The roots run deep?: Understanding branch politics within the African National Congress
- Authors: Mkhutle, Nhlanhla Handsome
- Date: 2021-10-29
- Subjects: African National Congress , Manipulative behavior Political aspects South Africa , Cartels South Africa , Power (Social sciences) South Africa , Patronage, Political South Africa , Political party organization South Africa , Branch politics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190261 , vital:44978
- Description: This thesis explores the functioning of branch politics within the African National Congress (ANC) and the influence of branch politics on the organization and its structures. The ANC has many of the characteristics of a mass party. In mass parties, mass participation is supposed to be promoted through the activities of party branches. This thesis documents the weakening of the ANC’s local level structures and their political control over the organization’s decisions. ANC branches are investigated as democratic vanguards of ANC’s mass party persona, in relation to their functioning as the most basic units of the organization. The thesis reflects on branch members’ experiences in different ANC branches to understand the interplay between branch politics, political power, and patronage politics within the ANC. This research project also explores the possibility of manipulation of ANC branches by senior leaders and would-be-leaders of the ANC. The thesis then concludes by summarising the state of branch politics within the ANC and giving recommendations on further study with regards to branch politics of the ANC. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Mkhutle, Nhlanhla Handsome
- Date: 2021-10-29
- Subjects: African National Congress , Manipulative behavior Political aspects South Africa , Cartels South Africa , Power (Social sciences) South Africa , Patronage, Political South Africa , Political party organization South Africa , Branch politics
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190261 , vital:44978
- Description: This thesis explores the functioning of branch politics within the African National Congress (ANC) and the influence of branch politics on the organization and its structures. The ANC has many of the characteristics of a mass party. In mass parties, mass participation is supposed to be promoted through the activities of party branches. This thesis documents the weakening of the ANC’s local level structures and their political control over the organization’s decisions. ANC branches are investigated as democratic vanguards of ANC’s mass party persona, in relation to their functioning as the most basic units of the organization. The thesis reflects on branch members’ experiences in different ANC branches to understand the interplay between branch politics, political power, and patronage politics within the ANC. This research project also explores the possibility of manipulation of ANC branches by senior leaders and would-be-leaders of the ANC. The thesis then concludes by summarising the state of branch politics within the ANC and giving recommendations on further study with regards to branch politics of the ANC. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Fabrication of gold-nanoparticle/conductive polymer composite materials: application to aptamer-based impedimetric biosensors for detection of histamine
- Authors: Ojo, Dupe Ruth
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192550 , vital:45236
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Ojo, Dupe Ruth
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192550 , vital:45236
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
Photosynthetic microbial fuel cells and fabricated photobioreactors applied with halotolerant microorganisms for nutrient and metal remediation under adverse hypersaline
- Authors: Smith, Galad Gilbert
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192561 , vital:45237
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Smith, Galad Gilbert
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192561 , vital:45237
- Description: Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
Genetic connectivity of the roundjaw bonefish (Albula glossodonta) in the Southwest Indian Ocean
- Talma, Sheena Claudia Aisa Lydie
- Authors: Talma, Sheena Claudia Aisa Lydie
- Date: 2021-10-29
- Subjects: Bonefish Mauritius , Bonefish Seychelles , Bonefish Genetics , Bonefish Habitat , Bonefish Geographical distribution , Bonefish Larvae Dispersal , Genetic markers , Cytochrome b , Fish populations Mauritius , Fish populations Seychelles , Marine ecotourism , Saltwater fly fishing , Bonefish fisheries Catch effort
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192174 , vital:45202
- Description: The Southwest Indian Ocean (SWIO) islands of Mauritius and Seychelles are both highly dependent on tourism and fisheries for their economies. One of the growing ecotourism sectors is saltwater fly fishing, an industry based on catch-and-release fishing for a host of species, including bonefishes. Bonefishes (Albula spp.) have received significant research attention in the Pacific and Atlantic Oceans, with only sporadic research conducted in the Indian Ocean. My project aimed to investigate the genetic connectivity of the roundjaw bonefish (Albula glossodonta) in two island states (Seychelles and Mauritius) within the SWIO using a mitochondrial genetic marker (cyt-b) and next generation sequencing (ddRADseq). Samples collected were grouped based on their spatial distribution. The Seychelles consisted of four island groups (Inner Island Group, Aldabra Group, Amirantes and Alphonse Group, and Farquhar Group) whereas Mauritius was represented by one island group (Saint Brandon). Genetic analyses were undertaken between and within each of these groups. Mitochondrial cytochrome-b identified two species of bonefish: Albula glossodonta and Albula oligolepis; the latter was only genetically identified from the Inner Island Group. I hypothesise that this is due to habitat partitioning, with A. oligolepis being a deeper dwelling bonefish species compared to A. glossodonta, which occupies shallow water habitats such as sand flats, atoll lagoons and reef flats. Neutral SNP loci revealed a panmictic pattern of distribution for A. glossodonta throughout the Seychelles Island groups but showed a pattern of weak structure between Seychelles and Mauritius. Genetic diversity indices such as allelic richness, showed low diversity across the sampling sites (AR range: 1.761-1.889). Population structure tests such as pairwise FST showed low but significant population structure. The highest FST indices were recorded between the Aldabra and Farquhar Groups, as well as the Aldabra and Saint Brandon Groups (0.044 ± 0.000 and 0.040 ± 0.000, respectively). Descriptive tests such as PCA and DAPC showed similar trends, whereby Saint Brandon clustered separately from the other samples from the Seychelles Island groups. However, these trends were Abstract seen at very low variations (PCA axes 1 and 2 accounted for only 2.0 and 1.9 % of the total variation, respectively). A population assignment test grouped the individuals as one ancestral population. A spatial principal component analysis showed that Saint Brandon was dissimilar to the Seychelles Island groups. Like other Elopomorph species, bonefishes have leptocephalus larvae capable of long-distance dispersal which may explain the well-mixed genetic population observed within the Seychelles islands. Although currents within the Indian Ocean, especially on a mesoscale, are not well understood, the South Equatorial Current likely facilitates connectivity between the Seychelles islands while also limiting gene flow between Seychelles and Mauritius. Understanding population structure is important for informing the appropriate management and conservation strategies, especially in oceanic nations where data informing important industries like tourism and fisheries are often limited. The bonefish fly fishing industry is well-known to be a lucrative sector, generating, for example US$ 1.4 million a year in the Bahamas. This study recognised that there are numerous knowledge gaps relevant to the bonefish industry that need to be addressed, including: 1) understanding the socio-economic importance of fly fishing to island states like Seychelles, 2) estimating the abundance and species distribution of bonefishes within Seychelles, 3) understanding effectiveness of MPAs for recreational fishery species like bonefish and, lastly, 4) generating more fishery-relevant biological information on the heavily targeted fly fishing species within Seychelles. These needs must be met to inform management plans and to better manage the fly fishing ventures that target species like bonefish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Talma, Sheena Claudia Aisa Lydie
- Date: 2021-10-29
- Subjects: Bonefish Mauritius , Bonefish Seychelles , Bonefish Genetics , Bonefish Habitat , Bonefish Geographical distribution , Bonefish Larvae Dispersal , Genetic markers , Cytochrome b , Fish populations Mauritius , Fish populations Seychelles , Marine ecotourism , Saltwater fly fishing , Bonefish fisheries Catch effort
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192174 , vital:45202
- Description: The Southwest Indian Ocean (SWIO) islands of Mauritius and Seychelles are both highly dependent on tourism and fisheries for their economies. One of the growing ecotourism sectors is saltwater fly fishing, an industry based on catch-and-release fishing for a host of species, including bonefishes. Bonefishes (Albula spp.) have received significant research attention in the Pacific and Atlantic Oceans, with only sporadic research conducted in the Indian Ocean. My project aimed to investigate the genetic connectivity of the roundjaw bonefish (Albula glossodonta) in two island states (Seychelles and Mauritius) within the SWIO using a mitochondrial genetic marker (cyt-b) and next generation sequencing (ddRADseq). Samples collected were grouped based on their spatial distribution. The Seychelles consisted of four island groups (Inner Island Group, Aldabra Group, Amirantes and Alphonse Group, and Farquhar Group) whereas Mauritius was represented by one island group (Saint Brandon). Genetic analyses were undertaken between and within each of these groups. Mitochondrial cytochrome-b identified two species of bonefish: Albula glossodonta and Albula oligolepis; the latter was only genetically identified from the Inner Island Group. I hypothesise that this is due to habitat partitioning, with A. oligolepis being a deeper dwelling bonefish species compared to A. glossodonta, which occupies shallow water habitats such as sand flats, atoll lagoons and reef flats. Neutral SNP loci revealed a panmictic pattern of distribution for A. glossodonta throughout the Seychelles Island groups but showed a pattern of weak structure between Seychelles and Mauritius. Genetic diversity indices such as allelic richness, showed low diversity across the sampling sites (AR range: 1.761-1.889). Population structure tests such as pairwise FST showed low but significant population structure. The highest FST indices were recorded between the Aldabra and Farquhar Groups, as well as the Aldabra and Saint Brandon Groups (0.044 ± 0.000 and 0.040 ± 0.000, respectively). Descriptive tests such as PCA and DAPC showed similar trends, whereby Saint Brandon clustered separately from the other samples from the Seychelles Island groups. However, these trends were Abstract seen at very low variations (PCA axes 1 and 2 accounted for only 2.0 and 1.9 % of the total variation, respectively). A population assignment test grouped the individuals as one ancestral population. A spatial principal component analysis showed that Saint Brandon was dissimilar to the Seychelles Island groups. Like other Elopomorph species, bonefishes have leptocephalus larvae capable of long-distance dispersal which may explain the well-mixed genetic population observed within the Seychelles islands. Although currents within the Indian Ocean, especially on a mesoscale, are not well understood, the South Equatorial Current likely facilitates connectivity between the Seychelles islands while also limiting gene flow between Seychelles and Mauritius. Understanding population structure is important for informing the appropriate management and conservation strategies, especially in oceanic nations where data informing important industries like tourism and fisheries are often limited. The bonefish fly fishing industry is well-known to be a lucrative sector, generating, for example US$ 1.4 million a year in the Bahamas. This study recognised that there are numerous knowledge gaps relevant to the bonefish industry that need to be addressed, including: 1) understanding the socio-economic importance of fly fishing to island states like Seychelles, 2) estimating the abundance and species distribution of bonefishes within Seychelles, 3) understanding effectiveness of MPAs for recreational fishery species like bonefish and, lastly, 4) generating more fishery-relevant biological information on the heavily targeted fly fishing species within Seychelles. These needs must be met to inform management plans and to better manage the fly fishing ventures that target species like bonefish. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2021
- Full Text:
- Date Issued: 2021-10-29
An evolutionary study of legless skinks’ (Acontias Cuvier, 1817) head and vertebrae morphology
- Authors: Evlambiou, Anthony Andreas
- Date: 2021-10
