Biological longitudinal aspects of the Kabompo River, a significant tributary of the upper Zambezi sub-catchment, North-west Province, Zambia
- Janse van Rensburg, Lomarie Cathleen
- Authors: Janse van Rensburg, Lomarie Cathleen
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422292 , vital:71928
- Description: The landscape of the Upper Zambezi Catchment in the remote North-Western Province of Zambia retains many natural features, but current and future anthropogenic activities threaten its integrity. Lack of information relating to the basic ecological functioning of the region hampers conservation efforts. Flooding from the adjacent woody-savanna and open grasslands in the Upper-Zambezi Catchment in the wet season drives crucial processes in the aquatic ecosystems such as the Kabompo River. This thesis describes aquatic food webs and describes the nutrient cycling in this river. A general introduction was based on the seasonality, river morphology, and biodiversity of the Kabompo River. There is no aquatic food web information available on this river. To address this knowledge gap, sampling of the principal food web components– vegetation, invertebrates, and fish – were collected in the dry season from August to September 2019 across six sites in the Kabompo River. Stable isotope analyses provided proxies for the food web structure at each site, and were the basis of longitudinal comparisons. The analyses suggest that the Kabompo River food webs follow the trophic ordination and nutrient cycling characteristic of the revised-Riverine Productivity Model. Food webs and community assemblages remain structurally similar between sites and provide some evidence of bottom-up productivity-driven trophic dynamics. To predict the possible landscape-scale processing of nutrient changes present for the aquatic ecosystems of the Kabompo River, a longitudinal comparison between a herbivore, Labeo cylindricus, and omnivore, Synodontis spp., fish species and their resources (primary producers and invertebrates) was done and showed archetypal fish food web trophic separation (2 ‰ to3 ‰) between species which remains consistent for the headwater sites. The nutrient values change at the convergence between the Kabompo Bridge and the Mwinilunga branches and show a decrease in the trophic separation of the sites below. This change becomes apparent from the last site, Watopa, where the trophic separation re-establishes (2 ‰ to 3 ‰) between the species. The trend suggests primarily autochthonous production in the headwater reaches, changing to allochthonous and autochthonous nutrient incorporation after the convergence of the two main branches. Permanent wetlands surrounding the convergence zones may be conducive to more primary producer activity and increased nutrient turnover. Permanent wetlands are a common feature of the Upper Zambezi Catchment tributaries, with the most notable lentic system the Barotse Flood Plains in the south, which shares reach-adjacent characteristics with the Kabompo River. Trends from the 2015 dry season identified for the Barotse Flood Plains support the findings of this thesis, where the δ13C values recorded reach depletion of -45 ‰. Management in the Upper Zambezi Basin and the Kabompo River comprises biannual assessments of water quality and quantity by the Zambezi River Authority, but not of the ecosystem process. This thesis may provide information to address the ecological (food webs and nutrient cycling) dynamics of the Basin rivers. As the first baseline information on the river's biology and ecology, it may present a comparative basis for future assessments under conservation management strategies. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Janse van Rensburg, Lomarie Cathleen
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422292 , vital:71928
- Description: The landscape of the Upper Zambezi Catchment in the remote North-Western Province of Zambia retains many natural features, but current and future anthropogenic activities threaten its integrity. Lack of information relating to the basic ecological functioning of the region hampers conservation efforts. Flooding from the adjacent woody-savanna and open grasslands in the Upper-Zambezi Catchment in the wet season drives crucial processes in the aquatic ecosystems such as the Kabompo River. This thesis describes aquatic food webs and describes the nutrient cycling in this river. A general introduction was based on the seasonality, river morphology, and biodiversity of the Kabompo River. There is no aquatic food web information available on this river. To address this knowledge gap, sampling of the principal food web components– vegetation, invertebrates, and fish – were collected in the dry season from August to September 2019 across six sites in the Kabompo River. Stable isotope analyses provided proxies for the food web structure at each site, and were the basis of longitudinal comparisons. The analyses suggest that the Kabompo River food webs follow the trophic ordination and nutrient cycling characteristic of the revised-Riverine Productivity Model. Food webs and community assemblages remain structurally similar between sites and provide some evidence of bottom-up productivity-driven trophic dynamics. To predict the possible landscape-scale processing of nutrient changes present for the aquatic ecosystems of the Kabompo River, a longitudinal comparison between a herbivore, Labeo cylindricus, and omnivore, Synodontis spp., fish species and their resources (primary producers and invertebrates) was done and showed archetypal fish food web trophic separation (2 ‰ to3 ‰) between species which remains consistent for the headwater sites. The nutrient values change at the convergence between the Kabompo Bridge and the Mwinilunga branches and show a decrease in the trophic separation of the sites below. This change becomes apparent from the last site, Watopa, where the trophic separation re-establishes (2 ‰ to 3 ‰) between the species. The trend suggests primarily autochthonous production in the headwater reaches, changing to allochthonous and autochthonous nutrient incorporation after the convergence of the two main branches. Permanent wetlands surrounding the convergence zones may be conducive to more primary producer activity and increased nutrient turnover. Permanent wetlands are a common feature of the Upper Zambezi Catchment tributaries, with the most notable lentic system the Barotse Flood Plains in the south, which shares reach-adjacent characteristics with the Kabompo River. Trends from the 2015 dry season identified for the Barotse Flood Plains support the findings of this thesis, where the δ13C values recorded reach depletion of -45 ‰. Management in the Upper Zambezi Basin and the Kabompo River comprises biannual assessments of water quality and quantity by the Zambezi River Authority, but not of the ecosystem process. This thesis may provide information to address the ecological (food webs and nutrient cycling) dynamics of the Basin rivers. As the first baseline information on the river's biology and ecology, it may present a comparative basis for future assessments under conservation management strategies. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Coastal movements and estuarine use of sub-adult and adult leervis, lichia amia: results from long-term acoustic tracking
- Authors: Mxo, Rebecca Vuyolwethu
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422314 , vital:71930
- Description: Information on fish movement is important not only for understanding a species’ ecological importance, but also for developing appropriate conservation and management policies that are critical for food security and biodiversity preservation. This information is particularly important for species that occupy different habitats at different life history stages, and display predictable movement patterns, such as an annual spawning migration. Leervis Lichia amia is an estuary-dependent fishery species of high ecological and recreational importance in South Africa. There has been a steady decline in catch-per-unit-effort in the marine recreational fishery for this species over the past 20 years, and the most recent stock assessment classified the adult stock as collapsed. This study investigates L. amia multi-year coastal migrations and estuarine habitat use of sub-adult and adult fish tagged with long-life acoustic transmitters Seventy-eight L. amia (two juveniles, fifty-four subadults, and twenty-one adults) were tagged throughout their South African distribution and monitored between 2011 and 2020 producing a decade long dataset. Results show that regardless of the tagging region, clear migration patterns were observed, demonstrating that both sub-adult and adult L. amia migrate annually to KZN in the austral winter and predictably return to the WC and EC waters in the summer. The likelihood of partial migration was also identified, with the coexistence of migratory and resident behaviors within a single L. amia population. In addition, Overwintering behaviour was also observed with L. amia adults that remained resident throughout the year, foregoing the annual migration, phenomenon known as skipped spawning, and homing behaviour, where L. amia, particularly those tagged in the EC and WC, were recorded returning to previously occupied tagging locations and surrounding areas. The importance of estuaries to sub-adult and adult fish was also assessed and identified the importance of estuaries not only to subadults but also to adults. Estuary visits were strongly influenced by the environment which the fish was tagged in, temporal and seasonal changes, and life-history stages. The predictability of their migrations (almost to the day), the varied migratory behaviour (overwintering), returning to sites of familiarity post-migration, and long-term dependency on estuaries even as sub-adults and adults, provide motivation for increased protection of this species, including extending the network of estuarine protected areas in the country, and a closed fishing season, particularly during the annual winter migration. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Mxo, Rebecca Vuyolwethu
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422314 , vital:71930
- Description: Information on fish movement is important not only for understanding a species’ ecological importance, but also for developing appropriate conservation and management policies that are critical for food security and biodiversity preservation. This information is particularly important for species that occupy different habitats at different life history stages, and display predictable movement patterns, such as an annual spawning migration. Leervis Lichia amia is an estuary-dependent fishery species of high ecological and recreational importance in South Africa. There has been a steady decline in catch-per-unit-effort in the marine recreational fishery for this species over the past 20 years, and the most recent stock assessment classified the adult stock as collapsed. This study investigates L. amia multi-year coastal migrations and estuarine habitat use of sub-adult and adult fish tagged with long-life acoustic transmitters Seventy-eight L. amia (two juveniles, fifty-four subadults, and twenty-one adults) were tagged throughout their South African distribution and monitored between 2011 and 2020 producing a decade long dataset. Results show that regardless of the tagging region, clear migration patterns were observed, demonstrating that both sub-adult and adult L. amia migrate annually to KZN in the austral winter and predictably return to the WC and EC waters in the summer. The likelihood of partial migration was also identified, with the coexistence of migratory and resident behaviors within a single L. amia population. In addition, Overwintering behaviour was also observed with L. amia adults that remained resident throughout the year, foregoing the annual migration, phenomenon known as skipped spawning, and homing behaviour, where L. amia, particularly those tagged in the EC and WC, were recorded returning to previously occupied tagging locations and surrounding areas. The importance of estuaries to sub-adult and adult fish was also assessed and identified the importance of estuaries not only to subadults but also to adults. Estuary visits were strongly influenced by the environment which the fish was tagged in, temporal and seasonal changes, and life-history stages. The predictability of their migrations (almost to the day), the varied migratory behaviour (overwintering), returning to sites of familiarity post-migration, and long-term dependency on estuaries even as sub-adults and adults, provide motivation for increased protection of this species, including extending the network of estuarine protected areas in the country, and a closed fishing season, particularly during the annual winter migration. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Crop fields abandonment: assessing the dynamics of degradation in relation to leverage points for sustainable land management in the Macubeni catchment, South Africa
- Authors: Sibiya, Silindile
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422470 , vital:71945
