https://commons.ufh.ac.za/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33519 Wed 12 May 2021 23:41:49 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34262 0.05) conception rate following oestrous synchronisation and timed artificial insemination. However, conception rate was not independent (P < 0.05) of provinces, body condition score and body frame size. Cows in KwaZulu-Natal (66 percent) had significantly higher (P < 0.05) conception rates than those in Limpopo (44 percent) and Mpumalanga (60 percent). Cows of body condition score of ≥ 3.5 (72 percent) had a significantly higher (P < 0.05) conception rate than those of body condition score of ≤ 2.5 (66 percent) and 3 (48 percent). Large (82 percent) framed cows had a significantly higher (P < 0.05) conception rate than small (49 percent) and medium (55 percent) framed cows. Cows in Mpumalanga had more chances to conceive than those of Limpopo and KwaZulu-Natal with odds ratio of 0.605 and 0.863, respectively. Cows in KwaZulu-Natal were in much better body conditions than those from the other two provinces. Cows of body condition score of ≥ 3.5 had more chances to conceive than those of body condition score of ≤ 2.5 and 3 with odds ratio of 0.592 and 0.388, respectively, and the difference was significant (P < 0.05). The study recorded a 100 percent calf survival rate following synchronisation and timed artificial insemination. The breed type, parity, age frame size and lactation status did not significantly influence calving response following oestrous synchronisation and timed artificial insemination (P > 0.05). However, calving rate was not independent of provinces, districts and body condition score (P < 0.05). Calving rate in Mpumalanga (58 percent) and KwaZulu-Natal (54 percent) was significantly higher (P < 0.05) than that recorded in Limpopo Province (36 percent). Calving rate of Gert Sibande (61 percent) and Ehlanzeni (50 percent) districts in Mpumalanga and Zululand (50 percent) and Harry Gwala (61 percent) in KwaZulu-Natal was significantly higher (P < 0.05) than that of the Capricorn (32 percent), Mopani (23 percent and Waterberg (30 percent) in Limpopo Province with the exception of Vhembe (44 percent). Cows with body condition score of ≤ 2.5 (60 percent) had a significantly higher (P < 0.05) calving rate than those with a body condition score of 3 (43 percent). Cows in Mpumalanga had more chances to calve than those in Limpopo and KwaZulu-Natal with odds ratio of 0.076 and 0.537, respectively. Additionally, quantitative data was collected through in-depth interviews using a semi-structured questionnaire. Data collected was managed and coded into themes using the Nvivo Version 11 software programme. Themes and issues that emerged were analysed and interpreted using critical social thinking and systems thinking. The results of the study revealed many factors that could compromise the implementation and adoption of ARTs in the study areas. The general feeling amongst cattle farmers interviewed was that government should address these challenges. A shortage of bulls was the main cause of the low cattle reproduction rate. The Nguni breed type cattle was perceived as the ideal cattle breed for rural areas by respondents. Oestrous synchronisation and artificial insemination can be applied under communal and emerging farming systems with success. From the study results, breeding with small framed animals such as the Nguni type breed under communal and emerging farming systems makes a lot of sense because the breed is known for its low feed maintenance requirement. Furthermore, cattle stakeholders should co-operate and work together to address many of the constraints facing cattle productivity and the implementation and adoption of ARTs in rural areas.]]> Wed 12 May 2021 23:37:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33427 Wed 12 May 2021 23:18:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39802 Wed 12 May 2021 23:04:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39102 Wed 12 May 2021 22:58:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33128 Wed 12 May 2021 22:18:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39437 Wed 12 May 2021 20:13:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39218 Wed 12 May 2021 20:12:47 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:35478 Wed 12 May 2021 19:51:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:36450 Wed 12 May 2021 19:32:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33508 Wed 12 May 2021 19:25:58 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34818 Wed 12 May 2021 19:13:45 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34823 Wed 12 May 2021 19:07:45 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:35346 Wed 12 May 2021 18:50:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39217 Wed 12 May 2021 17:04:46 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:35635 Wed 12 May 2021 16:47:21 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:29430 Wed 12 May 2021 16:39:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34272 