- Subjects: Skinks South Africa , Acontias South Africa , Typhlosaurus South Africa , Acontias Morphology , Acontias Phylogeny , Acontias Evolution , Vertebrae , Skull Growth , Evolutionary developmental biology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190690 , vital:45018
- Description: Environmental factors and/or processes can produce differences in general shape between individuals or particular parts of individuals. Examples of these biological processes may include ontogenetic development, adaptation to local geographic factors, or long-term evolutionary diversification. An organism is not likely to be able to optimise a single structure for multiple purposes and so trade-offs are likely to occur. An example of such a structure is the cranium, as it can be used for multiple activities such as defensive and sexual behaviour, locomotion, prey capture, and ingestion. Morphological characteristics have historically been used in the description of species. Genetic analyses have gained popularity as species delineation techniques and have been particularly useful in identifying cryptic species, especially among morphological conserved species like legless skinks of the subfamily Acontinae (e.g. Acontias Cuvier, 1817 and Typhlosaurus Weigmann, 1834). However, completely doing away with morphological techniques during species descriptions is not the best option. Therefore, novel methods to identify species, especially those with similar body plans, are needed. In this dissertation, we explore the links between head shape and vertebral number to environmental pressures to determine whether the evolutionary process is driven by environmental pressures (soil or biome) or is retained through ancestry. A novel species/clade delineation linked to vertebral number is also investigated. Head shape was expected to have a close link to the environment and the number of vertebrae was expected to have a closer link to ancestry. The first chapter investigates the drivers behind Acontias head shape evolution using geometric morphometric techniques. We found that environmental pressures did affect the evolution of head shape especially in the “soil” and “biome” categories but further investigation is advised. The second chapter explores the viability of using vertebral counts as a novel method for species and/or clade delineation in Acontias and to determine whether vertebral number can be linked to the environment. Delineating species based on vertebral count is likely not an option, however, delineating clades proved to show promising results. A link between vertebral count and environment was found in Acontias with larger bodied species occurring in different environments to smaller body species. In conclusion, the genus Acontias is difficult to delineate morphologically. Genetic sequence analyses can indicate differences and delineate the species. Even though there were differences in morphology based on environmental factors, it is not sufficient to delineate this subfamily alone. Further research is advised and this dissertation provides a good basis to work with. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Evlambiou, Anthony Andreas
- Date: 2021-10
- Subjects: Skinks South Africa , Acontias South Africa , Typhlosaurus South Africa , Acontias Morphology , Acontias Phylogeny , Acontias Evolution , Vertebrae , Skull Growth , Evolutionary developmental biology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190690 , vital:45018
- Description: Environmental factors and/or processes can produce differences in general shape between individuals or particular parts of individuals. Examples of these biological processes may include ontogenetic development, adaptation to local geographic factors, or long-term evolutionary diversification. An organism is not likely to be able to optimise a single structure for multiple purposes and so trade-offs are likely to occur. An example of such a structure is the cranium, as it can be used for multiple activities such as defensive and sexual behaviour, locomotion, prey capture, and ingestion. Morphological characteristics have historically been used in the description of species. Genetic analyses have gained popularity as species delineation techniques and have been particularly useful in identifying cryptic species, especially among morphological conserved species like legless skinks of the subfamily Acontinae (e.g. Acontias Cuvier, 1817 and Typhlosaurus Weigmann, 1834). However, completely doing away with morphological techniques during species descriptions is not the best option. Therefore, novel methods to identify species, especially those with similar body plans, are needed. In this dissertation, we explore the links between head shape and vertebral number to environmental pressures to determine whether the evolutionary process is driven by environmental pressures (soil or biome) or is retained through ancestry. A novel species/clade delineation linked to vertebral number is also investigated. Head shape was expected to have a close link to the environment and the number of vertebrae was expected to have a closer link to ancestry. The first chapter investigates the drivers behind Acontias head shape evolution using geometric morphometric techniques. We found that environmental pressures did affect the evolution of head shape especially in the “soil” and “biome” categories but further investigation is advised. The second chapter explores the viability of using vertebral counts as a novel method for species and/or clade delineation in Acontias and to determine whether vertebral number can be linked to the environment. Delineating species based on vertebral count is likely not an option, however, delineating clades proved to show promising results. A link between vertebral count and environment was found in Acontias with larger bodied species occurring in different environments to smaller body species. In conclusion, the genus Acontias is difficult to delineate morphologically. Genetic sequence analyses can indicate differences and delineate the species. Even though there were differences in morphology based on environmental factors, it is not sufficient to delineate this subfamily alone. Further research is advised and this dissertation provides a good basis to work with. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
The prominence of choral music in the search for, and preservation of, an African identity: A study focusing on the role of choral composers in the formation of black nationalism during and after the colonial era in South Africa
- Authors: Nelani, Athenkosi
- Date: 2021-10-29
- Subjects: Choral music South Africa , Choral singing South Africa , Black people Race identity South Africa , Black nationalism South Africa , Composers, Black South Africa , Amakwaya
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190965 , vital:45045
- Description: In South Africa, choral music has been prominent in schools, churches, and community choirs since the 19th century. However, the majority of South Africans know little of the history of indigenous choral music. This study investigates the origins of South African indigenous choral music, its canonic figures, and their contribution to this genre in promoting an African identity among the indigenous people of South Africa. Using the methodological lens of historical ethnomusicology, this thesis explores the genesis of choral music in South Africa. Choral music was introduced to the region when it was first colonised by the Dutch East India Company in 1652. Missionaries established institutions in the villages of the local people and translated the Bible and hymn books into the local languages. These actions had lasting consequences for music, and choral singing was greatly advanced. In addition, in this work early composers of South African indigenous choral music are recognised in an effort to establish a choral canon. Three definitive choral periods are recognised, namely those of the first-generation, second-generation, and post-colonial composers. Using this historical framework, this thesis investigates the social and political influence these composers had on African identity during the liberation struggles in the late colonial era and during the early apartheid period. The history and compositions of the composers are discussed, including how they used their compositions as political tools and as mouthpieces to communicate societal issues of concern to the indigenous people during and after the colonial era. , Thesis (MMus) -- Faculty of Humanities, Music and Musicology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Nelani, Athenkosi
- Date: 2021-10-29
- Subjects: Choral music South Africa , Choral singing South Africa , Black people Race identity South Africa , Black nationalism South Africa , Composers, Black South Africa , Amakwaya
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190965 , vital:45045
- Description: In South Africa, choral music has been prominent in schools, churches, and community choirs since the 19th century. However, the majority of South Africans know little of the history of indigenous choral music. This study investigates the origins of South African indigenous choral music, its canonic figures, and their contribution to this genre in promoting an African identity among the indigenous people of South Africa. Using the methodological lens of historical ethnomusicology, this thesis explores the genesis of choral music in South Africa. Choral music was introduced to the region when it was first colonised by the Dutch East India Company in 1652. Missionaries established institutions in the villages of the local people and translated the Bible and hymn books into the local languages. These actions had lasting consequences for music, and choral singing was greatly advanced. In addition, in this work early composers of South African indigenous choral music are recognised in an effort to establish a choral canon. Three definitive choral periods are recognised, namely those of the first-generation, second-generation, and post-colonial composers. Using this historical framework, this thesis investigates the social and political influence these composers had on African identity during the liberation struggles in the late colonial era and during the early apartheid period. The history and compositions of the composers are discussed, including how they used their compositions as political tools and as mouthpieces to communicate societal issues of concern to the indigenous people during and after the colonial era. , Thesis (MMus) -- Faculty of Humanities, Music and Musicology, 2021
- Full Text:
- Date Issued: 2021-10-29
An analysis of citizen participation in service delivery in Zimbabwe’s small towns: A case study of Chiredzi Town Council
- Authors: Matsilele, Blessing
- Date: 2021-10-29
- Subjects: Municipal services Zimbabwe Chiredzi Rural District , Political participation Zimbabwe Chiredzi Rural District , Social capital (Sociology) Zimbabwe Chiredzi Rural District , Municipal services Zimbabwe Chiredzi Rural District Citizen participation , Community development Zimbabwe Chiredzi Rural District
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190099 , vital:44963
- Description: Over the years, there have been complaints of poor service delivery in Zimbabwe’s Urban Councils. Newspapers in Zimbabwe have been filled with stories of poor service delivery and citizen grievances. Existing literature on service delivery has focused on the causes of poor service delivery, blaming them solely on the local government authorities. The literature has blamed poor service delivery in Zimbabwe on corruption, polarisation, politicisation, and incapacitation amongst other things. What is not highlighted is the role that citizens play and their responsibility in ensuring that there is proper service delivery. This thesis discusses the ways in which citizens participate in local government service delivery in Chiredzi which is an urban Council in Zimbabwe. To establish citizen participation in Chiredzi, this theory draws upon the social capital theory focusing on its trust, network, and norms aspect. Social capital facilitates an analysis of citizen participation and service delivery in Chiredzi. For this study,a qualitative research methodology was used. This methodology consisted of semi-structured telephone interviews with participants together with relevant documents. It was concluded that there is a need to educate citizens on their roles and responsibilities in local government to improve the state of service delivery. , Thesis (MSocSci) -- Faculty of Humanities, Sociology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Matsilele, Blessing
- Date: 2021-10-29
- Subjects: Municipal services Zimbabwe Chiredzi Rural District , Political participation Zimbabwe Chiredzi Rural District , Social capital (Sociology) Zimbabwe Chiredzi Rural District , Municipal services Zimbabwe Chiredzi Rural District Citizen participation , Community development Zimbabwe Chiredzi Rural District
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190099 , vital:44963