- Description: Soil erosion is a major global environmental problem and a pervasive forms of land degradation that threatens land productivity and food and water security. Some of the biggest sources of sediment in catchments are previously cultivated lands. Regardless of this factor, the abandonment of cultivated fields is not well-researched. Sustainable land management (SLM) interventions can play a significant role in mitigating and halting land degradation. This study investigated the dynamics of degradation exhibited by crop fields and the potential impacts of SLM interventions, using a leverage points framework and a case study in the Macubeni catchment of South Africa. The research answers three questions: (1) What is the relationship between the usage status of crop fields and degradation in Macubeni? (2) What are the drivers of crop field abandonment and how do they interact in the system? (3) Can proposed sustainable land management interventions tackle the dynamics of land abandonment, and associated degradation, at the root cause level? An empirical-analytical approach using a four step multi-method process was followed, in which crop fields were mapped using ArcGIS tools, literature was reviewed alongside stakeholder engagements, qualitative systems mapping modelling was undertaken, and a Multi-Criteria Analysis (MCA) with leverage points hierarchy was used to integrate all the steps together. The results revealed that the various drivers of crop field abandonment include natural environmental factors, socio-economic and social factors. 47.41% of the total crop fields in Macubeni were classified as highly degraded, and abandoned fields covered 37.47%. The statistical Chi-Square Test also confirmed that there is a significant relationship between the usage status and degradation level in crop fields. The SLM interventions assessed in the study have the potential to tackle the dynamics of land abandonment at a root cause level, however, there is a need to first shift the community’s mental models to address the existing sources of change resistance that are hindering successful implementation. Furthermore, the innovative multi-method approach applied in this study can further provide a holistic, dynamic, and integrated decision-support to land conservation and rehabilitation projects in similar settings across South Africa and other developing countries as opposed to the more traditional one-dimensional approaches. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Sibiya, Silindile
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422470 , vital:71945
- Description: Soil erosion is a major global environmental problem and a pervasive forms of land degradation that threatens land productivity and food and water security. Some of the biggest sources of sediment in catchments are previously cultivated lands. Regardless of this factor, the abandonment of cultivated fields is not well-researched. Sustainable land management (SLM) interventions can play a significant role in mitigating and halting land degradation. This study investigated the dynamics of degradation exhibited by crop fields and the potential impacts of SLM interventions, using a leverage points framework and a case study in the Macubeni catchment of South Africa. The research answers three questions: (1) What is the relationship between the usage status of crop fields and degradation in Macubeni? (2) What are the drivers of crop field abandonment and how do they interact in the system? (3) Can proposed sustainable land management interventions tackle the dynamics of land abandonment, and associated degradation, at the root cause level? An empirical-analytical approach using a four step multi-method process was followed, in which crop fields were mapped using ArcGIS tools, literature was reviewed alongside stakeholder engagements, qualitative systems mapping modelling was undertaken, and a Multi-Criteria Analysis (MCA) with leverage points hierarchy was used to integrate all the steps together. The results revealed that the various drivers of crop field abandonment include natural environmental factors, socio-economic and social factors. 47.41% of the total crop fields in Macubeni were classified as highly degraded, and abandoned fields covered 37.47%. The statistical Chi-Square Test also confirmed that there is a significant relationship between the usage status and degradation level in crop fields. The SLM interventions assessed in the study have the potential to tackle the dynamics of land abandonment at a root cause level, however, there is a need to first shift the community’s mental models to address the existing sources of change resistance that are hindering successful implementation. Furthermore, the innovative multi-method approach applied in this study can further provide a holistic, dynamic, and integrated decision-support to land conservation and rehabilitation projects in similar settings across South Africa and other developing countries as opposed to the more traditional one-dimensional approaches. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Distribution and conservation status assessment of the freshwater fishes in the Krom River system in the Eastern Cape Province, South Africa
- Authors: Mthombeni, Annah
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422303 , vital:71929
- Description: The Cape Fold Ecoregion (CFE) contains the highest number of endemic freshwater fishes in Africa, south of the Zambezi River system. The CFE’s unique fish fauna has, however, suffered severe decline in historical distribution ranges and population sizes due to multiple impacts, with the two main threats being introduced invasive piscivores and habitat degradation. Growing evidence shows that biodiversity is being lost at unprecedented levels due to increased human pressure and demand for limited resources. Future projections also indicate high extinction risk, particularly for freshwater ecosystems, as a result of global climate change. Yet for many regions, including the CFE, there is still limited biodiversity knowledge to guide decision making processes. The Krom River system in the eastern Cape Fold Ecoregion (CFE) contains three native freshwater fish species. These are Pseudobarbus senticeps, which was recently revalidated, Sandelia capensis and a galaxiid lineage currently informally referred to as Galaxias zebratus sp. ‘Joubertina’. Two of these taxa are listed under threatened categories of the International Union for Conservation of Nature (IUCN). These are P. senticeps, which is listed as Critically Endangered and Galaxias zebratus sp. ‘Joubertina’, which is listed as Endangered. Although S. capensis is currently listed as Data Deficient, the most recent information indicates that the species comprises three allopatric lineages, with the Krom River population belonging to a lineage that is distributed across a number of river systems on the south coast. Two non-native fishes, Micropterus salmoides and Lepomis macrochirus, have also been introduced into this river system. Updated information on distribution patterns and habitat utilisation is required to guide authorities to develop effective management plans and conservation of the threatened fishes of the Krom River system. The aim of this study was to determine the current distribution of the fishes of the Krom River system and to provide recommendations for in situ conservation measures to protect remnant populations and promote recovery and range expansion of the native fishes. The primary objective of the study was to use historical and present data to map the distributions patterns. Data on the distribution of the fishes of the Krom River system were obtained from various sources, including the NRF-SAIAB database, studies published in peer reviewed literature and unpublished reports. Systematic sampling was conducted in the Krom River system and several of its tributaries to provide a snapshot of the current distribution of both native and non-native fishes in this system at the time of this study. The presence of instream physical barriers was recorded, and habitat and water quality were visually assessed. Only four species of freshwater fishes were recorded during the surveys. These were two native species, the Krom River redfin P. senticeps and the Cape kurper Sandelia capensis, and two introduced species, the black bass M. salmoides and bluegill sunfish L. macrochirus. Pseudobarbus senticeps was the most common and widely distributed species in the system, and was recorded at 20 localities (four mainstem and 16 tributary localities). Sandelia capensis was less common and was recorded at only eight localities (four mainstem and four tributary localities). These two native species co-occurred at all the eight localities where S. capensis was recorded, but P. senticeps was always more abundant than Sandelia. Micropterus salmoides was recorded at six localities (four mainstem and 2 tributary localities) while L. macrochirus was recorded at five localities (four mainstem and one off-stream dam site). Native fishes were not recorded at sites where non-native fishes were present except at two localities in the Wit Els River where juvenile M. salmoides were found amongst the native fish samples. However, unlike the other localities where all size classes (i.e., young of the year, juveniles, subadults and adults) were present, only adult redfins were found in the Wit Els River where juveniles of largemouth bass occurred. A comparison of the past and present distribution patterns of the native fishes indicates a considerable decline in distribution range, and remnant populations are now fragmented compared to past observations. The major threats and impacts on the Krom River system are the presence of non-native piscivores, construction of instream physical barriers, and agricultural activities. The information from this study could form a basis for establishing long-term conservation measures that should focus on preventing the spread of non-native fishes, and rehabilitating critical habitats for the future persistence of remnant populations of native fishes. The study also evaluated the implications of incomplete taxonomy on conservation status assessments and prioritisation, by evaluating case studies of species complexes of freshwater fishes whose taxonomy has been recently resolved, as well as two complexes with lineages that await formal recognition as distinct species. The aim was to demonstrate how incomplete knowledge of taxonomy affects the assessment of extinction risk and can potentially misdirect conservation prioritisation. Specifically, the study examined how change in taxonomy or recognition of undescribed genetic lineages in faunal listings affects range size and IUCN Red List risk category. The study taxa were Pseudobarbus afer sensu lato (sl), Enteromius anoplus sl, Amphilius natalensis sl, Sandelia capensis and S. bainsii, and the study assessed the species complexes as Least Concern or Near Threatened. The majority of these taxonomically revised species and genetic lineages were determined to be Critically Endangered (CR), Endangered (EN) and Vulnerable (VU), as the extent of occurrence was estimated to be < 100 km2; < 5000 km2 and < 20 000 km2 respectively. The species and lineages have been observed in fewer than ten locations, and the populations have been observed to be in decline. The results of this study indicate that the current taxonomic status of the native species in the Krom River system obscures the diversity of these fishes and affects conclusions for conservation assessments. Findings from this preliminary assessment highlight the need for advancing taxonomic knowledge through accurate delimitation of species boundaries, particularly within , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Mthombeni, Annah
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422303 , vital:71929
- Description: The Cape Fold Ecoregion (CFE) contains the highest number of endemic freshwater fishes in Africa, south of the Zambezi River system. The CFE’s unique fish fauna has, however, suffered severe decline in historical distribution ranges and population sizes due to multiple impacts, with the two main threats being introduced invasive piscivores and habitat degradation. Growing evidence shows that biodiversity is being lost at unprecedented levels due to increased human pressure and demand for limited resources. Future projections also indicate high extinction risk, particularly for freshwater ecosystems, as a result of global climate change. Yet for many regions, including the CFE, there is still limited biodiversity knowledge to guide decision making processes. The Krom River system in the eastern Cape Fold Ecoregion (CFE) contains three native freshwater fish species. These are Pseudobarbus senticeps, which was recently revalidated, Sandelia capensis and a galaxiid lineage currently informally referred to as Galaxias zebratus sp. ‘Joubertina’. Two of these taxa are listed under threatened categories of the International Union for Conservation of Nature (IUCN). These are P. senticeps, which is listed as Critically Endangered and Galaxias zebratus sp. ‘Joubertina’, which is listed as Endangered. Although S. capensis is currently listed as Data Deficient, the most recent information indicates that the species comprises three allopatric lineages, with the Krom River population belonging to a lineage that is distributed across a number of river systems on the south coast. Two non-native fishes, Micropterus salmoides and Lepomis macrochirus, have also been introduced into this river system. Updated information on distribution patterns and habitat utilisation is required to guide authorities to develop effective management plans and conservation of the threatened fishes of the Krom River system. The aim of this study was to determine the current distribution of the fishes of the Krom River system and to provide recommendations for in situ conservation measures to protect remnant populations and promote recovery and range expansion of the native fishes. The primary objective of the study was to use historical and present data to map the distributions patterns. Data on the distribution of the fishes of the Krom River system were obtained from various sources, including the NRF-SAIAB database, studies published in peer reviewed