https://commons.ufh.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70172 Wed 29 Nov 2023 21:24:48 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70162 Wed 29 Nov 2023 19:41:31 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65371 Wed 26 Jul 2023 15:48:20 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65370 Wed 26 Jul 2023 15:13:29 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65368 Wed 26 Jul 2023 10:33:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65846 Wed 26 Jul 2023 10:19:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65367 Wed 26 Jul 2023 09:58:27 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65774 Wed 26 Jul 2023 09:16:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65767 Wed 26 Jul 2023 09:09:34 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:69920 Wed 24 Jan 2024 08:13:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65772 Wed 19 Jul 2023 14:31:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65765 Wed 19 Jul 2023 10:05:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70251 Wed 15 May 2024 19:18:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65722 Wed 12 Jul 2023 09:30:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65721 Wed 12 Jul 2023 09:19:30 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65720 Wed 12 Jul 2023 09:16:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65719 Wed 12 Jul 2023 09:10:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65718 Wed 12 Jul 2023 09:09:50 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65793 Wed 10 Jan 2024 11:15:28 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65374 Wed 10 Jan 2024 11:01:27 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61086 Wed 08 Feb 2023 13:07:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61101 Wed 08 Feb 2023 13:07:27 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61139 Wed 08 Feb 2023 13:07:26 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61140 Wed 08 Feb 2023 13:07:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61088 Wed 08 Feb 2023 13:07:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61100 Wed 08 Feb 2023 08:41:28 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:64750 Wed 07 Jun 2023 12:46:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:64749 Wed 07 Jun 2023 11:29:31 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70250 Wed 06 Dec 2023 17:40:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70257 Wed 06 Dec 2023 17:33:20 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70221 Wed 03 Jan 2024 13:48:30 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65790 Tue 25 Jul 2023 16:09:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:49968 100% inhibition in concentrations ≥ 30 μg/mL and TZDD1, 2 and 4 exhibited ≥ 50% inhibition activity in all the concentrations (10, 20, 30, 40 and 50 μg/mL) in the α-amylase inhibition assay. Similarly, in the α-glucosidase inhibition assay, all the four derivatives exhibited a concentration dependent activity with TZDD3 showing the most activity. All the four derivatives exhibited ≥ 30% inhibition in the aldose reductase inhibition assay except TZDD1 at 10 μg/mL. TZDD4 exhibited a concentration dependent inhibition activity in the protein tyrosine phosphatase-1B inhibition assay. Interestingly, TZDD3 showed a decreasing inhibition activity as its concentration increased from 10 μg/mL through to 50 μg/mL. In the dipeptidyl peptidase–4 inhibition assay, TZDD2 and TZDD4 exhibited ≥ 20% inhibition activity across all the concentrations and in the acetylcholinesterase assay, TZDD1, 3 and 4 exhibited ≥ 25% across all the concentrations. Interestingly, in the matrix metalloproteinase-1 inhibition assay, some of these derivatives exhibited partial activation activity and partial inhibition with TZDD1 showing activation in concentrations 10 and 20 μg/mL and inhibition in concentrations 30, 40 and 50 μg/mL. TZDD4 showed activation in all the concentrations. In the β-amyloid aggregation assay, all the four derivatives showed inhibition activity ≥ 10% except TZDD1 at 50 μg/mL. Conclusions Diabetes mellitus and Alzheimer’s disease are a type of pathology of global concern, and several researchers worldwide have strived to search for novel therapeutic treatments and prevention for diabetes as well as Alzheimer’s disease. Recent studies provide a direct link v between diabetes mellitus and Alzheimer’s disease, and the need to find novel drugs that can mitigate these two is of increasing interest. In our search for antidiabetic and anti-Alzheimer’s activity, TZD derivatives (TZDD1, TZDD2, TZDD3 and TZDD4) exhibited good antioxidant activity, anti-hyperglycaemic activity and a relatively promising anti-Alzheimer’s activity. This was observed from the in vitro evaluation performed which included α – amylase, α – glucosidase, aldose reductase, PTP1B, DPP4, amyloid β aggregation, and AChE inhibition assays. Furthermore, docking of the derivatives against PPARγ predicted a similar molecular interaction to that of thiazolidinediones using Rosiglitazone as the standard drug. Furthermore, in silico ADME profiling predicted the derivatives to have moderate to good solubility in the GI (good GI bioavailability), and also exhibited excellent drug likeness. However, they are predicted not permeate the BBB. Further in silico studies and in vivo should be conducted to establish toxicities, as well as drug delivery to the brain for effective therapeutic effect against Alzheimer’s disease.]]> Tue 21 Nov 2023 19:57:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65378 Tue 16 Jan 2024 14:38:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65376 Tue 16 Jan 2024 14:30:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65377 Tue 16 Jan 2024 14:30:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:64758 Tue 16 Jan 2024 14:23:47 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70121 Tue 05 Dec 2023 17:47:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70219 Tue 05 Dec 2023 14:03:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70156 Thu 21 Dec 2023 16:35:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:69918 Thu 21 Dec 2023 16:08:45 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65805 Thu 20 Jul 2023 13:00:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65845 Thu 20 Jul 2023 11:57:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65776 Thu 13 Jul 2023 10:47:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65375 Thu 11 Jan 2024 18:15:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65775 100 kDa through ultracentrifugation. Mass spectrometry also detected the most abundant peak for all fucoidans to be around 700 Da (m/z). Extracted fucoidans inhibited the activity of α-glucosidase more strongly than the commercial anti-diabetic agent acarbose but were inactive on α-amylase. Fucoidans were also shown to be mixed inhibitors of α-glucosidase. Compellingly, fucoidans synergistically inhibited α-glucosidase in combination with the anti-diabetic agent acarbose, highlighting prospects for combination therapy. Finally, fucoidans demonstrated some anti-proliferative characteristics on HCT116 cancer cells by inhibiting their ability to adhere to the tissue culture plate matrix. Furthermore, some fucoidan extracts inhibited the migration of HCT116 cancer cells from 3D spheroids. Some of our fucoidan extracts also inhibited HCT116 colony formation, demonstrating inhibition of long-term cell survival. The E. maxima water extract also inhibited glucose uptake by HCT116 cells, thereby influencing the glycolytic flux. In conclusion, biologically active fucoidans were successfully extracted from South African brown seaweeds. These fucoidans demonstrated anti-diabetic and anti-cancer properties, revealing their relevance as potential drugs for these diseases.]]