- Description: Over the years, there have been complaints of poor service delivery in Zimbabwe’s Urban Councils. Newspapers in Zimbabwe have been filled with stories of poor service delivery and citizen grievances. Existing literature on service delivery has focused on the causes of poor service delivery, blaming them solely on the local government authorities. The literature has blamed poor service delivery in Zimbabwe on corruption, polarisation, politicisation, and incapacitation amongst other things. What is not highlighted is the role that citizens play and their responsibility in ensuring that there is proper service delivery. This thesis discusses the ways in which citizens participate in local government service delivery in Chiredzi which is an urban Council in Zimbabwe. To establish citizen participation in Chiredzi, this theory draws upon the social capital theory focusing on its trust, network, and norms aspect. Social capital facilitates an analysis of citizen participation and service delivery in Chiredzi. For this study,a qualitative research methodology was used. This methodology consisted of semi-structured telephone interviews with participants together with relevant documents. It was concluded that there is a need to educate citizens on their roles and responsibilities in local government to improve the state of service delivery. , Thesis (MSocSci) -- Faculty of Humanities, Sociology, 2021
- Full Text:
- Date Issued: 2021-10-29
A self-emulsifying delivery system loaded with efavirenz: The case for flax-seed oil
- Authors: Mazonde, Priveledge
- Date: 2021-10-29
- Subjects: Drug delivery systems , Linseed oil , Antiretroviral agents , HIV (Viruses) , Drug carriers (Pharmacy) , Solubility , High performance liquid chromatography , Efavirenz
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192944 , vital:45283
- Description: The feasibility of incorporating efavirenz (EFV), an antiretroviral agent against HIV into a lipid-based self-emulsifying drug delivery system (SEDDS) containing vegetable oils was investigated. EFV has poor aqueous solubility and is classified under the Biopharmaceutical Classification System (BCS) as a class II compound with highly permeability, its aqueous solubility is less than 10 mg/ml and is defined as a practically insoluble compound with a consequent poor bioavailability of approximately 40%, and erratic dissolution behaviour. SEDDS formulations have been shown to improve the aqueous solubility and consequently the bioavailability of BCS II compounds such as EFV. EFV is a first line antiviral agent used in combination with other agents in antiretroviral therapy (ART). Among the number of NNRTIs approved for use in HIV treatment, EFV is one of the most commonly prescribed drug. Statistical methods and Design of Experiments (DoE) using Response Surface Methodology (RSM), specifically a Central Composite Design (CCD), were used to facilitate the development of a reversed-phase high performance liquid chromatographic (HPLC) method for the quantitation of EFV during formulation product and process development studies. A rapid, accurate, precise and sensitive HPLC method with ultraviolet (UV) detection was developed, optimised and validated for the in-vitro analysis of EFV in a total run time under 10 minutes for the elution of both EFV and loratidine which was used as the internal standard (IS). The method was then successfully applied to the determination of EFV in commercially available tablets. Excipient screening was undertaken using solubility studies and revealed that EFV had highest solubility in flaxseed oil in comparison to soybean, macadamia, grapeseed, sunflower and olive oils. The non-ionic Tween® 80 and Span® 20 were selected as surfactant and co-surfactant, respectively with ethanol co-solvent as they exhibited improved miscibility with co-solvent. Pre-formulation studies were undertaken to investigate the compatibility of the API with excipients and to identify a nano-emulsion region and other emulsion types using pseudoternary phase diagrams. The phase behaviour of crude cold pressed flaxseed oil with the selected non-ionic surfactants revealed an area within pseudo-ternary phase diagrams for different surfactant-mixtures formed gels/semisolid structures which can be exploited for other drug delivery strategies that require such properties. Fourier transform infrared spectroscopy (FT-IR), powder x-ray diffraction (XRD) and Raman spectroscopy were used to identify and assess the compatibility of EFV with chosen excipients. 2 A reduction in the peak intensity was observed for EFV when combined with each hydrophobic/lipid excipient evaluated revealing that there was a marked reduction in the crystallinity of the EFV. A decrease in crystallinity in comparison with the bulk API may indicate that EFV were amorphous or sequestered in a molecular dispersion and exhibited an increased solubility for the molecule. Flaxseed oil was used as the oil phase in studies for the optimization of surfactant mixtures undertaken using DoE, specifically a D-optimal mixtures design with the flaxseed oil content set at 10% m/m was performed. Solutions from the desired optimization function were produced based on desirability and five nanoemulsion formulations were produced and characterized in terms of in vitro release of efavirenz, drug loading capacity, Zeta Potential, droplet sizes and polydispersity index (PDI). Kinetically stable nanoemulsions containing 10% m/m flaxseed oil were successfully manufactured and assessed. Droplet sizes ranged between 156 and 225 nm, Zeta Potential between −24 and −41 mV and all formulations were found to be monodisperse with polydispersity indices ≤ 0.487. SEDDS formulations of EFV in nano-sized carriers were developed and optimised, in vitro drug release varied with varying amounts of ethanol in the formulation producing formulations that exhibited differently modulated drug in-vitro release profiles that may be further manipulated for better performance and therapeutic outcomes in terms of solubility and possibly bioavailability of EFV when delivered using SEDDS rather than using tablets which in turn may lead to better therapeutic outcomes for patients with HIV. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Mazonde, Priveledge
- Date: 2021-10-29
- Subjects: Drug delivery systems , Linseed oil , Antiretroviral agents , HIV (Viruses) , Drug carriers (Pharmacy) , Solubility , High performance liquid chromatography , Efavirenz
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192944 , vital:45283
- Description: The feasibility of incorporating efavirenz (EFV), an antiretroviral agent against HIV into a lipid-based self-emulsifying drug delivery system (SEDDS) containing vegetable oils was investigated. EFV has poor aqueous solubility and is classified under the Biopharmaceutical Classification System (BCS) as a class II compound with highly permeability, its aqueous solubility is less than 10 mg/ml and is defined as a practically insoluble compound with a consequent poor bioavailability of approximately 40%, and erratic dissolution behaviour. SEDDS formulations have been shown to improve the aqueous solubility and consequently the bioavailability of BCS II compounds such as EFV. EFV is a first line antiviral agent used in combination with other agents in antiretroviral therapy (ART). Among the number of NNRTIs approved for use in HIV treatment, EFV is one of the most commonly prescribed drug. Statistical methods and Design of Experiments (DoE) using Response Surface Methodology (RSM), specifically a Central Composite Design (CCD), were used to facilitate the development of a reversed-phase high performance liquid chromatographic (HPLC) method for the quantitation of EFV during formulation product and process development studies. A rapid, accurate, precise and sensitive HPLC method with ultraviolet (UV) detection was developed, optimised and validated for the in-vitro analysis of EFV in a total run time under 10 minutes for the elution of both EFV and loratidine which was used as the internal standard (IS). The method was then successfully applied to the determination of EFV in commercially available tablets. Excipient screening was undertaken using solubility studies and revealed that EFV had highest solubility in flaxseed oil in comparison to soybean, macadamia, grapeseed, sunflower and olive oils. The non-ionic Tween® 80 and Span® 20 were selected as surfactant and co-surfactant, respectively with ethanol co-solvent as they exhibited improved miscibility with co-solvent. Pre-formulation studies were undertaken to investigate the compatibility of the API with excipients and to identify a nano-emulsion region and other emulsion types using pseudoternary phase diagrams. The phase behaviour of crude cold pressed flaxseed oil with the selected non-ionic surfactants revealed an area within pseudo-ternary phase diagrams for different surfactant-mixtures formed gels/semisolid structures which can be exploited for other drug delivery strategies that require such properties. Fourier transform infrared spectroscopy (FT-IR), powder x-ray diffraction (XRD) and Raman spectroscopy were used to identify and assess the compatibility of EFV with chosen excipients. 2 A reduction in the peak intensity was observed for EFV when combined with each hydrophobic/lipid excipient evaluated revealing that there was a marked reduction in the crystallinity of the EFV. A decrease in crystallinity in comparison with the bulk API may indicate that EFV were amorphous or sequestered in a molecular dispersion and exhibited an increased solubility for the molecule. Flaxseed oil was used as the oil phase in studies for the optimization of surfactant mixtures undertaken using DoE, specifically a D-optimal mixtures design with the flaxseed oil content set at 10% m/m was performed. Solutions from the desired optimization function were produced based on desirability and five nanoemulsion formulations were produced and characterized in terms of in vitro release of efavirenz, drug loading capacity, Zeta Potential, droplet sizes and polydispersity index (PDI). Kinetically stable nanoemulsions containing 10% m/m flaxseed oil were successfully manufactured and assessed. Droplet sizes ranged between 156 and 225 nm, Zeta Potential between −24 and −41 mV and all formulations were found to be monodisperse with polydispersity indices ≤ 0.487. SEDDS formulations of EFV in nano-sized carriers were developed and optimised, in vitro drug release varied with varying amounts of ethanol in the formulation producing formulations that exhibited differently modulated drug in-vitro release profiles that may be further manipulated for better performance and therapeutic outcomes in terms of solubility and possibly bioavailability of EFV when delivered using SEDDS rather than using tablets which in turn may lead to better therapeutic outcomes for patients with HIV. , Thesis (MSc) -- Faculty of Pharmacy, Pharmacy, 2021
- Full Text:
- Date Issued: 2021-10-29
Cellphones and whistles: Exploring the communicative ecology and sociality of the Enkanini informal settlement in Makhanda
- Authors: Baloyi, Karabo
- Date: 2021-10-29
- Subjects: Communication models , Cell phones Social aspects South Africa Makhanda , Cell phones Economic aspects South Africa Makhanda , Squatter settlements South Africa Makhanda , South Africa Social conditions 1994- , South Africa Economic conditions 1991- , South Africa Social life and customs , Communication Economic aspects South Africa Makhanda , Whistles South Africa Makhanda , Decolonization South Africa Makhanda , Communicative ecology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191046 , vital:45053
- Description: This thesis explores the communicative ecology in the Enkanini informal settlement in Makhanda, and in particular their use of mobile phones and whistles to build a sense of community. It makes the case for word-of-mouth as an integral part of the communicative ecology despite not being a technological device. It then examines the sociality that arises from the use of these devices, and how coloniality impacts on the participants’ everyday experiences. The research was conducted through telephonic in-depth interviews with participants. To corroborate some of the content drawn from interviews, I used Grocott’s Mail, Makhanda’s only independent newspaper as an archival source, as well as some of the participants’ Facebook profiles. This research argues that as a consequence of coloniality, Enkanini’s residents suffer socio-economic challenges, and thus are unable to use digital technologies as much as they might like to, to communicate with their neighbours. As a consequence, word-of-mouth is their main form of communication with one another. Whistles are the ‘low-tech’ device used for community-wide communication to alert residents of an emergency, or about a meeting or protest. My findings contest generalised claims of society moving towards a network sociality, where individuality and project-based communication is valued over more communal forms of living. They also demonstrate the ways in which coloniality shapes almost every aspect of marginalised people’s lives, making word of mouth the most significant form of communication, notwithstanding the apparent availability of digital technology. It also shows how a marginalised group uses the resources it has to pressure local government officials to provide them with the basis infrastructure they need for survival. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Baloyi, Karabo
- Date: 2021-10-29
- Subjects: Communication models , Cell phones Social aspects South Africa Makhanda , Cell phones Economic aspects South Africa Makhanda , Squatter settlements South Africa Makhanda , South Africa Social conditions 1994- , South Africa Economic conditions 1991- , South Africa Social life and customs , Communication Economic aspects South Africa Makhanda , Whistles South Africa Makhanda , Decolonization South Africa Makhanda , Communicative ecology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191046 , vital:45053