literature and unpublished reports. Systematic sampling was conducted in the Krom River system and several of its tributaries to provide a snapshot of the current distribution of both native and non-native fishes in this system at the time of this study. The presence of instream physical barriers was recorded, and habitat and water quality were visually assessed. Only four species of freshwater fishes were recorded during the surveys. These were two native species, the Krom River redfin P. senticeps and the Cape kurper Sandelia capensis, and two introduced species, the black bass M. salmoides and bluegill sunfish L. macrochirus. Pseudobarbus senticeps was the most common and widely distributed species in the system, and was recorded at 20 localities (four mainstem and 16 tributary localities). Sandelia capensis was less common and was recorded at only eight localities (four mainstem and four tributary localities). These two native species co-occurred at all the eight localities where S. capensis was recorded, but P. senticeps was always more abundant than Sandelia. Micropterus salmoides was recorded at six localities (four mainstem and 2 tributary localities) while L. macrochirus was recorded at five localities (four mainstem and one off-stream dam site). Native fishes were not recorded at sites where non-native fishes were present except at two localities in the Wit Els River where juvenile M. salmoides were found amongst the native fish samples. However, unlike the other localities where all size classes (i.e., young of the year, juveniles, subadults and adults) were present, only adult redfins were found in the Wit Els River where juveniles of largemouth bass occurred. A comparison of the past and present distribution patterns of the native fishes indicates a considerable decline in distribution range, and remnant populations are now fragmented compared to past observations. The major threats and impacts on the Krom River system are the presence of non-native piscivores, construction of instream physical barriers, and agricultural activities. The information from this study could form a basis for establishing long-term conservation measures that should focus on preventing the spread of non-native fishes, and rehabilitating critical habitats for the future persistence of remnant populations of native fishes. The study also evaluated the implications of incomplete taxonomy on conservation status assessments and prioritisation, by evaluating case studies of species complexes of freshwater fishes whose taxonomy has been recently resolved, as well as two complexes with lineages that await formal recognition as distinct species. The aim was to demonstrate how incomplete knowledge of taxonomy affects the assessment of extinction risk and can potentially misdirect conservation prioritisation. Specifically, the study examined how change in taxonomy or recognition of undescribed genetic lineages in faunal listings affects range size and IUCN Red List risk category. The study taxa were Pseudobarbus afer sensu lato (sl), Enteromius anoplus sl, Amphilius natalensis sl, Sandelia capensis and S. bainsii, and the study assessed the species complexes as Least Concern or Near Threatened. The majority of these taxonomically revised species and genetic lineages were determined to be Critically Endangered (CR), Endangered (EN) and Vulnerable (VU), as the extent of occurrence was estimated to be < 100 km2; < 5000 km2 and < 20 000 km2 respectively. The species and lineages have been observed in fewer than ten locations, and the populations have been observed to be in decline. The results of this study indicate that the current taxonomic status of the native species in the Krom River system obscures the diversity of these fishes and affects conclusions for conservation assessments. Findings from this preliminary assessment highlight the need for advancing taxonomic knowledge through accurate delimitation of species boundaries, particularly within , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Enhancing the use of large-scale assessment data in South Africa: Multidimensional Item Response Theory
- Authors: Lahoud, Tamlyn Ann
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422389 , vital:71938
- Description: This research aims to enhance the use of large-scale assessment data in South Africa by evaluating assessment validity by means of multidimensional item response theory and its associated statistical techniques, which have been severely underutilised. Data from the 2014 administration of the grade 6 Mathematics annual national assessment was used in this study and all analyses were conducted using the mirt package in R. A two parameter logistic item response theory model was developed which indicated a clear alignment between the model parameters and difficulty specifications of the test. The test was found to favour learners within the central band on the ability scale. An exploratory five dimensional item response theory model was then developed to investigate the alignment with the test specifications as evidence for construct validity. Significant discrepancies between the factor structure and the specifications of the test were identified. Notably, the results suggest that some items measured an ability that was not purely mathematical, such as reading ability, which would distort the test’s representation of Mathematics ability, disadvantage learners with lower English literacy, and reduce the construct validity of the test. Further validity evidence was obtained by differential item functioning analyses which revealed that fourteen items function differently for learners from different provinces. Although possible reasons for the presence of differential item functioning among provinces were not discussed, its presence provided sufficient evidence against the validity of the test. In conclusion, multidimensional item response theory provided an effective and rigorous approach to establishing the validity of a large-scale assessment. To avoid the pitfalls of the annual national assessments, it is recommended that this multidimensional item and differential item functioning techniques are utilised for the development and evaluation of future national assessment instruments in South Africa. , Thesis (MSc) -- Faculty of Science, Statistics, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Lahoud, Tamlyn Ann
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422389 , vital:71938
- Description: This research aims to enhance the use of large-scale assessment data in South Africa by evaluating assessment validity by means of multidimensional item response theory and its associated statistical techniques, which have been severely underutilised. Data from the 2014 administration of the grade 6 Mathematics annual national assessment was used in this study and all analyses were conducted using the mirt package in R. A two parameter logistic item response theory model was developed which indicated a clear alignment between the model parameters and difficulty specifications of the test. The test was found to favour learners within the central band on the ability scale. An exploratory five dimensional item response theory model was then developed to investigate the alignment with the test specifications as evidence for construct validity. Significant discrepancies between the factor structure and the specifications of the test were identified. Notably, the results suggest that some items measured an ability that was not purely mathematical, such as reading ability, which would distort the test’s representation of Mathematics ability, disadvantage learners with lower English literacy, and reduce the construct validity of the test. Further validity evidence was obtained by differential item functioning analyses which revealed that fourteen items function differently for learners from different provinces. Although possible reasons for the presence of differential item functioning among provinces were not discussed, its presence provided sufficient evidence against the validity of the test. In conclusion, multidimensional item response theory provided an effective and rigorous approach to establishing the validity of a large-scale assessment. To avoid the pitfalls of the annual national assessments, it is recommended that this multidimensional item and differential item functioning techniques are utilised for the development and evaluation of future national assessment instruments in South Africa. , Thesis (MSc) -- Faculty of Science, Statistics, 2023
- Full Text:
- Date Issued: 2023-03-29
Expression, partial characterisation and utilization of a GH11 xylanase (Xyn2A) from Trichoderma viride as an additive in monogastric animal feeds
- Mzimkulu-Ncoyi, Nosabatha Happyness
- Authors: Mzimkulu-Ncoyi, Nosabatha Happyness
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422409 , vital:71940
- Description: Endo-xylanases (shortly called xylanases) are a group of glycoside hydrolase enzymes that target β-D-1,4-linkages in the xylan backbone, leading to the production of xylooligosaccharides (XOS) of varying degree of polymerization (DP). Xylan is an indigestible non-starch polysaccharide present in monogastric animal feeds which in high amounts leads to increased digesta viscosity, slow movement of digesta in the intestines, malabsorption of nutrients among other challenges. The aim of this study was to investigate the effect of xylanase 2A (Xyn2A) from Trichoderma viride on broiler chicken feeds, particularly the hydrolysis of the xylan content, reduction of feed viscosity and the effect of produced XOS on eliciting the growth of gut associated probiotic bacteria. Xyn2AE was successfully induced with 0.8 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and produced in Escherichia coli BL21 (DE3) and Xyn2AC was expressed in tobacco mosaic plants. For the purification of Xyn2AE, an immobilized metal affinity chromatography (IMAC) column and diafiltration using a 3kDa cut-off Amicon filter membranes were used. Xyn2AE and Xyn2AC showed a xylanase active band at a relative weight of 21 kDa. Both enzymes showed high specificity towards soluble wheat arabinoxylan (WAX), with specific activities of 7.61 U/mg for Xyn2AE and 536.5 U/mg for Xyn2AC. Xyn2A kinetic parameters (Vmax and Km) were determined by Michaelis-Menten plots on soluble and insoluble WAX. The Vmax and Km values of Xyn2AC were 1003.01 U/mg and 9.25 mg/mL, 302.89 U/mg and 13.54 mg/mL, respectively. The Vmax and Km values of Xyn2AE for soluble and insoluble WAX were 20.45 U/mg and 12.95 mg/mL, and 8.31 U/mg and 13.15 mg/mL. Xyn2A enzymes displayed optimum activity at pH and temperature parameters of 5.0 and 50°C, respectively, and stability in temperatures ranging between 50 and 80°C and pH 4.0-9.0. Broiler chicken feeds were hydrolysed using Xyn2AE over a 24 h period and analysed using the dinitrosalicylic (DNS) assay, thin layer chromatography (TLC), viscometry and visualized using scanning electron microscope (SEM). The results showed a release of release of XOS xylotriose, xylopentose and xylohexose; enzyme’s ability to decrease the viscosity of the feeds and punched holes of feed surface, which was indicative of xylanase action. XOS produced during hydrolysis was used to study prebiotic effect on selected few bacteria and released short chain fatty acids (SCFAs) were measured. Additionally, SCFAs formation was detected in the presence of XOS as a carbon source for S. thermophilus and L. bulgaricus, whereas B. subtilis formed fewer organic acids in the presence of XOS. The results obtained from this study demonstrated that the supplementation of Xyn2A on broiler feeds has ii a positive effect in decreasing feed viscosity. Furthermore, the results of this investigation will assist the South African poultry farming sector to increase profitability in poultry farming and gain stability in the global trade as far as poultry feed is concerned. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Mzimkulu-Ncoyi, Nosabatha Happyness
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422409 , vital:71940
- Description: Endo-xylanases (shortly called xylanases) are a group of glycoside hydrolase enzymes that target β-D-1,4-linkages in the xylan backbone, leading to the production of xylooligosaccharides (XOS) of varying degree of polymerization (DP). Xylan is an indigestible non-starch polysaccharide present in monogastric animal feeds which in high amounts leads to increased digesta viscosity, slow movement of digesta in the intestines, malabsorption of nutrients among other challenges. The aim of this study was to investigate the effect of xylanase 2A (Xyn2A) from Trichoderma viride on broiler chicken feeds, particularly the hydrolysis of the xylan content, reduction of feed viscosity and the effect of produced XOS on eliciting the growth of gut associated probiotic bacteria. Xyn2AE was successfully induced with 0.8 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) and produced in Escherichia coli BL21 (DE3) and Xyn2AC was expressed in tobacco mosaic plants. For the purification of Xyn2AE, an immobilized metal affinity chromatography (IMAC) column and diafiltration using a 3kDa cut-off Amicon filter membranes were used. Xyn2AE and Xyn2AC showed a xylanase active band at a relative weight of 21 kDa. Both enzymes showed high specificity towards soluble wheat arabinoxylan (WAX), with specific activities of 7.61 U/mg for Xyn2AE and 536.5 U/mg for Xyn2AC. Xyn2A kinetic parameters (Vmax and Km) were determined by Michaelis-Menten plots on soluble and insoluble WAX. The Vmax and Km values of Xyn2AC were 1003.01 U/mg and 9.25 mg/mL, 302.89 U/mg and 13.54 mg/mL, respectively. The Vmax and Km values of Xyn2AE for soluble and insoluble WAX were 20.45 U/mg and 12.95 mg/mL, and 8.31 U/mg and 13.15 mg/mL. Xyn2A enzymes displayed optimum activity at pH and temperature parameters of 5.0 and 50°C, respectively, and stability in temperatures ranging between 50 and 80°C and pH 4.0-9.0. Broiler chicken feeds were hydrolysed using Xyn2AE over a 24 h period and analysed using the dinitrosalicylic (DNS) assay, thin layer chromatography (TLC), viscometry and visualized using scanning electron microscope (SEM). The results showed a release of release of XOS xylotriose, xylopentose and xylohexose; enzyme’s ability to decrease the viscosity of the feeds and punched holes of feed surface, which was indicative of xylanase action. XOS produced during hydrolysis was used to study prebiotic effect on selected few bacteria and released short chain fatty acids (SCFAs) were measured. Additionally, SCFAs formation was detected in the presence of XOS as a carbon source for S. thermophilus and L. bulgaricus, whereas B. subtilis formed fewer organic acids in the presence of XOS. The results obtained from this study demonstrated that the supplementation of Xyn2A on broiler feeds has ii a positive effect in decreasing feed viscosity. Furthermore, the results of this investigation will assist the South African poultry farming sector to increase profitability in poultry farming and gain stability in the global trade as far as poultry feed is concerned. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-03-29
