Photodynamic activity of Sn (IV) tetrathien-2-ylchlorin against MCF-7 breast cancer cells
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185920 , vital:44448 , xlink:href="https://doi.org/10.1039/d0dt03958f"
- Description: A new readily-synthesized Sn(IV) tetraarylchlorin with thien-2-yl substituents (SnC) has been prepared and fully characterized by various spectroscopic techniques and its photophysical and photochemical properties, such as the singlet oxygen quantum yield (ΦΔ), fluorescence quantum yield (ΦF), triplet lifetime (τT) and photostability, have been evaluated. SnC has an unusually high ΦΔ value of 0.89 in DMF. Studies on the photodynamic activity against MCF-7 breast cancer cells exhibited a very low IC50 value of 0.9 μM and high phototoxicity (dark versus light) indices of >27.8 after irradiation with a 660 nm Thorlabs LED (280 mW cm−2). The results demonstrate that Sn(IV) tetraarylchlorins of this type are suitable candidates for further in-depth PDT studies.
- Full Text:
- Date Issued: 2021
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185920 , vital:44448 , xlink:href="https://doi.org/10.1039/d0dt03958f"
- Description: A new readily-synthesized Sn(IV) tetraarylchlorin with thien-2-yl substituents (SnC) has been prepared and fully characterized by various spectroscopic techniques and its photophysical and photochemical properties, such as the singlet oxygen quantum yield (ΦΔ), fluorescence quantum yield (ΦF), triplet lifetime (τT) and photostability, have been evaluated. SnC has an unusually high ΦΔ value of 0.89 in DMF. Studies on the photodynamic activity against MCF-7 breast cancer cells exhibited a very low IC50 value of 0.9 μM and high phototoxicity (dark versus light) indices of >27.8 after irradiation with a 660 nm Thorlabs LED (280 mW cm−2). The results demonstrate that Sn(IV) tetraarylchlorins of this type are suitable candidates for further in-depth PDT studies.
- Full Text:
- Date Issued: 2021
Naked Eye and Colorimetric Detection of Cyanide with a 1, 3‐Diethyl‐2‐thiobarbituric Acid Substituted Ferrocene Chemosensor
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190567 , vital:45006 , xlink:href="https://doi.org/10.1002/slct.202100163"
- Description: A 1,3-diethyl-2-thiobarbituric-acid-substituted ferrocene (FET) has been evaluated for its cyanide sensing ability by UV-visible absorption spectroscopy and other characterization methods. FET provides a ratiometric colorimetric chemosensor for the CN− anion detection in 1 : 1 DMSO/H2O (v/v) solution. The addition of CN− results in an immediate color change from dark blue to pale orange that is visible to the naked eye. Mechanism studies and molecular modelling with TD-DFT calculations demonstrate that nucleophilic addition of CN− to an electrophilic sp2-hybridized carbon atom blocks charge transfer from the ferrocene ring complex to the thiobarbituric acid moiety. The FET sensor exhibits excellent selectivity for CN− and a limit of detection of 0.2 μM.
- Full Text:
- Date Issued: 2021
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190567 , vital:45006 , xlink:href="https://doi.org/10.1002/slct.202100163"
- Description: A 1,3-diethyl-2-thiobarbituric-acid-substituted ferrocene (FET) has been evaluated for its cyanide sensing ability by UV-visible absorption spectroscopy and other characterization methods. FET provides a ratiometric colorimetric chemosensor for the CN− anion detection in 1 : 1 DMSO/H2O (v/v) solution. The addition of CN− results in an immediate color change from dark blue to pale orange that is visible to the naked eye. Mechanism studies and molecular modelling with TD-DFT calculations demonstrate that nucleophilic addition of CN− to an electrophilic sp2-hybridized carbon atom blocks charge transfer from the ferrocene ring complex to the thiobarbituric acid moiety. The FET sensor exhibits excellent selectivity for CN− and a limit of detection of 0.2 μM.
- Full Text:
- Date Issued: 2021
Photocytotoxicity of heavy-atom-free thiobarbituric acid functionalized pyrene derivatives against MCF-7 cancer cells
- Babu, Balaji, Ali, Thaslima A, Ochappan, Thivagar, Mack, John, Nyokong, Tebello, Sethuraman, Mathur G
- Authors: Babu, Balaji , Ali, Thaslima A , Ochappan, Thivagar , Mack, John , Nyokong, Tebello , Sethuraman, Mathur G
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190556 , vital:45005 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.102102"
- Description: Two thiobarbituric acid-functionalized pyrene derivatives (P1, P2) have been synthesized to explore the photophysical properties and photodynamic activity of dyes of this type. Both compounds exhibit an intense intramolecular charge transfer (ICT) band at ca. 470 nm, which is absent in the spectra of the precursor. P1 and P2 exhibit singlet oxygen generation on irradiation with light with moderate singlet oxygen yields of 0.36 and 0.32, respectively, in DMSO. P1 showed better photodynamic activity against MCF-7 cells with an IC50 value of 18.3 μM under illumination at 455 nm for 60 min with a Thorlabs M455L3 LED (330 mW.cm−2).
- Full Text:
- Date Issued: 2021
- Authors: Babu, Balaji , Ali, Thaslima A , Ochappan, Thivagar , Mack, John , Nyokong, Tebello , Sethuraman, Mathur G
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190556 , vital:45005 , xlink:href="https://doi.org/10.1016/j.pdpdt.2020.102102"
- Description: Two thiobarbituric acid-functionalized pyrene derivatives (P1, P2) have been synthesized to explore the photophysical properties and photodynamic activity of dyes of this type. Both compounds exhibit an intense intramolecular charge transfer (ICT) band at ca. 470 nm, which is absent in the spectra of the precursor. P1 and P2 exhibit singlet oxygen generation on irradiation with light with moderate singlet oxygen yields of 0.36 and 0.32, respectively, in DMSO. P1 showed better photodynamic activity against MCF-7 cells with an IC50 value of 18.3 μM under illumination at 455 nm for 60 min with a Thorlabs M455L3 LED (330 mW.cm−2).
- Full Text:
- Date Issued: 2021
A heavy-atom-free π-extended N-confused porphyrin as a photosensitizer for photodynamic therapy
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185909 , vital:44447 , xlink:href="https://doi.org/10.1039/d1nj00112d"
- Description: The synthesis and characterization of a novel 1,3-diethyl-2-thiobarbituric-acid-substituted N-confused porphyrin (NCP-TB) is reported, along with a study of its photodynamic activity against MCF-7 cells using 530 (110 mW cm−2) and 660 nm (280 mW cm−2) Thorlabs light-emitting diodes for 30 min. The singlet oxygen quantum yield for NCP-TB is 0.38 compared to 0.23 for the parent unsubstituted N-confused porphyrin (NCP) due to the presence of a sulfur atom. NCP-TB exhibits enhanced PDT activity compared to NCP at both wavelengths. A significantly lower IC50 value of 5.2 μM was obtained at 530 nm (14.7 μM at 660 nm) despite a smaller light dose, due to a large red shift of the intense B band into the green region of the spectrum. 2′,7′-Dichlorofluorescein diacetate (DCFDA) assays demonstrate that there is intracellular generation of reactive oxygen species upon exposure to light.
- Full Text:
- Date Issued: 2021
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/185909 , vital:44447 , xlink:href="https://doi.org/10.1039/d1nj00112d"
- Description: The synthesis and characterization of a novel 1,3-diethyl-2-thiobarbituric-acid-substituted N-confused porphyrin (NCP-TB) is reported, along with a study of its photodynamic activity against MCF-7 cells using 530 (110 mW cm−2) and 660 nm (280 mW cm−2) Thorlabs light-emitting diodes for 30 min. The singlet oxygen quantum yield for NCP-TB is 0.38 compared to 0.23 for the parent unsubstituted N-confused porphyrin (NCP) due to the presence of a sulfur atom. NCP-TB exhibits enhanced PDT activity compared to NCP at both wavelengths. A significantly lower IC50 value of 5.2 μM was obtained at 530 nm (14.7 μM at 660 nm) despite a smaller light dose, due to a large red shift of the intense B band into the green region of the spectrum. 2′,7′-Dichlorofluorescein diacetate (DCFDA) assays demonstrate that there is intracellular generation of reactive oxygen species upon exposure to light.
- Full Text:
- Date Issued: 2021
A Sn (iv) porphyrin with mitochondria targeting properties for enhanced photodynamic activity against MCF-7 cells
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2022
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/230897 , vital:49829 , xlink:href="https://doi.org/10.1039/D2NJ00350C"
- Description: Two readily synthesized Sn(IV) porphyrins (SnP, SnPH) have been prepared with and without the cationic triphenylphosphonium moiety (TPP+), which have high singlet oxygen quantum yields (ca. 0.72) and long triplet state lifetimes. The Sn(IV) porphyrin with a TPP+ moiety (SnPH) exhibits favorable photodynamic activity against MCF-7 cells with IC50 values of 2.9 μM. SnPH exhibits higher cellular uptake than SnP in MCF-7 cells. A Rhodamine 123 (Rh123) assay showed that SnPH targets mitochondria and induces apoptosis by generating reactive oxygen species. The results demonstrate that this structural modification strategy merits further in-depth study.
