventionally used because of their high flocculating efficiency and cost-effectiveness. However, they have been reported to have hazardous properties and implicated in some serious health problems including senile dementia and neuro-toxicity, as well as being recalcitrant in the environment. Consequently, efforts are being geared away from the use of synthetic flocculants in water and wastewater treatment. Hence, the need for safe and eco-friendly flocculants has become imperative. Compared with synthetic flocculants, bioflocculants have special advantages such as safety, biodegradability and harmlessness to the environment and humans; attributes which make them potential alternatives in water treatment, downstream as well as fermentation processes. In the current study, the potentials of bacterial isolates recovered from Algoa Bay in the Eastern Cape Province of South Africa for bioflocculant production were investigated. The bacterial isolates were identified by polymerase chain reaction (PCR) as belonging to the Bacillus genus. The analysis of 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of isolate M72 showed 99 percent similarity to Bacillus toyonensis strain BCT-7112 and was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. Likewise, the 16S rDNA nucleotide sequences of isolates M69 and M67 showed 98 percent sequence similarity to Bacillus licheniformis strain W7 and Bacillus algicola strain QD43 respectively; and M67 isolate was subsequently deposited in the GenBank as Bacillus sp. AEMREG7 with accession number KF933697.1. The results of the nutritional requirements and fermentation conditions revealed that optimum inoculum size for REG-6 production was 4 percent (v/v), while 5 percent (v/v) and 3 percent (v/v) were most favourable for MBF-W7 and MBF-UFH production respectively. Glucose was the best carbon source for the production of bioflocculants (REG-6 and MBF-UFH) by Bacillus toyonensis AEMREG6 and Bacillus sp. AEMREG7 respectively, while maltose supported optimum bioflocculant (MBF-W7) production by Bacillus specie. Inorganic nitrogen (NH4NO3) was the favoured nitrogen source for both REG-6 and MBF-W7 production, while mixed nitrogen sources [yeast extract + urea + (NH4)2SO4] supported the maximum production of MBF-UFH. The initial medium pH for REG-6 was 5, while MBF-W7 and MBF-UFH were both maximally produced at the initial pH of 6. After a 96 h cultivation period under optimal culture conditions, 3.2 g of purified REG-6 with a maximum flocculating activity of 77 percent was recovered from 1 L fermented broth of Bacillus toyonensis AEMREG6. Yields of 3.8 g and 1.6 g pure bioflocculants with the respective highest flocculating activities of 94.9 percent and 83.2 percent were also obtained from 1 L, 72 h-fermented broths of Bacillus licheniformis and Bacillus sp. AEMREG7 respectively. Furthermore, all the three bioflocculants (REG-6, MBF-W7 and MBF-UFH), displayed thermal stability within the temperature range of 50 to 100 oC, with strong flocculating activities of over 80 percent against kaolin suspension over a wide range of pH range (3–11) and relatively low dosage requirements of 0.1-03 mg/ml in the presence of divalent cations in the treatment of kaolin clay suspension and Thyme River waters. Chemical composition analyses of the bioflocculants showed them to be glycoproteins with a predominantly polysaccharide backbones as shown by the following carbohydrate/protein (w/w) ratios: 77.8 percent:11.5 percent (REG-6); 73.7 percent:6.2 percent (MBF-W7) and 76 percent:14 percent (MBF-UFH).
- Full Text:
- Date Issued: 2016
- Authors: Okaiyeto, Kunle
- Date: 2016
- Subjects: Flocculation Water -- Purification -- Flocculation Water quality management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2633 , vital:27947
- Description: Flocculation has been widely adopted as one of the most effective methods to remove colloidal particles in water or wastewater treatment. Synthetic flocculants are conventionally used because of their high flocculating efficiency and cost-effectiveness. However, they have been reported to have hazardous properties and implicated in some serious health problems including senile dementia and neuro-toxicity, as well as being recalcitrant in the environment. Consequently, efforts are being geared away from the use of synthetic flocculants in water and wastewater treatment. Hence, the need for safe and eco-friendly flocculants has become imperative. Compared with synthetic flocculants, bioflocculants have special advantages such as safety, biodegradability and harmlessness to the environment and humans; attributes which make them potential alternatives in water treatment, downstream as well as fermentation processes. In the current study, the potentials of bacterial isolates recovered from Algoa Bay in the Eastern Cape Province of South Africa for bioflocculant production were investigated. The bacterial isolates were identified by polymerase chain reaction (PCR) as belonging to the Bacillus genus. The analysis of 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of isolate M72 showed 99 percent similarity to Bacillus toyonensis strain BCT-7112 and was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. Likewise, the 16S rDNA nucleotide sequences of isolates M69 and M67 showed 98 percent sequence similarity to Bacillus licheniformis strain W7 and Bacillus algicola strain QD43 respectively; and M67 isolate was subsequently deposited in the GenBank as Bacillus sp. AEMREG7 with accession number KF933697.1. The results of the nutritional requirements and fermentation conditions revealed that optimum inoculum size for REG-6 production was 4 percent (v/v), while 5 percent (v/v) and 3 percent (v/v) were most favourable for MBF-W7 and MBF-UFH production respectively. Glucose was the best carbon source for the production of bioflocculants (REG-6 and MBF-UFH) by Bacillus toyonensis AEMREG6 and Bacillus sp. AEMREG7 respectively, while maltose supported optimum bioflocculant (MBF-W7) production by Bacillus specie. Inorganic nitrogen (NH4NO3) was the favoured nitrogen source for both REG-6 and MBF-W7 production, while mixed nitrogen sources [yeast extract + urea + (NH4)2SO4] supported the maximum production of MBF-UFH. The initial medium pH for REG-6 was 5, while MBF-W7 and MBF-UFH were both maximally produced at the initial pH of 6. After a 96 h cultivation period under optimal culture conditions, 3.2 g of purified REG-6 with a maximum flocculating activity of 77 percent was recovered from 1 L fermented broth of Bacillus toyonensis AEMREG6. Yields of 3.8 g and 1.6 g pure bioflocculants with the respective highest flocculating activities of 94.9 percent and 83.2 percent were also obtained from 1 L, 72 h-fermented broths of Bacillus licheniformis and Bacillus sp. AEMREG7 respectively. Furthermore, all the three bioflocculants (REG-6, MBF-W7 and MBF-UFH), displayed thermal stability within the temperature range of 50 to 100 oC, with strong flocculating activities of over 80 percent against kaolin suspension over a wide range of pH range (3–11) and relatively low dosage requirements of 0.1-03 mg/ml in the presence of divalent cations in the treatment of kaolin clay suspension and Thyme River waters. Chemical composition analyses of the bioflocculants showed them to be glycoproteins with a predominantly polysaccharide backbones as shown by the following carbohydrate/protein (w/w) ratios: 77.8 percent:11.5 percent (REG-6); 73.7 percent:6.2 percent (MBF-W7) and 76 percent:14 percent (MBF-UFH).