Wed 12 May 2021 16:28:14 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39110 Wed 12 May 2021 16:22:53 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33937 50 μg/mL (highest concentration tested) in the methanol extract. This study revealed that the sample has the potential in phytotherapy against oxidative stresses (inflammations) caused by free radicals. It could also be a useful herb in combating diseases caused by pathogenic organisms. The high anti-Mycobacterium tuberculosis activity observed in this study suggests it could be a plant with high promise in the fight against tuberculosis. The toxicity evaluation signifies it is safe for further plant-based pharmaceutical explorations. This study also validated some reported ethnopharmacological uses of this plant and call for its protection from indiscriminate destruction in rubber plantations and other crop gardens/plantations in the world.]]> Wed 12 May 2021 16:12:57 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34320 Wed 12 May 2021 15:48:53 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:41295 Wed 12 May 2021 15:09:32 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40090 .05). The model chi-square value of 43.47 (approximately 44% ) was significant. Thus, the indication is that the model has a good fit in predicting the possibility of a fuel retail outlet being located in the core or peripheral zone of the metropolis as determined by the explanatory variables (the independent variables accounted for about 44% of possible factors which determined whether a fuel retail outlets is located either within the core or peripheral zones of the metropolis. The remaining 56% are factors not considered in the study). The result gotten from the in depth interview sessions corroborated the findings from the tested hypothesis. The third objective was to examine the effects which the supply chain technique employed (either vertically integrated model or third-party arrangement) has on the achievement of efficient and effective distribution of the products. In order to achieve this objective, all the 389 registered members of Petroleum Tanker Drivers (PTD) association who are members of the PTD in the metropolis were sampled, 265 (around 70%) of the distributed questionnaires were retrieved and used for the analysis. In depth interview sessions were also conducted with three different managers of the three major conglomerates in the downstream sector of the Nigerian petroleum sector. The result of one-way between subjects (ANOVA) which was conducted to compare differences in the management and performances of haulage services outsourced to the 3PLS and those run under VIM showed that there was a significant effect of ownership type on management performance (F (2,262) = 60.26, p<.05). The null hypothesis was therefore rejected while the alternate hypothesis was accepted. The findings from the interview sessions posted similar results. The fourth and final objective was to analyze the effect that the socio-economic attributes of the participants (haulers and managers of retail outlets) involved in the distribution of xxvi petroleum products has on the safe, efficient and cost-effective distribution of product and this was done through the use of descriptive statistics, inferential statistics and incisive analysis of the interview sessions. A total number of 265 (70%) out of the 389 registered members of Petroleum Tanker Driver Association (PTDA) participated in the study. Multiple regression analysis (MRA) was used to test this hypothesis. The result from the statistical analysis show that the use of stimulant, work condition, marital status and educational attainment/literacy level have joint influence on compliance (R2 = 0.31, F (4,260) = 29.72, p<.05). On the basis of the foregoing, the hypothesis is thus accepted. The result from the interview sessions confirmed the existence of strong relationship between the explanatory variables and the hypothetical dependant variable. The study recommended a review of the planning processes guiding the establishment of fuel retail outlets. The study also advocated that retailers should endeavour to have stronger control over the supply chain of their products this could done by investing more in VIM rather than outsourcing such functions to 3PLs. This will help in better business performances. Government agencies in charge of monitoring drug abuse among haulers are advised to be more proactive by the duo of prevention and punishment strategies. This should include advocacy campaign against drug abuse. Different ranges of punishments should also be meted out for individual haulers found culpable.]]