- Description: This thesis explores the communicative ecology in the Enkanini informal settlement in Makhanda, and in particular their use of mobile phones and whistles to build a sense of community. It makes the case for word-of-mouth as an integral part of the communicative ecology despite not being a technological device. It then examines the sociality that arises from the use of these devices, and how coloniality impacts on the participants’ everyday experiences. The research was conducted through telephonic in-depth interviews with participants. To corroborate some of the content drawn from interviews, I used Grocott’s Mail, Makhanda’s only independent newspaper as an archival source, as well as some of the participants’ Facebook profiles. This research argues that as a consequence of coloniality, Enkanini’s residents suffer socio-economic challenges, and thus are unable to use digital technologies as much as they might like to, to communicate with their neighbours. As a consequence, word-of-mouth is their main form of communication with one another. Whistles are the ‘low-tech’ device used for community-wide communication to alert residents of an emergency, or about a meeting or protest. My findings contest generalised claims of society moving towards a network sociality, where individuality and project-based communication is valued over more communal forms of living. They also demonstrate the ways in which coloniality shapes almost every aspect of marginalised people’s lives, making word of mouth the most significant form of communication, notwithstanding the apparent availability of digital technology. It also shows how a marginalised group uses the resources it has to pressure local government officials to provide them with the basis infrastructure they need for survival. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2021
- Full Text:
- Date Issued: 2021-10-29
Modification of Gelatin-Methacrylate, Hyaluronic-Methacrylate and Poly(ethylene) glycol Diacrylate hydrogel bioinks towards the additive manufacturing of articular cartilage
- Authors: Barwick, Matthew William
- Date: 2021-10
- Subjects: Cartilage Diseases , Cartilage Regeneration , Articular cartilage Diseases , Chondrogenesis , Stem cells , Scanning electron microscopy , Fourier transform infrared spectroscopy , Three-dimensional printing , Gelatin-Methacrylate , Hyaluronic-Methacrylate , Poly(ethylene) glycolDiacrylate , Hydrogel bioinks , Real-Time Quantitative Cell Analysis (RTCA) , Bioprinting
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191181 , vital:45068
- Description: Cartilage degradation is most commonly associated with Rheumatoid arthritis and Osteoarthritis, affecting millions of people worldwide. Joint transplants commonly use titanium alloys, which have a shelf life of between 10-15 years. Although the titanium transplant restores partial mobility, side effects such as inflammation, swelling, faulty implants, and metal poisoning in some cases resulting from the transplant. The use of additive manufacturing of articular cartilage sheds new, innovative prospects for joint replacements. This study sets out to formulate and characterize five different hydrogel types towards the additive manufacturing of articular cartilage. Chondrogenic and Adipogenic differentiation was carried out on two separate adipose-mesenchymal stem cell lines A270620-01A, and A311019-02T and validation and efficiency of the differentiation and chondrogenic gene expression was carried out using Alcian Blue stain, Oil Red O stain and Quantitative Reverse Transcription PCR (RT-qPCR). Hydrogel formulation and characterisation of 10 % Gelatin-methacryloyl (GelMA), 10 % Poly (ethylene) glycol diacrylate (PEGDA), 5 % GelMA/5 % PEGDA, 10 % GelMA/0.5 % Hyaluronic Acid Methacrylate (HAMA) and 10 % PEGDA/0.5 % HAMA was carried out through swelling and degradation ratios, surface area and porosity characterisation using Scanning Electron Microscopy (SEM). Hydrogel component and spectroscopic analysis were carried using Real-Time Quantitative Cell Analysis (RTCA) and Fourier-transform Infrared Spectroscopy (FTIR) analysis for each formulated hydrogel's chemical characterisation. Three-dimensional printing (3D) of 10 % PEGDA/0.5 % HAMA and 5 % GelMA/5 % PEGDA was performed using the Zortrax INKSPIRE Resin Ultra-Violet (UV) LCD Desktop 3D Printer. Hydrogel sterility and cell viability were carried out for each hydrogel type using fluorescence microscopy. Both A270620-01A and A311019-02T cell lines showed adipogenic and chondrogenic differentiation ability, with A311019-02T cell line showing greater chondrogenic differentiation of Alcian blue staining. The A270620-01A cell line resulted in a greater collagen gene expression based on the RT-qPCR results. The hydrogel 10 % GelMA showed the greatest swelling ratio of 1260 % in DPBS and 1192 % in DMEM. A significant difference between hydrogel swelling and swelling with Dulbecco's Phosphate Buffered Saline (DPBS) and Dulbecco’s Modified Eagle Medium (DMEM) was observed. The 10 % PEGDA hydrogel had the greatest degradation ratio of 59 % mass remaining, where the 10 % GelMA/0.5 % HAMA showed the least amount of degradation with a mass remaining at 91 %. The 10 % GelMA showed the greatest porosity will the largest pore size of 14 μm in diameter. Hydrogel component and spectroscopic analysis showed no cytotoxic effects for the visible light photoinitiator used to polymerize the hydrogel and no cytotoxic effects for the concentrations used in chondrogenic differentiation. The FTIR analysis showed partial gelatin and hyaluronic acid modification with methacrylic anhydride; however, the distinction between the hybrid hydrogels and single polymer hydrogels could not be made effectively. UV and ethanol washing showed to completely sterilise the hydrogel disks from any contaminants, making them suitable for tissue culture. The cell viability analysis showed the 10 % GelMA/HAMA having the highest cell viability of 77.3 % using 5000 cells/disk and 89.64 % viability using 50 000 cells/disk over a 7-day incubation period. Overall, the combination of two polymers, GelMA and HAMA, has good potential as a 3D hydrogel scaffold towards additive manufacturing of articular cartilage. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Barwick, Matthew William
- Date: 2021-10
- Subjects: Cartilage Diseases , Cartilage Regeneration , Articular cartilage Diseases , Chondrogenesis , Stem cells , Scanning electron microscopy , Fourier transform infrared spectroscopy , Three-dimensional printing , Gelatin-Methacrylate , Hyaluronic-Methacrylate , Poly(ethylene) glycolDiacrylate , Hydrogel bioinks , Real-Time Quantitative Cell Analysis (RTCA) , Bioprinting
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191181 , vital:45068
- Description: Cartilage degradation is most commonly associated with Rheumatoid arthritis and Osteoarthritis, affecting millions of people worldwide. Joint transplants commonly use titanium alloys, which have a shelf life of between 10-15 years. Although the titanium transplant restores partial mobility, side effects such as inflammation, swelling, faulty implants, and metal poisoning in some cases resulting from the transplant. The use of additive manufacturing of articular cartilage sheds new, innovative prospects for joint replacements. This study sets out to formulate and characterize five different hydrogel types towards the additive manufacturing of articular cartilage. Chondrogenic and Adipogenic differentiation was carried out on two separate adipose-mesenchymal stem cell lines A270620-01A, and A311019-02T and validation and efficiency of the differentiation and chondrogenic gene expression was carried out using Alcian Blue stain, Oil Red O stain and Quantitative Reverse Transcription PCR (RT-qPCR). Hydrogel formulation and characterisation of 10 % Gelatin-methacryloyl (GelMA), 10 % Poly (ethylene) glycol diacrylate (PEGDA), 5 % GelMA/5 % PEGDA, 10 % GelMA/0.5 % Hyaluronic Acid Methacrylate (HAMA) and 10 % PEGDA/0.5 % HAMA was carried out through swelling and degradation ratios, surface area and porosity characterisation using Scanning Electron Microscopy (SEM). Hydrogel component and spectroscopic analysis were carried using Real-Time Quantitative Cell Analysis (RTCA) and Fourier-transform Infrared Spectroscopy (FTIR) analysis for each formulated hydrogel's chemical characterisation. Three-dimensional printing (3D) of 10 % PEGDA/0.5 % HAMA and 5 % GelMA/5 % PEGDA was performed using the Zortrax INKSPIRE Resin Ultra-Violet (UV) LCD Desktop 3D Printer. Hydrogel sterility and cell viability were carried out for each hydrogel type using fluorescence microscopy. Both A270620-01A and A311019-02T cell lines showed adipogenic and chondrogenic differentiation ability, with A311019-02T cell line showing greater chondrogenic differentiation of Alcian blue staining. The A270620-01A cell line resulted in a greater collagen gene expression based on the RT-qPCR results. The hydrogel 10 % GelMA showed the greatest swelling ratio of 1260 % in DPBS and 1192 % in DMEM. A significant difference between hydrogel swelling and swelling with Dulbecco's Phosphate Buffered Saline (DPBS) and Dulbecco’s Modified Eagle Medium (DMEM) was observed. The 10 % PEGDA hydrogel had the greatest degradation ratio of 59 % mass remaining, where the 10 % GelMA/0.5 % HAMA showed the least amount of degradation with a mass remaining at 91 %. The 10 % GelMA showed the greatest porosity will the largest pore size of 14 μm in diameter. Hydrogel component and spectroscopic analysis showed no cytotoxic effects for the visible light photoinitiator used to polymerize the hydrogel and no cytotoxic effects for the concentrations used in chondrogenic differentiation. The FTIR analysis showed partial gelatin and hyaluronic acid modification with methacrylic anhydride; however, the distinction between the hybrid hydrogels and single polymer hydrogels could not be made effectively. UV and ethanol washing showed to completely sterilise the hydrogel disks from any contaminants, making them suitable for tissue culture. The cell viability analysis showed the 10 % GelMA/HAMA having the highest cell viability of 77.3 % using 5000 cells/disk and 89.64 % viability using 50 000 cells/disk over a 7-day incubation period. Overall, the combination of two polymers, GelMA and HAMA, has good potential as a 3D hydrogel scaffold towards additive manufacturing of articular cartilage. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10
The tuber-feeding weevil Listronotus frontalis as a candidate biological control agent for the invasive semi-aquatic plant Sagittaria platyphylla within South Africa
- Authors: Rogers, Daniel James
- Date: 2021-10
- Subjects: Listronotus South Africa , Arrowhead (Plants) South Africa , Arrowhead (Plants) Biological control South Africa , Invasive plants Biological control South Africa , Insects as biological pest control agents South Africa , Plant populations South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190731 , vital:45023
- Description: Sagittaria platyphylla (Engelm.) J.G.Sm. (Alismataceae) is an invasive, aquatic macrophyte originating in the southern United States of America. In South Africa, the plant was first detected in Krantzkloof Nature Reserve, KwaZulu-Natal Province in 2008, and due to its known impact in other countries, it was listed as a Category 1a invader species under the National Environmental Management: Biodiversity Act 2004 (NEM:BA). This invasive plant has proved difficult to manage due to its varied growth forms and reproductive strategies, such as prolific seed and below ground tuber production. Due to the limitations of conventional control mechanisms, biological control is currently being considered as a potential control option. The tuber feeding weevil Listronotus frontalis LeConte (Coleoptera: Curculionidae) has been identified as a candidate biological control agent for this invasive species. The aims of this study were twofold; to firstly determine the importance of tubers to S. platyphylla populations growing in South Africa; and secondly, to determine the biology and suitability of L. frontalis, a tuber feeder, as a candidate biological control agent. Surveys of S. platyphylla populations in South Africa showed that tubers were found in all sampled sites, except for Krantzkloof Nature reserve in KwaZulu-Natal Province. The highest number of tubers was 97.75 ± 10.62 (SE) m-2 recorded at Jonkershoek in the Western Cape Province. Monthly sampling from two sites in the Eastern Cape Province, the Makana Botanical Gardens and Maden Dam showed that neither season nor water depth affected tuber production. However, the mean number of tubers as well as mass of tubers sampled, were consistently higher (F(1,179) = 20.9542, P < 0.0001) and heavier (F(1, 857) = 585.7293, P < 0.0001) at the Botanical Gardens than at Maden Dam, respectively. The study showed that tubers are an important life stage of S. platyphylla populations and may vary in size and abundance between and within sites. The tuber feeding weevil was shown to develop from egg to ovipositing adult within just over 40 days. Females were recorded to lay up to 48 eggs within a period of one week. Impact studies showed that adult feeding led to a reduction in all but one of the 11 measured plant growth and developmental measurements, including a reduction in the mean mass of the above-ground plant material (F(2,2743) = 12.05, P = 0.002) as well as a reduction in size and abundance of tubers (F(2,58.47) = 9.756, P = 0.0006) and stolons(F(14.943) = 8.7577, P = 0.003). These results are encouraging and suggest that if the insect is released in South Africa, it may prove to be a valuable biocontrol agent. It is concluded that, until suitable biological control options become available in South Africa, the chemical and mechanical control measures currently implemented should continue, however, controlling tubers should be considered during the planning and implementation of these strategies. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Rogers, Daniel James