- Full Text:
- Date Issued: 2016
Evaluation of the incidence of enteric viruses, Vibrio species and Escherichia coli pathotypes in effluents of two wastewater treatment plants located in Keiskammahoek and Stutterheim in the Eastern Cape Province of South Africa
- Authors: Adefisoye, Martins Ajibade
- Date: 2016
- Subjects: Escherichia coli -- South Africa -- Eastern Cape Water -- Purification -- South Africa -- Eastern Cape Effluent quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/3165 , vital:28328
- Description: South Africa is currently experiencing water shortage crisis, a challenge that has been attributed not only to the scarcity of freshwater, but also to fast degrading water quality. Factors such as rapid urbanisation, population and economic growth, climate change as well as poor operational and maintenance of many of the exisiting water/wastewater treatment facilities have been acknowledged as important contributors to degrading water quality in the country. Untreated or inadequately treated discharged wastewater effluents constitute point source pollution to many freshwater environments in South Africa. Hence, it becomes imperative to evaluate wastewater discharges in other to protect the scarce freshwater resource, the environment and public health. Over a twelve-month sampling period (September 2012 to August 2013), we assessed the bacteriological, virological and physicochemical qualities of the discharged final effluents of two wastewater treatment facilities in the Eastern Cape Province of South Africa. For the physicochemical assessment, a total of 144 final effluent samples were collected from both the final effluent tanks (FE) and the discharge points (DP) of the treatment facilities. Physicochemical parameters including pH, temperature, turbidity, total dissolved solids (TDS), dissolved oxygen (DO), electrical conductivity (EC) and free chlorine concentration were determined on site while biological oxygen demand (BOD), nitrate (NO3-), nitrite (NO2-), phosphate (PO4-) and chemical oxygen demand (COD) were determined in the laboratory. The bacteriological analysis of the samples was done using standard membrane filtration (MF) technique. Bacterial group assessed included: faecal indicator bacteria (faecal coliforms and E. coli) and Vibrio species, while the antibiotic susceptibility profiles of selected E. coli and Vibrio species isolates against some selected antibiotics commonly used in human therapy and veterinary medicine were determind using the standard agar-disc diffusion method. The occurrence and concentrations of human enteric viruses including: human adenovirus (HAdV), hepatitis A virus (HAV) and rotavirus (RoV) in the samples were determined by TaqMan-based real-time polymerase chain reaction (qPCR) following concentration by adsorption-elution method. The physicochemical characteristics of the samples ranged as follows: pH (6.5 – 7.6), TDS (95 – 171 mg/L), EC (134 – 267 μS/cm), temperature (12 – 27 °C), turbidity (1.5 – 65.7 mg/L), free chlorine (0.08 – 0.72 mg/L), DO (2.06 – 9.81 mg/L), BOD (0.13 – 9.81 mg/L), NO3- (0 – 21.5 mg/L), NO2- (0 – 0.72 mg/L), PO4- (0 – 18.3 mg/L) and COD (27 – 680 mg/L). Some of the characteristic such as pH, TDS, EC, temperature, nitrite and DO (on most instances) complied with recommended guidelines. Other characteristics, however, including turbidity, BOD, nitrate, phosphate and COD fell short of the recommended guidelines. All the 48 samples analysed for bacteriological qualities tested positive for the presence of the bacterial groups with significant (P≤0.05) seasonal variation in their densities. Faecal coliforms were detected in counts ranging from 1 CFU/100ml to 2.7 × 104 CFU/100ml. Presumptive E. coli counts ranged generally between 1 CFU/100ml – 1.4 × 105 CFU/100ml while counts of presumptive Vibrio species ranged between 4 CFU/100ml – 1.4 × 104 CFU/100ml. Molecular identification of the presumptive isolates by polymerase chain reactions PCR gave positive reaction rates of 76.2 percent (381/500) and 69.8 percent (279/400) for E.coli and Vibrio species respectively. The antibiotic susceptibility profiling of 205 PCR-confirmed Vibiro isolates against 18 commomly used antibiotics showed resistance frequencies ranging from 0.5 percent (imipenem) to 96.1 percent (penicillin G) at recommended breakpoint concentrations. Eighty-one percent (166/205) of the Vibrio isolates showed multidrug resistance (resistance to 3 or more antibiotics) with the most common multiple antibiotic resistance phenotype (MARP) being AP-T-TM-SMX-PG-NI-PB, occurring in 8 isolates.