> Wed 12 May 2021 15:06:55 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40506 Wed 12 May 2021 15:06:30 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40195 Wed 12 May 2021 15:06:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40516 Wed 12 May 2021 15:05:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40110 Wed 12 May 2021 15:04:41 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40152 Wed 12 May 2021 15:01:44 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40204 Wed 12 May 2021 15:01:15 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:42543 Wed 12 May 2021 14:55:55 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40406 Wed 12 May 2021 14:50:52 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40192 Wed 12 May 2021 14:45:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40405 Wed 12 May 2021 14:44:25 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40513 Wed 12 May 2021 14:39:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40607 0.05) and values of >0.20 were recorded in 40 percent (108/277) of all the isolates. The resistant genes ampC, blaOXA, tetA, tetM, dfr1, sul1, sul2, ermB, nptII and strA were detected in two or more Vibrio isolates while the SXT intergrase was detected only in one isolate. Seven of the 18 extreme drug resistant isolates possessed 1–3 plasmids of 1.5–10 kb range. A total of 321 presumptive Staphylococcus aureus isolates were recovered by culture out of which 202 (62.9 percent ) were identified as S. aureus by PCR. Of these, 33 (16.3 percent ) strains were mecA positive while the rest, 169 (83.7 percent ) were mecA negative. The sensitivity and specificity of MRSA detection by use of cefoxitin disc (30 μg) test was 93.9 percent and 91.7 percent , that for oxacillin screen agar test (6 μg/mL) was 81.8 percent and 92.3 percent while that of BrillianceTM MRSATM 2 agar was 87.9 percent and 94.1 percent respectively. Staphylococcal enterotoxin SEE was detected in 12.1 percent (4/33) of total strains followed by SEA 3.0 percent (1/33), and SEC 3.0 percent (1/33). SEB and SED were absent. Six of the isolates had the PVL gene, giving a prevalence of 18.2 percent . The most frequently detected SCCmec type was SCCmec type III (17/33; 51.5 percent ), closely followed by SCCmec subtype IVc (10/33; 30.3 percent ) and the least were SCCmec types I and II with only 3.0 percent (1/33) each. The rest four (4/33; 12.1 percent ) did not belong to any of the above types and were therefore considered to belong to other subtypes of SCCmec type IV not tested in the study. For antibiogram patterns, 12 (36.4 percent ) strains were resistant to doxycyline, 10 (30.3 percent ) to trimethroprim-sulfamethoxazole, 9 (27.3 percent ) to tetracycline and only 1 (3.0 percent ) strain was resistant to ciprofloxacin. Nineteen (57.6 percent ) of the MRSA strains had vancomycin MIC of ≤ 2 μg/mL, 4 (12.1 percent ) with MIC range of 4-8 μg/mL, and 10 (30.3 percent ) with values ≥ 16 μg/mL. Overall, 27 (91.percent) isolates showed simultaneous resistance to 3 or more antibiotics. The resistant genes tetM, tetA, ermB, BlaZ femA were detected in two or more strains resistant to various antimicrobials. Resistance to rifampicin was not caused by mutations in the rpoB gene. Conclusions: To the best of our knowledge, this study is the first study reporting a broad, and diverse bacteria flora of skin, gill, and gut of the South African dusky kob as well as the first study on human pathogenic vibrios, and MRSA from marine finfish especially those from recirculatory aquaculture systems. Also, our study revealed that Vibrio species with pathogenic potential are present in marine dusky kob and its environs. Dusky kob is contaminated with CA- MRSA and HA- MRSA that may cause difficult to treat infections with regular antibiotics. Some of the MRSA strains contained toxins that can cause food-borne gastroeneteritis. The present study revealed that MRSA and Vibrio species are potential reservoirs for antibiotic resistant genes, which could be disseminated to other bacterial species probably through plasmids, a public health risk.]]> Wed 12 May 2021 14:37:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:42581 Wed 12 May 2021 14:36:26 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:41197 pan-frying in olive oil > grilled samples > boiling for best cooked product based on overall acceptability. Grilling and frying heart muscles resulted in the highest Ca (45.9 mg/kg) and Mg (98.9 mg/kg), whereas boiling led to the lowest Ca (41.1 mg/kg) and Mg (91.4 mg/kg) concentration. The highest concentrations of Fe and Zn these minerals were recorded in grilled (32.7 mg/kg) and fried samples (75.2 mg/kg). Zinc and Fe concentration observed in liver and heart muscles were higher than those found in breast and leg muscle. In conclusion, the results confirm the potential of MOLP for use as an alternative to antibiotics in maintaining broiler health, reducing mortalities and improving carcass characteristics and some meat quality traits.]]> Wed 12 May 2021 14:33:22 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40107 Wed 12 May 2021 14:31:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40512 Wed 12 May 2021 14:31:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40491 Wed 12 May 2021 14:30:31 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:41363 Wed 12 May 2021 14:25:59 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40403 Wed 12 May 2021 14:25:03 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40501 Wed 12 May 2021 14:22:16 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40606 Wed 12 May 2021 14:20:28 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40514 Wed 12 May 2021 14:11:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:42580 PdNi-rGO (8.4.