- Full Text:
- Date Issued: 2022
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2022
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/230897 , vital:49829 , xlink:href="https://doi.org/10.1039/D2NJ00350C"
- Description: Two readily synthesized Sn(IV) porphyrins (SnP, SnPH) have been prepared with and without the cationic triphenylphosphonium moiety (TPP+), which have high singlet oxygen quantum yields (ca. 0.72) and long triplet state lifetimes. The Sn(IV) porphyrin with a TPP+ moiety (SnPH) exhibits favorable photodynamic activity against MCF-7 cells with IC50 values of 2.9 μM. SnPH exhibits higher cellular uptake than SnP in MCF-7 cells. A Rhodamine 123 (Rh123) assay showed that SnPH targets mitochondria and induces apoptosis by generating reactive oxygen species. The results demonstrate that this structural modification strategy merits further in-depth study.
- Full Text:
- Date Issued: 2022
An octabrominated Sn (iv) tetraisopropylporphyrin as a photosensitizer dye for singlet oxygen biomedical applications
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186679 , vital:44524 , xlink:href="https://doi.org/10.1039/D0DT01915A"
- Description: Two novel Sn(IV) tetraisopropylphenylporphyrins have been synthesized to explore the effect of octabromination at the β-pyrrole positions on their photophysical properties and photodynamic activity. The lower energy Q band of an octabrominated complex lies at 675 nm well within the therapeutic window. The octabrominated dye has a relatively high singlet oxygen quantum yield of 0.78 in DMF and exhibits favorable photodynamic activity against MCF-7 cells with an IC50 value of 10.7 μM and a 5.74 log reduction value (5 μM) towards S. aureus under illumination at 660 nm for 60 min with a Thorlabs M660L3 LED (280 mW cm−2).
- Full Text:
- Date Issued: 2020
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186679 , vital:44524 , xlink:href="https://doi.org/10.1039/D0DT01915A"
- Description: Two novel Sn(IV) tetraisopropylphenylporphyrins have been synthesized to explore the effect of octabromination at the β-pyrrole positions on their photophysical properties and photodynamic activity. The lower energy Q band of an octabrominated complex lies at 675 nm well within the therapeutic window. The octabrominated dye has a relatively high singlet oxygen quantum yield of 0.78 in DMF and exhibits favorable photodynamic activity against MCF-7 cells with an IC50 value of 10.7 μM and a 5.74 log reduction value (5 μM) towards S. aureus under illumination at 660 nm for 60 min with a Thorlabs M660L3 LED (280 mW cm−2).
- Full Text:
- Date Issued: 2020
Sn (iv) N-confused porphyrins as photosensitizer dyes for photodynamic therapy in the near IR region
- Babu, Balaji, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186648 , vital:44521 , xlink:href="https://doi.org/10.1039/D0DT03296D"
- Description: The utility of Sn(IV) N-confused porphyrin (SnNCP) for use as photosensitizer dyes in photodynamic therapy is investigated. SnNCP has an unusually high singlet oxygen quantum yield of 0.72 in DMSO. IC50 values of 1.6 and 12.8 μM were obtained against MCF-7 cells upon irradiation with 660 and 780 nm LEDs.
- Full Text:
- Date Issued: 2020
Sn (iv) N-confused porphyrins as photosensitizer dyes for photodynamic therapy in the near IR region
- Authors: Babu, Balaji , Mack, John , Nyokong, Tebello
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/186648 , vital:44521 , xlink:href="https://doi.org/10.1039/D0DT03296D"
- Description: The utility of Sn(IV) N-confused porphyrin (SnNCP) for use as photosensitizer dyes in photodynamic therapy is investigated. SnNCP has an unusually high singlet oxygen quantum yield of 0.72 in DMSO. IC50 values of 1.6 and 12.8 μM were obtained against MCF-7 cells upon irradiation with 660 and 780 nm LEDs.
- Full Text:
- Date Issued: 2020
Preparation of NIR absorbing axial substituted tin (iv) porphyrins and their photocytotoxic properties
- Babu, Balaji, Amuhaya, Edith K, Oluwole, David O, Prinsloo, Earl, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Amuhaya, Edith K , Oluwole, David O , Prinsloo, Earl , Mack, John , Nyokong, Tebello
- Date: 2019
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/234592 , vital:50211 , xlink:href="https://doi.org/10.1039/C8MD00373D"
- Description: Sn(IV) porphyrins ([Sn(IV)TTP(3PyO)2] (5) and [Sn(IV)TPP(3PyO)2] (6) [tetrathienylporphyrin (TTP), tetraphenylporphyrin (TPP), and pyridyloxy (PyO)]) were prepared and characterized and their photocytotoxicity upon irradiation with 625 nm light has been studied. The presence of the 3PyO axial ligands was found to limit the aggregation and enhance the solubility of 5 and 6 in DMF/H2O (1 : 1). The photophysical properties and photodynamic therapy (PDT) activity of the meso-2-thienyl and meso-phenyl-substituted Sn(IV) porphyrins are compared. 5 and 6 were found to be photocytotoxic in MCF-7 cancer cells when irradiated with a Thorlabs M625L3 LED at 625 nm but remained nontoxic in the dark. The PDT activity of Sn(IV) meso-tetra-2-thienylporphyrin 5 was found to be significantly enhanced relative to its analogous tetraphenylporphyrin 6. There is a marked red-shift of the Q00 band of 5 into the therapeutic window due to the meso-2-thienyl rings, and 5 has an unusually high singlet oxygen quantum yield value of 0.83 in DMF. The results demonstrate that readily synthesized axially ligated Sn(IV) meso-arylporphyrins are potentially suitable for use as singlet oxygen photosensitizers in biomedical applications and merit further in depth investigation in this context.
- Full Text:
- Date Issued: 2019
- Authors: Babu, Balaji , Amuhaya, Edith K , Oluwole, David O , Prinsloo, Earl , Mack, John , Nyokong, Tebello
- Date: 2019
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/234592 , vital:50211 , xlink:href="https://doi.org/10.1039/C8MD00373D"
- Description: Sn(IV) porphyrins ([Sn(IV)TTP(3PyO)2] (5) and [Sn(IV)TPP(3PyO)2] (6) [tetrathienylporphyrin (TTP), tetraphenylporphyrin (TPP), and pyridyloxy (PyO)]) were prepared and characterized and their photocytotoxicity upon irradiation with 625 nm light has been studied. The presence of the 3PyO axial ligands was found to limit the aggregation and enhance the solubility of 5 and 6 in DMF/H2O (1 : 1). The photophysical properties and photodynamic therapy (PDT) activity of the meso-2-thienyl and meso-phenyl-substituted Sn(IV) porphyrins are compared. 5 and 6 were found to be photocytotoxic in MCF-7 cancer cells when irradiated with a Thorlabs M625L3 LED at 625 nm but remained nontoxic in the dark. The PDT activity of Sn(IV) meso-tetra-2-thienylporphyrin 5 was found to be significantly enhanced relative to its analogous tetraphenylporphyrin 6. There is a marked red-shift of the Q00 band of 5 into the therapeutic window due to the meso-2-thienyl rings, and 5 has an unusually high singlet oxygen quantum yield value of 0.83 in DMF. The results demonstrate that readily synthesized axially ligated Sn(IV) meso-arylporphyrins are potentially suitable for use as singlet oxygen photosensitizers in biomedical applications and merit further in depth investigation in this context.
- Full Text:
- Date Issued: 2019
Synthesis, characterization and photodynamic activity of Sn (iv) triarylcorroles with red-shifted Q bands
- Babu, Balaji, Prinsloo, Earl, Mack, John, Nyokong, Tebello
- Authors: Babu, Balaji , Prinsloo, Earl , Mack, John , Nyokong, Tebello
- Date: 2019
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187521 , vital:44668 , xlink:href="https://doi.org/10.1039/C9NJ03391B"
- Description: Two Sn(IV) triarylcorroles were synthesised and characterized. The absorption spectrum of a meso-thien-2-yl substituted tin(IV)corrole (1-Sn) is red shifted compared to its phenyl analogue (2-Sn) and shows no sign of aggregation in solution. 1-Sn and 2-Sn exhibited singlet oxygen quantum yields of 0.87 and 0.54 in DMF, and have a triplet lifetime of 31 and 50 μs, respectively. Time dependent cellular uptake in MCF-7 cells for 1-Sn reached a peak at 24 h, and 1-Sn was found to be more lipophilic than 2-Sn. 1-Sn showed good photo-cytotoxicity on exposure to a Thorlabs 625 nm LED with an IC50 value of 3.2 μM and remained inactive in the dark.
- Full Text:
- Date Issued: 2019
- Authors: Babu, Balaji , Prinsloo, Earl , Mack, John , Nyokong, Tebello
- Date: 2019
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187521 , vital:44668 , xlink:href="https://doi.org/10.1039/C9NJ03391B"
- Description: Two Sn(IV) triarylcorroles were synthesised and characterized. The absorption spectrum of a meso-thien-2-yl substituted tin(IV)corrole (1-Sn) is red shifted compared to its phenyl analogue (2-Sn) and shows no sign of aggregation in solution. 1-Sn and 2-Sn exhibited singlet oxygen quantum yields of 0.87 and 0.54 in DMF, and have a triplet lifetime of 31 and 50 μs, respectively. Time dependent cellular uptake in MCF-7 cells for 1-Sn reached a peak at 24 h, and 1-Sn was found to be more lipophilic than 2-Sn. 1-Sn showed good photo-cytotoxicity on exposure to a Thorlabs 625 nm LED with an IC50 value of 3.2 μM and remained inactive in the dark.
