https://commons.ufh.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://commons.ufh.ac.za/vital/access/manager/Repository/vital:904 Wed 22 Jun 2022 12:38:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39543 Wed 13 Mar 2024 11:50:15 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:29717 Wed 13 Mar 2024 11:01:26 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10593 Wed 13 Mar 2024 09:41:38 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:5100 Wed 12 Oct 2022 11:53:52 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:804 Wed 12 Oct 2022 11:53:20 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3334 Wed 12 Oct 2022 09:37:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2917 Tue 15 Aug 2023 16:14:24 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2755 Tue 15 Aug 2023 15:50:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:5607 Tue 15 Aug 2023 10:51:57 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9960 Thu 13 May 2021 16:29:53 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11326 Thu 13 May 2021 16:27:28 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11665 Thu 13 May 2021 15:09:51 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39221 Thu 13 May 2021 14:10:26 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11575 Thu 13 May 2021 14:04:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3411 Thu 13 May 2021 13:55:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11269 Thu 13 May 2021 13:45:18 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2847 Thu 13 May 2021 13:03:24 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10185 Thu 13 May 2021 13:02:43 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11478 Thu 13 May 2021 13:01:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8489 Thu 13 May 2021 12:55:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:28261 Thu 13 May 2021 12:40:41 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3733 Thu 13 May 2021 12:37:24 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8916 Thu 13 May 2021 11:45:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9147 Thu 13 May 2021 11:44:29 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:20620 Thu 13 May 2021 11:01:19 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9894 Thu 13 May 2021 10:40:58 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11202 Thu 13 May 2021 10:30:13 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2166 Thu 13 May 2021 10:12:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3616 Thu 13 May 2021 09:56:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:18436 Thu 13 May 2021 08:54:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3884 Thu 13 May 2021 08:52:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10273 Thu 13 May 2021 08:49:56 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:906 Thu 13 May 2021 08:49:38 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:4739 Thu 13 May 2021 08:48:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9436 Thu 13 May 2021 08:46:37 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11204 Thu 13 May 2021 08:46:01 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9964 Thu 13 May 2021 08:45:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:802 Thu 13 May 2021 08:25:50 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9996 Thu 13 May 2021 08:23:37 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11747 Thu 13 May 2021 08:22:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:1152 Thu 13 May 2021 08:21:03 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:21073 Thu 13 May 2021 08:20:31 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8478 Thu 13 May 2021 08:19:50 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3886 20% of its initial activity after 9 consecutive reuse cycles. When exposed to fixed concentrations of carbofuran or demeton-S-methyl sulfone, immobilised acetylcholinesterase showed similar inhibition characteristics to that of the free enzyme. The decrease in enzyme activity observed after immobilisation to the nanofibres may have been due to several reasons which include some enzyme molecules being immobilised in structural conformations which reduced substrate access to the catalytic site, participation of the catalytic residues in immobilisation and enzyme denaturation due to the reaction conditions used for acetylcholinesterase immobilisation. Similar observations have been widely reported in literature and this is one of the major drawbacks of enzyme immobilisation. In conclusion, nylon-6:chitosan electrospun nanofibres were shown to be suitable supports for facile acetylcholinesterase immobilisation and the immobilised enzyme has potential for use in pesticide detection. Future recommendations for this study include a comparative study of the GA cross-linking method for AChE immobilisation which will lead to more intensely bound enzyme molecules to prevent non-specific binding. An investigation into the effect of inhibitors on stored immobilised AChE, as well as reactivation and reuse studies, may also be useful for determining the cost-effectiveness of reusing immobilised AChE for pesticide detection in environmental water samples. Several models have been designed for the determination of the kinetic parameters for immobilised enzymes. These take into account the mass transfer resistance as well as the overall charge of the immobilisation matrix. The use of these models to analyse experimental data will give a clear understanding of the effects of immobilisation on enzyme activity]]> Thu 13 May 2021 08:19:14 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9561 Thu 13 May 2021 08:19:03 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9017 Thu 13 May 2021 08:18:35 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11367 Thu 13 May 2021 08:18:32 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11694 Thu 13 May 2021 08:17:55 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10293 Thu 13 May 2021 08:17:50 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9697 Thu 13 May 2021 08:17:41 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9464 Thu 13 May 2021 08:17:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:4740 Thu 13 May 2021 08:16:38 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:894 Thu 13 May 2021 08:15:54 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11140 