- Date: 2021-10
- Subjects: Listronotus South Africa , Arrowhead (Plants) South Africa , Arrowhead (Plants) Biological control South Africa , Invasive plants Biological control South Africa , Insects as biological pest control agents South Africa , Plant populations South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190731 , vital:45023
- Description: Sagittaria platyphylla (Engelm.) J.G.Sm. (Alismataceae) is an invasive, aquatic macrophyte originating in the southern United States of America. In South Africa, the plant was first detected in Krantzkloof Nature Reserve, KwaZulu-Natal Province in 2008, and due to its known impact in other countries, it was listed as a Category 1a invader species under the National Environmental Management: Biodiversity Act 2004 (NEM:BA). This invasive plant has proved difficult to manage due to its varied growth forms and reproductive strategies, such as prolific seed and below ground tuber production. Due to the limitations of conventional control mechanisms, biological control is currently being considered as a potential control option. The tuber feeding weevil Listronotus frontalis LeConte (Coleoptera: Curculionidae) has been identified as a candidate biological control agent for this invasive species. The aims of this study were twofold; to firstly determine the importance of tubers to S. platyphylla populations growing in South Africa; and secondly, to determine the biology and suitability of L. frontalis, a tuber feeder, as a candidate biological control agent. Surveys of S. platyphylla populations in South Africa showed that tubers were found in all sampled sites, except for Krantzkloof Nature reserve in KwaZulu-Natal Province. The highest number of tubers was 97.75 ± 10.62 (SE) m-2 recorded at Jonkershoek in the Western Cape Province. Monthly sampling from two sites in the Eastern Cape Province, the Makana Botanical Gardens and Maden Dam showed that neither season nor water depth affected tuber production. However, the mean number of tubers as well as mass of tubers sampled, were consistently higher (F(1,179) = 20.9542, P < 0.0001) and heavier (F(1, 857) = 585.7293, P < 0.0001) at the Botanical Gardens than at Maden Dam, respectively. The study showed that tubers are an important life stage of S. platyphylla populations and may vary in size and abundance between and within sites. The tuber feeding weevil was shown to develop from egg to ovipositing adult within just over 40 days. Females were recorded to lay up to 48 eggs within a period of one week. Impact studies showed that adult feeding led to a reduction in all but one of the 11 measured plant growth and developmental measurements, including a reduction in the mean mass of the above-ground plant material (F(2,2743) = 12.05, P = 0.002) as well as a reduction in size and abundance of tubers (F(2,58.47) = 9.756, P = 0.0006) and stolons(F(14.943) = 8.7577, P = 0.003). These results are encouraging and suggest that if the insect is released in South Africa, it may prove to be a valuable biocontrol agent. It is concluded that, until suitable biological control options become available in South Africa, the chemical and mechanical control measures currently implemented should continue, however, controlling tubers should be considered during the planning and implementation of these strategies. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
Hermēs Dolios: Performances of Hermes as trickster in the Homeric Hymn to Hermes
- Authors: Nel, Aiden Pieter
- Date: 2021-10-29
- Subjects: Hermes (Greek deity) , Hymn to Hermes , Tricksters in literature , Loki (Norse deity) , Hynes, William J , Doty, William G, 1939-J , Wakdjunkaga (Winnebago Trickster) , Ture (Zande Trickster)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190978 , vital:45046
- Description: This thesis explores the Greek god Hermes' representation in the Homeric Hymn to Hermes to establish the extent to which he partakes in Hynes' defined set of characteristics associated with mythological 'trickster' figures. Hermes is an unorthodox figure as although he embodies many aspects of the trickster, he willingly relinquishes some of these aspects within his hymn. I first outline and explore the trickster phenomenon and the complex methodological issue concerning global comparison. Hynes’ criteria are first applied to three other tricksters the Amerindian (Winnebago) trickster Wakdjunkaga, the central African (Azande) trickster Ture and the northern European (Norse) trickster Loki, which establishes a baseline that will help determine how Hermes conforms to this typology. All three figures’ cultural contexts are first addressed, as the cultural context is key in understanding a figure’s potential inclusion in the trickster category. I then explore Hermes’ performances within his hymn and other genres to formulate how his patron powers and actions relate to his designation as a trickster. I argue, in conclusion, that Hynes’ typology is a useful tool in determining any figure’s degree of ‘tricksterness’ and show that Hermes and the other three figures do embody Hynes’ characteristics. Each, however, embodies them differently and in a specific manner. I argue that the manner in which they relate to the criteria is dictated by their respective cultural contexts, and what their narrators intended to illustrate through the stories wherein tricksters reside. I further argue that narrative intent, genre and cultural context appear to be the main factors that mould each respective figure. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Nel, Aiden Pieter
- Date: 2021-10-29
- Subjects: Hermes (Greek deity) , Hymn to Hermes , Tricksters in literature , Loki (Norse deity) , Hynes, William J , Doty, William G, 1939-J , Wakdjunkaga (Winnebago Trickster) , Ture (Zande Trickster)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190978 , vital:45046
- Description: This thesis explores the Greek god Hermes' representation in the Homeric Hymn to Hermes to establish the extent to which he partakes in Hynes' defined set of characteristics associated with mythological 'trickster' figures. Hermes is an unorthodox figure as although he embodies many aspects of the trickster, he willingly relinquishes some of these aspects within his hymn. I first outline and explore the trickster phenomenon and the complex methodological issue concerning global comparison. Hynes’ criteria are first applied to three other tricksters the Amerindian (Winnebago) trickster Wakdjunkaga, the central African (Azande) trickster Ture and the northern European (Norse) trickster Loki, which establishes a baseline that will help determine how Hermes conforms to this typology. All three figures’ cultural contexts are first addressed, as the cultural context is key in understanding a figure’s potential inclusion in the trickster category. I then explore Hermes’ performances within his hymn and other genres to formulate how his patron powers and actions relate to his designation as a trickster. I argue, in conclusion, that Hynes’ typology is a useful tool in determining any figure’s degree of ‘tricksterness’ and show that Hermes and the other three figures do embody Hynes’ characteristics. Each, however, embodies them differently and in a specific manner. I argue that the manner in which they relate to the criteria is dictated by their respective cultural contexts, and what their narrators intended to illustrate through the stories wherein tricksters reside. I further argue that narrative intent, genre and cultural context appear to be the main factors that mould each respective figure. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
The role of a national system of innovation in facilitating development in South Africa from a comparative BRICS perspective
- Authors: Sibhukwana, Andiswa
- Date: 2022-04-06
- Subjects: BRIC countries , Technological innovations Economic aspects South Africa , Economic development South Africa , Economics Mathematical models , Neoclassical school of economics , South Africa Economic conditions , South Africa Economic policy , National systems of innovation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/284616 , vital:56079
- Description: The aim of the dissertation was to investigate whether the adoption of a national system of innovation has helped facilitate development in South Africa from a comparative BRICS perspective. South Africa has an expanding focus on science and technology, as per the Science and Technology White Paper (1996). There appeared to be innovation that had left out much of the citizenry. There continued to be poverty, inequality, and joblessness. The study aimed to understand how the NSI approach could be used to foster inclusive and transformative development. The study used a mixed-methods approach. The qualitative aspect of the research focused on an innovation and public policy study which assessed the various policies and initiatives implemented in each of the BRICS countries to drive innovation and foster development. The qualitative aspect of the study found that the innovation paradigm required governments to adopt a more holistic approach to public policy design and analysis. The quantitative aspect of the research focused on a trend, correlation, and regression analysis. The trend analysis revealed that China and Brazil increased their allocation of resources towards R&D compared to the other countries. Brazil is regarded as a social investment state, while China is a developmental state: this means the state plays an extraordinarily strong coordinative and financing role in the NSI. On the other hand, the correlation matrix for South Africa revealed a statistically significant positive linear association between various NSI indicators and human development. This suggested that the innovation benefits are trickling down to the general citizenry. In essence the study articulated key elements of the understanding of current and potential impacts of technological change in productivity and growth, employment and inequality that can be used for policy making. , Thesis (MEcon) -- Faculty of Commerce, Economics and Economic History, 2022
- Full Text:
- Date Issued: 2022-04-06
- Authors: Sibhukwana, Andiswa
- Date: 2022-04-06
- Subjects: BRIC countries , Technological innovations Economic aspects South Africa , Economic development South Africa , Economics Mathematical models , Neoclassical school of economics , South Africa Economic conditions , South Africa Economic policy , National systems of innovation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/284616 , vital:56079
- Description: The aim of the dissertation was to investigate whether the adoption of a national system of innovation has helped facilitate development in South Africa from a comparative BRICS perspective. South Africa has an expanding focus on science and technology, as per the Science and Technology White Paper (1996). There appeared to be innovation that had left out much of the citizenry. There continued to be poverty, inequality, and joblessness. The study aimed to understand how the NSI approach could be used to foster inclusive and transformative development. The study used a mixed-methods approach. The qualitative aspect of the research focused on an innovation and public policy study which assessed the various policies and initiatives implemented in each of the BRICS countries to drive innovation and foster development. The qualitative aspect of the study found that the innovation paradigm required governments to adopt a more holistic approach to public policy design and analysis. The quantitative aspect of the research focused on a trend, correlation, and regression analysis. The trend analysis revealed that China and Brazil increased their allocation of resources towards R&D compared to the other countries. Brazil is regarded as a social investment state, while China is a developmental state: this means the state plays an extraordinarily strong coordinative and financing role in the NSI. On the other hand, the correlation matrix for South Africa revealed a statistically significant positive linear association between various NSI indicators and human development. This suggested that the innovation benefits are trickling down to the general citizenry. In essence the study articulated key elements of the understanding of current and potential impacts of technological change in productivity and growth, employment and inequality that can be used for policy making. , Thesis (MEcon) -- Faculty of Commerce, Economics and Economic History, 2022
- Full Text:
- Date Issued: 2022-04-06
Employee views on organisational effectiveness of Chinese organisations operating in Kenya
- Mlotshwa, Minenhle Petronella
- Authors: Mlotshwa, Minenhle Petronella
- Date: 2021-10-29
- Subjects: Organizational effectiveness Kenya , Corporate culture Kenya Cross-cultural studies , China Foreign economic relations Kenya , International business enterprises Kenya , International business enterprises Personnel management , Bilingual communication in organizations Kenya , Religion in the workplace Kenya Cross-cultural studies , Work ethic Kenya Cross-cultural studies
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191898 , vital:45177