%) > PdCo-rGO (6.56%) > Pt (5.7%) which were better than the platinum efficiency in the cobalt redox mediator. The higher efficiency in the cobalt redox mediator relative to the iodine electrolyte illustrates that they are viable alternatives to the, corrosive and volatile iodine. Obtained results show that, the high recombination rates between the photogenerated electrons and the oxidized dye molecule which have been reported to reduce power conversion efficiency in one electron redox mediators did not affect the performance of the cell. However, these higher recombination rates affected the ferrocene electrolyte leading to extremely poor efficiency metrics. The obtained results indicated that reduced graphene oxide supported PdNi-rGO, PdNiCo-rGO as well as the unsupported PdNi3 alloys could successfully be implemented as substitutes to the platinum counter electrode in dye sensitized solar cells. The application of the palladium alloys is vital for improving stability and power conversion efficiency, as well as reducing cost.]]> Wed 12 May 2021 14:01:24 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40332 MUFA>PUFA in descending order of concentrations of mean values (44.19:40.06:15.75, respectively), while the n-6: n-3 ratio was 12:1. In sensory evaluation, consumers indicated how they would prefer to pay premium for meat with guaranteed muscle and fat colour, low fat content, high marbling and tenderness. Consumers observed breed differences (P<0.05) in muscle fibre separation and muscle colour. The overall liking of each attribute was more on moderate bright cherry red lean colour, moderate yellow fat, very abundant marbling and slight separation of muscle fibres. Differences on discrete liking of each attribute among consumers had an effect on the overall liking of each steak with Hereford steaks being more preferred by consumers. It was concluded that meat from beef animals within the same feeding regime has a different fatty acid composition owing to different breed synthetic pathways and feed ingredients and that meat tenderness is not affected by the muscle nanostructure early post-mortem. However, during ageing, meat tenderness is directly linked to breed related myofibril structure changes in particular the myofibril diameter, spacing between myofibrils and their interaction; while the muscle texture features, fibre diameter, spacing between muscle fibres and sarcomere length explain the non-uniformity of beef tenderness. Also the organoleptic and health related quality of meat assumed more importance among consumers in sensory evaluation. Thus meat classification systems should include more credence aspects or indicators that support credence quality of meat]]> Wed 12 May 2021 13:57:19 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:42575 Wed 12 May 2021 13:57:09 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40190 Wed 12 May 2021 13:56:35 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40495 Wed 12 May 2021 13:54:22 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40106 Wed 12 May 2021 13:53:09 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:42542 0.05) from wethers on 4% FSF as well. The total protein concentration, albumin, total bilirubin, Na, K, glucose, cholesterol, and liver enzymes were normal for wethers. However, serum creatinine level was lower in wethers fed on 4% FSF than those on 0% FSF (P < 0.01). Haemonchus and Coccidian fecal egg counts were low in wethers fed diets with 2%, 4%, and 6 % FSF (P < 0.01) compared 11 with wethers fed with 0% FSF inclusion level during the same period. The body condition score of wethers fed on diets with 2%, 4%, and 6% FSF inclusion levels were higher than those on 0% (P < 0.05). Wool yield, staple length, the coefficient variation of the fibre diameter, and fibre of wethers fed on diets with FSF were higher than those without FSF (P < 0.05). The fibre diameter of the wethers fed on FSF supplemented diets was the same as those on 0 % FSF (P > 0.05). The wethers spent more time on FSF supplemented diets and consumed more feed compared to that without FSF supplemented (P < 0.05). The levels of FSF inclusion in the diet affected the enteric methane output (ppm-m), where 4 % FSF had the highest enteric methane output while wethers on 2 % FSF had the lowest methane output (P < 0.05). Resting wethers produced more methane (g/day) than those feeding or standing (P < 0.05). Increasing levels of FSF did not affect ruminal temperature and pH. Ammonia-N increased with increasing FSF except in wethers fed on a diet with 4% FSF (P < 0.01). The total molar concentrations of the wethers' volatile fatty acids decreased with increasing FSF levels (P > 0.05). The acetic propionic ratio of the wethers also decreased except