- Full Text:
- Date Issued: 2019
Reciprocal peer reviews : evaluating potential bias and conflict of interest
- Authors: Baca, Zolile
- Date: 2022-12
- Subjects: Reciprocal , Peer review , Conflict of interests
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59358 , vital:62074
- Description: External quality assessments are conducted to provide independent reasonable assurance that an internal audit activity is operating in conformance with the Institute of Internal Auditors’ (IIA) Standards, Code of Ethics, and the extent of compliance with international best practices. The assurance provided by an internal audit activity is relied on by various stakeholders in the organisation for decision-making purposes. Therefore, it is crucial for an independent external quality assessment process to be credible and trustworthy for internal audit stakeholders to know that the internal audit activity conforms with the Standards and Code of Ethics, which in turn allows the stakeholders to rely on assurances and advice provided by the internal audit activity. The objective of this study was to determine the potential existence of a conflict of interest and or bias, in fact or in appearance, when reciprocal peer reviews are performed by people working within the same district and who are familiar with each other. A qualitative research method was followed to achieve the objective of this study and answer the primary research question. A single case study approach was followed in this study using municipalities in the Overberg District to answer the primary research question, with a purposive sampling method used to select relevant participants for the study. Participants were the Audit Committee members, the Municipal Managers, and the Chief Audit Executives from the municipalities in the Overberg District. An interview guide was administered by the researcher to collect the primary research data through semi-structured interviews. The interviews were electronically recorded after permission was obtained from each participant, and each interview session was also transcribed. The primary data collected was then analysed using the In vivo coding method. Each interview transcript was manually analysed to identify key words, phrases or statements that agreed or disagreed with the IIA’s guidance pronouncements in relation to the performance of external quality assessments, with a specific focus on the use of reciprocal peer reviews. The findings of this study indicated that most participants did not perceive reciprocal peer reviews performed by persons working within the same district to be without bias and or conflict of interest, in fact or in appearance because of familiarities and close relations that exist between persons working in the same district in the same sector. The findings revealed that participants would consider the process and results of reciprocal peer reviews to be credible and trustworthy if they were conducted across the district or even across the provincial borders. The findings further showed that participants perceived a full external assessment to be the most reliable and trustworthy method to be used because of the external assessors or assessment teams having the necessary training, knowledge, and experience to perform external quality assessments which give the credibility and trustworthiness that is required for the external quality assessment process.. , Thesis (MCOM) -- Faculty of Faculty of Business and Economics Science, School of Applied Accounting, 2022
- Full Text:
- Date Issued: 2022-12
- Authors: Baca, Zolile
- Date: 2022-12
- Subjects: Reciprocal , Peer review , Conflict of interests
- Language: English
- Type: Master's theses , Thesis
- Identifier: http://hdl.handle.net/10948/59358 , vital:62074
- Description: External quality assessments are conducted to provide independent reasonable assurance that an internal audit activity is operating in conformance with the Institute of Internal Auditors’ (IIA) Standards, Code of Ethics, and the extent of compliance with international best practices. The assurance provided by an internal audit activity is relied on by various stakeholders in the organisation for decision-making purposes. Therefore, it is crucial for an independent external quality assessment process to be credible and trustworthy for internal audit stakeholders to know that the internal audit activity conforms with the Standards and Code of Ethics, which in turn allows the stakeholders to rely on assurances and advice provided by the internal audit activity. The objective of this study was to determine the potential existence of a conflict of interest and or bias, in fact or in appearance, when reciprocal peer reviews are performed by people working within the same district and who are familiar with each other. A qualitative research method was followed to achieve the objective of this study and answer the primary research question. A single case study approach was followed in this study using municipalities in the Overberg District to answer the primary research question, with a purposive sampling method used to select relevant participants for the study. Participants were the Audit Committee members, the Municipal Managers, and the Chief Audit Executives from the municipalities in the Overberg District. An interview guide was administered by the researcher to collect the primary research data through semi-structured interviews. The interviews were electronically recorded after permission was obtained from each participant, and each interview session was also transcribed. The primary data collected was then analysed using the In vivo coding method. Each interview transcript was manually analysed to identify key words, phrases or statements that agreed or disagreed with the IIA’s guidance pronouncements in relation to the performance of external quality assessments, with a specific focus on the use of reciprocal peer reviews. The findings of this study indicated that most participants did not perceive reciprocal peer reviews performed by persons working within the same district to be without bias and or conflict of interest, in fact or in appearance because of familiarities and close relations that exist between persons working in the same district in the same sector. The findings revealed that participants would consider the process and results of reciprocal peer reviews to be credible and trustworthy if they were conducted across the district or even across the provincial borders. The findings further showed that participants perceived a full external assessment to be the most reliable and trustworthy method to be used because of the external assessors or assessment teams having the necessary training, knowledge, and experience to perform external quality assessments which give the credibility and trustworthiness that is required for the external quality assessment process.. , Thesis (MCOM) -- Faculty of Faculty of Business and Economics Science, School of Applied Accounting, 2022
- Full Text:
- Date Issued: 2022-12
A comprehensive approach to scalability assessment of ICTD projects : a case study of ICT4RED
- Authors: Baduza, Gugulethu Qhawekazi
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178435 , vital:42939
- Description: Access restricted until April 2023. , Thesis (PhD) -- Faculty of Commerce, Information Systems, 2021
- Full Text:
- Date Issued: 2021-04
- Authors: Baduza, Gugulethu Qhawekazi
- Date: 2021-04
- Subjects: To be added
- Language: English
- Type: thesis , text , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/178435 , vital:42939
- Description: Access restricted until April 2023. , Thesis (PhD) -- Faculty of Commerce, Information Systems, 2021
- Full Text:
- Date Issued: 2021-04
Design, Development and Testing of a Low-cost Sub-Joule μPPT for a PocketQube
- Authors: Bae, Ji Eun
- Date: 2022-04
- Subjects: Electric current converters , Pulse circuits , Plasma jets
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/58295 , vital:58953
- Description: Small satellites are unmanned spacecraft with small size and mass weighing less than 500kg. A small satellite called the CubeSat was created by two university professors to help students understand satellite design. The idea of small satellites caught on and they became popular due to their low cost, quick development time and easy deployment. The inexpensive nature of small satellites has helped lower the entry barrier to space and led to a movement called the “democratisation of space”. The popularity of small satellites has also caught the eye of private companies that recognise the potential of commercialising small satellite technologies. Nowadays, small satellites are being considered for more complex and challenging space missions. However for a small satellite to reach its full potential, it needs to be equipped with a proper propulsion system. Governments, space agencies, companies and universities around the world have started to research new innovative miniaturised space propulsion technologies. Nowadays, there are many newly developed miniaturised propulsion technologies available. The new propulsion systems are either sold by the companies and universities at a very high price, or research and development is closely guarded due to the potential commercial value of the propulsion system. Companies and universities have primarily focused on researching and developing top-of-the-line micro-propulsion devices to win lucrative research funds. This has resulted in a lack of research into cheap reliable micropropulsion as there have been no incentives for companies and universities to develop this area. As a result, fund-limited students and individuals have been left behind, defeating the purpose of small satellites. This dissertation focuses on designing and developing a low-cost sub-joule micro-PPT propulsion system for a PocketQube satellite. The first section covers the literature review, which looks at the different space propulsion technologies currently available. The next section covers the micro-PPT propulsion system’s mechanical and electrical design and development process. After the development process, the performance of the prototype is tested using various input parameters, as well as in vacuum conditions and over its lifetime. The test results show that the optimal performance is obtained with an input voltage supply of 5V at a pulse frequency of 0.5Hz, which achieves a minimal impulse bit v of 0.698μNs and thrust range of 0.349~1.071μN. In comparison to the STRaND-1 3U CubeSat’s PPT, performance data show that the developed μPPT propulsion system is a competitive propulsion solution, as it achieves more thrust with similar minimal impulse bit, using only one third of the power consumption. The μPPT propulsion system is able to produce 1980 shots so far, which is far lower relatively than other established PPTs due to the limitations resulting from capacitor failure. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Bae, Ji Eun
- Date: 2022-04
- Subjects: Electric current converters , Pulse circuits , Plasma jets
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/58295 , vital:58953