Factors influencing the number of non-timber forest product species used at the individual, household and village levels in Vhembe, Limpopo Province, South Africa
- Authors: Mulaudzi, Takalani Rachel
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422481 , vital:71946
- Description: Non-Timber Forest Products (NTFPs) are wild biological resources obtained from natural landscapes, agricultural systems, and rural and urban regions for a variety of uses. NTFPs contribute significantly to livelihoods and living standards by offering a variety of socioeconomic benefits such as food, health, income generation, and safety nets. These multiple benefits are provided by a wide diversity of plant, animal and fungal species in different settings. However, the precise number of NTFP species used depends on the scale of assessment, namely use by individuals, by households and by villages. Consequently, it is difficult to compare numbers of species used between studies because of the differing scales. As a result, the objective of this study was to determine the number of NTFP species used at three different scales and how each is correlated with appropriate scale-dependent contextual variables. This objective was met by sampling individuals and whole households in five villages in the Vhembe District in Limpopo Province, South Africa. Twenty households were randomly selected in each village, providing a total sample of 100 households and 179 individuals in those households. In each household, every adult member was interviewed individually about the NTFPs that they used or knew. The major questions covered the NTFP species used, the respondents' socioeconomic level, the NTFP species used previously, and if the number of NTFP species are changing in the landscape. Data were reported per individual as well as per household and per village. The household level was derived by adding the responses of all the adults in each household but excluding duplicates. The same approach was used at the village scale, namely, the data across the 20 households were pooled, and duplicates were omitted. Patterns of NTFP use were examined by type of NTFP, and analysis using standard statistical tests, along with a Principal Components Analysis and a multivariate Detrended Correspondence Analysis of the NTFP species composition across villages. A total of 275 species of NTFPs were mentioned across the entire sample, including 81 species of firewood, 48 species of edible insects, 43 species of wild vegetables, 40 species of edible fruits, 38 species of wood utensils, 28 species of medicinal plants, 23 species of thatch grass, 19 species of poles, 19 species of wood for carvings, nine species of reeds, eight species of mushroom, and eight species of fodder. Of these species, some species had more than one use. Approximately 90% of the species used by the respondents were native species. Wild vegetables (e.g., Corchorus olitorius, Amaranthus capensis, Bidens pilosa), firewood (e.g., Colophospermum mopane, Parinari curatellifolia, Combretum hereroense), wild fruit (e.g., Landolphia kirkii, Vangueria infausta), and edible insects (e.g., Imbrasia belina, Macrotermes michaelseni, Encosternum delegorguei) were the most often used NTFPs by communities in the Vhembe area. Most of the households relied on NTFPs for subsistence. At the scale of an individual, the mean number of NTFP species used was 26.8±12.6 across the five villages. When considering a household, the mean number was 36.1±10.7 species. When aggregated across the 20 households per village, the mean number per village was 105.2±34.1 species. The species accumulation curve showed that 20 households per village were reasonably representative for four of the five villages. The results from the principal component analysis (PCA) and correlation analysis revealed that age, education, and employment status were significantly associated with the number of species used by individuals, whereas gender showed no clear correlation. At the household scale, household size was positively related with the number of species used, but income level and income source were not. At the village scale, attributes such as proximity to a town, settlement type, vegetation type, and distance to a nearby savanna all had some influence on the number of NTFP species listed. Most of the respondents felt that the number of species used had increased over the last 10–15 years, although some stated the opposite. Increases were attributed by traditional councils bans, the establishment of protected areas, and restoration. Activities eroding the number of NTFP species were identified as clear felling for farming, climate change, and over-harvesting. This study suggests that communities must be made more aware of the overharvesting of NTFP species in the savanna to avoid biodiversity loss. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Mulaudzi, Takalani Rachel
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422481 , vital:71946
- Description: Non-Timber Forest Products (NTFPs) are wild biological resources obtained from natural landscapes, agricultural systems, and rural and urban regions for a variety of uses. NTFPs contribute significantly to livelihoods and living standards by offering a variety of socioeconomic benefits such as food, health, income generation, and safety nets. These multiple benefits are provided by a wide diversity of plant, animal and fungal species in different settings. However, the precise number of NTFP species used depends on the scale of assessment, namely use by individuals, by households and by villages. Consequently, it is difficult to compare numbers of species used between studies because of the differing scales. As a result, the objective of this study was to determine the number of NTFP species used at three different scales and how each is correlated with appropriate scale-dependent contextual variables. This objective was met by sampling individuals and whole households in five villages in the Vhembe District in Limpopo Province, South Africa. Twenty households were randomly selected in each village, providing a total sample of 100 households and 179 individuals in those households. In each household, every adult member was interviewed individually about the NTFPs that they used or knew. The major questions covered the NTFP species used, the respondents' socioeconomic level, the NTFP species used previously, and if the number of NTFP species are changing in the landscape. Data were reported per individual as well as per household and per village. The household level was derived by adding the responses of all the adults in each household but excluding duplicates. The same approach was used at the village scale, namely, the data across the 20 households were pooled, and duplicates were omitted. Patterns of NTFP use were examined by type of NTFP, and analysis using standard statistical tests, along with a Principal Components Analysis and a multivariate Detrended Correspondence Analysis of the NTFP species composition across villages. A total of 275 species of NTFPs were mentioned across the entire sample, including 81 species of firewood, 48 species of edible insects, 43 species of wild vegetables, 40 species of edible fruits, 38 species of wood utensils, 28 species of medicinal plants, 23 species of thatch grass, 19 species of poles, 19 species of wood for carvings, nine species of reeds, eight species of mushroom, and eight species of fodder. Of these species, some species had more than one use. Approximately 90% of the species used by the respondents were native species. Wild vegetables (e.g., Corchorus olitorius, Amaranthus capensis, Bidens pilosa), firewood (e.g., Colophospermum mopane, Parinari curatellifolia, Combretum hereroense), wild fruit (e.g., Landolphia kirkii, Vangueria infausta), and edible insects (e.g., Imbrasia belina, Macrotermes michaelseni, Encosternum delegorguei) were the most often used NTFPs by communities in the Vhembe area. Most of the households relied on NTFPs for subsistence. At the scale of an individual, the mean number of NTFP species used was 26.8±12.6 across the five villages. When considering a household, the mean number was 36.1±10.7 species. When aggregated across the 20 households per village, the mean number per village was 105.2±34.1 species. The species accumulation curve showed that 20 households per village were reasonably representative for four of the five villages. The results from the principal component analysis (PCA) and correlation analysis revealed that age, education, and employment status were significantly associated with the number of species used by individuals, whereas gender showed no clear correlation. At the household scale, household size was positively related with the number of species used, but income level and income source were not. At the village scale, attributes such as proximity to a town, settlement type, vegetation type, and distance to a nearby savanna all had some influence on the number of NTFP species listed. Most of the respondents felt that the number of species used had increased over the last 10–15 years, although some stated the opposite. Increases were attributed by traditional councils bans, the establishment of protected areas, and restoration. Activities eroding the number of NTFP species were identified as clear felling for farming, climate change, and over-harvesting. This study suggests that communities must be made more aware of the overharvesting of NTFP species in the savanna to avoid biodiversity loss. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Implications of changing land use and introducing extralimital giraffe on vegetation in the subtropical thicket, South Africa
- Authors: Trafford, Bronwyn Kate
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422356 , vital:71935
- Description: Land use change has increased in southern Africa as many livestock farms convert to game reserves, to restore degraded and overgrazed lands. This leads to a change in herbivore communities with potential positive and negative consequences for vegetation and landscape productivity. This study aimed to look at the effect of land use change and subsequent herbivore community changes, on thicket vegetation structure, composition, and landscape productivity. To attain the study objectives, data were collected from four game reserves and their adjacent livestock farms within the Eastern Cape province in South Africa. These sites were selected because they are within the Subtropical Thicket biome (similar vegetation) and were once used for livestock farming. At each study site, vegetation composition and structure were assessed using a unique bush clump sampling technique along fence line contrasts. Six plots were sampled at each site, three plots on the game reserve lands and three plots on the adjacent livestock farmlands during the dry (July/August 2020) and wet (January/February 2021) seasons. The Normalized Difference Vegetation Index (NDVI), in-situ leaf area index (LAI) and the fraction of intercepted photosynthetically active radiation (FIPAR), were used to assess vegetation productivity between the two land use types. Additionally, diet of an extralimital herbivore, giraffe (Giraffa camelopardalis), was assessed during contrasting seasons (dry and wet) to infer the role this species might have on these landscapes. The diet of giraffes was assessed through field observations, recording plant species that giraffes were feeding on. Floristic results showed that there were no differences in vegetation structure, species composition and richness between the two land types. There was no difference in the overall diversity of plant species on the game reserves than in the adjacent livestock farms. The primary productivity was greater on the game reserves compared to the livestock farms. Field observations of giraffe feeding showed that they fed predominantly on Vachellia karroo followed by Searsia longispina. Their diets did not differ between the two seasons. My study provides an insight into the effects of changing land use and this information can be used to infer the extent to which these changes might have on restoration of degraded landscape and how land use changes may be effective in restoring the degraded thicket vegetation caused by overgrazing and overstocking of domestic livestock. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Trafford, Bronwyn Kate