at a 4% inclusion level. The in vitro true digestibility dry matter (IVTDDM), in vitro true digestibility neutral detergent fibre (IVTDNDF), and in vitro true digestibility acid detergent fibre (IVTDADF) of the wethers decreased up to 4% FSF inclusion but tended to increase at 6% inclusion. This study's result as one of the pioneer studies in Dohne-Marino wethers showed that FSF treatment has the potential to improve the nutritional status of the animal and the animal performance and wool quality. Health-wise, FSF decrease nematode population and boost animal immunity as seen in RBC and WBC counts. It also can play a major role in protecting the environment, as seen in its reduction in feacal and urinary nitrogen, which is heavily involved in environmental pollution. Result also confirmed that the best period to target for methane mitigation in ruminants is the resting period. In conclusion, the inclusion of FSF in the diet of Dohne-Merino wethers has the potential to improve the overall performance, with a 4% FSF inclusion level having optimal productivity. However, future research is required to investigate FSF's effect on meat quality, rumen microbial community, in-vivo digestibility, and milk production.]]> Wed 12 May 2021 13:48:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40094 Wed 12 May 2021 13:45:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40183 Tue 07 May 2024 11:09:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40400 0.05) in the physico-chemical characteristics of beef, game meat and ostrich droëwors; containing on average 25.8 ± 1.25 g/100 g moisture, 42.0 ± 0.10 g/100 g protein, 32.0 ± 1.68 g/100 g fat, 6.2 ± 0.13 g/100 g ash and 4.2 ± 0.10 g/100 g salt; with aw and pH of 0.79 ± 0.015 and 5.3 ± 0.05, respectively. During processing and storage, TBARS were higher (P < 0.05) in pork droëwors (maximum 3.83 mg MDA/kg DM) than in beef (maximum 0.99 mg MDA/kg DM). Moringa oleifera leaf powder contained high levels of TPC (7.5 ± 0.2 mg gallic acid eq/g) and substantial levels of α-tocopherol (76.7 ± 1.9 mg/100 g) and β-carotene (23.2 ± 2.8 mg/100 g). The FRAP, α-tocopherol and β-carotene content of pork droëwors increased (P < 0.05) proportionally with increasing levels of MLP inclusion. Lipid oxidation occurred more rapidly (P < 0.05) when MLP was not added and was similar (P > 0.05) for all MLP treatments. There was no significant effect of the inclusion of 0.75 g/100 g MLP on the drying curves and physico-chemical characteristics of the droëwors. The α-tocopherol content was higher (P < 0.05) and TBARS during storage were lower (P > 0.05) with MLP addition. The results of the current study give an overview of the composition of commercial droëwors and showed higher susceptibility to lipid oxidation in pork droëwors. Moringa oleifera leaf powder exhibited antioxidant activity in pork droëwors, inhibited lipid oxidation and increased the content of α-tocopherol in the product. It can be concluded that MLP could be used as a functional antioxidant additive in pork droëwors.]]> Tue 07 May 2024 11:01:32 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40402 Tue 07 May 2024 10:54:04 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40200 Tue 07 May 2024 09:51:06 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:40567 Tue 07 May 2024 09:16:11 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:30551 2 for Staphylococcus species, Acinetobacter species and Stenotrophomonas maltophilia suggest that they have arisen from high-risk sources where antibiotics are in constant arbitrary use resulting in high selective pressure. The presence of tetracycline resistance genes in Acinetobacter species justifies the observed phenotypic resistance to oxytetracycline and intermediate resistance to minocycline. High phenotypic resistance and the presence of some resistance genes in Staphylococcus species is a possible threat to public health and suggests animals to be important reservoirs of antibiotic resistance determinants in the environment. Indiscriminate use of antibiotics induces this kind of antibiotic resistance and should be discouraged. Personal hygiene is encouraged as it reduces the load of Acinetobacter species contacted from the environment that may be difficult to control. Commensal Stenotrophomonas maltophilia are as important as their clinical counterparts due to their roles in opportunistic infection, antibiotic resistance and their associated genes, especially sul gene. Personal hygiene is hereby advocated especially when in contact with soil, plants and plants’ rhizospheric soil.]]> Tue 07 May 2024 08:48:34 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:35249 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.]]