- Full Text:
- Date Issued: 2016
- Authors: Adefisoye, Martins Ajibade
- Date: 2016
- Subjects: Escherichia coli -- South Africa -- Eastern Cape Water -- Purification -- South Africa -- Eastern Cape Effluent quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/3165 , vital:28328
- Description: South Africa is currently experiencing water shortage crisis, a challenge that has been attributed not only to the scarcity of freshwater, but also to fast degrading water quality. Factors such as rapid urbanisation, population and economic growth, climate change as well as poor operational and maintenance of many of the exisiting water/wastewater treatment facilities have been acknowledged as important contributors to degrading water quality in the country. Untreated or inadequately treated discharged wastewater effluents constitute point source pollution to many freshwater environments in South Africa. Hence, it becomes imperative to evaluate wastewater discharges in other to protect the scarce freshwater resource, the environment and public health. Over a twelve-month sampling period (September 2012 to August 2013), we assessed the bacteriological, virological and physicochemical qualities of the discharged final effluents of two wastewater treatment facilities in the Eastern Cape Province of South Africa. For the physicochemical assessment, a total of 144 final effluent samples were collected from both the final effluent tanks (FE) and the discharge points (DP) of the treatment facilities. Physicochemical parameters including pH, temperature, turbidity, total dissolved solids (TDS), dissolved oxygen (DO), electrical conductivity (EC) and free chlorine concentration were determined on site while biological oxygen demand (BOD), nitrate (NO3-), nitrite (NO2-), phosphate (PO4-) and chemical oxygen demand (COD) were determined in the laboratory. The bacteriological analysis of the samples was done using standard membrane filtration (MF) technique. Bacterial group assessed included: faecal indicator bacteria (faecal coliforms and E. coli) and Vibrio species, while the antibiotic susceptibility profiles of selected E. coli and Vibrio species isolates against some selected antibiotics commonly used in human therapy and veterinary medicine were determind using the standard agar-disc diffusion method. The occurrence and concentrations of human enteric viruses including: human adenovirus (HAdV), hepatitis A virus (HAV) and rotavirus (RoV) in the samples were determined by TaqMan-based real-time polymerase chain reaction (qPCR) following concentration by adsorption-elution method. The physicochemical characteristics of the samples ranged as follows: pH (6.5 – 7.6), TDS (95 – 171 mg/L), EC (134 – 267 μS/cm), temperature (12 – 27 °C), turbidity (1.5 – 65.7 mg/L), free chlorine (0.08 – 0.72 mg/L), DO (2.06 – 9.81 mg/L), BOD (0.13 – 9.81 mg/L), NO3- (0 – 21.5 mg/L), NO2- (0 – 0.72 mg/L), PO4- (0 – 18.3 mg/L) and COD (27 – 680 mg/L). Some of the characteristic such as pH, TDS, EC, temperature, nitrite and DO (on most instances) complied with recommended guidelines. Other characteristics, however, including turbidity, BOD, nitrate, phosphate and COD fell short of the recommended guidelines. All the 48 samples analysed for bacteriological qualities tested positive for the presence of the bacterial groups with significant (P≤0.05) seasonal variation in their densities. Faecal coliforms were detected in counts ranging from 1 CFU/100ml to 2.7 × 104 CFU/100ml. Presumptive E. coli counts ranged generally between 1 CFU/100ml – 1.4 × 105 CFU/100ml while counts of presumptive Vibrio species ranged between 4 CFU/100ml – 1.4 × 104 CFU/100ml. Molecular identification of the presumptive isolates by polymerase chain reactions PCR gave positive reaction rates of 76.2 percent (381/500) and 69.8 percent (279/400) for E.coli and Vibrio species respectively. The antibiotic susceptibility profiling of 205 PCR-confirmed Vibiro isolates against 18 commomly used antibiotics showed resistance frequencies ranging from 0.5 percent (imipenem) to 96.1 percent (penicillin G) at recommended breakpoint concentrations. Eighty-one percent (166/205) of the Vibrio isolates showed multidrug resistance (resistance to 3 or more antibiotics) with the most common multiple antibiotic resistance phenotype (MARP) being AP-T-TM-SMX-PG-NI-PB, occurring in 8 isolates.
- Full Text:
- Date Issued: 2016
Evidence-informed clinical guidelines for nursing care practices related to the safety of the mechanically ventilated patient
- Authors: Jordan, Portia Janine
- Date: 2011
- Subjects: Respiratory intensive care -- South Africa -- Port Elizabeth , Respiratory therapy -- South Africa -- Port Elizabeth , Artificial respiration -- South Africa -- Port Elizabeth , Artificial respiration -- Nursing , Respirators (Medical equipment) -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10027 , http://hdl.handle.net/10948/1322 , Respiratory intensive care -- South Africa -- Port Elizabeth , Respiratory therapy -- South Africa -- Port Elizabeth , Artificial respiration -- South Africa -- Port Elizabeth , Artificial respiration -- Nursing , Respirators (Medical equipment) -- South Africa -- Port Elizabeth
- Description: An evidence-based approach to clinical practice aims to deliver appropriate care in an efficient manner to individual patients. This approach entails the integration of research evidence, clinical expertise and the interpretation of patients' needs and perspectives in making decisions about the best care modalities. The increased emphasis internationally on improved patient care and cost effectiveness in health care delivery highlighted the need for quality health services that have to be built upon the use of best evidence to inform practice and patient-care decision-making (McKenna, Ashton and Keeney, 2004:178). Critical care nursing science, a specialised branch of nursing, focusing on the care of the critically ill patient in a designated unit, is no exception to the drive to provide improved quality and cost-effective patient care. Critical care practitioners are seen to have a specialised knowledge base, specific skills in delivering advanced health care and a commitment to serve the critically ill patient. It is expected of them to be aware of new and emerging evidence about health disease processes, treatment modalities and technology used in the critical-care units. Due to the dynamic nature of a critical care unit, it is essential that every practitioner working in the unit, whether a novice professional nurse or senior unit manager, needs to be aware of the current evidence guiding their practices (Elliot, Aitken, Chaboyer, 2007:18). With reference to the critically ill patient who is connected to a mechanical ventilator, practices related to the nursing care of this group of patients, who mostly occupy the critical care units, should be based on the best evidence in order to provide cost-effective and quality care. The research study aimed to explore and describe four identified nursing care practices related to safety of a mechanically ventilated patient as performed by professional nurses in the critical care units in the Nelson Mandela Metropole. The identified nursing-care practices include: endotrachael tube placement verification, endotracheal tube cuff pressure monitoring, endotrachael tube suctioning and mechanical ventilator settings. This objective was operationalized in Stage One of the study, by using a quantitative, explorative, descriptive and contextual approach. A structured questionnaire was utilised to collect data from professional nurses working in critical care units. From the analysed data, it was decided to select the two nursing care practices that were done least according to the best recommended practice, namely endotracheal tube suctioning and endotracheal tube cuff pressure monitoring. Based on the results, systematic reviews were done respectively on the two nursing care practices. On completion of Stage One of the study, evidence-informed clinical guidelines for the two identified nursing care practices were developed. The clinical guidelines were based on the evidence found in conducting the systematic reviews. The draft clinical guidelines were reviewed by an expert panel. Feedback from the reviewers was considered to prepare the final evidence-informed clinical guidelines. Based on the clinical guidelines, two clinical algorithms were developed, which might be used at the patient's bedside and can assist in quick dissemination of the recommendations for practice. Ethical considerations were maintained throughout the study. The quality of the study was ensured in applying the principles of validity and reliability as well as performing a critical appraisal of all data collected during the systematic review. It is envisaged that the study findings be disseminated in the critical care units in the Nelson Mandela Metropole and published in peer reviewed journals.