> Thu 11 Jan 2024 18:08:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65763 200 km). These characteristics provide opportunities for high levels of connectivity, and as such, the stock structure is not well understood. We applied an integrated, multidisciplinary approach to provide an improved understanding of the complex stock structure dynamics for Patagonian toothfish on the Patagonian Shelf, specifically in relation to the shelf, slope, and deep-sea plains around the Falkland Islands. Research results were focused on aspects pertaining to (1) geographic variation in phenotypic characters (otolith shape); (2) a description of the spatial-temporal distribution patterns; (3) the active movements of deep-sea adults (tag-recapture study); and (4) the identification of early life-history dispersal through otolith microstructure and microchemical chronologies. Results from the study indicate high regional connectivity during the early life-history stages derived from at least two spawning contingents into spatially discrete nursery areas (cohort groups) on the Falklands Shelf. Fish followed distinct ontogenetic pathways into deeper waters adjacent to the areas wherein juvenile settlement into a demersal habitat occurred. There is little to no evidence of mixing among cohort groups during their ontogenetic migration into deep-sea adult habitats, reflecting a mixed population based on oceanographically defined egg and larval dispersal. The majority of the adult component of the population continue to display high site fidelity. However, between 9 and 25% of the population, consisting predominantly of larger reproductively capable adults undertake long-distance dispersal behaviour, identified as home-range relocations from the adult deep-sea habitats towards three of the known southern spawning grounds in the region. Results are suggestive of a requirement for improved collaborative efforts for regionally-based management approaches with careful consideration of local stock contingents. Future monitoring and research priorities should focus on the identification of reproductive potential, dispersal pathways and settlement patterns of stock contingents to inform the dynamics of mixed stock origins across the Patagonian region. While many aspects regarding the stock structure remain unresolved, results derived from the current studies can be used to inform the development of management measures to ensure the continued recovery and sustainable management of Patagonian toothfish within the region.]]> Thu 11 Jan 2024 18:07:47 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:64756 Thu 11 Jan 2024 18:00:54 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:64751 Thu 08 Jun 2023 08:48:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70510 Thu 07 Mar 2024 09:43:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:69921 Thu 07 Dec 2023 19:04:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70224 Thu 07 Dec 2023 19:04:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70175 Thu 04 Jan 2024 11:00:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65372 Thu 04 Jan 2024 11:00:35 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61087 Thu 02 Mar 2023 14:38:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65379 Sun 21 Jan 2024 14:37:43 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:69915 Sun 21 Jan 2024 14:17:22 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70255 Sun 21 Jan 2024 14:17:15 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70159 Sun 21 Jan 2024 14:17:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70160 Sun 21 Jan 2024 14:17:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:69919 Sun 21 Jan 2024 14:16:49 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70246 Sun 21 Jan 2024 14:10:03 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70248 Sun 03 Dec 2023 19:12:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70223 Sun 03 Dec 2023 18:51:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70225 Sun 03 Dec 2023 18:50:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:49972 Sun 02 Jul 2023 12:24:22 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70157 Sat 23 Dec 2023 17:40:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:71954 Sat 06 Apr 2024 12:24:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:71953 Sat 06 Apr 2024 12:11:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65366 Mon 24 Jul 2023 15:34:14 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65365 Mon 24 Jul 2023 15:06:34 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65782 Mon 24 Jul 2023 09:30:24 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70174 Mon 18 Dec 2023 18:08:30 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:67488 Mon 18 Dec 2023 18:01:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70155 Mon 18 Dec 2023 18:00:49 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70253 Mon 18 Dec 2023 17:54:13 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70220 Mon 18 Dec 2023 12:52:38 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65764 Mon 17 Jul 2023 11:29:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65360 Mon 10 Jul 2023 08:48:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70254 Mon 08 Jan 2024 20:33:18 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65373 Mon 08 Jan 2024 12:35:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:61170 Mon 08 Jan 2024 12:35:27 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70173 Mon 04 Dec 2023 18:22:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70302 Mon 04 Dec 2023 18:09:09 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70218 Mon 04 Dec 2023 17:26:19 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65359 Mon 03 Jul 2023 14:52:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65358 Mon 03 Jul 2023 12:32:02 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65357 Mon 03 Jul 2023 10:33:08 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65356 Mon 03 Jul 2023 09:30:37 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70305 Fri 16 Feb 2024 17:40:17 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70300 Fri 16 Feb 2024 17:40:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70301 Fri 16 Feb 2024 17:39:52 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70303 Fri 16 Feb 2024 17:39:51 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70307 Fri 16 Feb 2024 17:39:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70310 Fri 16 Feb 2024 17:32:48 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:70252 Fri 08 Dec 2023 11:14:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65364 Fri 07 Jul 2023 14:38:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:65363 Fri 07 Jul 2023 14:24:16 SAST ]]> 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