- Description: Small satellites are unmanned spacecraft with small size and mass weighing less than 500kg. A small satellite called the CubeSat was created by two university professors to help students understand satellite design. The idea of small satellites caught on and they became popular due to their low cost, quick development time and easy deployment. The inexpensive nature of small satellites has helped lower the entry barrier to space and led to a movement called the “democratisation of space”. The popularity of small satellites has also caught the eye of private companies that recognise the potential of commercialising small satellite technologies. Nowadays, small satellites are being considered for more complex and challenging space missions. However for a small satellite to reach its full potential, it needs to be equipped with a proper propulsion system. Governments, space agencies, companies and universities around the world have started to research new innovative miniaturised space propulsion technologies. Nowadays, there are many newly developed miniaturised propulsion technologies available. The new propulsion systems are either sold by the companies and universities at a very high price, or research and development is closely guarded due to the potential commercial value of the propulsion system. Companies and universities have primarily focused on researching and developing top-of-the-line micro-propulsion devices to win lucrative research funds. This has resulted in a lack of research into cheap reliable micropropulsion as there have been no incentives for companies and universities to develop this area. As a result, fund-limited students and individuals have been left behind, defeating the purpose of small satellites. This dissertation focuses on designing and developing a low-cost sub-joule micro-PPT propulsion system for a PocketQube satellite. The first section covers the literature review, which looks at the different space propulsion technologies currently available. The next section covers the micro-PPT propulsion system’s mechanical and electrical design and development process. After the development process, the performance of the prototype is tested using various input parameters, as well as in vacuum conditions and over its lifetime. The test results show that the optimal performance is obtained with an input voltage supply of 5V at a pulse frequency of 0.5Hz, which achieves a minimal impulse bit v of 0.698μNs and thrust range of 0.349~1.071μN. In comparison to the STRaND-1 3U CubeSat’s PPT, performance data show that the developed μPPT propulsion system is a competitive propulsion solution, as it achieves more thrust with similar minimal impulse bit, using only one third of the power consumption. The μPPT propulsion system is able to produce 1980 shots so far, which is far lower relatively than other established PPTs due to the limitations resulting from capacitor failure. , Thesis (MA) -- Faculty of Engineering, the Built Environment, and Technology, 2022
- Full Text:
- Date Issued: 2022-04
Callenges facing school management teams in the general education and training band schools
- Authors: Bafundi,Zealous Mapisa
- Date: 2011
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3538 , vital:43625
- Description: Abstract text The study investigated the factors that lead to the high failure rate of grade 12 learners of the two high schools in the Ngqeleni area of Libode Mega District of the Eastern Cape, South Africa. The study was qualitative in nature; therefore interviews were used as the method of collecting data. Seven SMT members, four parents and four learners were, randomly selected from the two schools in the Ngqeleni area of Libode Mega District. The researcher randomly selected the participants then interviewed them. The researcher used a video recoder as an instrument to record the responses from the participants. To analyse data, the researcher transcribed the interview responses and interpreted them. The main findings of the study showed that the high failure rate was caused by the following factors: over-croweded classes, shortage of teaching and learnin~ materials, lack of parental involvement, teachers' low morale, poor learner discipline, teachers experiencing problems in implementing government policies, work-load and as such teaching becoming stressful. The researcher in his study therefore recommends that the Department of Education plans more workshops for schools to capacitate them. There should be provision for more classrooms and incentives for teachers. Key Words: factors, high failure rate, grade 12, Ngqeleni area, Department of Education. , Thesis (MMED) -- Faculty of Educational Sciences, 2011
- Full Text:
- Date Issued: 2011
- Authors: Bafundi,Zealous Mapisa
- Date: 2011
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3538 , vital:43625
- Description: Abstract text The study investigated the factors that lead to the high failure rate of grade 12 learners of the two high schools in the Ngqeleni area of Libode Mega District of the Eastern Cape, South Africa. The study was qualitative in nature; therefore interviews were used as the method of collecting data. Seven SMT members, four parents and four learners were, randomly selected from the two schools in the Ngqeleni area of Libode Mega District. The researcher randomly selected the participants then interviewed them. The researcher used a video recoder as an instrument to record the responses from the participants. To analyse data, the researcher transcribed the interview responses and interpreted them. The main findings of the study showed that the high failure rate was caused by the following factors: over-croweded classes, shortage of teaching and learnin~ materials, lack of parental involvement, teachers' low morale, poor learner discipline, teachers experiencing problems in implementing government policies, work-load and as such teaching becoming stressful. The researcher in his study therefore recommends that the Department of Education plans more workshops for schools to capacitate them. There should be provision for more classrooms and incentives for teachers. Key Words: factors, high failure rate, grade 12, Ngqeleni area, Department of Education. , Thesis (MMED) -- Faculty of Educational Sciences, 2011
- Full Text:
- Date Issued: 2011
Formulation of methyldopa 250 mg tablets by direct compression using a quality by design approach
- Authors: Baguma, Murungi Isaac
- Date: 2022-04
- Subjects: Pharmaceutical chemistry , Tablets (Medicine)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/58575 , vital:59929
- Description: Various pharmaceutical materials are known to take up moisture during both storage and manufacture. This phenomenon, termed hygroscopy, can result in unacceptable levels of moisture, potentially having deleterious effects on product quality, and consequently product efficacy and safety. Drug inefficacy specifically can result in life-threatening complications for the pregnant mother with uncontrolled blood pressure. Marketed as immediate release tablets, methyldopa is an antihypertensive drug commonly used in South Africa to treat hypertensive disorders of pregnancy. Despite being in use for over 50 years, the physicochemical challenges posed by methyldopa remain ever present. Methyldopa is not only significantly hygroscopic, but also prone to oxidative and hydrolytic degradation that can be accelerated by moisture. Poor compression behaviour, another limitation of methyldopa, leaves the formulation scientist with a constellation of formulation hurdles that must be faced, understood and overcome. This is a task that can be tackled using elements of the Quality by Design (QbD) approach. Using this approach, the impact of these phenomena on tablet quality can be better understood and controlled. This study was aimed at using this approach to develop a formulation of methyldopa 250 mg tablets. Direct compression, a simple and moisture-free process, was selected for the manufacture of methyldopa tablets. After careful review of literature and consultation with experts, excipients were selected for the candidate formulation and preliminary concentrations established for each. Similarly, settings for mixing and compression variables were established. Adopted from the QbD framework, the risk posed to tablet quality by these factors was qualitatively analysed using Preliminary Hazard Analysis (PHA) and quantitatively evaluated using Failure Modes and Effect Analysis (FMEA). Eight (8) factors determined to be high risk were further investigated in a preliminary experiment that followed a 12-batch Plackett Burman design. Data from this experiment was used to draw statistical relationships between the aforementioned factors and tablet quality attributes, thus shedding light on excipients and process parameters with practically significant impact on methyldopa tablet quality. Multivariate Analysis of Variance (MANOVA) of the response data reported SSG concentration, citric acid monohydrate xxvii concentration and mixing speed as key factors (p<0.05), demonstrating the need to control these factors for quality targets to be met. These three factors underpinned the 16-batch definitive experiment that followed, conceived using a Box Behnken design (BBD) including four batches prepared at the centerpoint values in the region of experimentation. The optimal methyldopa tablet formulation was achieved at the following settings: 1.0 % m/m magnesium stearate, 1.0 % m/m colloidal silica, 3.9 % m/m sodium starch glycolate, 1.7 % m/m citric acid monohydrate, mixing speed of 101 rpm, 6 minutes of pre-lubrication mixing, 2 minutes of lubrication and compression speed of 20 rpm. These settings were included in the design space and subsequent control strategy. It was learned that, within the design space, the risk to tablet quality and, by extension, the patient, was insignificant. This demonstrated that, using the QbD approach, challenges to pharmaceutical development can be effectively overcome. , Thesis (MA) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Baguma, Murungi Isaac
- Date: 2022-04
- Subjects: Pharmaceutical chemistry , Tablets (Medicine)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10948/58575 , vital:59929
- Description: Various pharmaceutical materials are known to take up moisture during both storage and manufacture. This phenomenon, termed hygroscopy, can result in unacceptable levels of moisture, potentially having deleterious effects on product quality, and consequently product efficacy and safety. Drug inefficacy specifically can result in life-threatening complications for the pregnant mother with uncontrolled blood pressure. Marketed as immediate release tablets, methyldopa is an antihypertensive drug commonly used in South Africa to treat hypertensive disorders of pregnancy. Despite being in use for over 50 years, the physicochemical challenges posed by methyldopa remain ever present. Methyldopa is not only significantly hygroscopic, but also prone to oxidative and hydrolytic degradation that can be accelerated by moisture. Poor compression behaviour, another limitation of methyldopa, leaves the formulation scientist with a constellation of formulation hurdles that must be faced, understood and overcome. This is a task that can be tackled using elements of the Quality by Design (QbD) approach. Using this approach, the impact of these phenomena on tablet quality can be better understood and controlled. This study was aimed at using this approach to develop a formulation of methyldopa 250 mg tablets. Direct compression, a simple and moisture-free process, was selected for the manufacture of methyldopa tablets. After careful review of literature and consultation with experts, excipients were selected for the candidate formulation and preliminary concentrations established for each. Similarly, settings for mixing and compression variables were established. Adopted from the QbD framework, the risk posed to tablet quality by these factors was qualitatively analysed using Preliminary Hazard Analysis (PHA) and quantitatively evaluated using Failure Modes and Effect Analysis (FMEA). Eight (8) factors determined to be high risk were further investigated in a preliminary experiment that followed a 12-batch Plackett Burman design. Data from this experiment was used to draw statistical relationships between the aforementioned factors and tablet quality attributes, thus shedding light on excipients and process parameters with practically significant impact on methyldopa tablet quality. Multivariate Analysis of Variance (MANOVA) of the response data reported SSG concentration, citric acid monohydrate xxvii concentration and mixing speed as key factors (p<0.05), demonstrating the need to control these factors for quality targets to be met. These three factors underpinned the 16-batch definitive experiment that followed, conceived using a Box Behnken design (BBD) including four batches prepared at the centerpoint values in the region of experimentation. The optimal methyldopa tablet formulation was achieved at the following settings: 1.0 % m/m magnesium stearate, 1.0 % m/m colloidal silica, 3.9 % m/m sodium starch glycolate, 1.7 % m/m citric acid monohydrate, mixing speed of 101 rpm, 6 minutes of pre-lubrication mixing, 2 minutes of lubrication and compression speed of 20 rpm. These settings were included in the design space and subsequent control strategy. It was learned that, within the design space, the risk to tablet quality and, by extension, the patient, was insignificant. This demonstrated that, using the QbD approach, challenges to pharmaceutical development can be effectively overcome. , Thesis (MA) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