- Full Text:
- Date Issued: 2011
- Authors: Jordan, Portia Janine
- Date: 2011
- Subjects: Respiratory intensive care -- South Africa -- Port Elizabeth , Respiratory therapy -- South Africa -- Port Elizabeth , Artificial respiration -- South Africa -- Port Elizabeth , Artificial respiration -- Nursing , Respirators (Medical equipment) -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10027 , http://hdl.handle.net/10948/1322 , Respiratory intensive care -- South Africa -- Port Elizabeth , Respiratory therapy -- South Africa -- Port Elizabeth , Artificial respiration -- South Africa -- Port Elizabeth , Artificial respiration -- Nursing , Respirators (Medical equipment) -- South Africa -- Port Elizabeth
- Description: An evidence-based approach to clinical practice aims to deliver appropriate care in an efficient manner to individual patients. This approach entails the integration of research evidence, clinical expertise and the interpretation of patients' needs and perspectives in making decisions about the best care modalities. The increased emphasis internationally on improved patient care and cost effectiveness in health care delivery highlighted the need for quality health services that have to be built upon the use of best evidence to inform practice and patient-care decision-making (McKenna, Ashton and Keeney, 2004:178). Critical care nursing science, a specialised branch of nursing, focusing on the care of the critically ill patient in a designated unit, is no exception to the drive to provide improved quality and cost-effective patient care. Critical care practitioners are seen to have a specialised knowledge base, specific skills in delivering advanced health care and a commitment to serve the critically ill patient. It is expected of them to be aware of new and emerging evidence about health disease processes, treatment modalities and technology used in the critical-care units. Due to the dynamic nature of a critical care unit, it is essential that every practitioner working in the unit, whether a novice professional nurse or senior unit manager, needs to be aware of the current evidence guiding their practices (Elliot, Aitken, Chaboyer, 2007:18). With reference to the critically ill patient who is connected to a mechanical ventilator, practices related to the nursing care of this group of patients, who mostly occupy the critical care units, should be based on the best evidence in order to provide cost-effective and quality care. The research study aimed to explore and describe four identified nursing care practices related to safety of a mechanically ventilated patient as performed by professional nurses in the critical care units in the Nelson Mandela Metropole. The identified nursing-care practices include: endotrachael tube placement verification, endotracheal tube cuff pressure monitoring, endotrachael tube suctioning and mechanical ventilator settings. This objective was operationalized in Stage One of the study, by using a quantitative, explorative, descriptive and contextual approach. A structured questionnaire was utilised to collect data from professional nurses working in critical care units. From the analysed data, it was decided to select the two nursing care practices that were done least according to the best recommended practice, namely endotracheal tube suctioning and endotracheal tube cuff pressure monitoring. Based on the results, systematic reviews were done respectively on the two nursing care practices. On completion of Stage One of the study, evidence-informed clinical guidelines for the two identified nursing care practices were developed. The clinical guidelines were based on the evidence found in conducting the systematic reviews. The draft clinical guidelines were reviewed by an expert panel. Feedback from the reviewers was considered to prepare the final evidence-informed clinical guidelines. Based on the clinical guidelines, two clinical algorithms were developed, which might be used at the patient's bedside and can assist in quick dissemination of the recommendations for practice. Ethical considerations were maintained throughout the study. The quality of the study was ensured in applying the principles of validity and reliability as well as performing a critical appraisal of all data collected during the systematic review. It is envisaged that the study findings be disseminated in the critical care units in the Nelson Mandela Metropole and published in peer reviewed journals.