> Thu 13 May 2021 08:24:35 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:29448 Thu 13 May 2021 07:09:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:30747 Thu 13 May 2021 06:17:36 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39100 0.05) between the three land management systems. Woody plant density of height classes were significantly different (P < 0.05) between three land management systems. The abundance of seedlings, saplings and young shrubs were significantly greater (P < 0.05) in the ranch than the game reserve and communal area. The mature trees had a significantly greater density (P < 0.05) in the game reserve than the ranch and communal area. Soil analysis results showed that Magnesium (Mg), Potassium (K), Manganese (Mn) and Copper (Cu) had similar (P > 0.05) concentrations in soils obtained from all land management systems in both seasons. Soil Calcium (Ca) levels showed differences between land management systems in winter only being greater (P < 0.001) in the game reserve than the ranch and the communal lands. Soil Phosphorus (P) and Iron (Fe) also showed variations between land management systems in winter being significantly greater (P < 0.001) in the ranch than the other land management systems. The concentration of soil K differed between the two seasons in soils collected from the ranch and the communal lands, being greater (P < 0.001) in winter than summer seasons. Soil Zinc (Zn) also showed differences between land management systems in summer being greater (P < 0.001) in the game reserve than in the ranch and communal land. Seasonal variations were observed in soil P level of samples collected from the ranch being greater (P < 0.001) in winter than summer season. The concentration of soil Zn was higher (P < 0.001) in the soil collected from the game reserve and the ranch in summer than winter season. Woody forage analysis results showed that browse samples harvested from the ranch had greater (P < 0.001) Nitrogen (N) level than the other land management systems in both seasons. The browse samples harvested from the game reserve had greater (P < 0.001) Ca level than the other land management systems in winter season only. The level of P showed variations (P < 0.05) between land management systems in samples harvested during winter only being greater (P < 0.001) in samples from the ranch than the other land management systems. Similarly, browse harvested during winter showed significant differences (P < 0.05) in K levels being lowest in samples collected from game reserves, where the Ca level was greatest. Browse samples collected from the communal land and the ranch had the lowest Mg levels in summer and winter seasons, respectively. In all management systems, browse harvested during summer had greater (P < 0.001) N and P levels than those harvested in winter, and the reverse holds true for K and Ca levels. Browse samples harvested during winter had greater (P < 0.001) Mn levels in samples from the communal area and game reserve than the ranch, but in summer, the game reserve had higher Mn level than the other two sites. Browse samples harvested during winter only showed differences (P < 0.05) in Cu and Zn levels being greatest (P < 0.001) in samples collected from ranch and game reserves. In all management systems, browse samples harvested during winter had greater (P < 0.001) Fe, Mn, Zn and Cu (except for the game reserve and communal land) levels than those harvested in summer. Herbaceous analysis results showed that samples harvested in summer from the game reserve and ranch had greater (P < 0.001) TOC levels than the communal area, but in winter, samples from the game reserve and ranch had similar TOC levels, except for communal areas that had no herbaceous vegetation. Herbaceous samples harvested in winter from the ranch had greater (P < 0.001) TN levels than the game reserve, and that made the C:N ratio higher (P < 0.001) in the game reserve. Woody foliar samples harvested in winter from the game reserve and communal lands had greater (P < 0.001) TOC than the ranch. Values on C:N ratio showed that in winter, the communal and ranch had greater (P < 0.001) C:N than the game reserve, but in summer, the ranch had higher (P < 0.001) C:N than the game reserve and communal area. The vegetation TOC (kg ha-1) showed the order of the game reserve > ranch > communal area and the trend was similar in both seasons. In summer, the game reserve had the greatest (P < 0.001) TN (kg ha-1) followed by the ranch and the lowest in the communal area, but in winter the game reserve and ranch had similar and higher (P < 0.001) TN (kg ha-1) than the communal areas. In summer, both the game reserve and ranch recorded greater (P < 0.001) herbaceous δ13C and lowest δ15N than the communal area, but in winter, the game reserve had higher (P < 0.001) δ13C than the ranch, though both the game reserve and ranch had similar (P > 0.001) δ15N values. In summer, both the communal and ranch had slightly higher (P < 0.001) woody foliar δ13C than the game reserve, but in winter the ranch had greater (P < 0.001) δ13C than game reserve and communal areas. Woody foliar samples harvested from the ranch showed variations (P < 0.05) in δ15N between seasons being higher (P < 0.001) in summer than winter. The TOC was greatest in summer, in soils collected from the game reserve followed by the ranch and least in the communal land, but in winter, the