The link between behavioural plasticity and aerobic scope 1 phenotypes in predicting the survival of Chrysoblephus laticeps 2 under climate variability
- Authors: Bailey, Lauren Ashleigh
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422606 , vital:71961 , DOI 10.21504/10962/422606
- Description: Thermal variability in the marine environment is likely to have a considerable effect on fishes as it impacts physiological performance and vital (i.e metabolism, foraging and swimming style) and non-vital (i.e. reproductive fitness) energetic processes. When fish are subjected to thermal stress, they may primarily respond by changing their behaviour. Species that have broad phenotypic behavioural plasticity (i.e. defined as the ability to adjust behavioural activity in presiding environmental conditions in order to remain within their optimal thermal range) may have a competitive advantage. Fish behavioural plasticity may take many forms. Some species may seek out thermal refugia by changing their phenology or distribution, while others alter the timing of their seasonal and spawning migrations in response to a changing environment. Although fishes can use behavioural changes to cope with climate change impacts, there does appear to be variability in the behavioural responses within species. However, if alterations in behaviour are insufficient to ensure that the individual remains within their optimal thermal range, physiological acclimation (i.e. defined as the process in which an organism adjusts to prevailing conditions by broadening their thermal performance curve so that their performance is maximized in the new thermal environment) may be required. Therefore, there is a critical link between the behaviour and thermal physiology of fishes, particularly in a world where they are facing increasing thermal stress. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Bailey, Lauren Ashleigh
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422606 , vital:71961 , DOI 10.21504/10962/422606
- Description: Thermal variability in the marine environment is likely to have a considerable effect on fishes as it impacts physiological performance and vital (i.e metabolism, foraging and swimming style) and non-vital (i.e. reproductive fitness) energetic processes. When fish are subjected to thermal stress, they may primarily respond by changing their behaviour. Species that have broad phenotypic behavioural plasticity (i.e. defined as the ability to adjust behavioural activity in presiding environmental conditions in order to remain within their optimal thermal range) may have a competitive advantage. Fish behavioural plasticity may take many forms. Some species may seek out thermal refugia by changing their phenology or distribution, while others alter the timing of their seasonal and spawning migrations in response to a changing environment. Although fishes can use behavioural changes to cope with climate change impacts, there does appear to be variability in the behavioural responses within species. However, if alterations in behaviour are insufficient to ensure that the individual remains within their optimal thermal range, physiological acclimation (i.e. defined as the process in which an organism adjusts to prevailing conditions by broadening their thermal performance curve so that their performance is maximized in the new thermal environment) may be required. Therefore, there is a critical link between the behaviour and thermal physiology of fishes, particularly in a world where they are facing increasing thermal stress. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Sedimentology, reservoir properties and hydrocarbon potential of the southern Bredasdorp Basin, offshore of the Western Cape Province, South Africa
- Authors: Baiyegunhi, Temitope Love
- Date: 2021-05
- Subjects: Sediments (Geology) , Hydrocarbon reservoirs
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20753 , vital:46546
- Description: The Bredasdorp Basin has become the focus for exploration activity (i.e., seismic exploration and drilling) since the discovery of gas-condensate and oil reservoirs in the early 1980s. The basin has proven hydrocarbon reserves and potential for future discoveries. However, uncertainty about the sedimentological and petrographic characteristics, reservoir qualities, thermal maturity and hydrocarbon potential of the source/reservoir rocks has hindered further exploration, particularly in the southern part of the basin. To date, this part of the basin remains unexplored and partially understood with respect to petroleum systems evolution when compared to the central and northern parts of the basin. To fill the research gaps, exploration wells E-AH1, E-AJ1, E-BA1, E-BB1 and E-D3 were investigated so as to unravel the petrographic characteristics, depositional processes and paleoenvironment, tectonic provenance, paleoweathering, hydrocarbon potential, thermal maturity, diagenetic characterisitcs and reservoir qualities of the southern Bredasdorp Basin. The grain size textural parameters revealed that the southern Bredasdorp Basin sandstones are unimodal, predominantly fine-grained, moderately well sorted, mesokurtic and near-symmetrical. The bivariate plots of grain size textural parameters indicate that the depositional environments had been influenced mainly by river/beach/coastal dune conditions. The linear discriminate functions (LDF) diagrams show that the sediments are turbidity current deposits in a shallow marine environment. The Passega diagram revealed that the studied sandstones were mainly deposited by traction currents and beach process. In addition, the grain size log-probability curves and Passega diagram show the predominance of saltation and suspension modes of sediment transportation. Based on the inter-relationship of the various statistical parameters, it is deduced that the southern Bredasdorp Basin are mainly shallow marine deposits with signature of beach and coastal river processes. Based on the lithofacies analysis of the southern Bredasdorp Basin borehole cores, thirteen lithofacies were identified and grouped into six facies associations (FAs). The facies associations are: matrix supported conglomerate and massive sandstone (FA 1), ripple cross laminated, trough cross bedded and bioturbated sandstone (FA 2), massive sandstone with mudstone and shale interbeds (FA 3), alternating laminated to interbedded sandstone/siltstone and mudstone (FA 4), massive mudstone with minor interlamination of clay-rich sandstones and siltstone (FA 5), and carbonaceous laminated shale and mudstone with occasional siltstone laminae (FA 6). Facies associations FA1, FA2, FA3, FA4, FA5 and FA6 are interpreted as submarine channel-fills, submarine channel-levee, submarine sheet lobe, submarine lobe fring/overflow, basin plain deposits and deep sea floor/basin plain deposits, respectively. Sedimentological evidences from lithofacies interpretation revealed shallow marine environment as the main depositional environment, with minor contribution from the deep marine environment. Petrographic studies show that the southern Bredasdorp Basin sandstones chiefly consist of quartz (52.2–68.0percent), feldspar (10.0–18.0percent), and lithic fragments (5.0–10.2percent). The modal composition analysis revealed that the sandstones could be classified as subarkosic arenite and lithic arkose. The provenance ternary diagrams revealed that the rocks are mainly of continental block provenances (stable shields and basement uplifted areas) and complemented by recycled sands from an associated platform. The tectonic provenance studies show that the sandstones are typically rift-derived arenites and have undergone long-distance transport from the source area along the rift. In the regional context of the evolution of the Bredasdorp Basin, the results suggested that the basin developed on a passive rift setting (trailing edge) of the stable continental margins. The provenance discrimination diagrams based on major oxide geochemistry revealed that the sandstones are mainly of quartzose sedimentary provenance, while the mudrocks are of quartzose sedimentary and intermediate igneous provenances. The discrimination diagrams indicate that the southern Bredasdorp Basin sediments were mostly derived from a cratonic interior or recycled orogen. The bivariate plots of TiO2 versus Ni, TiO2 against Zr and La/Th versus Hf as well as the ternary diagrams of V–Ni–Th×10 suggest that the mudrocks and sandstones were derived from felsic igneous rocks. The tectonic setting discrimination diagrams support passive-active continental margin setting of the provenance. Chemical index of alteration (CIA) indices observed in the sandstones suggest that their source area underwent low to moderate degree of chemical weathering. However, the mudrocks have high CIA indices suggesting that the source area underwent more intense chemical weathering, possibly due to climatic and/or tectonic variations. The organic geochemistry results show that these rocks have total organic carbon, TOC contents ranging from 0.14 to 7.03 wt.percent. The hydrogen index (HI), oxygen index (OI), and hydrocarbon index (S2/S3) values vary between 24–263 mg HC/g TOC, 4–78 mg CO2/g TOC, and 0.01–18 mgHC/mgCO2 TOC, respectively, indicating predominantly Type III and IV kerogen with a minor amount of mixed Type II/III kerogen. The mean vitrinite reflectance values vary from 0.60–1.20percent, indicating that the samples are in the oil-generation window. The Tmax and PI values are consistent with the mean vitrinite reflectance values, indicating that the southern Bredasdorp Basin source rocks have entered the oil window and are considered as effective source rocks in the southern Bredasdorp Basin. The hydrocarbon genetic potential (SP), normalized oil content (NOC) and production index (PI) values all indicate poor to fair hydrocarbon generative potential. The main diagenetic processes that have affected the reservoir quality of the southern Bredasdorp Basin rocks are cementation by authigenic clay, carbonate and silica, growth of authigenic glauconite, dissolution of minerals and load compaction. These aforementioned diagenetic processes act differently in each borehole and at different depths. The influence of cementation and compaction is complex with no particular pattern with increasing depth, suggesting that diagenesis is the main challenge to reservoir characterization in the southern Bredasdorp Basin. The clays in the sandstones act as pore choking cement, which reduces porosity and permeability of the reservoir rocks. Reservoir quality of the sandstones has been improved to various extents due to the development of secondary porosity as a result of partial to complete dissolution of early calcite cement and some detrital grains (feldspars) and also affected by grain fracturing. The scattered plots of porosity and permeability versus cement+clays show good inverse correlations, suggesting that the reservoir quality is mainly controlled by cementation and authigenic clays. Based on the diagenetic study, it can be inferred that the potential reservoir quality of the southern Bredasdorp Basin sandstones is poor-moderate. , Thesis (PhD) -- Faculty of Science and Agriculture, 2021