- Full Text:
- Date Issued: 2011
Examining educators’ strategies for promoting science learning and science literacy among grade 9 learners in a South African Rural Education District
- Authors: Mtsi, Nomxolisi
- Date: 2019
- Subjects: Science -- Study and teaching (Elementary) Children's literature in science education
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/16150 , vital:40673
- Description: Science learning and Science Literacy (SL) play a crucial role in preparing learners to participate in the country’s economy with the relevant knowledge, higher order thinking and analytical reasoning to solve day-to-day problems. The purpose of this research was to examine educators’ strategies for promoting science learning and SL among grade 9 learners in a rural education district in South Africa’s Eastern Cape Province. Learning of science and promotion of SL complement each other and therefore science educators’ strategies are pertinent. The study used pragmatic paradigm and the mixed method approach and was informed by cognitive and social constructivism as theoretical frameworks. For data collection, the study used convenience sampling based on the proximity and comparative ease of the researcher to reach the rural schools to select 30 out of 67 schools spread over six out of the eight circuits in the selected education district. On the other hand, purposive sampling was used for the selection of learners by their educators based on academic performance, top, average and below average achievers. Since each school had only one grade 9 science educator, 30 grade 9 science educators from the selected 30 schools formed the sample for quantitative data. Out of these 30, 10 educators who volunteered first for interviews and gave permission for being observed in their classrooms were selected for qualitative data collection. Three learners in each of the 10 schools from which the educators for qualitative data collection were selected, constituted the learners’ sample (30 learners). While data were collected through questionnaires, interviews and observations from educators, data from learners were collected through focus group (FG) interviews. The questionnaire was structured in order to gather educators’ biographical data as well as information on educators’ strategies to promote science learning and SL. The interview schedule was similar to the questionnaire but biographical data were excluded. Observations focused on educators’ strategies for science learning, SL and assessments. The data from the questionnaire were descriptively analysed and the qualitative and transcribed observation data were thematically analysed. Final conclusions were drawn based on the triangulated data. Major findings showed that the strategies which the educators employed in the descending order of use were: Investigation at 97percent; Discussion, Presentation and Project, each at 93percent; Problem solving at 90percent; Demonstration and Question-Answer, each at 87percent; Case study and Brainstorming, each at 77percent; Role-play at 63percent; Lecture at 57percent; Modelling at 47percent, Inquiry at 27percent and Simulation at 23percent. Findings also revealed the strengths and weaknesses of the strategies used by educators. Some of the strengths were: promotion of interactive learning; stimulation of research skills; enhancement of critical thinking and development of confidence through participation. Some of the weaknesses were: learners’ tendency to rely on others; lack of resources hindering learning and promotion of plagiarism. The study recommends that all stakeholders must work together to achieve good quality education. District and Provincial officers ought to track and monitor the science curriculum implementation. Subject specialists, educator subject committees and cluster leaders must also be active in strategic planning for enhancing SL in schools by putting forward their inputs and adopting consensus-based ones. A framework for driving science content with pedagogical content knowledge and practicals-driven strategy for enhancing science content knowledge is proposed.
- Full Text:
- Date Issued: 2019
- Authors: Mtsi, Nomxolisi
- Date: 2019
- Subjects: Science -- Study and teaching (Elementary) Children's literature in science education
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/16150 , vital:40673
- Description: Science learning and Science Literacy (SL) play a crucial role in preparing learners to participate in the country’s economy with the relevant knowledge, higher order thinking and analytical reasoning to solve day-to-day problems. The purpose of this research was to examine educators’ strategies for promoting science learning and SL among grade 9 learners in a rural education district in South Africa’s Eastern Cape Province. Learning of science and promotion of SL complement each other and therefore science educators’ strategies are pertinent. The study used pragmatic paradigm and the mixed method approach and was informed by cognitive and social constructivism as theoretical frameworks. For data collection, the study used convenience sampling based on the proximity and comparative ease of the researcher to reach the rural schools to select 30 out of 67 schools spread over six out of the eight circuits in the selected education district. On the other hand, purposive sampling was used for the selection of learners by their educators based on academic performance, top, average and below average achievers. Since each school had only one grade 9 science educator, 30 grade 9 science educators from the selected 30 schools formed the sample for quantitative data. Out of these 30, 10 educators who volunteered first for interviews and gave permission for being observed in their classrooms were selected for qualitative data collection. Three learners in each of the 10 schools from which the educators for qualitative data collection were selected, constituted the learners’ sample (30 learners). While data were collected through questionnaires, interviews and observations from educators, data from learners were collected through focus group (FG) interviews. The questionnaire was structured in order to gather educators’ biographical data as well as information on educators’ strategies to promote science learning and SL. The interview schedule was similar to the questionnaire but biographical data were excluded. Observations focused on educators’ strategies for science learning, SL and assessments. The data from the questionnaire were descriptively analysed and the qualitative and transcribed observation data were thematically analysed. Final conclusions were drawn based on the triangulated data. Major findings showed that the strategies which the educators employed in the descending order of use were: Investigation at 97percent; Discussion, Presentation and Project, each at 93percent; Problem solving at 90percent; Demonstration and Question-Answer, each at 87percent; Case study and Brainstorming, each at 77percent; Role-play at 63percent; Lecture at 57percent; Modelling at 47percent, Inquiry at 27percent and Simulation at 23percent. Findings also revealed the strengths and weaknesses of the strategies used by educators. Some of the strengths were: promotion of interactive learning; stimulation of research skills; enhancement of critical thinking and development of confidence through participation. Some of the weaknesses were: learners’ tendency to rely on others; lack of resources hindering learning and promotion of plagiarism. The study recommends that all stakeholders must work together to achieve good quality education. District and Provincial officers ought to track and monitor the science curriculum implementation. Subject specialists, educator subject committees and cluster leaders must also be active in strategic planning for enhancing SL in schools by putting forward their inputs and adopting consensus-based ones. A framework for driving science content with pedagogical content knowledge and practicals-driven strategy for enhancing science content knowledge is proposed.