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422356 , vital:71935
- Description: Land use change has increased in southern Africa as many livestock farms convert to game reserves, to restore degraded and overgrazed lands. This leads to a change in herbivore communities with potential positive and negative consequences for vegetation and landscape productivity. This study aimed to look at the effect of land use change and subsequent herbivore community changes, on thicket vegetation structure, composition, and landscape productivity. To attain the study objectives, data were collected from four game reserves and their adjacent livestock farms within the Eastern Cape province in South Africa. These sites were selected because they are within the Subtropical Thicket biome (similar vegetation) and were once used for livestock farming. At each study site, vegetation composition and structure were assessed using a unique bush clump sampling technique along fence line contrasts. Six plots were sampled at each site, three plots on the game reserve lands and three plots on the adjacent livestock farmlands during the dry (July/August 2020) and wet (January/February 2021) seasons. The Normalized Difference Vegetation Index (NDVI), in-situ leaf area index (LAI) and the fraction of intercepted photosynthetically active radiation (FIPAR), were used to assess vegetation productivity between the two land use types. Additionally, diet of an extralimital herbivore, giraffe (Giraffa camelopardalis), was assessed during contrasting seasons (dry and wet) to infer the role this species might have on these landscapes. The diet of giraffes was assessed through field observations, recording plant species that giraffes were feeding on. Floristic results showed that there were no differences in vegetation structure, species composition and richness between the two land types. There was no difference in the overall diversity of plant species on the game reserves than in the adjacent livestock farms. The primary productivity was greater on the game reserves compared to the livestock farms. Field observations of giraffe feeding showed that they fed predominantly on Vachellia karroo followed by Searsia longispina. Their diets did not differ between the two seasons. My study provides an insight into the effects of changing land use and this information can be used to infer the extent to which these changes might have on restoration of degraded landscape and how land use changes may be effective in restoring the degraded thicket vegetation caused by overgrazing and overstocking of domestic livestock. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
MeerKAT observations of three high-redshift galaxy clusters
- Authors: Manaka, Sinah Mokatako
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422367 , vital:71936
- Description: Galaxy clusters are the largest, gravitationally-bound structures in the Universe, formed through the hierarchical merger of smaller structures. The most accepted view is that the merging process injects energy into the intracluster medium (ICM) and re-accelerates pre-existing particles and compresses magnetic fields, generating, eventually, synchrotron emission. Such radio emission appears as radio halos, i.e. central Mpc-size diffuse structures, mostly visible in merging or unrelaxed clusters and with a spatial correspondence with the thermal gas component of the ICM. Observations have probed radio halo properties mostly for clusters withM500 > 6×1014 M⊙ at intermediate redshifts (0.3 < z < 0.4), providing support to their connection between mergers, which provide the necessary energy to re-accelerate particles via turbulence. Probing the redshift evolution of radio halos is an important test of the turbulent re-acceleration scenario, as fewer halos are expected at high redshift, given the same mass interval. In this thesis, we present MeerKAT observations at 1.28 GHz of three high-redshift (PSZ2G254.08- 58.45, PSZ2G255.60-46.18 and PSZ2G277.76-51.74, in the 0.42 ≲ z ≲ 0.46 range) clusters, with masses M500 ≳ 6.2 × 1014 M⊙, selected for their disturbed dynamical state – inferred from existing X-ray observations. Our observations reached rms noise values between 20 and 23.5 μJy beam−1, with ∼ 4′′ angular resolution. No evidence of diffuse emission is found at ii full resolution. Low-resolution (∼ 30′′) images achieved rms noise levels of 30-50 μJy beam−1, amongst the deepest observations of high-redshift targets. One radio halo was detected in the least massive cluster PSZ2G254.08-58.45 extending over ∼ 500 kpc, with a 1.20 } 0.08 mJy integrated flux density. We placed a ∼1 mJy upper limit at 95% confidence level on the radio halo flux density for the other two targets. The radio-halo detection is consistent with the recent P1.4 GHz − M500 correlation from Cuciti et al. (2021b), while the upper limit on PSZ2G255.60-46.18 is consistent with being on the correlation. On the other hand, the upper limit on PSZ2G277.76-51.74 places the radio halo well below the correlation. Recently a 1.5 GHz survey (Giovannini et al., 2020) detected a slightly higher fraction of radio halos in clusters in the same redshift range, with power and size typically higher than what we found in our observations. Both observations are, however, not inconsistent with each other. Our results, although with limited statistics, do not disfavour the current scenario of radiohalo formation based on the turbulent re-acceleration model. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Manaka, Sinah Mokatako
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422367 , vital:71936
- Description: Galaxy clusters are the largest, gravitationally-bound structures in the Universe, formed through the hierarchical merger of smaller structures. The most accepted view is that the merging process injects energy into the intracluster medium (ICM) and re-accelerates pre-existing particles and compresses magnetic fields, generating, eventually, synchrotron emission. Such radio emission appears as radio halos, i.e. central Mpc-size diffuse structures, mostly visible in merging or unrelaxed clusters and with a spatial correspondence with the thermal gas component of the ICM. Observations have probed radio halo properties mostly for clusters withM500 > 6×1014 M⊙ at intermediate redshifts (0.3 < z < 0.4), providing support to their connection between mergers, which provide the necessary energy to re-accelerate particles via turbulence. Probing the redshift evolution of radio halos is an important test of the turbulent re-acceleration scenario, as fewer halos are expected at high redshift, given the same mass interval. In this thesis, we present MeerKAT observations at 1.28 GHz of three high-redshift (PSZ2G254.08- 58.45, PSZ2G255.60-46.18 and PSZ2G277.76-51.74, in the 0.42 ≲ z ≲ 0.46 range) clusters, with masses M500 ≳ 6.2 × 1014 M⊙, selected for their disturbed dynamical state – inferred from existing X-ray observations. Our observations reached rms noise values between 20 and 23.5 μJy beam−1, with ∼ 4′′ angular resolution. No evidence of diffuse emission is found at ii full resolution. Low-resolution (∼ 30′′) images achieved rms noise levels of 30-50 μJy beam−1, amongst the deepest observations of high-redshift targets. One radio halo was detected in the least massive cluster PSZ2G254.08-58.45 extending over ∼ 500 kpc, with a 1.20 } 0.08 mJy integrated flux density. We placed a ∼1 mJy upper limit at 95% confidence level on the radio halo flux density for the other two targets. The radio-halo detection is consistent with the recent P1.4 GHz − M500 correlation from Cuciti et al. (2021b), while the upper limit on PSZ2G255.60-46.18 is consistent with being on the correlation. On the other hand, the upper limit on PSZ2G277.76-51.74 places the radio halo well below the correlation. Recently a 1.5 GHz survey (Giovannini et al., 2020) detected a slightly higher fraction of radio halos in clusters in the same redshift range, with power and size typically higher than what we found in our observations. Both observations are, however, not inconsistent with each other. Our results, although with limited statistics, do not disfavour the current scenario of radiohalo formation based on the turbulent re-acceleration model. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-03-29
Microbial and extracellular polymeric substance dynamics in arid–zone temporary pan ecosystems
- Authors: Bute, Tafara Frank
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422258 , vital:71925
- Description: Microbial communities of bacteria, viruses, algae, protozoans and fungi participate profoundly in aquatic systems, particularly in mediating processes such as primary production, decomposition, and biogeochemical cycles. In addition, microbiomes produce extracellular polymeric substances (EPS) which encompass a hydrated exopolymer mainly constituted of carbohydrates and proteins. The exopolymer aid proliferation and persistence of biofilms on their resident surfaces. There is however paucity of data on functional diversity of microbiomes in arid zone temporary wetlands with previous research having mainly focused on permanent systems in the northern hemisphere. In the face of ongoing climatic changes and anthropogenic threats to wetlands, it is imperative to assess the health status of aquatic systems in relation to microbial productivity dynamics. In this thesis, colorimetric methods and sequence–based metagenomics were conducted to quantify microbial EPS production and bacterial metagenome functions, respectively. This study was conducted in Khakhea–Bray region (North–West, South Africa) in June 2021 and January 2022 with a focus on evaluating microbial patterns of distribution between seasons (i.e., Dry and Wet) and varying depth i.e., deepest zones (Deep), intermediate depth (Mid) and shallowest regions (Edge). Additionally, potential relationships between EPS and either water content or organic matter content (OM content) were evaluated. In this study it was hypothesized that wet phases and deeper zones will have high EPS production and support more functions in comparison to shallowest regions and dry phases. Carbohydrates and proteins were quantified using the Dubois method and modified Lowry procedure, respectively. Carbohydrates generally occurred in higher proportions than proteins, suggesting that EPS found in these systems was largely diatom produced. The wet phases (wet season and inundation periods) supported more EPS production compared to the dry phases. The results of principal components analysis (PCA) and Spearman’s correlations suggested that EPS was highly correlated with sediment water content among other assessed variables. No significant associations were established between EPS and organic matter content. Spatial distribution of EPS demonstrated similar patterns between the deepest (Deep) and the intermediate depth zones (Mid) however the shallow regions (Edge) had significantly lower concentrations. Bacterial characterization was established by amplification of the 16S rRNA gene using illumina–sequencing protocol. Enzyme functions associated with biogeochemical pathways were predicted in PICRUSt2 bioinformatics pipeline. A total of 15 042 Unique Amplicon Sequence Variants (ASVs) were observed to be affiliated to 51 bacterial phyla and 1 127 genera. All top genera had commonality in heat tolerance. Firmicutes, dominated at phyla level with 59 % (mean ± sd, 19 ± 13 %) relative abundance followed by Actinobacteria and Proteobacteria both at 34 % (18 ± 7 %) and (18 ± 6 %), respectively. Microbial diversity matrices highlighted significant differences in beta diversity more than alpha diversity. Bacterial microbiomes were more distinct between seasons compared to within season, suggesting that functions were seasonally driven. These findings were supported by highest rates of denitrification, carbohydrate degradation and EPS production by core microbiomes in the wet season as compared to low rates of nitrogen mineralisation, carbon fixation and nitrification in the dry season. The present findings represent a first attempt in evaluating sequence–based metagenomics in semi–arid southern African temporary pan ecosystem. Both microbial EPS and bacterial functional potential were highly driven by water availability, with highest rates mainly associated with maximum inundation compared to dry states of pans. It can therefore be suggested that extended dry periods are threatening to microbially mediated processes in temporary wetlands, with implications to loss of biodiversity due to desiccation resulting in poor nutrient cycling. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Bute, Tafara Frank
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422258 , vital:71925