- Full Text:
- Date Issued: 2021-05
- Authors: Baiyegunhi, Temitope Love
- Date: 2021-05
- Subjects: Sediments (Geology) , Hydrocarbon reservoirs
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20753 , vital:46546
- Description: The Bredasdorp Basin has become the focus for exploration activity (i.e., seismic exploration and drilling) since the discovery of gas-condensate and oil reservoirs in the early 1980s. The basin has proven hydrocarbon reserves and potential for future discoveries. However, uncertainty about the sedimentological and petrographic characteristics, reservoir qualities, thermal maturity and hydrocarbon potential of the source/reservoir rocks has hindered further exploration, particularly in the southern part of the basin. To date, this part of the basin remains unexplored and partially understood with respect to petroleum systems evolution when compared to the central and northern parts of the basin. To fill the research gaps, exploration wells E-AH1, E-AJ1, E-BA1, E-BB1 and E-D3 were investigated so as to unravel the petrographic characteristics, depositional processes and paleoenvironment, tectonic provenance, paleoweathering, hydrocarbon potential, thermal maturity, diagenetic characterisitcs and reservoir qualities of the southern Bredasdorp Basin. The grain size textural parameters revealed that the southern Bredasdorp Basin sandstones are unimodal, predominantly fine-grained, moderately well sorted, mesokurtic and near-symmetrical. The bivariate plots of grain size textural parameters indicate that the depositional environments had been influenced mainly by river/beach/coastal dune conditions. The linear discriminate functions (LDF) diagrams show that the sediments are turbidity current deposits in a shallow marine environment. The Passega diagram revealed that the studied sandstones were mainly deposited by traction currents and beach process. In addition, the grain size log-probability curves and Passega diagram show the predominance of saltation and suspension modes of sediment transportation. Based on the inter-relationship of the various statistical parameters, it is deduced that the southern Bredasdorp Basin are mainly shallow marine deposits with signature of beach and coastal river processes. Based on the lithofacies analysis of the southern Bredasdorp Basin borehole cores, thirteen lithofacies were identified and grouped into six facies associations (FAs). The facies associations are: matrix supported conglomerate and massive sandstone (FA 1), ripple cross laminated, trough cross bedded and bioturbated sandstone (FA 2), massive sandstone with mudstone and shale interbeds (FA 3), alternating laminated to interbedded sandstone/siltstone and mudstone (FA 4), massive mudstone with minor interlamination of clay-rich sandstones and siltstone (FA 5), and carbonaceous laminated shale and mudstone with occasional siltstone laminae (FA 6). Facies associations FA1, FA2, FA3, FA4, FA5 and FA6 are interpreted as submarine channel-fills, submarine channel-levee, submarine sheet lobe, submarine lobe fring/overflow, basin plain deposits and deep sea floor/basin plain deposits, respectively. Sedimentological evidences from lithofacies interpretation revealed shallow marine environment as the main depositional environment, with minor contribution from the deep marine environment. Petrographic studies show that the southern Bredasdorp Basin sandstones chiefly consist of quartz (52.2–68.0percent), feldspar (10.0–18.0percent), and lithic fragments (5.0–10.2percent). The modal composition analysis revealed that the sandstones could be classified as subarkosic arenite and lithic arkose. The provenance ternary diagrams revealed that the rocks are mainly of continental block provenances (stable shields and basement uplifted areas) and complemented by recycled sands from an associated platform. The tectonic provenance studies show that the sandstones are typically rift-derived arenites and have undergone long-distance transport from the source area along the rift. In the regional context of the evolution of the Bredasdorp Basin, the results suggested that the basin developed on a passive rift setting (trailing edge) of the stable continental margins. The provenance discrimination diagrams based on major oxide geochemistry revealed that the sandstones are mainly of quartzose sedimentary provenance, while the mudrocks are of quartzose sedimentary and intermediate igneous provenances. The discrimination diagrams indicate that the southern Bredasdorp Basin sediments were mostly derived from a cratonic interior or recycled orogen. The bivariate plots of TiO2 versus Ni, TiO2 against Zr and La/Th versus Hf as well as the ternary diagrams of V–Ni–Th×10 suggest that the mudrocks and sandstones were derived from felsic igneous rocks. The tectonic setting discrimination diagrams support passive-active continental margin setting of the provenance. Chemical index of alteration (CIA) indices observed in the sandstones suggest that their source area underwent low to moderate degree of chemical weathering. However, the mudrocks have high CIA indices suggesting that the source area underwent more intense chemical weathering, possibly due to climatic and/or tectonic variations. The organic geochemistry results show that these rocks have total organic carbon, TOC contents ranging from 0.14 to 7.03 wt.percent. The hydrogen index (HI), oxygen index (OI), and hydrocarbon index (S2/S3) values vary between 24–263 mg HC/g TOC, 4–78 mg CO2/g TOC, and 0.01–18 mgHC/mgCO2 TOC, respectively, indicating predominantly Type III and IV kerogen with a minor amount of mixed Type II/III kerogen. The mean vitrinite reflectance values vary from 0.60–1.20percent, indicating that the samples are in the oil-generation window. The Tmax and PI values are consistent with the mean vitrinite reflectance values, indicating that the southern Bredasdorp Basin source rocks have entered the oil window and are considered as effective source rocks in the southern Bredasdorp Basin. The hydrocarbon genetic potential (SP), normalized oil content (NOC) and production index (PI) values all indicate poor to fair hydrocarbon generative potential. The main diagenetic processes that have affected the reservoir quality of the southern Bredasdorp Basin rocks are cementation by authigenic clay, carbonate and silica, growth of authigenic glauconite, dissolution of minerals and load compaction. These aforementioned diagenetic processes act differently in each borehole and at different depths. The influence of cementation and compaction is complex with no particular pattern with increasing depth, suggesting that diagenesis is the main challenge to reservoir characterization in the southern Bredasdorp Basin. The clays in the sandstones act as pore choking cement, which reduces porosity and permeability of the reservoir rocks. Reservoir quality of the sandstones has been improved to various extents due to the development of secondary porosity as a result of partial to complete dissolution of early calcite cement and some detrital grains (feldspars) and also affected by grain fracturing. The scattered plots of porosity and permeability versus cement+clays show good inverse correlations, suggesting that the reservoir quality is mainly controlled by cementation and authigenic clays. Based on the diagenetic study, it can be inferred that the potential reservoir quality of the southern Bredasdorp Basin sandstones is poor-moderate. , Thesis (PhD) -- Faculty of Science and Agriculture, 2021
- Full Text:
- Date Issued: 2021-05
The optimisation of transportation methods for abalone (Haliotis midae Linnaeus, 1758 (Mollusca: Gastropoda)) larvae
- Authors: Bajaba, Sharone
- Date: 2021-02
- Subjects: Abalone fisheries , Haliotis midae , Abalone culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20561 , vital:46124
- Description: Sea ranching has been identified as a viable method for enhancing the natural stock of overexploited abalone (Haliotis midae). Currently, this process involves transporting live juvenile H. midae to the seeding site where they are released onto the reef however this is both costly and logistically problematic. Transportation of abalone larvae is another cost-effective option, as they are cheaper to produce and can be transported at high densities. A suitable larval transport method is required to minimise larval mortalities and stresses that might compromise settlement. A series of simulated experiments were conducted to optimise transportation systems of abalone (Haliotis midae) larvae. First, two potential transportation modes (Wet (W) and Dry (D) transportation) conducted at a cooler (14˚C) and average ambient (18˚C) temperatures with six replicates of each were compared with control treatments (six replicates) that were not subjected to transport, kept in water at 18˚C. Eighty hours post-initial settlement, the 14W treatment had significantly lower settlement (p=0.03) than the other three treatments (14D, 18W, 18D) and the Control. The Dry method was the prefered method to transport larvae as it is logistically simpler to employ. The second experiment investigated the effect of different stocking densities (200, 400 and 800 larvae cm-2) for the Dry method 18˚C over two transit periods (six and twelve hours) on post-transport settlement and post-settlement survival of H. midae larvae. Compared to the control, there was no difference in the number of settled larvae (p=0.368) and larvae still swimming (p=0.835) across all treatments. This suggested that H. midae larvae can be transported for twelve hours at 800 larvae cm-2 without compromising post-transport settlement or survival. This study’s results and recommendations can be used by abalone farms when there is a need to move abalone H. midae larvae between farms or to seeding sites for sea ranching. Furthermore, other researchers can use these results as a benchmark for larval transportation studies of H. midae and other abalone species. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Bajaba, Sharone
- Date: 2021-02
- Subjects: Abalone fisheries , Haliotis midae , Abalone culture
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20561 , vital:46124