- Full Text:
- Date Issued: 2019
Examining the teaching of natural sciences concepts to English second language speakers in selected primary schools in the East London Education District
- Authors: Kumanda, Nomaroma
- Date: 2019
- Subjects: Science -- Study and teaching
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/15925 , vital:40564
- Description: The study sought to examine the teaching of Natural Sciences (NS) concepts to English second language speakers in selected primary schools. Grade 7 was chosen for the investigation as it precedes the last class at primary school. The researcher investigated the teaching and learning of Natural sciences concepts. A mixed method design was used. The research questions focused on the teaching of NS, learner-centred strategies used, challenges encountered, strategies employed by teachers when teaching NS, and the implications for science education.The purpose was to examine the teaching of Natural Sciences concepts to English second language speakers in selected primary schools in the East London Education District. Data were collected from grade 7 teachers and learners in selected township schools in East London, by means of semi-structured interviews, questionnaires and focus groups. Semi-structured questionnaires were administered to teachers and learners, individual interviews were conducted with NS head of department, focus group and interviews were held with grade 7 NS learners. Quantitative and qualitative data were independently analysed and merged at the interpretation stage for triangulation of results. The questions were grouped according to their themes. The researcher’s findings revealed that teachers linked the teaching of NS concepts by showing the teaching skills. Teachers used code-switching for learners to understand the scientific concepts for positive attitude to improve the learner performance. Teachers experienced problems using English as a Language of Learning and Teaching (LoLT) while teaching NS concepts, teachers’ understanding of NS influenced their ability to implement of transformational curriculum, but learners did not benefit much when learning science in English Language and those policies were designed by the Department of Education without teacher involvement. The study concluded by noting that It emerged that teachers and learners experienced difficulties in teaching and learning of the NS concepts.
- Full Text:
- Date Issued: 2019
- Authors: Kumanda, Nomaroma
- Date: 2019
- Subjects: Science -- Study and teaching
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/15925 , vital:40564
- Description: The study sought to examine the teaching of Natural Sciences (NS) concepts to English second language speakers in selected primary schools. Grade 7 was chosen for the investigation as it precedes the last class at primary school. The researcher investigated the teaching and learning of Natural sciences concepts. A mixed method design was used. The research questions focused on the teaching of NS, learner-centred strategies used, challenges encountered, strategies employed by teachers when teaching NS, and the implications for science education.The purpose was to examine the teaching of Natural Sciences concepts to English second language speakers in selected primary schools in the East London Education District. Data were collected from grade 7 teachers and learners in selected township schools in East London, by means of semi-structured interviews, questionnaires and focus groups. Semi-structured questionnaires were administered to teachers and learners, individual interviews were conducted with NS head of department, focus group and interviews were held with grade 7 NS learners. Quantitative and qualitative data were independently analysed and merged at the interpretation stage for triangulation of results. The questions were grouped according to their themes. The researcher’s findings revealed that teachers linked the teaching of NS concepts by showing the teaching skills. Teachers used code-switching for learners to understand the scientific concepts for positive attitude to improve the learner performance. Teachers experienced problems using English as a Language of Learning and Teaching (LoLT) while teaching NS concepts, teachers’ understanding of NS influenced their ability to implement of transformational curriculum, but learners did not benefit much when learning science in English Language and those policies were designed by the Department of Education without teacher involvement. The study concluded by noting that It emerged that teachers and learners experienced difficulties in teaching and learning of the NS concepts.
- Full Text:
- Date Issued: 2019
Experiential learning in an undergraduate BPHARM programme: impact of an intervention on academic achievement
- Authors: McCartney, Jane Alison
- Date: 2017
- Subjects: Pharmacy -- Study and teaching -- South Africa Experiential learning -- South Africa , Academic achievement -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19037 , vital:28767
- Description: The extended role of today’s pharmacist with the emphasis on patient-focused care has highlighted the need for increased exposure of undergraduate pharmacy students to experiential learning in patient-centred environments, and additional skills development in therapeutics, problem solving and clinical decision making. At the Nelson Mandela Metropolitan University (NMMU), final year pharmacy students complete a university-coordinated, hospital-based, structured experiential learning programme (ELP) for the exit-level module, Pharmacology4. However, the students consistently experience difficulties in the application of pharmacological knowledge during the transition from lecture-based learning to the patient-focused clinical setting. The student population at the NMMU is diverse, with varied cultural, ethnic, language and secondary level education backgrounds, as well as different learning preferences and approaches. The extent to which these factors affect academic achievement in the experiential learning environment is unknown. Central research question The central research question for this study was therefore, “What would be the effect of an intervention aimed at supporting undergraduate pharmacy students during clinical placements, on academic achievement in, and student attitudes towards, experiential learning programmes (ELP)?” In order to explore the research question, several factors which may influence academic achievement in ELPs were investigated, namely: academic achievement (pre-university, in the BPharm programme and, in pharmacology); the admission route into the BPharm programme and the subsequent rate of academic progression; English reading comprehension ability; learning styles; problem solving ability; the extent to which students are prepared for application of knowledge in the ELP, in terms of assessment methods used prior to the final year and previous pharmacy work-based experience. In addition, the students’ lived experience of the ELP was explored, and the need for, and nature of, an intervention was determined. The research was based in a pragmatic paradigm, using an advanced mixed methods approach. An intervention-based, two-phase, quasi-experimental design was employed with an initial exploratory Preliminary Phase (in 2013) preceding the larger experimental framework (Phases One and Two, in 2014 and 2015 respectively). The research design was primarily quantitative, with pre- and post- testing conducted before and after the ELP. The ELP was completed by the comparator cohort in Phase One and the experimental cohort in Phase Two. Supplementary qualitative data was collected before, during and after the ELP. The intervention, in the form of supplementary academic support sessions, was developed from the qualitative data using an iterative approach, and implemented during the ELP in Phase Two. Attitudes and expectations of the students towards the hospital-based ELP were generally positive and realistic. Areas of concern included the difficulties experienced in the application and integration of pharmacological knowledge, both in the clinical setting and the clinical case study-based assessments; students feeling overwhelmed, inadequate and inferior in the clinical environment, compounded by an absence of clinical pharmacists as role models; and feeling unprepared for patient-focused care. The qualitative data strongly supported the need for supplementary academic support sessions. The intervention was developed and implemented in Phase Two, using patient case-based, active learning strategies. The majority of students (91.0%; n = 104) reported improved case analysis skills. A statistically significant (p = .030, Cohen’s d = 0.34) improvement was noted in the summative Pharmacology4 assessment marks obtained by the experimental cohort post-intervention, although of small practical significance. Predictors of academic achievement in the ELP were found to be language, specifically English reading comprehension skills, academic achievement in the BPharm programme and pharmacology, the university admission score, the rate of academic progression, and problem solving ability. Previous pharmacy-based work experience and assessment questions requiring application of knowledge were also found to influence achievement in the ELP. The need for an intervention in the form of supplementary academic support sessions was confirmed. The intervention was subsequently developed and successfully implemented, with student-reported self-perceived improvements in patient case analysis skills. These positive findings were supported by quantitative data which showed a statistically significant improvement in academic achievement in the ELP. Several predictors of academic achievement in the ELP were identified, and invaluable insight was gained into the nature of the difficulties experienced by pharmacy students in the transition from lecture-based learning to experiential learning in patient-focused environments.