- Description: Microbial communities of bacteria, viruses, algae, protozoans and fungi participate profoundly in aquatic systems, particularly in mediating processes such as primary production, decomposition, and biogeochemical cycles. In addition, microbiomes produce extracellular polymeric substances (EPS) which encompass a hydrated exopolymer mainly constituted of carbohydrates and proteins. The exopolymer aid proliferation and persistence of biofilms on their resident surfaces. There is however paucity of data on functional diversity of microbiomes in arid zone temporary wetlands with previous research having mainly focused on permanent systems in the northern hemisphere. In the face of ongoing climatic changes and anthropogenic threats to wetlands, it is imperative to assess the health status of aquatic systems in relation to microbial productivity dynamics. In this thesis, colorimetric methods and sequence–based metagenomics were conducted to quantify microbial EPS production and bacterial metagenome functions, respectively. This study was conducted in Khakhea–Bray region (North–West, South Africa) in June 2021 and January 2022 with a focus on evaluating microbial patterns of distribution between seasons (i.e., Dry and Wet) and varying depth i.e., deepest zones (Deep), intermediate depth (Mid) and shallowest regions (Edge). Additionally, potential relationships between EPS and either water content or organic matter content (OM content) were evaluated. In this study it was hypothesized that wet phases and deeper zones will have high EPS production and support more functions in comparison to shallowest regions and dry phases. Carbohydrates and proteins were quantified using the Dubois method and modified Lowry procedure, respectively. Carbohydrates generally occurred in higher proportions than proteins, suggesting that EPS found in these systems was largely diatom produced. The wet phases (wet season and inundation periods) supported more EPS production compared to the dry phases. The results of principal components analysis (PCA) and Spearman’s correlations suggested that EPS was highly correlated with sediment water content among other assessed variables. No significant associations were established between EPS and organic matter content. Spatial distribution of EPS demonstrated similar patterns between the deepest (Deep) and the intermediate depth zones (Mid) however the shallow regions (Edge) had significantly lower concentrations. Bacterial characterization was established by amplification of the 16S rRNA gene using illumina–sequencing protocol. Enzyme functions associated with biogeochemical pathways were predicted in PICRUSt2 bioinformatics pipeline. A total of 15 042 Unique Amplicon Sequence Variants (ASVs) were observed to be affiliated to 51 bacterial phyla and 1 127 genera. All top genera had commonality in heat tolerance. Firmicutes, dominated at phyla level with 59 % (mean ± sd, 19 ± 13 %) relative abundance followed by Actinobacteria and Proteobacteria both at 34 % (18 ± 7 %) and (18 ± 6 %), respectively. Microbial diversity matrices highlighted significant differences in beta diversity more than alpha diversity. Bacterial microbiomes were more distinct between seasons compared to within season, suggesting that functions were seasonally driven. These findings were supported by highest rates of denitrification, carbohydrate degradation and EPS production by core microbiomes in the wet season as compared to low rates of nitrogen mineralisation, carbon fixation and nitrification in the dry season. The present findings represent a first attempt in evaluating sequence–based metagenomics in semi–arid southern African temporary pan ecosystem. Both microbial EPS and bacterial functional potential were highly driven by water availability, with highest rates mainly associated with maximum inundation compared to dry states of pans. It can therefore be suggested that extended dry periods are threatening to microbially mediated processes in temporary wetlands, with implications to loss of biodiversity due to desiccation resulting in poor nutrient cycling. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
Mineral paragenesis of olmiite/poldevaartite and rhodochrosite/shigaite occurrences in the Kalahari Manganese Field, and their relation to the formation of high-grade manganese ore of Nchwaning II mine, Black Rock, South Africa
- Authors: Opperman, Alicia
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422448 , vital:71943
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Geology, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Opperman, Alicia
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422448 , vital:71943
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Geology, 2023
- Full Text:
- Date Issued: 2023-03-29
Optimization of an intranasal levodopa nanocrystalline formulation for delivery to the brain
- Authors: Kakono, Chiedza
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/420709 , vital:71771
- Description: Thesis embargoed. Probable release date in 2025. , Thesis (MSc (Pharm)) -- Faculty of Pharmacy, Pharmacy, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Kakono, Chiedza
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/420709 , vital:71771
- Description: Thesis embargoed. Probable release date in 2025. , Thesis (MSc (Pharm)) -- Faculty of Pharmacy, Pharmacy, 2023
- Full Text:
- Date Issued: 2023-03-29
Pharmaco-chemical investigation of Erythrina caffra: extracts, isolated compounds and their biological activities
- Authors: Nogqala, Simnikiwe
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422459 , vital:71944
- Description: In this study, secondary metabolites isolated from Erythrina caffra, a medicinal plant indigenous to South Africa, were investigated. E. caffra is well-known for its healing properties and it is traditionally used for treating bacterial infections like tuberculosis (TB), abscesses, tooth aches and ear infections. Its extracts have also been used to treat cancer. Though many studies have been done on this plant, most of them tended to focus solely on the isolated compounds. In the present study however, extracts, fractions and isolated compounds from E. caffra were evaluated for their anticancer, anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity. The methanol crude extract (B1) from the stem bark of E. caffra was used to extract alkaloidic fractions (B2 and B3) using ethyl acetate and n-butanol respectively, a third fraction (B4) was also extracted using ethyl acetate this fraction was called a neutral fraction. The neutral fraction (B4) was fractionated and through a sequence of column chromatography three active secondary metabolites were isolated. The isolated compounds included Lupeol (1), stigmasterol (2) and 5,7-Dihydroxy-4'-methoxy-3',5'-diprenylflavanone (3). These isolated compounds were characterized and identified using spectroscopic techniques including IR, NMR and high-resolution Mass Spectrometry. Using the cell line HCC-70, isolated from a primary ductal carcinoma, in vitro anticancer assays were carried out on the crude extract from the bark, fractions, isolated compounds and an unseparated mixture of two compounds. These samples were also evaluated for their anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity activities. The crude extract inhibited the cell viability by over 30% and had no effect on the HeLa cells at concentrations of 20μM. Abyssinone V’ 4-methyl-ether (3) and the mixture of stigmasterol (2) and an unidentified compound exhibited potent anticancer activity against the HCC-70 cell line with IC50 of 18.05μM and 9.04μM respectively. Antibacterial assays were also carried out on the crude extracts, fractions and concoctions made from the fractions with the best activity combined with the ones that performed poorly. The concoctions were prepared as two separate series (S and N series). The crude extract inhibited more than 80% of the Staphylococcus aureus cells at a concentration of 20μM with only minimal damage to the HeLa cells. In the concoctions however, the N series managed to inhibit over 96% of the S. aureus while exhibiting no cytotoxicity towards HeLa cells. The extract and its fractions also showed good anti-oxidant activities. Molecular docking of these compounds was done on the Human estrogen receptor (PDB ID:3ERT) and Abyssinone V’ 4-methyl-ether (3) showed the best docking score of -6.6 Kcal/mol, for the simulation against Epidermal growth factor receptor (PDB ID: 1M17) Stigmasterol (2) showed the best docking score of -3.8 Kcal/mol. In silico docking on 3ERT and 1M17 were done to test the binding affinity of the isolated compounds to the proteins which are well known to be overexpressed in some types of cancer. Flavonoids isolated from Erythrina species have been reported to possess good antiplasmodial activity. However, due to the minute amounts isolated in the present study in-vitro assays could not be carried out. Nevertheless, in-silico assays were conducted on the most prominent protozoal parasite which causes malaria in the majority of African countries. In-silico simulations were done against Plasmodium falciparum protein (PDB ID: 7KJH), of the tested compounds Abyssinone V’ 4-methyl-ether (3) was found possess the best docking score of -4.4 Kcal/mol. The molecular docking of 7KJH was done to assess the inhibitory potential of the isolated compounds on protozoal parasites. Pharmacokinetic properties of the isolated compounds were also assessed in silico to assist in evaluating the drug likeness of these compounds. The compounds showed a percent human oral absorption of 100% except for Abyssinone V’ 4-methyl-ether (3), which showed 93.83%, this indicates a remarkable oral bioavailability. Stigamsterol (2) exhibited a Caco-2 cell permeability (QPPCaco) greater than 500 which indicates outstanding results for good intestinal absorption. The compounds also displayed a blood-brain partition co-efficient (QPlogBB) ranging from -1.433 to 0.128 suggesting they will have less potential to cross the blood-brain barrier, thus reducing any CNS related toxicity. Molecular networking of the crude extracts and the fractions was done through GNPS which allowed the identification of known compounds including one isolated in the present study, Abyssinone V’ 4-methyl-ether (3). Possible derivatives that have not been isolated from this plant before were also putatively identified. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Nogqala, Simnikiwe
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422459 , vital:71944
- Description: In this study, secondary metabolites isolated from Erythrina caffra, a medicinal plant indigenous to South Africa, were investigated. E. caffra is well-known for its healing properties and it is traditionally used for treating bacterial infections like tuberculosis (TB), abscesses, tooth aches and ear infections. Its extracts have also been used to treat cancer. Though many studies have been done on this plant, most of them tended to focus solely on the isolated compounds. In the present study however, extracts, fractions and isolated compounds from E. caffra were evaluated for their anticancer, anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity. The methanol crude extract (B1) from the stem bark of E. caffra was used to extract alkaloidic fractions (B2 and B3) using ethyl acetate and n-butanol respectively, a third fraction (B4) was also extracted using ethyl acetate this fraction was called a neutral fraction. The neutral fraction (B4) was fractionated and through a sequence of column chromatography three active secondary metabolites were isolated. The isolated compounds included Lupeol (1), stigmasterol (2) and 5,7-Dihydroxy-4'-methoxy-3',5'-diprenylflavanone (3). These isolated compounds were characterized and identified using spectroscopic techniques including IR, NMR and high-resolution Mass Spectrometry. Using the cell line HCC-70, isolated from a primary ductal carcinoma, in vitro anticancer assays were carried out on the crude extract from the bark, fractions, isolated compounds and an unseparated mixture of two compounds. These samples were also evaluated for their anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity activities. The crude extract inhibited the cell viability by over 30% and had no effect on the HeLa cells at concentrations of 20μM. Abyssinone V’ 4-methyl-ether (3) and the mixture of stigmasterol (2) and an unidentified compound exhibited potent anticancer activity against the HCC-70 cell line with IC50 of 18.05μM and 9.04μM respectively. Antibacterial assays were also carried out on the crude extracts, fractions and concoctions made from the fractions with the best activity combined with the ones that performed poorly. The concoctions were prepared as two separate series (S and N series). The crude extract inhibited more than 80% of the Staphylococcus aureus cells at a concentration of 20μM with only minimal damage to the HeLa cells. In the concoctions however, the N series managed to inhibit over 96% of the S. aureus while exhibiting no cytotoxicity towards HeLa cells. The extract and its fractions also showed good anti-oxidant activities. Molecular docking of these compounds was done on the Human estrogen receptor (PDB ID:3ERT) and Abyssinone V’ 4-methyl-ether (3) showed the best docking score of -6.6 Kcal/mol, for the simulation against Epidermal growth factor receptor (PDB ID: 1M17) Stigmasterol (2) showed the best docking score of -3.8 Kcal/mol. In silico docking on 3ERT and 1M17 were done to test the binding affinity of the isolated compounds to the proteins which are well known to be overexpressed in some types of cancer. Flavonoids isolated from Erythrina species have been reported to possess good antiplasmodial activity. However, due to the minute amounts isolated in the present study in-vitro assays could not be carried out. Nevertheless, in-silico assays were conducted on the most prominent protozoal parasite which causes malaria in the majority of African countries. In-silico simulations were done against Plasmodium falciparum protein (PDB ID: 7KJH), of the tested compounds Abyssinone V’ 4-methyl-ether (3) was found possess the best docking score of -4.4 Kcal/mol. The molecular docking of 7KJH was done to assess the inhibitory potential of the isolated compounds on protozoal parasites. Pharmacokinetic properties of the isolated compounds were also assessed in silico to assist in evaluating the drug likeness of these compounds. The compounds showed a percent human oral absorption of 100% except for Abyssinone V’ 4-methyl-ether (3), which showed 93.83%, this indicates a remarkable oral bioavailability. Stigamsterol (2) exhibited a Caco-2 cell permeability (QPPCaco) greater than 500 which indicates outstanding results for good intestinal absorption. The compounds also displayed a blood-brain partition co-efficient (QPlogBB) ranging from -1.433 to 0.128 suggesting they will have less potential to cross the blood-brain barrier, thus reducing any CNS related toxicity. Molecular networking of the crude extracts and the fractions was done through GNPS which allowed the identification of known compounds including one isolated in the present study, Abyssinone V’ 4-methyl-ether (3). Possible derivatives that have not been isolated from this plant before were also putatively identified. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-03-29