- Description: Sea ranching has been identified as a viable method for enhancing the natural stock of overexploited abalone (Haliotis midae). Currently, this process involves transporting live juvenile H. midae to the seeding site where they are released onto the reef however this is both costly and logistically problematic. Transportation of abalone larvae is another cost-effective option, as they are cheaper to produce and can be transported at high densities. A suitable larval transport method is required to minimise larval mortalities and stresses that might compromise settlement. A series of simulated experiments were conducted to optimise transportation systems of abalone (Haliotis midae) larvae. First, two potential transportation modes (Wet (W) and Dry (D) transportation) conducted at a cooler (14˚C) and average ambient (18˚C) temperatures with six replicates of each were compared with control treatments (six replicates) that were not subjected to transport, kept in water at 18˚C. Eighty hours post-initial settlement, the 14W treatment had significantly lower settlement (p=0.03) than the other three treatments (14D, 18W, 18D) and the Control. The Dry method was the prefered method to transport larvae as it is logistically simpler to employ. The second experiment investigated the effect of different stocking densities (200, 400 and 800 larvae cm-2) for the Dry method 18˚C over two transit periods (six and twelve hours) on post-transport settlement and post-settlement survival of H. midae larvae. Compared to the control, there was no difference in the number of settled larvae (p=0.368) and larvae still swimming (p=0.835) across all treatments. This suggested that H. midae larvae can be transported for twelve hours at 800 larvae cm-2 without compromising post-transport settlement or survival. This study’s results and recommendations can be used by abalone farms when there is a need to move abalone H. midae larvae between farms or to seeding sites for sea ranching. Furthermore, other researchers can use these results as a benchmark for larval transportation studies of H. midae and other abalone species. , Thesis (MSc) (Zoology) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
Exploring senior phase teachers’ use of kahoot! Gamification as a formative assessment tool to scaffold mathematical understanding
- Authors: Balele, Silence
- Date: 2024-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/423891 , vital:72102
- Description: In South Africa, digital technology, particularly gamification, continues to become an important teaching and learning tool. Its importance lies in scaffolding mathematical understanding, improving the quality of assessment, and developing twenty-first-century technological skills for effective mathematics assessment. To explore this importance, senior phase mathematics teachers are encouraged to adopt gamification technology such as Kahoot! in assessment. The study adopted a case study research design with a interpretivist research paradigm, as this method allows detailed exploration and understanding of a specific context through a close connection with the participants. Data was collected using semi-structured questionnaires, focus group interviews, non-participant observations, and reflective journals. The research involved ten purposively selected senior-phase mathematics teachers from two schools in the Sarah Baartman District, Eastern Cape Province, South Africa. The study drew upon Vygoysky's Sociocultural Theory for its theoretical framework, while the Technological, Pedagogical, and Content Knowledge was used for analysis. The data analysis pursued an inductive approach. Adopting a thematic analysis included coding participant responses, organizing codes into concepts and categories, and developing a theoretical framework from the resultant categories. A four-phase process was involved: coding, conceptualizing, categorizing, and theorizing, was employed. This process and using NVivo software for data organization and management helped identify patterns, themes, and significant concerns surrounding the study's core subject. The findings from the study were as follows: Firstly, it was revealed that most teachers have a positive perception and attitude towards using Kahoot! for formative assessment, believing that it enhances teaching and learning quality. However, they showed scepticism due to insufficient Information and Communication Technologies knowledge and training. Second, teachers' pedagogical and technological experiences were predominantly enjoyable. Kahoot! was accepted as beneficial for promoting collaboration, interaction, and immediate feedback. Third, the study identified enabling and constraining factors in using Kahoot! for formative assessment. The enabling factors include Kahoot!, which was convenient and accessible, promoted immediate feedback and fostered collaboration and interaction. The constraining factors include Information and Communication Technologies and network interference, which posed limitations. The research concluded that Kahoot! as a platform for formative assessment indeed scaffold mathematical understanding. The study recommends increasing teachers' access to educational technology, incorporating continuous professional development programs, providing subject-specific training, and encouraging teachers to adapt to emerging technologies. The study also provided recommendations for future research to explore learner perceptions, employ a mixed methods approach to help quantify learner performance and investigate the pedagogical and technological experiences of senior phase mathematics teachers regarding formative assessment. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
- Full Text:
- Date Issued: 2024-04
- Authors: Balele, Silence
- Date: 2024-04
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/423891 , vital:72102
- Description: In South Africa, digital technology, particularly gamification, continues to become an important teaching and learning tool. Its importance lies in scaffolding mathematical understanding, improving the quality of assessment, and developing twenty-first-century technological skills for effective mathematics assessment. To explore this importance, senior phase mathematics teachers are encouraged to adopt gamification technology such as Kahoot! in assessment. The study adopted a case study research design with a interpretivist research paradigm, as this method allows detailed exploration and understanding of a specific context through a close connection with the participants. Data was collected using semi-structured questionnaires, focus group interviews, non-participant observations, and reflective journals. The research involved ten purposively selected senior-phase mathematics teachers from two schools in the Sarah Baartman District, Eastern Cape Province, South Africa. The study drew upon Vygoysky's Sociocultural Theory for its theoretical framework, while the Technological, Pedagogical, and Content Knowledge was used for analysis. The data analysis pursued an inductive approach. Adopting a thematic analysis included coding participant responses, organizing codes into concepts and categories, and developing a theoretical framework from the resultant categories. A four-phase process was involved: coding, conceptualizing, categorizing, and theorizing, was employed. This process and using NVivo software for data organization and management helped identify patterns, themes, and significant concerns surrounding the study's core subject. The findings from the study were as follows: Firstly, it was revealed that most teachers have a positive perception and attitude towards using Kahoot! for formative assessment, believing that it enhances teaching and learning quality. However, they showed scepticism due to insufficient Information and Communication Technologies knowledge and training. Second, teachers' pedagogical and technological experiences were predominantly enjoyable. Kahoot! was accepted as beneficial for promoting collaboration, interaction, and immediate feedback. Third, the study identified enabling and constraining factors in using Kahoot! for formative assessment. The enabling factors include Kahoot!, which was convenient and accessible, promoted immediate feedback and fostered collaboration and interaction. The constraining factors include Information and Communication Technologies and network interference, which posed limitations. The research concluded that Kahoot! as a platform for formative assessment indeed scaffold mathematical understanding. The study recommends increasing teachers' access to educational technology, incorporating continuous professional development programs, providing subject-specific training, and encouraging teachers to adapt to emerging technologies. The study also provided recommendations for future research to explore learner perceptions, employ a mixed methods approach to help quantify learner performance and investigate the pedagogical and technological experiences of senior phase mathematics teachers regarding formative assessment. , Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 2024
- Full Text:
- Date Issued: 2024-04
The effect of academic staff development on lecturer teaching practices at a historically disadvantaged institution: lecturer’ perspectives
- Baleni, Zwelijongile Gaylard
- Authors: Baleni, Zwelijongile Gaylard
- Date: 2021-00
- Subjects: Universities and colleges -- Professional staff
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/8021 , vital:59331
- Description: This study aimed to determine the lecturers’ perspective on the role of Academic Staff Development (ASD) in enhancing and upskilling lecturers' teaching practices and strategies to improve student learning. The study was undertaken at a comprehensive HE Institution in the Eastern Cape province of South Africa. This study was based on the critical theory which advances change, transformation, emancipation, contradiction, alienation and domination, tenets that academic staff development also advocates. Academic development is supported by principles upheld by critical theory, for example, empowerment, transformation and democracy. For this study, the population comprised those lecturers who attended academic staff development workshops as from 2015 to 2017 at the HDI. In line with the Quanti-Quali mixed explanatory sequential approach, I collected quantitative data from academics using questionnaires; followed by interviews to collect qualitative data. The results showed that the majority appreciated academic staff development as a career changing experience as it capacitated them to teach, grounding them in pedagogical content knowledge in addition to their discipline specialisations. Some constraining conditions to the uptake of ASD at the HDI emerged from the study. Due to the privileging of research over teaching, ASD was seen as a waste of their research time. Institutional environment constraints such as huge workloads, constraining departmental cultures; poor timing of ASD workshops, poor institutional communication about ASD activities and lack of monitoring of implementing those skills, were classified as hindrances. Finally, it is clear that lecturers are made, not born. Recommendations are that it must be compulsory for all academics to attend ASD workshops at least bi-annually. ASDs should be planned and conducted in three phases, namely, pre-workshop, workshop and post-workshop phases. , Thesis (Doctoral) -- Faculty of Education Sciences, 2021
- Full Text:
- Date Issued: 2021-00
- Authors: Baleni, Zwelijongile Gaylard