- Description: Over the years there has been a growing interest in the relations between Africa and Asia. This is attributed to what is perceived as increasing Chinese influence and presence in Africa. During the past decades, China has experienced rapid economic growth and expansion which has contributed to its need for additional resources and expanding its geographic footprint into Africa and especially Kenya. The growing presence of Chinese organisations in Africa has also faced challenges. Chinese organisations operating in Kenya have been cited as facing cultural challenges, human resource management issues and external pressures which affect Chinese organisations thus impacting organisational effectiveness and performance. While there has been an abundance of research conducted at a macro level, mainly anecdotal knowledge on Chinese organisational values and management, little information exists at the organisation and community level. This study aimed to contribute an in-depth understanding of Chinese and Kenyan employee relationships in organisations at a micro level. Furthermore, this study assesses employees’ views on organisational effectiveness in a cross-cultural research setting of selected Chinese organisations operating in Kenya using the Burke-Litwin model (1992) of organisational effectiveness. This study is qualitative in nature within the interpretivistic research paradigm and used a case study approach to generate detailed comprehensive understanding of the phenomenon under investigation. Two Chinese organisations specialising in importing and exporting of diverse products, which had expanded its operations to Kenya in order to reach Africa’s growing consumer markets were selected for this study. The selected organisations were chosen under the premises that they had been operating in Kenya for more than 20 years, they are reputable organisations in their industries and were international role players. Non-probability convenience sampling was used to select the two organisations who were willing to participate. Purposive sampling was used to identify employees from different job levels and types of work in the selected organisations who were willing to participate in this study. Data was collected by means of face-to-face open-ended semi-structured interviews, with five Chinese and 12 Kenyan employees. The findings of this study were guided by the dimensions of the Burke-Litwin model, comprising the external environment, transformational dimensions (including mission and strategy, leadership and organisational culture) and transactional dimensions (including management practices, organisational structure, systems [policies and procedures], motivation, work climate, task requirements and individual skills and abilities). Based on the findings, organisational culture was the dominant theme that had a large influence on the organisational performance. Within the context of organisational culture, the main challenges faced by employees were religious, work ethic and communication barriers. Leadership and the organisational structure set by senior managers was also impacted by culture. It is evident that the Chinese organisations are dominated by Chinese employees at senior managerial levels – except for one Indian Kenyan manager – and non-managerial levels are dominated by Kenyan employees. Between these two managerial levels there is lack of trust as well as trade union interference favouring Kenyan employees. Leadership of the selected organisations aimed at recruiting candidates who have the necessary skills to contribute toward organisational effectiveness and performance. However, recruited Kenyan employees indicated that they require strategy visibility, effective communication, and structured processes in order to be effective. Despite challenges faced, Chinese and Kenyan employees both strongly agree with and support the efforts of management for providing training and development and creating an environment that accommodates personal growth. The external environment has exerted pressure on the said Chinese organisations. The Kenyan trade unions favour Kenyan employees and place huge pressure on Chinese management. In addition the trade unions bribe locals while making it difficult for Chinese organisations operating in Kenya to conduct business. Through the empirical development of organisational-level research on the case of Chinese organisations operating in Kenya, insightful management knowledge was gathered and shared to assist Chinese and Kenyan employees in their individual and organisational performance. , Thesis (MCom) -- Faculty of Commerce, Management, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Mlotshwa, Minenhle Petronella
- Date: 2021-10-29
- Subjects: Organizational effectiveness Kenya , Corporate culture Kenya Cross-cultural studies , China Foreign economic relations Kenya , International business enterprises Kenya , International business enterprises Personnel management , Bilingual communication in organizations Kenya , Religion in the workplace Kenya Cross-cultural studies , Work ethic Kenya Cross-cultural studies
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/191898 , vital:45177
- Description: Over the years there has been a growing interest in the relations between Africa and Asia. This is attributed to what is perceived as increasing Chinese influence and presence in Africa. During the past decades, China has experienced rapid economic growth and expansion which has contributed to its need for additional resources and expanding its geographic footprint into Africa and especially Kenya. The growing presence of Chinese organisations in Africa has also faced challenges. Chinese organisations operating in Kenya have been cited as facing cultural challenges, human resource management issues and external pressures which affect Chinese organisations thus impacting organisational effectiveness and performance. While there has been an abundance of research conducted at a macro level, mainly anecdotal knowledge on Chinese organisational values and management, little information exists at the organisation and community level. This study aimed to contribute an in-depth understanding of Chinese and Kenyan employee relationships in organisations at a micro level. Furthermore, this study assesses employees’ views on organisational effectiveness in a cross-cultural research setting of selected Chinese organisations operating in Kenya using the Burke-Litwin model (1992) of organisational effectiveness. This study is qualitative in nature within the interpretivistic research paradigm and used a case study approach to generate detailed comprehensive understanding of the phenomenon under investigation. Two Chinese organisations specialising in importing and exporting of diverse products, which had expanded its operations to Kenya in order to reach Africa’s growing consumer markets were selected for this study. The selected organisations were chosen under the premises that they had been operating in Kenya for more than 20 years, they are reputable organisations in their industries and were international role players. Non-probability convenience sampling was used to select the two organisations who were willing to participate. Purposive sampling was used to identify employees from different job levels and types of work in the selected organisations who were willing to participate in this study. Data was collected by means of face-to-face open-ended semi-structured interviews, with five Chinese and 12 Kenyan employees. The findings of this study were guided by the dimensions of the Burke-Litwin model, comprising the external environment, transformational dimensions (including mission and strategy, leadership and organisational culture) and transactional dimensions (including management practices, organisational structure, systems [policies and procedures], motivation, work climate, task requirements and individual skills and abilities). Based on the findings, organisational culture was the dominant theme that had a large influence on the organisational performance. Within the context of organisational culture, the main challenges faced by employees were religious, work ethic and communication barriers. Leadership and the organisational structure set by senior managers was also impacted by culture. It is evident that the Chinese organisations are dominated by Chinese employees at senior managerial levels – except for one Indian Kenyan manager – and non-managerial levels are dominated by Kenyan employees. Between these two managerial levels there is lack of trust as well as trade union interference favouring Kenyan employees. Leadership of the selected organisations aimed at recruiting candidates who have the necessary skills to contribute toward organisational effectiveness and performance. However, recruited Kenyan employees indicated that they require strategy visibility, effective communication, and structured processes in order to be effective. Despite challenges faced, Chinese and Kenyan employees both strongly agree with and support the efforts of management for providing training and development and creating an environment that accommodates personal growth. The external environment has exerted pressure on the said Chinese organisations. The Kenyan trade unions favour Kenyan employees and place huge pressure on Chinese management. In addition the trade unions bribe locals while making it difficult for Chinese organisations operating in Kenya to conduct business. Through the empirical development of organisational-level research on the case of Chinese organisations operating in Kenya, insightful management knowledge was gathered and shared to assist Chinese and Kenyan employees in their individual and organisational performance. , Thesis (MCom) -- Faculty of Commerce, Management, 2021
- Full Text:
- Date Issued: 2021-10-29
The economic valuation of ecosystem services using deliberation as a tool for value elicitation
- Authors: Marriner, James Derek
- Date: 2021-10-29
- Subjects: Seagrasses Economic aspects South Africa , Ecosystem services South Africa , Focus groups , Deliberative democracy South Africa , Nature Effect of human beings on South Africa , Environmental economics , Contingent valuation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/294712 , vital:57247
- Description: Marine seagrass, Zostera capensis, is a highly productive flowering marine seagrass found on the eastern and southern coasts of South Africa and provides a variety of ecosystem services, which include reducing the effects of erosion, trapping nutrients, creating a nursery habitat for fish species and reducing sedimentation. Despite their formal protection status, Z. capensis meadows are regressing, often due to anthropogenic influences. Globally, multiple studies have documented the ecological importance of seagrass services, to both humans and nature, but economic evaluations of these services have not been as extensive. Numerous valuation methods have been used in literature and in practice to value non-market environmental resources and ecosystem services, where results vary. The most widely used non-market environmental valuation method is the contingent valuation (CV) method, which allows for the valuation of environmental resources that are not sold in the market through a stated willingness to pay (WTP) amount, contingent upon a particular scenario. However, the CV method is susceptible to various limitations and forms of bias. As a result, alternative environmental valuation techniques have been reviewed in literature. Deliberation has been suggested as an improved valuation approach to overcome the criticisms of the CV technique, as it increases respondents’ understanding and knowledge of the environmental resource under discussion, through the incorporation of debate, discussion, participation and social learning, thereby producing more reliable valuations of non-market environmental resources. The deliberative monetary valuation (DMV) method has been advocated as an important valuation method to achieve more comprehensive and reliable valuations of complex and unfamiliar public goods, such as ecosystem services. This thesis explored the determinants of WTP for the protection of seagrass, and whether deliberation can be used to supplement findings and values obtained from the individually sourced WTP values. As a result, the study used a dual-method approach to obtain both individual and deliberated WTP values from a range of stakeholders with varied socio-demographic characteristics by using both the CV method and a deliberative focus group, for the ecosystem services that seagrass Z. capensis provides to the Knysna community, South Africa. The results showed that age, education, use frequency and mistrust in local government were determinants in WTP, while deliberative elements uncovered that respondents’ knowledge of an environmental resource and a consideration of the valuation scenario were important factors in determining WTP. It was found that 55% of the respondents were willing to pay to conserve the ecosystem services of seagrass in the Knysna estuary through a monitoring programme. Among those that were willing, the mean WTP amount was approximately R132 per month per household. Although the circumstances in which the deliberative focus group discussion were run were not ideal because of the COVID-19 lockdown, the results showed some interesting potential benefits of using deliberation in complex public good valuation studies. , Thesis (MEcon) -- Faculty of Commerce, Economics and Economic History, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Marriner, James Derek