Photodynamic therapy using morpholine substituted porphyrins in the presence of cancer specific molecules linked to graphene quantum dots
- Magaela, Ngwanabjala Bridged
- Authors: Magaela, Ngwanabjala Bridged
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422486 , vital:71947
- Description: This thesis reports on the synthesis, characterization, photophysiochemical properties of morpholine substituted symmetrical and asymmetrical porphyrins. The synthesized porphyrins are conjugated to cancer selective biomolecules (folic acid and biotin) which are linked to nitrogen doped graphene quantum dots, as potential photosensitizers for photodynamic therapy (PDT). The symmetrical morpholine porphyrin complexes 2 (Sn(IV) 5,10,15,20 tetra-4-morpholinyl porphyrin) and 3 (Zn 5,10,15,20 tetra-4-morpholinyl porphyrin) had the same substituent but different central metals, and they were both conjugated to biotin decorated nitrogen doped graphene quantum dots (B-NGQDs), however complex 2 (Sn(IV) 5,10,15,20 tetra-4-morpholinyl porphyrin) was conjugated to B-NGQDs through an ester bond and complex 3 (Zinc 5,10,15,20 tetra-4-morpholinyl porphyrin) through 𝜋-𝜋 stacking. The effect of asymmetry was studied by comparing complex 3 (Zn 5,10,15,20 tetra-4-morpholinyl porphyrin) and complex 5 (Zn 5- bromophenyl-10-15-20-(tris-4- morpholinyl) porphyrin). Complex 5 (Zn 5- bromophenyl-10-15-20-(tris-4- morpholinyl) porphyrin) was an asymmetric porphyrin with morpholine and bromine as substituents. It was observed that asymmetry enhances singlet oxygen quantum yield and PDT activity. It was also observed that folic acid is a better targeting biomolecule when compared to biotin, and this was studied by comparing complex 3 conjugated to B-NGQDs and complex 3 conjugated to folic acid decorated nitrogen doped graphene quantum dots (FA-NGQDs). 3-FA-NGQDs had a better cellular uptake and PDT activity. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Magaela, Ngwanabjala Bridged
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422486 , vital:71947
- Description: This thesis reports on the synthesis, characterization, photophysiochemical properties of morpholine substituted symmetrical and asymmetrical porphyrins. The synthesized porphyrins are conjugated to cancer selective biomolecules (folic acid and biotin) which are linked to nitrogen doped graphene quantum dots, as potential photosensitizers for photodynamic therapy (PDT). The symmetrical morpholine porphyrin complexes 2 (Sn(IV) 5,10,15,20 tetra-4-morpholinyl porphyrin) and 3 (Zn 5,10,15,20 tetra-4-morpholinyl porphyrin) had the same substituent but different central metals, and they were both conjugated to biotin decorated nitrogen doped graphene quantum dots (B-NGQDs), however complex 2 (Sn(IV) 5,10,15,20 tetra-4-morpholinyl porphyrin) was conjugated to B-NGQDs through an ester bond and complex 3 (Zinc 5,10,15,20 tetra-4-morpholinyl porphyrin) through 𝜋-𝜋 stacking. The effect of asymmetry was studied by comparing complex 3 (Zn 5,10,15,20 tetra-4-morpholinyl porphyrin) and complex 5 (Zn 5- bromophenyl-10-15-20-(tris-4- morpholinyl) porphyrin). Complex 5 (Zn 5- bromophenyl-10-15-20-(tris-4- morpholinyl) porphyrin) was an asymmetric porphyrin with morpholine and bromine as substituents. It was observed that asymmetry enhances singlet oxygen quantum yield and PDT activity. It was also observed that folic acid is a better targeting biomolecule when compared to biotin, and this was studied by comparing complex 3 conjugated to B-NGQDs and complex 3 conjugated to folic acid decorated nitrogen doped graphene quantum dots (FA-NGQDs). 3-FA-NGQDs had a better cellular uptake and PDT activity. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-03-29
Prosecution of forced marriage by international courts and tribunals in light of the principle of legality
- Okeyo, Julian Rebecca Atieno
- Authors: Okeyo, Julian Rebecca Atieno
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/420720 , vital:71772
- Description: Thesis embargoed. Probable release date in 2024. , Thesis (LLM) -- Faculty of Law, Law, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Okeyo, Julian Rebecca Atieno
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/420720 , vital:71772
- Description: Thesis embargoed. Probable release date in 2024. , Thesis (LLM) -- Faculty of Law, Law, 2023
- Full Text:
- Date Issued: 2023-03-29
Quantifying feed intake and feeding intensity using two experimental conditions and the effect of different feeding strategies on the production parameters of farmed South African abalone, haliotis midae
- Authors: Wortley, Ross Michael
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:71932
- Description: As abalone are slow-growing animals there is a high investment of capital, running costs and labour in commercial abalone farming. Revenue needs to be maximised by achieving fast growth rates. A foremost driver of abalone growth is feed intake and feeding intensity of the abalone as well as the feeding regimen a farm utilises. While feed intake is well-documented in H. midae, there is a paucity of information regarding this aspect in abalone above 70 g and the relationship between feeding intensity, feed conversion rate (FCR) and daily growth rate needs to be documented. Similarly, there are a limited number of studies dealing with the effects of different pellet types of the same formulation, that differ only in shape and size, on feed intake and production in H. midae. This study made use of both a controlled laboratory experiment and commercial-scale farm experiment with the aim of testing whether feed intake information produced under laboratory conditions can provide good estimates of feed intake under farming conditions, which can further be implemented into feeding strategies on abalone farms. This study quantified daily feed intake (F) in 10 – 20 g, 100 – 110 g and 150 – 160 g abalone weight classes using two different pellet types and determined the relationship between the duration of feed availability and feed intake under laboratory conditions. The effect of three different pellet type strategies (fed daily: a leaf-only strategy = L d-1, a short-pellet only strategy = SP d-1, and a strategy that used both = B d-1) on abalone production parameters under farming conditions such as growth rate, feed conversion ratio (FCR), feeding intensity (FI) and weight variation of 30 – 40 g H. midae was also investigated. A commercial-scale farm study was designed to test the effect of four commercially relevant feeding regimens (three size-specific regimens and one commercially practiced feeding method) on the growth, FCR, feeding intensity and weight variation of these three abalone weight classes while taking into account an economic model to assess the profitability of each feeding regimen. Daily feed intake as a percentage of body mass (% BM d-1) was a function of abalone weight class between the 10 – 20 g and two larger weight classes 100 – 110 g and 150 – 160 g p < 0.0001), however feed intake was not different between the 100 – 110 g and 150 – 160 g weight classes. Pellet type did not affect feed intake in all abalone weight classes (p = 0.15). Feed intake (F) was positively linearly correlated with duration of feed availability (h) in the 10 – 20 g weight class (r2 = 0.76, p <0.00001) and logarithmically correlated in the 100 – 110 g (r2 = 0.25, p < 0.02) and 150 – 160 g (r2 = 0.52, p < 0.0001) weight classes (10 – 20 g abalone-1: 3 F (% BM) = 0.02(h) + 0.1976, 100 – 110 g abalone-1: F (% BM) = log10(h)*0.17 + 0.17, 150 – 160 g abalone-1: F (% BM) = log10(h)*0.36 + 0.07). There was no significant difference in monthly average abalone weight, daily growth rate (G), FCR and feeding intensity between each pellet type strategy (G: p = 0.60, FCR: p = 0.62, FI: p = 0.54 ). However, abalone grew well over the 112-day growth period with average abalone weight increasing significantly between each monthly sample time (pooled pellet type strategy: Huynh-Feldt correction; p < 0.00001). Furthermore, abalone fed the leaf-only pellet type strategy (L d-1) fell into higher weight classes after a 112-day growth period (Z test: 50 – 70 g: 72%) which was 16.1 % higher compared to the SP d-1 and B d-1 strategies. Daily growth rate (r2 = 0.34, p < 0.01) and FCR (r2 = 0.42, p < 0.004) was negatively correlated and FCR was positively correlated with daily feeding intensity, respectively (G = - 2.59 (FI) + 1.526, FCR = 8.8082 (FI) – 2.7108). Feeding regimen affected the production parameters of three abalone weight classes. The method practiced on the farm resulted in the best growth in the 10 – 20 g abalone weight class. The farm feeding method resulted in slower yet more efficient growth rates (lowest FCR values) in the abalone weight classes, 100 – 110 g and 150 – 160 g abalone- 1. However, size-specific feeding regimens resulted in the fastest growth rates but resulted in higher FCR values (less efficient). The relationship between feeding intensity, daily growth rate and FCR all showed that an increase in feeding intensity results in increased daily growth rates and FCR values. The economic model suggests that the higher FCR values associated with size-specific regimens, which have higher associated costs to producing abalone, were greatly outweighed by the growth attained by the abalone in the 100 – 110 g and 150 – 160 g weight classes. The size-specific regimens generated a higher potential monetary value of abalone after a 112-day period, which would consequently result in higher income for abalone farms. For abalone ranging from 100 – 110 and 150 – 160 grams, the economic model suggested that in a quarterly grading schedule (112 days) that abalone be fed the size specific daily rations, which is a function of body mass, at 0.35 % BM d-1 and 0.352 % BM d-1, respectively. The two experimental conditions used in this study produced feed intake and production parameter information that is beneficial to South African abalone farmers. The small-scale laboratory study produced information on feed intake that can be used as reference values as to what abalone in these weight classes can consume on a daily basis. The laboratory study can provide estimates of feed intake under farming conditions but should only be used as minimum 4 values when determining size-specific feeding regimens. To maximise abalone growth, farmers should utilise size-specific feeding regimens for abalone above 30 g. Daily growth rate and FCR can be predicted as a function of the abalone’s feeding intensity. Further studies are needed to determine the effects of abalone weight class on production parameters when testing different pellet types as well as an exploration into behavioural studies focusing on diet preferences. Additionally, future studies need to take into consideration abalone above the weight of 100 g with additional focus of research on behavioural, genetic and environmental aspects on abalone feed intake. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Wortley, Ross Michael
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:71932
- Description: As abalone are slow-growing animals there is a high investment of capital, running costs and labour in commercial abalone farming. Revenue needs to be maximised by achieving fast growth rates. A foremost driver of abalone growth is feed intake and feeding intensity of the abalone as well as the feeding regimen a farm utilises. While feed intake is well-documented in H. midae, there is a paucity of information regarding this aspect in abalone above 70 g and the relationship between feeding intensity, feed conversion rate (FCR) and daily growth rate needs to be documented. Similarly, there are a limited number of studies dealing with the effects of different pellet types of the same formulation, that differ only in shape and size, on feed intake and production in H. midae. This study made use of both a controlled laboratory experiment and commercial-scale farm experiment with the aim of testing whether feed intake information produced under laboratory conditions can provide good estimates of feed intake under farming conditions, which can further be implemented into feeding strategies on abalone farms. This study quantified daily feed intake (F) in 10 – 20 g, 100 – 110 g and 150 – 160 g abalone weight classes using two different pellet types and determined the relationship between the duration of feed availability and feed intake under laboratory conditions. The effect of three different pellet type strategies (fed daily: a leaf-only strategy = L d-1, a short-pellet only strategy = SP d-1, and a strategy that used both = B d-1) on abalone production parameters under farming conditions such as growth rate, feed conversion ratio (FCR), feeding intensity (FI) and weight variation of 30 – 40 g H. midae was also investigated. A commercial-scale farm study was designed to test the effect of four commercially relevant feeding regimens (three size-specific regimens and one commercially practiced feeding method) on the growth, FCR, feeding intensity and weight variation of these three abalone weight classes while taking into account an economic model to assess the profitability of each feeding regimen. Daily feed