- Full Text:
- Date Issued: 2017
- Authors: McCartney, Jane Alison
- Date: 2017
- Subjects: Pharmacy -- Study and teaching -- South Africa Experiential learning -- South Africa , Academic achievement -- South Africa
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/19037 , vital:28767
- Description: The extended role of today’s pharmacist with the emphasis on patient-focused care has highlighted the need for increased exposure of undergraduate pharmacy students to experiential learning in patient-centred environments, and additional skills development in therapeutics, problem solving and clinical decision making. At the Nelson Mandela Metropolitan University (NMMU), final year pharmacy students complete a university-coordinated, hospital-based, structured experiential learning programme (ELP) for the exit-level module, Pharmacology4. However, the students consistently experience difficulties in the application of pharmacological knowledge during the transition from lecture-based learning to the patient-focused clinical setting. The student population at the NMMU is diverse, with varied cultural, ethnic, language and secondary level education backgrounds, as well as different learning preferences and approaches. The extent to which these factors affect academic achievement in the experiential learning environment is unknown. Central research question The central research question for this study was therefore, “What would be the effect of an intervention aimed at supporting undergraduate pharmacy students during clinical placements, on academic achievement in, and student attitudes towards, experiential learning programmes (ELP)?” In order to explore the research question, several factors which may influence academic achievement in ELPs were investigated, namely: academic achievement (pre-university, in the BPharm programme and, in pharmacology); the admission route into the BPharm programme and the subsequent rate of academic progression; English reading comprehension ability; learning styles; problem solving ability; the extent to which students are prepared for application of knowledge in the ELP, in terms of assessment methods used prior to the final year and previous pharmacy work-based experience. In addition, the students’ lived experience of the ELP was explored, and the need for, and nature of, an intervention was determined. The research was based in a pragmatic paradigm, using an advanced mixed methods approach. An intervention-based, two-phase, quasi-experimental design was employed with an initial exploratory Preliminary Phase (in 2013) preceding the larger experimental framework (Phases One and Two, in 2014 and 2015 respectively). The research design was primarily quantitative, with pre- and post- testing conducted before and after the ELP. The ELP was completed by the comparator cohort in Phase One and the experimental cohort in Phase Two. Supplementary qualitative data was collected before, during and after the ELP. The intervention, in the form of supplementary academic support sessions, was developed from the qualitative data using an iterative approach, and implemented during the ELP in Phase Two. Attitudes and expectations of the students towards the hospital-based ELP were generally positive and realistic. Areas of concern included the difficulties experienced in the application and integration of pharmacological knowledge, both in the clinical setting and the clinical case study-based assessments; students feeling overwhelmed, inadequate and inferior in the clinical environment, compounded by an absence of clinical pharmacists as role models; and feeling unprepared for patient-focused care. The qualitative data strongly supported the need for supplementary academic support sessions. The intervention was developed and implemented in Phase Two, using patient case-based, active learning strategies. The majority of students (91.0%; n = 104) reported improved case analysis skills. A statistically significant (p = .030, Cohen’s d = 0.34) improvement was noted in the summative Pharmacology4 assessment marks obtained by the experimental cohort post-intervention, although of small practical significance. Predictors of academic achievement in the ELP were found to be language, specifically English reading comprehension skills, academic achievement in the BPharm programme and pharmacology, the university admission score, the rate of academic progression, and problem solving ability. Previous pharmacy-based work experience and assessment questions requiring application of knowledge were also found to influence achievement in the ELP. The need for an intervention in the form of supplementary academic support sessions was confirmed. The intervention was subsequently developed and successfully implemented, with student-reported self-perceived improvements in patient case analysis skills. These positive findings were supported by quantitative data which showed a statistically significant improvement in academic achievement in the ELP. Several predictors of academic achievement in the ELP were identified, and invaluable insight was gained into the nature of the difficulties experienced by pharmacy students in the transition from lecture-based learning to experiential learning in patient-focused environments.