- Date: 2021-10-29
- Subjects: Seagrasses Economic aspects South Africa , Ecosystem services South Africa , Focus groups , Deliberative democracy South Africa , Nature Effect of human beings on South Africa , Environmental economics , Contingent valuation
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/294712 , vital:57247
- Description: Marine seagrass, Zostera capensis, is a highly productive flowering marine seagrass found on the eastern and southern coasts of South Africa and provides a variety of ecosystem services, which include reducing the effects of erosion, trapping nutrients, creating a nursery habitat for fish species and reducing sedimentation. Despite their formal protection status, Z. capensis meadows are regressing, often due to anthropogenic influences. Globally, multiple studies have documented the ecological importance of seagrass services, to both humans and nature, but economic evaluations of these services have not been as extensive. Numerous valuation methods have been used in literature and in practice to value non-market environmental resources and ecosystem services, where results vary. The most widely used non-market environmental valuation method is the contingent valuation (CV) method, which allows for the valuation of environmental resources that are not sold in the market through a stated willingness to pay (WTP) amount, contingent upon a particular scenario. However, the CV method is susceptible to various limitations and forms of bias. As a result, alternative environmental valuation techniques have been reviewed in literature. Deliberation has been suggested as an improved valuation approach to overcome the criticisms of the CV technique, as it increases respondents’ understanding and knowledge of the environmental resource under discussion, through the incorporation of debate, discussion, participation and social learning, thereby producing more reliable valuations of non-market environmental resources. The deliberative monetary valuation (DMV) method has been advocated as an important valuation method to achieve more comprehensive and reliable valuations of complex and unfamiliar public goods, such as ecosystem services. This thesis explored the determinants of WTP for the protection of seagrass, and whether deliberation can be used to supplement findings and values obtained from the individually sourced WTP values. As a result, the study used a dual-method approach to obtain both individual and deliberated WTP values from a range of stakeholders with varied socio-demographic characteristics by using both the CV method and a deliberative focus group, for the ecosystem services that seagrass Z. capensis provides to the Knysna community, South Africa. The results showed that age, education, use frequency and mistrust in local government were determinants in WTP, while deliberative elements uncovered that respondents’ knowledge of an environmental resource and a consideration of the valuation scenario were important factors in determining WTP. It was found that 55% of the respondents were willing to pay to conserve the ecosystem services of seagrass in the Knysna estuary through a monitoring programme. Among those that were willing, the mean WTP amount was approximately R132 per month per household. Although the circumstances in which the deliberative focus group discussion were run were not ideal because of the COVID-19 lockdown, the results showed some interesting potential benefits of using deliberation in complex public good valuation studies. , Thesis (MEcon) -- Faculty of Commerce, Economics and Economic History, 2021
- Full Text:
- Date Issued: 2021-10-29
The well-being implications of Urban Natures among Black Urban Dwellers in the Eastern Cape
- Authors: Walsh, Lindsey Sara
- Date: 2021-10-29
- Subjects: Apartheid South Africa Eastern Cape , Decolonization South Africa Eastern Cape , Urban Black people South Africa Eastern Cape , Quality of life South Africa Eastern Cape , Well-being South Africa Eastern Cape , Rural-urban migration South Africa Eastern Cape , Urban ecology (Sociology) South Africa Eastern Cape , Land use, Urban South Africa Eastern Cape , City and town life South Africa Eastern Cape , Sociology, Urban South Africa Eastern Cape , Nature Social aspects South Africa Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192966 , vital:45285
- Description: Throughout the world, urbanisation is at an all-time high. It is estimated that two-thirds of the global population will be living in urban centres by 2030. The highest urban growth rates are currently found in sub -Saharan Africa, however, approximately 70% of urban residents in Sub-Saharan Africa reside in informal settlements. Such processes have resulted in reduced areas of natural space and biodiversity. While the benefits of exposure to and experiences of nature to human well-being and quality of life are increasingly being acknowledged, these links are largely understood through a western lens, where nature and culture are dichotomised. The implications of this are that the types of nature(s) that are made available in urban areas are designed to provide opportunities for urban residents to only engage in recreational and relaxation activities within such places. However, among indigenous and non-westernised communities, culture is a dynamic and holistic entity, deeply rooted in the constructions of nature. Very few studies from the global south have entered this dialogue. In response, this study, conducted amongst Xhosa speaking urban residents living in two medium-sized towns, was undertaken to explore the connections and meanings they attributed to nature(s) and how their engagement in such places contributed to their sense of well-being. This study found that strong connections and meanings to nature still exist. Many of these relate to personal experiences which are given meaning through local understandings and associations which are deeply embedded within cultural practices and rituals. Engagement and or memories of such are strongly associated with a sense of well-being often only experienced in rural areas. In such contexts, well-being is also understood as a holistic concept that is underpinned by these nature-culture connections. Within an urban context, the benefits of such connections are hindered by the types of nature(s) found in these areas as well as high levels of crime and the poor management of such places. , Thesis (MA) -- Faculty of Humanities, Anthropology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Walsh, Lindsey Sara
- Date: 2021-10-29
- Subjects: Apartheid South Africa Eastern Cape , Decolonization South Africa Eastern Cape , Urban Black people South Africa Eastern Cape , Quality of life South Africa Eastern Cape , Well-being South Africa Eastern Cape , Rural-urban migration South Africa Eastern Cape , Urban ecology (Sociology) South Africa Eastern Cape , Land use, Urban South Africa Eastern Cape , City and town life South Africa Eastern Cape , Sociology, Urban South Africa Eastern Cape , Nature Social aspects South Africa Eastern Cape
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192966 , vital:45285
- Description: Throughout the world, urbanisation is at an all-time high. It is estimated that two-thirds of the global population will be living in urban centres by 2030. The highest urban growth rates are currently found in sub -Saharan Africa, however, approximately 70% of urban residents in Sub-Saharan Africa reside in informal settlements. Such processes have resulted in reduced areas of natural space and biodiversity. While the benefits of exposure to and experiences of nature to human well-being and quality of life are increasingly being acknowledged, these links are largely understood through a western lens, where nature and culture are dichotomised. The implications of this are that the types of nature(s) that are made available in urban areas are designed to provide opportunities for urban residents to only engage in recreational and relaxation activities within such places. However, among indigenous and non-westernised communities, culture is a dynamic and holistic entity, deeply rooted in the constructions of nature. Very few studies from the global south have entered this dialogue. In response, this study, conducted amongst Xhosa speaking urban residents living in two medium-sized towns, was undertaken to explore the connections and meanings they attributed to nature(s) and how their engagement in such places contributed to their sense of well-being. This study found that strong connections and meanings to nature still exist. Many of these relate to personal experiences which are given meaning through local understandings and associations which are deeply embedded within cultural practices and rituals. Engagement and or memories of such are strongly associated with a sense of well-being often only experienced in rural areas. In such contexts, well-being is also understood as a holistic concept that is underpinned by these nature-culture connections. Within an urban context, the benefits of such connections are hindered by the types of nature(s) found in these areas as well as high levels of crime and the poor management of such places. , Thesis (MA) -- Faculty of Humanities, Anthropology, 2021
- Full Text:
- Date Issued: 2021-10-29
The bastards of Euripides: illegitimacy in the Hippolytus, the Andromache, and the Ion
- Sülter, James Nicholas Edwin
- Authors: Sülter, James Nicholas Edwin
- Date: 2021-10-29
- Subjects: Euripides , Euripides Criticism and interpretation , Euripides. Hippolytus , Euripides. Andromache , Euripides. Ion , Illegitimacy in literature , Tragedy History and criticism , Citizenship in literature
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190030 , vital:44957
- Description: The Pericles Citizenship Law of 451 made citizenship contingent on an individual man having Athenian blood on both his father and mother’s side. Men with foreign mothers were denied any rights to the key institutions that conferred Athenian belonging, such as deme and phratry membership, as well as, of course, citizenship. When, however, the Peloponnesian War began in 431, the population of Athenian citizens was quickly diminished by military conflict and plague and they sought a solution to repopulate their numbers with as little change to the original law as possible. The result was an amendment in 429 allowing citizens with no living legitimate (γνήσιοι) heirs to legitimise their bastards (νόθοι) in their place. A year after this, Euripides wrote the Hippolytus, a play that deals very closely with the issues of bastardy, bastards’ place in the polis and the oikos, and their treatment by those who are legitimate. As the war went on, he proceeded to produce two other plays containing bastard characters, the Andromache (c.425) and the Ion (c.413), in which he similarly explored the consequences of illegitimacy. In all three plays his attitude towards bastards is overwhelmingly sympathetic and the way he chooses to present their situations suggests his disagreement with the arrogance and antipathy that continued to push many of these often very worthy individuals to the fringes of society. This thesis seeks to examine Euripides’ presentation of bastards through a close reading of the relevant texts, in order to understand how the plays reacted to the contemporary circumstances of and what comments they are making. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Sülter, James Nicholas Edwin
- Date: 2021-10-29
- Subjects: Euripides , Euripides Criticism and interpretation , Euripides. Hippolytus , Euripides. Andromache , Euripides. Ion , Illegitimacy in literature , Tragedy History and criticism , Citizenship in literature
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190030 , vital:44957
- Description: The Pericles Citizenship Law of 451 made citizenship contingent on an individual man having Athenian blood on both his father and mother’s side. Men with foreign mothers were denied any rights to the key institutions that conferred Athenian belonging, such as deme and phratry membership, as well as, of course, citizenship. When, however, the Peloponnesian War began in 431, the population of Athenian citizens was quickly diminished by military conflict and plague and they sought a solution to repopulate their numbers with as little change to the original law as possible. The result was an amendment in 429 allowing citizens with no living legitimate (γνήσιοι) heirs to legitimise their bastards (νόθοι) in their place. A year after this, Euripides wrote the Hippolytus, a play that deals very closely with the issues of bastardy, bastards’ place in the polis and the oikos, and their treatment by those who are legitimate. As the war went on, he proceeded to produce two other plays containing bastard characters, the Andromache (c.425) and the Ion (c.413), in which he similarly explored the consequences of illegitimacy. In all three plays his attitude towards bastards is overwhelmingly sympathetic and the way he chooses to present their situations suggests his disagreement with the arrogance and antipathy that continued to push many of these often very worthy individuals to the fringes of society. This thesis seeks to examine Euripides’ presentation of bastards through a close reading of the relevant texts, in order to understand how the plays reacted to the contemporary circumstances of and what comments they are making. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
- Full Text:
- Date Issued: 2021-10-29