- Date: 2021-00
- Subjects: Universities and colleges -- Professional staff
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/11260/8021 , vital:59331
- Description: This study aimed to determine the lecturers’ perspective on the role of Academic Staff Development (ASD) in enhancing and upskilling lecturers' teaching practices and strategies to improve student learning. The study was undertaken at a comprehensive HE Institution in the Eastern Cape province of South Africa. This study was based on the critical theory which advances change, transformation, emancipation, contradiction, alienation and domination, tenets that academic staff development also advocates. Academic development is supported by principles upheld by critical theory, for example, empowerment, transformation and democracy. For this study, the population comprised those lecturers who attended academic staff development workshops as from 2015 to 2017 at the HDI. In line with the Quanti-Quali mixed explanatory sequential approach, I collected quantitative data from academics using questionnaires; followed by interviews to collect qualitative data. The results showed that the majority appreciated academic staff development as a career changing experience as it capacitated them to teach, grounding them in pedagogical content knowledge in addition to their discipline specialisations. Some constraining conditions to the uptake of ASD at the HDI emerged from the study. Due to the privileging of research over teaching, ASD was seen as a waste of their research time. Institutional environment constraints such as huge workloads, constraining departmental cultures; poor timing of ASD workshops, poor institutional communication about ASD activities and lack of monitoring of implementing those skills, were classified as hindrances. Finally, it is clear that lecturers are made, not born. Recommendations are that it must be compulsory for all academics to attend ASD workshops at least bi-annually. ASDs should be planned and conducted in three phases, namely, pre-workshop, workshop and post-workshop phases. , Thesis (Doctoral) -- Faculty of Education Sciences, 2021
- Full Text:
- Date Issued: 2021-00
Soil and vegetation recovery following Acacia dealbata clearing in the Tsitsa catchment, Eastern Cape Province of South Africa: implications for ecological restoration
- Authors: Balintulo, Putuma
- Date: 2022-04-06
- Subjects: Acacia South Africa Eastern Cape , Invasive plants South Africa Eastern Cape , Working for Water Programme , Soil restoration South Africa Eastern Cape , Plant nutrients South Africa Eastern Cape , Restoration ecology South Africa Eastern Cape , Clearing of land South Africa Eastern Cape , Legacy effect
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/290778 , vital:56783
- Description: Invasion by alien plant species in South Africa continues to compromise the stability of ecosystems by causing declines in biodiversity, altering soil nutrients and processes, and subsequently transforming ecosystem functionality. Control of invasive alien plant species has been widely implemented in South Africa to minimize their negative impacts; however, the legacy effects can persist long after the plant has been removed. The impacts of Acacia dealbata clearing on soil properties and native vegetation recovery remains understudied despite their significance in ecological restoration and monitoring. This comparative study determined the impacts of A. dealbata clearing on both soil physicochemical properties and vegetation in the Eastern Cape Province of South Africa. Soils were collected from three different clearing treatments, namely, cleared, invaded, and uninvaded, on 5 m x 5 m plots over three summer months. The plots were replicated four times for each clearing treatment, making a total of 72 sampling plots. Soils were assessed for soil pH, resistivity, P, C, N, and exchangeable cations as well as soil moisture content, penetration resistance, infiltration rate, hydraulic conductivity, and water repellency. Clearing of A. dealbata did not have any significant effects on most soil nutrients, however, there were variations in soil pH, resistance, and Na. Soil pH was significantly higher in the uninvaded treatments than in the cleared and invaded treatments. Soil moisture content was significantly higher in the cleared treatments than the adjacent invaded and uninvaded treatments, but this was observed in the month of December only. Soil penetration resistance and infiltration rates were significantly higher in the month of December in the cleared treatments. For all clearing treatments, no significant differences were recorded for soil hydraulic conductivity. These results on changes in soil properties following A. dealbata clearing are varied, with some soil properties showing decreases, an indication that removal of A. dealbata has the potential to shift soil properties towards a positive recovery trajectory. This study further assessed whether the clearing of A. dealbata facilitates the recovery of native plant species. Vegetation surveys were conducted in the three above-mentioned treatments and plots. Results showed little recruitment of native grasses and forbs, but the persistence of A. dealbata seedlings in the cleared treatments. Species richness and Shannon-Wiener diversity index were significantly (P < 0.05) higher in the cleared and invaded treatments than the uninvaded treatments, and this was more visible for trees and shrubs. Cover for all species was significantly higher (P < 0.05) in the uninvaded than the cleared and invaded treatments. This study observed the recruitment of some native species in the cleared treatments that were not present in the invaded treatments. Therefore, the recruitment and establishment of some native species, mostly grasses, in the cleared treatments gives assurance that passive restoration is on a positive vegetation recovery trajectory that can lead to recovery of native vegetation after A. dealbata clearing. Therefore, the study concludes that investing in ecological restoration after alien plant clearing is a necessity for complete ecosystem recovery to be achieved. Overall, the study concludes that the removal of A. dealbata triggers changes to some soil properties. Similarly, the study observed recruitment of some native grasses in cleared areas, an indication that alien plant clearing facilitates changes in both soil properties and vegetation. However, soil and vegetation recovery are being hampered by the regrowth of A. dealbata and secondary invaders that were observed in the cleared treatments. Two key recommendations of this study are (i) clearing follow-up to remove recruiting seedlings of invasive plant species and secondary invaders should be timeous and well-funded, and (ii) active restoration should be considered to speed-up soil and vegetation recovery processes. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2022
- Full Text:
- Date Issued: 2022-04-06
- Authors: Balintulo, Putuma
- Date: 2022-04-06
- Subjects: Acacia South Africa Eastern Cape , Invasive plants South Africa Eastern Cape , Working for Water Programme , Soil restoration South Africa Eastern Cape , Plant nutrients South Africa Eastern Cape , Restoration ecology South Africa Eastern Cape , Clearing of land South Africa Eastern Cape , Legacy effect
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/290778 , vital:56783
- Description: Invasion by alien plant species in South Africa continues to compromise the stability of ecosystems by causing declines in biodiversity, altering soil nutrients and processes, and subsequently transforming ecosystem functionality. Control of invasive alien plant species has been widely implemented in South Africa to minimize their negative impacts; however, the legacy effects can persist long after the plant has been removed. The impacts of Acacia dealbata clearing on soil properties and native vegetation recovery remains understudied despite their significance in ecological restoration and monitoring. This comparative study determined the impacts of A. dealbata clearing on both soil physicochemical properties and vegetation in the Eastern Cape Province of South Africa. Soils were collected from three different clearing treatments, namely, cleared, invaded, and uninvaded, on 5 m x 5 m plots over three summer months. The plots were replicated four times for each clearing treatment, making a total of 72 sampling plots. Soils were assessed for soil pH, resistivity, P, C, N, and exchangeable cations as well as soil moisture content, penetration resistance, infiltration rate, hydraulic conductivity, and water repellency. Clearing of A. dealbata did not have any significant effects on most soil nutrients, however, there were variations in soil pH, resistance, and Na. Soil pH was significantly higher in the uninvaded treatments than in the cleared and invaded treatments. Soil moisture content was significantly higher in the cleared treatments than the adjacent invaded and uninvaded treatments, but this was observed in the month of December only. Soil penetration resistance and infiltration rates were significantly higher in the month of December in the cleared treatments. For all clearing treatments, no significant differences were recorded for soil hydraulic conductivity. These results on changes in soil properties following A. dealbata clearing are varied, with some soil properties showing decreases, an indication that removal of A. dealbata has the potential to shift soil properties towards a positive recovery trajectory. This study further assessed whether the clearing of A. dealbata facilitates the recovery of native plant species. Vegetation surveys were conducted in the three above-mentioned treatments and plots. Results showed little recruitment of native grasses and forbs, but the persistence of A. dealbata seedlings in the cleared treatments. Species richness and Shannon-Wiener diversity index were significantly (P < 0.05) higher in the cleared and invaded treatments than the uninvaded treatments, and this was more visible for trees and shrubs. Cover for all species was significantly higher (P < 0.05) in the uninvaded than the cleared and invaded treatments. This study observed the recruitment of some native species in the cleared treatments that were not present in the invaded treatments. Therefore, the recruitment and establishment of some native species, mostly grasses, in the cleared treatments gives assurance that passive restoration is on a positive vegetation recovery trajectory that can lead to recovery of native vegetation after A. dealbata clearing. Therefore, the study concludes that investing in ecological restoration after alien plant clearing is a necessity for complete ecosystem recovery to be achieved. Overall, the study concludes that the removal of A. dealbata triggers changes to some soil properties. Similarly, the study observed recruitment of some native grasses in cleared areas, an indication that alien plant clearing facilitates changes in both soil properties and vegetation. However, soil and vegetation recovery are being hampered by the regrowth of A. dealbata and secondary invaders that were observed in the cleared treatments. Two key recommendations of this study are (i) clearing follow-up to remove recruiting seedlings of invasive plant species and secondary invaders should be timeous and well-funded, and (ii) active restoration should be considered to speed-up soil and vegetation recovery processes. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2022
- Full Text:
- Date Issued: 2022-04-06