lowest TOC was recorded in soils collected from the ranch. The soil C:N ratio varied between land management systems in soils collected during summer only being greater (P < 0.001) in the game and ranch than the communal area. The soil TOC (kg ha-1) and TN (kg ha-1) did not show (P > 0.05) differences between land management systems. Soil δ13C level was remarkably (P < 0.05) affected by land management systems, but did not interact with seasons. In both seasons, the communal land had slightly higher (P < 0.001) δ13C level than the other two land management systems. In both seasons, δ15N levels showed great differences (P < 0.001) between land management systems, with highest soil δ15N levels observed in the communal area than the ranch and game reserve. Results on social survey showed that the majority of the interviewed households in Omaandi (63percent), Oshitutuma (53percent) and Epukunoyana (50percent) were female headed families and the remaining were male headed. The mean population of goats and cattle were significantly higher (P < 0.05) in Omaandi and Epukunoyana than in Oshitutuma village, respectively. In this study, household respondents in all villages indicated that all livestock species were primarily kept for wealth status and provision of meat for consumption. Respondents in all villages also indicated that lack of grazing lands and shortage of feed were the primary constraints of livestock production followed by water scarcity and recurring droughts. Elder respondents in all villages perceived overgrazing and climate change as the primary cause of rangeland degradation. Elder interviewees in Omaandi and Oshitutuma villages regarded low grass cover and dominance of poor quality grass (Epukunoyana only) as the primary vegetation indicators of rangeland degradation. The animal related indicators such as stunted animal body as a result of mineral deficiency, high livestock mortality, emaciated body and animals traveling long distance for grazing and water (Oshitutuma only) were regarded in all villages as the primary livestock related indicators of rangeland degradation. Elders from Epukunoyana village ranked low rainfall, high soil and atmospheric temperatures as the primary climate related indicators of rangeland degradation, although in Omaandi and Oshitutuma, high soil and atmospheric temperatures were considered as the primary climate related indicators of rangeland degradation. Based on the findings, this study concludes that land management systems had great influences on the distribution and production of herbaceous and woody vegetation in summer and winter seasons. This study also observed that land management systems interacted strongly with season to influence most vegetation and soil variables. Lastly, based on the study findings, the study concludes that all three communal rangelands are degraded and degradation led to a gradual declining trend in the livestock population over the past 10 to 20 years in the communal areas as a result of feed shortage.]]> Thu 13 May 2021 06:09:07 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39736 Thu 13 May 2021 06:02:03 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33113 Thu 13 May 2021 05:58:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:30764 0.05). Virulence factors are important for the pathogenesis of H. pylori. All the 48 clinical isolates were glmM (140 bp) positive and cagA was detected in 97.9 percent of the test isolates. The vacA gene was detected in all isolates but with different subtypes. The vacA allelic combination s1m1 was detected in 75 percent of the test isolates and s1m2 allelic combination was detected in 16.7 percent of the test isolates while the combination s2m2 was detected in 8.3 percent of the test isolates. None of the test isolates possessed the allelic combination s2m1. When H. pylori multiplication was monitored under acidic conditions in the presence of anti-urease active compounds, it was revealed that anti-urease active compounds in honey are capable of inhibiting the normal multiplication of H. pylori strains that are cagA positive, vacA positive and drug resistant. The GC-MS analysis showed that Fleures honey (urease I percent = 67.8 – 68.5 percent) and Gold Crest honey (urease I percent = 50.9 percent – 53.3 percent), all petroleum ether extracts had 27 and 26 volatile compounds. The hexane extract of Manuka honey (urease I percent = 50.0 – 53.2) had 43 compounds detected. The chloroform extract of Q Bee (urease I percent = 64.2 – 66.2 percent) had 13 volatile compounds detected. All the volatile compounds considered as representative samples of GC-MS analysis had a spectral matching ≥ 90 percent with the NIST11 library. However, the majority of compounds that were detected by LC-MS in representative honey extracts include vardenafil, urapidil, hydrocortisone, e.t.c which are drugs commonly used in the treatment of different ailments or infections that affect human beings. In addition, two xi drugs, sulfaquinoxaline and hydroxyquinoline which are used in veterinary medicine and antiseptic, disinfectant and pesticide applications in agricultural activities were detected in Little Bee honey. We therefore conclude that inhibition of urease has a bactericidal effect on drug resistant, cagA positive and vacA positive H. pylori strains growing under acidic environment.]]