Green Synthesis of HIV-1 Protease Inhibitors
- Authors: Hartley, Shaun Neil
- Date: 2021-10
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190145 , vital:44967
- Description: Thesis embargoed until October 2022 , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Hartley, Shaun Neil
- Date: 2021-10
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190145 , vital:44967
- Description: Thesis embargoed until October 2022 , Thesis (MSc) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10
Evaluation of Megabruchidius tonkineus (Coleoptera: Chrysomelidae: Bruchinae), a candidate biological control agent for Gleditsia triacanthos L. (Fabaceae) in South Africa
- Salgado Astudillo, Sara Elizabeth
- Authors: Salgado Astudillo, Sara Elizabeth
- Date: 2021-10
- Subjects: Honey locust South Africa , Honey locust Biological control South Africa , Invasive plants Biological control South Africa , Biogeography South Africa , Biogeography Climatic factors South Africa , Megabruchidius tonkineus South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/188386 , vital:44749
- Description: Gleditsia triacanthos L. (Fabaceae) (honey locust) is a fast-growing, deciduous tree indigenous to the United States of America. Introduced around the world as an ornamental tree, it has become invasive in a number of countries. Where it is invasive, G. triacanthos competes and replaces indigenous species; it creates dense stands along watercourses, posing a significant environmental threat. In South Africa, G. triacanthos is regarded as one of the country’s fastest spreading weeds. Gleditsia triacanthos produces numerous seeds contained in large hanging pods. Once dislodged from the pods, the seeds are dispersed by birds and mammals, including livestock, which eat the pods. It has been suggested that the seeds should be the target for biological control programme. Some invasive alien plant species are characterised by their ability to spread and establish in new ecosystems because they tolerate a wide range of environmental conditions. In order to predict areas of likely invasion, species distribution models (SDMs) are used to identify areas climatically suitable for their invasion, so enabling better targeted control of the plant species. Gleditsia triacanthos adapts to a wide range of climates and soil types, and tolerates salinity, drought and frost. Currently primarily restricted to the Grassland Biome of South Africa, G. triacanthos has doubled its distribution area in the past 15 years, and it is not known how far the species will spread. In this study we used two different modelling programmes, CLIMEX and MaxEnt, to predict areas where G. triacanthos could find favourable growing conditions; both SDMs showed that most of the country is suitable for G. triacanthos and that it will probably continue to spread, if left unmanaged, into new bioregions, such as the Karoo. In South Africa, the Asian seed-feeding bruchid, Megabruchidius tonkineus (Pic, 1914) (Coleoptera: Chrysomelidae: Bruchinae) has been recorded in the plant’s seed pods and has been considered as a biological control agent. The insect was not released as part of a formal biological control programme and neither host-specificity nor impact studies were conducted on the species prior to its discovery. In 2017 a decision was made to re-consider its status as a Abstract biological control agent until further details of its biology, host specificity, and impact on the seeds of G. triacanthos in South Africa were available. This study shows that Megabruchidius tonkineus has established across the entire G. triacanthos population in South Africa damaging approximately 9% of seeds. Laboratory studies show that, Megabruchidius tonkineus completes its larval development in the seeds of G. triacanthos in about 66.80 ± 0.6880 SE days before eclosing. In addition, the adult females oviposit on the following Fabaceae species: Arachis hypogaea, Albizia, julibrissin, Cicer arietinum, Pisum sativum, Dipogon lignosus, Peltophorum africanum, Podalyria buxifolia Senegalia burkei, Umtiza listerina and Vachellia sieberiana. However, larval development was limited to G. triacanthos. It is concluded that the seed-feeding beetle is not a threat to native Fabaceae species in South Africa, however, it does not damage enough G. triacanthos seeds to be considered a valuable biological control agent at this stage, and additional seed-feeding biological control agents should be considered to reduce the number of G. triacanthos seeds entering the environment. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Salgado Astudillo, Sara Elizabeth
- Date: 2021-10
- Subjects: Honey locust South Africa , Honey locust Biological control South Africa , Invasive plants Biological control South Africa , Biogeography South Africa , Biogeography Climatic factors South Africa , Megabruchidius tonkineus South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/188386 , vital:44749
- Description: Gleditsia triacanthos L. (Fabaceae) (honey locust) is a fast-growing, deciduous tree indigenous to the United States of America. Introduced around the world as an ornamental tree, it has become invasive in a number of countries. Where it is invasive, G. triacanthos competes and replaces indigenous species; it creates dense stands along watercourses, posing a significant environmental threat. In South Africa, G. triacanthos is regarded as one of the country’s fastest spreading weeds. Gleditsia triacanthos produces numerous seeds contained in large hanging pods. Once dislodged from the pods, the seeds are dispersed by birds and mammals, including livestock, which eat the pods. It has been suggested that the seeds should be the target for biological control programme. Some invasive alien plant species are characterised by their ability to spread and establish in new ecosystems because they tolerate a wide range of environmental conditions. In order to predict areas of likely invasion, species distribution models (SDMs) are used to identify areas climatically suitable for their invasion, so enabling better targeted control of the plant species. Gleditsia triacanthos adapts to a wide range of climates and soil types, and tolerates salinity, drought and frost. Currently primarily restricted to the Grassland Biome of South Africa, G. triacanthos has doubled its distribution area in the past 15 years, and it is not known how far the species will spread. In this study we used two different modelling programmes, CLIMEX and MaxEnt, to predict areas where G. triacanthos could find favourable growing conditions; both SDMs showed that most of the country is suitable for G. triacanthos and that it will probably continue to spread, if left unmanaged, into new bioregions, such as the Karoo. In South Africa, the Asian seed-feeding bruchid, Megabruchidius tonkineus (Pic, 1914) (Coleoptera: Chrysomelidae: Bruchinae) has been recorded in the plant’s seed pods and has been considered as a biological control agent. The insect was not released as part of a formal biological control programme and neither host-specificity nor impact studies were conducted on the species prior to its discovery. In 2017 a decision was made to re-consider its status as a Abstract biological control agent until further details of its biology, host specificity, and impact on the seeds of G. triacanthos in South Africa were available. This study shows that Megabruchidius tonkineus has established across the entire G. triacanthos population in South Africa damaging approximately 9% of seeds. Laboratory studies show that, Megabruchidius tonkineus completes its larval development in the seeds of G. triacanthos in about 66.80 ± 0.6880 SE days before eclosing. In addition, the adult females oviposit on the following Fabaceae species: Arachis hypogaea, Albizia, julibrissin, Cicer arietinum, Pisum sativum, Dipogon lignosus, Peltophorum africanum, Podalyria buxifolia Senegalia burkei, Umtiza listerina and Vachellia sieberiana. However, larval development was limited to G. triacanthos. It is concluded that the seed-feeding beetle is not a threat to native Fabaceae species in South Africa, however, it does not damage enough G. triacanthos seeds to be considered a valuable biological control agent at this stage, and additional seed-feeding biological control agents should be considered to reduce the number of G. triacanthos seeds entering the environment. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
Development of a 3D bioprinting and standalone bioreactor unit for the production and maintenance of bioscaffolds in vitro
- Authors: Hundling, Jethro Daniel
- Date: 2021-10-29
- Subjects: Bioreactors , Tissue scaffolds , Cell culture , Polyethylene glycol Biotechnology , 3D bioprinting , Poly(ethylene glycol) diacrylate (PEGDA)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192063 , vital:45192
- Description: The most common method for in vitro cell culture currently is to grow a specific cell type in isolation, in monolayer format, adhered to a 2D culture surface. This brings about many limitations in comparison to in vivo models due to altered cell phenotypes, as caused by the culturing technique itself, and the lack of naturally occurring cell-to-cell interactions. Three dimensional mammalian cell culture technologies have the potential to overcome these limitations, and provide models more representative of natural systems. Unfortunately, the cost and difficulty associated with achieving sustainable and useful 3D mammalian cell culture is still very high, preventing its widespread adoption across scientific platforms. In this research, we investigate the feasibility of developing and producing a visible light-based 3D stereolithographic bioprinter to produce 3D scaffolds for cell culture. Furthermore, we investigate the possibility of developing and implementing a forced perfusion bioreactor system, which would support the produced scaffold and improve longer-term culture conditions. The developed 3D bioprinter, and bioreactor designs were developed and tested alongside Poly (ethylene glycol) diacrylate (PEGDA), a versatile synthetic scaffold material. PEGDA itself was also evaluated for its printability, robustness in culture conditions over time, and its ability to maintain 3D mammalian cell culture. This research showed that both the developed 3D bioprinter, and bioreactor unit were capable of producing and maintaining an easily modifiable PEGDA scaffold, in culture conditions. In addition, the PEGDA formulation developed was shown to allow for the effective and reproducible 3D cell culture conditions over the medium term, with automated media feeding. The research presented here aimed to illustrate a proof of concept that the low-cost development and production of 3D culture scaffold production and maintenance systems was feasible to the scientific research environment. This technology can then be built upon, into a system that would then allow for the broader adoption and investigation of 3D cell culture as a tool within the scientific community. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Hundling, Jethro Daniel
- Date: 2021-10-29
- Subjects: Bioreactors , Tissue scaffolds , Cell culture , Polyethylene glycol Biotechnology , 3D bioprinting , Poly(ethylene glycol) diacrylate (PEGDA)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/192063 , vital:45192
- Description: The most common method for in vitro cell culture currently is to grow a specific cell type in isolation, in monolayer format, adhered to a 2D culture surface. This brings about many limitations in comparison to in vivo models due to altered cell phenotypes, as caused by the culturing technique itself, and the lack of naturally occurring cell-to-cell interactions. Three dimensional mammalian cell culture technologies have the potential to overcome these limitations, and provide models more representative of natural systems. Unfortunately, the cost and difficulty associated with achieving sustainable and useful 3D mammalian cell culture is still very high, preventing its widespread adoption across scientific platforms. In this research, we investigate the feasibility of developing and producing a visible light-based 3D stereolithographic bioprinter to produce 3D scaffolds for cell culture. Furthermore, we investigate the possibility of developing and implementing a forced perfusion bioreactor system, which would support the produced scaffold and improve longer-term culture conditions. The developed 3D bioprinter, and bioreactor designs were developed and tested alongside Poly (ethylene glycol) diacrylate (PEGDA), a versatile synthetic scaffold material. PEGDA itself was also evaluated for its printability, robustness in culture conditions over time, and its ability to maintain 3D mammalian cell culture. This research showed that both the developed 3D bioprinter, and bioreactor unit were capable of producing and maintaining an easily modifiable PEGDA scaffold, in culture conditions. In addition, the PEGDA formulation developed was shown to allow for the effective and reproducible 3D cell culture conditions over the medium term, with automated media feeding. The research presented here aimed to illustrate a proof of concept that the low-cost development and production of 3D culture scaffold production and maintenance systems was feasible to the scientific research environment. This technology can then be built upon, into a system that would then allow for the broader adoption and investigation of 3D cell culture as a tool within the scientific community. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2021
- Full Text:
- Date Issued: 2021-10-29