- Full Text:
- Date Issued: 2017
Exploration of Nahoon beach milieu for lignocellulose degrading bacteria and optimizing fermentation conditions for holocellulase production by selected strains
- Authors: Fatokun, Evelyn
- Date: 2016
- Subjects: Lignocellulose Lignocellulose -- Biodegradation Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1529 , vital:27413
- Description: A significant trend in the modern day industrial biotechnology is the utilization and application of renewable resources, and ecofriendly approach to industrial processes and waste management. As a consequence thereof, the biotechnology of holocellulases: cellulase and xylanase and, enzymatic hydrolysis of renewable and abundant lignocellulosic biomass to energy and value added products are rapidly increasing; hence, cost effective enzyme system is imperative. In that context, exploration of microbiota for strains and enzymes with novel industrial properties is vital for efficient and commercially viable enzyme biotechnology. Consequent on the complex characteristics of high salinity, variable pressure, temperature and nutritional conditions, bacterial strains from the marine environment are equipped with enzyme machinery of industrial importance for adaptation and survival. In this study, bacterial strains were isolated form Nahoon beach and optimized for holocellulase production. Three isolates selected for lignocellulolytic potential were identified by 16S ribosomal deoxyribonucleic acid (rDNA) sequence analysis. Isolate FS1k had 98 percent similarity with Streptomyces albidoflavus strain AIH12, was designated as Streptomyces albidoflavus strain SAMRC-UFH5 and deposited in the GenBank with accession number KU171373. Similarly, isolates CS14b and CS22d with respective percentage similarity of 98 and 99 (percent) with Bacillus cereus strains and Streptomyces sp. strain WMMB251 were named Bacillus cereus strain SAMRC-UFH9 and Streptomyces sp. strain SAMRC-UFH6; and were deposited in the GenBank with accession number KX524510 and KU171374 respectively. Optimal pH, temperature and agitation speed for cellulase production by S. albidoflavus strain SAMRC-UFH5, and B. cereus strain SAMRC-UFH9 were 6 and 7; 40 and 30 (°C); and 100 and 150 (rpm) respectively; while xylanase production was optimal at pH, temperature and agitation speed of 8 and 7; 40 and 30 (°C); and 150 and 50 (rpm) respectively. Maximum cellulase activity of 0.26 and 0.061(U/mL) by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 were attained at 60 h respectively, while maximal xylanase activity of 18.54 and 16.6 (U/mL) was produced by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 at 48 h and 60 h respectively. Furthermore, xylanase production by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 was maximally induced by wheat straw and xylan respectively, while cellulase production was best induced by mannose and carboxymethyl cellulose respectively. On the other hand, cellulase and xylanase production by Streptomyces sp. strain SAMRC-UFH6 was optimal at pH, temperature and agitation speed of 7 and 8, 40 °C and 100 rpm, respectively. Highest production of cellulase and xylanase was attained at 84 and 60 h with respective activity of 0.065 and 6.34 (U/mL). In addition, cellulase and xylanase production by the strain was best induced by beechwood xylan. Moreover, xylanase produced by Streptomyces sp. strain SAMRC-UFH6 at optimal conditions was characterized by optimal pH and temperature of 8 and 80-90 °C respectively; retaining over 70 percent activity at pH 5-10 after 1 h and 60 percent of initial activity at 90 °C after 90 min of incubation. In all, optimization improved cellulase and xylanase production yields, being 40 and 95.5, 10.89 and 72.17, and 10 and 115- fold increase by S. albidoflavus strain SAMRC-UFH5, B. cereus strain SAMRC-UFH9 and Streptomyces sp. SAMRC-UFH6 respectively. The results of this study suggest that the marine bacterial strains are resource for holocellulase with industrial applications.
- Full Text:
- Date Issued: 2016
- Authors: Fatokun, Evelyn
- Date: 2016
- Subjects: Lignocellulose Lignocellulose -- Biodegradation Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1529 , vital:27413
- Description: A significant trend in the modern day industrial biotechnology is the utilization and application of renewable resources, and ecofriendly approach to industrial processes and waste management. As a consequence thereof, the biotechnology of holocellulases: cellulase and xylanase and, enzymatic hydrolysis of renewable and abundant lignocellulosic biomass to energy and value added products are rapidly increasing; hence, cost effective enzyme system is imperative. In that context, exploration of microbiota for strains and enzymes with novel industrial properties is vital for efficient and commercially viable enzyme biotechnology. Consequent on the complex characteristics of high salinity, variable pressure, temperature and nutritional conditions, bacterial strains from the marine environment are equipped with enzyme machinery of industrial importance for adaptation and survival. In this study, bacterial strains were isolated form Nahoon beach and optimized for holocellulase production. Three isolates selected for lignocellulolytic potential were identified by 16S ribosomal deoxyribonucleic acid (rDNA) sequence analysis. Isolate FS1k had 98 percent similarity with Streptomyces albidoflavus strain AIH12, was designated as Streptomyces albidoflavus strain SAMRC-UFH5 and deposited in the GenBank with accession number KU171373. Similarly, isolates CS14b and CS22d with respective percentage similarity of 98 and 99 (percent) with Bacillus cereus strains and Streptomyces sp. strain WMMB251 were named Bacillus cereus strain SAMRC-UFH9 and Streptomyces sp. strain SAMRC-UFH6; and were deposited in the GenBank with accession number KX524510 and KU171374 respectively. Optimal pH, temperature and agitation speed for cellulase production by S. albidoflavus strain SAMRC-UFH5, and B. cereus strain SAMRC-UFH9 were 6 and 7; 40 and 30 (°C); and 100 and 150 (rpm) respectively; while xylanase production was optimal at pH, temperature and agitation speed of 8 and 7; 40 and 30 (°C); and 150 and 50 (rpm) respectively. Maximum cellulase activity of 0.26 and 0.061(U/mL) by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 were attained at 60 h respectively, while maximal xylanase activity of 18.54 and 16.6 (U/mL) was produced by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 at 48 h and 60 h respectively. Furthermore, xylanase production by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 was maximally induced by wheat straw and xylan respectively, while cellulase production was best induced by mannose and carboxymethyl cellulose respectively. On the other hand, cellulase and xylanase production by Streptomyces sp. strain SAMRC-UFH6 was optimal at pH, temperature and agitation speed of 7 and 8, 40 °C and 100 rpm, respectively. Highest production of cellulase and xylanase was attained at 84 and 60 h with respective activity of 0.065 and 6.34 (U/mL). In addition, cellulase and xylanase production by the strain was best induced by beechwood xylan. Moreover, xylanase produced by Streptomyces sp. strain SAMRC-UFH6 at optimal conditions was characterized by optimal pH and temperature of 8 and 80-90 °C respectively; retaining over 70 percent activity at pH 5-10 after 1 h and 60 percent of initial activity at 90 °C after 90 min of incubation. In all, optimization improved cellulase and xylanase production yields, being 40 and 95.5, 10.89 and 72.17, and 10 and 115- fold increase by S. albidoflavus strain SAMRC-UFH5, B. cereus strain SAMRC-UFH9 and Streptomyces sp. SAMRC-UFH6 respectively. The results of this study suggest that the marine bacterial strains are resource for holocellulase with industrial applications.
- Full Text:
- Date Issued: 2016