> Thu 13 May 2021 05:55:10 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:35146 Thu 13 May 2021 05:52:33 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34813 Thu 13 May 2021 05:24:20 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39464 Thu 13 May 2021 04:49:56 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39082 Thu 13 May 2021 04:41:12 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:31624 Thu 13 May 2021 04:33:23 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39801 Thu 13 May 2021 04:32:31 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39625 Thu 13 May 2021 04:30:01 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33423 Thu 13 May 2021 04:20:57 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34269 Thu 13 May 2021 02:40:39 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33400 1 mg/ml. Methanol extracts of both the wild and cultivated species exhibited almost the same level of toxicity (0.695 mg/ml and 0.628 mg/ml, respectively). Based on the Bastos criterion of toxicity indices of the lethality test, all the plant fractions demonstrated cytotoxicity effect, although the degree of their toxicity varied among the plants used. Standard spectrophotometry assays were used to quantify the phytochemical contents and to determine the antioxidant potentials of the cultivated and wild A. amatymbica. The results revealed different levels of phytochemicals with corresponding antioxidant activities. The wild extracts had higher phytochemical contents in most of the assays than cultivated extracts. Total phenol in the wild extracts ranged from 32.30±3.43 to 117.8±8.45 mg GAE/g with the acetone extracts having the highest content while the water extracts was the least. The range in the total phenol of the cultivated species was 66.46±5.29 to 98.44±6.08 mg GAE/g with the methanol extracts having the highest content while water extracts was the least. The flavonoids content ranged from 55.01±6.51 to 99.09±7.20 mg QE/mg and from 48.65±7.74 to 67.32±4.1 mg QE/mg for the wild and cultivated plants, respectively. The alkaloids contents ranged from 14.70±0.04 to 17.80±0.015 percent in the wild species while it ranged from 11.98±0.14 to 13.21±0.06 percent in the cultivated species. The wild species also showed higher antioxidant activities in most of the assays evaluated. The study revealed successful cultivation of the wild species of Alepidea amatymbica using fragments from the rhizome. The micromorphological study of both the wild and cultivated species showed minimal differences in the helical thickening of the walls in the xylem vessels and starch grain accumulation pattern. Although herbal medicine practitioners believe that cultivated species are not potent, however this study revealed some levels of phytochemicals contents and antioxidant activities which are comparable to the wild species. Cultivation is a major strategy in conserving nature and their resources. Hence, the successful domestication of A. amatymbica would help in reducing the pressure on the wild species while also meeting their medicinal demands purposes, utilisation and conserving for future generations.]]> Thu 13 May 2021 02:38:40 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:34307 0.05) effect on biomarkers of liver and renal damage. Haematological evaluation revealed that oral administration of aqueous extracts of K. africana, V. mespilifolia and the combination of both plants did not induce anaemia or leucocytosis in the animals. Furthermore, histopathological evaluation of the internal organs revealed no detectable inflammation at the the doses administered over a period of 28 days. These results demonstrated that the aqueous extracts of K. africana, V. mespilifolia and the combination of both plants was potentially safe for consumption orally even in chronic administration. Enzyme based in-vitro antiobesity evaluation of the aqueous and ethanolic extracts of K. africana, V. mespilifolia and their combination revealed that the ethanol extracts of both plants and their combination exhibited moderate inhibitory activities against α-amylase, α- glucosidase and pancreatic lipase. However, the standards used for the various inhibition assays exhibited much higher inhibitory actions when compared to any of the extracts. This suggests that the mechanism by which these two plants and their combination exert anti-obesity effects are probably not by inhibition of key enzymes of carbohydrate and fat metabolism.]]> Thu 13 May 2021 02:36:17 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:33431 Thu 13 May 2021 01:20:00 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:39799 Thu 13 May 2021 01:07:05 SAST ]]> https://commons.ufh.ac.za/vital/access/manager/Repository/vital:29092 Thu 13 May 2021 00:48:02 SAST ]]>