intake as a percentage of body mass (% BM d-1) was a function of abalone weight class between the 10 – 20 g and two larger weight classes 100 – 110 g and 150 – 160 g p < 0.0001), however feed intake was not different between the 100 – 110 g and 150 – 160 g weight classes. Pellet type did not affect feed intake in all abalone weight classes (p = 0.15). Feed intake (F) was positively linearly correlated with duration of feed availability (h) in the 10 – 20 g weight class (r2 = 0.76, p <0.00001) and logarithmically correlated in the 100 – 110 g (r2 = 0.25, p < 0.02) and 150 – 160 g (r2 = 0.52, p < 0.0001) weight classes (10 – 20 g abalone-1: 3 F (% BM) = 0.02(h) + 0.1976, 100 – 110 g abalone-1: F (% BM) = log10(h)*0.17 + 0.17, 150 – 160 g abalone-1: F (% BM) = log10(h)*0.36 + 0.07). There was no significant difference in monthly average abalone weight, daily growth rate (G), FCR and feeding intensity between each pellet type strategy (G: p = 0.60, FCR: p = 0.62, FI: p = 0.54 ). However, abalone grew well over the 112-day growth period with average abalone weight increasing significantly between each monthly sample time (pooled pellet type strategy: Huynh-Feldt correction; p < 0.00001). Furthermore, abalone fed the leaf-only pellet type strategy (L d-1) fell into higher weight classes after a 112-day growth period (Z test: 50 – 70 g: 72%) which was 16.1 % higher compared to the SP d-1 and B d-1 strategies. Daily growth rate (r2 = 0.34, p < 0.01) and FCR (r2 = 0.42, p < 0.004) was negatively correlated and FCR was positively correlated with daily feeding intensity, respectively (G = - 2.59 (FI) + 1.526, FCR = 8.8082 (FI) – 2.7108). Feeding regimen affected the production parameters of three abalone weight classes. The method practiced on the farm resulted in the best growth in the 10 – 20 g abalone weight class. The farm feeding method resulted in slower yet more efficient growth rates (lowest FCR values) in the abalone weight classes, 100 – 110 g and 150 – 160 g abalone- 1. However, size-specific feeding regimens resulted in the fastest growth rates but resulted in higher FCR values (less efficient). The relationship between feeding intensity, daily growth rate and FCR all showed that an increase in feeding intensity results in increased daily growth rates and FCR values. The economic model suggests that the higher FCR values associated with size-specific regimens, which have higher associated costs to producing abalone, were greatly outweighed by the growth attained by the abalone in the 100 – 110 g and 150 – 160 g weight classes. The size-specific regimens generated a higher potential monetary value of abalone after a 112-day period, which would consequently result in higher income for abalone farms. For abalone ranging from 100 – 110 and 150 – 160 grams, the economic model suggested that in a quarterly grading schedule (112 days) that abalone be fed the size specific daily rations, which is a function of body mass, at 0.35 % BM d-1 and 0.352 % BM d-1, respectively. The two experimental conditions used in this study produced feed intake and production parameter information that is beneficial to South African abalone farmers. The small-scale laboratory study produced information on feed intake that can be used as reference values as to what abalone in these weight classes can consume on a daily basis. The laboratory study can provide estimates of feed intake under farming conditions but should only be used as minimum 4 values when determining size-specific feeding regimens. To maximise abalone growth, farmers should utilise size-specific feeding regimens for abalone above 30 g. Daily growth rate and FCR can be predicted as a function of the abalone’s feeding intensity. Further studies are needed to determine the effects of abalone weight class on production parameters when testing different pellet types as well as an exploration into behavioural studies focusing on diet preferences. Additionally, future studies need to take into consideration abalone above the weight of 100 g with additional focus of research on behavioural, genetic and environmental aspects on abalone feed intake. , Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
- Full Text:
- Date Issued: 2023-03-29
Selection of DNA aptamers against Cancer-Targeting Murine Antibodies
- Authors: Nisbet, Laura Jade
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422426 , vital:71941
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Nisbet, Laura Jade
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422426 , vital:71941
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-03-29
Semantic segmentation of astronomical radio images: a computer vision approach
- Authors: Kupa, Ramadimetse Sydil
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422378 , vital:71937
- Description: The new generation of radio telescopes, such as the MeerKAT, ASKAP (Australian Square Kilometre Array Pathfinder) and the future Square Kilometre Array (SKA), are expected to produce vast amounts of data and images in the petabyte region. Therefore, the amount of incoming data at a specific point in time will overwhelm any current traditional data analysis method being deployed. Deep learning architectures have been applied in many fields, such as, in computer vision, machine vision, natural language processing, social network filtering, speech recognition, machine translation, bioinformatics, medical image analysis, and board game programs. They have produced results which are comparable to human expert performance. Hence, it is appealing to apply it to radio astronomy data. Image segmentation is one such area where deep learning techniques are prominent. The images from the new generation of telescopes have a high density of radio sources, making it difficult to classify the sources in the image. Identifying and segmenting sources from radio images is a pre-processing step that occurs before sources are put into different classes. There is thus a need for automatic segmentation of the sources from the images before they can be classified. This work uses the Unet architecture (originally developed for biomedical image segmentation) to segment radio sources from radio astronomical images with 99.8% accuracy. After segmenting the sources we use OpenCV tools to detect the sources on the mask images, then the detection is translated to the original image where borders are drawn around each detected source. This process automates and simplifies the pre-processing of images for classification tools and any other post-processing tool that requires a specific source as an input. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Kupa, Ramadimetse Sydil
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422378 , vital:71937
- Description: The new generation of radio telescopes, such as the MeerKAT, ASKAP (Australian Square Kilometre Array Pathfinder) and the future Square Kilometre Array (SKA), are expected to produce vast amounts of data and images in the petabyte region. Therefore, the amount of incoming data at a specific point in time will overwhelm any current traditional data analysis method being deployed. Deep learning architectures have been applied in many fields, such as, in computer vision, machine vision, natural language processing, social network filtering, speech recognition, machine translation, bioinformatics, medical image analysis, and board game programs. They have produced results which are comparable to human expert performance. Hence, it is appealing to apply it to radio astronomy data. Image segmentation is one such area where deep learning techniques are prominent. The images from the new generation of telescopes have a high density of radio sources, making it difficult to classify the sources in the image. Identifying and segmenting sources from radio images is a pre-processing step that occurs before sources are put into different classes. There is thus a need for automatic segmentation of the sources from the images before they can be classified. This work uses the Unet architecture (originally developed for biomedical image segmentation) to segment radio sources from radio astronomical images with 99.8% accuracy. After segmenting the sources we use OpenCV tools to detect the sources on the mask images, then the detection is translated to the original image where borders are drawn around each detected source. This process automates and simplifies the pre-processing of images for classification tools and any other post-processing tool that requires a specific source as an input. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-03-29
Source of introduced populations of Hemidactylus mabouia (Moreau de Jonnès, 1818) into the Eastern Cape Province and the potential spread to other South African regions
- Authors: Jeme, Alungile
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422334 , vital:71933
- Description: Human movements globally have resulted in dispersal and introduction of terrestrial and marine organisms into areas outside of their native range. Species that have been introduced to new habitats can either be alien (not causing any harm to the new environment) or invasive alien species (destroying or competing with the indigenous species for resources). Investigating the source of these introductions is important as it provides baseline information about their biology and evolution. It also allows for better prevention measures for future invasions and for effective conservation strategies. In this study, I investigate the source of the introduced populations of tropical house geckos Hemidactylus mabouia in the Eastern Cape Province using two mitochondrial gene regions, ribosomal 16S and NADH dehydrogenase subunit 4 (ND4). We also look at the potential spread of these species using species distribution models (SDMs). The phylogeny showed that the introduced populations into the Eastern Cape were genetically similar to both H. mabouia lineages found in Central & Southern Africa. The phylogeny produced two clades from which the majority of the introduced samples were grouped in a clade with samples from Angola, DRC, and Mozambique, and only a few nested within the second clade with the South African native population. Haplotype networks from both genes also showed two clades, matching those found in the phylogeny. The introduced samples were associated with samples from Angola and those from South Africa (native) as these localities had a number of shared haplotypes. SDMs showed less evidence that these individuals could naturally move west down the coast as the probability of occurrence was below 0.4 from the Eastern Cape to the Western Cape. Our conclusion was that the introductions may be due to multiple avenues like species moving down the east coast or human translocation including trade routes. From this we can conclude that geckos are largely translocated through human movement as they are known for their opportunistic behaviours as they are commensal with humans. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Jeme, Alungile
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422334 , vital:71933
- Description: Human movements globally have resulted in dispersal and introduction of terrestrial and marine organisms into areas outside of their native range. Species that have been introduced to new habitats can either be alien (not causing any harm to the new environment) or invasive alien species (destroying or competing with the indigenous species for resources). Investigating the source of these introductions is important as it provides baseline information about their biology and evolution. It also allows for better prevention measures for future invasions and for effective conservation strategies. In this study, I investigate the source of the introduced populations of tropical house geckos Hemidactylus mabouia in the Eastern Cape Province using two mitochondrial gene regions, ribosomal 16S and NADH dehydrogenase subunit 4 (ND4). We also look at the potential spread of these species using species distribution models (SDMs). The phylogeny showed that the introduced populations into the Eastern Cape were genetically similar to both H. mabouia lineages found in Central & Southern Africa. The phylogeny produced two clades from which the majority of the introduced samples were grouped in a clade with samples from Angola, DRC, and Mozambique, and only a few nested within the second clade with the South African native population. Haplotype networks from both genes also showed two clades, matching those found in the phylogeny. The introduced samples were associated with samples from Angola and those from South Africa (native) as these localities had a number of shared haplotypes. SDMs showed less evidence that these individuals could naturally move west down the coast as the probability of occurrence was below 0.4 from the Eastern Cape to the Western Cape. Our conclusion was that the introductions may be due to multiple avenues like species moving down the east coast or human translocation including trade routes. From this we can conclude that geckos are largely translocated through human movement as they are known for their opportunistic behaviours as they are commensal with humans. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2023
- Full Text:
- Date Issued: 2023-03-29
The development of a low-cost real- time bacterial plate culture monitoring system
- Authors: Songcata, Ntobeko
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422437 , vital:71942
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-03-29
- Authors: Songcata, Ntobeko
- Date: 2023-03-29
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422437 , vital:71942
- Description: Access restricted. Access embargoed until 2025. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-03-29