Thu 13 May 2021 08:14:35 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9280 Thu 13 May 2021 08:14:15 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8317 Thu 13 May 2021 08:12:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9129 Thu 13 May 2021 08:11:55 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10753 Thu 13 May 2021 08:10:18 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8900 Thu 13 May 2021 08:10:09 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:5398 Thu 13 May 2021 08:09:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8311 Thu 13 May 2021 08:08:52 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10086 Thu 13 May 2021 08:07:58 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9137 Thu 13 May 2021 08:07:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2390 Thu 13 May 2021 08:05:47 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9965 Thu 13 May 2021 08:04:57 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11445 Thu 13 May 2021 07:41:48 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:4727 Thu 13 May 2021 07:40:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8825 Thu 13 May 2021 07:39:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9026 Thu 13 May 2021 07:39:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8439 Thu 13 May 2021 07:38:29 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11703 Thu 13 May 2021 07:37:49 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11515 Thu 13 May 2021 07:37:41 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3609 Thu 13 May 2021 07:37:16 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11671 Thu 13 May 2021 07:36:36 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:4287 0.99. In the column experiment the highest loading capacity of Pt and Pd were 7.4 mg/g and 4.3 mg/g respectively on the nanofiber sorbent material based on ethylenediamine (EDA). The polystyrene and silica-based resins with triethylenetetramine (TETA) functionality (M-TETA and S-TETA) showed selectivity for platinum and palladium, respectively. Metal chlorido complexes loaded on the sorbent materials were recovered by using 3% m/v thiourea solution as teh eluting agent with quantitative desorption efficiency under the selected experimental conditions. The separation of platinum from palladium was partially achieved by selective stripping of PtCl₆²⁻ with 0.5 M of NaClO₄ in 1.0 M HCI with PdCl₄²⁻ was eluted with 0.5 M thiourea in 1.0 M HCI. The selectivity of the M-TETA and S-TETA sorbent materials was proved by column separation of platinum(IV) and palladium(II), respectively, from synthetic solutions containing iridium(IV) and rhodium(III). The loading capacity for platinum on M-TETA was 0.09 mg/g while it was 0.27 mg/g for palladium on S-TETA.]]> Thu 13 May 2021 07:36:09 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:836 Thu 13 May 2021 07:36:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9323 Thu 13 May 2021 07:35:43 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9713 Thu 13 May 2021 07:33:34 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9186 Thu 13 May 2021 07:33:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8885 Thu 13 May 2021 07:32:20 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10736 Thu 13 May 2021 07:32:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10360 Thu 13 May 2021 07:31:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:1827 Thu 13 May 2021 07:30:29 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:20462 Thu 13 May 2021 07:27:48 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:9441 Thu 13 May 2021 07:27:28 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:1388 Thu 13 May 2021 07:26:49 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:11585 15-35) and heavy invasions (>35% shrub cover) of E. floribundus. The average household size in the study area was 6.8± 0.7. All people in the three communal areas unanimously ranked sheep as the most important species for their livelihood, but the ranking of cattle and goat varied among the communal areas. All elder groups agreed that Europs floribundus decreases both the quantity and quality of herbaceous forage as well as livestock production. Moreover, invaded patches create more bare areas which are responsible for the loss of top fertile soils and the formation of rills and gullies. The result showed that the total density of E. floribundus significantly increased from the topland (2301 plants ha-1) moving to the bottomland (4888 plants ha-1). Canopy cover was significantly lowest in the topland (17.9%), but the remaining gradients had similar cover. Grass dry matter yield was higher in the bottomlands and sloppy gradients than the toplands. Soil organic carbon was significantly higher (P<0.05) in the flat than the sloppy terrain. Soils from higher elevations (toplands and upper sloppy) had significantly higher N than the lower elevations (bottomlands and Middle sloppy). The lowest dry matter was observed at low invasion and non-invaded sites. Soil chemical properties were generally different from all density levels. In conclusion, E. floribundus encroachment was found to be major cause of decline in the peoples’ livelihood because as this reduces the vegetation diversity and livestock production, both of which are the major of their livelihoods.]]> Thu 13 May 2021 07:26:36 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:1397 Thu 06 Jul 2023 12:12:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:1836 Thu 06 Jul 2023 12:06:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2891 Thu 01 Feb 2024 09:51:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2894 Mon 15 Aug 2022 13:28:48 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2388 Mon 12 Jun 2023 10:15:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2531 Mon 10 Oct 2022 14:29:46 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2530 Mon 10 Oct 2022 14:29:19 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:2612 Mon 10 Oct 2022 14:28:42 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:3557 Fri 30 Jun 2023 10:03:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:5097 Fri 15 Oct 2021 15:17:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:10295 Fri 15 Mar 2024 13:11:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:8354 Fri 15 Mar 2024 12:06:17 SAST ]]>